Lsi06G005530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G005530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionphytochromobilin:ferredoxin oxidoreductase, chloroplastic
Locationchr06: 6994361 .. 7000457 (-)
RNA-Seq ExpressionLsi06G005530
SyntenyLsi06G005530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCATCCTTACTTTAAATAACATTTTATCCGACTTGATTTCACCAATTTGGCGACGTGGGTCAACTCAGAATCCAAAATCCTTCTTCTTCAGCGGCTCAAACCCCAACAGTGGATCGATCGAATCGAGAGATAGAAAATGGGATTTTAGTAACGCAAATGCTTCAACTGAACCCTTCATTGGAAGCTTTGCTGCAATGGGTTCTTCTTCTCGTCTATCCTTCTCCCTTTCCTTCAACTTTCCACCATTTTCAAGCAACTGTAGCAGTAAAAACAAGTGTAAAAGAGCTTTCATTCCCATCTCTTCAATTTCATATCAGAAATTTGTTCAATTCGCTTTGGATGAAACCAAACTTCACACCCATCTCTTCCCTTCCCCTTTACAGGTAATGGGTCTTCTGATTTTGCTTCATTTGAGCTTCTCTAATATATTTTGCTTTGTAATGCTTCAAACAAAGTTTGGCTGTTGTTCTCTCCAGATTGGTATTTGTTTTCTTTGTTAAGTAGTGTCTGTGTAAGAATAGGGATGCTTTTTGTTTTTCTTGCAACTTTTAACTTCCTATCCCCTGTTCTACTGTGAGCTACTGAATGATGAGCTTTTTTTCAGGGCATTTTCAAGTTGTTTTACTGATTCTGTGTGAAGAGGTTTGATGGACTTGTTGTTTTAGAATAATTTTCATTGCTTTATCTTTAATGGACTGGAACTGACTCTCTCTAGCAAAGTATTCTGCGTTTTTAACTTGTCATAAGAATGCTCAGAAATTAATCTTTTGAGTCCTATTATCATTGGTCTGTCAAGTCATGCTAAATCAACTAATATTTGGGCAAAGTGAGATTAGTTCAACGGTAATTGGCATGACTTTCTTCTCTAGAGGTTGAAGCTTTGATTTCCATCTCTGCAATTGTTGTACTAAAAGAAAAAAACTAATATTTGTCCATTAGACTTACCACATCACTAATGATATTAATAACAGAGTCTACCTGGGATGGAATTGAAACTTTTGAAAGTAAATAAACACAACCATCAAACTTAATGGATTGAATTTGTAATTTAACTCTTGGTCCCTTTCTTGTCTATTGTCATTTTACGTAGAATTAATCTTAATTGTTTATTTTCTCTACATTTTATATTTCTTCAAACTTGCACATTTCCTTTTAGGGAGATTGTCGGATTGGTTTCTTGTTCTTATCTTGCTGGATTATAGCAGGTGTTTTTTTTCAACATAGATTCCATGTCTTTTTATGTCAGGATATTGAAGAAATCTAGCGATTCGTCAATCATATCTAAACTATTGCTTAAGATTATAGATTCATTTTTATCTAGAATGCACAGGTTGTCAACACACTAATTACTGAATACCCTAGTTGATATTTTAACTGATTGTATGATTTTATGTCAAGTCAAGTCAATATAATGCAATGTTTCAGGCTTGCATTTACATAAATGGTTGTGTAGTCTGTATCTTGGCTGTTATTGTCAGCATTCTTGATGTATTTCTGGTTGAAGTTTGTAAACCTTATGAATTAAATTTTAAAAAGTATATGAATTCAGACATGAATCATTGTTTCTGCCATCATATCTATATAATTATTCTTATCCTGCAGGAAAAATATAATTCCATGATTTCCTTGGACAAAAAAGTCAAGCTTGAAATGTTATCTTTCCAAGCTCCGAAGATCAGACTACTTCGCAGTTTGAATATTGAAAGTGAAACAATGCAGGTTTTATTTTATATTTCAACCCTCTTAGGAAGAACTTTTTATGCTTTAGTTCATATAATATGATTGGAAAATTAAATATAAGAGAATTTCAGCTGTATTAGGTATAGTGACTTGACAGAGGAGTATTGGAGGATTCTCTGACGATCACTTCTGTCTTTTTTGAGAAGGGAAACAAATTTTATTGACGAATGAAATAAAATGAGTAAACCTCAAAATGGATTACATGAAAACTTTCCAATTGGAAATCAGATACAAAAAACTATATTACAAAAGAAAGAAGTTTTATTAGTTCTCTGGCCCTTTTTACTTGTGGCCTTCTTTATTTTTCCTTGCTGAAATTCACTGCTTCCCCTTAAATTTAAAATTTAAAAATGTTAAAAGAACAACCATCCCTTGAGAAAGTTTTCCTTGGATATCAAAGTATAGAAATTTGCTAGTTATCCCAAGATAGTGTTGTAGTTTGTTGATGGTGAGAGGCTTAGTCTGAAGTCATAGACTTAAAAGTACTTGGTTATGGTTATGGGAATATTGATCCATGCAGCCATTCTTAGTAGAAAATCATTGTAAGAATTAATATCAATGATTTTGACGTTTCTGAATAATTTGGGACAGCTTCCAACAAATTTCTAGACCGCAGTTTGTGTAGCTAGCCCAGACGTAAATCTTATTTTCCTTTTTTGTAACTATTTTCTGCGGGTTAGAAATGCAAACCTTAAACTGCTGAAATTCATTCCTGAACCTGTGTGTGTTCAGTCACCATACTTAGTGTCTATGTTTATATTCTAAGAACTATTTCCATTCATTTATGGTGCAGGTTTTAGATTTTGCTGCATTCCCAGAACCAGAATTTGATGTTCCCATATTTTGCGCAAATATTTTCACTGCTGCAAGTTCAAGTATAGTAGTACTGTAAGCTTCTTTGATCTTTGGAGCCTGACAATAGTCTTGCATTATTTTTTCAATGTTTATACAAATTTTACATCGTCTACCTTGGAAGCATAGGAAATACTTAATTTGATGTTCTTGATATGAGAACATCAGTGATTCGGTCTAAACATCGGTCTAAAGTTCCATTGGTTGTGCGAATGCCTAGCACACTTTTAACGATAATATCCAACAAGTCAATTTAGTTCAACTCAAAGGATGGAGGTAGATACAGATTACCTCTAGGTCAAAACCTAGAAGAAAAAACCCAAATCTTGTTGGAAGGGGTCATATCTCTTATGGTTTTCCCAATTTCTCTCAGTTTGTTTGTTAGTTTCTGTTCTAGGAAAGGTTCAAAAATTTATTTTTAGGAGGACCATTGGGTGGGAGATAAAGCCTTTTTGTGTTTTATTTCCGCATTTACACTTTCTTTTGAACTAGATGTTGTGTTTAGTGGCCTCGGTGCTGCCTTCCCCGGACCATTCTTCTTTGATTTTATTAGGTTTGTGTCATCCTCAGTTTGATAGAGAGACGATAGATGTGGGGGGTCGTCTTTATATTTGTTGAGACCAGCACATGTTTCCATAAGAAGGTATATTAGGATTTGTACTCCTGATCCTTCTAGGTCTTTCTTTCCTACGTCAACTACTGCAGTTTTCACTTTTTTCCTAATTGTGGAAGGTTAAAATTCCGAAGAAGGTAAAGATTTTTGCATGGCAAGTTTTACATGGGAGGGCTAACACCATAGATCGTATTCAAAGACACTCTTCCTTTGTTTTGTACTAGGGTGCATTCTCTTTAGGAGGACAAGGAGGATCTTAATCATTTAGTGAATGTTTGGGGCAAGGGGTGGAGTTGTGAACTCAATGGAGTTATGAAGTTTGGGGAGTTATGAACTCCCAGGACTTACAGTATAAGGAGTTGATCAGGTATGAAATTGATATTTTTTAGTAGTGGGACCCACAAACTCCTTGGGTCAAACAAGGAGTGGAGTTCACAACTCCACACTTCCAACTTCCTACTCTCCAACTCCTTGACCGAAACACCCCCTTACTGTGGAGTTGCTAGTTTGCAAACTACCTTGCAGTCACTGGTTGAGTTCTTTTAGTGTTTGTGGGGCTCGCAACAGAGATAGGTGGTCTATATTGGAGAAGGTGCTTTTCGATCCTCCCTTTAAGGAGAGGGGCAAAGTTTTGTGGCATGCTAACTTCTTTGCTGTTTTGTGAGATATTTGGCTTGAGAAGAACAGTAGAACTTTTGGAGAGGTTGTGAAATCGAGTGAGGAAGTTTGGGAGGTGACGATGTTTAACGCTTATTTATGGGTATCAGTCACTAGGCCTTTTTGTAATTATAGTCTTGGTCTTGTTCTTTTAGATTGGAGTCCTTTTTTGAGGTTAGGGTTGGACTCTCATTTTTTTGGCATGTTTTTGTACGCCTTTGTATTCTTTCATTGTTCTCAATAAAAGCTTAGTTTCATACACCGCAAAAAAGGTTTGATGGCCAATCAATCTTAAAGCATGTCATCTAATCACAAGAATTGTTGATATTCATTTTTTATCCGCCACCTATATGTGCATAAATGTACAAATCTGCAATTTTTCCGACACTCATTAGGCTTAATAAGTTACATAGACAAGAGAATGTGGGCATCTTGTCTTCTGATTTAGTGAGCTTTCTCCAGTTTTTGTTGGTAGGCACTTGTTAGAAACTTAAGTAAGAGACGAATGGAAATGAAAATGACCAGAACTGATCCCATACGCAACTTTTTTAGCAATCTCAATCACATACCTGAAAAAAGAAAAAAAAAGGTTGATTTGGATTGAAATGAGTTTGCCTTATATGCATCCAGCGAGGGTTTTAACGCATGCATCAAATTGTGCTCCGTACACTTTGTATTCCGAGTGTCCAAATTTTATGTTAAAATATCTTCTCTAACTAGATTACCGGTTAATGTTCTTGTTCTTGCTGGAACCTACACTAAATATAATTTGCAACCATTCGTAACACTAACTCGGTTTTTTTTTTTTTTTTTTTAGGGATCTCAACCCTTTGCATGATGTTATCACCCAAAGAGATTATAGAGAGAAGTACTATAAAAGCTTATTGCATCTTGGGACTAAATATGCTGAGGTGTATGTTTTTTTTCTTAAGATATTAGTTTATATTACTACTACAGGCACTGTAGACTCGTTACTTTTATGACATTTGGTATACAACTTTTTTCCTTGATCTGTTCTAAATTTGATGTGATGATTACTGAAAACAGACTTCCTGCAGCTCTTACCCTGGGGCGGAAAGCTTACGAGCGAGTCGTTGAAATTTTTCTCTCCCATTGTGATATGGACCAAGTTTTCCTCAACCGAGTCCAAGTACGATATATTGTTTTCTGCATTCGTGGATTACTACAAGGTAATTGAAATTAACTATGCTGCTGCTATGATAAAGATTCCTATTTTTCCCCCCTTTTTGTGTGATTTCTAGCTCTGTCTTTTGAAGATTTTTAGTTATATGCATGTATTGGATCTTATTTTGATTATGTTACTGAAAGTTCCAATTTACTGCAAGGCATGGCTTGAGCTCGTAAACAATATTGAGGAGGAAACTATCCCGTCTCGACTTATGATTAATCGAGAAGCGCAACACAGATACCTGTTATGGAGGGCAGAAAAGGTAATAACATTTGTTTTCATATCATGGATCTCTTCTTCTTTCCTTTTATATTTTTGTTTTACGAAAATTTCCAACTTAGCTCTGGAGATCGGAGAAGCTCTGGTTGTATATGGATGGATATTACCAAAACTTAGCTAGATAATTACCCACAGTTGAACTTTGGTTGTAGTTTGTTCAAATTTGGAGCTGCAAGATCATTTATGAGTCTACTAAATTGCTTTTTTCTCTATACACAACTCCTGAAATAAATTTAATCTAGACTATAAAGTTTCATGAAACTCTCTCTTTTGTTAAAATTTCTGCTGACTTTATTCGATGCAACATTCACAAACAGGATCCGGGACACGGTCTTCTAAGAAAATTAATTGGGGAGACGCTTGCAAAGGTAACATACACGAAATCTGTCAATGTTCTTGCATCGTTTAAAAGTTTCATTCTCTCACTTCACATCTAACCATCAGGACCTGGTGAGAAATTTCCTGTTCAATGGCATCGACGAGCTCGGAAGCAAAAGGTTTCTGGATTACTTTCCCGAGTACTGTTGCGAAGATGGTACTATCAACACGAAACGCAGTATGGCTGGAAAGTCTTACGAACATCGACCTTGGGATAAAAGAGGTGAATTTATTGGAGATACTATCAAAAGCTGATGTTGGTTCCCTTCCTTACTGATATTTTTGGTTAAGTTAATGTAGGTGTACTATGTGAGAAGTTAGAATTATGTAACCTGTACATTAGACTGAGCTCAAATTTTGCTGATTTATTGTTGGCAAGCAAGTAG

mRNA sequence

TTTTCATCCTTACTTTAAATAACATTTTATCCGACTTGATTTCACCAATTTGGCGACGTGGGTCAACTCAGAATCCAAAATCCTTCTTCTTCAGCGGCTCAAACCCCAACAGTGGATCGATCGAATCGAGAGATAGAAAATGGGATTTTAGTAACGCAAATGCTTCAACTGAACCCTTCATTGGAAGCTTTGCTGCAATGGGTTCTTCTTCTCGTCTATCCTTCTCCCTTTCCTTCAACTTTCCACCATTTTCAAGCAACTGTAGCAGTAAAAACAAGTGTAAAAGAGCTTTCATTCCCATCTCTTCAATTTCATATCAGAAATTTGTTCAATTCGCTTTGGATGAAACCAAACTTCACACCCATCTCTTCCCTTCCCCTTTACAGGAAAAATATAATTCCATGATTTCCTTGGACAAAAAAGTCAAGCTTGAAATGTTATCTTTCCAAGCTCCGAAGATCAGACTACTTCGCAGTTTGAATATTGAAAGTGAAACAATGCAGGTTTTAGATTTTGCTGCATTCCCAGAACCAGAATTTGATGTTCCCATATTTTGCGCAAATATTTTCACTGCTGCAAGTTCAAGTATAGTAGTACTGGATCTCAACCCTTTGCATGATGTTATCACCCAAAGAGATTATAGAGAGAAGTACTATAAAAGCTTATTGCATCTTGGGACTAAATATGCTGAGGTACTTCCTGCAGCTCTTACCCTGGGGCGGAAAGCTTACGAGCGAGTCGTTGAAATTTTTCTCTCCCATTGTGATATGGACCAAGTTTTCCTCAACCGAGTCCAAGCATGGCTTGAGCTCGTAAACAATATTGAGGAGGAAACTATCCCGTCTCGACTTATGATTAATCGAGAAGCGCAACACAGATACCTGTTATGGAGGGCAGAAAAGGATCCGGGACACGGTCTTCTAAGAAAATTAATTGGGGAGACGCTTGCAAAGGACCTGGTGAGAAATTTCCTGTTCAATGGCATCGACGAGCTCGGAAGCAAAAGGTTTCTGGATTACTTTCCCGAGTACTGTTGCGAAGATGGTACTATCAACACGAAACGCAGTATGGCTGGAAAGTCTTACGAACATCGACCTTGGGATAAAAGAGGTGTACTATGTGAGAAGTTAGAATTATGTAACCTGTACATTAGACTGAGCTCAAATTTTGCTGATTTATTGTTGGCAAGCAAGTAG

Coding sequence (CDS)

ATGGGTTCTTCTTCTCGTCTATCCTTCTCCCTTTCCTTCAACTTTCCACCATTTTCAAGCAACTGTAGCAGTAAAAACAAGTGTAAAAGAGCTTTCATTCCCATCTCTTCAATTTCATATCAGAAATTTGTTCAATTCGCTTTGGATGAAACCAAACTTCACACCCATCTCTTCCCTTCCCCTTTACAGGAAAAATATAATTCCATGATTTCCTTGGACAAAAAAGTCAAGCTTGAAATGTTATCTTTCCAAGCTCCGAAGATCAGACTACTTCGCAGTTTGAATATTGAAAGTGAAACAATGCAGGTTTTAGATTTTGCTGCATTCCCAGAACCAGAATTTGATGTTCCCATATTTTGCGCAAATATTTTCACTGCTGCAAGTTCAAGTATAGTAGTACTGGATCTCAACCCTTTGCATGATGTTATCACCCAAAGAGATTATAGAGAGAAGTACTATAAAAGCTTATTGCATCTTGGGACTAAATATGCTGAGGTACTTCCTGCAGCTCTTACCCTGGGGCGGAAAGCTTACGAGCGAGTCGTTGAAATTTTTCTCTCCCATTGTGATATGGACCAAGTTTTCCTCAACCGAGTCCAAGCATGGCTTGAGCTCGTAAACAATATTGAGGAGGAAACTATCCCGTCTCGACTTATGATTAATCGAGAAGCGCAACACAGATACCTGTTATGGAGGGCAGAAAAGGATCCGGGACACGGTCTTCTAAGAAAATTAATTGGGGAGACGCTTGCAAAGGACCTGGTGAGAAATTTCCTGTTCAATGGCATCGACGAGCTCGGAAGCAAAAGGTTTCTGGATTACTTTCCCGAGTACTGTTGCGAAGATGGTACTATCAACACGAAACGCAGTATGGCTGGAAAGTCTTACGAACATCGACCTTGGGATAAAAGAGGTGTACTATGTGAGAAGTTAGAATTATGTAACCTGTACATTAGACTGAGCTCAAATTTTGCTGATTTATTGTTGGCAAGCAAGTAG

Protein sequence

MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPSPLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYERVVEIFLSHCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDKRGVLCEKLELCNLYIRLSSNFADLLLASK
Homology
BLAST of Lsi06G005530 vs. ExPASy Swiss-Prot
Match: Q9SR43 (Phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HY2 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 9.0e-83
Identity = 152/298 (51.01%), Postives = 205/298 (68.79%), Query Frame = 0

Query: 17  PFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPSPLQEKYNSMISLDKKV 76
           P   N + + + KR  + +S++SY++F + AL+ET+    L PS LQEKY+SM  LD K 
Sbjct: 28  PNKINFTLRRRKKRFLLRVSAVSYKEFAESALEETRKRIVLEPSHLQEKYSSMTGLDGKT 87

Query: 77  KLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDL 136
           +L+ML+F++ KIRLLRS+ IE+ETMQV DFA F EPE+D PIFCAN FT+ + +IVVLDL
Sbjct: 88  ELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTNVNIVVLDL 147

Query: 137 NPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYERVVEIFL---------S 196
           NPLH +  Q DY++KYY  ++ +  KYAE  P    L  ++ +    + +          
Sbjct: 148 NPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWTRFSSSKEK 207

Query: 197 HCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIG 256
           H  +   FL   QAWLE+   + EE  PS +  N EAQH+YL WRA+KDPGHGLL++L+G
Sbjct: 208 HKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGHGLLKRLVG 267

Query: 257 ETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDKRG 306
           E  AK+L+R+FLFNG+DELG+K F+DYFPEY  EDGT++ KRS+ GKSYE RPWD  G
Sbjct: 268 EAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETRPWDLTG 325

BLAST of Lsi06G005530 vs. ExPASy Swiss-Prot
Match: Q10UW6 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=pebB PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.5e-18
Identity = 63/238 (26.47%), Postives = 116/238 (48.74%), Query Frame = 0

Query: 40  YQKFVQFALDETKLHTHLFPSPLQEKYNSMISL------DKKVKLEMLSFQAPKIRLLRS 99
           YQ F+ +AL + K    L P P+   +   +++       ++V+    +  +PK+R +R+
Sbjct: 11  YQPFLDYALTKLKEELELQPYPIPSGFEHKVAMTGKGKKQQEVQTTSYAHCSPKLRQIRA 70

Query: 100 LNIE-SETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDLNPLHDVITQRDYREKY 159
            +++    +QVL+F  FP   +D+P F A++ +     ++ LD+ PL     Q  Y++KY
Sbjct: 71  AHVQGGSALQVLNFVIFPHLNYDLPFFGADLVSLPGGHLIALDMQPLFRDNLQ--YQKKY 130

Query: 160 YKSLLHLGTKYAEVLPAALTLGRKA---------YERVVEIFLSHCDMDQVFLNRVQAWL 219
            + +     K+ + LP       +A         + R  +  L    + + F + +QA+L
Sbjct: 131 TEPIWETFQKHQKYLPWGGDFPEEATPFFSPAFLWTRPQKTELVETRVFEAFKDYLQAYL 190

Query: 220 ELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIGETLAKDLVRNFLFN 262
             V   E+ T   +L   +EAQ RYL +RAEKDP   +L +  G    ++ +  FLF+
Sbjct: 191 IFVKQTEQITNSEKLAEIKEAQLRYLRYRAEKDPARKMLTRFYGHEWTEEYIHGFLFD 246

BLAST of Lsi06G005530 vs. ExPASy Swiss-Prot
Match: Q93TM8 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=pebB PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.6e-17
Identity = 60/238 (25.21%), Postives = 113/238 (47.48%), Query Frame = 0

Query: 40  YQKFVQFALDETKLHTHLFPSPLQEKYNSMISL------DKKVKLEMLSFQAPKIRLLRS 99
           YQ F+ +A+   +    L P P+   + S  ++       ++V     +FQ  K+R +R+
Sbjct: 11  YQPFLDYAIAYMRSRLDLEPYPIPTGFESNSAVVGKGKNQEEVVTTSYAFQTAKLRQIRA 70

Query: 100 LNIE-SETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDLNPLHDVITQRDYREKY 159
            +++   ++QVL+F  FP   +D+P F A++ T     ++ LD+ PL        Y+ KY
Sbjct: 71  AHVQGGNSLQVLNFVIFPHLNYDLPFFGADLVTLPGGHLIALDMQPLFR--DDSAYQAKY 130

Query: 160 YKSLLHLGTKYAEVLPAALTLGRKA---------YERVVEIFLSHCDMDQVFLNRVQAWL 219
            + +L +   + + L        +A         + R  E  +    +   F + ++A+L
Sbjct: 131 TEPILPIFHAHQQHLSWGGDFPEEAQPFFSPAFLWTRPQETAVVETQVFAAFKDYLKAYL 190

Query: 220 ELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIGETLAKDLVRNFLFN 262
           + V   E  T    L+  ++AQ RYL +RAEKDP  G+ ++  G    ++ +  FLF+
Sbjct: 191 DFVEQAEAVTDSQNLVAIKQAQLRYLRYRAEKDPARGMFKRFYGAEWTEEYIHGFLFD 246

BLAST of Lsi06G005530 vs. ExPASy Swiss-Prot
Match: Q7NL65 (Phycoerythrobilin:ferredoxin oxidoreductase OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=pebB PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.1e-15
Identity = 65/238 (27.31%), Postives = 109/238 (45.80%), Query Frame = 0

Query: 40  YQKFVQFALDETKLHTHLFPSPLQEKYNSMISL--DKKVKLEMLSFQAPKIRLLRSLNIE 99
           Y+ F++ A         L P P+   +    +L   + V     ++Q+PK+R +R+ +++
Sbjct: 4   YEPFLERAQQVLTRRLELAPYPIPVGFERKEALVRGEAVVTTSTAWQSPKLRQIRAAHVQ 63

Query: 100 -SETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDLNPL--HDVITQRDYRE---- 159
               +QVL+F   P  E+D+P F A++ T     ++ LD+ PL   D   Q  Y E    
Sbjct: 64  GGGALQVLNFVISPRLEYDLPFFGADLVTLPGGHLIALDMQPLFRDDPAYQAKYTEPIVP 123

Query: 160 --KYYKSLLHLGTKYAE-----VLPAALTLGRKAYERVVEIFLSHCDMDQVFLNRVQAWL 219
             + +++ L  G  + E       PA L      + R  E       +   F++ + A+L
Sbjct: 124 LFEAHRAHLEWGGDFPEEARPFFSPAFL------WTRPKETGTVETRVFAAFVDYLNAYL 183

Query: 220 ELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIGETLAKDLVRNFLFN 262
           + V   E  T P  L     AQ RYL +RAEKDP  G+ R+  G    ++ +  FLF+
Sbjct: 184 DFVERAEPVTHPEGLAAVERAQLRYLHYRAEKDPARGMFRRFYGPEWTEEYIHGFLFD 235

BLAST of Lsi06G005530 vs. ExPASy Swiss-Prot
Match: Q9K4U6 (15,16-dihydrobiliverdin:ferredoxin oxidoreductase OS=Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) OX=167539 GN=pebA PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 6.2e-07
Identity = 50/192 (26.04%), Postives = 85/192 (44.27%), Query Frame = 0

Query: 83  FQAPKIRLLRSLNIES-ETMQVLDFAAFPEPEFDVPIFCANI--FTAASSSIVVLDLNPL 142
           +  P  R  R   +++ + +QVL+  A+P    D PI   +I  F      + VLD  PL
Sbjct: 48  WDVPGFRRWRVTRMDAGDKLQVLNSVAYPAYTNDKPILGIDILWFGLKRKLVAVLDFQPL 107

Query: 143 HDVITQRDYREKYYKSLLHLGTKYAEV---LPAALTLGRKAYERVVEIFLS-----HCDM 202
              + +  Y  +YYK L  L  ++ +        +    K +   V ++        C +
Sbjct: 108 ---VQEERYFCRYYKDLQILKNRFVDFNSQKTMKIYDSNKYFSPWVLLYNGSFDDLQCSL 167

Query: 203 DQVFLNRVQAWLELVNNIEEE---TIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIGE 261
            ++    + A+ ++ NN   E    IPS++    +    Y ++ AE+DP HGL +   G+
Sbjct: 168 AKILDEFLHAYWQVDNNNSREYIKIIPSKV---EQLHINYDIYSAERDPAHGLFKSYFGQ 227

BLAST of Lsi06G005530 vs. ExPASy TrEMBL
Match: A0A6J1HBM1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461368 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.9e-136
Identity = 250/314 (79.62%), Postives = 270/314 (85.99%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           M S S LSF LSFN P  SS+CSSKNKC+ AFIP+S+ISY+KFVQFALDETKLHTHLFPS
Sbjct: 1   MASCSTLSFCLSFNSPLVSSSCSSKNKCRTAFIPVSAISYRKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMIS+DKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPE EFDVPIFC
Sbjct: 61  PLQEKYNSMISMDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAY 180
           ANIFTAASSSIVVLDLNPLHDVI QRDYREKYYKSL+HLGTKYAE+LP    LT     +
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSLMHLGTKYAELLPWGGKLTSESLKF 180

Query: 181 ERVVEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              + I+      +         F++  +AWLELV+N  EETIPSRLM+NREAQHRYL W
Sbjct: 181 FSPIVIWTKSSSTESTYDVLFSAFVDYYKAWLELVHNAAEETIPSRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGHGLL+KLIGETLAKDLVRNFLFNG+DELGSKRFLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGETLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDKRG
Sbjct: 301 AGKSYEHRPWDKRG 314

BLAST of Lsi06G005530 vs. ExPASy TrEMBL
Match: A0A6J1JIV1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111485492 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.6e-136
Identity = 249/314 (79.30%), Postives = 267/314 (85.03%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           M S S LSF LSFN  PFSS+CSSKNKC+RAF P+S+ISYQKFVQFALDETKLHTHLFPS
Sbjct: 1   MASCSTLSFCLSFNSTPFSSSCSSKNKCRRAFFPVSAISYQKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMIS+DK VKL+MLSFQAPKIRLLRSLNIESETMQVLDFAAFPE EFDVPIFC
Sbjct: 61  PLQEKYNSMISMDKNVKLQMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAY 180
           ANIFTAASSSIVVLDLNPLHDVI QRDYREKYYKSLLHLG KYAE+LP    LT     +
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSLLHLGIKYAELLPWGGKLTSESLKF 180

Query: 181 ERVVEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              + I+      +         F++  +AWLELVNN  EETIPSRLM+NREAQHRYL W
Sbjct: 181 FSPIVIWTKSSSTESTYDILFSAFVDYYKAWLELVNNAAEETIPSRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGHGLL+KLIGE LAKDLVRNFLFNG+DELGSKRFLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGEMLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDK G
Sbjct: 301 AGKSYEHRPWDKTG 314

BLAST of Lsi06G005530 vs. ExPASy TrEMBL
Match: A0A0A0LXG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G616870 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 4.0e-134
Identity = 244/311 (78.46%), Postives = 269/311 (86.50%), Query Frame = 0

Query: 4   SSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPSPLQ 63
           SSRL FSLSFN PPFS+NCSSKN+ KRAF PISSISYQKFVQFALDETKLHTHLFPSPLQ
Sbjct: 3   SSRLCFSLSFNSPPFSTNCSSKNRSKRAFFPISSISYQKFVQFALDETKLHTHLFPSPLQ 62

Query: 64  EKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANI 123
           EK+NSM+SLD+K KLEMLSF APKIRLLRSLNIESET+QVLDFAAFPEPEFD+PIFCANI
Sbjct: 63  EKHNSMLSLDQKSKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANI 122

Query: 124 FTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAYERV 183
           FTAA+SSIVVLDLNPLHDVI+QR YREKYYKSLLHLGTKYAE+LP    LT     +   
Sbjct: 123 FTAANSSIVVLDLNPLHDVISQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSP 182

Query: 184 VEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAE 243
           + I+      +         F++  +AWLEL+NN  EETIP +LM+NREAQHRYL WRAE
Sbjct: 183 IVIWSKFSSTESKYDILFSAFMDYYKAWLELMNNTGEETIPPQLMVNREAQHRYLSWRAE 242

Query: 244 KDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGK 303
           KDPGH LLRKLIGETLAKD+VRNFLF+G+DELGSK+FLDYFPEYCCEDGTINTKRSMAGK
Sbjct: 243 KDPGHHLLRKLIGETLAKDVVRNFLFHGVDELGSKKFLDYFPEYCCEDGTINTKRSMAGK 302

Query: 304 SYEHRPWDKRG 306
           SYEHRPWD+RG
Sbjct: 303 SYEHRPWDERG 313

BLAST of Lsi06G005530 vs. ExPASy TrEMBL
Match: A0A1S3BP29 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492001 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.0e-133
Identity = 245/311 (78.78%), Postives = 269/311 (86.50%), Query Frame = 0

Query: 4   SSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPSPLQ 63
           SSRLSFSLSFN PPFS+NC+SKN+ KRA  PISSISYQKFVQFALDETKLHTHLFPSPLQ
Sbjct: 3   SSRLSFSLSFNSPPFSTNCNSKNRFKRACFPISSISYQKFVQFALDETKLHTHLFPSPLQ 62

Query: 64  EKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANI 123
           EK+NSM+SLD+K KLEMLSF APKIRLLRSLNIESET+QVLDFAAFPEPEFD+PIFCANI
Sbjct: 63  EKHNSMLSLDQKAKLEMLSFHAPKIRLLRSLNIESETVQVLDFAAFPEPEFDIPIFCANI 122

Query: 124 FTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYERVVE 183
           FTAA+SSIVVLDLNPLHDVITQR YREKYYKSLLHLGTKYAE+LP    L  ++ +    
Sbjct: 123 FTAANSSIVVLDLNPLHDVITQRYYREKYYKSLLHLGTKYAELLPWGGKLTSESLKFFSP 182

Query: 184 IFL---------SHCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAE 243
           I +          +  +   F++  +AWLEL+NN  EETIPS+LMINREAQHRYL WRAE
Sbjct: 183 IVIWSKFSSTESKYDILSSAFMDYYKAWLELMNNTVEETIPSQLMINREAQHRYLSWRAE 242

Query: 244 KDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGK 303
           KDPGH LLRKLIGETLAKD+V+NFLFNGIDELGSK FLDYFPEY CEDGTINTKRSMAGK
Sbjct: 243 KDPGHHLLRKLIGETLAKDVVQNFLFNGIDELGSKSFLDYFPEYRCEDGTINTKRSMAGK 302

Query: 304 SYEHRPWDKRG 306
           SYEHRPWD+RG
Sbjct: 303 SYEHRPWDERG 313

BLAST of Lsi06G005530 vs. ExPASy TrEMBL
Match: A0A6J1DJE9 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111021651 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 3.2e-131
Identity = 237/315 (75.24%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRA-FIPISSISYQKFVQFALDETKLHTHLFP 60
           M S SRLSF   FN PPFSSNCS KNKC+RA F+P+S+ISYQKFVQFALDETKLHTHL P
Sbjct: 1   MDSCSRLSFCSPFNSPPFSSNCSGKNKCRRASFVPVSAISYQKFVQFALDETKLHTHLCP 60

Query: 61  SPLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIF 120
           +PLQEKYNSMIS+DKK KL+MLSFQAPKIRLLRSLNIESE MQVLDFA FP+PEFDVPIF
Sbjct: 61  TPLQEKYNSMISMDKKAKLQMLSFQAPKIRLLRSLNIESEAMQVLDFAVFPKPEFDVPIF 120

Query: 121 CANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYE 180
           CANIF+AASSSIVVLDLNPLHDVI+QRDY+EKYY+SLLHLGTKYAE+LP    L  ++ +
Sbjct: 121 CANIFSAASSSIVVLDLNPLHDVISQRDYKEKYYESLLHLGTKYAELLPWGGKLTSESLK 180

Query: 181 RVVEIFL---------SHCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLL 240
               I +          +  +   F++  +AWLEL+NN  EET PSRLM+NREAQHRYL 
Sbjct: 181 FFSPIVIWTKFPSTRSKYDILFSAFVDYYKAWLELMNNAREETNPSRLMVNREAQHRYLS 240

Query: 241 WRAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRS 300
           WR EKDPGHGLL++LIGE LAKDL+RNFLFNG+DELGSKRFL+YFPEYCCEDGTIN KRS
Sbjct: 241 WRVEKDPGHGLLKRLIGEALAKDLLRNFLFNGVDELGSKRFLEYFPEYCCEDGTINKKRS 300

Query: 301 MAGKSYEHRPWDKRG 306
           M GKSYEHRPWDKRG
Sbjct: 301 MVGKSYEHRPWDKRG 315

BLAST of Lsi06G005530 vs. NCBI nr
Match: XP_038880083.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Benincasa hispida])

HSP 1 Score: 516.5 bits (1329), Expect = 1.7e-142
Identity = 262/314 (83.44%), Postives = 276/314 (87.90%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           MGSSSRLSFSLSFN PPFS+NCSSKNKCKRAF+PISSISYQKFVQFALDETKLHTHLFPS
Sbjct: 1   MGSSSRLSFSLSFNSPPFSTNCSSKNKCKRAFLPISSISYQKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLD AAFPEPEFDVPIFC
Sbjct: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDLAAFPEPEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAY 180
           ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAE+LP    LT     +
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAELLPWGGKLTSESLKF 180

Query: 181 ERVVEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              + I+      +         F++  +AWLEL++N  EETIPSRL+INREAQHRYL W
Sbjct: 181 FSPIVIWSKFSSTESKYDILFSAFVDYYKAWLELMDNTGEETIPSRLLINREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           R EKDPGHGLLRKLIGE LAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDG+INTKRSM
Sbjct: 241 REEKDPGHGLLRKLIGEMLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGSINTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDK G
Sbjct: 301 AGKSYEHRPWDKTG 314

BLAST of Lsi06G005530 vs. NCBI nr
Match: XP_023515585.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.8 bits (1296), Expect = 1.1e-138
Identity = 252/314 (80.25%), Postives = 273/314 (86.94%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           MGS S L F LSFN PPFSS+CSSKNKC+RAF P+S+ISYQKFVQFALDETKLHTHLFPS
Sbjct: 1   MGSCSTLPFCLSFNSPPFSSSCSSKNKCRRAFFPVSAISYQKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMIS+DKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPE EFDVPIFC
Sbjct: 61  PLQEKYNSMISMDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYER 180
           ANIFTAASSSIVVLDLNPLHDVI QRDYREKYYKS+LHLGTKYAE+LP    L  ++ + 
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSILHLGTKYAELLPWGGKLTSESLKF 180

Query: 181 VVEIFL---------SHCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              I +         ++  +   F++  +AWLELVNN  EETIP RLM+NREAQHRYL W
Sbjct: 181 FSPIVIWNKSSSTESTYDILFSAFVDYYKAWLELVNNAAEETIPFRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGHGLL+KLIGETLAKDLVRNFLFNG+DELGSKRFLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGETLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDKRG
Sbjct: 301 AGKSYEHRPWDKRG 314

BLAST of Lsi06G005530 vs. NCBI nr
Match: XP_022960639.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 495.4 bits (1274), Expect = 4.0e-136
Identity = 250/314 (79.62%), Postives = 270/314 (85.99%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           M S S LSF LSFN P  SS+CSSKNKC+ AFIP+S+ISY+KFVQFALDETKLHTHLFPS
Sbjct: 1   MASCSTLSFCLSFNSPLVSSSCSSKNKCRTAFIPVSAISYRKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMIS+DKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPE EFDVPIFC
Sbjct: 61  PLQEKYNSMISMDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAY 180
           ANIFTAASSSIVVLDLNPLHDVI QRDYREKYYKSL+HLGTKYAE+LP    LT     +
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSLMHLGTKYAELLPWGGKLTSESLKF 180

Query: 181 ERVVEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              + I+      +         F++  +AWLELV+N  EETIPSRLM+NREAQHRYL W
Sbjct: 181 FSPIVIWTKSSSTESTYDVLFSAFVDYYKAWLELVHNAAEETIPSRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGHGLL+KLIGETLAKDLVRNFLFNG+DELGSKRFLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGETLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDKRG
Sbjct: 301 AGKSYEHRPWDKRG 314

BLAST of Lsi06G005530 vs. NCBI nr
Match: KAG6590181.1 (Phytochromobilin:ferredoxin oxidoreductase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 495.0 bits (1273), Expect = 5.2e-136
Identity = 250/314 (79.62%), Postives = 270/314 (85.99%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           M S S LSF LSFN P FSS+CSSKNKC+ AFIP+S+ISY+KFVQFALDETKLHTHLFPS
Sbjct: 1   MASCSTLSFCLSFNSPLFSSSCSSKNKCRTAFIPVSAISYRKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMIS+DKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAF E EFDVPIFC
Sbjct: 61  PLQEKYNSMISMDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFSETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAY 180
           ANIFTAASSSIVVLDLNPLHDVI QRDYREKYYKSL+HLGTKYAE+LP    LT     +
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSLMHLGTKYAELLPWGGKLTSESLKF 180

Query: 181 ERVVEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              + I+      +         F++  +AWLELV+N  EETIPSRLM+NREAQHRYL W
Sbjct: 181 FSPIVIWTKSSSTESTYDVLFSAFVDYYKAWLELVHNAAEETIPSRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGHGLL+KLIGETLAKDLVRNFLFNG+DELGSKRFLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGETLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDKRG
Sbjct: 301 AGKSYEHRPWDKRG 314

BLAST of Lsi06G005530 vs. NCBI nr
Match: XP_022988175.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 493.8 bits (1270), Expect = 1.1e-135
Identity = 249/314 (79.30%), Postives = 267/314 (85.03%), Query Frame = 0

Query: 1   MGSSSRLSFSLSFNFPPFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPS 60
           M S S LSF LSFN  PFSS+CSSKNKC+RAF P+S+ISYQKFVQFALDETKLHTHLFPS
Sbjct: 1   MASCSTLSFCLSFNSTPFSSSCSSKNKCRRAFFPVSAISYQKFVQFALDETKLHTHLFPS 60

Query: 61  PLQEKYNSMISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFC 120
           PLQEKYNSMIS+DK VKL+MLSFQAPKIRLLRSLNIESETMQVLDFAAFPE EFDVPIFC
Sbjct: 61  PLQEKYNSMISMDKNVKLQMLSFQAPKIRLLRSLNIESETMQVLDFAAFPETEFDVPIFC 120

Query: 121 ANIFTAASSSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLP--AALTLGRKAY 180
           ANIFTAASSSIVVLDLNPLHDVI QRDYREKYYKSLLHLG KYAE+LP    LT     +
Sbjct: 121 ANIFTAASSSIVVLDLNPLHDVINQRDYREKYYKSLLHLGIKYAELLPWGGKLTSESLKF 180

Query: 181 ERVVEIFLSHCDMD-------QVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLW 240
              + I+      +         F++  +AWLELVNN  EETIPSRLM+NREAQHRYL W
Sbjct: 181 FSPIVIWTKSSSTESTYDILFSAFVDYYKAWLELVNNAAEETIPSRLMVNREAQHRYLSW 240

Query: 241 RAEKDPGHGLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSM 300
           RAEKDPGHGLL+KLIGE LAKDLVRNFLFNG+DELGSKRFLDYFPEY CEDGT+NTKRSM
Sbjct: 241 RAEKDPGHGLLKKLIGEMLAKDLVRNFLFNGVDELGSKRFLDYFPEYYCEDGTVNTKRSM 300

Query: 301 AGKSYEHRPWDKRG 306
           AGKSYEHRPWDK G
Sbjct: 301 AGKSYEHRPWDKTG 314

BLAST of Lsi06G005530 vs. TAIR 10
Match: AT3G09150.2 (phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synthase (HY2) )

HSP 1 Score: 308.5 bits (789), Expect = 6.4e-84
Identity = 152/298 (51.01%), Postives = 205/298 (68.79%), Query Frame = 0

Query: 17  PFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPSPLQEKYNSMISLDKKV 76
           P   N + + + KR  + +S++SY++F + AL+ET+    L PS LQEKY+SM  LD K 
Sbjct: 28  PNKINFTLRRRKKRFLLRVSAVSYKEFAESALEETRKRIVLEPSHLQEKYSSMTGLDGKT 87

Query: 77  KLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDL 136
           +L+ML+F++ KIRLLRS+ IE+ETMQV DFA F EPE+D PIFCAN FT+ + +IVVLDL
Sbjct: 88  ELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTNVNIVVLDL 147

Query: 137 NPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYERVVEIFL---------S 196
           NPLH +  Q DY++KYY  ++ +  KYAE  P    L  ++ +    + +          
Sbjct: 148 NPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWTRFSSSKEK 207

Query: 197 HCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIG 256
           H  +   FL   QAWLE+   + EE  PS +  N EAQH+YL WRA+KDPGHGLL++L+G
Sbjct: 208 HKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGHGLLKRLVG 267

Query: 257 ETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDKRG 306
           E  AK+L+R+FLFNG+DELG+K F+DYFPEY  EDGT++ KRS+ GKSYE RPWD  G
Sbjct: 268 EAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETRPWDLTG 325

BLAST of Lsi06G005530 vs. TAIR 10
Match: AT3G09150.1 (phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synthase (HY2) )

HSP 1 Score: 299.7 bits (766), Expect = 3.0e-81
Identity = 150/298 (50.34%), Postives = 203/298 (68.12%), Query Frame = 0

Query: 17  PFSSNCSSKNKCKRAFIPISSISYQKFVQFALDETKLHTHLFPSPLQEKYNSMISLDKKV 76
           P   N + + + KR  + +S++SY++F + AL+ET+    L PS LQ  Y+SM  LD K 
Sbjct: 28  PNKINFTLRRRKKRFLLRVSAVSYKEFAESALEETRKRIVLEPSHLQ--YSSMTGLDGKT 87

Query: 77  KLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANIFTAASSSIVVLDL 136
           +L+ML+F++ KIRLLRS+ IE+ETMQV DFA F EPE+D PIFCAN FT+ + +IVVLDL
Sbjct: 88  ELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTNVNIVVLDL 147

Query: 137 NPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYERVVEIFL---------S 196
           NPLH +  Q DY++KYY  ++ +  KYAE  P    L  ++ +    + +          
Sbjct: 148 NPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWTRFSSSKEK 207

Query: 197 HCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGHGLLRKLIG 256
           H  +   FL   QAWLE+   + EE  PS +  N EAQH+YL WRA+KDPGHGLL++L+G
Sbjct: 208 HKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGHGLLKRLVG 267

Query: 257 ETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGKSYEHRPWDKRG 306
           E  AK+L+R+FLFNG+DELG+K F+DYFPEY  EDGT++ KRS+ GKSYE RPWD  G
Sbjct: 268 EAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETRPWDLTG 323

BLAST of Lsi06G005530 vs. TAIR 10
Match: AT3G09150.3 (phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synthase (HY2) )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 131/246 (53.25%), Postives = 171/246 (69.51%), Query Frame = 0

Query: 69  MISLDKKVKLEMLSFQAPKIRLLRSLNIESETMQVLDFAAFPEPEFDVPIFCANIFTAAS 128
           M  LD K +L+ML+F++ KIRLLRS+ IE+ETMQV DFA F EPE+D PIFCAN FT+ +
Sbjct: 1   MTGLDGKTELQMLAFKSSKIRLLRSMAIENETMQVFDFAGFMEPEYDTPIFCANFFTSTN 60

Query: 129 SSIVVLDLNPLHDVITQRDYREKYYKSLLHLGTKYAEVLPAALTLGRKAYERVVEIFL-- 188
            +IVVLDLNPLH +  Q DY++KYY  ++ +  KYAE  P    L  ++ +    + +  
Sbjct: 61  VNIVVLDLNPLHQLTDQTDYQDKYYNKIMSIYHKYAETFPWGGKLTGESIKFFSPLVMWT 120

Query: 189 -------SHCDMDQVFLNRVQAWLELVNNIEEETIPSRLMINREAQHRYLLWRAEKDPGH 248
                   H  +   FL   QAWLE+   + EE  PS +  N EAQH+YL WRA+KDPGH
Sbjct: 121 RFSSSKEKHKALFSAFLEYYQAWLEMTIQVREEMEPSHVRANCEAQHKYLTWRAQKDPGH 180

Query: 249 GLLRKLIGETLAKDLVRNFLFNGIDELGSKRFLDYFPEYCCEDGTINTKRSMAGKSYEHR 306
           GLL++L+GE  AK+L+R+FLFNG+DELG+K F+DYFPEY  EDGT++ KRS+ GKSYE R
Sbjct: 181 GLLKRLVGEAKAKELLRDFLFNGVDELGTKTFIDYFPEYQTEDGTVSDKRSIIGKSYETR 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SR439.0e-8351.01Phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Arabidopsis thalian... [more]
Q10UW63.5e-1826.47Phycoerythrobilin:ferredoxin oxidoreductase OS=Trichodesmium erythraeum (strain ... [more]
Q93TM86.6e-1725.21Phycoerythrobilin:ferredoxin oxidoreductase OS=Nostoc punctiforme (strain ATCC 2... [more]
Q7NL656.1e-1527.31Phycoerythrobilin:ferredoxin oxidoreductase OS=Gloeobacter violaceus (strain ATC... [more]
Q9K4U66.2e-0726.0415,16-dihydrobiliverdin:ferredoxin oxidoreductase OS=Prochlorococcus marinus (st... [more]
Match NameE-valueIdentityDescription
A0A6J1HBM11.9e-13679.62phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita moschata ... [more]
A0A6J1JIV15.6e-13679.30phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucurbita maxima OX... [more]
A0A0A0LXG34.0e-13478.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G616870 PE=3 SV=1[more]
A0A1S3BP292.0e-13378.78phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Cucumis melo OX=365... [more]
A0A6J1DJE93.2e-13175.24phytochromobilin:ferredoxin oxidoreductase, chloroplastic OS=Momordica charantia... [more]
Match NameE-valueIdentityDescription
XP_038880083.11.7e-14283.44phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Benincasa hispida][more]
XP_023515585.11.1e-13880.25phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita pepo subsp.... [more]
XP_022960639.14.0e-13679.62phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita moschata][more]
KAG6590181.15.2e-13679.62Phytochromobilin:ferredoxin oxidoreductase, chloroplastic, partial [Cucurbita ar... [more]
XP_022988175.11.1e-13579.30phytochromobilin:ferredoxin oxidoreductase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G09150.26.4e-8451.01phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synth... [more]
AT3G09150.13.0e-8150.34phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synth... [more]
AT3G09150.31.1e-7253.25phytochromobilin:ferredoxin oxidoreductase, chloroplast / phytochromobilin synth... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.1500.20coord: 34..263
e-value: 4.3E-48
score: 166.0
NoneNo IPR availablePANTHERPTHR34557:SF1PHYTOCHROMOBILIN:FERREDOXIN OXIDOREDUCTASE, CHLOROPLASTICcoord: 18..310
IPR009249Ferredoxin-dependent bilin reductasePFAMPF05996Fe_bilin_redcoord: 51..261
e-value: 4.5E-37
score: 128.0
IPR009249Ferredoxin-dependent bilin reductasePANTHERPTHR34557PHYTOCHROMOBILIN:FERREDOXIN OXIDOREDUCTASE, CHLOROPLASTICcoord: 18..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G005530.1Lsi06G005530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010019 chloroplast-nucleus signaling pathway
biological_process GO:0010024 phytochromobilin biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0050897 cobalt ion binding
molecular_function GO:0050619 phytochromobilin:ferredoxin oxidoreductase activity
molecular_function GO:0016636 oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor