Lsi06G005400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G005400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionTHUMP domain-containing protein 1 homolog
Locationchr06: 6696015 .. 6700919 (-)
RNA-Seq ExpressionLsi06G005400
SyntenyLsi06G005400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGAGAACAAACCCTCCACCGCTGGCAAGAAGAGGAAGCAGCGTTTTCTCCCCCACAACGTTACCTCTCAATCTCTTCTTCTTCTTCTTCTTCTGTCTTCCTCTATTCTATTGTCAGTTAATTCAATTTCTTCTGATTTTCTTGTTTTTTTCCTTCCTCAGAAACCAGTGAAGAAGAAGGGTCATTACCCATTACGCCCAGGAGTTCAAGGTTTCTTTATCACCTGCGATGGCGGCCGAGAACGCCAAGCTTCCGTTGAAGCCATCAACGTTATTGACTCTGTATGACTTTCCCCCTTTTTTCTGTTTCTGGGTTATGATTTGGTGGGGTTCAGATGAATGTTCGAATGTATACCGTTTGATTGTCACTAAGTCAGTCGGGGAATTGGAAATTTTATTTTTGCCTTGTTAGAAATAGCAGAGATTAAAAGAGGTGAAATGGCAGAGAGAGGATTAATTTAAGATAATTATAGAGAGAGTTGGGGGGAGGTAATGTAAAGCGTGGTACTCAATACTCAAAGGTTTTCAAATGCAAAGAGCCTGTTTCTATATGCTAGACCAAAACATATTTGTGAGCAGTGAATACTAATCCACTGATATCTATCATGGATGTAAAGGGAAAGTGTTCAAGCTTATCACAAAAGTAGCTCTGGTTTTTTTGTCAATTTTTCGTCTAATTTGTTCATTGTAGGGTTATAAATTGCTACTTCTTGAAATGCTATATTAATATATATTTTTCTTTTAAAGGCTTTACTGTAGTTATGTTTACAAGAGTGTTGGATTAAAACGGAGTTCTTTTCTTCCGTTATTCAATTTTCAGTTTTTTGACGAGTTAGCCCATGGAAAGAATTGTGGTGTGAAGCTGACAGAATTAGCTGATAAACCTTTAAACAAAAAAATTAAGTTTACAGATTCTGAGTCTTCTAGTTCTGATGATGATAATGAGGAGGAGGAGGAGGAGGAGAAGGGGGATGAGGATGAGAATAAGAAGGTTCCTGAGGAAGAGTCTAAGTCTCCCAAGGAGCAAAATGATCCTTCTAATGAAAATGAAACAACTGACGTTCTTAATCCTCGTCAAGGGGACACTGTAGCTTCTGAAAATCAAACAGAGGAAAAATTGAATGACAATGAAAGAGATATGAAGGGTCCTGAAGTTCCATCAGAGGAAGTTGAGGAGCCACCAATTAAGAAGCACTGCATTGAAGCAAATGCATCAAAAACTATTATAAAGGAAAAAGAGAAGTCCATTGATCAACTGATTGAGGCTGAACTTGAGGAATTGGGAGATAAGAGTAAGGTCAGTGATAAAATTTCAATGTCAAGGTTATTTTCATTTCTAATCTTTCATAATTTTTTGTTATTATGACAAGCATGTCCCTGCACGATGACATGAAAATTGTATTATATTGATGGGATTTTGAGTAGGTTTTTTAAACAGGCCTGCTCCAATTTTGATCAACTTTGCAGGTCTTAATACTTTAAGAAAGAATAAGAGCGGACTACTTTCTTTGTTAAATGACTATCTGATTTAAGATTGTCGAGTTCATTGCGCCTTCTTAATCTGACAAGTGACAACAGTCTGCAAGCCGTATGAAATGGGTTTAATTCGTGGACCAAGTATTCTTAGTCTCTATTGATAGAATATGATGCTCGTATTTTTTTATGATAAATATAACAATTTCTCTTCATTTTAATACTTTTCTGTCTTTCTTCTTCTTATGCGTTATTGGGTTAAACCTTTCGATATTGTATTATAAATAATCTTGCAATTGATAACTTTTTATGTGTTATGATTATCACTTGCATTGCGTGCTAGTATTTTTCAACATATTTGTGATATTGCATTGTTTTCTTGAGTAATTTTATAGCATACAGTATTATTAGTTATCCGGACTGGTAGCTGATCCTTTGTTATCCAATCTTCTTATTATGGAATGTGATACAGAGACGTTTTTTCAATCTTGATTCGGGATGTAATGGTGTTGTCTTTATTCAAATGCGGAAGAGTGATGGAGAACCTGGTCCTGAGGCTGTTGTGCAGCATATGATGACATCTATTGCTTCAACAAGGAAACACATATCAAGGTTTGCCTAATTGTTGAATTTCTGTAGGGAACAATGCATGAAAATGTTTATATACTGGATCACTAAAATGCTTTGTATGTTTGATATAATTCAGGTTCATACTTCGAGTACTGCCAATTGAAGTGGCATGCTATGCTTCAGTTGAGGAGATCACGAGGGCAATAAAGCCTCTAATAGAAAAGAACTTTCCTGTTGAAAGTCAAAGTCCTGTAAAGGTATATTCCTTATATATTCCCTTTTCAATATCATGGTGCCTCTTCCTTTGTGATAATCCCCTATATTCAACGCAAAAGAGCAGGAAAGAAATGTTTAACAATGATCTCCTGGAAAACGTGTTAATGAAGATTGAGGAAAAATTAGAGATTCAAATTTCAATGACTATTTGAATAGAACCATTTCAATTTCAACACTACCATGAATTGGAAATAGTTGTGCAGATCTCTTGATGACAGTATTTTTCTGAAATTGAGGATGAGTGTTCCAGATTTGCTTTCTTGATTTATGGTCAGTTTTGTGACTGAGTTCTAGGTCTCATGTTCAATTCACGTGTACAGATAACTAAAAGTGTCATTGGGTTAACGAAGATTAGGTTGTGTATGCTTATAAATGTTGAAAGTTGCTAATGTAGTTGTCAACCAGGTAGAAGATAAGGAGAATCTATGTTTAGTTATCTTATATTGGCCGAAACTGAAAGCTTTTAAAGTAGTTTTGGTTGGTTAGTTCTACACAATTTTTACTATTTTAAAATATTATCTTCAGTGAAGTCTTAACAATGATCTATTATTAATTATAGAAATGTTTTATGGATTTAAATTACTTTTCATCTTTCCTTGTTGCAAACTCGTTGACAAATGCATTATTTTTTTTTCTTTTTCCTCTTTTTAATAATACTTCAAAATAACTTTGTAAAACAAAATATGTCATGTGTTAGTTTTTAAATGTTTGTAGTTTCAATTAATCTGACCCAACACATTTTGACACTTCCTAGTTGTATGATTAAATTGCAACTTCCAGTCAGTAAAGGACATGGGAGAATGCATTTAAGTGACTATAATAAACTATTTATTGATTTATAAAAGAAACTAGCATCCTATATAAATATTTTACAAGAAGTGAATATTTATGATTAATCTCGTTTCCTATGAGATGATTATCGATCTATGCAATGAATCCAATGAATTCCTTTTTTTTTTTTCCAGGGTTTCACTGTGATTTGACATGCTGATTCTGTTATTAGGATGCTCACAAATTATTCTTTAAATTTTTATGAACTTTCTTGCAGTTTGCAGTATTATATGAGGCCCGAGCAAATACTGGCATTGACAGGGCAACTATAATTAATACAGTAGCAAAATCTGTTCCTGAGCCTCATAAAGTTGATTTGAACAATCCTGAGAAAACCATTGTGGTTGAGATTGTCAAGGTGAGGATCTCTGTTCTTGGAAGTAAATAATCTTGGAGATTTTCTCTTTAGACATGATTCAGTTAATTGAATGTTTGCCATGGCCACCCACTCGGTGAGGATGTGTGCATATTCAAATTATCTGTTCAGAATATTGTGGTCAGAGAGGCTCTCTAACCGACCTACCTTGCTTTGTGGATTTAATCTAACTTTGGTTTCAAACTTGACTTCTTTAACCAGACTTCTTTAACCAAAAATGATTTCTTTAACATATATCAGTTTATTGAATGTTGCCATGGCCACCCGCTCAGTGAGGGTGTGTGCAGCTTCAAATTATCTGTTCAAAATATTGTGGTCAGAGAGGCTCTAACCTACCTTGCTTTGTGGATTAAATCTAATCTTGGTTTCAAACTTGACTTCGTTAACCAAAAATTGATTTTTTTAAGAAAAATGTTTGCTTTGAACTTTGAAGTTGAGTCTTCTGGAACTGAAGTTTATATTTTTACCCTTACCCGAATGAAAGGAAAGTGTTGTAGAACCCCTCTATCTTGCAGAACTGGCCTTATCTGCTGGGTTGACTGGGAGGCATTAACTATGGCCTTAGAACATGAAGTATTGCTGAACCACAAGTTTAGAACTTGTTTGGTGAGACTGGATGCCAGTTAGTAGGCTGAATCAGATTGAACTTGACAGCCCATGGCTATAAAAAGTGCAAAAAGAAAAAGTAAATAACCATCTTTGATCATTGATCATCATACATTTCTGGTAGGCTGAATCAGATTTAATTTGACAACCCATGGTTATAAGTAAATAACAATCTTTGATCTTCTTTCTTTCTTTCTTTTTTTCTTTCTTTTTTAATTCTTTTTCTGAGGTAGCCAACAGAGTTACTTTCTATTCAGTGTTGCATATTCTACAGTTGTTTTCCTGATTTGTGAATTATTGTGCTCTTGTGATTGCTCTGGGACTTAGACGATAGGCTCTCTAATTCTTCGAGAAGAAGTTTCTTACTTATTGTTTGTTCTTCAGACAATTTGCTTGATTGGAGTGGTGGAGAAGTATAAGGAGTTGTCCAAGTACAATCTGAGGCAGCTTACATCAAGAAAGCAATAGGATAGGTTTTTTATTTGTTTTTCTCGTCTGTTTCAGCTTTGGGCACTTTTTAATGTTCAAATGTATTCAGCACATTTAGAAAATTTTGGAACCAAACAATTATATTATATAGTTGTTGGCATATAACAAAATCTGATAGCTTTAGGTGTTTCTGGAAACTTGTCTCTAAGCAATTTGGCCTGGTCCCTGGAAGAAATCAAACTGGTCTTACGCTCTCGATCATTTGAATTTTTAGTTTAGGTCTTATTTTCGTCATTTCTTTTCAAAAATGGAAAAAAGTATTGAAGTTGGCTTGACTC

mRNA sequence

ATGGCCGCCGAGAACAAACCCTCCACCGCTGGCAAGAAGAGGAAGCAGCGTTTTCTCCCCCACAACGTTACCTCTCAATCTCTTCTTCTTCTTCTTCTTCTGTCTTCCTCTATTCTATTGTCAGTTAATTCAATTTCTTCTGATTTTCTTGTTTTTTTCCTTCCTCAGAAACCAGTGAAGAAGAAGGGTCATTACCCATTACGCCCAGGAGTTCAAGGTTTCTTTATCACCTGCGATGGCGGCCGAGAACGCCAAGCTTCCGTTGAAGCCATCAACGTTATTGACTCTTTTTTTGACGAGTTAGCCCATGGAAAGAATTGTGGTGTGAAGCTGACAGAATTAGCTGATAAACCTTTAAACAAAAAAATTAAGTTTACAGATTCTGAGTCTTCTAGTTCTGATGATGATAATGAGGAGGAGGAGGAGGAGGAGAAGGGGGATGAGGATGAGAATAAGAAGGTTCCTGAGGAAGAGTCTAAGTCTCCCAAGGAGCAAAATGATCCTTCTAATGAAAATGAAACAACTGACGTTCTTAATCCTCGTCAAGGGGACACTGTAGCTTCTGAAAATCAAACAGAGGAAAAATTGAATGACAATGAAAGAGATATGAAGGGTCCTGAAGTTCCATCAGAGGAAGTTGAGGAGCCACCAATTAAGAAGCACTGCATTGAAGCAAATGCATCAAAAACTATTATAAAGGAAAAAGAGAAGTCCATTGATCAACTGATTGAGGCTGAACTTGAGGAATTGGGAGATAAGAGTAAGAGACGTTTTTTCAATCTTGATTCGGGATGTAATGGTGTTGTCTTTATTCAAATGCGGAAGAGTGATGGAGAACCTGGTCCTGAGGCTGTTGTGCAGCATATGATGACATCTATTGCTTCAACAAGGAAACACATATCAAGGTTCATACTTCGAGTACTGCCAATTGAAGTGGCATGCTATGCTTCAGTTGAGGAGATCACGAGGGCAATAAAGCCTCTAATAGAAAAGAACTTTCCTGTTGAAAGTCAAAGTCCTGTAAAGTTTGCAGTATTATATGAGGCCCGAGCAAATACTGGCATTGACAGGGCAACTATAATTAATACAGTAGCAAAATCTGTTCCTGAGCCTCATAAAGTTGATTTGAACAATCCTGAGAAAACCATTGTGGTTGAGATTGTCAAGACAATTTGCTTGATTGGAGTGGTGGAGAAGTATAAGGAGTTGTCCAAGTACAATCTGAGGCAGCTTACATCAAGAAAGCAATAGGATAGGTTTTTTATTTGTTTTTCTCGTCTGTTTCAGCTTTGGGCACTTTTTAATGTTCAAATGTATTCAGCACATTTAGAAAATTTTGGAACCAAACAATTATATTATATAGTTGTTGGCATATAACAAAATCTGATAGCTTTAGGTGTTTCTGGAAACTTGTCTCTAAGCAATTTGGCCTGGTCCCTGGAAGAAATCAAACTGGTCTTACGCTCTCGATCATTTGAATTTTTAGTTTAGGTCTTATTTTCGTCATTTCTTTTCAAAAATGGAAAAAAGTATTGAAGTTGGCTTGACTC

Coding sequence (CDS)

ATGGCCGCCGAGAACAAACCCTCCACCGCTGGCAAGAAGAGGAAGCAGCGTTTTCTCCCCCACAACGTTACCTCTCAATCTCTTCTTCTTCTTCTTCTTCTGTCTTCCTCTATTCTATTGTCAGTTAATTCAATTTCTTCTGATTTTCTTGTTTTTTTCCTTCCTCAGAAACCAGTGAAGAAGAAGGGTCATTACCCATTACGCCCAGGAGTTCAAGGTTTCTTTATCACCTGCGATGGCGGCCGAGAACGCCAAGCTTCCGTTGAAGCCATCAACGTTATTGACTCTTTTTTTGACGAGTTAGCCCATGGAAAGAATTGTGGTGTGAAGCTGACAGAATTAGCTGATAAACCTTTAAACAAAAAAATTAAGTTTACAGATTCTGAGTCTTCTAGTTCTGATGATGATAATGAGGAGGAGGAGGAGGAGGAGAAGGGGGATGAGGATGAGAATAAGAAGGTTCCTGAGGAAGAGTCTAAGTCTCCCAAGGAGCAAAATGATCCTTCTAATGAAAATGAAACAACTGACGTTCTTAATCCTCGTCAAGGGGACACTGTAGCTTCTGAAAATCAAACAGAGGAAAAATTGAATGACAATGAAAGAGATATGAAGGGTCCTGAAGTTCCATCAGAGGAAGTTGAGGAGCCACCAATTAAGAAGCACTGCATTGAAGCAAATGCATCAAAAACTATTATAAAGGAAAAAGAGAAGTCCATTGATCAACTGATTGAGGCTGAACTTGAGGAATTGGGAGATAAGAGTAAGAGACGTTTTTTCAATCTTGATTCGGGATGTAATGGTGTTGTCTTTATTCAAATGCGGAAGAGTGATGGAGAACCTGGTCCTGAGGCTGTTGTGCAGCATATGATGACATCTATTGCTTCAACAAGGAAACACATATCAAGGTTCATACTTCGAGTACTGCCAATTGAAGTGGCATGCTATGCTTCAGTTGAGGAGATCACGAGGGCAATAAAGCCTCTAATAGAAAAGAACTTTCCTGTTGAAAGTCAAAGTCCTGTAAAGTTTGCAGTATTATATGAGGCCCGAGCAAATACTGGCATTGACAGGGCAACTATAATTAATACAGTAGCAAAATCTGTTCCTGAGCCTCATAAAGTTGATTTGAACAATCCTGAGAAAACCATTGTGGTTGAGATTGTCAAGACAATTTGCTTGATTGGAGTGGTGGAGAAGTATAAGGAGTTGTCCAAGTACAATCTGAGGCAGCTTACATCAAGAAAGCAATAG

Protein sequence

MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVKKKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLNKKIKFTDSESSSSDDDNEEEEEEEKGDEDENKKVPEEESKSPKEQNDPSNENETTDVLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ
Homology
BLAST of Lsi06G005400 vs. ExPASy Swiss-Prot
Match: Q9VZD8 (THUMP domain-containing protein 1 homolog OS=Drosophila melanogaster OX=7227 GN=CG15014 PE=1 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 7.2e-21
Identity = 61/172 (35.47%), Postives = 101/172 (58.72%), Query Frame = 0

Query: 245 AELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHISRFI 304
           A+  E+  + K RF N+D+     VFI+ +  D    P A+ +H++  IA+T K +SRF+
Sbjct: 111 AKCREMLSQRKMRFQNVDTNTTNCVFIRTQLED----PVALGKHIINDIATTGKSMSRFV 170

Query: 305 LRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATIINTV 364
           LR++PIEV C A++ +I  A   L +K+F  E   P  + +++  R N  I R  II  +
Sbjct: 171 LRLVPIEVVCRANMPDIITAAGELFDKHFLKE---PTSYGIIFNHRYNQQIKRDQIITQL 230

Query: 365 AKSVPEP---HKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTS 414
           A+ V      +KVDL   +K+I+VE+++  CL+ V++ Y E  K+NL +L +
Sbjct: 231 AELVNSKNVGNKVDLKEAKKSIIVEVLRGWCLLSVIDNYLECKKFNLAELAN 275

BLAST of Lsi06G005400 vs. ExPASy Swiss-Prot
Match: Q24K03 (THUMP domain-containing protein 1 OS=Bos taurus OX=9913 GN=THUMPD1 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.0e-19
Identity = 70/219 (31.96%), Postives = 122/219 (55.71%), Query Frame = 0

Query: 196 LNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSIDQLIEAELEELGDKSK 255
           LN+   DM GPE  +++ ++P   +   E +  +  +K++   I    E  L        
Sbjct: 65  LNEYGDDMYGPEKFADKDQQPSGSEG--EDDDVEAALKKEVGDIKASTEMRL-------- 124

Query: 256 RRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHISRFILRVLPIEVACY 315
           RRF +++SG N VVFI+    +    PE +V H++  I  T+K  +R ILR+LPI   C 
Sbjct: 125 RRFQSVESGANNVVFIRTLGIE----PEKLVHHILQDIYKTKKKKTRVILRMLPISGTCK 184

Query: 316 ASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATIINTVA---KSVPEPH 375
           A +E++ +  +  +E  F   ++    F ++Y++R N+ ++R  +I  +A    S+   +
Sbjct: 185 AFLEDMKKYAETFLEPWFKAPNKG--TFQIVYKSRNNSHMNREEVIKELAGIVGSLNSEN 244

Query: 376 KVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQL 412
           KVDL+NP+ T+VVEI+K +C + VV+ Y    KYNL+++
Sbjct: 245 KVDLSNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEV 267

BLAST of Lsi06G005400 vs. ExPASy Swiss-Prot
Match: Q9NXG2 (THUMP domain-containing protein 1 OS=Homo sapiens OX=9606 GN=THUMPD1 PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 5.1e-19
Identity = 68/219 (31.05%), Postives = 122/219 (55.71%), Query Frame = 0

Query: 196 LNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSIDQLIEAELEELGDKSK 255
           LN+   DM GPE  +++ ++P   +   E + ++  +K++   I    E  L        
Sbjct: 65  LNEYGDDMYGPEKFTDKDQQPSGSEG--EDDDAEAALKKEVGDIKASTEMRL-------- 124

Query: 256 RRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHISRFILRVLPIEVACY 315
           RRF +++SG N VVFI+    +    PE +V H++  +  T+K  +R ILR+LPI   C 
Sbjct: 125 RRFQSVESGANNVVFIRTLGIE----PEKLVHHILQDMYKTKKKKTRVILRMLPISGTCK 184

Query: 316 ASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATIINTVAK---SVPEPH 375
           A +E++ +  +  +E  F   ++    F ++Y++R N+ ++R  +I  +A    ++   +
Sbjct: 185 AFLEDMKKYAETFLEPWFKAPNKG--TFQIVYKSRNNSHVNREEVIRELAGIVCTLNSEN 244

Query: 376 KVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQL 412
           KVDL NP+ T+VVEI+K +C + VV+ Y    KYNL+++
Sbjct: 245 KVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEV 267

BLAST of Lsi06G005400 vs. ExPASy Swiss-Prot
Match: Q99J36 (THUMP domain-containing protein 1 OS=Mus musculus OX=10090 GN=Thumpd1 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.2e-15
Identity = 79/273 (28.94%), Postives = 139/273 (50.92%), Query Frame = 0

Query: 155 PEEESKSPKEQNDPSNENETTDVLNPRQGD-----TVASENQTEEK--------LNDNER 214
           P    +  K Q  P+      D   PRQ +      + + N  E K        LN+   
Sbjct: 11  PVAGKRKGKSQFLPAKRARRGDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGD 70

Query: 215 DMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSIDQLIEAELEELGDKSKRRFFNL 274
           DM GPE   ++ ++P         + S+    + E ++ + +  +++   +K  RRF ++
Sbjct: 71  DMYGPEKFIDKDQQP---------SGSEGEDDDAEAALKKEV-GDIKASTEKRLRRFQSV 130

Query: 275 DSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHISRFILRVLPIEVACYASVEEI 334
           +SG N VVFI+    +    PE +V H++  +  T+K  +R ILR+LPI   C A +E++
Sbjct: 131 ESGANNVVFIRTLGIE----PEKLVHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDM 190

Query: 335 TRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATIINTVA---KSVPEPHKVDLNN 394
            +  +  +E  F   ++    F ++Y++R N+ ++R  +I  +A    S+   +KVDL N
Sbjct: 191 KKYAETFLEPWFKAPNKG--TFQIVYKSRNNSHMNREEVIKELAGIVGSLNSENKVDLTN 250

Query: 395 PEKTIVVEIVKTICLIGVVEKYKELSKYNLRQL 412
           PE T+VVEI+K +C + VV+ Y    KYNL+++
Sbjct: 251 PEYTVVVEIIKAVCCLSVVKDYVLFRKYNLQEV 267

BLAST of Lsi06G005400 vs. ExPASy Swiss-Prot
Match: P87151 (Uncharacterized protein C25H2.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC25H2.10c PE=4 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 9.7e-10
Identity = 51/191 (26.70%), Postives = 94/191 (49.21%), Query Frame = 0

Query: 224 EANASKTIIKEKEKSIDQLIEAELEELGDKSKRRFFN---LDSGCNGVVFIQMRKSDGEP 283
           E  +S    +E+ + ++  +  E+  L  K+K+       LD  C  V F++ R     P
Sbjct: 69  ETQSSDQENEEEGEELEDAVAKEINSLQKKNKKELLTPIMLDMPC--VYFVKTR-----P 128

Query: 284 GPEAVVQHMMTSIASTRKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSP 343
             + V     T      K ++R+  R++PI      S++++    K LI+  F    +  
Sbjct: 129 PIDPVRLVEFTCEVGKTKKMTRYTQRLIPIVRTTGVSLDDLEELAKSLIDPLFHEGQEGI 188

Query: 344 VKFAVLYEARANTGIDRATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKY 403
            +FAV    R +T + +  I  TVA+ V + H VDL N +  I+V+++K I  I +V+ +
Sbjct: 189 KEFAVQANIRNHTVLKKDDIYRTVARIVGKQHMVDLKNFKLLILVQVIKNIIGISIVQNF 248

Query: 404 KELSKYNLRQL 412
           +EL ++NL ++
Sbjct: 249 EELRRFNLNEV 252

BLAST of Lsi06G005400 vs. ExPASy TrEMBL
Match: A0A1S3BP64 (LOW QUALITY PROTEIN: THUMP domain-containing protein 1 homolog OS=Cucumis melo OX=3656 GN=LOC103492015 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.9e-183
Identity = 348/416 (83.65%), Postives = 368/416 (88.46%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAA+NKPS  GKKRKQRFLPHN                                  KPVK
Sbjct: 1   MAADNKPSAGGKKRKQRFLPHN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDELAHGKNCGVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNEEEEEEEKGDEDENKKVPEEESKSPKEQNDPSNENETTDVLNP 180
           KKIKF+DSESSSSDDD+ EEEEEEKGD+DENKKVPEE SKSPKEQN+PSNENETTDV+NP
Sbjct: 121 KKIKFSDSESSSSDDDDNEEEEEEKGDDDENKKVPEEGSKSPKEQNEPSNENETTDVVNP 180

Query: 181 RQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSID 240
            Q +TVA +NQ EEK NDNERDMK PEVPSEEVEEPP KKHC+EA+ASK+ IKEKEKSID
Sbjct: 181 NQEETVALKNQKEEKSNDNERDMKCPEVPSEEVEEPPNKKHCVEADASKSNIKEKEKSID 240

Query: 241 QLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHI 300
           QLIEAEL+ELGDK+KRRFFNLDSGCNGVVFIQMRK+DGEPGP+AVVQHMMTSIASTRKHI
Sbjct: 241 QLIEAELQELGDKTKRRFFNLDSGCNGVVFIQMRKNDGEPGPKAVVQHMMTSIASTRKHI 300

Query: 301 SRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATI 360
           SRFILR+LPIEVACYASVEEITRAIKPLIEKNFP+ESQ+ VKFAVLYEARANTGIDRATI
Sbjct: 301 SRFILRILPIEVACYASVEEITRAIKPLIEKNFPIESQNAVKFAVLYEARANTGIDRATI 360

Query: 361 INTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 417
           INTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKE SKYNLRQLTSRKQ
Sbjct: 361 INTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKEFSKYNLRQLTSRKQ 382

BLAST of Lsi06G005400 vs. ExPASy TrEMBL
Match: A0A6J1HUY9 (THUMP domain-containing protein 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111467052 PE=4 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.4e-176
Identity = 345/420 (82.14%), Postives = 367/420 (87.38%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAAENKPSTAGKKRKQR+ P+N                                  KPVK
Sbjct: 1   MAAENKPSTAGKKRKQRYRPNN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDEL HGK  GVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELVHGKGSGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNEEEEEEEKGD--EDENKKVPEEE--SKSPKEQNDPSNENETTD 180
           KKIKF+DSESS SDDD+ EEEEEE GD  EDENK+VP+EE  SKSPKEQNDP NENETTD
Sbjct: 121 KKIKFSDSESSGSDDDDGEEEEEEVGDENEDENKEVPDEESKSKSPKEQNDPCNENETTD 180

Query: 181 VLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKE 240
           V +P QGDTVA+E++TEEK NDNE+D+K PEV SEEVEEPP KKHCIEA+ASK+I+KEKE
Sbjct: 181 VPDPHQGDTVATESRTEEKSNDNEKDIKCPEVRSEEVEEPPKKKHCIEADASKSIVKEKE 240

Query: 241 KSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIAST 300
           KSIDQLIEAEL+ELGDKSKRRFFNLDSGCNGVVFIQMRK+DGEPGPEAVVQHMMTSIAST
Sbjct: 241 KSIDQLIEAELQELGDKSKRRFFNLDSGCNGVVFIQMRKNDGEPGPEAVVQHMMTSIAST 300

Query: 301 RKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGID 360
           RKHISRFILRVLP+EVACYASVEEI+RAIKPLIEKNFPVESQSP KFAVLYEAR+NTGID
Sbjct: 301 RKHISRFILRVLPVEVACYASVEEISRAIKPLIEKNFPVESQSPKKFAVLYEARSNTGID 360

Query: 361 RATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 417
           RATIINTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ
Sbjct: 361 RATIINTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 386

BLAST of Lsi06G005400 vs. ExPASy TrEMBL
Match: A0A6J1EQU7 (THUMP domain-containing protein 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111436964 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.3e-176
Identity = 345/421 (81.95%), Postives = 368/421 (87.41%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAAENKPSTAGKKRKQR+ P+N                                  KPVK
Sbjct: 1   MAAENKPSTAGKKRKQRYRPNN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDEL HGK  GVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELVHGKGSGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNE-EEEEEEKG--DEDENKKVPEEE--SKSPKEQNDPSNENETT 180
           KKIKF+DSESS SDDD + EEEEEE G  +EDENK+VP+EE  SKSPKEQNDP NENETT
Sbjct: 121 KKIKFSDSESSGSDDDGDGEEEEEEVGGENEDENKEVPDEESKSKSPKEQNDPCNENETT 180

Query: 181 DVLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEK 240
           DV +P QGDTVA+E++TEEK NDNE+D+K PEVPSEEVEEPP KKHCIEA+ASK+I+KEK
Sbjct: 181 DVPDPHQGDTVATESRTEEKSNDNEKDIKCPEVPSEEVEEPPKKKHCIEADASKSIVKEK 240

Query: 241 EKSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIAS 300
           EKSIDQLIEAEL+ELGDKSKRRFFNLDSGCNGVVFIQMRK+DGEPGPEAVVQHMMTSIAS
Sbjct: 241 EKSIDQLIEAELQELGDKSKRRFFNLDSGCNGVVFIQMRKNDGEPGPEAVVQHMMTSIAS 300

Query: 301 TRKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGI 360
           TRKHISRFILRVLP+EVACYASVEEI+RAIKPLIEKNFPVESQSP KFAVLYEAR+NTGI
Sbjct: 301 TRKHISRFILRVLPVEVACYASVEEISRAIKPLIEKNFPVESQSPKKFAVLYEARSNTGI 360

Query: 361 DRATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK 417
           DRATIINTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK
Sbjct: 361 DRATIINTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK 387

BLAST of Lsi06G005400 vs. ExPASy TrEMBL
Match: A0A6J1DK01 (THUMP domain-containing protein 1 OS=Momordica charantia OX=3673 GN=LOC111021610 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 9.7e-162
Identity = 322/418 (77.03%), Postives = 348/418 (83.25%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAAENKPST  KKRKQR+LPHN                                  KPVK
Sbjct: 1   MAAENKPSTGAKKRKQRYLPHN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPL PGVQGFF+TCDGGRERQAS+EAINVIDSFF+EL HGK  GVKL++LADKPLN
Sbjct: 61  KKGHYPLHPGVQGFFLTCDGGRERQASIEAINVIDSFFEELVHGKVSGVKLSQLADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNEEEEEEEKGDEDENKKVPEEE--SKSPKEQNDPSNENETTDVL 180
           KKIKF+DSESSSSDDDNE+E     GDE+E ++VPEEE  SKS  EQNDPSNENETT+ +
Sbjct: 121 KKIKFSDSESSSSDDDNEDE-----GDEEE-EEVPEEEGKSKSHTEQNDPSNENETTEKV 180

Query: 181 NPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKS 240
           +P QGD V +ENQTEEK NDN  D+K  EV S EVEEPP KKHCIEA+ SK +++EK KS
Sbjct: 181 DPHQGDAVVTENQTEEKSNDNS-DVKCHEVLSNEVEEPPSKKHCIEADTSKNVVQEKAKS 240

Query: 241 IDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRK 300
           IDQLIEAELEELGDK+KRR FNLDSGCNGVVFIQMRK DGEPGPE VV+HMMTSIASTRK
Sbjct: 241 IDQLIEAELEELGDKNKRRLFNLDSGCNGVVFIQMRKGDGEPGPEDVVKHMMTSIASTRK 300

Query: 301 HISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRA 360
           HISRFILRVLPIEVACYASVEEI+RAIKPLIEKNFPVES+SPVKFAVLYEARAN+GIDRA
Sbjct: 301 HISRFILRVLPIEVACYASVEEISRAIKPLIEKNFPVESESPVKFAVLYEARANSGIDRA 360

Query: 361 TIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 417
           TIINTVAK VPEPHKVDLNNPEKTIVVEIVKTICLIGVVE+YKELSKYNLRQLTS KQ
Sbjct: 361 TIINTVAKCVPEPHKVDLNNPEKTIVVEIVKTICLIGVVERYKELSKYNLRQLTSGKQ 377

BLAST of Lsi06G005400 vs. ExPASy TrEMBL
Match: A0A5A7U5T9 (THUMP domain-containing protein 1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1250G00540 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.9e-142
Identity = 277/342 (80.99%), Postives = 295/342 (86.26%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAA+NKPS  GKKRKQRFLPHN                                  KPVK
Sbjct: 1   MAADNKPSAGGKKRKQRFLPHN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDELAHGKNCGVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNEEEEEEEKGDEDENKKVPEEESKSPKEQNDPSNENETTDVLNP 180
           KKIKF+DSESSSSDDD+ EEEEEEKGD+DENKKVPEE SKSPKEQN+PSNENETTDV+NP
Sbjct: 121 KKIKFSDSESSSSDDDDNEEEEEEKGDDDENKKVPEEGSKSPKEQNEPSNENETTDVVNP 180

Query: 181 RQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSID 240
            Q +TVA ENQ EEK NDNERDMK PEVPSEEVEEPP KKHC+EA+ASK+ IKEKEKSID
Sbjct: 181 NQEETVAPENQKEEKSNDNERDMKCPEVPSEEVEEPPNKKHCVEADASKSNIKEKEKSID 240

Query: 241 QLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHI 300
           QLIEAEL+ELGDK+KRRFFNLDSGCNGVVFIQMRK+DGEPGP+AVVQHMMTSIASTRKHI
Sbjct: 241 QLIEAELQELGDKTKRRFFNLDSGCNGVVFIQMRKNDGEPGPKAVVQHMMTSIASTRKHI 300

Query: 301 SRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVK 343
           SRFILR+LPIEVACYASVEEITRAIKPLIEKNFP+ESQ+ VK
Sbjct: 301 SRFILRILPIEVACYASVEEITRAIKPLIEKNFPIESQNAVK 308

BLAST of Lsi06G005400 vs. NCBI nr
Match: XP_004139043.1 (THUMP domain-containing protein 1 homolog [Cucumis sativus] >KAE8653515.1 hypothetical protein Csa_007492 [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 4.9e-184
Identity = 355/420 (84.52%), Postives = 373/420 (88.81%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAA+NKPS  GKKRKQRFLPHN                                  KPVK
Sbjct: 1   MAADNKPSAGGKKRKQRFLPHN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDELA GKNCGVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELALGKNCGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNE----EEEEEEKGDEDENKKVPEEESKSPKEQNDPSNENETTD 180
           KKIKF+DSESSSSDDD++    EEEEEEKGD+DENKKVPEEESKSPKEQN+PSNENETTD
Sbjct: 121 KKIKFSDSESSSSDDDDDDNDNEEEEEEKGDDDENKKVPEEESKSPKEQNEPSNENETTD 180

Query: 181 VLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKE 240
           V NP Q +TVASENQTEEK N+NERDMK PEVPSEEVEEPP KKHCIEA+ASK+IIKEKE
Sbjct: 181 VANPHQEETVASENQTEEKSNENERDMKCPEVPSEEVEEPPNKKHCIEADASKSIIKEKE 240

Query: 241 KSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIAST 300
           KSIDQLIEAEL+ELGDK+KRRFFNLDSGCNGVVFIQMRK+DGEPGPEAVVQHMMTSIAST
Sbjct: 241 KSIDQLIEAELQELGDKTKRRFFNLDSGCNGVVFIQMRKNDGEPGPEAVVQHMMTSIAST 300

Query: 301 RKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGID 360
           RKHISRFILR+LPIEVACYASVEEITRAIKPLIEKNFPVESQ+PVKFAVLYEARAN+GID
Sbjct: 301 RKHISRFILRILPIEVACYASVEEITRAIKPLIEKNFPVESQNPVKFAVLYEARANSGID 360

Query: 361 RATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 417
           RATIINTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ
Sbjct: 361 RATIINTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 386

BLAST of Lsi06G005400 vs. NCBI nr
Match: XP_008450398.1 (PREDICTED: LOW QUALITY PROTEIN: THUMP domain-containing protein 1 homolog [Cucumis melo])

HSP 1 Score: 649.4 bits (1674), Expect = 2.1e-182
Identity = 348/416 (83.65%), Postives = 368/416 (88.46%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAA+NKPS  GKKRKQRFLPHN                                  KPVK
Sbjct: 1   MAADNKPSAGGKKRKQRFLPHN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDELAHGKNCGVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNEEEEEEEKGDEDENKKVPEEESKSPKEQNDPSNENETTDVLNP 180
           KKIKF+DSESSSSDDD+ EEEEEEKGD+DENKKVPEE SKSPKEQN+PSNENETTDV+NP
Sbjct: 121 KKIKFSDSESSSSDDDDNEEEEEEKGDDDENKKVPEEGSKSPKEQNEPSNENETTDVVNP 180

Query: 181 RQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKEKSID 240
            Q +TVA +NQ EEK NDNERDMK PEVPSEEVEEPP KKHC+EA+ASK+ IKEKEKSID
Sbjct: 181 NQEETVALKNQKEEKSNDNERDMKCPEVPSEEVEEPPNKKHCVEADASKSNIKEKEKSID 240

Query: 241 QLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIASTRKHI 300
           QLIEAEL+ELGDK+KRRFFNLDSGCNGVVFIQMRK+DGEPGP+AVVQHMMTSIASTRKHI
Sbjct: 241 QLIEAELQELGDKTKRRFFNLDSGCNGVVFIQMRKNDGEPGPKAVVQHMMTSIASTRKHI 300

Query: 301 SRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGIDRATI 360
           SRFILR+LPIEVACYASVEEITRAIKPLIEKNFP+ESQ+ VKFAVLYEARANTGIDRATI
Sbjct: 301 SRFILRILPIEVACYASVEEITRAIKPLIEKNFPIESQNAVKFAVLYEARANTGIDRATI 360

Query: 361 INTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 417
           INTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKE SKYNLRQLTSRKQ
Sbjct: 361 INTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKEFSKYNLRQLTSRKQ 382

BLAST of Lsi06G005400 vs. NCBI nr
Match: XP_022967605.1 (THUMP domain-containing protein 1 homolog [Cucurbita maxima])

HSP 1 Score: 629.0 bits (1621), Expect = 2.9e-176
Identity = 345/420 (82.14%), Postives = 367/420 (87.38%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAAENKPSTAGKKRKQR+ P+N                                  KPVK
Sbjct: 1   MAAENKPSTAGKKRKQRYRPNN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDEL HGK  GVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELVHGKGSGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNEEEEEEEKGD--EDENKKVPEEE--SKSPKEQNDPSNENETTD 180
           KKIKF+DSESS SDDD+ EEEEEE GD  EDENK+VP+EE  SKSPKEQNDP NENETTD
Sbjct: 121 KKIKFSDSESSGSDDDDGEEEEEEVGDENEDENKEVPDEESKSKSPKEQNDPCNENETTD 180

Query: 181 VLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEKE 240
           V +P QGDTVA+E++TEEK NDNE+D+K PEV SEEVEEPP KKHCIEA+ASK+I+KEKE
Sbjct: 181 VPDPHQGDTVATESRTEEKSNDNEKDIKCPEVRSEEVEEPPKKKHCIEADASKSIVKEKE 240

Query: 241 KSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIAST 300
           KSIDQLIEAEL+ELGDKSKRRFFNLDSGCNGVVFIQMRK+DGEPGPEAVVQHMMTSIAST
Sbjct: 241 KSIDQLIEAELQELGDKSKRRFFNLDSGCNGVVFIQMRKNDGEPGPEAVVQHMMTSIAST 300

Query: 301 RKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGID 360
           RKHISRFILRVLP+EVACYASVEEI+RAIKPLIEKNFPVESQSP KFAVLYEAR+NTGID
Sbjct: 301 RKHISRFILRVLPVEVACYASVEEISRAIKPLIEKNFPVESQSPKKFAVLYEARSNTGID 360

Query: 361 RATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 417
           RATIINTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ
Sbjct: 361 RATIINTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRKQ 386

BLAST of Lsi06G005400 vs. NCBI nr
Match: KAG6588199.1 (THUMP domain-containing protein 1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 627.9 bits (1618), Expect = 6.4e-176
Identity = 345/421 (81.95%), Postives = 369/421 (87.65%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAAENKPSTAGKKRKQR+ P+N                                  KPVK
Sbjct: 1   MAAENKPSTAGKKRKQRYRPNN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDEL HGK  GVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELVHGKGSGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNE-EEEEEEKGDE--DENKKVPEEE--SKSPKEQNDPSNENETT 180
           KKIKF+DSESS SDDD++ EEEEEE GDE  DENK+VP+EE  SKSPKEQNDP NENETT
Sbjct: 121 KKIKFSDSESSGSDDDDDGEEEEEEVGDENKDENKEVPDEESKSKSPKEQNDPCNENETT 180

Query: 181 DVLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEK 240
           DV +P QGDTVA+E++TEEK NDNE+D+K PEVPSEEVEEPP KKHCIEA+ASK+I+KEK
Sbjct: 181 DVPDPHQGDTVATESRTEEKSNDNEKDIKCPEVPSEEVEEPPKKKHCIEADASKSIVKEK 240

Query: 241 EKSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIAS 300
           EKSIDQLIEAEL+ELGDKSKRRFFNLDSGCNGVVFIQMRK+DGEPGPEAVVQH+MTSIAS
Sbjct: 241 EKSIDQLIEAELQELGDKSKRRFFNLDSGCNGVVFIQMRKNDGEPGPEAVVQHIMTSIAS 300

Query: 301 TRKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGI 360
           TRKHISRFILRVLP+EVACYASVEEI+RAIKPLIEKNFPVESQSP KFAVLYEAR+NTGI
Sbjct: 301 TRKHISRFILRVLPVEVACYASVEEISRAIKPLIEKNFPVESQSPKKFAVLYEARSNTGI 360

Query: 361 DRATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK 417
           DRATIINTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK
Sbjct: 361 DRATIINTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK 387

BLAST of Lsi06G005400 vs. NCBI nr
Match: XP_022930547.1 (THUMP domain-containing protein 1 homolog [Cucurbita moschata])

HSP 1 Score: 627.1 bits (1616), Expect = 1.1e-175
Identity = 345/421 (81.95%), Postives = 368/421 (87.41%), Query Frame = 0

Query: 1   MAAENKPSTAGKKRKQRFLPHNVTSQSLLLLLLLSSSILLSVNSISSDFLVFFLPQKPVK 60
           MAAENKPSTAGKKRKQR+ P+N                                  KPVK
Sbjct: 1   MAAENKPSTAGKKRKQRYRPNN----------------------------------KPVK 60

Query: 61  KKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKLTELADKPLN 120
           KKGHYPLRPGVQGFFITCDGGRERQAS+EAINVIDSFFDEL HGK  GVKLTELADKPLN
Sbjct: 61  KKGHYPLRPGVQGFFITCDGGRERQASIEAINVIDSFFDELVHGKGSGVKLTELADKPLN 120

Query: 121 KKIKFTDSESSSSDDDNE-EEEEEEKG--DEDENKKVPEEE--SKSPKEQNDPSNENETT 180
           KKIKF+DSESS SDDD + EEEEEE G  +EDENK+VP+EE  SKSPKEQNDP NENETT
Sbjct: 121 KKIKFSDSESSGSDDDGDGEEEEEEVGGENEDENKEVPDEESKSKSPKEQNDPCNENETT 180

Query: 181 DVLNPRQGDTVASENQTEEKLNDNERDMKGPEVPSEEVEEPPIKKHCIEANASKTIIKEK 240
           DV +P QGDTVA+E++TEEK NDNE+D+K PEVPSEEVEEPP KKHCIEA+ASK+I+KEK
Sbjct: 181 DVPDPHQGDTVATESRTEEKSNDNEKDIKCPEVPSEEVEEPPKKKHCIEADASKSIVKEK 240

Query: 241 EKSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGPEAVVQHMMTSIAS 300
           EKSIDQLIEAEL+ELGDKSKRRFFNLDSGCNGVVFIQMRK+DGEPGPEAVVQHMMTSIAS
Sbjct: 241 EKSIDQLIEAELQELGDKSKRRFFNLDSGCNGVVFIQMRKNDGEPGPEAVVQHMMTSIAS 300

Query: 301 TRKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVKFAVLYEARANTGI 360
           TRKHISRFILRVLP+EVACYASVEEI+RAIKPLIEKNFPVESQSP KFAVLYEAR+NTGI
Sbjct: 301 TRKHISRFILRVLPVEVACYASVEEISRAIKPLIEKNFPVESQSPKKFAVLYEARSNTGI 360

Query: 361 DRATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK 417
           DRATIINTVAK+VPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK
Sbjct: 361 DRATIINTVAKAVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKELSKYNLRQLTSRK 387

BLAST of Lsi06G005400 vs. TAIR 10
Match: AT5G12410.1 (THUMP domain-containing protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.5e-101
Identity = 219/371 (59.03%), Postives = 276/371 (74.39%), Query Frame = 0

Query: 53  FLPQ-KPVKKKGHYPLRPGVQGFFITCDGGRERQASVEAINVIDSFFDELAHGKNCGVKL 112
           + PQ +PVKKKG YPL+PGVQGFFI+CDGGRE QA+ EAINVIDSFF+EL  G +  V  
Sbjct: 14  YRPQNRPVKKKGSYPLKPGVQGFFISCDGGREFQAAQEAINVIDSFFEELIQGTDSKVN- 73

Query: 113 TELADKPLNKKIKFTDSESSSSDDDNEEEEEEEKGDEDENKKVPEEESKSPKEQNDPSNE 172
             L   P+NKK+ F     S S+D++EEE+E   G+E+ENK   ++   S +  ND  NE
Sbjct: 74  PGLFGNPINKKVTF-----SYSEDEDEEEDESNNGEEEENKGDGDKAVVS-EGGNDLVNE 133

Query: 173 NE-----TTDVLNPRQGDTVASENQTEEK-LNDNERDMKGPEVPSEE--VEEPPIKKHCI 232
            E       D +N ++   +ASE   E K L +NE   +  +  +++   +EPP KK C 
Sbjct: 134 KEIASEGVNDQVNEKE---IASEGSCEVKQLAENETVKEEEDKGNQKNGGDEPPRKKTCT 193

Query: 233 -EANASKTIIKEKEKSIDQLIEAELEELGDKSKRRFFNLDSGCNGVVFIQMRKSDGEPGP 292
            EAN    + +  EKSID+LIEAEL+ELGDKSKRRF  LD GCNG+VFIQM+K DG+P P
Sbjct: 194 EEANPLAKVNENAEKSIDKLIEAELKELGDKSKRRFMKLDPGCNGLVFIQMKKRDGDPSP 253

Query: 293 EAVVQHMMTSIASTRKHISRFILRVLPIEVACYASVEEITRAIKPLIEKNFPVESQSPVK 352
           + +VQH MTS A+T+KH+SRFILR+LPIEV+CY S EEI+RAIKPL+E+ FP+E+++P K
Sbjct: 254 KEIVQHAMTSAAATKKHMSRFILRLLPIEVSCYPSEEEISRAIKPLVEQYFPIETENPRK 313

Query: 353 FAVLYEARANTGIDRATIINTVAKSVPEPHKVDLNNPEKTIVVEIVKTICLIGVVEKYKE 412
           FAVLY ARANTG+DR  IINT+AKS+P PHKVDL+NPE TIVVEI+KT+CLIGVVEKYKE
Sbjct: 314 FAVLYGARANTGLDRMKIINTIAKSIPAPHKVDLSNPEMTIVVEIIKTVCLIGVVEKYKE 373

Query: 413 LSKYNLRQLTS 414
           L+KYNLRQLTS
Sbjct: 374 LAKYNLRQLTS 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9VZD87.2e-2135.47THUMP domain-containing protein 1 homolog OS=Drosophila melanogaster OX=7227 GN=... [more]
Q24K033.0e-1931.96THUMP domain-containing protein 1 OS=Bos taurus OX=9913 GN=THUMPD1 PE=2 SV=1[more]
Q9NXG25.1e-1931.05THUMP domain-containing protein 1 OS=Homo sapiens OX=9606 GN=THUMPD1 PE=1 SV=2[more]
Q99J361.2e-1528.94THUMP domain-containing protein 1 OS=Mus musculus OX=10090 GN=Thumpd1 PE=1 SV=1[more]
P871519.7e-1026.70Uncharacterized protein C25H2.10c OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Match NameE-valueIdentityDescription
A0A1S3BP649.9e-18383.65LOW QUALITY PROTEIN: THUMP domain-containing protein 1 homolog OS=Cucumis melo O... [more]
A0A6J1HUY91.4e-17682.14THUMP domain-containing protein 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1EQU75.3e-17681.95THUMP domain-containing protein 1 homolog OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1DK019.7e-16277.03THUMP domain-containing protein 1 OS=Momordica charantia OX=3673 GN=LOC111021610... [more]
A0A5A7U5T92.9e-14280.99THUMP domain-containing protein 1-like protein OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
XP_004139043.14.9e-18484.52THUMP domain-containing protein 1 homolog [Cucumis sativus] >KAE8653515.1 hypoth... [more]
XP_008450398.12.1e-18283.65PREDICTED: LOW QUALITY PROTEIN: THUMP domain-containing protein 1 homolog [Cucum... [more]
XP_022967605.12.9e-17682.14THUMP domain-containing protein 1 homolog [Cucurbita maxima][more]
KAG6588199.16.4e-17681.95THUMP domain-containing protein 1-like protein, partial [Cucurbita argyrosperma ... [more]
XP_022930547.11.1e-17581.95THUMP domain-containing protein 1 homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G12410.11.5e-10159.03THUMP domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004114THUMP domainSMARTSM00981THUMP_a_2coord: 306..398
e-value: 1.9E-7
score: 40.7
IPR004114THUMP domainPFAMPF02926THUMPcoord: 297..397
e-value: 4.3E-15
score: 56.1
IPR004114THUMP domainPROSITEPS51165THUMPcoord: 292..398
score: 19.496969
NoneNo IPR availableGENE3D3.30.2300.10THUMP superfamilycoord: 315..398
e-value: 8.6E-25
score: 88.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 171..190
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..221
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..221
NoneNo IPR availablePANTHERPTHR13452:SF10THUMP DOMAIN-CONTAINING PROTEIN 1coord: 1..414
NoneNo IPR availableSUPERFAMILY143437THUMP domain-likecoord: 316..397
IPR040183THUMP domain-containing protein 1-likePANTHERPTHR13452THUMP DOMAIN CONTAINING PROTEIN 1-RELATEDcoord: 1..414
IPR040183THUMP domain-containing protein 1-likeCDDcd11717THUMP_THUMPD1_likecoord: 256..398
e-value: 4.39106E-43
score: 146.575

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G005400.1Lsi06G005400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
molecular_function GO:0003723 RNA binding