Lsi06G004990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G004990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionbeta-glucosidase 12-like
Locationchr06: 5974360 .. 5978027 (+)
RNA-Seq ExpressionLsi06G004990
SyntenyLsi06G004990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGGATTCAAAGATGATACCTTCTTTCTTGGCTATCTTTTCCTCATCCTTTTCATAGTTTCAGAGGCTGCCACAAATGAACCAAGCTCTCCAATTCCCATTATAAGGAGAAGCAACTTCTCTAAAGACCTTGTTTTTGGTTCTTCATCTGCTGCTTATCAGGTATATATAATAACAAGTTTTCCTCAGTGTTTCATTCTCCAAATTCATAACACAAATTAAACTCATCAATGCATGCATAACTCTGGTTGAGTTCTAACTTTTTTTCTCTTCCATAGTATGAAGGTGCTGCTTTTAAGTATGGGAGAGGACCAAGTATTTGGGATACCTACACTCACAACCACTCTGGTCTCTCTCTCTAAGGAAATAATATTTGTTTCTCTCTCTCGAAGTAAATAATATTTGTTTGGTGAAATTGCAGAGAGGATTGCTGATGGCAAAAATGGAGATAAGGCTGTTGATCAATACCATCGCTACAAGGTTTGTAGGATGCTTATAGATAAAATAAGAAATCATTGACATTGATATTTTAAACATTTATATATACCACAATTTACATCGGGCACATTTTTTAAATAACTCTCAAACATGGTTTTTAAGAATTCAAAATCAATTTTGATCATGATATAAAAATTGTATTTAAAAATATAAAATAAAATATTAAATTAATTTTGAGTGATTAATGATGTGTATTTTGAAGTGATTTTGAATATAATAAAAGTGATTTTAACAAAACATACACTTCAACTTCCATACCACAATTTGTTTTTATAATTACAGAAAACAAAAACTACAATAACTACAATTATTTGTATATTATTATTCCTCCAAACATCTTAACATGCCTTTGAATATTTAATAAGGTAAGATATCCAAATCTCCAATTCATTCATATATACACATACACATACATTTCTCTAAGTTTTTTTTTTTATTTAATAAAATATTATATATACAAAATTGATTAACAATTTTTCATGTATAATGCAGGAAGATGTTGTCATTATGAACCATATAGGTTTTGATGCTTACCGATTCTCAATTTCCTGGTCTAGAGTCTTACCAAGTAAGGATTGATATTTTAATTATATCACTCGACATATGTAAAAGGTTTACCTTCAAATCTTATATTTCTAGTTCTAAAAAATTATTAAATTAAAAATTCAAGAATGAACATATTTTTACCGATCTAAATATAGTTTAACTGGTTCAAACATATATAAGGTTATTCAAAATTCAAATGTTCCATTAGCATGAACTAAAAAATAAGAACACTTTTTTCAAATATAATCTTTTGATATTTCTTTTTGTTAATTATGATTTTACAAAGTCAAAGATTTTACAACAAAGACTTTATTATAAGGATAAGTTGATGAATGATTTAGTGATATTATATTATATTTTATAAAACTAGATTTTAGAAGTAAAAAGTTTGACTTTCATCTCTTCTGATAATCTTAATTTAAGCTTTTGAATAATTAGATTTTTCATAATTTTAATTTAATTGATAATTTACGTATTATATTGAGTTTTTTAACACTTTAAATATATTAAGAGGGAAAATTTAGTGGTGGTGTGAATAAAGAAGGAATTGACTACTACAACAGACTCATCAACGAACTCCTTTCAAAAGATTGGTATATTATCTTAATACCAAGTTGATTCACTAAAATACTTTTGTGTAATTAATTAATTTTACAATTATAAATTTGTAGGCATCCAATCTTATGTAACAATTTTCCACTGGGATGTTCCTCAAGCTTTACAAGATAAATATCAAGGCTTCTTGGGAAAAGAAATTATGTGAGTAACCTTTTTATGAAAATTGTTATATTCCTCACTATAAAACGAAACATCAACATTATAATATGAATTACTAATTTTCTATTACAAAATATTTTCCCTAGTATTAAAATGAATCTTTTTTTAAAAAAAAAAATCACTGTCATAATATATTACAAATTTTAAAAAAGAAACATGTGTTTCAAGGAAAAGAAATGAAATAGTGTGAAGATTAATTACAGAAATGATTATCGAGACTTTGCCAATCTTTGCTTCAAAGAGTTTGGAGATAGAGTGAAACATTGGATCACTTTTAACGAACAATACGTCTTCACCATAAATGGCTATGCAACGGGGGCCTTTGCACCAGGGAGATGCTCTTCTTGGCAACCATTTAATTGTCTCGGTGGAGATTCTGGGACTGAACCATATCGTGTTGGTCACCATCTAATTCTTGCACACGCTACTGCTTTTAAACTTTACAAGTCCGAATATCAAGTATTTATTCTCCCTCTTATTACACCAACACATGTTTCTTCATGTATATAACCCGACAAGTGAACCTAGGATATGTTGAATTTGATATAGGTTGATTAATTCCGTTCGACCTAAAGCTCAAGATTAAGTTATTGAACTTAAGGTATATTAAACTTTTTAATAAATATATTTTTCAACCATTTTATCGTATGGAATTTGTAGGCACATCAAAAAGGTGAAATTGGGGTGACATTGTTTTCAAATTGGTTTGTCCCATATTCAAGTAGTAATGCAGATAAAGATGCTACATCTCGAGCTCTTGATTTTTCCCTTGGTTGGTAAGTAAAATTAATATAAGTTTTCATTTTAAATTTTGGGTTGTTTTCAATTATAGAAAAATAAACCAAAATATTTATAAATATAGCAAAATTTTATTCTGTATCTGCTATAGACCACAATAGACTACTATCTGTGTCGCAATAGATACAGATAGTAATCCATCGCGGTCTATCACAAATAGACAGTAAAATTTTGTTATATTTGTAAAATAATTTTCCTTGAATTCATTGCACTTTACATTTTGAAAATTACATTATTTTCTCCAAACTATTTGAATGTTATTTTTGTAGGTTCTTGCATCCAATAGTGTATGGAGATTATCCAGCCAGCATGAGAGCTCTTGTGAAGGAAAGATTGCTTAATTTTACAAAGGAAGAAACTAGTTTGATTACAGGGTCCTATGATTTTATTGGAATAAATTACTATACATCTAATTATGCACAAAACAATCCCAATGTTGATCCTCACAAACCAAGTCTCTTGACTGATTTACATGCCAATACTTCAAGTATGAAACTCTATTGCTATCTTTTATTTTAAGATATTACCATTTCTTCTTCATTTTTTTAAAATTGTTCTCATGCAAATAAAAAAAAAAAATTGACTTTGTGAAATGTGCTTTTGGATTGTAGCTGATCGTGATGGAGTCTCAATTGGTCCAAAGGTAATCTATGTGAAAACTTTCCTTCATAGATATATTTAACAATATTTATGTCATTTTCTTTTTATCAAATAATTTGTTACTTATTTATTAATATTAATCTTGTAGGTGAATAGTTCATCTTGGCTTCCTGTTTATCCAGAAGGATTGAAAGATTTGATGATCCATATAAAGGATCATTACAAAAACCCGTTGATCTACATCACAGAAAATGGTACTAAAGGGAATTTTACTTTTTACATGAATAAAAGAAAAAATATTATATTATCTTATTCATTTCGAAAATTTGCATTTTATAGGATATCTCGACTTTGATATTCCCCAAGTTTACAAATTAATCAAGGATGAGGGTAAAGTTAAATACTACCGTCAACATCTCAATAAACTTCACGAATCAATCGGGTGA

mRNA sequence

ATGATGGGATTCAAAGATGATACCTTCTTTCTTGGCTATCTTTTCCTCATCCTTTTCATAGTTTCAGAGGCTGCCACAAATGAACCAAGCTCTCCAATTCCCATTATAAGGAGAAGCAACTTCTCTAAAGACCTTGTTTTTGGTTCTTCATCTGCTGCTTATCAGTATGAAGGTGCTGCTTTTAAGTATGGGAGAGGACCAAGTATTTGGGATACCTACACTCACAACCACTCTGAGAGGATTGCTGATGGCAAAAATGGAGATAAGGCTGTTGATCAATACCATCGCTACAAGGAAGATGTTGTCATTATGAACCATATAGGTTTTGATGCTTACCGATTCTCAATTTCCTGGTCTAGAGTCTTACCAAGCATCCAATCTTATGTAACAATTTTCCACTGGGATGTTCCTCAAGCTTTACAAGATAAATATCAAGGCTTCTTGGGAAAAGAAATTATAAATGATTATCGAGACTTTGCCAATCTTTGCTTCAAAGAGTTTGGAGATAGAGTGAAACATTGGATCACTTTTAACGAACAATACGTCTTCACCATAAATGGCTATGCAACGGGGGCCTTTGCACCAGGGAGATGCTCTTCTTGGCAACCATTTAATTGTCTCGGTGGAGATTCTGGGACTGAACCATATCGTGTTGGTCACCATCTAATTCTTGCACACGCTACTGCTTTTAAACTTTACAAGTCCGAATATCAAGCACATCAAAAAGGTGAAATTGGGGTGACATTGTTTTCAAATTGGTTCTTGCATCCAATAGTGTATGGAGATTATCCAGCCAGCATGAGAGCTCTTGTGAAGGAAAGATTGCTTAATTTTACAAAGGAAGAAACTAGTTTGATTACAGGGTCCTATGATTTTATTGGAATAAATTACTATACATCTAATTATGCACAAAACAATCCCAATGTTGATCCTCACAAACCAAGTCTCTTGACTGATTTACATGCCAATACTTCAACTGATCGTGATGGAGTCTCAATTGGTCCAAAGGTGAATAGTTCATCTTGGCTTCCTGTTTATCCAGAAGGATTGAAAGATTTGATGATCCATATAAAGGATCATTACAAAAACCCGTTGATCTACATCACAGAAAATGGATATCTCGACTTTGATATTCCCCAAGTTTACAAATTAATCAAGGATGAGGGTAAAGTTAAATACTACCGTCAACATCTCAATAAACTTCACGAATCAATCGGGTGA

Coding sequence (CDS)

ATGATGGGATTCAAAGATGATACCTTCTTTCTTGGCTATCTTTTCCTCATCCTTTTCATAGTTTCAGAGGCTGCCACAAATGAACCAAGCTCTCCAATTCCCATTATAAGGAGAAGCAACTTCTCTAAAGACCTTGTTTTTGGTTCTTCATCTGCTGCTTATCAGTATGAAGGTGCTGCTTTTAAGTATGGGAGAGGACCAAGTATTTGGGATACCTACACTCACAACCACTCTGAGAGGATTGCTGATGGCAAAAATGGAGATAAGGCTGTTGATCAATACCATCGCTACAAGGAAGATGTTGTCATTATGAACCATATAGGTTTTGATGCTTACCGATTCTCAATTTCCTGGTCTAGAGTCTTACCAAGCATCCAATCTTATGTAACAATTTTCCACTGGGATGTTCCTCAAGCTTTACAAGATAAATATCAAGGCTTCTTGGGAAAAGAAATTATAAATGATTATCGAGACTTTGCCAATCTTTGCTTCAAAGAGTTTGGAGATAGAGTGAAACATTGGATCACTTTTAACGAACAATACGTCTTCACCATAAATGGCTATGCAACGGGGGCCTTTGCACCAGGGAGATGCTCTTCTTGGCAACCATTTAATTGTCTCGGTGGAGATTCTGGGACTGAACCATATCGTGTTGGTCACCATCTAATTCTTGCACACGCTACTGCTTTTAAACTTTACAAGTCCGAATATCAAGCACATCAAAAAGGTGAAATTGGGGTGACATTGTTTTCAAATTGGTTCTTGCATCCAATAGTGTATGGAGATTATCCAGCCAGCATGAGAGCTCTTGTGAAGGAAAGATTGCTTAATTTTACAAAGGAAGAAACTAGTTTGATTACAGGGTCCTATGATTTTATTGGAATAAATTACTATACATCTAATTATGCACAAAACAATCCCAATGTTGATCCTCACAAACCAAGTCTCTTGACTGATTTACATGCCAATACTTCAACTGATCGTGATGGAGTCTCAATTGGTCCAAAGGTGAATAGTTCATCTTGGCTTCCTGTTTATCCAGAAGGATTGAAAGATTTGATGATCCATATAAAGGATCATTACAAAAACCCGTTGATCTACATCACAGAAAATGGATATCTCGACTTTGATATTCCCCAAGTTTACAAATTAATCAAGGATGAGGGTAAAGTTAAATACTACCGTCAACATCTCAATAAACTTCACGAATCAATCGGGTGA

Protein sequence

MMGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLPSIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSNWFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFDIPQVYKLIKDEGKVKYYRQHLNKLHESIG
Homology
BLAST of Lsi06G004990 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.7e-123
Identity = 218/444 (49.10%), Postives = 291/444 (65.54%), Query Frame = 0

Query: 15  FLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTYT 74
           FL+L +V+  A N    P P+ RRS F K  +FG++S++YQYEG A + GRGPSIWDT+T
Sbjct: 15  FLLLAVVASGAYNSAGEP-PVSRRS-FPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFT 74

Query: 75  HNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLPS---------- 134
           H H E+IAD  NGD A D YH YKEDV +M  +G DAYRFSISW+R+LP+          
Sbjct: 75  HQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKE 134

Query: 135 ----------------IQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEFG 194
                           +Q ++T+FHWD PQAL+DKY GFL   IIND++D+A +CFKEFG
Sbjct: 135 GIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFG 194

Query: 195 DRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHAT 254
           DRVK+WITFNE + F  NGYATG FAPGRCS W+  NC  GDSG EPY   HH +LAHA 
Sbjct: 195 DRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAE 254

Query: 255 AFKLYKSEYQAHQKGEIGVTLFSN------------------------WFLHPIVYGDYP 314
             +LYK++YQA QKG+IG+TL S+                        WF+ P++ GDYP
Sbjct: 255 TVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNNDAAKRAIDFMFGWFMDPLIRGDYP 314

Query: 315 ASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHANT 374
            SMR LV  RL  FTKE++ L+ G++DFIG+NYYT+NYA N P  +    S  TD  AN 
Sbjct: 315 LSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANL 374

Query: 375 STDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFD---IPQV 406
           +  R+G+ IGP+  +S WL VYP+G +DL++++K++Y NP +YITENG  +F+   +P +
Sbjct: 375 TGVRNGIPIGPQA-ASPWLYVYPQGFRDLLLYVKENYGNPTVYITENGVDEFNNKTLP-L 434

BLAST of Lsi06G004990 vs. ExPASy Swiss-Prot
Match: Q7XKV4 (Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2)

HSP 1 Score: 443.4 bits (1139), Expect = 2.9e-123
Identity = 218/444 (49.10%), Postives = 291/444 (65.54%), Query Frame = 0

Query: 15  FLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTYT 74
           FL+L +V+  A N    P P+ RRS F K  +FG++S++YQYEG A + GRGPSIWDT+T
Sbjct: 15  FLLLAVVASGAYNGAGEP-PVSRRS-FPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFT 74

Query: 75  HNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLPS---------- 134
           H H E+IAD  NGD A D YH YKEDV +M  +G DAYRFSISW+R+LP+          
Sbjct: 75  HQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKE 134

Query: 135 ----------------IQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEFG 194
                           +Q ++T+FHWD PQAL+DKY GFL   IIND++D+A +CFKEFG
Sbjct: 135 GIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFG 194

Query: 195 DRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHAT 254
           DRVK+WITFNE + F  NGYATG FAPGRCS W+  NC  GDSG EPY   HH +LAHA 
Sbjct: 195 DRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAE 254

Query: 255 AFKLYKSEYQAHQKGEIGVTLFSN------------------------WFLHPIVYGDYP 314
             +LYK++YQA QKG+IG+TL S+                        WF+ P++ GDYP
Sbjct: 255 TVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIRGDYP 314

Query: 315 ASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHANT 374
            SMR LV  RL  FTKE++ L+ G++DFIG+NYYT+NYA N P  +    S  TD  AN 
Sbjct: 315 LSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANL 374

Query: 375 STDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFD---IPQV 406
           +  R+G+ IGP+  +S WL VYP+G +DL++++K++Y NP +YITENG  +F+   +P +
Sbjct: 375 TGVRNGIPIGPQA-ASPWLYVYPQGFRDLLLYVKENYGNPTVYITENGVDEFNNKTLP-L 434

BLAST of Lsi06G004990 vs. ExPASy Swiss-Prot
Match: Q7XKV2 (Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2)

HSP 1 Score: 424.1 bits (1089), Expect = 1.8e-117
Identity = 218/458 (47.60%), Postives = 289/458 (63.10%), Query Frame = 0

Query: 2   MGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAF 61
           M    +   LG + L L +V  A + EP    P I R +F +  +FG++S++YQYEG A 
Sbjct: 1   MAAAGEVVMLGGILLPLLLV-VAVSGEP----PPISRRSFPEGFIFGTASSSYQYEGGAR 60

Query: 62  KYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRV 121
           + GRGPSIWDT+TH H ++IAD  NGD A D YH YKEDV IM  +G DAYRFSISW+R+
Sbjct: 61  EGGRGPSIWDTFTHQHPDKIADKSNGDVAADSYHLYKEDVRIMKDMGVDAYRFSISWTRI 120

Query: 122 LPS--------------------------IQSYVTIFHWDVPQALQDKYQGFLGKEIIND 181
           LP+                          +Q +VT+FHWD PQAL+DKY GFL   IIND
Sbjct: 121 LPNGSLSGGINREGISYYNNLINELLLKGVQPFVTLFHWDSPQALEDKYNGFLSPNIIND 180

Query: 182 YRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGA-FAPGRCSSWQPFNCLGGDSGTE 241
           Y+++A  CFKEFGDRVKHWITFNE   F + GYA+G  FAPGRCS W+  NC  GDSG E
Sbjct: 181 YKEYAETCFKEFGDRVKHWITFNEPLSFCVAGYASGGMFAPGRCSPWEG-NCSAGDSGRE 240

Query: 242 PYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN---------------------- 301
           PY   HH +LAHA   +LYK +YQ  QKG+IG+TL SN                      
Sbjct: 241 PYTACHHQLLAHAETVRLYKEKYQVLQKGKIGITLVSNWFVPFSRSKSNIDAARRALDFM 300

Query: 302 --WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVD 361
             WF+ P++ G+YP SMR LV+ RL  FTKE++ LI GS+DFIG+NYYTSNYA + P  +
Sbjct: 301 LGWFMDPLIRGEYPLSMRELVRNRLPQFTKEQSELIKGSFDFIGLNYYTSNYAGSLPPSN 360

Query: 362 PHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITE 406
               S  TD  AN +  R+G+ IGP+  +S WL +YP+G ++L++++K++Y NP IYITE
Sbjct: 361 GLNNSYSTDARANLTAVRNGIPIGPQA-ASPWLYIYPQGFRELVLYVKENYGNPTIYITE 420

BLAST of Lsi06G004990 vs. ExPASy Swiss-Prot
Match: Q7XKV5 (Beta-glucosidase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU11 PE=3 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-115
Identity = 203/438 (46.35%), Postives = 273/438 (62.33%), Query Frame = 0

Query: 12  GYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWD 71
           G L L+  +          S +P I R +F K  +FG+SS++YQ+EG A   GRGPSIWD
Sbjct: 11  GALLLLHLLAFTCVACNGGSELPPISRRSFPKGFIFGTSSSSYQFEGGAVLGGRGPSIWD 70

Query: 72  TYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLPS------- 131
           T+TH   ++I D  NGD A D YH YKEDV  M  +G DAYRFSISWSR+LPS       
Sbjct: 71  TFTHQSPDKITDRSNGDVACDSYHLYKEDVRSMKEMGMDAYRFSISWSRILPSALSGGVN 130

Query: 132 ------------------IQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKE 191
                             +Q +VT+FHWD PQAL+DKY+GFL   IINDY+++A  CFKE
Sbjct: 131 REGISYYNNLINELLSKGVQPFVTLFHWDSPQALEDKYKGFLSPNIINDYKEYAETCFKE 190

Query: 192 FGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAH 251
           FGDRVKHWITFNE + F   GYA+G  APGRCSSW+   C  GDSG EPY   HH +LAH
Sbjct: 191 FGDRVKHWITFNEPWTFCSMGYASGIMAPGRCSSWEVGKCRVGDSGREPYTACHHQLLAH 250

Query: 252 ATAFKLYKSEYQAHQKGEIGVTLFSN------------------------WFLHPIVYGD 311
           A   +LYK +YQA QKG+IG+ L ++                        WF+ P++ GD
Sbjct: 251 AETVRLYKEKYQALQKGKIGIILNADWFVPLSQSKSSSDAARRALDFMLGWFMDPLIRGD 310

Query: 312 YPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHA 371
           YP SMR LV  RL  F+KE++ ++ G++DFIG+NYYTS+YA N+P    H  S  TD HA
Sbjct: 311 YPLSMRELVGNRLPEFSKEQSGMVKGAFDFIGLNYYTSSYADNDPPSHGHNNSYNTDAHA 370

Query: 372 NTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGY--LDFDIPQ 399
             +  R+G+ IGP+  +S W  +YPEG+ ++++++K++Y NP IYITENG   ++     
Sbjct: 371 KITGSRNGIPIGPQA-ASFWFHIYPEGICEMLLYVKENYGNPTIYITENGVDEVNNKTMP 430

BLAST of Lsi06G004990 vs. ExPASy Swiss-Prot
Match: P26205 (Cyanogenic beta-glucosidase (Fragment) OS=Trifolium repens OX=3899 GN=LI PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.7e-115
Identity = 197/398 (49.50%), Postives = 264/398 (66.33%), Query Frame = 0

Query: 32  PIPI-------IRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTYTHNHSERIADG 91
           P+PI       + RS F+   VFG++S+A+QYEGAAF+ G+GPSIWDT+TH + E+I D 
Sbjct: 14  PLPISFDDFSDLNRSCFAPGFVFGTASSAFQYEGAAFEDGKGPSIWDTFTHKYPEKIKDR 73

Query: 92  KNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP--------------------- 151
            NGD A+D+YHRYKED+ IM  +  DAYRFSISW RVLP                     
Sbjct: 74  TNGDVAIDEYHRYKEDIGIMKDMNLDAYRFSISWPRVLPKGKLSGGVNREGINYYNNLIN 133

Query: 152 -----SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEFGDRVKHWITFN 211
                 +Q YVT+FHWDVPQAL+D+Y+GFLG+ I++D+RD+A LCFKEFGDRVKHWIT N
Sbjct: 134 EVLANGMQPYVTLFHWDVPQALEDEYRGFLGRNIVDDFRDYAELCFKEFGDRVKHWITLN 193

Query: 212 EQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHATAFKLYKSEYQ 271
           E +  ++N YA G FAPGRCS W   NC GGDSG EPY   H+ +LAHA A +LYK++YQ
Sbjct: 194 EPWGVSMNAYAYGTFAPGRCSDWLKLNCTGGDSGREPYLAAHYQLLAHAAAARLYKTKYQ 253

Query: 272 AHQKGEIGVTLFSN------------------------WFLHPIVYGDYPASMRALVKER 331
           A Q G IG+TL S+                        WF+HP+  G YP SMR LV++R
Sbjct: 254 ASQNGIIGITLVSHWFEPASKEKADVDAAKRGLDFMLGWFMHPLTKGRYPESMRYLVRKR 313

Query: 332 LLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHANTSTDRDGVSIG 373
           L  F+ EE+  +TGS+DF+G+NYY+S YA   P +   +P++ TD   N + + +G  +G
Sbjct: 314 LPKFSTEESKELTGSFDFLGLNYYSSYYAAKAPRIPNARPAIQTDSLINATFEHNGKPLG 373

BLAST of Lsi06G004990 vs. ExPASy TrEMBL
Match: A0A5D3DW45 (Beta-glucosidase 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold93G00040 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.5e-186
Identity = 328/455 (72.09%), Postives = 359/455 (78.90%), Query Frame = 0

Query: 1   MMGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAA 60
           MMGF+  TFFLG +FLI  IVSE A+++PSSPIPI+RRSNF K+ VFGSSS+AYQYEGA 
Sbjct: 1   MMGFR-HTFFLGLVFLISLIVSE-ASHQPSSPIPIVRRSNFPKNFVFGSSSSAYQYEGAV 60

Query: 61  FKYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSR 120
              GR PSIWDTYTH H ERIADGKNGD AVD+YHRYKEDV IM  IGF AYRFSISWSR
Sbjct: 61  ATDGRKPSIWDTYTHKHPERIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSR 120

Query: 121 VLPS--------------------------IQSYVTIFHWDVPQALQDKYQGFLGKEIIN 180
           ++P+                          IQSYVTIFHWDVPQAL+D YQGFL  +IIN
Sbjct: 121 IIPTGKLKDGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIN 180

Query: 181 DYRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTE 240
           DY DFA LCFKEFGDRVKHWITFNEQYVF INGY  GAFAPGRCSSWQPFNC GG+SGTE
Sbjct: 181 DYGDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCTGGNSGTE 240

Query: 241 PYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN---------------------- 300
           PY VGHH IL+HA A K+YKS+YQA QKGEIGVTLFSN                      
Sbjct: 241 PYIVGHHQILSHAVAVKIYKSKYQARQKGEIGVTLFSNWFVPYSNSEADRNATVRALDFQ 300

Query: 301 --WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVD 360
             WFL P+V+GDYPA+M+ALVK+RL  FTKE+  LI GSYDFIGINYYTSNYAQNNPNVD
Sbjct: 301 LGWFLDPVVHGDYPANMKALVKDRLPKFTKEQIKLINGSYDFIGINYYTSNYAQNNPNVD 360

Query: 361 PHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITE 406
           P KPSLLTDL AN+STDR+GVSIGPKVN+SSWL VYPEGLKDLMIHIK+HYKNP +YITE
Sbjct: 361 PSKPSLLTDLRANSSTDRNGVSIGPKVNASSWLAVYPEGLKDLMIHIKNHYKNPNLYITE 420

BLAST of Lsi06G004990 vs. ExPASy TrEMBL
Match: A0A1S3BNM6 (beta-glucosidase 12-like OS=Cucumis melo OX=3656 GN=LOC103492050 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.5e-186
Identity = 328/455 (72.09%), Postives = 359/455 (78.90%), Query Frame = 0

Query: 1   MMGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAA 60
           MMGF+  TFFLG +FLI  IVSE A+++PSSPIPI+RRSNF K+ VFGSSS+AYQYEGA 
Sbjct: 1   MMGFR-HTFFLGLVFLISLIVSE-ASHQPSSPIPIVRRSNFPKNFVFGSSSSAYQYEGAV 60

Query: 61  FKYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSR 120
              GR PSIWDTYTH H ERIADGKNGD AVD+YHRYKEDV IM  IGF AYRFSISWSR
Sbjct: 61  ATDGRKPSIWDTYTHKHPERIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSR 120

Query: 121 VLPS--------------------------IQSYVTIFHWDVPQALQDKYQGFLGKEIIN 180
           ++P+                          IQSYVTIFHWDVPQAL+D YQGFL  +IIN
Sbjct: 121 IIPTGKLKDGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIN 180

Query: 181 DYRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTE 240
           DY DFA LCFKEFGDRVKHWITFNEQYVF INGY  GAFAPGRCSSWQPFNC GG+SGTE
Sbjct: 181 DYGDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCTGGNSGTE 240

Query: 241 PYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN---------------------- 300
           PY VGHH IL+HA A K+YKS+YQA QKGEIGVTLFSN                      
Sbjct: 241 PYIVGHHQILSHAVAVKIYKSKYQARQKGEIGVTLFSNWFVPYSNSEADRNATVRALDFQ 300

Query: 301 --WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVD 360
             WFL P+V+GDYPA+M+ALVK+RL  FTKE+  LI GSYDFIGINYYTSNYAQNNPNVD
Sbjct: 301 LGWFLDPVVHGDYPANMKALVKDRLPKFTKEQIKLINGSYDFIGINYYTSNYAQNNPNVD 360

Query: 361 PHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITE 406
           P KPSLLTDL AN+STDR+GVSIGPKVN+SSWL VYPEGLKDLMIHIK+HYKNP +YITE
Sbjct: 361 PSKPSLLTDLRANSSTDRNGVSIGPKVNASSWLAVYPEGLKDLMIHIKNHYKNPNLYITE 420

BLAST of Lsi06G004990 vs. ExPASy TrEMBL
Match: A0A0A0LXF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G611300 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.4e-180
Identity = 324/454 (71.37%), Postives = 352/454 (77.53%), Query Frame = 0

Query: 2   MGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAF 61
           MGFK  TFFLG +FLI  IVSEAA  +PSSPIPIIR+SNF KD VFGSSS+AYQYEGA  
Sbjct: 1   MGFK-HTFFLGLVFLISLIVSEAA-RQPSSPIPIIRKSNFPKDFVFGSSSSAYQYEGAVD 60

Query: 62  KYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRV 121
             GR P           +RIADGKNGD AVD+YHRYKEDV IM  IGF AYRFSISWSR+
Sbjct: 61  IDGRKP-----------KRIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSRI 120

Query: 122 LP--------------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIIND 181
           LP                           IQSYVTIFHWDVPQAL+D YQGFL  +IIND
Sbjct: 121 LPKGKLIGGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIND 180

Query: 182 YRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEP 241
           Y+DFA LCFKEFGDRVKHWITFNEQYVF INGY  GAFAPGRCSSWQPFNCLGG+SGTEP
Sbjct: 181 YQDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCLGGNSGTEP 240

Query: 242 YRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN----------------------- 301
           Y VGH+ IL+HA A K+YKS+YQAHQKGEIGVTLFSN                       
Sbjct: 241 YIVGHYQILSHAAAVKIYKSKYQAHQKGEIGVTLFSNWFVPYSNSEADRNATVRALDFQL 300

Query: 302 -WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDP 361
            WFL+P+VYGDYPASM+ALVK+RL  FTKEET LI GSYDFIGINYYTSNYAQNNPNVDP
Sbjct: 301 GWFLNPVVYGDYPASMKALVKDRLPKFTKEETKLINGSYDFIGINYYTSNYAQNNPNVDP 360

Query: 362 HKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITEN 406
            KPSLLTDL AN+STDR+GVSIGPKVN+SSWL VYPEGLKDLMIHIK+HYKNP +YITEN
Sbjct: 361 SKPSLLTDLRANSSTDRNGVSIGPKVNASSWLAVYPEGLKDLMIHIKNHYKNPNLYITEN 420

BLAST of Lsi06G004990 vs. ExPASy TrEMBL
Match: A0A6J1JG74 (beta-glucosidase 12-like OS=Cucurbita maxima OX=3661 GN=LOC111485433 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 6.8e-160
Identity = 278/440 (63.18%), Postives = 319/440 (72.50%), Query Frame = 0

Query: 16  LILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTYTH 75
           L++ +VS+AA  EPS PI  +RRS+F K  VFGSSS+AYQYEGAAFKYG+GPSIWDTYTH
Sbjct: 16  LVIVVVSKAAV-EPSHPIDAVRRSSFPKGFVFGSSSSAYQYEGAAFKYGKGPSIWDTYTH 75

Query: 76  NHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP------------ 135
            H ERIADG NGD A D YHRYKED+ IM  IGFDA+RFSISW RV P            
Sbjct: 76  QHPERIADGSNGDIAADSYHRYKEDIAIMKQIGFDAFRFSISWPRVFPKGKLSGGVNMEG 135

Query: 136 --------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEFGD 195
                          I+ YVT+FHWD+PQALQD+YQGF+   +IND+RD+A LCFKEFGD
Sbjct: 136 IKYYNNLINELLAHGIKPYVTLFHWDLPQALQDEYQGFMDHRVINDFRDYAELCFKEFGD 195

Query: 196 RVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHATA 255
           RVKHWITFNEQY+F  NGY  G FAPGRCSSWQPFNCLGGDS TEPY VGH+ ILAHA A
Sbjct: 196 RVKHWITFNEQYIFITNGYVYGQFAPGRCSSWQPFNCLGGDSATEPYIVGHNQILAHAAA 255

Query: 256 FKLYKSEYQAHQKGEIGVTLFSN------------------------WFLHPIVYGDYPA 315
            K+YK++Y+AHQ+GEIGVTLFSN                        WFLHP+VYGDYP 
Sbjct: 256 VKVYKTKYKAHQRGEIGVTLFSNWYVPYSDAKEDKEATSRAFDFSLGWFLHPLVYGDYPT 315

Query: 316 SMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHANTS 375
           SMR  VKERL  FTK+E  LIT SYDFIGINYYT+NYA+ +PN      S + D+ A  S
Sbjct: 316 SMRTFVKERLPKFTKDERILITNSYDFIGINYYTANYAKFDPNATSGNTSYVNDIRATLS 375

Query: 376 TDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFDIPQVYKLI 406
           TDRDGVSIGPKVN+SSWL  YP GLK+LMI+IKD YKNP+IYITENG LDF+   V KL+
Sbjct: 376 TDRDGVSIGPKVNASSWLAAYPVGLKNLMIYIKDVYKNPIIYITENGSLDFNSLNVDKLL 435

BLAST of Lsi06G004990 vs. ExPASy TrEMBL
Match: A0A6J1H8A0 (beta-glucosidase 12-like OS=Cucurbita moschata OX=3662 GN=LOC111461397 PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.5e-159
Identity = 278/454 (61.23%), Postives = 321/454 (70.70%), Query Frame = 0

Query: 2   MGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAF 61
           M  K  +  L    +++ +V+  A  EPS PI  +RRS+F K  VFGSSS+AYQYEGAAF
Sbjct: 1   MASKHTSNLLSLACMLVIVVASKAAVEPSHPIDAVRRSSFPKGFVFGSSSSAYQYEGAAF 60

Query: 62  KYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRV 121
           KYG+GPSIWDTYTH H ERIADG NGD+A D YHRYKEDV IM  IGFDAYRFSISW R+
Sbjct: 61  KYGKGPSIWDTYTHQHPERIADGSNGDRAADSYHRYKEDVAIMKRIGFDAYRFSISWPRL 120

Query: 122 LP--------------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIIND 181
           LP                           I+ YVT+FHWD+PQALQD+YQGF+   IIND
Sbjct: 121 LPKGKLSGGVNTQGIVYYNNLINELLAHGIKPYVTLFHWDLPQALQDEYQGFMDDRIIND 180

Query: 182 YRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEP 241
           +RD+A LCFKEFG++VKHWITFNEQY+F  NGY  G FAPGRCSSWQPFNCLGGDS TEP
Sbjct: 181 FRDYAELCFKEFGNKVKHWITFNEQYIFITNGYVFGQFAPGRCSSWQPFNCLGGDSATEP 240

Query: 242 YRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN----------------------- 301
           Y VGH+ ILAHA A  +YK++YQAHQKGEIGVTLFSN                       
Sbjct: 241 YIVGHNQILAHAAAVNVYKTKYQAHQKGEIGVTLFSNWYVPYSDAKEDKEATSRAFDFSL 300

Query: 302 -WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDP 361
            WFLHP+VYGDYP SMR LVKERL  FT E+  LIT SYDFIGINYYT+NYA+ + N   
Sbjct: 301 GWFLHPLVYGDYPTSMRTLVKERLPKFTNEQRILITNSYDFIGINYYTANYAKFDANATS 360

Query: 362 HKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITEN 406
              S + D+ A  STDRDGVSIGPKVN+SSWL  YP GLK+LMI+IKD YKNP+IYITEN
Sbjct: 361 GNTSYVNDIRATLSTDRDGVSIGPKVNASSWLAAYPVGLKNLMIYIKDVYKNPIIYITEN 420

BLAST of Lsi06G004990 vs. NCBI nr
Match: XP_038879813.1 (beta-glucosidase 12-like [Benincasa hispida])

HSP 1 Score: 689.9 bits (1779), Expect = 1.3e-194
Identity = 344/455 (75.60%), Postives = 369/455 (81.10%), Query Frame = 0

Query: 1   MMGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAA 60
           M+GFK DTFFLG+L LIL +VS+AA ++PSSPIPIIRRSNF KD VFGSSSAAYQYEGAA
Sbjct: 1   MLGFK-DTFFLGFLSLIL-LVSDAA-HQPSSPIPIIRRSNFPKDFVFGSSSAAYQYEGAA 60

Query: 61  FKYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSR 120
           F+YGR PSIWDTYTH H ERIADGKNGD AVDQYHRYKEDV IM+ IGFDAYRFSISWSR
Sbjct: 61  FQYGRKPSIWDTYTHKHPERIADGKNGDIAVDQYHRYKEDVAIMDRIGFDAYRFSISWSR 120

Query: 121 VLP--------------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIIN 180
           VLP                           IQSYVTIFHWDVPQALQD+YQGF+   IIN
Sbjct: 121 VLPKGKLSGGVNKEGIDYYNRLINELLSKGIQSYVTIFHWDVPQALQDEYQGFMNHSIIN 180

Query: 181 DYRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTE 240
           DYRDFA LCFKEFGDRVKHWITFNEQYVF INGYATG+FAPGRCSSWQPFNCLGGDSGTE
Sbjct: 181 DYRDFAELCFKEFGDRVKHWITFNEQYVFIINGYATGSFAPGRCSSWQPFNCLGGDSGTE 240

Query: 241 PYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN---------------------- 300
           PY VGHH IL+HA A KLYKS+YQ +QKGEIGVTLFSN                      
Sbjct: 241 PYIVGHHQILSHAVAVKLYKSKYQKYQKGEIGVTLFSNWFVPYSSSAADRNATVRALDFQ 300

Query: 301 --WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVD 360
             WFLHP+VYGDYP  MRALVK+RL  FT EE SLI  SYDFIGINYYTSNYAQ+NPNVD
Sbjct: 301 LGWFLHPVVYGDYPPIMRALVKDRLPKFTMEERSLILQSYDFIGINYYTSNYAQDNPNVD 360

Query: 361 PHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITE 406
           P +PSLLTDL AN+STDR+GVSIGPKVN+SSWL VYPEGLKDLMIHIKDHYKNP +YITE
Sbjct: 361 PARPSLLTDLRANSSTDRNGVSIGPKVNASSWLAVYPEGLKDLMIHIKDHYKNPDLYITE 420

BLAST of Lsi06G004990 vs. NCBI nr
Match: XP_038879838.1 (beta-glucosidase 12-like [Benincasa hispida])

HSP 1 Score: 685.3 bits (1767), Expect = 3.3e-193
Identity = 340/454 (74.89%), Postives = 363/454 (79.96%), Query Frame = 0

Query: 2   MGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAF 61
           M FK DTFFLG+LFLIL  V+  ATNEP +PIPI+RRSNF KD VFGSSSAAYQYEGAAF
Sbjct: 1   MAFK-DTFFLGFLFLIL--VASEATNEPKTPIPIVRRSNFPKDFVFGSSSAAYQYEGAAF 60

Query: 62  KYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRV 121
           K GRGPSIWDTYTH H ERIADGKNGD AVDQYHRYKEDV IM+ IGFDAYRFSISWSRV
Sbjct: 61  KDGRGPSIWDTYTHQHPERIADGKNGDLAVDQYHRYKEDVAIMDRIGFDAYRFSISWSRV 120

Query: 122 LP--------------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIIND 181
           LP                           IQSYVTIFHWDVPQALQD+YQGF+ ++I+ D
Sbjct: 121 LPKGKLSGGVNKKGIDYYNKLINELLSKGIQSYVTIFHWDVPQALQDEYQGFMSRKILKD 180

Query: 182 YRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEP 241
           YRDFA LCFKEFGDRVKHWITFNEQYVFTINGYA G FAPGRCSSWQP NCLGGDSGTEP
Sbjct: 181 YRDFAELCFKEFGDRVKHWITFNEQYVFTINGYAIGVFAPGRCSSWQPLNCLGGDSGTEP 240

Query: 242 YRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN----------------------- 301
           Y V HHLILAHA A +LYKS+YQA+QKGEIGVTLFSN                       
Sbjct: 241 YIVAHHLILAHALAARLYKSKYQAYQKGEIGVTLFSNWFVPYSKSEADKKATGRALDFQL 300

Query: 302 -WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDP 361
            WFL PIVYGDYPASMR LVKERL  FTKEE SLI  SYDFIGINYYTSNY QNNPNVDP
Sbjct: 301 GWFLDPIVYGDYPASMRTLVKERLPKFTKEERSLILKSYDFIGINYYTSNYVQNNPNVDP 360

Query: 362 HKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITEN 406
           +KPSLLTDL AN+STDR+GVSIGPKVN SSWL VYPEGLKDLMIH+K++YKNP+IYITEN
Sbjct: 361 NKPSLLTDLRANSSTDRNGVSIGPKVNGSSWLAVYPEGLKDLMIHMKNNYKNPIIYITEN 420

BLAST of Lsi06G004990 vs. NCBI nr
Match: XP_011660113.1 (beta-glucosidase 12 [Cucumis sativus] >KGN66763.2 hypothetical protein Csa_007267 [Cucumis sativus])

HSP 1 Score: 674.1 bits (1738), Expect = 7.6e-190
Identity = 335/455 (73.63%), Postives = 362/455 (79.56%), Query Frame = 0

Query: 1   MMGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAA 60
           MMGFK  TFFLG +FLI  IVSEAA  +PSSPIPIIR+SNF KD VFGSSS+AYQYEGA 
Sbjct: 1   MMGFK-HTFFLGLVFLISLIVSEAA-RQPSSPIPIIRKSNFPKDFVFGSSSSAYQYEGAV 60

Query: 61  FKYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSR 120
              GR PSIWDTYTH H ERIADGKNGD AVD+YHRYKEDV IM  IGF AYRFSISWSR
Sbjct: 61  DIDGRKPSIWDTYTHKHPERIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSR 120

Query: 121 VLP--------------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIIN 180
           +LP                           IQSYVTIFHWDVPQAL+D YQGFL  +IIN
Sbjct: 121 ILPKGKLIGGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIN 180

Query: 181 DYRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTE 240
           DY+DFA LCFKEFGDRVKHWITFNEQYVF INGY  GAFAPGRCSSWQPFNCLGG+SGTE
Sbjct: 181 DYQDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCLGGNSGTE 240

Query: 241 PYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN---------------------- 300
           PY VGH+ IL+HA A K+YKS+YQAHQKGEIGVTLFSN                      
Sbjct: 241 PYIVGHYQILSHAAAVKIYKSKYQAHQKGEIGVTLFSNWFVPYSNSEADRNATVRALDFQ 300

Query: 301 --WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVD 360
             WFL+P+VYGDYPASM+ALVK+RL  FTKEET LI GSYDFIGINYYTSNYAQNNPNVD
Sbjct: 301 LGWFLNPVVYGDYPASMKALVKDRLPKFTKEETKLINGSYDFIGINYYTSNYAQNNPNVD 360

Query: 361 PHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITE 406
           P KPSLLTDL AN+STDR+GVSIGPKVN+SSWL VYPEGLKDLMIHIK+HYKNP +YITE
Sbjct: 361 PSKPSLLTDLRANSSTDRNGVSIGPKVNASSWLAVYPEGLKDLMIHIKNHYKNPNLYITE 420

BLAST of Lsi06G004990 vs. NCBI nr
Match: XP_008450455.1 (PREDICTED: beta-glucosidase 12-like [Cucumis melo] >KAA0040143.1 beta-glucosidase 12-like [Cucumis melo var. makuwa] >TYK27649.1 beta-glucosidase 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 661.4 bits (1705), Expect = 5.1e-186
Identity = 328/455 (72.09%), Postives = 359/455 (78.90%), Query Frame = 0

Query: 1   MMGFKDDTFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAA 60
           MMGF+  TFFLG +FLI  IVSE A+++PSSPIPI+RRSNF K+ VFGSSS+AYQYEGA 
Sbjct: 1   MMGFR-HTFFLGLVFLISLIVSE-ASHQPSSPIPIVRRSNFPKNFVFGSSSSAYQYEGAV 60

Query: 61  FKYGRGPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSR 120
              GR PSIWDTYTH H ERIADGKNGD AVD+YHRYKEDV IM  IGF AYRFSISWSR
Sbjct: 61  ATDGRKPSIWDTYTHKHPERIADGKNGDIAVDEYHRYKEDVAIMKRIGFGAYRFSISWSR 120

Query: 121 VLPS--------------------------IQSYVTIFHWDVPQALQDKYQGFLGKEIIN 180
           ++P+                          IQSYVTIFHWDVPQAL+D YQGFL  +IIN
Sbjct: 121 IIPTGKLKDGVNKKGIDYYNRLINELLSKGIQSYVTIFHWDVPQALEDAYQGFLSPKIIN 180

Query: 181 DYRDFANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTE 240
           DY DFA LCFKEFGDRVKHWITFNEQYVF INGY  GAFAPGRCSSWQPFNC GG+SGTE
Sbjct: 181 DYGDFAELCFKEFGDRVKHWITFNEQYVFIINGYGVGAFAPGRCSSWQPFNCTGGNSGTE 240

Query: 241 PYRVGHHLILAHATAFKLYKSEYQAHQKGEIGVTLFSN---------------------- 300
           PY VGHH IL+HA A K+YKS+YQA QKGEIGVTLFSN                      
Sbjct: 241 PYIVGHHQILSHAVAVKIYKSKYQARQKGEIGVTLFSNWFVPYSNSEADRNATVRALDFQ 300

Query: 301 --WFLHPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVD 360
             WFL P+V+GDYPA+M+ALVK+RL  FTKE+  LI GSYDFIGINYYTSNYAQNNPNVD
Sbjct: 301 LGWFLDPVVHGDYPANMKALVKDRLPKFTKEQIKLINGSYDFIGINYYTSNYAQNNPNVD 360

Query: 361 PHKPSLLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITE 406
           P KPSLLTDL AN+STDR+GVSIGPKVN+SSWL VYPEGLKDLMIHIK+HYKNP +YITE
Sbjct: 361 PSKPSLLTDLRANSSTDRNGVSIGPKVNASSWLAVYPEGLKDLMIHIKNHYKNPNLYITE 420

BLAST of Lsi06G004990 vs. NCBI nr
Match: XP_038879840.1 (beta-glucosidase 12-like [Benincasa hispida])

HSP 1 Score: 641.7 bits (1654), Expect = 4.2e-180
Identity = 316/426 (74.18%), Postives = 335/426 (78.64%), Query Frame = 0

Query: 30  SSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTYTHNHSERIADGKNGDK 89
           SSPIP IRRSNFSK  VFGSSS+AYQYEGAA  YG+GPSIWD+YTH H ERI D  NGD 
Sbjct: 5   SSPIPNIRRSNFSKSFVFGSSSSAYQYEGAASMYGKGPSIWDSYTHQHPERIVDCSNGDL 64

Query: 90  AVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP-------------------------- 149
           AVDQYHRYKEDV IM +IGFDAYRFSISWSRVLP                          
Sbjct: 65  AVDQYHRYKEDVAIMKNIGFDAYRFSISWSRVLPKGKLSGGVNQEGIDYYNRLINELLSK 124

Query: 150 SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEFGDRVKHWITFNEQYVF 209
            IQ YVTIFHWDVPQAL+D+YQGFLG +IINDYRDFA+LCFKEFGDRVKHWITFNEQY+F
Sbjct: 125 GIQPYVTIFHWDVPQALEDEYQGFLGYQIINDYRDFADLCFKEFGDRVKHWITFNEQYIF 184

Query: 210 TINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHATAFKLYKSEYQAHQKG 269
            +NGYATG FAPGR SS QPFNCLGGDSGTEPY VGHH ILAHAT+ KLYKS+Y A Q G
Sbjct: 185 ALNGYATGEFAPGRGSSSQPFNCLGGDSGTEPYLVGHHQILAHATSVKLYKSKYHADQNG 244

Query: 270 EIGVTLFSN------------------------WFLHPIVYGDYPASMRALVKERLLNFT 329
           EIGVTL+S+                        WFLHPIVYGDYP SMRALVKERL  FT
Sbjct: 245 EIGVTLYSDWFVPYSSSDEDKNATSRALDFSLGWFLHPIVYGDYPTSMRALVKERLPKFT 304

Query: 330 KEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHANTSTDRDGVSIGPKVNS 389
           KEET LI GSYDFIGINYYTSNYAQNN NVDPHKP+LLTD HANTSTDR+GVSIGPKVN 
Sbjct: 305 KEETILIKGSYDFIGINYYTSNYAQNNTNVDPHKPNLLTDPHANTSTDRNGVSIGPKVNG 364

Query: 390 SSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFDIPQVYKLIKDEGKVKYYRQHLN 406
           SSWL VYPEGLKDLMIHIK+HYKNP IYITENGYLDFD P V  LIKDEG+VKYY+QHL 
Sbjct: 365 SSWLAVYPEGLKDLMIHIKNHYKNPFIYITENGYLDFDSPDVSTLIKDEGRVKYYQQHLE 424

BLAST of Lsi06G004990 vs. TAIR 10
Match: AT5G44640.1 (beta glucosidase 13 )

HSP 1 Score: 388.3 bits (996), Expect = 7.8e-108
Identity = 194/449 (43.21%), Postives = 274/449 (61.02%), Query Frame = 0

Query: 8   TFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGP 67
           T +   L  I+ + S     +  S  P +RRS+F KD +FG++++AYQ EGAA + GRGP
Sbjct: 3   TKYFSLLVFIIVLASNEVIAKKHSSTPKLRRSDFPKDFIFGAATSAYQVEGAAHEDGRGP 62

Query: 68  SIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP---- 127
           SIWDT++  + E+I DG NG  A D YH YKEDV +++ IGF AYRFSISWSR+LP    
Sbjct: 63  SIWDTFSEKYPEKIKDGTNGSIASDSYHLYKEDVGLLHQIGFGAYRFSISWSRILPRGNL 122

Query: 128 ----------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFAN 187
                                  I+ + TIFHWD PQ+L+D Y GF G EI+ND+RD+A+
Sbjct: 123 KGGINQAGIDYYNNLINELLSKGIKPFATIFHWDTPQSLEDAYGGFFGAEIVNDFRDYAD 182

Query: 188 LCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHH 247
           +CFK FGDRVKHW+T NE       GY  G  APGRCS +   NC  G+  TEPY VGH+
Sbjct: 183 ICFKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHN 242

Query: 248 LILAHATAFKLYKSEYQAHQKGEIGVTLFSNW------------------------FLHP 307
           LILAH  A K+Y+ +Y+A QKG++G+ L + W                        F+ P
Sbjct: 243 LILAHGEAVKVYREKYKASQKGQVGIALNAGWNLPYTESAEDRLAAARAMAFTFDYFMEP 302

Query: 308 IVYGDYPASMRALVKE-RLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSL 367
           +V G YP  M   VK+ RL  FT +++ ++ GSYDFIGINYY+S+YA++ P       +L
Sbjct: 303 LVTGKYPVDMVNNVKDGRLPTFTAKQSKMLKGSYDFIGINYYSSSYAKDVP-CSSENVTL 362

Query: 368 LTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDF 406
            +D  A+ + +R+GV IGPK  +S WL +YP+G++DL+++ K  +K+P++YITENG  + 
Sbjct: 363 FSDPCASVTGEREGVPIGPKA-ASDWLLIYPKGIRDLLLYAKYKFKDPVMYITENGRDEA 422

BLAST of Lsi06G004990 vs. TAIR 10
Match: AT5G42260.1 (beta glucosidase 12 )

HSP 1 Score: 387.9 bits (995), Expect = 1.0e-107
Identity = 194/449 (43.21%), Postives = 274/449 (61.02%), Query Frame = 0

Query: 8   TFFLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGP 67
           T +L  L  I+ +       +  S  P +RRS+F +D +FG++++AYQ EGAA + GRGP
Sbjct: 3   TIYLSLLVFIIVLALNEVMAKKHSSTPKLRRSDFPEDFIFGAATSAYQVEGAAHEDGRGP 62

Query: 68  SIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP---- 127
           SIWDT++  + E+I DG NG  A D YH YKEDV +++ IGFDAYRFSISWSR+LP    
Sbjct: 63  SIWDTFSEKYPEKIKDGSNGSIASDSYHLYKEDVGLLHQIGFDAYRFSISWSRILPRENL 122

Query: 128 ----------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFAN 187
                                  I+ + TIFHWD PQ+L+D Y GFLG EI+ND+RD+A+
Sbjct: 123 KGGINQAGIDYYNNLINELLSKGIKPFATIFHWDTPQSLEDAYGGFLGAEIVNDFRDYAD 182

Query: 188 LCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHH 247
           +CFK FGDRVKHW+T NE       GY  G  APGRCS +   NC  G+  TEPY VGH+
Sbjct: 183 ICFKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHN 242

Query: 248 LILAHATAFKLYKSEYQAHQKGEIGVTLFSNW------------------------FLHP 307
           LILAH  A K+Y+ +Y+A QKG++G+ L + W                        F+ P
Sbjct: 243 LILAHGEAVKVYREKYKASQKGQVGIALNAGWNLPYSESAEDRLAAARAMAFTFDYFMEP 302

Query: 308 IVYGDYPASMRALVK-ERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSL 367
           +V G YP  M   VK  RL  FT +++ ++ GSYDFIG NYY+S+YA++ P       +L
Sbjct: 303 LVTGKYPIDMVNYVKGGRLPTFTAKQSKMLKGSYDFIGRNYYSSSYAKDVP-CSSENVTL 362

Query: 368 LTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDF 406
            +D  A+ + +R+GV IGPK  +S WL +YP+G++DL+++ K  +K+P++YITENG  + 
Sbjct: 363 FSDPCASVTGEREGVPIGPKA-ASDWLLIYPKGIRDLLLYAKYKFKDPVMYITENGRDEA 422

BLAST of Lsi06G004990 vs. TAIR 10
Match: AT2G44450.1 (beta glucosidase 15 )

HSP 1 Score: 387.1 bits (993), Expect = 1.7e-107
Identity = 188/446 (42.15%), Postives = 275/446 (61.66%), Query Frame = 0

Query: 10  FLGYLFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSI 69
           +L  L +++ + S       +S  P +RRS+F +D +FGS+++AYQ EG A + GRGPSI
Sbjct: 5   YLSLLVVLIVLASNDVLANNNSSTPKLRRSDFPEDFIFGSATSAYQVEGGAHEDGRGPSI 64

Query: 70  WDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP------ 129
           WDT++  + E+I DG NG  A + YH YKEDV +++ IGF+AYRFSISWSR+LP      
Sbjct: 65  WDTFSEKYPEKIKDGSNGSVADNSYHLYKEDVALLHQIGFNAYRFSISWSRILPRGNLKG 124

Query: 130 --------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLC 189
                                I+ + T+FHWD PQAL+D Y GF G EI+ND+RD+A++C
Sbjct: 125 GINQAGIDYYNNLINELLSKGIKPFATMFHWDTPQALEDAYGGFRGAEIVNDFRDYADIC 184

Query: 190 FKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLI 249
           FK FGDRVKHW+T NE       GY  G  APGRCS +   NC  G+  TEPY VGH+LI
Sbjct: 185 FKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTDGNGATEPYIVGHNLI 244

Query: 250 LAHATAFKLYKSEYQAHQKGEIGVTLFSNW------------------------FLHPIV 309
           L+H  A ++Y+ +Y+A Q+G++G+ L + W                        F+ P+V
Sbjct: 245 LSHGAAVQVYREKYKASQQGQVGIALNAGWNLPYTESPKDRLAAARAMAFTFDYFMEPLV 304

Query: 310 YGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTD 369
            G YP  M   VK RL  FT +++ ++ GSYDFIGINYY+S YA++ P       ++ +D
Sbjct: 305 TGKYPVDMVNNVKGRLPIFTAQQSKMLKGSYDFIGINYYSSTYAKDVP-CSTKDVTMFSD 364

Query: 370 LHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFDIP 406
             A+ + +RDGV IGPK  +S WL +YP+G++DL+++ K  +K+P++YITENG  +F   
Sbjct: 365 PCASVTGERDGVPIGPKA-ASDWLLIYPKGIRDLVLYAKYKFKDPVMYITENGRDEFSTN 424

BLAST of Lsi06G004990 vs. TAIR 10
Match: AT3G60130.1 (beta glucosidase 16 )

HSP 1 Score: 385.2 bits (988), Expect = 6.6e-107
Identity = 194/443 (43.79%), Postives = 275/443 (62.08%), Query Frame = 0

Query: 14  LFLILFIVSEAATNEPSSPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGRGPSIWDTY 73
           L L++ +     + +  S  P +RR++F +D VFGS+++AYQ EGAA + GRGPSIWD++
Sbjct: 8   LLLLITLACIGVSAKKHSTRPRLRRNDFPQDFVFGSATSAYQCEGAAHEDGRGPSIWDSF 67

Query: 74  THNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP---------- 133
           +    E+I DG NG  A D Y+ YKEDV +++ IGFDAYRFSISWSR+LP          
Sbjct: 68  SEKFPEKIMDGSNGSIADDSYNLYKEDVNLLHQIGFDAYRFSISWSRILPRGTLKGGINQ 127

Query: 134 ----------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDFANLCFKEF 193
                            ++ +VT+FHWD+P AL++ Y G LG E +ND+RD+A LCF++F
Sbjct: 128 AGIEYYNNLINQLISKGVKPFVTLFHWDLPDALENAYGGLLGDEFVNDFRDYAELCFQKF 187

Query: 194 GDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVGHHLILAHA 253
           GDRVK W T NE Y     GY TG  APGRCS++   +CLGGD+ TEPY VGH+L+LAH 
Sbjct: 188 GDRVKQWTTLNEPYTMVHEGYITGQKAPGRCSNFYKPDCLGGDAATEPYIVGHNLLLAHG 247

Query: 254 TAFKLYKSEYQAHQKGEIGVTLFSNW------------------------FLHPIVYGDY 313
            A K+Y+ +YQA QKGEIG+ L + W                        F+ PIVYG Y
Sbjct: 248 VAVKVYREKYQATQKGEIGIALNTAWHYPYSDSYADRLAATRATAFTFDYFMEPIVYGRY 307

Query: 314 PASMRALVKE-RLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPSLLTDLHA 373
           P  M + VK+ RL  FT EE+ ++ GSYDFIG+NYY+S YA++ P       ++ TD   
Sbjct: 308 PIEMVSHVKDGRLPTFTPEESEMLKGSYDFIGVNYYSSLYAKDVP-CATENITMTTDSCV 367

Query: 374 NTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLDFDIPQVY 406
           +   +R+GV IGP    S WL +YP+G++DL++H K  Y +P++YITENG  + +I +++
Sbjct: 368 SLVGERNGVPIGPAA-GSDWLLIYPKGIRDLLLHAKFRYNDPVLYITENGVDEANIGKIF 427

BLAST of Lsi06G004990 vs. TAIR 10
Match: AT2G44480.1 (beta glucosidase 17 )

HSP 1 Score: 359.4 bits (921), Expect = 3.9e-99
Identity = 188/452 (41.59%), Postives = 268/452 (59.29%), Query Frame = 0

Query: 8   TFFLGYLFLILFIVSEAATNEPS--SPIPIIRRSNFSKDLVFGSSSAAYQYEGAAFKYGR 67
           + F+  +  I+  +SE    +PS       ++RS+F +D  FG++S+AYQ EGAA   GR
Sbjct: 5   SIFIIIIISIITSISELYALDPSFLRLSTSLQRSSFPQDFRFGAASSAYQSEGAANVDGR 64

Query: 68  GPSIWDTYTHNHSERIADGKNGDKAVDQYHRYKEDVVIMNHIGFDAYRFSISWSRVLP-- 127
            PSIWDT+T  + E+I+DG NGD A + Y+R+KEDV  M  IG D++RFSISWSR+LP  
Sbjct: 65  EPSIWDTFTKQYPEKISDGSNGDVADEFYYRFKEDVAHMKEIGLDSFRFSISWSRILPRG 124

Query: 128 ------------------------SIQSYVTIFHWDVPQALQDKYQGFLGKEIINDYRDF 187
                                    I+  VT+FHWD PQAL+D+Y GFL  +I+ D+ ++
Sbjct: 125 TVAGGVNQAGINFYNHLINELISNGIRPLVTLFHWDTPQALEDEYGGFLNPQIVKDFVEY 184

Query: 188 ANLCFKEFGDRVKHWITFNEQYVFTINGYATGAFAPGRCSSWQPFNCLGGDSGTEPYRVG 247
            ++CFKEFGDRVK WIT NE  +F + GY  G  APGRCSS+   NC  G+S TEPY V 
Sbjct: 185 VDICFKEFGDRVKEWITINEPNMFAVLGYNVGNIAPGRCSSYVQ-NCTVGNSATEPYLVA 244

Query: 248 HHLILAHATAFKLYKSEYQAHQKGEIGVTL------------------------FSNWFL 307
           H+LIL+HA   +LY+ +YQ+   G IG+T+                        F  WF 
Sbjct: 245 HYLILSHAATVQLYREKYQSFHGGTIGMTIQTYWMIPKYNTPACREAAKRALDFFFGWFA 304

Query: 308 HPIVYGDYPASMRALVKERLLNFTKEETSLITGSYDFIGINYYTSNYAQNNPNVDPHKPS 367
            PI YGDYP +MR LV  RL  FTK+++ ++ GS+DF G+NYYTS Y ++         S
Sbjct: 305 DPITYGDYPKTMRELVGNRLPKFTKKQSKMVRGSFDFFGLNYYTSRYVEDVMFYANTNLS 364

Query: 368 LLTDLHANTSTDRDGVSIGPKVNSSSWLPVYPEGLKDLMIHIKDHYKNPLIYITENGYLD 406
             TD   N +T+++GV +G +  S+ WL + PEG +D++++IK  ++NP+I +TENG   
Sbjct: 365 YTTDSRVNQTTEKNGVPVG-EPTSADWLFICPEGFQDVLLYIKSKFQNPVILVTENGMPS 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B8AVF01.7e-12349.10Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1[more]
Q7XKV42.9e-12349.10Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2[more]
Q7XKV21.8e-11747.60Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2[more]
Q7XKV51.3e-11546.35Beta-glucosidase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU11 PE=3 SV=2[more]
P262053.7e-11549.50Cyanogenic beta-glucosidase (Fragment) OS=Trifolium repens OX=3899 GN=LI PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A5D3DW452.5e-18672.09Beta-glucosidase 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BNM62.5e-18672.09beta-glucosidase 12-like OS=Cucumis melo OX=3656 GN=LOC103492050 PE=3 SV=1[more]
A0A0A0LXF33.4e-18071.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G611300 PE=3 SV=1[more]
A0A6J1JG746.8e-16063.18beta-glucosidase 12-like OS=Cucurbita maxima OX=3661 GN=LOC111485433 PE=3 SV=1[more]
A0A6J1H8A01.5e-15961.23beta-glucosidase 12-like OS=Cucurbita moschata OX=3662 GN=LOC111461397 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038879813.11.3e-19475.60beta-glucosidase 12-like [Benincasa hispida][more]
XP_038879838.13.3e-19374.89beta-glucosidase 12-like [Benincasa hispida][more]
XP_011660113.17.6e-19073.63beta-glucosidase 12 [Cucumis sativus] >KGN66763.2 hypothetical protein Csa_00726... [more]
XP_008450455.15.1e-18672.09PREDICTED: beta-glucosidase 12-like [Cucumis melo] >KAA0040143.1 beta-glucosidas... [more]
XP_038879840.14.2e-18074.18beta-glucosidase 12-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G44640.17.8e-10843.21beta glucosidase 13 [more]
AT5G42260.11.0e-10743.21beta glucosidase 12 [more]
AT2G44450.11.7e-10742.15beta glucosidase 15 [more]
AT3G60130.16.6e-10743.79beta glucosidase 16 [more]
AT2G44480.13.9e-9941.59beta glucosidase 17 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001360Glycoside hydrolase family 1PRINTSPR00131GLHYDRLASE1coord: 366..374
score: 60.59
coord: 387..398
score: 33.11
coord: 289..303
score: 51.73
IPR001360Glycoside hydrolase family 1PFAMPF00232Glyco_hydro_1coord: 125..252
e-value: 6.7E-37
score: 127.0
coord: 39..124
e-value: 9.2E-32
score: 110.0
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 28..124
coord: 125..254
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 252..405
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 21..251
e-value: 4.7E-92
score: 311.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 252..406
e-value: 5.8E-46
score: 158.8
NoneNo IPR availablePANTHERPTHR10353:SF272SUBFAMILY NOT NAMEDcoord: 125..254
NoneNo IPR availablePANTHERPTHR10353:SF272SUBFAMILY NOT NAMEDcoord: 28..124
coord: 252..405
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 35..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G004990.1Lsi06G004990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds