Lsi06G004940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G004940
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Beta-glucosidase
Location	chr06: 5820818 .. 5824557 (+)
RNA-Seq Expression	Lsi06G004940
Synteny	Lsi06G004940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTGTCAATTTACTCCTAGAATATTAAATCTTACGTAGGTGGCTACTATGGATTGAACCCACAACCTTACCACTAGGCCAACCCATGATGGTTAAAAAAATGTAAAATGAGCTTTACAACTAAAAGATGAGATATTTTAATTCAAATATAGTAAAATGAGCTAATTTATTTATAAATATAGCAAAAATTTGTTGCCTATCAACGATAGATAGTGATTGACAATAATAGATAATAGTAGTCTATCACTGTCTATCGTTGATAGATAGTGAGATTTTACTATTTTGAAAATATTTTCATCTGTTTTGCCATTTAAAATAATTACTCAAAAAAAATTAAATTTTTTTTAATATTAACAATTTTTCTAGAATTTCAAAAGTTCTTTAAGAAGGAAATATAAAAGCTAATTCTATTAAATTAATTGCAGGGATGATTTTGTGGACTATGTCGACCTTTGCTTTCGACACTTTGGTGATAGAGTTAAGCATTGGATCACTTTAAACGAACCAAATTTGTTTGCTAGAAACGGTTATGCGACAGGAGTATTTGCACCAGGAAGATGTTCAAGATGGGTACTCAATAATTGTTGTGATGGGAATTCTTCAATTGAACCATATATAGTCAGTCACAACGAAATTCTTGCTCATGGTCGTGCTGTCAAACTTTACCGAACGAAATATCAGGTTTTCAATTTGTCAAACTACAACAATACACAATAGTTACATTCTAGGATAATGAACATCTGCCATTATTTAATATGCTATTTTAAAATTATGTCATATTCAAAATCACTCTCAAACATGCTTTTAATCATTAATCATTAATGCAATAAAAATCACTAGAGAATGTTTCACGATTCAATTCGGTTCAGTCTACCTTCAAAAAAACATATATATACCCGTACTGAAATGCACATATTTGGTTTGGGCTTTTTTATTCTATATTTAATATAAATCTTTATTATTTTAAAGAAAAACTTTCACAGATAGAAAAAATGTCAAACTGTTTATAGAAAATAGCAAAAAAAACACTGATAGACATTGATAGACTTCTATCATTTCTATCACTAATAGACCTTGATAGAATACTATCCGCGTCTATCACAACTATCTAAAAGAATTGCTATTTTGTGTAAATAATTTCCTTTATATATGTTTCTATTTTTAAAATTCCTCTATTTAAATTGAACTAAACCGACAATTCAGTAAAAACTAAATTTTCAGTTCTGTTCGGTTTAGTTTTCTCAGTTGTTTCGGTTTGGTTAAATTTAGTGTTCACCCCTAAAAAATCATGTTTAAAAGTATAAAATCAAAACAAAGTGAAAGTAATTTTAACCAAAGTGATTTTAATCGTTTCAAAATACTCTCGAACATGATTTTACAACATGTTTTAGGAGATTTTGCTTCCCACCATTATGTTAATCACAATTAACTCTTTCATATATTTTTTAAAAACATTACACATTGAATATTTACAGGAAAAACAAAAGGGTGTGATTGGCATAACAGTGTTAGCAAATTGGTACGAACCTCATTCAGAAACTGCCAAAGACAAAGAAGCTGCAAAACGAGCTACTGAGTTCTTTTCTGGTTGGTAAGTAAATTAAAGTCCGTTTCACAATCGTAAAAAGTAAAAATATTTTAACGGTCTAGTCTGTTTGGTAAAACAAATTATTTATTAATTTATCCATAATCACTATCTCAAAAGTTTATATAAATCAATAATTTTACTAGAATATACATTAAAACTTCCATAGATTTCAATTATATCATTATATAACTCAATTTTTAACCAAATTAAATGCATGGATATTAGGTTCTATAATCCAATTGTCCATGGTGATTATCCAAGAAGTATGAGAATTCGTGTAAAAGATAGACTGCCAAAATTCACAGAAGAAGAAAAAGATTTGATAAAAGGAAGCTTTGATTTTCTTGGACTTAACTACTATACAGCTGCATATGCCATAGATGATTCCAATACACATGTTCTATATCCTTCATGCCTTACAGATTCTGGTGTAAATACTTCAAGTATGTACTTCTAAACTATATTATATTCAAAATAACATATGCTTTTTCAATGATATTTGATTACATTTCATAATCTAATATTCTAACTTTTGAAAAATAATCAAACCATACCGTTGATTTTATTAAAGTTTCGTTTGATAATCATTTAGTTTTTAAAAATAAAGTTTACTTTATTGTTTAGTGGAATTTTTAAAAAATAGAAAAATAAGGAGAACTATTTAAACAAAATAGCAAAATTTTTAGATAGTTGTGATCGATGCTGATTTTTTAAAAATAATTTGACATTTTTTCTATTTGTGAAAATTTCCATATTTTTTATTCATCCTTCTTAAGTAATTGAATTCTTACCCAAATTCCACGAACAAAAACTAGTTTTTATAAACTACTATTTTAGTGTTCAAAACTAATTGGCTTAGAGTTTTTGAAAATATTGATAGAAAGTATATAACGAAGCAAGAAATGTATAGGTGGAAGGAGTGTTTATAGGATTAAATTTTAAAAACTAAAAACCAAAAACCAAATGATTACCGAATGGGACCTTGATTTTTTATTTTTGAAATTTAATCTTATAAACACTACTTTTACCTATTGATAACTTCATTTTGTTATCAACTTTTCAGGAGTGTTTTCAAAAACTAAGTCAAGTTTTGAGAAACTTATTTATTTATTAATTTGAAGTTTGAGTTAAGAATTCAAATATTTAGTTAAAAAAAAAAAGAAGATGAAACTAAGGACAAATATTGTAGGAAAACAACATAATTTTCTAAAATAAAAAATAAAAAATAAAATACTTATTACAATGAAGGGAACTTTTCACAAATAGAAAAATGTCAAACTATTTACAAAAATAGCAAAAAAAAAAAAAAAAAACCACTGATAGACATTGATAGACTTCTATCAACATTTATCAGTGATAGATTTCTTTCATTGCTATCACTTATAGACGCTGATAGACTTCTATGAGCGTCTGTCCCAATTATCTAAAAAATTTGATATTTTGTGTAAATAATTTCCCTTATTTTCTATTTTTGAAAATTTCCCTTAAATGAAGCCTAAATTCTTTAGAGTTTTGTTTTTCTTTAATTTTTAATTTATTAAAATGTGACTTAAAAATATTACTCTTCAAAACCCTTCAAGTCTACTTATATTAAGGTTGGTTTTCTTGTGCAAGTCATGAAATAATATTTGAAAATCACTAATTAAATTTTGTATAATGTGCTTGGGATTTGCTGCTGATTGTTTCATGGAGTTTCACTTGGACCAAAGGTAATTAAGTATGTAAATTTTCCTAAATATTGAAAACATGAATTTCTCATGCTATAATTTTCAATTAGGTTTTTACATTTTTCTTATTTGATCGAACAATTTGCTATATCGTTTCATGTGGTAGGTGAATGCATCATCTTGGCTTTCAGTTTATCCACTCGGATTGAAAAAATTGTTGGTAAACATGAAAAATGATTATAACAATCCAGATATCTACATCACGGAAAATGGTATTGAAAAAAATATCGTTTTTCTTTTCGTATATCAAGAAAAATAAATAACACCATATTTAATTATCTTATTTATTAATCATAAATTGTACTTATGCAGGATACTTTGACTATGATGATCCTGATGTAGATAAACTAATTTGTGATAAGAAGAGAACTCTTTACCATATAGCACATCTCTATTATCTCCTCCAAGCAATAGTCACGTAA

mRNA sequence

ATGGGGGATGATTTTGTGGACTATGTCGACCTTTGCTTTCGACACTTTGGTGATAGAGTTAAGCATTGGATCACTTTAAACGAACCAAATTTGTTTGCTAGAAACGGTTATGCGACAGGAGTATTTGCACCAGGAAGATGTTCAAGATGGGTACTCAATAATTGTTGTGATGGGAATTCTTCAATTGAACCATATATAGTCAGTCACAACGAAATTCTTGCTCATGGTCGTGCTGTCAAACTTTACCGAACGAAATATCAGGAAAAACAAAAGGGTGTGATTGGCATAACAGTGTTAGCAAATTGGTACGAACCTCATTCAGAAACTGCCAAAGACAAAGAAGCTGCAAAACGAGCTACTGAGTTCTATAATCCAATTGTCCATGGTGATTATCCAAGAAGTATGAGAATTCGTGTAAAAGATAGACTGCCAAAATTCACAGAAGAAGAAAAAGATTTGATAAAAGGAAGCTTTGATTTTCTTGGACTTAACTACTATACAGCTGCATATGCCATAGATGATTCCAATACACATGTTCTATATCCTTCATGCCTTACAGATTCTGGTGTGAATGCATCATCTTGGCTTTCAGTTTATCCACTCGGATTGAAAAAATTGTTGGTAAACATGAAAAATGATTATAACAATCCAGATATCTACATCACGGAAAATGGATACTTTGACTATGATGATCCTGATGTAGATAAACTAATTTGTGATAAGAAGAGAACTCTTTACCATATAGCACATCTCTATTATCTCCTCCAAGCAATAGTCACGTAA

Coding sequence (CDS)

ATGGGGGATGATTTTGTGGACTATGTCGACCTTTGCTTTCGACACTTTGGTGATAGAGTTAAGCATTGGATCACTTTAAACGAACCAAATTTGTTTGCTAGAAACGGTTATGCGACAGGAGTATTTGCACCAGGAAGATGTTCAAGATGGGTACTCAATAATTGTTGTGATGGGAATTCTTCAATTGAACCATATATAGTCAGTCACAACGAAATTCTTGCTCATGGTCGTGCTGTCAAACTTTACCGAACGAAATATCAGGAAAAACAAAAGGGTGTGATTGGCATAACAGTGTTAGCAAATTGGTACGAACCTCATTCAGAAACTGCCAAAGACAAAGAAGCTGCAAAACGAGCTACTGAGTTCTATAATCCAATTGTCCATGGTGATTATCCAAGAAGTATGAGAATTCGTGTAAAAGATAGACTGCCAAAATTCACAGAAGAAGAAAAAGATTTGATAAAAGGAAGCTTTGATTTTCTTGGACTTAACTACTATACAGCTGCATATGCCATAGATGATTCCAATACACATGTTCTATATCCTTCATGCCTTACAGATTCTGGTGTGAATGCATCATCTTGGCTTTCAGTTTATCCACTCGGATTGAAAAAATTGTTGGTAAACATGAAAAATGATTATAACAATCCAGATATCTACATCACGGAAAATGGATACTTTGACTATGATGATCCTGATGTAGATAAACTAATTTGTGATAAGAAGAGAACTCTTTACCATATAGCACATCTCTATTATCTCCTCCAAGCAATAGTCACGTAA

Protein sequence

MGDDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSIEPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATEFYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNTHVLYPSCLTDSGVNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYFDYDDPDVDKLICDKKRTLYHIAHLYYLLQAIVT

Homology

[Close]

BLAST of Lsi06G004940 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-68
Identity = 131/277 (47.29%), Postives = 178/277 (64.26%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DY ++CF+ FGDRVK+WIT NEP  F  NGYATG+FAPGRCS W   NC  G+S  
Sbjct: 178 NDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGR 237

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPY   H+++LAH   V+LY+ KYQ  QKG IGIT++++W+ P S +  + +AAKRA + 
Sbjct: 238 EPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNNDAAKRAIDF 297

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F +P++ GDYP SMR  V +RLP+FT+E+  L+KG+FDF+GLNYYTA YA +   +
Sbjct: 298 MFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPS 357

Query: 183 HVLYPSCLTDSGVN--------------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYITE 242
           + L  S  TDS  N              AS WL VYP G + LL+ +K +Y NP +YITE
Sbjct: 358 NGLNNSYTTDSRANLTGVRNGIPIGPQAASPWLYVYPQGFRDLLLYVKENYGNPTVYITE 417

Query: 243 NGYFDYDDP--DVDKLICDKKRTLYHIAHLYYLLQAI 259
           NG  ++++    + + + D  R  Y+  HL  LL AI
Sbjct: 418 NGVDEFNNKTLPLQEALKDDARIEYYHKHLLSLLSAI 454

[Close]

BLAST of Lsi06G004940 vs. ExPASy Swiss-Prot
Match: Q7XKV4 (Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2)

HSP 1 Score: 260.0 bits (663), Expect = 2.9e-68
Identity = 131/277 (47.29%), Postives = 178/277 (64.26%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DY ++CF+ FGDRVK+WIT NEP  F  NGYATG+FAPGRCS W   NC  G+S  
Sbjct: 178 NDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGR 237

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPY   H+++LAH   V+LY+ KYQ  QKG IGIT++++W+ P S +  + +AAKRA + 
Sbjct: 238 EPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDF 297

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F +P++ GDYP SMR  V +RLP+FT+E+  L+KG+FDF+GLNYYTA YA +   +
Sbjct: 298 MFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPS 357

Query: 183 HVLYPSCLTDSGVN--------------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYITE 242
           + L  S  TDS  N              AS WL VYP G + LL+ +K +Y NP +YITE
Sbjct: 358 NGLNNSYTTDSRANLTGVRNGIPIGPQAASPWLYVYPQGFRDLLLYVKENYGNPTVYITE 417

Query: 243 NGYFDYDDP--DVDKLICDKKRTLYHIAHLYYLLQAI 259
           NG  ++++    + + + D  R  Y+  HL  LL AI
Sbjct: 418 NGVDEFNNKTLPLQEALKDDARIEYYHKHLLSLLSAI 454

[Close]

BLAST of Lsi06G004940 vs. ExPASy Swiss-Prot
Match: P26205 (Cyanogenic beta-glucosidase (Fragment) OS=Trifolium repens OX=3899 GN=LI PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.9e-67
Identity = 125/242 (51.65%), Postives = 159/242 (65.70%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           DDF DY +LCF+ FGDRVKHWITLNEP   + N YA G FAPGRCS W+  NC  G+S  
Sbjct: 169 DDFRDYAELCFKEFGDRVKHWITLNEPWGVSMNAYAYGTFAPGRCSDWLKLNCTGGDSGR 228

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPY+ +H ++LAH  A +LY+TKYQ  Q G+IGIT++++W+EP S+   D +AAKR  + 
Sbjct: 229 EPYLAAHYQLLAHAAAARLYKTKYQASQNGIIGITLVSHWFEPASKEKADVDAAKRGLDF 288

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F +P+  G YP SMR  V+ RLPKF+ EE   + GSFDFLGLNYY++ YA      
Sbjct: 289 MLGWFMHPLTKGRYPESMRYLVRKRLPKFSTEESKELTGSFDFLGLNYYSSYYAAKAPRI 348

Query: 183 HVLYPSCLTDSGVN--------------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYITE 226
               P+  TDS +N              ASSWL +YP G++KLL+ +KN YNNP IYITE
Sbjct: 349 PNARPAIQTDSLINATFEHNGKPLGPMAASSWLCIYPQGIRKLLLYVKNHYNNPVIYITE 408

[Close]

BLAST of Lsi06G004940 vs. ExPASy Swiss-Prot
Match: Q5Z9Z0 (Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.1e-66
Identity = 129/277 (46.57%), Postives = 178/277 (64.26%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DY D+CFR FGDRVK+WIT NEP  F+  GY+ G+ APGRCS    + C  G+S  
Sbjct: 172 EDFRDYADICFREFGDRVKYWITFNEPWSFSIGGYSNGILAPGRCSSQGKSGCSKGDSGR 231

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV+HN++LAH   V++YR KYQ  QKG IGI +++NW  P+ ++ +DK A KRA + 
Sbjct: 232 EPYIVAHNQLLAHAAVVQIYREKYQGGQKGKIGIAIVSNWMIPYEDSKEDKHATKRALDF 291

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAY---AIDD 182
               F +P+  GDYP SMR  V +RLP+FT+E+   I GSFDF+GLNYYTA Y      D
Sbjct: 292 MYGWFMDPLTKGDYPVSMRTLVGNRLPRFTKEQSKAINGSFDFIGLNYYTARYIQGTKQD 351

Query: 183 SNTHVLYPSCL----------TDSGVNA-SSWLSVYPLGLKKLLVNMKNDYNNPDIYITE 242
           SN+H  Y +            TD G  A SSWL +YP G+++LL+  K  YNNP IYITE
Sbjct: 352 SNSHKSYSTDSLTNERVERNGTDIGPKAGSSWLYIYPKGIEELLLYTKRTYNNPTIYITE 411

Query: 243 NGYFDYDDPDVD--KLICDKKRTLYHIAHLYYLLQAI 259
           NG  + ++ ++   + + D  R  ++  HL+++ +A+
Sbjct: 412 NGVDEVNNENLSLKEALIDTTRIEFYRQHLFHVQRAL 448

[Close]

BLAST of Lsi06G004940 vs. ExPASy Swiss-Prot
Match: A3RF67 (Isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase OS=Dalbergia nigrescens OX=298683 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.7e-66
Identity = 128/277 (46.21%), Postives = 173/277 (62.45%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DY DLCF  FGDRVKHWIT+NEP++F  NGYA G+FAPGRCS      C  G++  
Sbjct: 180 NDFRDYADLCFNLFGDRVKHWITVNEPSIFTMNGYAYGIFAPGRCSPSYNPTCTGGDAGT 239

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EP +V+HN IL+H   V++Y+ KYQE Q G+IGI++   W  P S +  D++AA+R  + 
Sbjct: 240 EPDLVAHNLILSHAATVQVYKKKYQEHQNGIIGISLQIIWAVPLSNSTSDQKAAQRYLDF 299

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F +P+  G YP SM+  V DRLPKFT +E  L+KGSFDF+G+NYYT++Y      +
Sbjct: 300 TGGWFLDPLTAGQYPESMQYLVGDRLPKFTTDEAKLVKGSFDFVGINYYTSSYLTSSDAS 359

Query: 183 HVLYPSCLTDSGVNASS--------------WLSVYPLGLKKLLVNMKNDYNNPDIYITE 242
               PS LTDS V  SS              W+ +YP GL+ LL+ +K  YNNP +YITE
Sbjct: 360 TCCPPSYLTDSQVTFSSQRNGVFIGPVTPSGWMCIYPKGLRDLLLYIKEKYNNPLVYITE 419

Query: 243 NGYFDYDDP--DVDKLICDKKRTLYHIAHLYYLLQAI 259
           NG  + DDP   +++ + D  R   +  HL+Y+  AI
Sbjct: 420 NGMDELDDPSQSLEESLIDTYRIDSYYRHLFYVRSAI 456

[Close]

BLAST of Lsi06G004940 vs. ExPASy TrEMBL
Match: A0A6J1JKM7 (cyanogenic beta-glucosidase-like OS=Cucurbita maxima OX=3661 GN=LOC111485436 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.5e-115
Identity = 198/272 (72.79%), Postives = 226/272 (83.09%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           DDF  YVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNCCDG+SS 
Sbjct: 174 DDFEGYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWMLNNCCDGDSSK 233

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAV +YRTKYQ  QKGVIGITV+ NWYEP+S+T  D+EAAKRA E 
Sbjct: 234 EPYIVGHNQILAHARAVDVYRTKYQRTQKGVIGITVIGNWYEPYSDTCADEEAAKRAIEF 293

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVK+RLP FT++E  LIKGS+DFLGLNYYTAAYAIDDS+ 
Sbjct: 294 FSGWFFNPIVHGDYPQSMRLRVKERLPTFTQDEATLIKGSYDFLGLNYYTAAYAIDDSSA 353

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS LTDSG          VNASSWLS+YP GLKKLL+ MKNDYNNP IYITENG+F
Sbjct: 354 HVLYPSWLTDSGVNTSISLGPKVNASSWLSIYPEGLKKLLITMKNDYNNPSIYITENGFF 413

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAIV 260
           DYDDP   KL+CD+KRT YH+ HLY+L +A++
Sbjct: 414 DYDDPSARKLLCDRKRTRYHMGHLYFLHEAMM 445

[Close]

BLAST of Lsi06G004940 vs. ExPASy TrEMBL
Match: A0A6J1HBR5 (beta-glucosidase 12-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461398 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.8e-113
Identity = 196/271 (72.32%), Postives = 222/271 (81.92%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DYVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNC DG+SS 
Sbjct: 174 NDFEDYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWILNNCYDGDSSK 233

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAVK+YRTKYQ  QKGVIGITV+ NWYEP+S T  D+EAAKRA E 
Sbjct: 234 EPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVIGNWYEPYSNTFADEEAAKRAIEF 293

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVKDRLP FT++E  LIK S+DFLGLNYYTAAYAIDDS+ 
Sbjct: 294 FSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLIKCSYDFLGLNYYTAAYAIDDSSA 353

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS  TDSG          VNASSWLSVYP GL+KLL+ MKNDY+NP IYITENG+F
Sbjct: 354 HVLYPSWRTDSGVNTSISLGPKVNASSWLSVYPKGLQKLLITMKNDYDNPSIYITENGFF 413

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           DYDDP   KL+CD+KR  YH+ HLY+L QA+
Sbjct: 414 DYDDPSARKLLCDRKRIFYHMGHLYFLRQAM 444

[Close]

BLAST of Lsi06G004940 vs. ExPASy TrEMBL
Match: A0A6J1H9S5 (beta-glucosidase 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461398 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.8e-113
Identity = 196/271 (72.32%), Postives = 222/271 (81.92%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DYVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNC DG+SS 
Sbjct: 77  NDFEDYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWILNNCYDGDSSK 136

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAVK+YRTKYQ  QKGVIGITV+ NWYEP+S T  D+EAAKRA E 
Sbjct: 137 EPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVIGNWYEPYSNTFADEEAAKRAIEF 196

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVKDRLP FT++E  LIK S+DFLGLNYYTAAYAIDDS+ 
Sbjct: 197 FSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLIKCSYDFLGLNYYTAAYAIDDSSA 256

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS  TDSG          VNASSWLSVYP GL+KLL+ MKNDY+NP IYITENG+F
Sbjct: 257 HVLYPSWRTDSGVNTSISLGPKVNASSWLSVYPKGLQKLLITMKNDYDNPSIYITENGFF 316

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           DYDDP   KL+CD+KR  YH+ HLY+L QA+
Sbjct: 317 DYDDPSARKLLCDRKRIFYHMGHLYFLRQAM 347

[Close]

BLAST of Lsi06G004940 vs. ExPASy TrEMBL
Match: A0A6J1JG58 (beta-glucosidase 24-like OS=Cucurbita maxima OX=3661 GN=LOC111485420 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.7e-87
Identity = 161/272 (59.19%), Postives = 199/272 (73.16%), Query Frame = 0


Query: 4   DFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSIE 63
           DF D+VD+CFR FGDRVKHWIT+NEPN +   GYA G + PGRCS W ++NC  GN++ E
Sbjct: 216 DFTDFVDVCFREFGDRVKHWITINEPNTYTMMGYAVGQYPPGRCSEWQIHNCLGGNAATE 275

Query: 64  PYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE-- 123
           PYIV HN ILAH  AVK+YR KYQ+ QKGVIGITVLA+WY P ++T  D EAA RA +  
Sbjct: 276 PYIVGHNLILAHAHAVKVYRDKYQKTQKGVIGITVLADWYVPLTDTPADIEAANRAVDFF 335

Query: 124 ---FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNTH 183
              F+NP+VHG+YP +M  RVK+RLP+FTEE++ LIKGS+DFLG+NYYT+ YA +D    
Sbjct: 336 SGWFFNPVVHGNYPETMISRVKERLPQFTEEQRILIKGSYDFLGVNYYTSNYAKNDDKP- 395

Query: 184 VLYPSCLTDSG------------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGY 243
              PS LTDSG            VNASSWL+VYP GLK  L+++K  YN+P IYITENGY
Sbjct: 396 AQRPSYLTDSGAISLNEPSLGPKVNASSWLAVYPRGLKDALMDIKRKYNDPVIYITENGY 455

Query: 244 FDYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           FDYD P+  +LI DKKR  ++  HLYYL +A+
Sbjct: 456 FDYDGPNFKELIDDKKRAEFYNEHLYYLHKAM 486

[Close]

BLAST of Lsi06G004940 vs. ExPASy TrEMBL
Match: A0A6J1H894 (beta-glucosidase 24-like OS=Cucurbita moschata OX=3662 GN=LOC111461391 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 4.9e-87
Identity = 162/272 (59.56%), Postives = 199/272 (73.16%), Query Frame = 0


Query: 4   DFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSIE 63
           DF D+VD+CFR FGDRVKHWIT+NEPN +   GYA G + PGRCS W ++NC  GN++ E
Sbjct: 217 DFTDFVDVCFREFGDRVKHWITVNEPNTYTMMGYALGQYPPGRCSEWQIHNCLGGNAATE 276

Query: 64  PYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE-- 123
           PYIV HN ILAH  AVK+YR KYQ+ QKGVIGITVLA+WY P ++T  D EAA RA +  
Sbjct: 277 PYIVGHNLILAHAHAVKVYRDKYQKTQKGVIGITVLADWYVPLTDTPADIEAANRAVDFF 336

Query: 124 ---FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNTH 183
              F+NP+VHG+YP +M  RVK+RLP FTEE++ LIKGS+DFLG+NYYT+ YA +D    
Sbjct: 337 SGWFFNPVVHGNYPETMISRVKERLPHFTEEQRILIKGSYDFLGVNYYTSNYAKNDDKP- 396

Query: 184 VLYPSCLTDSG------------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGY 243
              PS LTDSG            VNASSWL+VYP GLK  L+++K  YN+P IYITENGY
Sbjct: 397 AERPSYLTDSGAISLNEPSLGPKVNASSWLAVYPQGLKDTLMDIKRKYNDPIIYITENGY 456

Query: 244 FDYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           FDYD P+V +LI DKKR  ++  HLYYL +A+
Sbjct: 457 FDYDGPNVKELIDDKKRAEFYNDHLYYLHKAM 487

[Close]

BLAST of Lsi06G004940 vs. NCBI nr
Match: XP_038880765.1 (cyanogenic beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 458.4 bits (1178), Expect = 4.2e-125
Identity = 220/273 (80.59%), Postives = 237/273 (86.81%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DYVDLCF+HFGDRVKHWITLNEPNLFA+NGY  G FAPGRCS WVLNNCCDGNSS 
Sbjct: 176 NDFEDYVDLCFKHFGDRVKHWITLNEPNLFAQNGYGIGTFAPGRCSSWVLNNCCDGNSST 235

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIVSH+EILAH  AVKLYR KYQ+ QKG+IGITVL NWYEP+SE+A DKEAAKRATE 
Sbjct: 236 EPYIVSHHEILAHAHAVKLYRMKYQKIQKGIIGITVLENWYEPYSESAADKEAAKRATEF 295

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               FYNPIVHGDYP SMR+RVKDRLP FTEEEK+LIKGSFDFLGLNYYTAAYAIDDSNT
Sbjct: 296 FSGWFYNPIVHGDYPESMRLRVKDRLPTFTEEEKNLIKGSFDFLGLNYYTAAYAIDDSNT 355

Query: 183 HVLYPSCLTDSGV----------NASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS LTDSGV          NASSWLSVYP GL+KLLV MKN+YNNP+IYITENG+F
Sbjct: 356 HVLYPSYLTDSGVNTSISLGPKLNASSWLSVYPPGLEKLLVTMKNNYNNPNIYITENGFF 415

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAIVT 261
           DYDDP+V KLICDKKRTLYHI HLY+L +AI T
Sbjct: 416 DYDDPNVGKLICDKKRTLYHITHLYHLHRAIFT 448

[Close]

BLAST of Lsi06G004940 vs. NCBI nr
Match: XP_023516697.1 (beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.6 bits (1098), Expect = 7.9e-116
Identity = 200/271 (73.80%), Postives = 225/271 (83.03%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DYVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNCCDG+SS 
Sbjct: 174 NDFEDYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWILNNCCDGDSSK 233

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAVK+YRTKYQ  QKGVIGITV+ NWYEP+S T  DKEAAKRA E 
Sbjct: 234 EPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVIGNWYEPYSNTFADKEAAKRAIEF 293

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVKDRLP FT++E  LIK S+DFLGLNYYTAAYAIDDS+ 
Sbjct: 294 FSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLIKCSYDFLGLNYYTAAYAIDDSSA 353

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS  TDSG          VNASSWLSVYP GL+KLL+ MKNDYNNP IYITENG+F
Sbjct: 354 HVLYPSWRTDSGVNTSISLGPKVNASSWLSVYPKGLQKLLITMKNDYNNPSIYITENGFF 413

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           DYDDP   KL+CD+KRT YH+ HLY+LL+A+
Sbjct: 414 DYDDPSARKLLCDRKRTFYHMGHLYFLLEAM 444

[Close]

BLAST of Lsi06G004940 vs. NCBI nr
Match: XP_022988084.1 (cyanogenic beta-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 425.6 bits (1093), Expect = 3.0e-115
Identity = 198/272 (72.79%), Postives = 226/272 (83.09%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           DDF  YVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNCCDG+SS 
Sbjct: 174 DDFEGYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWMLNNCCDGDSSK 233

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAV +YRTKYQ  QKGVIGITV+ NWYEP+S+T  D+EAAKRA E 
Sbjct: 234 EPYIVGHNQILAHARAVDVYRTKYQRTQKGVIGITVIGNWYEPYSDTCADEEAAKRAIEF 293

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVK+RLP FT++E  LIKGS+DFLGLNYYTAAYAIDDS+ 
Sbjct: 294 FSGWFFNPIVHGDYPQSMRLRVKERLPTFTQDEATLIKGSYDFLGLNYYTAAYAIDDSSA 353

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS LTDSG          VNASSWLS+YP GLKKLL+ MKNDYNNP IYITENG+F
Sbjct: 354 HVLYPSWLTDSGVNTSISLGPKVNASSWLSIYPEGLKKLLITMKNDYNNPSIYITENGFF 413

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAIV 260
           DYDDP   KL+CD+KRT YH+ HLY+L +A++
Sbjct: 414 DYDDPSARKLLCDRKRTRYHMGHLYFLHEAMM 445

[Close]

BLAST of Lsi06G004940 vs. NCBI nr
Match: XP_022960679.1 (beta-glucosidase 12-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 416.4 bits (1069), Expect = 1.8e-112
Identity = 196/271 (72.32%), Postives = 222/271 (81.92%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DYVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNC DG+SS 
Sbjct: 174 NDFEDYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWILNNCYDGDSSK 233

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAVK+YRTKYQ  QKGVIGITV+ NWYEP+S T  D+EAAKRA E 
Sbjct: 234 EPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVIGNWYEPYSNTFADEEAAKRAIEF 293

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVKDRLP FT++E  LIK S+DFLGLNYYTAAYAIDDS+ 
Sbjct: 294 FSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLIKCSYDFLGLNYYTAAYAIDDSSA 353

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS  TDSG          VNASSWLSVYP GL+KLL+ MKNDY+NP IYITENG+F
Sbjct: 354 HVLYPSWRTDSGVNTSISLGPKVNASSWLSVYPKGLQKLLITMKNDYDNPSIYITENGFF 413

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           DYDDP   KL+CD+KR  YH+ HLY+L QA+
Sbjct: 414 DYDDPSARKLLCDRKRIFYHMGHLYFLRQAM 444

[Close]

BLAST of Lsi06G004940 vs. NCBI nr
Match: XP_022960680.1 (beta-glucosidase 12-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 416.4 bits (1069), Expect = 1.8e-112
Identity = 196/271 (72.32%), Postives = 222/271 (81.92%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DYVD+CF  FGDRVKHWITLNEPN+FA NGYA G FAPGRCSRW+LNNC DG+SS 
Sbjct: 77  NDFEDYVDVCFNQFGDRVKHWITLNEPNMFAINGYAQGTFAPGRCSRWILNNCYDGDSSK 136

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN+ILAH RAVK+YRTKYQ  QKGVIGITV+ NWYEP+S T  D+EAAKRA E 
Sbjct: 137 EPYIVGHNQILAHARAVKVYRTKYQRTQKGVIGITVIGNWYEPYSNTFADEEAAKRAIEF 196

Query: 123 ----FYNPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAIDDSNT 182
               F+NPIVHGDYP+SMR+RVKDRLP FT++E  LIK S+DFLGLNYYTAAYAIDDS+ 
Sbjct: 197 FSGWFFNPIVHGDYPQSMRLRVKDRLPTFTQDEATLIKCSYDFLGLNYYTAAYAIDDSSA 256

Query: 183 HVLYPSCLTDSG----------VNASSWLSVYPLGLKKLLVNMKNDYNNPDIYITENGYF 242
           HVLYPS  TDSG          VNASSWLSVYP GL+KLL+ MKNDY+NP IYITENG+F
Sbjct: 257 HVLYPSWRTDSGVNTSISLGPKVNASSWLSVYPKGLQKLLITMKNDYDNPSIYITENGFF 316

Query: 243 DYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           DYDDP   KL+CD+KR  YH+ HLY+L QA+
Sbjct: 317 DYDDPSARKLLCDRKRIFYHMGHLYFLRQAM 347

[Close]

BLAST of Lsi06G004940 vs. TAIR 10
Match: AT2G44480.1 (beta glucosidase 17 )

HSP 1 Score: 235.3 bits (599), Expect = 5.4e-62
Identity = 126/279 (45.16%), Postives = 174/279 (62.37%), Query Frame = 0


Query: 4   DFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSIE 63
           DFV+YVD+CF+ FGDRVK WIT+NEPN+FA  GY  G  APGRCS +V  NC  GNS+ E
Sbjct: 180 DFVEYVDICFKEFGDRVKEWITINEPNMFAVLGYNVGNIAPGRCSSYV-QNCTVGNSATE 239

Query: 64  PYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATEFY 123
           PY+V+H  IL+H   V+LYR KYQ    G IG+T+   W  P   T   +EAAKRA +F+
Sbjct: 240 PYLVAHYLILSHAATVQLYREKYQSFHGGTIGMTIQTYWMIPKYNTPACREAAKRALDFF 299

Query: 124 -----NPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAID---DS 183
                +PI +GDYP++MR  V +RLPKFT+++  +++GSFDF GLNYYT+ Y  D    +
Sbjct: 300 FGWFADPITYGDYPKTMRELVGNRLPKFTKKQSKMVRGSFDFFGLNYYTSRYVEDVMFYA 359

Query: 184 NTHVLYPSCLTDSGVN--------------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYI 243
           NT++ Y    TDS VN              ++ WL + P G + +L+ +K+ + NP I +
Sbjct: 360 NTNLSY---TTDSRVNQTTEKNGVPVGEPTSADWLFICPEGFQDVLLYIKSKFQNPVILV 419

Query: 244 TENGYFDYDDP--DVDKLICDKKRTLYHIAHLYYLLQAI 259
           TENG    +D    V+  + D+ +  YH  HL  LL+A+
Sbjct: 420 TENGMPSENDKSLSVNIALNDEAKIKYHQLHLTALLEAV 454

[Close]

BLAST of Lsi06G004940 vs. TAIR 10
Match: AT2G44480.2 (beta glucosidase 17 )

HSP 1 Score: 235.3 bits (599), Expect = 5.4e-62
Identity = 126/279 (45.16%), Postives = 174/279 (62.37%), Query Frame = 0


Query: 4   DFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSIE 63
           DFV+YVD+CF+ FGDRVK WIT+NEPN+FA  GY  G  APGRCS +V  NC  GNS+ E
Sbjct: 78  DFVEYVDICFKEFGDRVKEWITINEPNMFAVLGYNVGNIAPGRCSSYV-QNCTVGNSATE 137

Query: 64  PYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATEFY 123
           PY+V+H  IL+H   V+LYR KYQ    G IG+T+   W  P   T   +EAAKRA +F+
Sbjct: 138 PYLVAHYLILSHAATVQLYREKYQSFHGGTIGMTIQTYWMIPKYNTPACREAAKRALDFF 197

Query: 124 -----NPIVHGDYPRSMRIRVKDRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAID---DS 183
                +PI +GDYP++MR  V +RLPKFT+++  +++GSFDF GLNYYT+ Y  D    +
Sbjct: 198 FGWFADPITYGDYPKTMRELVGNRLPKFTKKQSKMVRGSFDFFGLNYYTSRYVEDVMFYA 257

Query: 184 NTHVLYPSCLTDSGVN--------------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYI 243
           NT++ Y    TDS VN              ++ WL + P G + +L+ +K+ + NP I +
Sbjct: 258 NTNLSY---TTDSRVNQTTEKNGVPVGEPTSADWLFICPEGFQDVLLYIKSKFQNPVILV 317

Query: 244 TENGYFDYDDP--DVDKLICDKKRTLYHIAHLYYLLQAI 259
           TENG    +D    V+  + D+ +  YH  HL  LL+A+
Sbjct: 318 TENGMPSENDKSLSVNIALNDEAKIKYHQLHLTALLEAV 352

[Close]

BLAST of Lsi06G004940 vs. TAIR 10
Match: AT5G44640.1 (beta glucosidase 13 )

HSP 1 Score: 235.3 bits (599), Expect = 5.4e-62
Identity = 123/275 (44.73%), Postives = 168/275 (61.09%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DY D+CF++FGDRVKHW+TLNEP    + GY  GV APGRCS++   NC  GN + 
Sbjct: 175 NDFRDYADICFKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGAT 234

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN ILAHG AVK+YR KY+  QKG +GI + A W  P++E+A+D+ AA RA   
Sbjct: 235 EPYIVGHNLILAHGEAVKVYREKYKASQKGQVGIALNAGWNLPYTESAEDRLAAARAMAF 294

Query: 123 ----FYNPIVHGDYPRSMRIRVKD-RLPKFTEEEKDLIKGSFDFLGLNYYTAAYAID--- 182
               F  P+V G YP  M   VKD RLP FT ++  ++KGS+DF+G+NYY+++YA D   
Sbjct: 295 TFDYFMEPLVTGKYPVDMVNNVKDGRLPTFTAKQSKMLKGSYDFIGINYYSSSYAKDVPC 354

Query: 183 -DSNTHVLYPSCLTDSGVN---------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYITE 242
              N  +    C + +G           AS WL +YP G++ LL+  K  + +P +YITE
Sbjct: 355 SSENVTLFSDPCASVTGEREGVPIGPKAASDWLLIYPKGIRDLLLYAKYKFKDPVMYITE 414

Query: 243 NGYFDYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           NG  +     +D  + D +R  Y+  HL  +  AI
Sbjct: 415 NGRDEASTGKID--LKDSERIDYYAQHLKMVQDAI 447

[Close]

BLAST of Lsi06G004940 vs. TAIR 10
Match: AT5G42260.1 (beta glucosidase 12 )

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-61
Identity = 123/275 (44.73%), Postives = 166/275 (60.36%), Query Frame = 0


Query: 3   DDFVDYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCCDGNSSI 62
           +DF DY D+CF++FGDRVKHW+TLNEP    + GY  GV APGRCS++   NC  GN + 
Sbjct: 175 NDFRDYADICFKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGAT 234

Query: 63  EPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAAKRATE- 122
           EPYIV HN ILAHG AVK+YR KY+  QKG +GI + A W  P+SE+A+D+ AA RA   
Sbjct: 235 EPYIVGHNLILAHGEAVKVYREKYKASQKGQVGIALNAGWNLPYSESAEDRLAAARAMAF 294

Query: 123 ----FYNPIVHGDYPRSMRIRVK-DRLPKFTEEEKDLIKGSFDFLGLNYYTAAYAID--- 182
               F  P+V G YP  M   VK  RLP FT ++  ++KGS+DF+G NYY+++YA D   
Sbjct: 295 TFDYFMEPLVTGKYPIDMVNYVKGGRLPTFTAKQSKMLKGSYDFIGRNYYSSSYAKDVPC 354

Query: 183 -DSNTHVLYPSCLTDSGVN---------ASSWLSVYPLGLKKLLVNMKNDYNNPDIYITE 242
              N  +    C + +G           AS WL +YP G++ LL+  K  + +P +YITE
Sbjct: 355 SSENVTLFSDPCASVTGEREGVPIGPKAASDWLLIYPKGIRDLLLYAKYKFKDPVMYITE 414

Query: 243 NGYFDYDDPDVDKLICDKKRTLYHIAHLYYLLQAI 259
           NG  +     +D  + D +R  Y+  HL  +  AI
Sbjct: 415 NGRDEASTGKID--LKDSERIDYYAQHLKMVQDAI 447

[Close]

BLAST of Lsi06G004940 vs. TAIR 10
Match: AT3G60130.1 (beta glucosidase 16 )

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-60
Identity = 128/282 (45.39%), Postives = 171/282 (60.64%), Query Frame = 0


Query: 1   MGDDFV----DYVDLCFRHFGDRVKHWITLNEPNLFARNGYATGVFAPGRCSRWVLNNCC 60
           +GD+FV    DY +LCF+ FGDRVK W TLNEP      GY TG  APGRCS +   +C 
Sbjct: 168 LGDEFVNDFRDYAELCFQKFGDRVKQWTTLNEPYTMVHEGYITGQKAPGRCSNFYKPDCL 227

Query: 61  DGNSSIEPYIVSHNEILAHGRAVKLYRTKYQEKQKGVIGITVLANWYEPHSETAKDKEAA 120
            G+++ EPYIV HN +LAHG AVK+YR KYQ  QKG IGI +   W+ P+S++  D+ AA
Sbjct: 228 GGDAATEPYIVGHNLLLAHGVAVKVYREKYQATQKGEIGIALNTAWHYPYSDSYADRLAA 287

Query: 121 KRATE-----FYNPIVHGDYPRSMRIRVKD-RLPKFTEEEKDLIKGSFDFLGLNYYTAAY 180
            RAT      F  PIV+G YP  M   VKD RLP FT EE +++KGS+DF+G+NYY++ Y
Sbjct: 288 TRATAFTFDYFMEPIVYGRYPIEMVSHVKDGRLPTFTPEESEMLKGSYDFIGVNYYSSLY 347

Query: 181 AID----DSNTHVLYPSCLTDSG---------VNASSWLSVYPLGLKKLLVNMKNDYNNP 240
           A D      N  +   SC++  G            S WL +YP G++ LL++ K  YN+P
Sbjct: 348 AKDVPCATENITMTTDSCVSLVGERNGVPIGPAAGSDWLLIYPKGIRDLLLHAKFRYNDP 407

Query: 241 DIYITENGYFDYDDPDVDKL-ICDKKRTLYHIAHLYYLLQAI 259
            +YITENG    D+ ++ K+ + D  R  Y+  HL  +  AI
Sbjct: 408 VLYITENG---VDEANIGKIFLNDDLRIDYYAHHLKMVSDAI 446

The following BLAST results are available for this feature:

BLAST of Lsi06G004940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B8AVF0	2.2e-68	47.29	Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1	[more]
Q7XKV4	2.9e-68	47.29	Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2	[more]
P26205	1.9e-67	51.65	Cyanogenic beta-glucosidase (Fragment) OS=Trifolium repens OX=3899 GN=LI PE=1 SV...	[more]
Q5Z9Z0	2.1e-66	46.57	Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1	[more]
A3RF67	2.7e-66	46.21	Isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase OS=Dalbergia nigrescens...	[more]

BLAST of Lsi06G004940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKM7	1.5e-115	72.79	cyanogenic beta-glucosidase-like OS=Cucurbita maxima OX=3661 GN=LOC111485436 PE=...	[more]
A0A6J1HBR5	8.8e-113	72.32	beta-glucosidase 12-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146139...	[more]
A0A6J1H9S5	8.8e-113	72.32	beta-glucosidase 12-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11146139...	[more]
A0A6J1JG58	3.7e-87	59.19	beta-glucosidase 24-like OS=Cucurbita maxima OX=3661 GN=LOC111485420 PE=3 SV=1	[more]
A0A6J1H894	4.9e-87	59.56	beta-glucosidase 24-like OS=Cucurbita moschata OX=3662 GN=LOC111461391 PE=3 SV=1	[more]

BLAST of Lsi06G004940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880765.1	4.2e-125	80.59	cyanogenic beta-glucosidase-like [Benincasa hispida]	[more]
XP_023516697.1	7.9e-116	73.80	beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022988084.1	3.0e-115	72.79	cyanogenic beta-glucosidase-like [Cucurbita maxima]	[more]
XP_022960679.1	1.8e-112	72.32	beta-glucosidase 12-like isoform X1 [Cucurbita moschata]	[more]
XP_022960680.1	1.8e-112	72.32	beta-glucosidase 12-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Lsi06G004940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44480.1	5.4e-62	45.16	beta glucosidase 17	[more]
AT2G44480.2	5.4e-62	45.16	beta glucosidase 17	[more]
AT5G44640.1	5.4e-62	44.73	beta glucosidase 13	[more]
AT5G42260.1	7.8e-61	44.73	beta glucosidase 12	[more]
AT3G60130.1	1.0e-60	45.39	beta glucosidase 16	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001360	Glycoside hydrolase family 1	PRINTS	PR00131	GLHYDRLASE1	coord: 157..171 score: 47.64 coord: 219..227 score: 60.59 coord: 240..251 score: 39.67
IPR001360	Glycoside hydrolase family 1	PFAM	PF00232	Glyco_hydro_1	coord: 3..258 e-value: 7.5E-57 score: 192.7
IPR001360	Glycoside hydrolase family 1	PANTHER	PTHR10353	GLYCOSYL HYDROLASE	coord: 3..258
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..260 e-value: 1.0E-91 score: 309.7
None	No IPR available	PANTHER	PTHR10353:SF272	SUBFAMILY NOT NAMED	coord: 3..258
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 3..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G004940.1	Lsi06G004940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds