Lsi06G004600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G004600
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	1,3-beta-glucan synthase
Location	chr06: 5158570 .. 5159729 (-)
RNA-Seq Expression	Lsi06G004600
Synteny	Lsi06G004600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GATATGCTTGGTAGAAGTCGCCATCAAGGGAAAACATCATTTGTTGAGCATCGGACTTTTCTTCATCTTTATCGTAGTTTCCATCGTTTATGGATATTTTTGGTTATGATGTTCCAGGTATATGGCCTTTTTAAATGCTCTTTGTTATCCTAGTCCTATTAACTAAAATTTGCTAACCTGGTCTTGATGCTTGGGAAAATTGTTTTGTTTTTGTATATGTGGTGTAGCCTAAATGACAAAAGAGATAATGCTCCCAGAAATTTAACCCAAAGGATTTTATTACACTTCATAAGTGTTCAATACAAGAGACTAGGATTTCTGCATTTTCATATATTTAATTATTATTTATCACATTGTTAATCTTACAGGCTCTGACCATTGTTGCATTTAACAATGGAAGCTTTAATATGAAGACACTGCTGGACGTTCTGAGTATTGGTCCGACATATGTTCTAATGAAGTTAATTGAGAGTATGTGCCGTTCTAGTCATTTCTACTGAAAGTGTTTGAACCTGATTTGTTCTATTATCTTGTGTGAAATAGATTGAAATATTTAACTGCATGGACTTCATCCAGGTGTTCTAGACATCCTAATGATGTATGGTGCATACCCTACTTCAAGGCGTCTTGCTGTTTCTCGGATTTTTCTTCGTTTTCTTTGGTTCAGTATTGCATCAGCTTTGATAACATTTCTTTATGTGTATGTATTCTTGCTTTAATGTTCTCGCTGTCTTGTTTTTTTCACAAGACGTTTAGTGTTGTTTTTATCGATTCCCTTACAGGAAAGCTCTTCAGGAGGGGAGTAAACCAAATGCAGAAAGAGTGATGTTCAGACTATATGTAATTGTGATTGGAATATATGGTGGTGTACAGCTTTGTCTCAGTATCCTCATGCATATACCTGCTTGCCGTCTTTTAATGAATCAATGTGATTGCTGGCCTCTTGTTCGCTTTTTCAAGTGGATGTGTCAGGTTTCACCCTCTTATACTTGCTTTATTTCTTCTTTTCTACTTCTAATGTGGCCTTCTCAATTTCAGTTGACACGCGATATTTTGTTAAATCTATACTTTCTCTTGATGCTGAATTTTTTTTTTTTTAATAGGAACGGTACTATGTTGGACGTGGAATGTATGAGAGGACTACTGACTTTATCAAGTAA

mRNA sequence

GATATGCTTGGTAGAAGTCGCCATCAAGGGAAAACATCATTTGTTGAGCATCGGACTTTTCTTCATCTTTATCGTAGTTTCCATCGTTTATGGATATTTTTGGTTATGATGTTCCAGGCTCTGACCATTGTTGCATTTAACAATGGAAGCTTTAATATGAAGACACTGCTGGACGTTCTGAGTATTGGTCCGACATATGTTCTAATGAAGTTAATTGAGAGTGTTCTAGACATCCTAATGATGTATGGTGCATACCCTACTTCAAGGCGTCTTGCTGTTTCTCGGATTTTTCTTCGTTTTCTTTGGTTCAGTATTGCATCAGCTTTGATAACATTTCTTTATGTGAAAGCTCTTCAGGAGGGGAGTAAACCAAATGCAGAAAGAGTGATGTTCAGACTATATGTAATTGTGATTGGAATATATGGTGGTGTACAGCTTTGTCTCAGTATCCTCATGCATATACCTGCTTGCCGTCTTTTAATGAATCAATGTGATTGCTGGCCTCTTGTTCGCTTTTTCAAGTGGATGTGTCAGGAACGGTACTATGTTGGACGTGGAATGTATGAGAGGACTACTGACTTTATCAAGTAA

Coding sequence (CDS)

GATATGCTTGGTAGAAGTCGCCATCAAGGGAAAACATCATTTGTTGAGCATCGGACTTTTCTTCATCTTTATCGTAGTTTCCATCGTTTATGGATATTTTTGGTTATGATGTTCCAGGCTCTGACCATTGTTGCATTTAACAATGGAAGCTTTAATATGAAGACACTGCTGGACGTTCTGAGTATTGGTCCGACATATGTTCTAATGAAGTTAATTGAGAGTGTTCTAGACATCCTAATGATGTATGGTGCATACCCTACTTCAAGGCGTCTTGCTGTTTCTCGGATTTTTCTTCGTTTTCTTTGGTTCAGTATTGCATCAGCTTTGATAACATTTCTTTATGTGAAAGCTCTTCAGGAGGGGAGTAAACCAAATGCAGAAAGAGTGATGTTCAGACTATATGTAATTGTGATTGGAATATATGGTGGTGTACAGCTTTGTCTCAGTATCCTCATGCATATACCTGCTTGCCGTCTTTTAATGAATCAATGTGATTGCTGGCCTCTTGTTCGCTTTTTCAAGTGGATGTGTCAGGAACGGTACTATGTTGGACGTGGAATGTATGAGAGGACTACTGACTTTATCAAGTAA

Protein sequence

DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLSIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEGSKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERYYVGRGMYERTTDFIK

Homology

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BLAST of Lsi06G004600 vs. ExPASy Swiss-Prot
Match: Q9SFU6 (Callose synthase 9 OS=Arabidopsis thaliana OX=3702 GN=CALS9 PE=2 SV=2)

HSP 1 Score: 265.8 bits (678), Expect = 3.9e-70
Identity = 131/194 (67.53%), Postives = 160/194 (82.47%), Query Frame = 0


Query: 4   GRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSF-NMKTLLDVLSI 63
           GR++H+GKTSFVEHRTFLHLY SFHRLWIFL MMFQAL I+AFN     + KTLL +LS+
Sbjct: 468 GRAKHRGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQALAIIAFNKDDLTSRKTLLQILSL 527

Query: 64  GPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEGS 123
           GPT+V+MK  ESVL+++MMYGAY T+RRLAVSRIFLRF+WF +AS  I+FLYVK+L+   
Sbjct: 528 GPTFVVMKFSESVLEVIMMYGAYSTTRRLAVSRIFLRFIWFGLASVFISFLYVKSLK--- 587

Query: 124 KPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERYY 183
            PN++  + +LY+IVI IYGGVQ   SILM IP C  + N+CD WP++RFFKWM QER+Y
Sbjct: 588 APNSDSPIVQLYLIVIAIYGGVQFFFSILMRIPTCHNIANKCDRWPVIRFFKWMRQERHY 647

Query: 184 VGRGMYERTTDFIK 197
           VGRGMYERT+DFIK
Sbjct: 648 VGRGMYERTSDFIK 658

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BLAST of Lsi06G004600 vs. ExPASy Swiss-Prot
Match: Q9SJM0 (Callose synthase 10 OS=Arabidopsis thaliana OX=3702 GN=CALS10 PE=2 SV=5)

HSP 1 Score: 198.7 bits (504), Expect = 5.9e-50
Identity = 95/193 (49.22%), Postives = 135/193 (69.95%), Query Frame = 0


Query: 4   GRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLSIG 63
           GR R   K+SFVEHRT+LHL+RSF RLWIF+ +MFQ+LTI+AF N   N++T   +LS G
Sbjct: 470 GRKR-TAKSSFVEHRTYLHLFRSFIRLWIFMFIMFQSLTIIAFRNEHLNIETFKILLSAG 529

Query: 64  PTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEGSK 123
           PTY +M  IE +LD+++MYGAY  +R +A+SR+ +RFLW+ + SA + + YVK L E +K
Sbjct: 530 PTYAIMNFIECLLDVVLMYGAYSMARGMAISRLVIRFLWWGLGSAFVVYYYVKVLDERNK 589

Query: 124 PNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERYYV 183
           PN     F LY++V+G Y  V+L   +L+ +PAC  L    D     +FFKW+ QERY+V
Sbjct: 590 PNQNEFFFHLYILVLGCYAAVRLIFGLLVKLPACHALSEMSD-QSFFQFFKWIYQERYFV 649

Query: 184 GRGMYERTTDFIK 197
           GRG++E  +D+ +
Sbjct: 650 GRGLFENLSDYCR 660

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BLAST of Lsi06G004600 vs. ExPASy Swiss-Prot
Match: Q9SL03 (Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3)

HSP 1 Score: 84.3 bits (207), Expect = 1.6e-15
Identity = 64/208 (30.77%), Postives = 102/208 (49.04%), Query Frame = 0


Query: 7   RHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFN-----NGSFNMKTLLDVLS 66
           R  GK +FVE R+F H++RSF R+W F ++  QA+ I+A+N     +G F     L VLS
Sbjct: 459 RWMGKVNFVEIRSFWHIFRSFDRMWSFYILSLQAMIIIAWNGSGKLSGIFQGDVFLKVLS 518

Query: 67  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASAL------ITFLYV 126
           I  T  ++KL ++VLDI + +     SR      + LRF++ ++A+A+      +T+ Y 
Sbjct: 519 IFITAAILKLAQAVLDIALSW----KSRHSMSFHVKLRFIFKAVAAAIWVVLMPLTYAYS 578

Query: 127 KALQEGSKPNA-------ERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWP 186
                G            +      ++IVI IY    +  ++L   P  R  + + D + 
Sbjct: 579 WKTPSGFAETIKNWFGGHQNSSPSFFIIVILIYLSPNMLSTLLFAFPFIRRYLERSD-YK 638

Query: 187 LVRFFKWMCQERYYVGRGMYERTTDFIK 197
           +V    W  Q R Y+GRGM+E      K
Sbjct: 639 IVMLMMWWSQPRLYIGRGMHESALSLFK 661

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BLAST of Lsi06G004600 vs. ExPASy Swiss-Prot
Match: Q9SHJ3 (Callose synthase 7 OS=Arabidopsis thaliana OX=3702 GN=CALS7 PE=3 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 2.1e-15
Identity = 58/201 (28.86%), Postives = 105/201 (52.24%), Query Frame = 0


Query: 1   DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFN-NGS----FNMKT 60
           D +   + + KT+FVE RTF +LYRSF R+W+FLV+  Q + IVA++ +GS    F    
Sbjct: 473 DQVSHGKRKPKTNFVEARTFWNLYRSFDRMWMFLVLSLQTMIIVAWHPSGSILAIFTEDV 532

Query: 61  LLDVLSIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYV 120
             +VL+I  T   + L+++ LD+++ +GA+ + +   + R   +FL  ++ + ++   Y 
Sbjct: 533 FRNVLTIFITSAFLNLLQATLDLVLSFGAWKSLKFSQIMRYITKFLMAAMWAIMLPITYS 592

Query: 121 KALQEG-------SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWP 180
           K++Q         S      +   LY   I +Y    +  ++   +P  R +M + +   
Sbjct: 593 KSVQNPTGLIKFFSSWVGSWLHRSLYDYAIALYVLPNILAAVFFLLPPLRRIMERSN-MR 652

Query: 181 LVRFFKWMCQERYYVGRGMYE 190
           +V    W  Q + Y+GRGM+E
Sbjct: 653 IVTLIMWWAQPKLYIGRGMHE 672

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BLAST of Lsi06G004600 vs. ExPASy Swiss-Prot
Match: Q9LYS6 (Putative callose synthase 6 OS=Arabidopsis thaliana OX=3702 GN=CALS6 PE=3 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 4.0e-14
Identity = 61/194 (31.44%), Postives = 97/194 (50.00%), Query Frame = 0


Query: 11  KTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFN-NGS----FNMKTLLDVLSIGPT 70
           KT+FVE RTF +L+R F R+WIFLVM FQA+ IV ++ +GS    F+      VL+I  T
Sbjct: 474 KTNFVEVRTFWNLFRDFDRMWIFLVMAFQAMVIVGWHGSGSLGDIFDKDVFKTVLTIFIT 533

Query: 71  YVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEGSKPN 130
              + L+++ LDI++ + A+   +   + R  L+F    + + L+   Y K++Q   +P 
Sbjct: 534 SAYLTLLQAALDIILNFNAWKNFKFSQILRYLLKFAVAFMWAVLLPIAYSKSVQ---RPT 593

Query: 131 AERVMF----------RLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKW 190
                F            Y   +  Y    +  ++L  +P  R  M   D  P ++   W
Sbjct: 594 GVVKFFSTWTGDWKDQSFYTYAVSFYVLPNILAALLFLVPPFRRAMECSDMRP-IKVIMW 653

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BLAST of Lsi06G004600 vs. ExPASy TrEMBL
Match: A0A0A0LZH8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G604975 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.1e-94
Identity = 179/195 (91.79%), Postives = 185/195 (94.87%), Query Frame = 0


Query: 2   MLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLS 61
           MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTI+AFNNGSFNMK LL+VLS
Sbjct: 16  MLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIIAFNNGSFNMKMLLEVLS 75

Query: 62  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEG 121
           +GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRF+WFSIASA ITFLYVKALQEG
Sbjct: 76  LGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFIWFSIASASITFLYVKALQEG 135

Query: 122 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERY 181
           SKPNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRFFKWM QERY
Sbjct: 136 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFFKWMRQERY 195

Query: 182 YVGRGMYERTTDFIK 197
           YVGRGMYERTTDFIK
Sbjct: 196 YVGRGMYERTTDFIK 210

[Close]

BLAST of Lsi06G004600 vs. ExPASy TrEMBL
Match: A0A1S4DYC5 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103492488 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.9e-94
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 2   MLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLS 61
           MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTI+AFNNGSFNMKTLL+VLS
Sbjct: 464 MLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIIAFNNGSFNMKTLLEVLS 523

Query: 62  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEG 121
           +GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRF+WFSIASA ITFLYVKALQEG
Sbjct: 524 LGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFIWFSIASASITFLYVKALQEG 583

Query: 122 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERY 181
           S PNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRF KWM QERY
Sbjct: 584 SGPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFIKWMRQERY 643

Query: 182 YVGRGMYERTTDFIK 197
           YVGRGMYERTTDFIK
Sbjct: 644 YVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. ExPASy TrEMBL
Match: A0A5A7TJV3 (Callose synthase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00250 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.9e-94
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 2   MLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLS 61
           MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTI+AFNNGSFNMKTLL+VLS
Sbjct: 611 MLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIIAFNNGSFNMKTLLEVLS 670

Query: 62  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEG 121
           +GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRF+WFSIASA ITFLYVKALQEG
Sbjct: 671 LGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFIWFSIASASITFLYVKALQEG 730

Query: 122 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERY 181
           S PNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRF KWM QERY
Sbjct: 731 SGPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFIKWMRQERY 790

Query: 182 YVGRGMYERTTDFIK 197
           YVGRGMYERTTDFIK
Sbjct: 791 YVGRGMYERTTDFIK 805

[Close]

BLAST of Lsi06G004600 vs. ExPASy TrEMBL
Match: A0A6J1HN98 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111466194 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.2e-92
Identity = 177/196 (90.31%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVL 60
           +MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTIVAFNNG+FNMKTLL+VL
Sbjct: 463 NMLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIVAFNNGNFNMKTLLEVL 522

Query: 61  SIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQE 120
           S+GPT+V+MK IESVLDILMMYGAY TSRRLA+SRIFLRFLWFSIASA ITFLYVKALQE
Sbjct: 523 SLGPTFVVMKFIESVLDILMMYGAYSTSRRLAISRIFLRFLWFSIASASITFLYVKALQE 582

Query: 121 GSKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQER 180
           GS PNAERVMFRLYVIVIGIYGGVQL LSILM IPA  LL NQCD WPLVRFFKWM QER
Sbjct: 583 GSNPNAERVMFRLYVIVIGIYGGVQLFLSILMRIPAFHLLTNQCDRWPLVRFFKWMRQER 642

Query: 181 YYVGRGMYERTTDFIK 197
           YYVGRGMYERTTDFIK
Sbjct: 643 YYVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. ExPASy TrEMBL
Match: A0A6J1EP57 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111436119 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.2e-92
Identity = 177/196 (90.31%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVL 60
           +MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTIVAFNNG+FNMKTLL+VL
Sbjct: 463 NMLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIVAFNNGNFNMKTLLEVL 522

Query: 61  SIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQE 120
           S+GPT+V+MK IESVLDILMMYGAY TSRRLA+SRIFLRFLWFSIASA ITFLYVKALQE
Sbjct: 523 SLGPTFVVMKFIESVLDILMMYGAYSTSRRLAISRIFLRFLWFSIASASITFLYVKALQE 582

Query: 121 GSKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQER 180
           GS PNAERVMFRLYVIVIGIYGGVQL LSILM IPA  LL NQCD WPLVRFFKWM QER
Sbjct: 583 GSNPNAERVMFRLYVIVIGIYGGVQLFLSILMRIPAFHLLTNQCDRWPLVRFFKWMRQER 642

Query: 181 YYVGRGMYERTTDFIK 197
           YYVGRGMYERTTDFIK
Sbjct: 643 YYVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. NCBI nr
Match: XP_011660099.1 (callose synthase 9 [Cucumis sativus] >KAE8653490.1 hypothetical protein Csa_007250 [Cucumis sativus])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 179/195 (91.79%), Postives = 185/195 (94.87%), Query Frame = 0


Query: 2   MLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLS 61
           MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTI+AFNNGSFNMK LL+VLS
Sbjct: 464 MLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIIAFNNGSFNMKMLLEVLS 523

Query: 62  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEG 121
           +GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRF+WFSIASA ITFLYVKALQEG
Sbjct: 524 LGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFIWFSIASASITFLYVKALQEG 583

Query: 122 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERY 181
           SKPNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRFFKWM QERY
Sbjct: 584 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFFKWMRQERY 643

Query: 182 YVGRGMYERTTDFIK 197
           YVGRGMYERTTDFIK
Sbjct: 644 YVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. NCBI nr
Match: XP_038878486.1 (callose synthase 9-like [Benincasa hispida] >XP_038878487.1 callose synthase 9-like [Benincasa hispida])

HSP 1 Score: 354.4 bits (908), Expect = 6.4e-94
Identity = 179/196 (91.33%), Postives = 186/196 (94.90%), Query Frame = 0


Query: 1   DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVL 60
           +MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTIVAFN+GSFN+KTLL+VL
Sbjct: 463 NMLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIVAFNDGSFNIKTLLEVL 522

Query: 61  SIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQE 120
           S+GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRFLWFSIASA ITFLYVKALQE
Sbjct: 523 SLGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFLWFSIASASITFLYVKALQE 582

Query: 121 GSKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQER 180
           GSKPNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRF KWM QER
Sbjct: 583 GSKPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFIKWMRQER 642

Query: 181 YYVGRGMYERTTDFIK 197
           YYVGRGMYERTTDFIK
Sbjct: 643 YYVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. NCBI nr
Match: KAA0042246.1 (callose synthase 9 [Cucumis melo var. makuwa])

HSP 1 Score: 353.2 bits (905), Expect = 1.4e-93
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 2   MLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLS 61
           MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTI+AFNNGSFNMKTLL+VLS
Sbjct: 611 MLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIIAFNNGSFNMKTLLEVLS 670

Query: 62  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEG 121
           +GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRF+WFSIASA ITFLYVKALQEG
Sbjct: 671 LGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFIWFSIASASITFLYVKALQEG 730

Query: 122 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERY 181
           S PNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRF KWM QERY
Sbjct: 731 SGPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFIKWMRQERY 790

Query: 182 YVGRGMYERTTDFIK 197
           YVGRGMYERTTDFIK
Sbjct: 791 YVGRGMYERTTDFIK 805

[Close]

BLAST of Lsi06G004600 vs. NCBI nr
Match: XP_008451107.1 (PREDICTED: callose synthase 9 [Cucumis melo] >XP_016900973.1 PREDICTED: callose synthase 9 [Cucumis melo])

HSP 1 Score: 353.2 bits (905), Expect = 1.4e-93
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 2   MLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLS 61
           MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTI+AFNNGSFNMKTLL+VLS
Sbjct: 464 MLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIIAFNNGSFNMKTLLEVLS 523

Query: 62  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEG 121
           +GPT+V+MK IESVLDILMMYGAY TSRRLAVSRIFLRF+WFSIASA ITFLYVKALQEG
Sbjct: 524 LGPTFVVMKFIESVLDILMMYGAYSTSRRLAVSRIFLRFIWFSIASASITFLYVKALQEG 583

Query: 122 SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERY 181
           S PNAERVMFRLYVIVIGIYGGVQLCLSILM IPAC LL NQCD WPLVRF KWM QERY
Sbjct: 584 SGPNAERVMFRLYVIVIGIYGGVQLCLSILMRIPACHLLTNQCDRWPLVRFIKWMRQERY 643

Query: 182 YVGRGMYERTTDFIK 197
           YVGRGMYERTTDFIK
Sbjct: 644 YVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. NCBI nr
Match: XP_023531967.1 (callose synthase 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 348.2 bits (892), Expect = 4.6e-92
Identity = 177/196 (90.31%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVL 60
           +MLGRSRHQGKTSFVEHRTFLHLY SFHRLWIFLVMMFQALTIVAFNNG+FNMKTLL+VL
Sbjct: 463 NMLGRSRHQGKTSFVEHRTFLHLYHSFHRLWIFLVMMFQALTIVAFNNGNFNMKTLLEVL 522

Query: 61  SIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQE 120
           S+GPT+V+MK IESVLDILMMYGAY TSRRLA+SRIFLRFLWFSIASA ITFLYVKALQE
Sbjct: 523 SLGPTFVVMKFIESVLDILMMYGAYSTSRRLAISRIFLRFLWFSIASASITFLYVKALQE 582

Query: 121 GSKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQER 180
           GS PNAERVMFRLYVIVIGIYGGVQL LSILM IPA  LL NQCD WPLVRFFKWM QER
Sbjct: 583 GSNPNAERVMFRLYVIVIGIYGGVQLFLSILMRIPAFHLLTNQCDRWPLVRFFKWMRQER 642

Query: 181 YYVGRGMYERTTDFIK 197
           YYVGRGMYERTTDFIK
Sbjct: 643 YYVGRGMYERTTDFIK 658

[Close]

BLAST of Lsi06G004600 vs. TAIR 10
Match: AT3G07160.1 (glucan synthase-like 10 )

HSP 1 Score: 265.8 bits (678), Expect = 2.8e-71
Identity = 131/194 (67.53%), Postives = 160/194 (82.47%), Query Frame = 0


Query: 4   GRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSF-NMKTLLDVLSI 63
           GR++H+GKTSFVEHRTFLHLY SFHRLWIFL MMFQAL I+AFN     + KTLL +LS+
Sbjct: 468 GRAKHRGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQALAIIAFNKDDLTSRKTLLQILSL 527

Query: 64  GPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEGS 123
           GPT+V+MK  ESVL+++MMYGAY T+RRLAVSRIFLRF+WF +AS  I+FLYVK+L+   
Sbjct: 528 GPTFVVMKFSESVLEVIMMYGAYSTTRRLAVSRIFLRFIWFGLASVFISFLYVKSLK--- 587

Query: 124 KPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERYY 183
            PN++  + +LY+IVI IYGGVQ   SILM IP C  + N+CD WP++RFFKWM QER+Y
Sbjct: 588 APNSDSPIVQLYLIVIAIYGGVQFFFSILMRIPTCHNIANKCDRWPVIRFFKWMRQERHY 647

Query: 184 VGRGMYERTTDFIK 197
           VGRGMYERT+DFIK
Sbjct: 648 VGRGMYERTSDFIK 658

[Close]

BLAST of Lsi06G004600 vs. TAIR 10
Match: AT2G36850.1 (glucan synthase-like 8 )

HSP 1 Score: 198.7 bits (504), Expect = 4.2e-51
Identity = 95/193 (49.22%), Postives = 135/193 (69.95%), Query Frame = 0


Query: 4   GRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFNNGSFNMKTLLDVLSIG 63
           GR R   K+SFVEHRT+LHL+RSF RLWIF+ +MFQ+LTI+AF N   N++T   +LS G
Sbjct: 470 GRKR-TAKSSFVEHRTYLHLFRSFIRLWIFMFIMFQSLTIIAFRNEHLNIETFKILLSAG 529

Query: 64  PTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYVKALQEGSK 123
           PTY +M  IE +LD+++MYGAY  +R +A+SR+ +RFLW+ + SA + + YVK L E +K
Sbjct: 530 PTYAIMNFIECLLDVVLMYGAYSMARGMAISRLVIRFLWWGLGSAFVVYYYVKVLDERNK 589

Query: 124 PNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWPLVRFFKWMCQERYYV 183
           PN     F LY++V+G Y  V+L   +L+ +PAC  L    D     +FFKW+ QERY+V
Sbjct: 590 PNQNEFFFHLYILVLGCYAAVRLIFGLLVKLPACHALSEMSD-QSFFQFFKWIYQERYFV 649

Query: 184 GRGMYERTTDFIK 197
           GRG++E  +D+ +
Sbjct: 650 GRGLFENLSDYCR 660

[Close]

BLAST of Lsi06G004600 vs. TAIR 10
Match: AT2G31960.1 (glucan synthase-like 3 )

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-16
Identity = 64/208 (30.77%), Postives = 102/208 (49.04%), Query Frame = 0


Query: 7   RHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFN-----NGSFNMKTLLDVLS 66
           R  GK +FVE R+F H++RSF R+W F ++  QA+ I+A+N     +G F     L VLS
Sbjct: 459 RWMGKVNFVEIRSFWHIFRSFDRMWSFYILSLQAMIIIAWNGSGKLSGIFQGDVFLKVLS 518

Query: 67  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASAL------ITFLYV 126
           I  T  ++KL ++VLDI + +     SR      + LRF++ ++A+A+      +T+ Y 
Sbjct: 519 IFITAAILKLAQAVLDIALSW----KSRHSMSFHVKLRFIFKAVAAAIWVVLMPLTYAYS 578

Query: 127 KALQEGSKPNA-------ERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWP 186
                G            +      ++IVI IY    +  ++L   P  R  + + D + 
Sbjct: 579 WKTPSGFAETIKNWFGGHQNSSPSFFIIVILIYLSPNMLSTLLFAFPFIRRYLERSD-YK 638

Query: 187 LVRFFKWMCQERYYVGRGMYERTTDFIK 197
           +V    W  Q R Y+GRGM+E      K
Sbjct: 639 IVMLMMWWSQPRLYIGRGMHESALSLFK 661

[Close]

BLAST of Lsi06G004600 vs. TAIR 10
Match: AT2G31960.2 (glucan synthase-like 3 )

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-16
Identity = 64/208 (30.77%), Postives = 102/208 (49.04%), Query Frame = 0


Query: 7   RHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFN-----NGSFNMKTLLDVLS 66
           R  GK +FVE R+F H++RSF R+W F ++  QA+ I+A+N     +G F     L VLS
Sbjct: 459 RWMGKVNFVEIRSFWHIFRSFDRMWSFYILSLQAMIIIAWNGSGKLSGIFQGDVFLKVLS 518

Query: 67  IGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASAL------ITFLYV 126
           I  T  ++KL ++VLDI + +     SR      + LRF++ ++A+A+      +T+ Y 
Sbjct: 519 IFITAAILKLAQAVLDIALSW----KSRHSMSFHVKLRFIFKAVAAAIWVVLMPLTYAYS 578

Query: 127 KALQEGSKPNA-------ERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWP 186
                G            +      ++IVI IY    +  ++L   P  R  + + D + 
Sbjct: 579 WKTPSGFAETIKNWFGGHQNSSPSFFIIVILIYLSPNMLSTLLFAFPFIRRYLERSD-YK 638

Query: 187 LVRFFKWMCQERYYVGRGMYERTTDFIK 197
           +V    W  Q R Y+GRGM+E      K
Sbjct: 639 IVMLMMWWSQPRLYIGRGMHESALSLFK 661

[Close]

BLAST of Lsi06G004600 vs. TAIR 10
Match: AT1G06490.1 (glucan synthase-like 7 )

HSP 1 Score: 84.0 bits (206), Expect = 1.5e-16
Identity = 58/201 (28.86%), Postives = 105/201 (52.24%), Query Frame = 0


Query: 1   DMLGRSRHQGKTSFVEHRTFLHLYRSFHRLWIFLVMMFQALTIVAFN-NGS----FNMKT 60
           D +   + + KT+FVE RTF +LYRSF R+W+FLV+  Q + IVA++ +GS    F    
Sbjct: 473 DQVSHGKRKPKTNFVEARTFWNLYRSFDRMWMFLVLSLQTMIIVAWHPSGSILAIFTEDV 532

Query: 61  LLDVLSIGPTYVLMKLIESVLDILMMYGAYPTSRRLAVSRIFLRFLWFSIASALITFLYV 120
             +VL+I  T   + L+++ LD+++ +GA+ + +   + R   +FL  ++ + ++   Y 
Sbjct: 533 FRNVLTIFITSAFLNLLQATLDLVLSFGAWKSLKFSQIMRYITKFLMAAMWAIMLPITYS 592

Query: 121 KALQEG-------SKPNAERVMFRLYVIVIGIYGGVQLCLSILMHIPACRLLMNQCDCWP 180
           K++Q         S      +   LY   I +Y    +  ++   +P  R +M + +   
Sbjct: 593 KSVQNPTGLIKFFSSWVGSWLHRSLYDYAIALYVLPNILAAVFFLLPPLRRIMERSN-MR 652

Query: 181 LVRFFKWMCQERYYVGRGMYE 190
           +V    W  Q + Y+GRGM+E
Sbjct: 653 IVTLIMWWAQPKLYIGRGMHE 672

The following BLAST results are available for this feature:

BLAST of Lsi06G004600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SFU6	3.9e-70	67.53	Callose synthase 9 OS=Arabidopsis thaliana OX=3702 GN=CALS9 PE=2 SV=2	[more]
Q9SJM0	5.9e-50	49.22	Callose synthase 10 OS=Arabidopsis thaliana OX=3702 GN=CALS10 PE=2 SV=5	[more]
Q9SL03	1.6e-15	30.77	Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3	[more]
Q9SHJ3	2.1e-15	28.86	Callose synthase 7 OS=Arabidopsis thaliana OX=3702 GN=CALS7 PE=3 SV=3	[more]
Q9LYS6	4.0e-14	31.44	Putative callose synthase 6 OS=Arabidopsis thaliana OX=3702 GN=CALS6 PE=3 SV=2	[more]

BLAST of Lsi06G004600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LZH8	1.1e-94	91.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G604975 PE=4 SV=1	[more]
A0A1S4DYC5	6.9e-94	91.28	1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103492488 PE=3 SV=1	[more]
A0A5A7TJV3	6.9e-94	91.28	Callose synthase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G0...	[more]
A0A6J1HN98	2.2e-92	90.31	1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111466194 PE=3 SV=1	[more]
A0A6J1EP57	2.2e-92	90.31	1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111436119 PE=3 SV=1	[more]

BLAST of Lsi06G004600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011660099.1	2.2e-94	91.79	callose synthase 9 [Cucumis sativus] >KAE8653490.1 hypothetical protein Csa_0072...	[more]
XP_038878486.1	6.4e-94	91.33	callose synthase 9-like [Benincasa hispida] >XP_038878487.1 callose synthase 9-l...	[more]
KAA0042246.1	1.4e-93	91.28	callose synthase 9 [Cucumis melo var. makuwa]	[more]
XP_008451107.1	1.4e-93	91.28	PREDICTED: callose synthase 9 [Cucumis melo] >XP_016900973.1 PREDICTED: callose ...	[more]
XP_023531967.1	4.6e-92	90.31	callose synthase 9-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi06G004600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07160.1	2.8e-71	67.53	glucan synthase-like 10	[more]
AT2G36850.1	4.2e-51	49.22	glucan synthase-like 8	[more]
AT2G31960.1	1.2e-16	30.77	glucan synthase-like 3	[more]
AT2G31960.2	1.2e-16	30.77	glucan synthase-like 3	[more]
AT1G06490.1	1.5e-16	28.86	glucan synthase-like 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12741:SF81	BNAA01G32440D PROTEIN	coord: 4..196
None	No IPR available	PANTHER	PTHR12741	LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1	coord: 4..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G004600.1	Lsi06G004600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006075	(1->3)-beta-D-glucan biosynthetic process
biological_process	GO:0071555	cell wall organization
biological_process	GO:0008360	regulation of cell shape
cellular_component	GO:0000148	1,3-beta-D-glucan synthase complex
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003843	1,3-beta-D-glucan synthase activity