Homology
BLAST of Lsi06G004510 vs. ExPASy Swiss-Prot
Match:
P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)
HSP 1 Score: 973.0 bits (2514), Expect = 1.5e-282
Identity = 456/591 (77.16%), Postives = 513/591 (86.80%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+QNPPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
BLAST of Lsi06G004510 vs. ExPASy Swiss-Prot
Match:
P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)
HSP 1 Score: 955.7 bits (2469), Expect = 2.5e-277
Identity = 444/574 (77.35%), Postives = 494/574 (86.06%), Query Frame = 0
Query: 13 PFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIR 72
P +I F +I FGI S SQ RHYKWE+E MFW+PDC EN+VMGINGQFPGPTIR
Sbjct: 10 PNFLILFFFGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 69
Query: 73 ANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVVDK 132
AN GDT++VE+INKL TE VVIHWHGILQ+ TPWADGTASISQCA NPGET+ Y F VD
Sbjct: 70 ANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 129
Query: 133 AGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLS 192
GT+FYHGHLGMQR+AGLYGSLIVD +GKKEPF YD +INLLLSDWWH+S++KQE GLS
Sbjct: 130 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 189
Query: 193 SSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIR 252
S P+RWIGEP++IL+NGRGQ +CSIAAKY +E CKLKGSE CAP+I V P KTYRIR
Sbjct: 190 SKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIR 249
Query: 253 IASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYW 312
IASTT+L+ALNFAIGNH LL+VEADGNYVQPF TSDIDIYSGESYSVLITTDQ P+ENYW
Sbjct: 250 IASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYW 309
Query: 313 VSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSP 372
VS+G RGR P TPP LTLLNYLPNSVSKLP SPPPETP WDD+D SKNFTY+I A MGSP
Sbjct: 310 VSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSP 369
Query: 373 KPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPET 432
KPPVKSNRRIFLLNTQ N+ G+ KWAIN+VSLALPPTPYL A++ L FDQNPPPE
Sbjct: 370 KPPVKSNRRIFLLNTQ-NVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEV 429
Query: 433 FPEDYDIYTPPST-STRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWV 492
FPEDYDI TPP+ T+ GNG+Y+F++GEIVDVILQNAN ++++ SEIHPWHLHGHDFWV
Sbjct: 430 FPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWV 489
Query: 493 LGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHM 552
LGYGDGKF+++ +E LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHM
Sbjct: 490 LGYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 549
Query: 553 GMGVVFAEGVHKVKRIPTKALACGETAKVLINNP 586
GMGVVFAEGV KV RIPTKALACG TAK LINNP
Sbjct: 550 GMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579
BLAST of Lsi06G004510 vs. ExPASy Swiss-Prot
Match:
P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)
HSP 1 Score: 941.0 bits (2431), Expect = 6.3e-273
Identity = 431/551 (78.22%), Postives = 481/551 (87.30%), Query Frame = 0
Query: 36 SQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIH 95
SQ RHYKWE+E MFW+P+C EN+VMGINGQFPGPTIRAN GD+++VE+ NKL TE VVIH
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 96 WHGILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLI 155
WHGILQ+ TPWADGTASISQCA NPGET+ Y F VD GT+FYHGHLGMQR+AGLYGSLI
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 156 VDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNC 215
VD +GKKEPF YD +INLLLSDWWH+S++KQE GLSS P+RWIGEP++IL+NGRGQ +C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180
Query: 216 SIAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVE 275
SIAAKY +E CKLKGSE CAP+I V P KTYRIRIASTT+L+ALNFAIGNHQLL+VE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240
Query: 276 ADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLP 335
ADGNYVQPF TSDIDIYSGESYSVLITTDQ P+ENYWVS+G R R P TPP LTLLNYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300
Query: 336 NSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGF 395
NSVSKLP SPPP+TP WDD+D SKNFTY+I A MGSPKPPVK NRRIFLLNTQ N+ G+
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQ-NVINGY 360
Query: 396 TKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIYTPPST-STRTGNGIY 455
KWAIN+VSLALPPTPYL A++ L FDQNPPPE FPEDYDI TPP+ TR GNG+Y
Sbjct: 361 VKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVY 420
Query: 456 RFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPP 515
+F++GE+VDVILQNAN ++++ SE HPWHLHGHDFWVLGYGDGKFS++ +E LNLKNPP
Sbjct: 421 QFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPP 480
Query: 516 LRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALAC 575
LRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMGMGVVFAEGV KV RIPTKALAC
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540
Query: 576 GETAKVLINNP 586
G TAK LINNP
Sbjct: 541 GGTAKSLINNP 549
BLAST of Lsi06G004510 vs. ExPASy Swiss-Prot
Match:
Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)
HSP 1 Score: 830.1 bits (2143), Expect = 1.6e-239
Identity = 383/576 (66.49%), Postives = 464/576 (80.56%), Query Frame = 0
Query: 11 SMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPT 70
S+ FL FLL I+ + S ++TRH+KW++E + WSPD E++VMGINGQFPGPT
Sbjct: 3 SLGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPT 62
Query: 71 IRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVV 130
IRA GDT+ V + NKL TE VVIHWHGI Q TPWADGTA+ISQCA NPGET+LYRF V
Sbjct: 63 IRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKV 122
Query: 131 DKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAG 190
DKAGTYFYHGH GMQR+AGLYGSLIV++ EG+KEPF YD + NLLLSDWWHK ++QE
Sbjct: 123 DKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVD 182
Query: 191 LSSSPMRWIGEPESILINGRGQTNCSIAAKYKK-GVEQCKLKGSEECAPFILKVKPNKTY 250
LSS+P+RWIGEP+++L+NGRGQ NCS+AA++ K + QCKL+G E+ AP IL+V+PNK Y
Sbjct: 183 LSSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIY 242
Query: 251 RIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTE 310
R+R+ASTT+L +L+ AIG H++++VEADGNYVQPF D+DIYSGESYSVL TDQ PT+
Sbjct: 243 RLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTK 302
Query: 311 NYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATM 370
NYW+SI VRGR+PKTP LTLLNYLPNS SK P PPP P W+DY+HSK+F+ KI A M
Sbjct: 303 NYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALM 362
Query: 371 GSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPP 430
GSPKPP +++RRI LLNTQN + G+TKWAINNVSL LP YL +IR G+N FD PP
Sbjct: 363 GSPKPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPP 422
Query: 431 PETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHD 490
P+ FP+DYD+ P++++ GNG+Y + +D+ILQNAN L KD SEIHPWHLHGHD
Sbjct: 423 PDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHD 482
Query: 491 FWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPH 550
FWVLGYG+GKF S+ D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVW FHCHIEPH
Sbjct: 483 FWVLGYGEGKF-SEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPH 542
Query: 551 LHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINN 585
LHMGMGV+FAEGVH VK+IP +ALACG T K+L++N
Sbjct: 543 LHMGMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575
BLAST of Lsi06G004510 vs. ExPASy Swiss-Prot
Match:
M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)
HSP 1 Score: 770.8 bits (1989), Expect = 1.1e-221
Identity = 351/562 (62.46%), Postives = 432/562 (76.87%), Query Frame = 0
Query: 24 ISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEM 83
I I A + R Y WE+E F PDC E +VM +NGQFPGPTI A GDTI+V +
Sbjct: 6 IVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHL 65
Query: 84 INKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLG 143
NKL TE +VIHWHGI Q +PWADG A ++QCA +PGET+ Y F VDK GT+FYHGH G
Sbjct: 66 TNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYG 125
Query: 144 MQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPE 203
MQR+AGLYGSLI+D+ +GKKEP +YD + NLLLSDWWH+ V QE GLSS PMRWIGE +
Sbjct: 126 MQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQ 185
Query: 204 SILINGRGQTNCSIAAKYKK-GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSAL 263
SILINGRGQ NCS+AA++ + C K ++CAP L V+PNKTYRIR+AS+T+L++L
Sbjct: 186 SILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASL 245
Query: 264 NFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKP 323
NFA+ H+L++VEADGNY+ PF T DIDIYSGE+YSVL+TTDQ P++NY+++ GVRGRKP
Sbjct: 246 NFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKP 305
Query: 324 KTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNRRI 383
TPPALT+LNY+ S+LP SPPPETP+W+D+D SKNF+ KI A MGSP PP + R+
Sbjct: 306 NTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERL 365
Query: 384 FLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIYTP 443
LLNTQ N+ GFTKWAINNVSLA+P TPYL +++ L F+++ PP+ +P DYDI TP
Sbjct: 366 ILLNTQ-NLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTP 425
Query: 444 P-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSS 503
P + + + GN F VDVILQNAN L + SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 426 PRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKP 485
Query: 504 DVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGV 563
VDEK NLKNPPLRNTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAEG+
Sbjct: 486 GVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGL 545
Query: 564 HKVKRIPTKALACGETAKVLIN 584
+++ ++P +AL CG T + L+N
Sbjct: 546 NRIGKVPDEALGCGLTKQFLMN 566
BLAST of Lsi06G004510 vs. ExPASy TrEMBL
Match:
Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)
HSP 1 Score: 1054.3 bits (2725), Expect = 1.9e-304
Identity = 490/588 (83.33%), Postives = 535/588 (90.99%), Query Frame = 0
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K+ANQPLF MPF IF +FSI GFGITLS+A SQTRH+KWE+E MF SPDC ENLVMGI
Sbjct: 4 KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
N QFPGPTIRANVGDT++VE+INKL+TE VVIHWHGILQK TPWADGTASISQCA NPGE
Sbjct: 64 NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILK 242
SV KQE GLSSSPMRWIGEP+SILINGRGQ+NCSI AKY K +EQCKL GSEECAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LL+VEADGNYVQPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 362
TDQ P++NYWVS+GVRGR+PKTPP LTLLNYLPNS SKLPISPPPETP+W+DYD SKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 YKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLND 422
Y+ILA MGSPKPPVKS RRIFLLNTQN M+GG+TKWAINNVSL LP TPYL AI+IG+ND
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDQNPPPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHP 482
TFDQNPPPET+PE+YDI+ PP+ +T TGNG+YRF+LGEIVDV+LQNANTL SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYG+GKFS+ VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of Lsi06G004510 vs. ExPASy TrEMBL
Match:
A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)
HSP 1 Score: 1050.0 bits (2714), Expect = 3.6e-303
Identity = 490/588 (83.33%), Postives = 533/588 (90.65%), Query Frame = 0
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K ANQPLF MPF IF +FSI GFGITLS+A SQTRH+KWE+E MF SPDC ENLVMGI
Sbjct: 4 KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
N QFPGPTIRANVGDTI+VE+INKL+TE VVIHWHGILQK TPWADGTASISQCA NPGE
Sbjct: 64 NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILK 242
SV KQE GLSSSPMRWIGEP+SILINGRGQ+NCSI AKY K +EQCKL GSE CAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LL+VEADGNYVQPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 362
TDQ P++NYWVS+GVRGR+PKTPP LTLLNYLPNS SKLPISPPPETP+W+DYD SKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 YKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLND 422
Y+ILA MGSPKPPVKS RRIFLLNTQN M+GG+TKWAINNVSL LP TPYL AI+IG+ND
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDQNPPPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHP 482
TFDQNPPPET+PE+YDI+ PP+ +T TGNG+YRF+LGEIVDV+LQNANTL SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYG+GKFS+ VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of Lsi06G004510 vs. ExPASy TrEMBL
Match:
A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)
HSP 1 Score: 980.3 bits (2533), Expect = 3.5e-282
Identity = 460/591 (77.83%), Postives = 514/591 (86.97%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFLV F+L SII GFGITLSEA + RHYKWE+E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLVFFVL-SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGINGQFPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILINGRGQ +CSIAAKYK G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P+ENYWVSIGVR R PKTPP LT+LNYLPNSVSKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYTPP-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
L+ F+QNPPPETFP DYDI PP + T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKFS+ D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGV+FAEGVHKV IP A+ACG TA +I N +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587
BLAST of Lsi06G004510 vs. ExPASy TrEMBL
Match:
Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)
HSP 1 Score: 978.0 bits (2527), Expect = 1.7e-281
Identity = 459/591 (77.66%), Postives = 513/591 (86.80%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFLV F+L SII GFGITLSEA + RHYKWE+E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFLVFFVL-SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGINGQFPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILINGRGQ +CSIAAKYK G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V P K YRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P+ENYWVSIGVR R PKTPP LT+LNYLPNSVSKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYTPP-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
L+ F+QNPPPETFP DYDI PP + T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKFS+ D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGV+FAEGVHKV IP A+ACG TA +I N +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587
BLAST of Lsi06G004510 vs. ExPASy TrEMBL
Match:
A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)
HSP 1 Score: 973.0 bits (2514), Expect = 5.6e-280
Identity = 456/591 (77.16%), Postives = 513/591 (86.80%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++P F PFL FL+ SII GFGITLSEA + +HYKW++E MFWSPDC EN+V
Sbjct: 1 MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGING+FPGPTIRAN GD ++VE+ NKL TE VVIHWHGILQ+ TPWADGTASISQCA N
Sbjct: 61 MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EG+ EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS PMRWIGEP+SILING+GQ +CSIAAKY +G++QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V+P KTYRIRIASTT+L++LNFAIGNH+LL+VEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ P ENYWVSIGVR R PKTPP LTLLNYLPNS SKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPPV+ NRR+FLLNTQN ++ GF KWAINNVSLALPPTPYLAA+++
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420
Query: 421 LNDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN F+QNPPPETFP +YDI PP+ T TGNG+Y+F +GE VDVILQNAN L + SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKF + DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVH V IP KALACG TA L+ N + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587
BLAST of Lsi06G004510 vs. NCBI nr
Match:
XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])
HSP 1 Score: 1063.1 bits (2748), Expect = 8.4e-307
Identity = 493/590 (83.56%), Postives = 539/590 (91.36%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVM 60
MAK ANQPLF +PFLVIFL+FS+ISGFGITLSEAV+ TR++KWE+E MFWSPDC ENLVM
Sbjct: 1 MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60
Query: 61 GINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNP 120
GINGQFPGPTIRANVGDTIIVE++NKL TE VVIHWHGILQ+ETPWADGTASISQCA NP
Sbjct: 61 GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120
Query: 121 GETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWW 180
GET++Y+F DK GTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPF YDE+INLLLSDWW
Sbjct: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180
Query: 181 HKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFI 240
HKS +KQE GLSSSPMRWIGEPE+ILINGRGQ +CSIAAKY K +EQCKLKG E+CAPFI
Sbjct: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240
Query: 241 LKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVL 300
L+VKP KTYRIRIASTTSLSALN AIGNHQLL+VEADGNYV+PF+TSDIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300
Query: 301 ITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKN 360
ITT+Q P +NYWVS+GVRGR PKTPPALTLLNYLPNSV+KLP+SPPPETP W+DY SKN
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360
Query: 361 FTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGL 420
FTY+ILA MGSP PP+K NRRIFLLNTQN M+GGFTKWAINNVSL+LP TPYL AI+IGL
Sbjct: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420
Query: 421 NDTFDQNPPPETFPEDYDIYT-PPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEI 480
N+TF+QNPPPETF EDYDI+ PP+ +T T NG+Y F LGE VDVILQNANTL SEI
Sbjct: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480
Query: 481 HPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
HPWHLHGHDFWVLGYG+GKFS+ VDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
Query: 541 PFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
FHCHIEPHLHMGMGV+FAEGVHKV IPTKALACG TAKVLINNPT+KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 590
BLAST of Lsi06G004510 vs. NCBI nr
Match:
NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])
HSP 1 Score: 1054.3 bits (2725), Expect = 3.9e-304
Identity = 490/588 (83.33%), Postives = 535/588 (90.99%), Query Frame = 0
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K+ANQPLF MPF IF +FSI GFGITLS+A SQTRH+KWE+E MF SPDC ENLVMGI
Sbjct: 4 KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
N QFPGPTIRANVGDT++VE+INKL+TE VVIHWHGILQK TPWADGTASISQCA NPGE
Sbjct: 64 NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILK 242
SV KQE GLSSSPMRWIGEP+SILINGRGQ+NCSI AKY K +EQCKL GSEECAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LL+VEADGNYVQPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 362
TDQ P++NYWVS+GVRGR+PKTPP LTLLNYLPNS SKLPISPPPETP+W+DYD SKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 YKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLND 422
Y+ILA MGSPKPPVKS RRIFLLNTQN M+GG+TKWAINNVSL LP TPYL AI+IG+ND
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDQNPPPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHP 482
TFDQNPPPET+PE+YDI+ PP+ +T TGNG+YRF+LGEIVDV+LQNANTL SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYG+GKFS+ VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of Lsi06G004510 vs. NCBI nr
Match:
TYK09892.1 (L-ascorbate oxidase-like [Cucumis melo var. makuwa])
HSP 1 Score: 1050.0 bits (2714), Expect = 7.4e-303
Identity = 490/588 (83.33%), Postives = 533/588 (90.65%), Query Frame = 0
Query: 3 KLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGI 62
K ANQPLF MPF IF +FSI GFGITLS+A SQTRH+KWE+E MF SPDC ENLVMGI
Sbjct: 4 KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63
Query: 63 NGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGE 122
N QFPGPTIRANVGDTI+VE+INKL+TE VVIHWHGILQK TPWADGTASISQCA NPGE
Sbjct: 64 NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123
Query: 123 TYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHK 182
++ Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPF YDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183
Query: 183 SVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFILK 242
SV KQE GLSSSPMRWIGEP+SILINGRGQ+NCSI AKY K +EQCKL GSE CAPFILK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243
Query: 243 VKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
VKPNKTYRIRI STTSLSALNFAIGNH+LL+VEADGNYVQPFLTSDIDIYSGESYSVLI
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303
Query: 303 TDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFT 362
TDQ P++NYWVS+GVRGR+PKTPP LTLLNYLPNS SKLPISPPPETP+W+DYD SKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363
Query: 363 YKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLND 422
Y+ILA MGSPKPPVKS RRIFLLNTQN M+GG+TKWAINNVSL LP TPYL AI+IG+ND
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423
Query: 423 TFDQNPPPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHP 482
TFDQNPPPET+PE+YDI+ PP+ +T TGNG+YRF+LGEIVDV+LQNANTL SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483
Query: 483 WHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
WHLHGHDFWVLGYG+GKFS+ VDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543
Query: 543 HCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591
BLAST of Lsi06G004510 vs. NCBI nr
Match:
XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])
HSP 1 Score: 1005.0 bits (2597), Expect = 2.7e-289
Identity = 473/592 (79.90%), Postives = 525/592 (88.68%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEA-VSQTRHYKWEIENMFWSPDCTENLV 60
MAK+A++ LF PFL+ F+L SII GFGITLSEA + RHYKWE+E MFWSPDC EN+V
Sbjct: 1 MAKVADKSLFPKPFLIFFIL-SIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60
Query: 61 MGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATN 120
MGI+GQFPGPTIRANVGDT++VE+ NKL TE VVIHWHGI+Q+ TPWADGTASISQCA N
Sbjct: 61 MGIDGQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAIN 120
Query: 121 PGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDW 180
PGET+ YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EEGK EPF YDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDW 180
Query: 181 WHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPF 240
WH+SV+KQE GLSS P+RWIGEP++ILINGRGQ +CSIAAKY ++QC+LKG E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPF 240
Query: 241 ILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSV 300
IL V PNKTYRIRIASTT+L++LNFAIGNH+LL+VEADGNY+QPFLTSDIDIYSGESYSV
Sbjct: 241 ILHVLPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSV 300
Query: 301 LITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSK 360
LITTDQ +ENYWVSIGVR R PKTPP LT+LNYLPNSVSKLPISPPPETP W+D+D SK
Sbjct: 301 LITTDQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSK 360
Query: 361 NFTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIG 420
NFT++I A MGSPKPP++ NRRIFLLNTQN M+ GF KWAINNVSLALPPTPYLAA++
Sbjct: 361 NFTFRIFAAMGSPKPPMRYNRRIFLLNTQNRMN-GFVKWAINNVSLALPPTPYLAAMKFK 420
Query: 421 LNDTFDQNPPPETFPEDYDIYTPP-STSTRTGNGIYRFELGEIVDVILQNANTLEKDRSE 480
LN FDQNPPPETFP DYDI PP + +T TGNG+YRF +GEIVDVILQNAN L + SE
Sbjct: 421 LNTAFDQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSE 480
Query: 481 IHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
IHPWHLHGHDFWVLGYG+GKFS+ VDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLI-NNPTKKP 590
W FHCHIEPHLHMGMGVVFAEGVHKV RIPTKALACG AK LI NNP + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNPPQDP 590
BLAST of Lsi06G004510 vs. NCBI nr
Match:
XP_038880028.1 (L-ascorbate oxidase-like [Benincasa hispida])
HSP 1 Score: 991.1 bits (2561), Expect = 4.1e-285
Identity = 465/590 (78.81%), Postives = 515/590 (87.29%), Query Frame = 0
Query: 1 MAKLANQPLFSMPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVM 60
MA++AN+PLF MPF VIFL+ SIISGFGIT S+A SQ R++ WEI+ MFWSPDC EN++M
Sbjct: 1 MAEVANRPLFPMPFPVIFLVLSIISGFGITPSQAASQIRNHTWEIDYMFWSPDCVENIIM 60
Query: 61 GINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNP 120
GINGQFPGPTIRANV DTIIV + NKL+TE VVIHWHGILQ+ TPWADGTASISQCA NP
Sbjct: 61 GINGQFPGPTIRANVSDTIIVHVNNKLSTEGVVIHWHGILQRGTPWADGTASISQCAINP 120
Query: 121 GETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWW 180
GE + Y+FVVDKAGTYFYHGHLGMQR+AGLYGSLIVDLEEGKKEPF YDE+INLLLSDWW
Sbjct: 121 GENFTYQFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDLEEGKKEPFDYDEEINLLLSDWW 180
Query: 181 HKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQCKLKGSEECAPFI 240
HKSV+KQE GL+S+P RWIGEPESILINGRGQ NCSIAAKY +EQC+LK +EECAPFI
Sbjct: 181 HKSVHKQEVGLNSNPFRWIGEPESILINGRGQFNCSIAAKYNNNLEQCELKENEECAPFI 240
Query: 241 LKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVL 300
L+VKP KTYRIRIASTTSLSALN AIGNH LL+VEADGNYV PF+T DIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHSLLVVEADGNYVAPFVTYDIDIYSGESYSVL 300
Query: 301 ITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKN 360
ITT+Q P +NYWVS+GVRGR P+T PALTLLNYLPNSV KLPISPPPETP+WDD+ SK
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPETAPALTLLNYLPNSVDKLPISPPPETPKWDDFARSKR 360
Query: 361 FTYKILATMGSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGL 420
FT +ILAT PKPPV NRRIFLLNTQN M GG KWAINNVSL LP TPYL AI+IGL
Sbjct: 361 FTGRILATEDIPKPPVNFNRRIFLLNTQNLMKGGSIKWAINNVSLVLPSTPYLGAIKIGL 420
Query: 421 NDTFDQNPPPETFPEDYDI-YTPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEI 480
TFDQNPPPETFPEDYDI PP+ +T TGNG+Y+FELGE+VDVILQNAN L+++ S+I
Sbjct: 421 KTTFDQNPPPETFPEDYDINKPPPNPNTTTGNGVYKFELGEVVDVILQNANMLKENTSDI 480
Query: 481 HPWHLHGHDFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
HPWHLHGHDFWVLGYG+GKFS+DVDE LNL+NPPL+NTVVIFP GWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSADVDENNLNLENPPLKNTVVIFPRGWTAIRFVADNPGVW 540
Query: 541 PFHCHIEPHLHMGMGVVFAEGVHKVKRIPTKALACGETAKVLINNPTKKP 590
FHCHIEPHLHMGMGV+FAE VH+V IPT ALACG A+ LI P KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEEVHRVGTIPTHALACGGIARTLIYRPATKP 590
BLAST of Lsi06G004510 vs. TAIR 10
Match:
AT5G21105.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 772.7 bits (1994), Expect = 2.1e-223
Identity = 348/553 (62.93%), Postives = 429/553 (77.58%), Query Frame = 0
Query: 34 AVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVV 93
A + R Y WE+E +WSPDC E VM +NG+FPGPTI+A GDTI+V + NKLTTE +V
Sbjct: 33 ASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLV 92
Query: 94 IHWHGILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGS 153
IHWHGI Q +PWADG A ++QCA NPGET+ Y F V+K GT+FYHGH GMQR+AGLYGS
Sbjct: 93 IHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGS 152
Query: 154 LIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQT 213
LIVD+ +GK E +YD + NLLLSDWWH+++ QE GLSS PMRWIGE +SILINGRGQ
Sbjct: 153 LIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQF 212
Query: 214 NCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQL 273
NCS+AA++ + C K ++CAP IL V+PNKTYRIR++STT+L++LN A+ H+L
Sbjct: 213 NCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKL 272
Query: 274 LLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLL 333
++VEADGNY+ PF T DIDIYSGESYSVL+TTDQ P++NY++S+GVRGRKP T ALT+L
Sbjct: 273 VVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTIL 332
Query: 334 NYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNRRIFLLNTQNNM 393
NY+ SKLP SPPP TP+WDD++ SKNF+ KI + MGSP PP K +R+ LLNTQ N+
Sbjct: 333 NYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQ-NL 392
Query: 394 SGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIYTPPS-TSTRTG 453
G+TKWAINNVSL P TPYL +++ L F++ PP ++ DYDI PP +T TG
Sbjct: 393 IDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTG 452
Query: 454 NGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNL 513
NGIY F VDVI+QNAN L+ SEIHPWHLHGHDFWVLGYGDGKF +DEK NL
Sbjct: 453 NGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNL 512
Query: 514 KNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVKRIPTK 573
KNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAEG++++ ++P +
Sbjct: 513 KNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDE 572
Query: 574 ALACGETAKVLIN 584
AL CG T + L+N
Sbjct: 573 ALGCGLTKQFLMN 584
BLAST of Lsi06G004510 vs. TAIR 10
Match:
AT5G21100.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 729.9 bits (1883), Expect = 1.6e-210
Identity = 344/578 (59.52%), Postives = 435/578 (75.26%), Query Frame = 0
Query: 12 MPFLVIFLLFSIISGFGITLSEAVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTI 71
M +V +LL ++ F + V T WE+E +W PDC E +VM INGQFPGPTI
Sbjct: 1 MAVIVWWLLTVVVVAFHSASAAVVEST----WEVEYKYWWPDCKEGIVMAINGQFPGPTI 60
Query: 72 RANVGDTIIVEMINKLTTESVVIHWHGILQKETPWADGTASISQCATNPGETYLYRFVVD 131
A GDT+I+ ++NKL+TE VVIHWHGI QK TPWADG A ++QC NPGET+ Y+F+VD
Sbjct: 61 DAVAGDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVD 120
Query: 132 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGL 191
KAGT+FYHGH GMQR++GLYG LIV KE YD + NLLLSDWWH+S++ QE L
Sbjct: 121 KAGTHFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELAL 180
Query: 192 SSSPMRWIGEPESILINGRGQTNCSIAAKYKKGVEQ--CKLKGSEECAPFILKVKPNKTY 251
SS PMRWIGEP+S+LINGRGQ NCS AA + KG E+ C K +++CAP L+V+PN+ Y
Sbjct: 181 SSRPMRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVY 240
Query: 252 RIRIASTTSLSALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTE 311
R+RIASTT+L++LN A+ HQL++VEADGNYV PF +DID+YSGE+YSVL+ T+ P++
Sbjct: 241 RLRIASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSK 300
Query: 312 NYWVSIGVRGRKPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATM 371
YW+S+GVRGR+PKTP ALT++NY+ + S+ P PPP TP W+D D SK+F+ KI A
Sbjct: 301 KYWISVGVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAK 360
Query: 372 GSPKPPVKSNRRIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFD-QNP 431
G PKPP KS+ ++ LLNTQ N+ +TKW+INNVSL++P TPYL +IR GL +D ++P
Sbjct: 361 GYPKPPEKSHDQLILLNTQ-NLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSP 420
Query: 432 PPETFPEDYDIY-TPPSTSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGH 491
+ ++YDI PP+ +T G+GIY F G +VDVILQNAN L+ SEIHPWH+HGH
Sbjct: 421 AKKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGH 480
Query: 492 DFWVLGYGDGKFSSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEP 551
DFWVLGYG+GKF +DEK NLKNPPLRNTVV++P+GWTAIRFV DNPGVW FHCHIEP
Sbjct: 481 DFWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEP 540
Query: 552 HLHMGMGVVFAEGVHKVKR--IPTKALACGETAKVLIN 584
HLHMGMGVVF EGV ++ + IP +AL CG T K L+N
Sbjct: 541 HLHMGMGVVFVEGVDRIGKMEIPDEALGCGLTRKWLMN 569
BLAST of Lsi06G004510 vs. TAIR 10
Match:
AT5G21105.3 (Plant L-ascorbate oxidase )
HSP 1 Score: 702.6 bits (1812), Expect = 2.7e-202
Identity = 318/507 (62.72%), Postives = 390/507 (76.92%), Query Frame = 0
Query: 34 AVSQTRHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVV 93
A + R Y WE+E +WSPDC E VM +NG+FPGPTI+A GDTI+V + NKLTTE +V
Sbjct: 33 ASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLV 92
Query: 94 IHWHGILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGS 153
IHWHGI Q +PWADG A ++QCA NPGET+ Y F V+K GT+FYHGH GMQR+AGLYGS
Sbjct: 93 IHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGS 152
Query: 154 LIVDLEEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQT 213
LIVD+ +GK E +YD + NLLLSDWWH+++ QE GLSS PMRWIGE +SILINGRGQ
Sbjct: 153 LIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQF 212
Query: 214 NCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQL 273
NCS+AA++ + C K ++CAP IL V+PNKTYRIR++STT+L++LN A+ H+L
Sbjct: 213 NCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKL 272
Query: 274 LLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLL 333
++VEADGNY+ PF T DIDIYSGESYSVL+TTDQ P++NY++S+GVRGRKP T ALT+L
Sbjct: 273 VVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTIL 332
Query: 334 NYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNRRIFLLNTQNNM 393
NY+ SKLP SPPP TP+WDD++ SKNF+ KI + MGSP PP K +R+ LLNTQ N+
Sbjct: 333 NYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQ-NL 392
Query: 394 SGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIYTPPS-TSTRTG 453
G+TKWAINNVSL P TPYL +++ L F++ PP ++ DYDI PP +T TG
Sbjct: 393 IDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTG 452
Query: 454 NGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEKKLNL 513
NGIY F VDVI+QNAN L+ SEIHPWHLHGHDFWVLGYGDGKF +DEK NL
Sbjct: 453 NGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNL 512
Query: 514 KNPPLRNTVVIFPYGWTAIRFVADNPG 538
KNPPLRNT +++PYGWTAIRFV DNPG
Sbjct: 513 KNPPLRNTAILYPYGWTAIRFVTDNPG 538
BLAST of Lsi06G004510 vs. TAIR 10
Match:
AT4G39830.1 (Cupredoxin superfamily protein )
HSP 1 Score: 563.5 bits (1451), Expect = 2.0e-160
Identity = 271/553 (49.01%), Postives = 361/553 (65.28%), Query Frame = 0
Query: 39 RHYKWEIENMFWSPDCTENLVMGINGQFPGPTIRANVGDTIIVEMINKLTTESVVIHWHG 98
R +KWE++ F SPDC E LV+ ING+FPGPTI+A GDTI+VE+ N TE+V +HWHG
Sbjct: 36 RRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWHG 95
Query: 99 ILQKETPWADGTASISQCATNPGETYLYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDL 158
I Q TPW DG ++QC PGE ++Y+FVVD+ GTY YH H GMQR +GL G + V
Sbjct: 96 IRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVSP 155
Query: 159 EEGKKEPFQYDEDINLLLSDWWHKSVYKQEAGLSSSPMRWIGEPESILINGRGQTNCS-- 218
+ EPF YD D N LL+DW+HKS+ ++ GL+S P +W+GEP+S++I GRG+ NCS
Sbjct: 156 PATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCSNN 215
Query: 219 --IAAKYKKGVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLSALNFAIGNHQLLLV 278
GV C + + +C+ FIL V P KTYR+RI S T+LSAL+F I H L +V
Sbjct: 216 LTTPPSLVSGV--CNVSNA-DCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVV 275
Query: 279 EADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGRKPKTPPALTLLNYL 338
EADG+YV+PF ++ +YSGE+YSVL+ DQ P NYW++ + R TPPA +LNY
Sbjct: 276 EADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYY 335
Query: 339 PNSVSKLPISPPPET----PQWDDYDHSKNFTYKILATMGSPKP-PVKSNRRIFLLNTQN 398
PN + P P E+ P+W+D + I A G P S++ I LLNTQN
Sbjct: 336 PNHPRRRP--PTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQN 395
Query: 399 NMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFD-QNPPPETF-PEDYDIYTPP-STS 458
++ G+ +W++NNVS P TPYL A++ L + FD + PE + +YDI+ P + +
Sbjct: 396 EVN-GYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNAN 455
Query: 459 TRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKFSSDVDEK 518
T +GIYR VDVILQNANT+ + SE HPWHLHGHDFWVLGYG+GKF+ D K
Sbjct: 456 ATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPK 515
Query: 519 KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVKR 578
+ N +P +NTV + P+GWTA+RF ADNPGVW FHCHIE H MGMG+VF G+ KV
Sbjct: 516 RYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSS 575
Query: 579 IPTKALACGETAK 580
+P+ + CG+T +
Sbjct: 576 LPSSIMGCGQTKR 582
BLAST of Lsi06G004510 vs. TAIR 10
Match:
AT5G21105.2 (Plant L-ascorbate oxidase )
HSP 1 Score: 523.5 bits (1347), Expect = 2.2e-148
Identity = 240/384 (62.50%), Postives = 298/384 (77.60%), Query Frame = 0
Query: 203 ESILINGRGQTNCSIAAKYKK--GVEQCKLKGSEECAPFILKVKPNKTYRIRIASTTSLS 262
+SILINGRGQ NCS+AA++ + C K ++CAP IL V+PNKTYRIR++STT+L+
Sbjct: 11 KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70
Query: 263 ALNFAIGNHQLLLVEADGNYVQPFLTSDIDIYSGESYSVLITTDQTPTENYWVSIGVRGR 322
+LN A+ H+L++VEADGNY+ PF T DIDIYSGESYSVL+TTDQ P++NY++S+GVRGR
Sbjct: 71 SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130
Query: 323 KPKTPPALTLLNYLPNSVSKLPISPPPETPQWDDYDHSKNFTYKILATMGSPKPPVKSNR 382
KP T ALT+LNY+ SKLP SPPP TP+WDD++ SKNF+ KI + MGSP PP K +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190
Query: 383 RIFLLNTQNNMSGGFTKWAINNVSLALPPTPYLAAIRIGLNDTFDQNPPPETFPEDYDIY 442
R+ LLNTQ N+ G+TKWAINNVSL P TPYL +++ L F++ PP ++ DYDI
Sbjct: 191 RLILLNTQ-NLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 250
Query: 443 TPPS-TSTRTGNGIYRFELGEIVDVILQNANTLEKDRSEIHPWHLHGHDFWVLGYGDGKF 502
PP +T TGNGIY F VDVI+QNAN L+ SEIHPWHLHGHDFWVLGYGDGKF
Sbjct: 251 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 310
Query: 503 SSDVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAE 562
+DEK NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 311 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 370
Query: 563 GVHKVKRIPTKALACGETAKVLIN 584
G++++ ++P +AL CG T + L+N
Sbjct: 371 GLNRIGKVPDEALGCGLTKQFLMN 393
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P14133 | 1.5e-282 | 77.16 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1 | [more] |
| P24792 | 2.5e-277 | 77.35 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2 | [more] |
| P37064 | 6.3e-273 | 78.22 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1 | [more] |
| Q40588 | 1.6e-239 | 66.49 | L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1 | [more] |
| M4DUF2 | 1.1e-221 | 62.46 | L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9M5B5 | 1.9e-304 | 83.33 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1 | [more] |
| A0A5D3CF11 | 3.6e-303 | 83.33 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0... | [more] |
| A0A5A7UMY3 | 3.5e-282 | 77.83 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... | [more] |
| Q9M5B4 | 1.7e-281 | 77.66 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1 | [more] |
| A0A0A0LXB9 | 5.6e-280 | 77.16 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1 | [more] |
