Lsi06G004220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G004220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cysteine proteinase inhibitor
Location	chr06: 4638822 .. 4641947 (-)
RNA-Seq Expression	Lsi06G004220
Synteny	Lsi06G004220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATAGTATATTGCAAAGATAAAGCGAGCGTGTTCCTCACGCCCCATCATTCACGGAGGTCGCCATGACGATTCTGCAGACCAATCAATGCTTCTCGCTTCTTAAAATCTATAAAATGGAAGTGTTGGAATTCGATGGAAGCAGCAGATCAATCCATCGATCCACAATCAATCTCTCTCTCCCTCTGTATATTGAACTGAAGAGATCGTAATGGCGACTGTTGGAGGTATCAAGGAAGTGCATGGAAACGAGAACAGCCTCGAAATTGATGGTCTTGCTCGTTTTGCTGTTGATGAACACAACAAGAAAGAGGTTTGGTTTCCTTTTCCAATTCCCTTTCGCAAATTTTGAATTGGGTTCTGTTTTTTTTTATCCCTAAATTTGTGGATGTATCATTAGTTTGAGTTTTATTGTGTGGTTTCTGCGGTTATCTACCTTCATTTTTGTGTTCTTATGTATATTCGTGTTGATTGTGGATGATCATTTCTTCAATCGAGAAGGAGGTTTTCTGTATTACTTCTCAAACGAACAGGAACAATAGTGGGTTGGATAGGGATCGAGATTTAAGCCTTGTAATTTTAGTCTCAAAATTCCTTTTTGTATTAACTGTGTGGGCTTAAGAAGAAAAAGTTTTTGATGTCTTGTAGATCCAAACCTCTGGATTAGGCACCAATACGACAAGGCGGCTACATTGTAGTTAACAGTTTTGTATTGTTGGATGTAGGATAGAAAAACTGTCAAGGTATCTCGAATTTATAACCTTATCAAGGGGTTATTAAGGAAAACAAACAAACTTTAGAATACACATTCAATGGTTATTATGAGTCAGCCATATTGTGACAAGTCTTATCAAATAGTTTGTCAGCAGATACCGGGTAATTCGAATAGTCAGCCTCAGTCTCTGGTGTTATGGTCTAATGCAGTTAAACATTTCTCTCACAGTTTTGATAGTTTTGATTTTGATTACATCATGTTGTAAATTATCATTTCTTGTATTTTGAGCATTAAACTTTTTTCATTTCATCAGTGAAAGGTCTTGTTTCTTTTTCAAAGAAAAAAAAAAGATTTTCTGCGGATACCGGGCAATCGAAAAGTTCTGATTGAAAACATGTATGTGTGCAGAATGCTCTTCTGGAATTTAGCAAGGTTGTGAAGGTGAAGGAGCAGGTGGTCTCTGGCACACTTTATTACATAACTCTTGAGGCAAGTGAGGGCGGTCAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTCAAGCTGTGGGAGAATTTTAAGCAGCTTCAAGAGTTCAAGCCAGTTGGTGATGGGTCTGTGGGTTCCTCCGCTTAGTTTTCTAGGTAACCAAACTGCTTTGTTAATTTCTGCATTATCATGATACTCCAAAATCTAAATCTTAGGCATCTAGTACTATATTTTAATGTACATGGAATTATCTCCTTTTATCTTATGAAGAATTCTTAGTCAAATTTTAAAATCAAATACAAGTTTTGAAAAATTGCTTGTTAGCCTTCAAAATTTGACTTAGATTATTAGCTTGTTCCTGAAAGTAGATTGCAAAGCATAGAAACACGTAAGTGAAAATAGTGTTTATAAACATAATTTTAGAAACAAATAACAAAAACAAAAAGTTATCTAAGGAGACCAAATTAGAACCTGGATGGCCAATAACCGGGATGCAATTACTAATTCGGAAAAAGTTAATCGAAACTTAACGCAAAAACATTAAGTAGTTAAACATGAGCATAATTCAATAGTTAGGATACCATTTTTTTTTCTTAAGGTCGAAGAGTTGAATCTCCACCCTATATTAGGTAAACTAAAAGAGAAAAGAAGTAGCAAATAAAGAGTCAGTCTAGTTTAGACGGATTCAATTAATTCCTTAGCTAAGTATATAGTCTGTTTGAAATCTTCTGATGGTTATTAGAGGTTTGAGACTTTGCTGCTATTTTGGGAAGTTCAAACGGTTCAGGGGTGCTCTTTTCTTATTAAACTCTCTATCATCTTGATAGGTCTTGGTTCTAGGTCCTCCCATACCAACCACTACTTTTATCTGTATTCTCTTGATTCCTTTCATCACATTTAAGGATTTTTTTTAAAAGTACAATTTGGAGTAGTAGATTCGAACCACAAACCTTGTGGTTACTAATACACTCGTGTGCCAATTGAGCTATGCTCACTTTTGGCTACATTTAACGGTTTAGTCCACTGAGGAAGAAATTGATGTGAATTTCATAAATTTGCAGAGAAAGAAACACTAACAAGAAGTAGGAATATGATATATATACAAACAATCATGTATTAACAAAGTCACAAACAAAGGTAAAATTTGTAAAGTGAGCATAGCTCAATGGTAGTTATCATGTATTATTTTCTTCGAAGTTTCATAGTTTCATTTGTTGTACTAAAAAGTAACTATAAAGGTATAGTTGTGGATGATGGACCACTTTTACAGTTATAAATTAGCTCGCTTAATAAGTATTAGAGGCTCTGTATCTGAGTTTTTTTGTTTTAAATCTTTTGCTCTGCAAGCACCATATTTAATTAGTTCAAAAGTTTGGACAATTACTTGGATTTATGTGAACCCTCTAAAATCTTGTGTGTTTCTGAAATTTAGGGCCCTAAAATCATGATGATGCGTGGAATACGAATGGAGCAAAATCCTTTGCTTTATATGTAGTGTCTTTGATTTAATAAATTATGTAAAGCTATGGATTGTATGGATATTTATGATCAAATATCAATAGTTGTTGTGATTATGTGGCAATTGAATAAGATATGTATTTTCAGGAAGAAGTACCATATACTTAAACCTTAACTACAAAAGAGTAAAGCTACCGTTTTTCTTTTTGTTTTTTTTCCCCTTCATCTAAGTTCGACCATTGTCAAATGAGGTATCGTACCATAGAAATTTGTTGATAAAACTATGCTTATTATTGACAAAGGCCAAAAGCTAGCATAGTTGAAATAGCTCATTTGTTAAACTTTTGTTAACTGTCTCATACAAGCAACATTTTGATTCTGAAAATGCCTTTTAAAGCTTATTTGTTGAGGCAAGGTATAGATTTTAGATTCGGTCAGCTTTTAGTTCATCGAGCTGCCTTTAACGTCCTGTAATGATTAGAAAAAGGC

mRNA sequence

GAATAGTATATTGCAAAGATAAAGCGAGCGTGTTCCTCACGCCCCATCATTCACGGAGGTCGCCATGACGATTCTGCAGACCAATCAATGCTTCTCGCTTCTTAAAATCTATAAAATGGAAGTGTTGGAATTCGATGGAAGCAGCAGATCAATCCATCGATCCACAATCAATCTCTCTCTCCCTCTGTATATTGAACTGAAGAGATCGTAATGGCGACTGTTGGAGGTATCAAGGAAGTGCATGGAAACGAGAACAGCCTCGAAATTGATGGTCTTGCTCGTTTTGCTGTTGATGAACACAACAAGAAAGAGAATGCTCTTCTGGAATTTAGCAAGGTTGTGAAGGTGAAGGAGCAGGTGGTCTCTGGCACACTTTATTACATAACTCTTGAGGCAAGTGAGGGCGGTCAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTCAAGCTGTGGGAGAATTTTAAGCAGCTTCAAGAGTTCAAGCCAGTTGGTGATGGGTCTGTGGGTTCCTCCGCTTAGTTTTCTAGGGCCCTAAAATCATGATGATGCGTGGAATACGAATGGAGCAAAATCCTTTGCTTTATATGTAGTGTCTTTGATTTAATAAATTATGTAAAGCTATGGATTGTATGGATATTTATGATCAAATATCAATAGTTGTTGTGATTATGTGGCAATTGAATAAGATATGTATTTTCAGGAAGAAGTACCATATACTTAAACCTTAACTACAAAAGAGTAAAGCTACCGTTTTTCTTTTTGTTTTTTTTCCCCTTCATCTAAGTTCGACCATTGTCAAATGAGGTATCGTACCATAGAAATTTGTTGATAAAACTATGCTTATTATTGACAAAGGCCAAAAGCTAGCATAGTTGAAATAGCTCATTTGTTAAACTTTTGTTAACTGTCTCATACAAGCAACATTTTGATTCTGAAAATGCCTTTTAAAGCTTATTTGTTGAGGCAAGGTATAGATTTTAGATTCGGTCAGCTTTTAGTTCATCGAGCTGCCTTTAACGTCCTGTAATGATTAGAAAAAGGC

Coding sequence (CDS)

ATGGCGACTGTTGGAGGTATCAAGGAAGTGCATGGAAACGAGAACAGCCTCGAAATTGATGGTCTTGCTCGTTTTGCTGTTGATGAACACAACAAGAAAGAGAATGCTCTTCTGGAATTTAGCAAGGTTGTGAAGGTGAAGGAGCAGGTGGTCTCTGGCACACTTTATTACATAACTCTTGAGGCAAGTGAGGGCGGTCAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTCAAGCTGTGGGAGAATTTTAAGCAGCTTCAAGAGTTCAAGCCAGTTGGTGATGGGTCTGTGGGTTCCTCCGCTTAG

Protein sequence

MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA

Homology

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BLAST of Lsi06G004220 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 1.5e-33
Identity = 70/94 (74.47%), Postives = 81/94 (86.17%), Query Frame = 0


Query: 1  MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
          MA +GG ++V GN+NSLEID LARFAV+EHNKK+NALLEF +VV  ++QVVSGTLY ITL
Sbjct: 1  MAALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITL 60

Query: 61 EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGD 95
          EA +GGQKKVYEAKVW K W NFK+LQEFK VGD
Sbjct: 61 EAKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGD 94

[Close]

BLAST of Lsi06G004220 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-32
Identity = 68/101 (67.33%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA VGG+ +V  N+NS E++ LARFAVDEHNKKENALLEF++VVK KEQVV+GTL+++TL
Sbjct: 34  MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 93

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E  E GQKK+YEAKVWVK W NFK+LQEFKP  D    +S+
Sbjct: 94  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

[Close]

BLAST of Lsi06G004220 vs. ExPASy Swiss-Prot
Match: Q10992 (Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.3e-29
Identity = 63/77 (81.82%), Postives = 68/77 (88.31%), Query Frame = 0


Query: 16 SLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEASEGGQKKVYEAKV 75
          SLEID LARFAVDEHNKK+NALLEF KVV  KEQVV+G +YYITLEA+ GG KK YEAKV
Sbjct: 1  SLEIDELARFAVDEHNKKQNALLEFGKVVNTKEQVVAGKMYYITLEATNGGVKKTYEAKV 60

Query: 76 WVKLWENFKQLQEFKPV 93
          WVK WENFK+LQEFKPV
Sbjct: 61 WVKPWENFKELQEFKPV 77

[Close]

BLAST of Lsi06G004220 vs. ExPASy Swiss-Prot
Match: P31726 (Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 3.9e-29
Identity = 61/92 (66.30%), Postives = 75/92 (81.52%), Query Frame = 0


Query: 5   GGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEASE 64
           GGIK+V  NEN L++  LARFAV+EHN+K NALL F K+VK K QVV+GT+YY+T+E  +
Sbjct: 42  GGIKDVPANENDLQLQELARFAVNEHNQKANALLGFEKLVKAKTQVVAGTMYYLTIEVKD 101

Query: 65  GGQKKVYEAKVWVKLWENFKQLQEFKPVGDGS 97
           G  KK+YEAKVW K WENFKQLQEFKPV +G+
Sbjct: 102 GEVKKLYEAKVWEKPWENFKQLQEFKPVEEGA 133

[Close]

BLAST of Lsi06G004220 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 4.3e-28
Identity = 61/98 (62.24%), Postives = 78/98 (79.59%), Query Frame = 0


Query: 4   VGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEAS 63
           +GG  +     NS+E D LARFAVDEHNK+ENALLEF +VV+ KEQVV+GTL+++TLEA 
Sbjct: 48  LGGAHDAPSAANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEAL 107

Query: 64  EGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E G+KKVYEAKVWVK W +FK+LQEF+  GD +  ++A
Sbjct: 108 EAGRKKVYEAKVWVKPWLDFKELQEFRNTGDATTFTNA 145

[Close]

BLAST of Lsi06G004220 vs. ExPASy TrEMBL
Match: A0A6J1EYF3 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439577 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.1e-40
Identity = 86/101 (85.15%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA +GGIKEV  N+NS+E D LARFAV+EHNKKENALLEFSKVVKVKEQVVSGT+Y+ITL
Sbjct: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EAS+GGQKKVYEAK+WVK+WENFKQLQEFK VGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. ExPASy TrEMBL
Match: G0ZS05 (Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=LOC111467795 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.5e-39
Identity = 85/101 (84.16%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA +GGIKEV  N+NS+E D LARFAVDEHNKK NALLEFSKVVKVKEQVVSG +Y+ITL
Sbjct: 1   MALLGGIKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGAMYHITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EAS+GGQKKVYEAK+WVK+WENFKQLQEFK VGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. ExPASy TrEMBL
Match: A0A6J1D1L9 (Cysteine proteinase inhibitor OS=Momordica charantia OX=3673 GN=LOC111016451 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.1e-38
Identity = 82/101 (81.19%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA VGGIKEV GN+NS+EID LARFAV EHNKKENALLEF KVV VKEQVV+G +Y+ITL
Sbjct: 1   MANVGGIKEVSGNQNSVEIDDLARFAVAEHNKKENALLEFGKVVNVKEQVVAGAIYFITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EA++GG+KKVYEAK+WVK WENFKQLQEFK +GDGSVGSSA
Sbjct: 61  EATDGGKKKVYEAKIWVKAWENFKQLQEFKLLGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. ExPASy TrEMBL
Match: A0A5B7BRY3 (Cysteine proteinase inhibitor OS=Davidia involucrata OX=16924 GN=Din_040297 PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.0e-36
Identity = 76/101 (75.25%), Postives = 91/101 (90.10%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MAT+GGI+EV G++NS+E DGLARFAVDEHNKKENALLEF KVVKVK+QVV+GT+YYIT+
Sbjct: 1   MATLGGIREVEGSQNSVETDGLARFAVDEHNKKENALLEFKKVVKVKQQVVAGTMYYITV 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E ++GG KKVYEAKVWVK W NFK+LQEFKP+GD +  +SA
Sbjct: 61  EVTDGGVKKVYEAKVWVKPWMNFKELQEFKPIGDAASSASA 101

[Close]

BLAST of Lsi06G004220 vs. ExPASy TrEMBL
Match: A0A5A7UKU2 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00640 PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.0e-36
Identity = 82/101 (81.19%), Postives = 92/101 (91.09%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MATVGGIK+V  NENS+E+D LARFAV EHNKKEN+LLEFSKVVKVK+QVV+GT+YYIT+
Sbjct: 1   MATVGGIKDVPSNENSIEMDELARFAVAEHNKKENSLLEFSKVVKVKQQVVAGTVYYITV 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E +EGGQKKVYEAK+WVK WENFKQLQEFK V D SVGSSA
Sbjct: 61  EVTEGGQKKVYEAKIWVKHWENFKQLQEFKLV-DASVGSSA 100

[Close]

BLAST of Lsi06G004220 vs. NCBI nr
Match: XP_038879548.1 (cysteine proteinase inhibitor A-like [Benincasa hispida])

HSP 1 Score: 185.7 bits (470), Expect = 2.0e-43
Identity = 94/101 (93.07%), Postives = 97/101 (96.04%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MATVGGIKEV GNENS+EID LARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLY+ITL
Sbjct: 1   MATVGGIKEVPGNENSIEIDDLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYHITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EASEGGQKKVYEAK+WVK WENFKQLQEFK VGDGSVGSSA
Sbjct: 61  EASEGGQKKVYEAKIWVKQWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. NCBI nr
Match: XP_004144108.1 (cysteine proteinase inhibitor A [Cucumis sativus] >KAE8653481.1 hypothetical protein Csa_007376 [Cucumis sativus])

HSP 1 Score: 174.5 bits (441), Expect = 4.7e-40
Identity = 85/101 (84.16%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MATVGGIKEV  NENS+E+D LARFAVDEHNKKEN+LLEFSKVVKVKEQVV+GT+YYIT+
Sbjct: 1   MATVGGIKEVPSNENSIEVDELARFAVDEHNKKENSLLEFSKVVKVKEQVVAGTVYYITV 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E +EGGQKKVYEAK+WVK W+NFKQLQEFK  GDGSVGSSA
Sbjct: 61  EVTEGGQKKVYEAKIWVKQWQNFKQLQEFKLAGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. NCBI nr
Match: XP_022932964.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata])

HSP 1 Score: 173.3 bits (438), Expect = 1.0e-39
Identity = 86/101 (85.15%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA +GGIKEV  N+NS+E D LARFAV+EHNKKENALLEFSKVVKVKEQVVSGT+Y+ITL
Sbjct: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EAS+GGQKKVYEAK+WVK+WENFKQLQEFK VGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. NCBI nr
Match: KAG7022036.1 (hypothetical protein SDJN02_15765 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 172.6 bits (436), Expect = 1.8e-39
Identity = 86/101 (85.15%), Postives = 94/101 (93.07%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA +GGIKEV  N+NS+E D LARFAV+EHNKKENALLEFSKVVKVKEQVVSGT+Y+ITL
Sbjct: 1   MALLGGIKEVPANQNSVEADELARFAVNEHNKKENALLEFSKVVKVKEQVVSGTMYHITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EAS GGQKKVYEAK+WVK+WENFKQLQEFK VGDGSVGSSA
Sbjct: 61  EASNGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. NCBI nr
Match: XP_022968636.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >AEK84226.1 cysteine protease inhibitor, partial [Cucurbita maxima])

HSP 1 Score: 171.0 bits (432), Expect = 5.2e-39
Identity = 85/101 (84.16%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA +GGIKEV  N+NS+E D LARFAVDEHNKK NALLEFSKVVKVKEQVVSG +Y+ITL
Sbjct: 1   MALLGGIKEVPANQNSVETDELARFAVDEHNKKANALLEFSKVVKVKEQVVSGAMYHITL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           EAS+GGQKKVYEAK+WVK+WENFKQLQEFK VGDGSVGSSA
Sbjct: 61  EASDGGQKKVYEAKIWVKVWENFKQLQEFKLVGDGSVGSSA 101

[Close]

BLAST of Lsi06G004220 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 140.2 bits (352), Expect = 9.1e-34
Identity = 68/101 (67.33%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA VGG+ +V  N+NS E++ LARFAVDEHNKKENALLEF++VVK KEQVV+GTL+++TL
Sbjct: 34  MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 93

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E  E GQKK+YEAKVWVK W NFK+LQEFKP  D    +S+
Sbjct: 94  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 134

[Close]

BLAST of Lsi06G004220 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 140.2 bits (352), Expect = 9.1e-34
Identity = 68/101 (67.33%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 1   MATVGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITL 60
           MA VGG+ +V  N+NS E++ LARFAVDEHNKKENALLEF++VVK KEQVV+GTL+++TL
Sbjct: 1   MALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTL 60

Query: 61  EASEGGQKKVYEAKVWVKLWENFKQLQEFKPVGDGSVGSSA 102
           E  E GQKK+YEAKVWVK W NFK+LQEFKP  D    +S+
Sbjct: 61  EILEAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSS 101

[Close]

BLAST of Lsi06G004220 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 122.9 bits (307), Expect = 1.5e-28
Identity = 56/87 (64.37%), Postives = 73/87 (83.91%), Query Frame = 0


Query: 4   VGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEAS 63
           +GG+ ++ GN+NS EI+ LARFA+ EHNK++N +LEF K+VK +EQVV+GT+Y++TLEA 
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLEAK 94

Query: 64  EGGQKKVYEAKVWVKLWENFKQLQEFK 91
           EG Q K +EAKVWVK W NFKQLQEFK
Sbjct: 95  EGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Lsi06G004220 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 104.8 bits (260), Expect = 4.3e-23
Identity = 52/90 (57.78%), Postives = 67/90 (74.44%), Query Frame = 0


Query: 4   VGGIKEVHGNEN-SLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEA 63
           +GG  +   + N   EID +A FAV EHN++ENA+LE ++V+K  EQVV+G LY +TLE 
Sbjct: 45  LGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEV 104

Query: 64  SEGGQKKVYEAKVWVKLWENFKQLQEFKPV 93
            E G+KK+YEAKVWVK W NFKQLQEFK +
Sbjct: 105 IEAGEKKIYEAKVWVKPWMNFKQLQEFKNI 134

[Close]

BLAST of Lsi06G004220 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-22
Identity = 46/91 (50.55%), Postives = 67/91 (73.63%), Query Frame = 0


Query: 4   VGGIKEVHGNENSLEIDGLARFAVDEHNKKENALLEFSKVVKVKEQVVSGTLYYITLEAS 63
           VGG++++  N N L+++ LARFAVDEHNK EN  LE+ +++  K QVV+GT++++T+E +
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 64  EGGQKKVYEAKVWVKLWENFKQLQEFKPVGD 95
           +G   KVYEAKV  K WEN KQL+ F  + D
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESFNHLHD 100

The following BLAST results are available for this feature:

BLAST of Lsi06G004220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06445	1.5e-33	74.47	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q8H0X6	1.3e-32	67.33	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q10992	2.3e-29	81.82	Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]
P31726	3.9e-29	66.30	Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1	[more]
Q0JNR2	4.3e-28	62.24	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]

BLAST of Lsi06G004220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EYF3	5.1e-40	85.15	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439577 PE=3...	[more]
G0ZS05	2.5e-39	84.16	Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1D1L9	2.1e-38	81.19	Cysteine proteinase inhibitor OS=Momordica charantia OX=3673 GN=LOC111016451 PE=...	[more]
A0A5B7BRY3	2.0e-36	75.25	Cysteine proteinase inhibitor OS=Davidia involucrata OX=16924 GN=Din_040297 PE=3...	[more]
A0A5A7UKU2	2.0e-36	81.19	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Lsi06G004220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879548.1	2.0e-43	93.07	cysteine proteinase inhibitor A-like [Benincasa hispida]	[more]
XP_004144108.1	4.7e-40	84.16	cysteine proteinase inhibitor A [Cucumis sativus] >KAE8653481.1 hypothetical pro...	[more]
XP_022932964.1	1.0e-39	85.15	cysteine proteinase inhibitor A-like [Cucurbita moschata]	[more]
KAG7022036.1	1.8e-39	85.15	hypothetical protein SDJN02_15765 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022968636.1	5.2e-39	84.16	cysteine proteinase inhibitor A-like [Cucurbita maxima] >AEK84226.1 cysteine pro...	[more]

BLAST of Lsi06G004220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12490.2	9.1e-34	67.33	cystatin B	[more]
AT3G12490.1	9.1e-34	67.33	cystatin B	[more]
AT2G40880.1	1.5e-28	64.37	cystatin A	[more]
AT5G05110.1	4.3e-23	57.78	Cystatin/monellin family protein	[more]
AT5G12140.1	1.2e-22	50.55	cystatin-1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 2..92 e-value: 2.9E-34 score: 129.8
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 17..92 e-value: 1.0E-22 score: 80.2
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 5..91 e-value: 8.65916E-16 score: 64.8028
None	No IPR available	GENE3D	3.10.450.10		coord: 1..99 e-value: 7.5E-43 score: 147.1
None	No IPR available	PANTHER	PTHR11413:SF110	CYSTEINE PROTEINASE INHIBITOR 7	coord: 3..93
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 5..94
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 3..93
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 48..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G004220.1	Lsi06G004220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070417	cellular response to cold
biological_process	GO:0034605	cellular response to heat
biological_process	GO:0042631	cellular response to water deprivation
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity