Lsi06G003250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G003250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr06: 3655128 .. 3655340 (-)
RNA-Seq Expression	Lsi06G003250
Synteny	Lsi06G003250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTAATAAAGCTCTTGTAGAAGAGTGGTTCAATACCTACGTTAAACCCTTCATTGATGATTTCAAAATCAATTACATTATTGTTGGTGATAAGGCCATCCCAGGACTTGACAATTGCATATTGCCAGTCATGATGTCCCTTCAAGATCTTCTCAATGCACATTATCTTGGACAAGTCAAGCTCACCACGTTGATCGGGTATGGTTAA

mRNA sequence

ATGGCCACTAATAAAGCTCTTGTAGAAGAGTGGTTCAATACCTACGTTAAACCCTTCATTGATGATTTCAAAATCAATTACATTATTGTTGGTGATAAGGCCATCCCAGGACTTGACAATTGCATATTGCCAGTCATGATGTCCCTTCAAGATCTTCTCAATGCACATTATCTTGGACAAGTCAAGCTCACCACGTTGATCGGGTATGGTTAA

Coding sequence (CDS)

ATGGCCACTAATAAAGCTCTTGTAGAAGAGTGGTTCAATACCTACGTTAAACCCTTCATTGATGATTTCAAAATCAATTACATTATTGTTGGTGATAAGGCCATCCCAGGACTTGACAATTGCATATTGCCAGTCATGATGTCCCTTCAAGATCTTCTCAATGCACATTATCTTGGACAAGTCAAGCTCACCACGTTGATCGGGTATGGTTAA

Protein sequence

MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQVKLTTLIGYG

Homology

[Close]

BLAST of Lsi06G003250 vs. ExPASy TrEMBL
Match: A0A5A7UKS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001680 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-28
Identity = 62/69 (89.86%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNKALVEEWFNTY+KPF+DDFKINYIIVGDKA+PGLDN ILPVMMSLQDLLNA+Y GQ
Sbjct: 79  MATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQDLLNANYFGQ 138

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 139 VKLTTLVGY 147

[Close]

BLAST of Lsi06G003250 vs. ExPASy TrEMBL
Match: A0A1S3BQX5 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-28
Identity = 62/69 (89.86%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNKALVEEWFNTY+KPF+DDFKINYIIVGDKA+PGLDN ILPVMMSLQDLLNA+Y GQ
Sbjct: 90  MATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQDLLNANYFGQ 149

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 150 VKLTTLVGY 158

[Close]

BLAST of Lsi06G003250 vs. ExPASy TrEMBL
Match: A0A0A0LZ10 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-24
Identity = 56/69 (81.16%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           +A NK LVEEWFNTYVK F+DDF+INYIIVGDKAIP   N ILP+MMSLQDLLNA+YLGQ
Sbjct: 89  LANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNANYLGQ 148

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 149 VKLTTLVGY 157

[Close]

BLAST of Lsi06G003250 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 7.9e-24
Identity = 53/69 (76.81%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNK LVEEWFNTYV+P+++D+ INYI+VGDKAIPGLD+ ILPVM SLQDLLNAHY G+
Sbjct: 87  MATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKAIPGLDDNILPVMKSLQDLLNAHYFGE 146

Query: 61  VKLTTLIGY 70
            KLTTL+G+
Sbjct: 147 AKLTTLVGF 155

[Close]

BLAST of Lsi06G003250 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.9e-22
Identity = 53/69 (76.81%), Postives = 61/69 (88.41%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           M  N++ V EWFNTYV+P+I DF INYIIVG+KAIPGLD+CILPVM SLQ+LLNA YLGQ
Sbjct: 86  MKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDDCILPVMKSLQNLLNARYLGQ 145

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 146 VKLTTLVGY 154

[Close]

BLAST of Lsi06G003250 vs. NCBI nr
Match: KAA0055784.1 (putative glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo var. makuwa] >TYK10035.1 putative glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo var. makuwa])

HSP 1 Score: 135.2 bits (339), Expect = 2.2e-28
Identity = 62/69 (89.86%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNKALVEEWFNTY+KPF+DDFKINYIIVGDKA+PGLDN ILPVMMSLQDLLNA+Y GQ
Sbjct: 79  MATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQDLLNANYFGQ 138

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 139 VKLTTLVGY 147

[Close]

BLAST of Lsi06G003250 vs. NCBI nr
Match: XP_008451156.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo])

HSP 1 Score: 135.2 bits (339), Expect = 2.2e-28
Identity = 62/69 (89.86%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNKALVEEWFNTY+KPF+DDFKINYIIVGDKA+PGLDN ILPVMMSLQDLLNA+Y GQ
Sbjct: 90  MATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQDLLNANYFGQ 149

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 150 VKLTTLVGY 158

[Close]

BLAST of Lsi06G003250 vs. NCBI nr
Match: XP_038880473.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 131.7 bits (330), Expect = 2.4e-27
Identity = 59/69 (85.51%), Postives = 67/69 (97.10%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNKALVEEWFNTY+KPF+++FKINY+IVGDKAIPGLD CILPVMMS QDLLNA+Y+GQ
Sbjct: 89  MATNKALVEEWFNTYIKPFVNEFKINYVIVGDKAIPGLDICILPVMMSFQDLLNANYIGQ 148

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 149 VKLTTLVGY 157

[Close]

BLAST of Lsi06G003250 vs. NCBI nr
Match: KAG7011054.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 120.2 bits (300), Expect = 7.3e-24
Identity = 54/69 (78.26%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           MATNK LVE WFNTY+ P++DD+ INYI+VGDKAIPGLD+ ILPVM SLQDLLNAHYLG+
Sbjct: 87  MATNKTLVEVWFNTYIVPYVDDYTINYIVVGDKAIPGLDDNILPVMKSLQDLLNAHYLGR 146

Query: 61  VKLTTLIGY 70
           VKLTTL+G+
Sbjct: 147 VKLTTLVGF 155

[Close]

BLAST of Lsi06G003250 vs. NCBI nr
Match: KAE8653445.1 (hypothetical protein Csa_006879 [Cucumis sativus])

HSP 1 Score: 120.2 bits (300), Expect = 7.3e-24
Identity = 56/69 (81.16%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 1   MATNKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCILPVMMSLQDLLNAHYLGQ 60
           +A NK LVEEWFNTYVK F+DDF+INYIIVGDKAIP   N ILP+MMSLQDLLNA+YLGQ
Sbjct: 427 LANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNANYLGQ 486

Query: 61  VKLTTLIGY 70
           VKLTTL+GY
Sbjct: 487 VKLTTLVGY 495

[Close]

BLAST of Lsi06G003250 vs. TAIR 10
Match: AT2G39640.1 (glycosyl hydrolase family 17 protein )

HSP 1 Score: 40.8 bits (94), Expect = 5.2e-04
Identity = 22/66 (33.33%), Postives = 32/66 (48.48%), Query Frame = 0


Query: 4   NKALVEEWFNTYVKPFIDDFKINYIIVGDKAIPGLDNCIL----PVMMSLQDLLNAHYLG 63
           N A   +W    V PF    K  Y+ VG++ +   DN ++    P M SL + L A  L 
Sbjct: 93  NVANARQWVAANVLPFQQQIKFKYVCVGNEILASNDNNLISNLVPAMQSLNEALKASNLT 152

Query: 64  QVKLTT 66
            +K+TT
Sbjct: 153 YIKVTT 158

The following BLAST results are available for this feature:

BLAST of Lsi06G003250 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi06G003250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UKS6	1.1e-28	89.86	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BQX5	1.1e-28	89.86	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV...	[more]
A0A0A0LZ10	3.5e-24	81.16	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3...	[more]
A0A6J1HVR6	7.9e-24	76.81	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]
A0A6J1C4V7	1.9e-22	76.81	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ...	[more]

BLAST of Lsi06G003250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055784.1	2.2e-28	89.86	putative glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo var. makuwa] >TYK100...	[more]
XP_008451156.1	2.2e-28	89.86	PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo]	[more]
XP_038880473.1	2.4e-27	85.51	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
KAG7011054.1	7.3e-24	78.26	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]
KAE8653445.1	7.3e-24	81.16	hypothetical protein Csa_006879 [Cucumis sativus]	[more]

BLAST of Lsi06G003250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39640.1	5.2e-04	33.33	glycosyl hydrolase family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..70 e-value: 1.0E-12 score: 49.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G003250.1	Lsi06G003250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity