Lsi06G002980 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G002980 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.1e-37
Identity = 112/362 (30.94%), Postives = 174/362 (48.07%), Query Frame = 0

Query: 119 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 178
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 179 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESK 238
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 239 LTKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 298
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 299 QGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMG-LLND------------VFIV 358
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 359 GNSGYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEV-K 418
           G+SG+PL+ WLL PY  +  +  Q  FN++  E   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 419 LQELPVVLGACCVLHN-ICEMRKEKFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAH 454
              LP ++  CC+LHN I +M  +  D +   + +D      S  L    +   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of Lsi06G002980 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-34
Identity = 95/314 (30.25%), Postives = 158/314 (50.32%), Query Frame = 0

Query: 122 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 181
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 182 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDES 241
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 242 KLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 301
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 302 VDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLND-------------VFIVGNS 361
            D    F ++  GWPG M   ++L+ S  F+      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 362 GYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQK-RTEVKLQE 410
            YPL+ WL+ P+   + + +  AFNE+  ++++ A TAF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Lsi06G002980 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.1e-17
Identity = 69/261 (26.44%), Postives = 119/261 (45.59%), Query Frame = 0

Query: 154 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 213
           EL    + + T    AI    +V   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 214 IRKVLMPKFLQWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 273
           + +    +F+++P DE+ +  +K EF  ++G+P V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNR- 170

Query: 274 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFI 333
                 K  +S+    V D  G    V   WPGS+ D  VL++S L  +   G+  D ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWL 230

Query: 334 VGNSGYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVK 393
           +G+S + L  WL+ P  +   T  ++ +N       +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 394 LQELPV----VLGACCVLHNI 410
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Lsi06G002980 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 68/261 (26.05%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 154 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 213
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 214 IRKVLMPKFLQWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 273
           + +    +F+ +P DE+ +  +K EF  ++GIP V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNR- 170

Query: 274 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFI 333
                 K  +S+    V D  G    V   WPGS+ D  VL++S L  +   G+  + ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWL 230

Query: 334 VGNSGYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVK 393
           +G+S + L  WL+ P  +   T  ++ +N       +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 394 LQELPV----VLGACCVLHNI 410
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Lsi06G002980 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 68/261 (26.05%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 154 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 213
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 214 IRKVLMPKFLQWP-DESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 273
           + +    +F+ +P DE+ +  +K EF  ++G+P V G++   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR- 170

Query: 274 HTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFI 333
                 K  +S+    V D  G    V   WPGS+ D  VL++S L  +   G+  D ++
Sbjct: 171 ------KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWL 230

Query: 334 VGNSGYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVK 393
           +G+S + L  WLL P  +   T  ++ +N       +  +     L  R+ CL   ++  
Sbjct: 231 LGDSSFFLHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLD-GSKGA 290

Query: 394 LQELPV----VLGACCVLHNI 410
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Lsi06G002980 vs. ExPASy TrEMBL
Match: A0A5D3CDK0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001910 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.9e-231
Identity = 400/469 (85.29%), Postives = 426/469 (90.83%), Query Frame = 0

Query: 1   MDHNNT--FSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQE 60
           MD+N T  F+VN +P+KRRR D N  D   FNN SF DEN      + LLKLPCWF+ Q 
Sbjct: 1   MDNNPTTPFNVNPTPKKRRRSDPNSDD---FNNFSFTDENDEPT-DDPLLKLPCWFDPQP 60

Query: 61  ETQENWIMDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLW 120
           E+ ++W+MDS+K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLW
Sbjct: 61  ESPQSWLMDSQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNNPAGGGGTQQRRLW 120

Query: 121 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 180
           VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR
Sbjct: 121 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 180

Query: 181 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 240
           VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK
Sbjct: 181 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 240

Query: 241 QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGV 300
           QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDP+GV
Sbjct: 241 QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGV 300

Query: 301 FTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTW 360
           FTDVCIGWPGSMPDDQVLEKS+L+ERA+MGLLNDVF+VGNSGYPLMDWLLVPYTVQNLTW
Sbjct: 301 FTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPLMDWLLVPYTVQNLTW 360

Query: 361 TQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 420
           TQH FNEKVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKE
Sbjct: 361 TQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 420

Query: 421 KFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           KFDPELKFE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 421 KFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 461

BLAST of Lsi06G002980 vs. ExPASy TrEMBL
Match: A0A1S3BPN5 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103492343 PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 6.4e-231
Identity = 399/469 (85.07%), Postives = 426/469 (90.83%), Query Frame = 0

Query: 1   MDHNNT--FSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQE 60
           MD+N T  F+VN +P+KRRR D N  D   FNN SF DEN      + LLKLPCWF+ Q 
Sbjct: 24  MDNNPTTPFNVNPTPKKRRRSDPNSDD---FNNFSFTDENDEPT-DDPLLKLPCWFDPQP 83

Query: 61  ETQENWIMDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLW 120
           E+ ++W+MDS+K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLW
Sbjct: 84  ESPQSWLMDSQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNNPAGGGGTQQRRLW 143

Query: 121 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 180
           VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR
Sbjct: 144 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 203

Query: 181 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 240
           VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK
Sbjct: 204 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 263

Query: 241 QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGV 300
           QEFESISGIPKVGGSIYTTHIPIIAP+NNVAAYFNKRHTERNQKTSYSITVQGVVDP+GV
Sbjct: 264 QEFESISGIPKVGGSIYTTHIPIIAPRNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGV 323

Query: 301 FTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTW 360
           FTDVCIGWPGSMPDDQVLEKS+L+ERA+MGLLNDVF+VGNSGYPLMDWLLVPYTVQNLTW
Sbjct: 324 FTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPLMDWLLVPYTVQNLTW 383

Query: 361 TQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 420
           TQH FNEKVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKE
Sbjct: 384 TQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 443

Query: 421 KFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           KFDPELKFE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 444 KFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 484

BLAST of Lsi06G002980 vs. ExPASy TrEMBL
Match: A0A0A0LYY2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589710 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 395/462 (85.50%), Postives = 420/462 (90.91%), Query Frame = 0

Query: 6   TFSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQEETQENWI 65
           TF+VN + +KRRR D N  D   FN+ SF DEN      + LLKLPCWF+ Q E+Q+NW+
Sbjct: 30  TFNVNPTSKKRRRSDPNSDD---FNSFSFTDENDDPT-ADPLLKLPCWFDPQPESQQNWL 89

Query: 66  MDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLWVKDRSKD 125
           MD++K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLWVKDRSKD
Sbjct: 90  MDAQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNTPAGGGGTQQRRLWVKDRSKD 149

Query: 126 WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR 185
           WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR
Sbjct: 150 WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR 209

Query: 186 LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQEFESIS 245
           LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL WPDESKLTKIKQEFESIS
Sbjct: 210 LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESIS 269

Query: 246 GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIG 305
           GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDP+GVFTDVCIG
Sbjct: 270 GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIG 329

Query: 306 WPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHAFNE 365
           WPGSMPDDQVLEKS+L+ERA+MG LNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQH FNE
Sbjct: 330 WPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNE 389

Query: 366 KVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK 425
           KVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK
Sbjct: 390 KVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK 449

Query: 426 FELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           FE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 450 FEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 483

BLAST of Lsi06G002980 vs. ExPASy TrEMBL
Match: A0A6J1F642 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.3e-219
Identity = 390/468 (83.33%), Postives = 416/468 (88.89%), Query Frame = 0

Query: 1   MDHNNTFSVNQSPRKRRRQDENGVD-QSQFNNISFHDENASVNGGNDLLKLPCWFETQEE 60
           MD N  FSVNQSP+KRRRQ+++  D Q+QFN  SF D+        +LLKLP WF+  ++
Sbjct: 24  MDDN--FSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDD--------ELLKLPFWFD--DD 83

Query: 61  TQENWIMDSEKSEPRSEFHLSDQNPTQ-SSKKPRRATTENGY--PAKNAAGGAQHRRLWV 120
            Q++WIM     E + EF +SD+N TQ   KKPRRAT EN +  PAK  A G QHRRLWV
Sbjct: 84  KQQHWIM-----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAKGGA-GTQHRRLWV 143

Query: 121 KDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRV 180
           KDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDTMLRVAIPVRQRV
Sbjct: 144 KDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIPVRQRV 203

Query: 181 AVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQ 240
           AVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP+ESKL KIKQ
Sbjct: 204 AVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEESKLAKIKQ 263

Query: 241 EFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVF 300
           EFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVF
Sbjct: 264 EFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVF 323

Query: 301 TDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWT 360
           TDVCIGWPGSMPDDQVLEKS LFERANMGLL DV IVGNSGYPL DWLLVPY+ QNLTWT
Sbjct: 324 TDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQNLTWT 383

Query: 361 QHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKEK 420
           QHAFNEKV EIQ AAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKE+
Sbjct: 384 QHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKER 443

Query: 421 FDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           FDPELKFE +DDEMVPE+NG+RS +AIQARDHIAHNLLHHGLAGTGFL
Sbjct: 444 FDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGLAGTGFL 473

BLAST of Lsi06G002980 vs. ExPASy TrEMBL
Match: A0A6J1HRQ5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.6e-218
Identity = 386/467 (82.66%), Postives = 414/467 (88.65%), Query Frame = 0

Query: 1   MDHNNTFSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQEET 60
           MD N  FSVN SP+KRRRQD++  DQ+QFN  SF D+        +LLKLP WF+  ++ 
Sbjct: 24  MDDN--FSVNYSPKKRRRQDDDDDDQTQFNKTSFDDD--------ELLKLPFWFD--DDK 83

Query: 61  QENWIMDSEKSEPRSEFHLSDQNPTQ-SSKKPRRATTENGY--PAKNAAGGAQHRRLWVK 120
           Q++WIM     E + EF + D+N TQ  +KKPRRAT EN +  PAK  A G QHRRLWVK
Sbjct: 84  QQHWIM-----EQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAKGGA-GTQHRRLWVK 143

Query: 121 DRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVA 180
           DRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDTMLRVAIPVRQRVA
Sbjct: 144 DRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTVMKKDTMLRVAIPVRQRVA 203

Query: 181 VCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQE 240
           VCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP++SKL KIKQE
Sbjct: 204 VCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPEDSKLAKIKQE 263

Query: 241 FESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFT 300
           FESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFT
Sbjct: 264 FESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFT 323

Query: 301 DVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQ 360
           DVCIGWPGSMPDDQVLEKS LFERANMGLL DV IVGNSGYPL DWLLVPY+ QNLTWTQ
Sbjct: 324 DVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYPLTDWLLVPYSAQNLTWTQ 383

Query: 361 HAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKF 420
           HAFNEKV EIQ AAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKE+F
Sbjct: 384 HAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKERF 443

Query: 421 DPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           DPELKFE +DDEMVPE+NG+RS +AI ARDHI+HNLLHHGLAGTGFL
Sbjct: 444 DPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLAGTGFL 472

BLAST of Lsi06G002980 vs. NCBI nr
Match: XP_038880089.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 855.5 bits (2209), Expect = 2.1e-244
Identity = 424/467 (90.79%), Postives = 437/467 (93.58%), Query Frame = 0

Query: 2   DHNN---TFSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQE 61
           +HNN   TFSVNQSP+KRRR DENG D SQFNNISF + + +      L KLPCWF    
Sbjct: 27  NHNNTNATFSVNQSPKKRRRSDENGDDHSQFNNISFTENDEA------LQKLPCWF---- 86

Query: 62  ETQENWIMDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN-AAGGAQHRRLWVK 121
           E+QENWIMDSE+ +PR+EFHLSDQNPTQ SKKPRR T ENG PAKN   GGAQ RRLWVK
Sbjct: 87  ESQENWIMDSEEPKPRNEFHLSDQNPTQFSKKPRRTTAENGSPAKNPTGGGAQQRRLWVK 146

Query: 122 DRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVA 181
           DRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVA
Sbjct: 147 DRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVA 206

Query: 182 VCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQE 241
           VCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQE
Sbjct: 207 VCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQE 266

Query: 242 FESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFT 301
           FESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFT
Sbjct: 267 FESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFT 326

Query: 302 DVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQ 361
           DVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQ
Sbjct: 327 DVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQ 386

Query: 362 HAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKF 421
           H FNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICE+RKEKF
Sbjct: 387 HGFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEIRKEKF 446

Query: 422 DPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           DP+LKFELYDDEMVPE+NGLRSV+AIQARDHIAHNLLHHGLAGTGFL
Sbjct: 447 DPDLKFELYDDEMVPENNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 483

BLAST of Lsi06G002980 vs. NCBI nr
Match: KAA0055806.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK10057.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 810.8 bits (2093), Expect = 5.9e-231
Identity = 400/469 (85.29%), Postives = 426/469 (90.83%), Query Frame = 0

Query: 1   MDHNNT--FSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQE 60
           MD+N T  F+VN +P+KRRR D N  D   FNN SF DEN      + LLKLPCWF+ Q 
Sbjct: 1   MDNNPTTPFNVNPTPKKRRRSDPNSDD---FNNFSFTDENDEPT-DDPLLKLPCWFDPQP 60

Query: 61  ETQENWIMDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLW 120
           E+ ++W+MDS+K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLW
Sbjct: 61  ESPQSWLMDSQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNNPAGGGGTQQRRLW 120

Query: 121 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 180
           VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR
Sbjct: 121 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 180

Query: 181 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 240
           VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK
Sbjct: 181 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 240

Query: 241 QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGV 300
           QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDP+GV
Sbjct: 241 QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGV 300

Query: 301 FTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTW 360
           FTDVCIGWPGSMPDDQVLEKS+L+ERA+MGLLNDVF+VGNSGYPLMDWLLVPYTVQNLTW
Sbjct: 301 FTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPLMDWLLVPYTVQNLTW 360

Query: 361 TQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 420
           TQH FNEKVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKE
Sbjct: 361 TQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 420

Query: 421 KFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           KFDPELKFE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 421 KFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 461

BLAST of Lsi06G002980 vs. NCBI nr
Match: XP_008450882.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 809.7 bits (2090), Expect = 1.3e-230
Identity = 399/469 (85.07%), Postives = 426/469 (90.83%), Query Frame = 0

Query: 1   MDHNNT--FSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQE 60
           MD+N T  F+VN +P+KRRR D N  D   FNN SF DEN      + LLKLPCWF+ Q 
Sbjct: 24  MDNNPTTPFNVNPTPKKRRRSDPNSDD---FNNFSFTDENDEPT-DDPLLKLPCWFDPQP 83

Query: 61  ETQENWIMDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLW 120
           E+ ++W+MDS+K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLW
Sbjct: 84  ESPQSWLMDSQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNNPAGGGGTQQRRLW 143

Query: 121 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 180
           VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR
Sbjct: 144 VKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQR 203

Query: 181 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 240
           VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK
Sbjct: 204 VAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIK 263

Query: 241 QEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGV 300
           QEFESISGIPKVGGSIYTTHIPIIAP+NNVAAYFNKRHTERNQKTSYSITVQGVVDP+GV
Sbjct: 264 QEFESISGIPKVGGSIYTTHIPIIAPRNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGV 323

Query: 301 FTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTW 360
           FTDVCIGWPGSMPDDQVLEKS+L+ERA+MGLLNDVF+VGNSGYPLMDWLLVPYTVQNLTW
Sbjct: 324 FTDVCIGWPGSMPDDQVLEKSLLYERASMGLLNDVFVVGNSGYPLMDWLLVPYTVQNLTW 383

Query: 361 TQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 420
           TQH FNEKVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKE
Sbjct: 384 TQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKE 443

Query: 421 KFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           KFDPELKFE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 444 KFDPELKFEVYDDEMLPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 484

BLAST of Lsi06G002980 vs. NCBI nr
Match: XP_004144012.1 (protein ALP1-like [Cucumis sativus])

HSP 1 Score: 805.4 bits (2079), Expect = 2.5e-229
Identity = 395/462 (85.50%), Postives = 420/462 (90.91%), Query Frame = 0

Query: 6   TFSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQEETQENWI 65
           TF+VN + +KRRR D N  D   FN+ SF DEN      + LLKLPCWF+ Q E+Q+NW+
Sbjct: 30  TFNVNPTSKKRRRSDPNSDD---FNSFSFTDENDDPT-ADPLLKLPCWFDPQPESQQNWL 89

Query: 66  MDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLWVKDRSKD 125
           MD++K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLWVKDRSKD
Sbjct: 90  MDAQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNTPAGGGGTQQRRLWVKDRSKD 149

Query: 126 WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR 185
           WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR
Sbjct: 150 WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR 209

Query: 186 LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQEFESIS 245
           LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL WPDESKLTKIKQEFESIS
Sbjct: 210 LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESIS 269

Query: 246 GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIG 305
           GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDP+GVFTDVCIG
Sbjct: 270 GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIG 329

Query: 306 WPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHAFNE 365
           WPGSMPDDQVLEKS+L+ERA+MG LNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQH FNE
Sbjct: 330 WPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNE 389

Query: 366 KVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK 425
           KVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK
Sbjct: 390 KVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK 449

Query: 426 FELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           FE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 450 FEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 483

BLAST of Lsi06G002980 vs. NCBI nr
Match: KAE8653438.1 (hypothetical protein Csa_007306 [Cucumis sativus])

HSP 1 Score: 805.4 bits (2079), Expect = 2.5e-229
Identity = 395/462 (85.50%), Postives = 420/462 (90.91%), Query Frame = 0

Query: 6   TFSVNQSPRKRRRQDENGVDQSQFNNISFHDENASVNGGNDLLKLPCWFETQEETQENWI 65
           TF+VN + +KRRR D N  D   FN+ SF DEN      + LLKLPCWF+ Q E+Q+NW+
Sbjct: 101 TFNVNPTSKKRRRSDPNSDD---FNSFSFTDENDDPT-ADPLLKLPCWFDPQPESQQNWL 160

Query: 66  MDSEKSEPRSEFHLSDQNPTQSSKKPRRATTENGYPAKN---AAGGAQHRRLWVKDRSKD 125
           MD++K +P ++FHLSDQ P    KKPRRA+ EN  P KN     GG Q RRLWVKDRSKD
Sbjct: 161 MDAQKPKPTNDFHLSDQIP----KKPRRASPENPSPVKNTPAGGGGTQQRRLWVKDRSKD 220

Query: 126 WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR 185
           WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR
Sbjct: 221 WWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWR 280

Query: 186 LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESKLTKIKQEFESIS 245
           LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFL WPDESKLTKIKQEFESIS
Sbjct: 281 LATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLNWPDESKLTKIKQEFESIS 340

Query: 246 GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPAGVFTDVCIG 305
           GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDP+GVFTDVCIG
Sbjct: 341 GIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIG 400

Query: 306 WPGSMPDDQVLEKSVLFERANMGLLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHAFNE 365
           WPGSMPDDQVLEKS+L+ERA+MG LNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQH FNE
Sbjct: 401 WPGSMPDDQVLEKSLLYERASMGSLNDVFIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNE 460

Query: 366 KVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK 425
           KVGEIQAAAK AFGRLKGRW+CLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK
Sbjct: 461 KVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCVLHNICEMRKEKFDPELK 520

Query: 426 FELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAGTGFL 465
           FE+YDDEM+PE+NGLRSV+AIQARDHIAHNLLHHG+AGTGFL
Sbjct: 521 FEVYDDEMMPENNGLRSVSAIQARDHIAHNLLHHGIAGTGFL 554

BLAST of Lsi06G002980 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 572.4 bits (1474), Expect = 3.3e-163
Identity = 268/367 (73.02%), Postives = 317/367 (86.38%), Query Frame = 0

Query: 99  GYPAKNAAGGAQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDST 158
           G  +    G  Q RRLWVKDRS+ WW++C+  D+P+E+F++AFRMSKSTF++IC EL+S 
Sbjct: 137 GSGSVRGTGSGQQRRLWVKDRSRAWWEECSRLDYPEEDFKKAFRMSKSTFELICDELNSA 196

Query: 159 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 218
           V K+DT LR AIPVRQRVAVCIWRLATGEPLRLVSK+FGLGISTCHKLVLEVC AI+ VL
Sbjct: 197 VAKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVL 256

Query: 219 MPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 278
           MPK+LQWPD+  L  I++ FES+SGIP V GS+YTTHIPIIAPK +VA+YFNKRHTERNQ
Sbjct: 257 MPKYLQWPDDESLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQ 316

Query: 279 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANM-GLLNDVFIVGNSG 338
           KTSYSIT+Q VV+P GVFTD+CIGWPGSMPDD+VLEKS+L++RAN  GLL  +++ G  G
Sbjct: 317 KTSYSITIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGMWVAGGPG 376

Query: 339 YPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELP 398
           +PL+DW+LVPYT QNLTWTQHAFNEK+ E+Q  AK AFGRLKGRW+CLQKRTEVKLQ+LP
Sbjct: 377 HPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQKRTEVKLQDLP 436

Query: 399 VVLGACCVLHNICEMRKEKFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHG 458
            VLGACCVLHNICEMR+EK +PEL  E+ DDE++PE N LRSVNA++ARD I+HNLLHHG
Sbjct: 437 TVLGACCVLHNICEMREEKMEPELMVEVIDDEVLPE-NVLRSVNAMKARDTISHNLLHHG 496

Query: 459 LAGTGFL 465
           LAGT FL
Sbjct: 497 LAGTSFL 502

BLAST of Lsi06G002980 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 537.7 bits (1384), Expect = 9.0e-153
Identity = 258/361 (71.47%), Postives = 301/361 (83.38%), Query Frame = 0

Query: 102 AKNAAGGAQHRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMK 161
           A  +  G  HRRLWVK+R+ DWWD+ + PDFP++EFRR FRMSKSTF++IC+ELD+TV K
Sbjct: 178 ASGSGSGPSHRRLWVKERTTDWWDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTK 237

Query: 162 KDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPK 221
           K+TMLR AIP  +RV VC+WRLATG PLR VS+RFGLGISTCHKLV+EVC AI  VLMPK
Sbjct: 238 KNTMLRDAIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPK 297

Query: 222 FLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTS 281
           +L WP +S++   K +FES+  IP V GSIYTTHIPIIAPK +VAAYFNKRHTERNQKTS
Sbjct: 298 YLLWPSDSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTS 357

Query: 282 YSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLF-ERANMGLLNDVFIVGNSGYPL 341
           YSITVQGVV+  G+FTDVCIG PGS+ DDQ+LEKS L  +RA  G+L D +IVGNSG+PL
Sbjct: 358 YSITVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSWIVGNSGFPL 417

Query: 342 MDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVL 401
            D+LLVPYT QNLTWTQHAFNE +GEIQ  A  AF RLKGRW+CLQKRTEVKLQ+LP VL
Sbjct: 418 TDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVL 477

Query: 402 GACCVLHNICEMRKEKFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAHNLLHHGLAG 461
           GACCVLHNICEMRKE+  PELKFE++DD  VPE+N +RS +A+  RDHI+HNLLH GLAG
Sbjct: 478 GACCVLHNICEMRKEEMLPELKFEVFDDVAVPENN-IRSASAVNTRDHISHNLLHRGLAG 537

BLAST of Lsi06G002980 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-38
Identity = 112/362 (30.94%), Postives = 174/362 (48.07%), Query Frame = 0

Query: 119 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 178
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 179 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDESK 238
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 239 LTKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 298
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 299 QGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMG-LLND------------VFIV 358
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 359 GNSGYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEV-K 418
           G+SG+PL+ WLL PY  +  +  Q  FN++  E   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 419 LQELPVVLGACCVLHN-ICEMRKEKFDPELKFELYDDEMVPESNGLRSVNAIQARDHIAH 454
              LP ++  CC+LHN I +M  +  D +   + +D      S  L    +   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of Lsi06G002980 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-35
Identity = 95/314 (30.25%), Postives = 158/314 (50.32%), Query Frame = 0

Query: 122 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 181
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 182 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDES 241
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 242 KLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 301
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 302 VDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLND-------------VFIVGNS 361
            D    F ++  GWPG M   ++L+ S  F+      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 362 GYPLMDWLLVPYTVQNLTWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQK-RTEVKLQE 410
            YPL+ WL+ P+   + + +  AFNE+  ++++ A TAF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Lsi06G002980 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-22
Identity = 79/309 (25.57%), Postives = 145/309 (46.93%), Query Frame = 0

Query: 134 DEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVS 193
           D  +R  + +S   F  +  +L   +    T   +++P    VA+ + RLA G   + ++
Sbjct: 114 DARWRSLYGLSYPVFITVVDKLKPFI----TASNLSLPADYAVAMVLSRLAHGCSAKTLA 173

Query: 194 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP-DESKLTKIKQEFESISGIPKVGGSIY 253
            R+ L      K+   V   +   L P+F++ P  + +L +  Q FE ++ +P + G+I 
Sbjct: 174 SRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAID 233

Query: 254 TTHIPIIAPKNNVAAYFNKRHTERNQKTSY-------SITVQGVVDPAGVFTDVCIGWPG 313
           +T + +            +R T+ N +  Y       ++ +Q V D   +F DVC+  PG
Sbjct: 234 STPVKL------------RRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPG 293

Query: 314 SMPDDQVLEKSVLFERANMG------LLN------DVFIVGNSGYPLMDWLLVPYTVQNL 373
              D      S+L++R   G      ++N        +IVG+  YPL+ +L+ P++    
Sbjct: 294 GEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGS 353

Query: 374 -TWTQHAFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEM 422
            T  ++ F+  + + ++    A G LK RW  LQ    V +   P  + ACCVLHN+C++
Sbjct: 354 GTPPENLFDGMLMKGRSVVVEAIGLLKARWKILQS-LNVGVNHAPQTIVACCVLHNLCQI 404