Lsi06G002300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G002300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDerlin
Locationchr06: 2639923 .. 2643670 (-)
RNA-Seq ExpressionLsi06G002300
SyntenyLsi06G002300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTCGGTCGTATTTGGTTTCCTTCGCCCTTCCATTCTGCAATTTCCATTGTCATCTTCCAATTTCTTGTTTGCTAAGTTTTGTTCCTCTTCTGGTGTTGAAATTTCTTTAAGAAGAACTGAGAGATCTCCACTTCCAAAGCTTCTTTGAATTTGATTTCTGTTTCTACAATGTCTTCTCCTGCCGAGTAATTCTGCTTTTCCTCTTTGAATTTTACGAATTTTGTATTTCATTTTTATTAGCTTTCGATTGGTCTGTTTCATGTTCTAATTTTCTCATTTTCAATGTCTTCTCTGATTCTTTACTCTCATTTTAATCTGTATCCTGAGTTCTGATCATTTTTATCATCATTCAGATTTTACCACTCTCTTCCACCTATAAGTAAGGCGTATGGAACTCTGTGTCTTTTGGCAACTACAGCCTTCCAACTTGGCTTGTACGATCCCGGGCTTATTGCGTTGGATTACGGACTTGTATTCAAGCATTTTCAGGTATTGACTTCAATCTCCTTAGGTATCAGAATGGTGCTCCTATATATTTGTTGAATTTTTTACTTTCCTCAAACATCTGCTGGCTCCTACTTCCCTTAGGGCTTTTATCTTTCGAAGGGACATAAAATCTACTAGTTTGAGTGATATAGAAATCTAGTTCTTATCTTCTCGACTGATAATAATATGGAGTTGATATAATATCAGATATAGCTCCGAGTATGTACTTATGAATGAAGGTGCAATTTAGAACTAATTTTCAGGACCATCGGGAACATCTTTAACTTAACTTTTGAGGTCATTTTTGAAGCCTATGATAGTGACTTATAGCCAATTTTCAGTTCAAGCTCCTCTTATAATTAATACTAAGTGTAATTGATGTGGTTGAGGCAGTCAACTCATTACTGATAATGACTTTAGCCAATTTTTTAAGCCTCTGATAGTGACTTTAGACAATTTTCAGTTTAACTTAACTTTGGTCCATTTAGGATGTTAAATGTTATACAAATTCTTTTTATGTCAAATCTTGTTGGGTAAGGCCGTCATCTCATCGTATTGCTTGAGACACAATGTAAAATTTCCTCCCCTAGTATCAATCTGCTCTCTCATACTTGTTCTTTATATTCTAAAATTAATTAATATTAGAAAGCTCTATTTCAAAGATTTGGGTTAGAACTAGATTTAGGACCAGTTCAATTATGAATCATTTTTCTTGATGTCATGGTCTCTCTACATGACGCCTAATGGTCAACCCTTTTATCAATAATGAAGTGAACTGTGGTAAAGGCAAGTAAAAGTAGTGAAGGAGTGTGATGTAATGGGGGATATCACATGAAGCCCAAGTCAGGAAAAATCATGGGCAGAACCAACTTGATTTATGCTTTGGATTTTAAATTCTAAAGTGAAAAGAATTAGCTATTGTTATTTCTGATTTCTAATTCCAATGGAAATTATCGATTCTCATCAAGGGAATTGTTCTTATACAAGAAAAGTGGTACTGTGCAAAGAGAATGGTATAAGTTTGCTCTCTCTCTCTCTCTCTGGTAATTCCCTTGTATTTATGACGTTCTTTTACCTTTGGACCGAATATTTTAGATGATCCTGGAGATGGATGCCTTTCCCTTTTCACTAATTCTTGAATGTGAGTTCCACCACATTATTTCCAACTATTGTTATTACTATTTATGCAAGGTCACCTTTATTTTCAGGTGTGGAGGCTATTCACAAACTTCTTCTTCCTTGGAAAATTCTCTATCAATTTTGGAATTCGTTTATTAATGATGTAAGTTTTCTTGTGTGGTAACTTTTTACGTGAACTACTGGCAGTACTATTTCATGAAAAGTGGAGGGCTTGTGTTCAAATTTCAAACTCTCATGATGGTTTATGATGCACTAATGGTTTCATGGTTGTGCCCTTACAGAGCAAGATATGGGGTTCAACTTGAGAATGGACCATTTCAGAGGCGAACAGCGGATTTTCTGTGGATGATGATATTTGGAGCCTTGTCATTATTGGTTTGTGAGGATCTTTATTAAGCTATTCCTTTAGATGCAGTGGTTGTTGCTGGCCTTTGTTTGACCTTTTTGTATTCTTTCATTTCTCTTCTCAAGGTTTGGTTTTTGGATAAAAAATTATATGCCAGTGGTTCTTAAGTCTTATTTATTTGTAAAAATAGTAGATTTTGCAGATGGCGCCTAATGGTTTTTATATTCTTTTGCCAATCCTTGTTCATTTGCAAGTCTATGAAGTAAAATCATCAGTTTGAGTAGAAAATTATTAGGTAGTCCTATATTTCATCATCGCCACAGTACCACCAAAAATGTTTTGGATTTGGTATAAATATTTCATTGAGTTACTAGTAGGTGAGTATTTTCCAACAACCTCGACTTTTTCGTATTTCTGTTTCTCTGGGAACCTTTTTCTCATGAAATCCTCCCATCTGAGTTTTTGGTTGTTGAGTCTTTATTTTAGGTTGAATATTTATGCCGATATGTTAACGCCTTTCTTATTATGTATACTTTCTCTTGAATAGGTACTTTCTGCTATCCCAATCTTCACGTTTCCAGTCCTAGGGATATCACTTGTGTTTATGCTTCTTTACGTCTGGAGTAGAGAATTTCCTAATGCACAGATCAATATATATGGCCTTGTGACTCTTAAGGTATGGCATTGATTTAGGTTTTGTCAAATTGTTCCTTTTTTCCCCCTAGGATTGTGTTCTCAGTTCCTGATATCTGCACAACACAAATGTAATGCTTTTTATGCAGGCATTTTATCTGCCATGGGCCATGCTAGCGCTGGATGTCATTTTTGGTTCACCTCTTGTCCCTGATTTACTCGGAATCCTAGCAGGGCATTTGTACTACTTTTTGACTGTGTTGCATCCACTGGCAGGCGGAAAGAACATATTGAGAACTCCATTTTGGATGTATCCTTCCTCTTTTGGTTTGTTAACAACAAAATTTTCTTGATTCTGCCTATGCATCTATAATTTCATATCCCCATCGCTCTGCCTATTTCACTTATAATCTTCCATTTCCAAAACAGTAAAATTTATAACAAATGCTTCCCCTCCAACTAAACAGTCATACACTTTGCGGTATGATCTTATTCACCAAAATGCATTTTCATCATACTCACCTGTTTTGGTTTTTTCTATTGTTTGGGTTGCTTTATTCTGCATTCCTTTAACAATTTCTACAGACAGAGGTTAGTCTCAAGGTGGAGGATAGGTGCTCCTCCAATTCAACGTGCGGCGGTGCCTGATGATAGGGGTACGAGCGGAGCTTTCAGAGGGAGATCTTATCGGCTTAATGGCTAAACTCATTTCTTGTACAAGAAAACAAAAAATGGCCATCCTATTAACACTGGAGTTTTTCAGATTGTGCCTGCTGACATGAAGGTAAAGATTGGGTTGAAGAATTTGGTTAGTTTCGAAAAAGTTGGTCTCGAGCGAATATTTGTTTCATGCGTAGTGATCATTAAGTGCTTATGTTTCTTGTTGAAGGAATTTGGATTCGGAAATTTTGTTCTGCAGGAGATAAAAATCGGGATAATCTTGACTCTTGAGTTTCGCTTTGAGAGACTTCTTCTGAACTCTGTAATCGAATAAAATCCCAACTTTCCCAATCCTTATGATTAGAAATACCAGAGATTGTTTTTCCCCTCCTTTTTGTCGACATTTTGAAATTTAAAATGTAGGTTTATGATCTCTTAAGTACAATGTGATTACTGATTAATGATGGGGTATTGAGTTTT

mRNA sequence

CTTCTCGGTCGTATTTGGTTTCCTTCGCCCTTCCATTCTGCAATTTCCATTGTCATCTTCCAATTTCTTGTTTGCTAAGTTTTGTTCCTCTTCTGGTGTTGAAATTTCTTTAAGAAGAACTGAGAGATCTCCACTTCCAAAGCTTCTTTGAATTTGATTTCTGTTTCTACAATGTCTTCTCCTGCCGAATTTTACCACTCTCTTCCACCTATAAGTAAGGCGTATGGAACTCTGTGTCTTTTGGCAACTACAGCCTTCCAACTTGGCTTGTACGATCCCGGGCTTATTGCGTTGGATTACGGACTTGTATTCAAGCATTTTCAGGATGTTAAATGTTATACAAATTCTTTTTATGTCAAATCTTGTTGGAAAGCTCTATTTCAAAGATTTGGGCAAGTAAAAGTAGTGAAGGAGTGTGATGTGTGGAGGCTATTCACAAACTTCTTCTTCCTTGGAAAATTCTCTATCAATTTTGGAATTCGTTTATTAATGATAGCAAGATATGGGGTTCAACTTGAGAATGGACCATTTCAGAGGCGAACAGCGGATTTTCTGTGGATGATGATATTTGGAGCCTTGTCATTATTGGTACTTTCTGCTATCCCAATCTTCACGTTTCCAGTCCTAGGGATATCACTTGTGTTTATGCTTCTTTACGTCTGGAGTAGAGAATTTCCTAATGCACAGATCAATATATATGGCCTTGTGACTCTTAAGGCATTTTATCTGCCATGGGCCATGCTAGCGCTGGATGTCATTTTTGGTTCACCTCTTGTCCCTGATTTACTCGGAATCCTAGCAGGGCATTTGTACTACTTTTTGACTGTGTTGCATCCACTGGCAGGCGGAAAGAACATATTGAGAACTCCATTTTGGATGTATCCTTCCTCTTTTGTAAAATTTATAACAAATGCTTCCCCTCCAACTAAACAGTCATACACTTTGCGACAGAGGTTAGTCTCAAGGTGGAGGATAGGTGCTCCTCCAATTCAACGTGCGGCGGTGCCTGATGATAGGGGTACGAGCGGAGCTTTCAGAGGGAGATCTTATCGGCTTAATGGCTAAACTCATTTCTTGTACAAGAAAACAAAAAATGGCCATCCTATTAACACTGGAGTTTTTCAGATTGTGCCTGCTGACATGAAGGAATTTGGATTCGGAAATTTTGTTCTGCAGGAGATAAAAATCGGGATAATCTTGACTCTTGAGTTTCGCTTTGAGAGACTTCTTCTGAACTCTGTAATCGAATAAAATCCCAACTTTCCCAATCCTTATGATTAGAAATACCAGAGATTGTTTTTCCCCTCCTTTTTGTCGACATTTTGAAATTTAAAATGTAGGTTTATGATCTCTTAAGTACAATGTGATTACTGATTAATGATGGGGTATTGAGTTTT

Coding sequence (CDS)

ATGTCTTCTCCTGCCGAATTTTACCACTCTCTTCCACCTATAAGTAAGGCGTATGGAACTCTGTGTCTTTTGGCAACTACAGCCTTCCAACTTGGCTTGTACGATCCCGGGCTTATTGCGTTGGATTACGGACTTGTATTCAAGCATTTTCAGGATGTTAAATGTTATACAAATTCTTTTTATGTCAAATCTTGTTGGAAAGCTCTATTTCAAAGATTTGGGCAAGTAAAAGTAGTGAAGGAGTGTGATGTGTGGAGGCTATTCACAAACTTCTTCTTCCTTGGAAAATTCTCTATCAATTTTGGAATTCGTTTATTAATGATAGCAAGATATGGGGTTCAACTTGAGAATGGACCATTTCAGAGGCGAACAGCGGATTTTCTGTGGATGATGATATTTGGAGCCTTGTCATTATTGGTACTTTCTGCTATCCCAATCTTCACGTTTCCAGTCCTAGGGATATCACTTGTGTTTATGCTTCTTTACGTCTGGAGTAGAGAATTTCCTAATGCACAGATCAATATATATGGCCTTGTGACTCTTAAGGCATTTTATCTGCCATGGGCCATGCTAGCGCTGGATGTCATTTTTGGTTCACCTCTTGTCCCTGATTTACTCGGAATCCTAGCAGGGCATTTGTACTACTTTTTGACTGTGTTGCATCCACTGGCAGGCGGAAAGAACATATTGAGAACTCCATTTTGGATGTATCCTTCCTCTTTTGTAAAATTTATAACAAATGCTTCCCCTCCAACTAAACAGTCATACACTTTGCGACAGAGGTTAGTCTCAAGGTGGAGGATAGGTGCTCCTCCAATTCAACGTGCGGCGGTGCCTGATGATAGGGGTACGAGCGGAGCTTTCAGAGGGAGATCTTATCGGCTTAATGGCTAA

Protein sequence

MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSFYVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPFQRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVTLKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSSFVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG
Homology
BLAST of Lsi06G002300 vs. ExPASy Swiss-Prot
Match: Q8VZU9 (Derlin-1 OS=Arabidopsis thaliana OX=3702 GN=DER1 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.0e-85
Identity = 182/319 (57.05%), Postives = 200/319 (62.70%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSP EFY+SLPPI+KAYGTLC   T A QLGL  P  IAL   LV K FQ         
Sbjct: 1   MSSPGEFYNSLPPITKAYGTLCFFTTVATQLGLVAPVHIALIPELVLKQFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  +WRL TN FFLG FSINFGIRLLMIARYGVQLE GPF
Sbjct: 61  -----------------------IWRLITNLFFLGGFSINFGIRLLMIARYGVQLEKGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           +RRTADFLWMMIFG+ +LLVLS IP F  P LG+SLVFMLLY+WSREFPNA I++YGLVT
Sbjct: 121 ERRTADFLWMMIFGSFTLLVLSVIPFFWTPFLGVSLVFMLLYLWSREFPNANISLYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSP++PDLLGI+AGHLYYFLTVLHPLA GKN L+TP W+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPIMPDLLGIIAGHLYYFLTVLHPLATGKNYLKTPKWV---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQ-------RAAVPDDRGTSG------- 296
                               ++V+RWRIGAP           AA P   G  G       
Sbjct: 241 -------------------NKIVARWRIGAPVASVRQAGGVGAAGPGAGGGVGGGGAYSS 264

BLAST of Lsi06G002300 vs. ExPASy Swiss-Prot
Match: Q06397 (Derlin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=DER1 PE=2 SV=2)

HSP 1 Score: 303.1 bits (775), Expect = 3.4e-81
Identity = 167/299 (55.85%), Postives = 198/299 (66.22%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAE+Y+SLPPISKAYGTLC  AT   QL + +P  +AL Y  VFK FQ         
Sbjct: 1   MSSPAEYYNSLPPISKAYGTLCFFATVLCQLQILNPPFLALYYPFVFKKFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  +WRLFT+FFFLGKFSINFGIRLLMIARYGVQLE G F
Sbjct: 61  -----------------------IWRLFTSFFFLGKFSINFGIRLLMIARYGVQLEKGAF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           ++RTADFLWMMIFGA+SLL LSAIP      LG+ +V MLLYVWSRE+PN+QI++YGLV 
Sbjct: 121 EKRTADFLWMMIFGAISLLALSAIPFLDIYFLGVPMVSMLLYVWSREYPNSQISMYGLVQ 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           L++FYLPWAML LDVIFGS ++P LLGIL GH YYFL+VLHPLA GKN L+TP W++   
Sbjct: 181 LRSFYLPWAMLGLDVIFGSEILPGLLGILVGHTYYFLSVLHPLATGKNYLKTPMWVH--- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGA---PPIQRAAVPDDRGTSGAFRGRSYRLN 297
                               ++V+R+RIG     P++ AA       SGAFRGRSYRL+
Sbjct: 241 --------------------KIVARFRIGVQANAPVRPAAA---NTGSGAFRGRSYRLS 241

BLAST of Lsi06G002300 vs. ExPASy Swiss-Prot
Match: Q4G2J5 (Derlin-1.2 OS=Zea mays OX=4577 GN=DER1.2 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.3e-77
Identity = 165/298 (55.37%), Postives = 191/298 (64.09%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAE+Y SLPPISKAYGTLC   T   +L + +P  + L Y  VFK F          
Sbjct: 1   MSSPAEYYKSLPPISKAYGTLCFFTTVLVRLHILNPLFLYLYYPRVFKKF---------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                 +VWR+FT+FFFLG FSINFGIRLLMIARYGV LE G F
Sbjct: 61  ----------------------EVWRIFTSFFFLGPFSINFGIRLLMIARYGVMLEKGAF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
            +RTADFLWMMIFGA+SLLVLS IP     VLG+ +V ML+YVWSRE PNAQINIYG++ 
Sbjct: 121 DKRTADFLWMMIFGAISLLVLSVIPQLNTYVLGLPMVSMLVYVWSRENPNAQINIYGILQ 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPW ML LDVIFGSPL+P LLGI+ GHLYY+  VLHPLA GKN L+TP W++   
Sbjct: 181 LKAFYLPWVMLLLDVIFGSPLMPGLLGIMVGHLYYYFAVLHPLATGKNYLKTPKWVH--- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIG--APPIQRAAVPDDRGTSGAFRGRSYRLN 297
                               ++V+R+RIG  A    RA    + GT GAFRGRSYRLN
Sbjct: 241 --------------------KIVARFRIGMQANAPVRAPANGNAGT-GAFRGRSYRLN 242

BLAST of Lsi06G002300 vs. ExPASy Swiss-Prot
Match: Q4G2J6 (Derlin-1.1 OS=Zea mays OX=4577 GN=DER1.1 PE=2 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 6.6e-77
Identity = 162/297 (54.55%), Postives = 186/297 (62.63%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAE+Y SLPPISKAYGTLC   T   QL +  P  + LDY LVFK F          
Sbjct: 1   MSSPAEYYKSLPPISKAYGTLCFFTTVLVQLQILHPLFLYLDYPLVFKKF---------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                 ++WRL T+FFFL  FS+ FGIRLLMIARYGV LE G F
Sbjct: 61  ----------------------EIWRLLTSFFFLAPFSMKFGIRLLMIARYGVMLEKGAF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
            +RTADFLWMMIFGA+SLLVLS IP+F    LGI +V MLLYVWSRE PNAQINIYGLV 
Sbjct: 121 DKRTADFLWMMIFGAISLLVLSIIPLFNSFFLGIPMVSMLLYVWSRENPNAQINIYGLVQ 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           L++FYLPWAML LDVIFGS L+P LLGI+ GHLYYF  VLHPLA GK+ L+TP W++   
Sbjct: 181 LRSFYLPWAMLLLDVIFGSSLMPGLLGIMVGHLYYFFAVLHPLATGKSYLKTPKWVH--- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVP-DDRGTSGAFRGRSYRLN 297
                               ++V+R+RIG         P +    SG FRGRSYRLN
Sbjct: 241 --------------------KIVARFRIGMQANSPVRPPANGNSGSGVFRGRSYRLN 242

BLAST of Lsi06G002300 vs. ExPASy Swiss-Prot
Match: Q96Q80 (Derlin-3 OS=Homo sapiens OX=9606 GN=DERL3 PE=1 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 9.0e-34
Identity = 88/245 (35.92%), Postives = 130/245 (53.06%), Query Frame = 0

Query: 5   AEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSFYVKS 64
           AEF   +P +++AY   C+L T A QL L  P  +  +  LVF+ FQ             
Sbjct: 8   AEFL-QVPAVTRAYTAACVLTTAAVQLELLSPFQLYFNPHLVFRKFQ------------- 67

Query: 65  CWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPFQRRT 124
                              VWRL TNF F G    +F   +L + RY   LE G F+ RT
Sbjct: 68  -------------------VWRLVTNFLFFGPLGFSFFFNMLFVFRYCRMLEEGSFRGRT 127

Query: 125 ADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVTLKAF 184
           ADF++M +FG + + +L  +    F  LG +L+ ML+YVWSR  P  ++N +GL+T +A 
Sbjct: 128 ADFVFMFLFGGVLMTLLGLLGSLFF--LGQALMAMLVYVWSRRSPRVRVNFFGLLTFQAP 187

Query: 185 YLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHP-LAGGKNILRTPFWMYPSSFVK 244
           +LPWA++   ++ G+ ++ DLLGI  GH+YYFL  + P   GGK +L+TP       F+K
Sbjct: 188 FLPWALMGFSLLLGNSILVDLLGIAVGHIYYFLEDVFPNQPGGKRLLQTP------GFLK 211

Query: 245 FITNA 249
            + +A
Sbjct: 248 LLLDA 211

BLAST of Lsi06G002300 vs. ExPASy TrEMBL
Match: A0A5D3CDT1 (Derlin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002800 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 5.9e-121
Identity = 234/297 (78.79%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGTLCLLATTAFQLGLYDP LIALDYGLVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTLCLLATTAFQLGLYDPMLIALDYGLVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFG L+LLVL+AIPIF FPVLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGGLTLLVLAAIPIFRFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWI---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG 298
                              QRLVSRWRIGAPP+QRAAVPDDRGTSGAFRGRSYRLNG
Sbjct: 241 -------------------QRLVSRWRIGAPPMQRAAVPDDRGTSGAFRGRSYRLNG 242

BLAST of Lsi06G002300 vs. ExPASy TrEMBL
Match: A0A1S3BQ27 (Derlin OS=Cucumis melo OX=3656 GN=LOC103492274 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 5.9e-121
Identity = 234/297 (78.79%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGTLCLLATTAFQLGLYDP LIALDYGLVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTLCLLATTAFQLGLYDPMLIALDYGLVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFG L+LLVL+AIPIF FPVLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGGLTLLVLAAIPIFRFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWI---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG 298
                              QRLVSRWRIGAPP+QRAAVPDDRGTSGAFRGRSYRLNG
Sbjct: 241 -------------------QRLVSRWRIGAPPMQRAAVPDDRGTSGAFRGRSYRLNG 242

BLAST of Lsi06G002300 vs. ExPASy TrEMBL
Match: A0A0A0LZA3 (Derlin OS=Cucumis sativus OX=3659 GN=Csa_1G575080 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.3e-120
Identity = 233/297 (78.45%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGTLC LATTAFQLGLYDP LIALDYGLVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTLCFLATTAFQLGLYDPMLIALDYGLVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFG+L+LLVL+AIPIF FPVLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGSLTLLVLAAIPIFQFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWI---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG 298
                              QRLVSRWRIGAPP+QRAAVPDDRGTSGAFRGRSYRLNG
Sbjct: 241 -------------------QRLVSRWRIGAPPMQRAAVPDDRGTSGAFRGRSYRLNG 242

BLAST of Lsi06G002300 vs. ExPASy TrEMBL
Match: A0A6J1HA50 (Derlin OS=Cucurbita moschata OX=3662 GN=LOC111461868 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.0e-117
Identity = 231/296 (78.04%), Postives = 237/296 (80.07%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGT+CLLATTAFQLGLYDPGLIALDY LVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTMCLLATTAFQLGLYDPGLIALDYELVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFGAL+LLVLSAIPIF+F VLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGALTLLVLSAIPIFSFSVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWV---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLN 297
                              QR+VSRWRIGAPPIQR AVPDDR TSGAFRGRSYRLN
Sbjct: 241 -------------------QRIVSRWRIGAPPIQR-AVPDDRRTSGAFRGRSYRLN 240

BLAST of Lsi06G002300 vs. ExPASy TrEMBL
Match: A0A6J1JBH3 (Derlin OS=Cucurbita maxima OX=3661 GN=LOC111485268 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 4.0e-117
Identity = 230/296 (77.70%), Postives = 236/296 (79.73%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGT+CLLATTAFQLGLYDPGLIALDY LVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTMCLLATTAFQLGLYDPGLIALDYELVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLG FSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGNFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFGAL+LLVLSAIPIF+F VLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGALTLLVLSAIPIFSFSVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWV---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLN 297
                              QR+VSRWRIGAPPIQR AVPDDR TSGAFRGRSYRLN
Sbjct: 241 -------------------QRIVSRWRIGAPPIQR-AVPDDRRTSGAFRGRSYRLN 240

BLAST of Lsi06G002300 vs. NCBI nr
Match: XP_038878469.1 (derlin-1 [Benincasa hispida])

HSP 1 Score: 448.7 bits (1153), Expect = 3.8e-122
Identity = 236/297 (79.46%), Postives = 242/297 (81.48%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGTLCLLATTAFQLGLY+PGLIALDYGLVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTLCLLATTAFQLGLYNPGLIALDYGLVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFGAL+LLVLSAIPIF+FPVLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGALTLLVLSAIPIFSFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWI---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG 298
                              QRLVSRWRIGAPPIQRAAVPDDRGT+GAFRGRSYRLNG
Sbjct: 241 -------------------QRLVSRWRIGAPPIQRAAVPDDRGTTGAFRGRSYRLNG 242

BLAST of Lsi06G002300 vs. NCBI nr
Match: XP_008450797.1 (PREDICTED: derlin-1 [Cucumis melo] >TYK10137.1 derlin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-120
Identity = 234/297 (78.79%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGTLCLLATTAFQLGLYDP LIALDYGLVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTLCLLATTAFQLGLYDPMLIALDYGLVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFG L+LLVL+AIPIF FPVLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGGLTLLVLAAIPIFRFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWI---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG 298
                              QRLVSRWRIGAPP+QRAAVPDDRGTSGAFRGRSYRLNG
Sbjct: 241 -------------------QRLVSRWRIGAPPMQRAAVPDDRGTSGAFRGRSYRLNG 242

BLAST of Lsi06G002300 vs. NCBI nr
Match: XP_004135679.1 (derlin-1 [Cucumis sativus] >KGN66192.1 hypothetical protein Csa_007006 [Cucumis sativus])

HSP 1 Score: 442.6 bits (1137), Expect = 2.7e-120
Identity = 233/297 (78.45%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGTLC LATTAFQLGLYDP LIALDYGLVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTLCFLATTAFQLGLYDPMLIALDYGLVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFG+L+LLVL+AIPIF FPVLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGSLTLLVLAAIPIFQFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWI---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLNG 298
                              QRLVSRWRIGAPP+QRAAVPDDRGTSGAFRGRSYRLNG
Sbjct: 241 -------------------QRLVSRWRIGAPPMQRAAVPDDRGTSGAFRGRSYRLNG 242

BLAST of Lsi06G002300 vs. NCBI nr
Match: XP_022961351.1 (derlin-1-like [Cucurbita moschata] >XP_023516821.1 derlin-1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516822.1 derlin-1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 433.0 bits (1112), Expect = 2.2e-117
Identity = 231/296 (78.04%), Postives = 237/296 (80.07%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSPAEFY+SLPPISKAYGT+CLLATTAFQLGLYDPGLIALDY LVFKHFQ         
Sbjct: 1   MSSPAEFYNSLPPISKAYGTMCLLATTAFQLGLYDPGLIALDYELVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFGAL+LLVLSAIPIF+F VLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGALTLLVLSAIPIFSFSVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWV---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLN 297
                              QR+VSRWRIGAPPIQR AVPDDR TSGAFRGRSYRLN
Sbjct: 241 -------------------QRIVSRWRIGAPPIQR-AVPDDRRTSGAFRGRSYRLN 240

BLAST of Lsi06G002300 vs. NCBI nr
Match: KAG7023624.1 (Derlin-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.8 bits (1109), Expect = 4.8e-117
Identity = 230/296 (77.70%), Postives = 237/296 (80.07%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MS+PAEFY+SLPPISKAYGT+CLLATTAFQLGLYDPGLIALDY LVFKHFQ         
Sbjct: 1   MSTPAEFYNSLPPISKAYGTMCLLATTAFQLGLYDPGLIALDYELVFKHFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF
Sbjct: 61  -----------------------VWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           QRRTADFLWMMIFGAL+LLVLSAIPIF+F VLGISLVFMLLYVWSREFPNAQINIYGLVT
Sbjct: 121 QRRTADFLWMMIFGALTLLVLSAIPIFSFSVLGISLVFMLLYVWSREFPNAQINIYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFW+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWV---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQRAAVPDDRGTSGAFRGRSYRLN 297
                              QR+VSRWRIGAPPIQR AVPDDR TSGAFRGRSYRLN
Sbjct: 241 -------------------QRIVSRWRIGAPPIQR-AVPDDRRTSGAFRGRSYRLN 240

BLAST of Lsi06G002300 vs. TAIR 10
Match: AT4G29330.1 (DERLIN-1 )

HSP 1 Score: 316.6 bits (810), Expect = 2.1e-86
Identity = 182/319 (57.05%), Postives = 200/319 (62.70%), Query Frame = 0

Query: 1   MSSPAEFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSF 60
           MSSP EFY+SLPPI+KAYGTLC   T A QLGL  P  IAL   LV K FQ         
Sbjct: 1   MSSPGEFYNSLPPITKAYGTLCFFTTVATQLGLVAPVHIALIPELVLKQFQ--------- 60

Query: 61  YVKSCWKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPF 120
                                  +WRL TN FFLG FSINFGIRLLMIARYGVQLE GPF
Sbjct: 61  -----------------------IWRLITNLFFLGGFSINFGIRLLMIARYGVQLEKGPF 120

Query: 121 QRRTADFLWMMIFGALSLLVLSAIPIFTFPVLGISLVFMLLYVWSREFPNAQINIYGLVT 180
           +RRTADFLWMMIFG+ +LLVLS IP F  P LG+SLVFMLLY+WSREFPNA I++YGLVT
Sbjct: 121 ERRTADFLWMMIFGSFTLLVLSVIPFFWTPFLGVSLVFMLLYLWSREFPNANISLYGLVT 180

Query: 181 LKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWMYPSS 240
           LKAFYLPWAMLALDVIFGSP++PDLLGI+AGHLYYFLTVLHPLA GKN L+TP W+    
Sbjct: 181 LKAFYLPWAMLALDVIFGSPIMPDLLGIIAGHLYYFLTVLHPLATGKNYLKTPKWV---- 240

Query: 241 FVKFITNASPPTKQSYTLRQRLVSRWRIGAPPIQ-------RAAVPDDRGTSG------- 296
                               ++V+RWRIGAP           AA P   G  G       
Sbjct: 241 -------------------NKIVARWRIGAPVASVRQAGGVGAAGPGAGGGVGGGGAYSS 264

BLAST of Lsi06G002300 vs. TAIR 10
Match: AT4G04860.1 (DERLIN-2.2 )

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-27
Identity = 76/240 (31.67%), Postives = 117/240 (48.75%), Query Frame = 0

Query: 6   EFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSFYVKSC 65
           E+Y  +P I+++Y T  ++ T    L +  P  + L+  LV K +Q              
Sbjct: 7   EWYKQMPIITRSYLTAAVITTVGCSLDIISPYNLYLNPTLVVKQYQ-------------- 66

Query: 66  WKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPFQRRTA 125
                              WRL TNF +  K  ++F   +  +ARY   LE   F+ +TA
Sbjct: 67  ------------------YWRLVTNFLYFRKMDLDFMFHMFFLARYCKLLEENSFRGKTA 126

Query: 126 DFLWMMIFGALSL----LVLSAIPIFT-----FPVLGISLVFMLLYVWSREFPNAQINIY 185
           DFL+M++FGA  L    L+   IP  +        L  SL FM++YVWS++ P   ++  
Sbjct: 127 DFLYMLLFGASVLTGIVLIGGMIPYLSASFAKIIFLSNSLTFMMVYVWSKQNPYIHMSFL 186

Query: 186 GLVTLKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWM 237
           GL T  A YLPW +L   ++ G+    DLLG++AGH YYFL  ++P    +  L+TP ++
Sbjct: 187 GLFTFTAAYLPWVLLGFSILVGASAWVDLLGMIAGHAYYFLAEVYPRMTNRRPLKTPSFL 214

BLAST of Lsi06G002300 vs. TAIR 10
Match: AT4G21810.1 (DERLIN-2.1 )

HSP 1 Score: 119.4 bits (298), Expect = 4.9e-27
Identity = 75/240 (31.25%), Postives = 116/240 (48.33%), Query Frame = 0

Query: 6   EFYHSLPPISKAYGTLCLLATTAFQLGLYDPGLIALDYGLVFKHFQDVKCYTNSFYVKSC 65
           E+Y  +P I+++Y T  ++ T    L +  P  + L+  LV K +Q              
Sbjct: 7   EWYKQMPIITRSYLTAAVVTTVGCSLEIISPYNLYLNPTLVVKQYQ-------------- 66

Query: 66  WKALFQRFGQVKVVKECDVWRLFTNFFFLGKFSINFGIRLLMIARYGVQLENGPFQRRTA 125
                              WRL TNF +  K  ++F   +  +ARY   LE   F+ +TA
Sbjct: 67  ------------------FWRLVTNFLYFRKMDLDFLFHMFFLARYCKLLEENSFRGKTA 126

Query: 126 DFLWMMIFGALSL----LVLSAIPIFTFP-----VLGISLVFMLLYVWSREFPNAQINIY 185
           DFL+M++FGA  L    L+   IP  +        L  SL FM++YVWS++ P   ++  
Sbjct: 127 DFLYMLLFGATVLTGIVLIGGMIPYLSVSFSKIIFLSNSLTFMMVYVWSKQNPYIHMSFL 186

Query: 186 GLVTLKAFYLPWAMLALDVIFGSPLVPDLLGILAGHLYYFLTVLHPLAGGKNILRTPFWM 237
           GL T  A YLPW +L   ++ G+    D LG++AGH YYFL  ++P    +  L+TP ++
Sbjct: 187 GLFTFTAAYLPWVLLGFSILVGASAWGDFLGMIAGHAYYFLAFVYPRMTDRRPLKTPSFL 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZU93.0e-8557.05Derlin-1 OS=Arabidopsis thaliana OX=3702 GN=DER1 PE=2 SV=1[more]
Q063973.4e-8155.85Derlin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=DER1 PE=2 SV=2[more]
Q4G2J51.3e-7755.37Derlin-1.2 OS=Zea mays OX=4577 GN=DER1.2 PE=2 SV=1[more]
Q4G2J66.6e-7754.55Derlin-1.1 OS=Zea mays OX=4577 GN=DER1.1 PE=2 SV=2[more]
Q96Q809.0e-3435.92Derlin-3 OS=Homo sapiens OX=9606 GN=DERL3 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CDT15.9e-12178.79Derlin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002800 PE=3 SV... [more]
A0A1S3BQ275.9e-12178.79Derlin OS=Cucumis melo OX=3656 GN=LOC103492274 PE=3 SV=1[more]
A0A0A0LZA31.3e-12078.45Derlin OS=Cucumis sativus OX=3659 GN=Csa_1G575080 PE=3 SV=1[more]
A0A6J1HA501.0e-11778.04Derlin OS=Cucurbita moschata OX=3662 GN=LOC111461868 PE=3 SV=1[more]
A0A6J1JBH34.0e-11777.70Derlin OS=Cucurbita maxima OX=3661 GN=LOC111485268 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038878469.13.8e-12279.46derlin-1 [Benincasa hispida][more]
XP_008450797.11.2e-12078.79PREDICTED: derlin-1 [Cucumis melo] >TYK10137.1 derlin-1 [Cucumis melo var. makuw... [more]
XP_004135679.12.7e-12078.45derlin-1 [Cucumis sativus] >KGN66192.1 hypothetical protein Csa_007006 [Cucumis ... [more]
XP_022961351.12.2e-11778.04derlin-1-like [Cucurbita moschata] >XP_023516821.1 derlin-1-like isoform X1 [Cuc... [more]
KAG7023624.14.8e-11777.70Derlin-1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT4G29330.12.1e-8657.05DERLIN-1 [more]
AT4G04860.11.3e-2731.67DERLIN-2.2 [more]
AT4G21810.14.9e-2731.25DERLIN-2.1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007599DerlinPFAMPF04511DER1coord: 77..233
e-value: 5.2E-38
score: 130.9
NoneNo IPR availablePANTHERPTHR11009:SF32DERLIN-1coord: 1..63
coord: 77..296
NoneNo IPR availablePANTHERPTHR11009DER1-LIKE PROTEIN, DERLINcoord: 1..63
NoneNo IPR availablePANTHERPTHR11009DER1-LIKE PROTEIN, DERLINcoord: 77..296
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 10..218

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G002300.1Lsi06G002300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:1990381 ubiquitin-specific protease binding