Lsi06G001520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G001520
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr06: 1619695 .. 1621074 (+)
RNA-Seq ExpressionLsi06G001520
SyntenyLsi06G001520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATACAGAAATTGATCAAGGGATCAAGATTACTCGTTTTTCACCGAAATGTGTACTTAATACATTTCCAATTGCTTAGCACTTTCAGTCCAAACAAATCTGACTATGTGAGTCATCATAAACAACGTGAAAAGCTCCGTGAACACATTCCTTTTGTCACCGATGTTAAAGAGGTGAAGGACCCATGTGAGGCAATGGCTCTCTTCGAGGATTATCGTGAAAGGGGCTTCAAACACCACTATCCTTCCTACTCATCTCTCATTTACAAGTTGGCTCGTTCTCGTAGATTTGAAGCTGTTGAGACAATTCTTGGTCATTTACAAAGTAGGAACATTCGCTGTGGTGAGACCCTTTTCGTCGCTTTGATTCAACATTATGGGAAAGCCCATTTAGTTGAGAAAGGTATTGAGCTTTTCCATCGAATGCCTTCCTTTAATTGCTTTCGTACTCTACAGTCCTTGAATGTATTGCTTAATACACTAGTTGACTGTGACCAGCTTTCTAAAGCAAGTGAAATCTTCCAGCAGGCTTATGAAATGGGCTTCCGTCCTAATTCAGTGTCCTATAATATAATGATCAAGGGTTGGATCAAGAAGGGTGGATGGGAACAAGCATGTAACTTGTTTGACGAAATGCTTGAGAAGAGAGTGCAACCTAGTGTAGTAACATACAATAGTTTTTTAGGGGTTTTATGTAGAAATGGTGAAATGGACACAGCACTGTGTTTGTTTGAGAACATGACTCAGAAAGGACATCATCCAAATGCTATTACTTATGCATTGTTAATGGAAGGATGGTGCTTCATAGGTAAATACAAAGAAGCCAAAAAGTTGATGTTTGATATGGAGTTTCGTGGTTGTAAACTGCGGCCTGTGAACTACGGTGTTTTAATGACACATCTAGGAAAGACAGGGAACATCGACGAGATGGAATCTTTACTTAATGAGATGAAGAAAAGACGGTTGAAGCCTGATGTCGTGACTTACAATATTCTTGTAAATTACTTGTGCAAAGAAGGAAAGATTGGGGATGCTTATAAGGTTTTGATTAAAATGCAAGTTGGAGGTTGTGATCCTAATGCAGCAACTTACAGGATGATGATTGATGGATTTTGTAATGCAGGGGATTTTGATGGGGCTATGAAGGTTTTGAATGCCATGTTGATGAGTGGGCATTGTCCTCATTTGCAAACTTTTGTTTTTCTAGTTCTTGGCCTTTTGAAAGGTGAAAACAAGGATTATGTTTGCTTTGTATTGGAGGAGATGGAAAAGAGACAGCTGAGGTTTGATGCTGAAACTTGGAGGATTTTGGTTATGGATGCTTGTGGTCAACATGTTGAAATAGGTAACCACATAAATGACTTGATCTCCTTGCACTGA

mRNA sequence

ATGATACAGAAATTGATCAAGGGATCAAGATTACTCGTTTTTCACCGAAATGTGTACTTAATACATTTCCAATTGCTTAGCACTTTCAGTCCAAACAAATCTGACTATGTGAGTCATCATAAACAACGTGAAAAGCTCCGTGAACACATTCCTTTTGTCACCGATGTTAAAGAGGTGAAGGACCCATGTGAGGCAATGGCTCTCTTCGAGGATTATCGTGAAAGGGGCTTCAAACACCACTATCCTTCCTACTCATCTCTCATTTACAAGTTGGCTCGTTCTCGTAGATTTGAAGCTGTTGAGACAATTCTTGGTCATTTACAAAGTAGGAACATTCGCTGTGGTGAGACCCTTTTCGTCGCTTTGATTCAACATTATGGGAAAGCCCATTTAGTTGAGAAAGGTATTGAGCTTTTCCATCGAATGCCTTCCTTTAATTGCTTTCGTACTCTACAGTCCTTGAATGTATTGCTTAATACACTAGTTGACTGTGACCAGCTTTCTAAAGCAAGTGAAATCTTCCAGCAGGCTTATGAAATGGGCTTCCGTCCTAATTCAGTGTCCTATAATATAATGATCAAGGGTTGGATCAAGAAGGGTGGATGGGAACAAGCATGTAACTTGTTTGACGAAATGCTTGAGAAGAGAGTGCAACCTAGTGTAGTAACATACAATAGTTTTTTAGGGGTTTTATGTAGAAATGGTGAAATGGACACAGCACTGTGTTTGTTTGAGAACATGACTCAGAAAGGACATCATCCAAATGCTATTACTTATGCATTGTTAATGGAAGGATGGTGCTTCATAGGTAAATACAAAGAAGCCAAAAAGTTGATGTTTGATATGGAGTTTCGTGGTTGTAAACTGCGGCCTGTGAACTACGGTGTTTTAATGACACATCTAGGAAAGACAGGGAACATCGACGAGATGGAATCTTTACTTAATGAGATGAAGAAAAGACGGTTGAAGCCTGATGTCGTGACTTACAATATTCTTGTAAATTACTTGTGCAAAGAAGGAAAGATTGGGGATGCTTATAAGGTTTTGATTAAAATGCAAGTTGGAGGTTGTGATCCTAATGCAGCAACTTACAGGATGATGATTGATGGATTTTGTAATGCAGGGGATTTTGATGGGGCTATGAAGGTTTTGAATGCCATGTTGATGAGTGGGCATTGTCCTCATTTGCAAACTTTTGTTTTTCTAGTTCTTGGCCTTTTGAAAGGTGAAAACAAGGATTATGTTTGCTTTGTATTGGAGGAGATGGAAAAGAGACAGCTGAGGTTTGATGCTGAAACTTGGAGGATTTTGGTTATGGATGCTTGTGGTCAACATGTTGAAATAGGTAACCACATAAATGACTTGATCTCCTTGCACTGA

Coding sequence (CDS)

ATGATACAGAAATTGATCAAGGGATCAAGATTACTCGTTTTTCACCGAAATGTGTACTTAATACATTTCCAATTGCTTAGCACTTTCAGTCCAAACAAATCTGACTATGTGAGTCATCATAAACAACGTGAAAAGCTCCGTGAACACATTCCTTTTGTCACCGATGTTAAAGAGGTGAAGGACCCATGTGAGGCAATGGCTCTCTTCGAGGATTATCGTGAAAGGGGCTTCAAACACCACTATCCTTCCTACTCATCTCTCATTTACAAGTTGGCTCGTTCTCGTAGATTTGAAGCTGTTGAGACAATTCTTGGTCATTTACAAAGTAGGAACATTCGCTGTGGTGAGACCCTTTTCGTCGCTTTGATTCAACATTATGGGAAAGCCCATTTAGTTGAGAAAGGTATTGAGCTTTTCCATCGAATGCCTTCCTTTAATTGCTTTCGTACTCTACAGTCCTTGAATGTATTGCTTAATACACTAGTTGACTGTGACCAGCTTTCTAAAGCAAGTGAAATCTTCCAGCAGGCTTATGAAATGGGCTTCCGTCCTAATTCAGTGTCCTATAATATAATGATCAAGGGTTGGATCAAGAAGGGTGGATGGGAACAAGCATGTAACTTGTTTGACGAAATGCTTGAGAAGAGAGTGCAACCTAGTGTAGTAACATACAATAGTTTTTTAGGGGTTTTATGTAGAAATGGTGAAATGGACACAGCACTGTGTTTGTTTGAGAACATGACTCAGAAAGGACATCATCCAAATGCTATTACTTATGCATTGTTAATGGAAGGATGGTGCTTCATAGGTAAATACAAAGAAGCCAAAAAGTTGATGTTTGATATGGAGTTTCGTGGTTGTAAACTGCGGCCTGTGAACTACGGTGTTTTAATGACACATCTAGGAAAGACAGGGAACATCGACGAGATGGAATCTTTACTTAATGAGATGAAGAAAAGACGGTTGAAGCCTGATGTCGTGACTTACAATATTCTTGTAAATTACTTGTGCAAAGAAGGAAAGATTGGGGATGCTTATAAGGTTTTGATTAAAATGCAAGTTGGAGGTTGTGATCCTAATGCAGCAACTTACAGGATGATGATTGATGGATTTTGTAATGCAGGGGATTTTGATGGGGCTATGAAGGTTTTGAATGCCATGTTGATGAGTGGGCATTGTCCTCATTTGCAAACTTTTGTTTTTCTAGTTCTTGGCCTTTTGAAAGGTGAAAACAAGGATTATGTTTGCTTTGTATTGGAGGAGATGGAAAAGAGACAGCTGAGGTTTGATGCTGAAACTTGGAGGATTTTGGTTATGGATGCTTGTGGTCAACATGTTGAAATAGGTAACCACATAAATGACTTGATCTCCTTGCACTGA

Protein sequence

MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVKDPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFVALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEMGFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTALCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAETWRILVMDACGQHVEIGNHINDLISLH
Homology
BLAST of Lsi06G001520 vs. ExPASy Swiss-Prot
Match: Q9LQQ1 (Pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g07740 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.7e-140
Identity = 245/423 (57.92%), Postives = 307/423 (72.58%), Query Frame = 0

Query: 22  HFQLLSTFSPNK--SDYVSHHKQREKLREHIPFVTDVKEVKDPCEAMALFEDYRERGFKH 81
           H+       P K  S +   HK   K  E +PF+TD+KE++DP EA++LF  Y+E GF+H
Sbjct: 20  HYHTSRPEKPTKKASSHEPTHKFTRKPWEEVPFLTDLKEIEDPEEALSLFHQYQEMGFRH 79

Query: 82  HYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFVALIQHYGKAHLVEKGIELF 141
            YPSYSSLIYKLA+SR F+AV+ IL  ++ RN+RC E+LF+ LIQHYGKA  V+K I++F
Sbjct: 80  DYPSYSSLIYKLAKSRNFDAVDQILRLVRYRNVRCRESLFMGLIQHYGKAGSVDKAIDVF 139

Query: 142 HRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEMGFRPNSVSYNIMIKGWIKK 201
           H++ SF+C RT+QSLN L+N LVD  +L KA   F  A +M  RPNSVS+NI+IKG++ K
Sbjct: 140 HKITSFDCVRTIQSLNTLINVLVDNGELEKAKSFFDGAKDMRLRPNSVSFNILIKGFLDK 199

Query: 202 GGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTALCLFENMTQKGHHPNAITY 261
             WE AC +FDEMLE  VQPSVVTYNS +G LCRN +M  A  L E+M +K   PNA+T+
Sbjct: 200 CDWEAACKVFDEMLEMEVQPSVVTYNSLIGFLCRNDDMGKAKSLLEDMIKKRIRPNAVTF 259

Query: 262 ALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLGKTGNIDEMESLLNEMKK 321
            LLM+G C  G+Y EAKKLMFDME+RGCK   VNYG+LM+ LGK G IDE + LL EMKK
Sbjct: 260 GLLMKGLCCKGEYNEAKKLMFDMEYRGCKPGLVNYGILMSDLGKRGRIDEAKLLLGEMKK 319

Query: 322 RRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAATYRMMIDGFCNAGDFDG 381
           RR+KPDVV YNILVN+LC E ++ +AY+VL +MQ+ GC PNAATYRMMIDGFC   DFD 
Sbjct: 320 RRIKPDVVIYNILVNHLCTECRVPEAYRVLTEMQMKGCKPNAATYRMMIDGFCRIEDFDS 379

Query: 382 AMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAETWRILVM 441
            + VLNAML S HCP   TFV +V GL+KG N D+ CFVLE M K+ L F +  W+ L+ 
Sbjct: 380 GLNVLNAMLASRHCPTPATFVCMVAGLIKGGNLDHACFVLEVMGKKNLSFGSGAWQNLLS 439

Query: 442 DAC 443
           D C
Sbjct: 440 DLC 442

BLAST of Lsi06G001520 vs. ExPASy Swiss-Prot
Match: Q9FFE3 (Pentatricopeptide repeat-containing protein At5g16420, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g16420 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.1e-52
Identity = 114/366 (31.15%), Postives = 200/366 (54.64%), Query Frame = 0

Query: 76  GFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSR--NIRCGETLFVALIQHYGKAHLVE 135
           GF H+Y +Y S+++KL+R+R F+ VE+++  L++    I+CGE LF+ L+++YG A   E
Sbjct: 78  GFTHNYDTYHSILFKLSRARAFDPVESLMADLRNSYPPIKCGENLFIDLLRNYGLAGRYE 137

Query: 136 KGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYE-MGFRPNSVSYNIM 195
             + +F R+P F   R+++SLN LLN L+   +      +F+ + E  G  PN  + N++
Sbjct: 138 SSMRIFLRIPDFGVKRSVRSLNTLLNVLIQNQRFDLVHAMFKNSKESFGITPNIFTCNLL 197

Query: 196 IKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTALCLFENMTQKGH 255
           +K   KK   E A  + DE+    + P++VTY + LG     G+M++A  + E M  +G 
Sbjct: 198 VKALCKKNDIESAYKVLDEIPSMGLVPNLVTYTTILGGYVARGDMESAKRVLEEMLDRGW 257

Query: 256 HPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLGKTGNIDEMES 315
           +P+A TY +LM+G+C +G++ EA  +M DME    +   V YGV++  L K     E  +
Sbjct: 258 YPDATTYTVLMDGYCKLGRFSEAATVMDDMEKNEIEPNEVTYGVMIRALCKEKKSGEARN 317

Query: 316 LLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAATYRMMIDGFC 375
           + +EM +R   PD      +++ LC++ K+ +A  +  KM    C P+ A    +I   C
Sbjct: 318 MFDEMLERSFMPDSSLCCKVIDALCEDHKVDEACGLWRKMLKNNCMPDNALLSTLIHWLC 377

Query: 376 NAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAE 435
             G    A K+ +     G  P L T+  L+ G+ +         + ++M +R+ + +A 
Sbjct: 378 KEGRVTEARKLFDE-FEKGSIPSLLTYNTLIAGMCEKGELTEAGRLWDDMYERKCKPNAF 437

Query: 436 TWRILV 439
           T+ +L+
Sbjct: 438 TYNVLI 442

BLAST of Lsi06G001520 vs. ExPASy Swiss-Prot
Match: Q9MAG8 (Putative pentatricopeptide repeat-containing protein At1g53330 OS=Arabidopsis thaliana OX=3702 GN=At1g53330 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.9e-48
Identity = 128/446 (28.70%), Postives = 215/446 (48.21%), Query Frame = 0

Query: 56  VKEVKDPCEAMALFED------YRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQS 115
           ++   DP  AM LF +        +R F++    Y  +I KL  S+ F+ ++ +L HL++
Sbjct: 16  LRRENDPSAAMKLFRNPDPESTNPKRPFRYSLLCYDIIITKLGGSKMFDELDQVLLHLKT 75

Query: 116 -RNIRCGETLFVALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLS 175
              I   E +F  +I  +G+  L  + + +F  MP + C RT++SLN LL+ L+ C +L 
Sbjct: 76  DTRIVPTEIIFCNVINFFGRGKLPSRALHMFDEMPQYRCQRTVKSLNSLLSALLKCGELE 135

Query: 176 KASEIFQQAYEMGFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFL 235
           K  E      E G +P++ +YNI+I G  + G ++ A  LFDEM++K+V+P+ VT+ + +
Sbjct: 136 KMKERLSSIDEFG-KPDACTYNILIHGCSQSGCFDDALKLFDEMVKKKVKPTGVTFGTLI 195

Query: 236 GVLCRNGEMDTALCLFENMTQ-KGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGC 295
             LC++  +  AL +  +M +  G  P    YA L++  C IG+   A KL  +      
Sbjct: 196 HGLCKDSRVKEALKMKHDMLKVYGVRPTVHIYASLIKALCQIGELSFAFKLKDEAYEGKI 255

Query: 296 KLRPVNYGVLMTHLGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYK 355
           K+    Y  L++ L K G  +E+  +L EM ++  KPD VTYN+L+N  C E     A +
Sbjct: 256 KVDAAIYSTLISSLIKAGRSNEVSMILEEMSEKGCKPDTVTYNVLINGFCVENDSESANR 315

Query: 356 VLIKM-QVG----------------------------------GCDPNAATYRMMIDGFC 415
           VL +M + G                                  GC P+  +YR++ DG C
Sbjct: 316 VLDEMVEKGLKPDVISYNMILGVFFRIKKWEEATYLFEDMPRRGCSPDTLSYRIVFDGLC 375

Query: 416 NAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAE 459
               F+ A  +L+ ML  G+ P        +  L +    + +  V+  +  R +  DA+
Sbjct: 376 EGLQFEEAAVILDEMLFKGYKPRRDRLEGFLQKLCESGKLEILSKVISSLH-RGIAGDAD 435

BLAST of Lsi06G001520 vs. ExPASy Swiss-Prot
Match: Q3E9F0 (Pentatricopeptide repeat-containing protein At5g18475 OS=Arabidopsis thaliana OX=3702 GN=At5g18475 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.4e-46
Identity = 123/457 (26.91%), Postives = 227/457 (49.67%), Query Frame = 0

Query: 43  REKLREHIPFVTDVKEVKDPCEAMALF-EDYRERGFKHHYPSYSSLIYKLARSRRFEAVE 102
           + K   H   V+ +K  +DP   + +F +  +++GF H+  +YS L+  L R ++F AV+
Sbjct: 50  KTKFISHESAVSLMKRERDPQGVLDIFNKASQQKGFNHNNATYSVLLDNLVRHKKFLAVD 109

Query: 103 TILGHLQSRNIRCGETLFVALIQHYGKAHLVEKGIELFHRMPSFNCFR-TLQSLNVLLNT 162
            IL  ++    R  E+LF+ L++H+ ++ L +K +E+F+ +      + +L +++  LN 
Sbjct: 110 AILHQMKYETCRFQESLFLNLMRHFSRSDLHDKVMEMFNLIQVIARVKPSLNAISTCLNL 169

Query: 163 LVDCDQLSKASEIFQQA-YEMGFRPNSVSYNIMIKGWIKKGG------------------ 222
           L+D  +++ + ++   A + +G +PN+  +NI++K   K G                   
Sbjct: 170 LIDSGEVNLSRKLLLYAKHNLGLQPNTCIFNILVKHHCKNGDINFAFLVVEEMKRSGISY 229

Query: 223 ------------------WEQACNLFDEMLEKR-VQPSVVTYNSFLGVLCRNGEMDTALC 282
                              ++A  LF++M+ K  + P  VT+N  +   CR GE++ A  
Sbjct: 230 PNSITYSTLMDCLFAHSRSKEAVELFEDMISKEGISPDPVTFNVMINGFCRAGEVERAKK 289

Query: 283 LFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLG 342
           + + M + G +PN   Y+ LM G+C +GK +EAK+   +++  G KL  V Y  LM    
Sbjct: 290 ILDFMKKNGCNPNVYNYSALMNGFCKVGKIQEAKQTFDEVKKTGLKLDTVGYTTLMNCFC 349

Query: 343 KTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAA 402
           + G  DE   LL EMK  R + D +TYN+++  L  EG+  +A ++L +    G   N  
Sbjct: 350 RNGETDEAMKLLGEMKASRCRADTLTYNVILRGLSSEGRSEEALQMLDQWGSEGVHLNKG 409

Query: 403 TYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEM 458
           +YR++++  C  G+ + A+K L+ M   G  PH  T+  LV+ L +    +    VL   
Sbjct: 410 SYRIILNALCCNGELEKAVKFLSVMSERGIWPHHATWNELVVRLCESGYTEIGVRVLIGF 469

BLAST of Lsi06G001520 vs. ExPASy Swiss-Prot
Match: O49436 (Pentatricopeptide repeat-containing protein At4g20090 OS=Arabidopsis thaliana OX=3702 GN=EMB1025 PE=3 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.2e-46
Identity = 126/429 (29.37%), Postives = 202/429 (47.09%), Query Frame = 0

Query: 59  VKDPCEA---MALFEDYRERG-FKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRC 118
           V++P EA     +F+   + G FK    + SS+I   A S  F++VE +L  ++  N   
Sbjct: 51  VENPLEAPISEKMFKSAPKMGSFKLGDSTLSSMIESYANSGDFDSVEKLLSRIRLENRVI 110

Query: 119 GETLFVALIQHYGKAHLVEKGIELFHRM-PSFNCFRTLQSLNVLLNTLVDCDQLSKASEI 178
            E  F+ + + YGKAHL +K ++LFHRM   F C R+++S N +LN +++     +  E 
Sbjct: 111 IERSFIVVFRAYGKAHLPDKAVDLFHRMVDEFRCKRSVKSFNSVLNVIINEGLYHRGLEF 170

Query: 179 FQQA----YEMGFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLG 238
           +         M   PN +S+N++IK   K    ++A  +F  M E++  P   TY + + 
Sbjct: 171 YDYVVNSNMNMNISPNGLSFNLVIKALCKLRFVDRAIEVFRGMPERKCLPDGYTYCTLMD 230

Query: 239 VLCRNGEMDTALCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKL 298
            LC+   +D A+ L + M  +G  P+ + Y +L++G C  G      KL+ +M  +GC  
Sbjct: 231 GLCKEERIDEAVLLLDEMQSEGCSPSPVIYNVLIDGLCKKGDLTRVTKLVDNMFLKGCVP 290

Query: 299 RPVNYGVLMTHLGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDA---- 358
             V Y  L+  L   G +D+  SLL  M   +  P+ VTY  L+N L K+ +  DA    
Sbjct: 291 NEVTYNTLIHGLCLKGKLDKAVSLLERMVSSKCIPNDVTYGTLINGLVKQRRATDAVRLL 350

Query: 359 --------------YKVLI-----------------KMQVGGCDPNAATYRMMIDGFCNA 418
                         Y VLI                 KM   GC PN   Y +++DG C  
Sbjct: 351 SSMEERGYHLNQHIYSVLISGLFKEGKAEEAMSLWRKMAEKGCKPNIVVYSVLVDGLCRE 410

Query: 419 GDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAETW 444
           G  + A ++LN M+ SG  P+  T+  L+ G  K    +    V +EM+K     +   +
Sbjct: 411 GKPNEAKEILNRMIASGCLPNAYTYSSLMKGFFKTGLCEEAVQVWKEMDKTGCSRNKFCY 470

BLAST of Lsi06G001520 vs. ExPASy TrEMBL
Match: A0A0A0M1K9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570750 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.5e-248
Identity = 415/459 (90.41%), Postives = 438/459 (95.42%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIK SRLL+FHRNV LI FQLLSTFSPNK D+ ++HKQR K+RE IPFVTDVKEVK
Sbjct: 1   MIQKLIKRSRLLIFHRNVCLIRFQLLSTFSPNKFDFENNHKQRRKIRERIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFE+Y E+GFKHHYPSYSSLIYKLARSRRFEAVETILGHL++RNIRC ETLFV
Sbjct: 61  DPCEALALFENYHEKGFKHHYPSYSSLIYKLARSRRFEAVETILGHLRNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFH+MPSFNCFRTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHQMPSFNCFRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
            FRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEK VQPSVVTYNSFLGVLCR GEMDTA
Sbjct: 181 DFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKGVQPSVVTYNSFLGVLCRKGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLF+NMT+KGHHPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCKLRPVNYGVLMTH
Sbjct: 241 LCLFKNMTEKGHHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKLRPVNYGVLMTH 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGK+GDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKVGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENK-DYVCFVL 420
           AATYRMMIDG+CNAGDFDGAMK+LNAMLMSGH PHL+TF  LV+GLLKGEN  DYVCFVL
Sbjct: 361 AATYRMMIDGYCNAGDFDGAMKILNAMLMSGHYPHLKTFASLVVGLLKGENNDDYVCFVL 420

Query: 421 EEMEKRQLRFDAETWRILVMDACGQHVEIGNHINDLISL 459
           EEMEKRQLRFDAETWRIL+MD CGQHVEIG+H+NDLISL
Sbjct: 421 EEMEKRQLRFDAETWRILIMDVCGQHVEIGSHLNDLISL 459

BLAST of Lsi06G001520 vs. ExPASy TrEMBL
Match: A0A1S4DYZ6 (pentatricopeptide repeat-containing protein At1g07740, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492193 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.2e-244
Identity = 408/450 (90.67%), Postives = 428/450 (95.11%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIK S LL+FHRNV LIHFQLLSTFS NK D  +HHKQR KLRE IPFVTDVKEVK
Sbjct: 1   MIQKLIKRSSLLIFHRNVCLIHFQLLSTFSTNKFDSENHHKQRRKLREPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFE+Y E+GFKHHYPSYSSLIYKLARSRRFEAVETILGHL++RNIRC ETLFV
Sbjct: 61  DPCEALALFENYHEKGFKHHYPSYSSLIYKLARSRRFEAVETILGHLRNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFH+MPSFNCFRTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHQMPSFNCFRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEK VQPSVVTYNSFLGVLCR GEMDTA
Sbjct: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKGVQPSVVTYNSFLGVLCRKGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLF+NMT+KGHHPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCKLRPVNYGVLMT+
Sbjct: 241 LCLFKNMTEKGHHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKLRPVNYGVLMTY 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGK GNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEG++GDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKAGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENK-DYVCFVL 420
           AATYRMMIDG+CNAGDFDGAMK+LNAMLMSGHCPHL+TF FLV+GLLKGEN  DYVCFVL
Sbjct: 361 AATYRMMIDGYCNAGDFDGAMKILNAMLMSGHCPHLKTFAFLVVGLLKGENNDDYVCFVL 420

Query: 421 EEMEKRQLRFDAETWRILVMDACGQHVEIG 450
           EEMEKRQLRFDAETWR L+MD CGQHVEIG
Sbjct: 421 EEMEKRQLRFDAETWRTLIMDVCGQHVEIG 450

BLAST of Lsi06G001520 vs. ExPASy TrEMBL
Match: A0A5D3CEM1 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003670 PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.5e-244
Identity = 407/450 (90.44%), Postives = 427/450 (94.89%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIK S LL+FHRNV LIHFQLLSTFS NK D  +HHKQR KL E IPFVTDVKEVK
Sbjct: 1   MIQKLIKRSSLLIFHRNVCLIHFQLLSTFSTNKFDSENHHKQRRKLHEPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFE+Y E+GFKHHYPSYSSLIYKLARSRRFEAVETILGHL++RNIRC ETLFV
Sbjct: 61  DPCEALALFENYHEKGFKHHYPSYSSLIYKLARSRRFEAVETILGHLRNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFH+MPSFNCFRTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHQMPSFNCFRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEK VQPSVVTYNSFLGVLCR GEMDTA
Sbjct: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKGVQPSVVTYNSFLGVLCRKGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLF+NMT+KGHHPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCKLRPVNYGVLMT+
Sbjct: 241 LCLFKNMTEKGHHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKLRPVNYGVLMTY 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGK GNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEG++GDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKAGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENK-DYVCFVL 420
           AATYRMMIDG+CNAGDFDGAMK+LNAMLMSGHCPHL+TF FLV+GLLKGEN  DYVCFVL
Sbjct: 361 AATYRMMIDGYCNAGDFDGAMKILNAMLMSGHCPHLKTFAFLVVGLLKGENNDDYVCFVL 420

Query: 421 EEMEKRQLRFDAETWRILVMDACGQHVEIG 450
           EEMEKRQLRFDAETWR L+MD CGQHVEIG
Sbjct: 421 EEMEKRQLRFDAETWRTLIMDVCGQHVEIG 450

BLAST of Lsi06G001520 vs. ExPASy TrEMBL
Match: A0A6J1HAJ5 (pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462115 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.6e-240
Identity = 406/459 (88.45%), Postives = 431/459 (93.90%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIKGSRLLVFHRNV LIHFQLLST  PNK D+VS HKQ +KLRE IPFVTDVKEVK
Sbjct: 1   MIQKLIKGSRLLVFHRNVCLIHFQLLSTHRPNKFDHVSPHKQHQKLREPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALF+DY ERGFKHHYPSYSSLIYKLARSRRFEAVE ILGHLQ+RNIRC ETLFV
Sbjct: 61  DPCEALALFKDYHERGFKHHYPSYSSLIYKLARSRRFEAVEMILGHLQNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGK HLVEKGIELFHRMPSFNCFR+ QSLNVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKVHLVEKGIELFHRMPSFNCFRSRQSLNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSV+YNIMIKGWIKKG WEQACNLF+EMLEKRVQPSVVTYNS LG+LCRNGEMDTA
Sbjct: 181 GFRPNSVTYNIMIKGWIKKGEWEQACNLFNEMLEKRVQPSVVTYNSLLGLLCRNGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLFENM QKG+HPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCK RPVNYGVLMTH
Sbjct: 241 LCLFENMIQKGNHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKPRPVNYGVLMTH 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGKTGNIDE+ESLLNEMKKRRLKPDVVTYNILVNYLCKEG++ DAYKVL+KMQVGGC+PN
Sbjct: 301 LGKTGNIDEVESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVEDAYKVLVKMQVGGCNPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLE 420
           AATYRMMIDGFCNAGDFDGAMKVLN+MLMSGHCP LQTF  L++GLL+GEN +YV FVLE
Sbjct: 361 AATYRMMIDGFCNAGDFDGAMKVLNSMLMSGHCPRLQTFASLIVGLLRGENSNYVFFVLE 420

Query: 421 EMEKRQLRFDAETWRILVMDACGQH-VEIGNHINDLISL 459
           EMEKRQ+RFDAETWR LVMDACGQ  V+IG+ IN+LISL
Sbjct: 421 EMEKRQMRFDAETWRTLVMDACGQDVVDIGSRINELISL 459

BLAST of Lsi06G001520 vs. ExPASy TrEMBL
Match: A0A6J1HYX7 (pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467870 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.4e-240
Identity = 402/457 (87.96%), Postives = 427/457 (93.44%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           M QKLIKGSRLLVFHRNV LIHFQLLST  PN+ D VS HKQ +KLRE IPFVTDVKEVK
Sbjct: 1   MTQKLIKGSRLLVFHRNVCLIHFQLLSTHRPNEFDRVSPHKQHQKLREPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFEDY ERGFKHHYPSYSSLIYKLARSRRFEAVE IL HLQ+RNI C ETLFV
Sbjct: 61  DPCEALALFEDYHERGFKHHYPSYSSLIYKLARSRRFEAVEMILDHLQNRNIHCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVE+GI+LFHRMPSFNCFR+ QSLNVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVERGIDLFHRMPSFNCFRSRQSLNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSV+YNIMIKGWIKKG WEQACNLF+EMLEKRV+PSVVTYNS LG+LCRNGEMDTA
Sbjct: 181 GFRPNSVTYNIMIKGWIKKGEWEQACNLFNEMLEKRVRPSVVTYNSLLGLLCRNGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLFENM QKGHHPNA+TYALLMEGWC  GKYKEAKKLMFDMEF GCK RPVNYGVLMTH
Sbjct: 241 LCLFENMIQKGHHPNAVTYALLMEGWCLTGKYKEAKKLMFDMEFHGCKPRPVNYGVLMTH 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGKTGNIDE+ESLLNEMKKRRLKPDVVTYNILVNYLCKEG++GDAYKVL+KMQVGGC+PN
Sbjct: 301 LGKTGNIDEVESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVGDAYKVLVKMQVGGCNPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLE 420
           AATYRMMIDGFCNAGDFDGAMKVLN+MLMSGHCP LQTF  L++GLL+GEN DYVCFVLE
Sbjct: 361 AATYRMMIDGFCNAGDFDGAMKVLNSMLMSGHCPRLQTFASLIVGLLRGENSDYVCFVLE 420

Query: 421 EMEKRQLRFDAETWRILVMDACGQHVEIGNHINDLIS 458
           EMEKRQ+RFDAETWR LVMDACGQ VEIG+ I++LIS
Sbjct: 421 EMEKRQMRFDAETWRTLVMDACGQDVEIGSRISELIS 457

BLAST of Lsi06G001520 vs. NCBI nr
Match: XP_038878835.1 (pentatricopeptide repeat-containing protein At1g07740, mitochondrial isoform X1 [Benincasa hispida] >XP_038878836.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial isoform X1 [Benincasa hispida] >XP_038878837.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial isoform X1 [Benincasa hispida] >XP_038878838.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 882.5 bits (2279), Expect = 1.6e-252
Identity = 420/457 (91.90%), Postives = 440/457 (96.28%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIKGSRLLVFH+NV LIHFQLLST SPNKSDY++ HKQR+KLREHIPFVTDVKEVK
Sbjct: 1   MIQKLIKGSRLLVFHQNVCLIHFQLLSTLSPNKSDYMNRHKQRQKLREHIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DP EA+ALFEDY E+GFKHHYPSYS+L+YKLARSRRFE VETILGHLQ+RNIRC ETLFV
Sbjct: 61  DPSEALALFEDYHEKGFKHHYPSYSALLYKLARSRRFEVVETILGHLQNRNIRCSETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFHRMPSFNC RTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCIRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSVSYNIMIKGWIKKGGWEQACNLF+EMLEKRVQPSVVTYNSFLGVLCRNGEMDTA
Sbjct: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFNEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           L LF NMTQKGHHPNA+TYALLMEGWC+I KYKEAKKLMFDMEFRGCK RPVNYGVLMTH
Sbjct: 241 LSLFGNMTQKGHHPNAVTYALLMEGWCYICKYKEAKKLMFDMEFRGCKPRPVNYGVLMTH 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGKTGNIDEMESLLNEMKKRR+KPDVV YNILVNY+CKEG+IGDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKTGNIDEMESLLNEMKKRRMKPDVVMYNILVNYMCKEGRIGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLE 420
           AATYRMMIDGFCNAGDFDGAMK+LNAML SGHCPHL+TFVFLV+GLLKGEN DYVCFVLE
Sbjct: 361 AATYRMMIDGFCNAGDFDGAMKILNAMLTSGHCPHLKTFVFLVVGLLKGENNDYVCFVLE 420

Query: 421 EMEKRQLRFDAETWRILVMDACGQHVEIGNHINDLIS 458
           EMEKRQLRFDAETWRIL+MDACGQHVEIGNHINDLIS
Sbjct: 421 EMEKRQLRFDAETWRILIMDACGQHVEIGNHINDLIS 457

BLAST of Lsi06G001520 vs. NCBI nr
Match: XP_011659903.1 (pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucumis sativus] >KGN66101.1 hypothetical protein Csa_007130 [Cucumis sativus])

HSP 1 Score: 868.2 bits (2242), Expect = 3.1e-248
Identity = 415/459 (90.41%), Postives = 438/459 (95.42%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIK SRLL+FHRNV LI FQLLSTFSPNK D+ ++HKQR K+RE IPFVTDVKEVK
Sbjct: 1   MIQKLIKRSRLLIFHRNVCLIRFQLLSTFSPNKFDFENNHKQRRKIRERIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFE+Y E+GFKHHYPSYSSLIYKLARSRRFEAVETILGHL++RNIRC ETLFV
Sbjct: 61  DPCEALALFENYHEKGFKHHYPSYSSLIYKLARSRRFEAVETILGHLRNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFH+MPSFNCFRTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHQMPSFNCFRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
            FRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEK VQPSVVTYNSFLGVLCR GEMDTA
Sbjct: 181 DFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKGVQPSVVTYNSFLGVLCRKGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLF+NMT+KGHHPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCKLRPVNYGVLMTH
Sbjct: 241 LCLFKNMTEKGHHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKLRPVNYGVLMTH 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGK+GDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKVGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENK-DYVCFVL 420
           AATYRMMIDG+CNAGDFDGAMK+LNAMLMSGH PHL+TF  LV+GLLKGEN  DYVCFVL
Sbjct: 361 AATYRMMIDGYCNAGDFDGAMKILNAMLMSGHYPHLKTFASLVVGLLKGENNDDYVCFVL 420

Query: 421 EEMEKRQLRFDAETWRILVMDACGQHVEIGNHINDLISL 459
           EEMEKRQLRFDAETWRIL+MD CGQHVEIG+H+NDLISL
Sbjct: 421 EEMEKRQLRFDAETWRILIMDVCGQHVEIGSHLNDLISL 459

BLAST of Lsi06G001520 vs. NCBI nr
Match: XP_023520779.1 (pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023520780.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023520781.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023520782.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023520783.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023520784.1 pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.1 bits (2213), Expect = 7.1e-245
Identity = 410/458 (89.52%), Postives = 435/458 (94.98%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIKGSRLLVFHRNV L+HFQLLST  PNKSDYVS HKQ +KLRE IPFVTDVKEVK
Sbjct: 1   MIQKLIKGSRLLVFHRNVCLLHFQLLSTHRPNKSDYVSPHKQHQKLREPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFEDY ERGFKHHYPSYSSLIYKLARSRRFEAVE ILGHLQ+RNIRC ETLFV
Sbjct: 61  DPCEALALFEDYHERGFKHHYPSYSSLIYKLARSRRFEAVEMILGHLQNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFHRMPSFNCFR+ QSLNVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRSRQSLNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSV+YNIMIKGWIKKG WEQACNLF+EMLEKRVQPSVVTYNS LG+LCRNGEMDTA
Sbjct: 181 GFRPNSVTYNIMIKGWIKKGEWEQACNLFNEMLEKRVQPSVVTYNSLLGLLCRNGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLFENM QKG+HPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCK RPVNYGVLMTH
Sbjct: 241 LCLFENMIQKGNHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKPRPVNYGVLMTH 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGKTGNIDE+ESLLNEMKKRRLKPDVVTYNILVNYLCKEG++ DAYKVL+KMQVGGC+PN
Sbjct: 301 LGKTGNIDEVESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVEDAYKVLVKMQVGGCNPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLE 420
           AATYRMMIDGFCNAGDFDGAMKVLN+MLMSGHCP LQTF  L++GLL+GEN+DYVCFVLE
Sbjct: 361 AATYRMMIDGFCNAGDFDGAMKVLNSMLMSGHCPRLQTFASLIVGLLRGENRDYVCFVLE 420

Query: 421 EMEKRQLRFDAETWRILVMDACGQHVEIGNHINDLISL 459
           EMEKRQ+RFDAETWR LVMDACGQ V+IG+ I++LISL
Sbjct: 421 EMEKRQMRFDAETWRTLVMDACGQDVDIGSRISELISL 458

BLAST of Lsi06G001520 vs. NCBI nr
Match: XP_016900945.1 (PREDICTED: pentatricopeptide repeat-containing protein At1g07740, mitochondrial-like isoform X1 [Cucumis melo] >XP_016900946.1 PREDICTED: pentatricopeptide repeat-containing protein At1g07740, mitochondrial-like isoform X1 [Cucumis melo])

HSP 1 Score: 854.4 bits (2206), Expect = 4.6e-244
Identity = 408/450 (90.67%), Postives = 428/450 (95.11%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIK S LL+FHRNV LIHFQLLSTFS NK D  +HHKQR KLRE IPFVTDVKEVK
Sbjct: 1   MIQKLIKRSSLLIFHRNVCLIHFQLLSTFSTNKFDSENHHKQRRKLREPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFE+Y E+GFKHHYPSYSSLIYKLARSRRFEAVETILGHL++RNIRC ETLFV
Sbjct: 61  DPCEALALFENYHEKGFKHHYPSYSSLIYKLARSRRFEAVETILGHLRNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFH+MPSFNCFRTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHQMPSFNCFRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEK VQPSVVTYNSFLGVLCR GEMDTA
Sbjct: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKGVQPSVVTYNSFLGVLCRKGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLF+NMT+KGHHPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCKLRPVNYGVLMT+
Sbjct: 241 LCLFKNMTEKGHHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKLRPVNYGVLMTY 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGK GNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEG++GDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKAGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENK-DYVCFVL 420
           AATYRMMIDG+CNAGDFDGAMK+LNAMLMSGHCPHL+TF FLV+GLLKGEN  DYVCFVL
Sbjct: 361 AATYRMMIDGYCNAGDFDGAMKILNAMLMSGHCPHLKTFAFLVVGLLKGENNDDYVCFVL 420

Query: 421 EEMEKRQLRFDAETWRILVMDACGQHVEIG 450
           EEMEKRQLRFDAETWR L+MD CGQHVEIG
Sbjct: 421 EEMEKRQLRFDAETWRTLIMDVCGQHVEIG 450

BLAST of Lsi06G001520 vs. NCBI nr
Match: TYK10221.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 852.4 bits (2201), Expect = 1.8e-243
Identity = 407/450 (90.44%), Postives = 427/450 (94.89%), Query Frame = 0

Query: 1   MIQKLIKGSRLLVFHRNVYLIHFQLLSTFSPNKSDYVSHHKQREKLREHIPFVTDVKEVK 60
           MIQKLIK S LL+FHRNV LIHFQLLSTFS NK D  +HHKQR KL E IPFVTDVKEVK
Sbjct: 1   MIQKLIKRSSLLIFHRNVCLIHFQLLSTFSTNKFDSENHHKQRRKLHEPIPFVTDVKEVK 60

Query: 61  DPCEAMALFEDYRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFV 120
           DPCEA+ALFE+Y E+GFKHHYPSYSSLIYKLARSRRFEAVETILGHL++RNIRC ETLFV
Sbjct: 61  DPCEALALFENYHEKGFKHHYPSYSSLIYKLARSRRFEAVETILGHLRNRNIRCNETLFV 120

Query: 121 ALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEM 180
           ALIQHYGKAHLVEKGIELFH+MPSFNCFRTLQS NVLLNTLVDCDQ SKASEIFQQAYEM
Sbjct: 121 ALIQHYGKAHLVEKGIELFHQMPSFNCFRTLQSFNVLLNTLVDCDQFSKASEIFQQAYEM 180

Query: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTA 240
           GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEK VQPSVVTYNSFLGVLCR GEMDTA
Sbjct: 181 GFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKGVQPSVVTYNSFLGVLCRKGEMDTA 240

Query: 241 LCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTH 300
           LCLF+NMT+KGHHPNA+TYALLMEGWCFIGKYKEAKKLMFDMEF GCKLRPVNYGVLMT+
Sbjct: 241 LCLFKNMTEKGHHPNAVTYALLMEGWCFIGKYKEAKKLMFDMEFHGCKLRPVNYGVLMTY 300

Query: 301 LGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPN 360
           LGK GNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEG++GDAYKVL+KMQVGGCDPN
Sbjct: 301 LGKAGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGRVGDAYKVLVKMQVGGCDPN 360

Query: 361 AATYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENK-DYVCFVL 420
           AATYRMMIDG+CNAGDFDGAMK+LNAMLMSGHCPHL+TF FLV+GLLKGEN  DYVCFVL
Sbjct: 361 AATYRMMIDGYCNAGDFDGAMKILNAMLMSGHCPHLKTFAFLVVGLLKGENNDDYVCFVL 420

Query: 421 EEMEKRQLRFDAETWRILVMDACGQHVEIG 450
           EEMEKRQLRFDAETWR L+MD CGQHVEIG
Sbjct: 421 EEMEKRQLRFDAETWRTLIMDVCGQHVEIG 450

BLAST of Lsi06G001520 vs. TAIR 10
Match: AT1G07740.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.2e-141
Identity = 245/423 (57.92%), Postives = 307/423 (72.58%), Query Frame = 0

Query: 22  HFQLLSTFSPNK--SDYVSHHKQREKLREHIPFVTDVKEVKDPCEAMALFEDYRERGFKH 81
           H+       P K  S +   HK   K  E +PF+TD+KE++DP EA++LF  Y+E GF+H
Sbjct: 20  HYHTSRPEKPTKKASSHEPTHKFTRKPWEEVPFLTDLKEIEDPEEALSLFHQYQEMGFRH 79

Query: 82  HYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRCGETLFVALIQHYGKAHLVEKGIELF 141
            YPSYSSLIYKLA+SR F+AV+ IL  ++ RN+RC E+LF+ LIQHYGKA  V+K I++F
Sbjct: 80  DYPSYSSLIYKLAKSRNFDAVDQILRLVRYRNVRCRESLFMGLIQHYGKAGSVDKAIDVF 139

Query: 142 HRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYEMGFRPNSVSYNIMIKGWIKK 201
           H++ SF+C RT+QSLN L+N LVD  +L KA   F  A +M  RPNSVS+NI+IKG++ K
Sbjct: 140 HKITSFDCVRTIQSLNTLINVLVDNGELEKAKSFFDGAKDMRLRPNSVSFNILIKGFLDK 199

Query: 202 GGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTALCLFENMTQKGHHPNAITY 261
             WE AC +FDEMLE  VQPSVVTYNS +G LCRN +M  A  L E+M +K   PNA+T+
Sbjct: 200 CDWEAACKVFDEMLEMEVQPSVVTYNSLIGFLCRNDDMGKAKSLLEDMIKKRIRPNAVTF 259

Query: 262 ALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLGKTGNIDEMESLLNEMKK 321
            LLM+G C  G+Y EAKKLMFDME+RGCK   VNYG+LM+ LGK G IDE + LL EMKK
Sbjct: 260 GLLMKGLCCKGEYNEAKKLMFDMEYRGCKPGLVNYGILMSDLGKRGRIDEAKLLLGEMKK 319

Query: 322 RRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAATYRMMIDGFCNAGDFDG 381
           RR+KPDVV YNILVN+LC E ++ +AY+VL +MQ+ GC PNAATYRMMIDGFC   DFD 
Sbjct: 320 RRIKPDVVIYNILVNHLCTECRVPEAYRVLTEMQMKGCKPNAATYRMMIDGFCRIEDFDS 379

Query: 382 AMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAETWRILVM 441
            + VLNAML S HCP   TFV +V GL+KG N D+ CFVLE M K+ L F +  W+ L+ 
Sbjct: 380 GLNVLNAMLASRHCPTPATFVCMVAGLIKGGNLDHACFVLEVMGKKNLSFGSGAWQNLLS 439

Query: 442 DAC 443
           D C
Sbjct: 440 DLC 442

BLAST of Lsi06G001520 vs. TAIR 10
Match: AT5G16420.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 3.6e-53
Identity = 114/366 (31.15%), Postives = 200/366 (54.64%), Query Frame = 0

Query: 76  GFKHHYPSYSSLIYKLARSRRFEAVETILGHLQSR--NIRCGETLFVALIQHYGKAHLVE 135
           GF H+Y +Y S+++KL+R+R F+ VE+++  L++    I+CGE LF+ L+++YG A   E
Sbjct: 78  GFTHNYDTYHSILFKLSRARAFDPVESLMADLRNSYPPIKCGENLFIDLLRNYGLAGRYE 137

Query: 136 KGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLSKASEIFQQAYE-MGFRPNSVSYNIM 195
             + +F R+P F   R+++SLN LLN L+   +      +F+ + E  G  PN  + N++
Sbjct: 138 SSMRIFLRIPDFGVKRSVRSLNTLLNVLIQNQRFDLVHAMFKNSKESFGITPNIFTCNLL 197

Query: 196 IKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLGVLCRNGEMDTALCLFENMTQKGH 255
           +K   KK   E A  + DE+    + P++VTY + LG     G+M++A  + E M  +G 
Sbjct: 198 VKALCKKNDIESAYKVLDEIPSMGLVPNLVTYTTILGGYVARGDMESAKRVLEEMLDRGW 257

Query: 256 HPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLGKTGNIDEMES 315
           +P+A TY +LM+G+C +G++ EA  +M DME    +   V YGV++  L K     E  +
Sbjct: 258 YPDATTYTVLMDGYCKLGRFSEAATVMDDMEKNEIEPNEVTYGVMIRALCKEKKSGEARN 317

Query: 316 LLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAATYRMMIDGFC 375
           + +EM +R   PD      +++ LC++ K+ +A  +  KM    C P+ A    +I   C
Sbjct: 318 MFDEMLERSFMPDSSLCCKVIDALCEDHKVDEACGLWRKMLKNNCMPDNALLSTLIHWLC 377

Query: 376 NAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAE 435
             G    A K+ +     G  P L T+  L+ G+ +         + ++M +R+ + +A 
Sbjct: 378 KEGRVTEARKLFDE-FEKGSIPSLLTYNTLIAGMCEKGELTEAGRLWDDMYERKCKPNAF 437

Query: 436 TWRILV 439
           T+ +L+
Sbjct: 438 TYNVLI 442

BLAST of Lsi06G001520 vs. TAIR 10
Match: AT1G53330.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 7.1e-49
Identity = 128/446 (28.70%), Postives = 215/446 (48.21%), Query Frame = 0

Query: 56  VKEVKDPCEAMALFED------YRERGFKHHYPSYSSLIYKLARSRRFEAVETILGHLQS 115
           ++   DP  AM LF +        +R F++    Y  +I KL  S+ F+ ++ +L HL++
Sbjct: 16  LRRENDPSAAMKLFRNPDPESTNPKRPFRYSLLCYDIIITKLGGSKMFDELDQVLLHLKT 75

Query: 116 -RNIRCGETLFVALIQHYGKAHLVEKGIELFHRMPSFNCFRTLQSLNVLLNTLVDCDQLS 175
              I   E +F  +I  +G+  L  + + +F  MP + C RT++SLN LL+ L+ C +L 
Sbjct: 76  DTRIVPTEIIFCNVINFFGRGKLPSRALHMFDEMPQYRCQRTVKSLNSLLSALLKCGELE 135

Query: 176 KASEIFQQAYEMGFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFL 235
           K  E      E G +P++ +YNI+I G  + G ++ A  LFDEM++K+V+P+ VT+ + +
Sbjct: 136 KMKERLSSIDEFG-KPDACTYNILIHGCSQSGCFDDALKLFDEMVKKKVKPTGVTFGTLI 195

Query: 236 GVLCRNGEMDTALCLFENMTQ-KGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGC 295
             LC++  +  AL +  +M +  G  P    YA L++  C IG+   A KL  +      
Sbjct: 196 HGLCKDSRVKEALKMKHDMLKVYGVRPTVHIYASLIKALCQIGELSFAFKLKDEAYEGKI 255

Query: 296 KLRPVNYGVLMTHLGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYK 355
           K+    Y  L++ L K G  +E+  +L EM ++  KPD VTYN+L+N  C E     A +
Sbjct: 256 KVDAAIYSTLISSLIKAGRSNEVSMILEEMSEKGCKPDTVTYNVLINGFCVENDSESANR 315

Query: 356 VLIKM-QVG----------------------------------GCDPNAATYRMMIDGFC 415
           VL +M + G                                  GC P+  +YR++ DG C
Sbjct: 316 VLDEMVEKGLKPDVISYNMILGVFFRIKKWEEATYLFEDMPRRGCSPDTLSYRIVFDGLC 375

Query: 416 NAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAE 459
               F+ A  +L+ ML  G+ P        +  L +    + +  V+  +  R +  DA+
Sbjct: 376 EGLQFEEAAVILDEMLFKGYKPRRDRLEGFLQKLCESGKLEILSKVISSLH-RGIAGDAD 435

BLAST of Lsi06G001520 vs. TAIR 10
Match: AT5G18475.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.7e-47
Identity = 123/457 (26.91%), Postives = 227/457 (49.67%), Query Frame = 0

Query: 43  REKLREHIPFVTDVKEVKDPCEAMALF-EDYRERGFKHHYPSYSSLIYKLARSRRFEAVE 102
           + K   H   V+ +K  +DP   + +F +  +++GF H+  +YS L+  L R ++F AV+
Sbjct: 50  KTKFISHESAVSLMKRERDPQGVLDIFNKASQQKGFNHNNATYSVLLDNLVRHKKFLAVD 109

Query: 103 TILGHLQSRNIRCGETLFVALIQHYGKAHLVEKGIELFHRMPSFNCFR-TLQSLNVLLNT 162
            IL  ++    R  E+LF+ L++H+ ++ L +K +E+F+ +      + +L +++  LN 
Sbjct: 110 AILHQMKYETCRFQESLFLNLMRHFSRSDLHDKVMEMFNLIQVIARVKPSLNAISTCLNL 169

Query: 163 LVDCDQLSKASEIFQQA-YEMGFRPNSVSYNIMIKGWIKKGG------------------ 222
           L+D  +++ + ++   A + +G +PN+  +NI++K   K G                   
Sbjct: 170 LIDSGEVNLSRKLLLYAKHNLGLQPNTCIFNILVKHHCKNGDINFAFLVVEEMKRSGISY 229

Query: 223 ------------------WEQACNLFDEMLEKR-VQPSVVTYNSFLGVLCRNGEMDTALC 282
                              ++A  LF++M+ K  + P  VT+N  +   CR GE++ A  
Sbjct: 230 PNSITYSTLMDCLFAHSRSKEAVELFEDMISKEGISPDPVTFNVMINGFCRAGEVERAKK 289

Query: 283 LFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKLRPVNYGVLMTHLG 342
           + + M + G +PN   Y+ LM G+C +GK +EAK+   +++  G KL  V Y  LM    
Sbjct: 290 ILDFMKKNGCNPNVYNYSALMNGFCKVGKIQEAKQTFDEVKKTGLKLDTVGYTTLMNCFC 349

Query: 343 KTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDAYKVLIKMQVGGCDPNAA 402
           + G  DE   LL EMK  R + D +TYN+++  L  EG+  +A ++L +    G   N  
Sbjct: 350 RNGETDEAMKLLGEMKASRCRADTLTYNVILRGLSSEGRSEEALQMLDQWGSEGVHLNKG 409

Query: 403 TYRMMIDGFCNAGDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEM 458
           +YR++++  C  G+ + A+K L+ M   G  PH  T+  LV+ L +    +    VL   
Sbjct: 410 SYRIILNALCCNGELEKAVKFLSVMSERGIWPHHATWNELVVRLCESGYTEIGVRVLIGF 469

BLAST of Lsi06G001520 vs. TAIR 10
Match: AT4G20090.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 3.0e-47
Identity = 126/429 (29.37%), Postives = 202/429 (47.09%), Query Frame = 0

Query: 59  VKDPCEA---MALFEDYRERG-FKHHYPSYSSLIYKLARSRRFEAVETILGHLQSRNIRC 118
           V++P EA     +F+   + G FK    + SS+I   A S  F++VE +L  ++  N   
Sbjct: 51  VENPLEAPISEKMFKSAPKMGSFKLGDSTLSSMIESYANSGDFDSVEKLLSRIRLENRVI 110

Query: 119 GETLFVALIQHYGKAHLVEKGIELFHRM-PSFNCFRTLQSLNVLLNTLVDCDQLSKASEI 178
            E  F+ + + YGKAHL +K ++LFHRM   F C R+++S N +LN +++     +  E 
Sbjct: 111 IERSFIVVFRAYGKAHLPDKAVDLFHRMVDEFRCKRSVKSFNSVLNVIINEGLYHRGLEF 170

Query: 179 FQQA----YEMGFRPNSVSYNIMIKGWIKKGGWEQACNLFDEMLEKRVQPSVVTYNSFLG 238
           +         M   PN +S+N++IK   K    ++A  +F  M E++  P   TY + + 
Sbjct: 171 YDYVVNSNMNMNISPNGLSFNLVIKALCKLRFVDRAIEVFRGMPERKCLPDGYTYCTLMD 230

Query: 239 VLCRNGEMDTALCLFENMTQKGHHPNAITYALLMEGWCFIGKYKEAKKLMFDMEFRGCKL 298
            LC+   +D A+ L + M  +G  P+ + Y +L++G C  G      KL+ +M  +GC  
Sbjct: 231 GLCKEERIDEAVLLLDEMQSEGCSPSPVIYNVLIDGLCKKGDLTRVTKLVDNMFLKGCVP 290

Query: 299 RPVNYGVLMTHLGKTGNIDEMESLLNEMKKRRLKPDVVTYNILVNYLCKEGKIGDA---- 358
             V Y  L+  L   G +D+  SLL  M   +  P+ VTY  L+N L K+ +  DA    
Sbjct: 291 NEVTYNTLIHGLCLKGKLDKAVSLLERMVSSKCIPNDVTYGTLINGLVKQRRATDAVRLL 350

Query: 359 --------------YKVLI-----------------KMQVGGCDPNAATYRMMIDGFCNA 418
                         Y VLI                 KM   GC PN   Y +++DG C  
Sbjct: 351 SSMEERGYHLNQHIYSVLISGLFKEGKAEEAMSLWRKMAEKGCKPNIVVYSVLVDGLCRE 410

Query: 419 GDFDGAMKVLNAMLMSGHCPHLQTFVFLVLGLLKGENKDYVCFVLEEMEKRQLRFDAETW 444
           G  + A ++LN M+ SG  P+  T+  L+ G  K    +    V +EM+K     +   +
Sbjct: 411 GKPNEAKEILNRMIASGCLPNAYTYSSLMKGFFKTGLCEEAVQVWKEMDKTGCSRNKFCY 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQQ11.7e-14057.92Pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Arabidop... [more]
Q9FFE35.1e-5231.15Pentatricopeptide repeat-containing protein At5g16420, mitochondrial OS=Arabidop... [more]
Q9MAG89.9e-4828.70Putative pentatricopeptide repeat-containing protein At1g53330 OS=Arabidopsis th... [more]
Q3E9F02.4e-4626.91Pentatricopeptide repeat-containing protein At5g18475 OS=Arabidopsis thaliana OX... [more]
O494364.2e-4629.37Pentatricopeptide repeat-containing protein At4g20090 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A0A0M1K91.5e-24890.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570750 PE=4 SV=1[more]
A0A1S4DYZ62.2e-24490.67pentatricopeptide repeat-containing protein At1g07740, mitochondrial-like isofor... [more]
A0A5D3CEM18.5e-24490.44Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1HAJ52.6e-24088.45pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Cucurbit... [more]
A0A6J1HYX73.4e-24087.96pentatricopeptide repeat-containing protein At1g07740, mitochondrial OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
XP_038878835.11.6e-25291.90pentatricopeptide repeat-containing protein At1g07740, mitochondrial isoform X1 ... [more]
XP_011659903.13.1e-24890.41pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucumis sa... [more]
XP_023520779.17.1e-24589.52pentatricopeptide repeat-containing protein At1g07740, mitochondrial [Cucurbita ... [more]
XP_016900945.14.6e-24490.67PREDICTED: pentatricopeptide repeat-containing protein At1g07740, mitochondrial-... [more]
TYK10221.11.8e-24390.44pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G07740.11.2e-14157.92Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G16420.13.6e-5331.15Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT1G53330.17.1e-4928.70Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G18475.11.7e-4726.91Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G20090.13.0e-4729.37Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 56..205
e-value: 4.7E-26
score: 93.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 212..282
e-value: 2.0E-19
score: 71.9
coord: 283..354
e-value: 1.0E-17
score: 66.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 355..456
e-value: 1.3E-16
score: 62.5
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 294..326
e-value: 4.1E-7
score: 27.8
coord: 363..394
e-value: 2.8E-8
score: 31.4
coord: 119..148
e-value: 9.3E-4
score: 17.2
coord: 187..220
e-value: 7.5E-7
score: 26.9
coord: 222..256
e-value: 1.5E-8
score: 32.3
coord: 327..361
e-value: 5.3E-8
score: 30.6
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 119..147
e-value: 0.0074
score: 16.5
coord: 257..287
e-value: 0.028
score: 14.6
coord: 294..321
e-value: 3.7E-4
score: 20.5
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 184..233
e-value: 2.8E-16
score: 59.4
coord: 324..373
e-value: 6.8E-18
score: 64.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 150..184
score: 8.725252
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 360..394
score: 12.090371
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 255..289
score: 9.985802
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 185..219
score: 12.693243
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 325..359
score: 12.24383
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 220..254
score: 12.265752
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 290..324
score: 10.314641
NoneNo IPR availablePANTHERPTHR47942TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 26..457
NoneNo IPR availablePANTHERPTHR47942:SF41OS05G0548600 PROTEINcoord: 26..457
NoneNo IPR availableSUPERFAMILY81901HCP-likecoord: 160..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G001520.1Lsi06G001520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding