Lsi06G000820 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G000820 vs. ExPASy Swiss-Prot
Match: F4K2R6 (Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.4e-34
Identity = 103/294 (35.03%), Postives = 157/294 (53.40%), Query Frame = 0

Query: 69  INEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVFSTKEHESGKEEVATPSRTS 128
           + + M ++   +F ++    ME+  R+ V   I  S     S+      +    TPS  S
Sbjct: 29  VKKVMRDQSNNQFMIQ----MENMIRRIVREEIQRSLQPFLSSSCVSMERSRSETPSSRS 88

Query: 129 KYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGE 188
           + KL F+NS  S IFT ++I++EDG PL + + DAT +N+++STGP SS+RVE   L+ +
Sbjct: 89  RLKLCFINSPPSSIFTGSKIEAEDGSPLVIELVDAT-TNTLVSTGPFSSSRVELVPLNAD 148

Query: 189 FDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFV-NNLIVTDNSS 248
           F        E SWT   FN++ILT R+GKRPL+ G +  + LKNGVG +  ++  +DNSS
Sbjct: 149 F-------TEESWTVEGFNRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSS 208

Query: 249 WMKSKTFRLGVRVIDEKSL----AEFG--------------------------ISKDGTY 308
           W +S+ FRLG ++  + ++      FG                          I+KDG  
Sbjct: 209 WTRSRKFRLGAKLTGDGAVEARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVS 268

Query: 309 HKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECVVD 332
              L+ + I+TV DF + Y  N N   L  I+G  V  KTWN +V++A +CV+D
Sbjct: 269 ATRLAERKILTVKDFRRLYTVNRN--ELHNIIGAGVSKKTWNTIVSHAMDCVLD 307

BLAST of Lsi06G000820 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.8e-30
Identity = 87/320 (27.19%), Postives = 157/320 (49.06%), Query Frame = 0

Query: 50  FGTVFETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVF 109
           FG+ F  V E M  Q+     EP++E +++        K+  E  +   G       ++ 
Sbjct: 8   FGSKFSVVQEVMRLQTVKHFLEPVLEPLIR--------KVVKEEVELALGKHLAGIKWIC 67

Query: 110 STKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSI 169
             + H         P  +   +L+F+N++S  +FT+  I+ ++G+ ++V + D  ++  I
Sbjct: 68  EKETH---------PLESRNLQLKFLNNLSLPVFTSARIEGDEGQAIRVGLID-PSTGQI 127

Query: 170 ISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLY 229
            S+GP SS ++E  ++ G+F+S       + WT  D   +I+  R+GK+PL+ GN   + 
Sbjct: 128 FSSGPASSAKLEVFVVEGDFNS------VSDWTDEDIRNNIVREREGKKPLLNGNVFAV- 187

Query: 230 LKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF-------------------- 289
           L +G+G ++ +  TDNSSW +S+ FRLGVR++D+    +                     
Sbjct: 188 LNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQFDYVKIREAITESFVVRDHRGELYKK 247

Query: 290 --------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLD 336
                          I KDG +H+ L+   I TV DFL  +H N+  + LR +LG  +  
Sbjct: 248 HHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVKDFLTHFHLNS--SKLRQVLGTGMSS 300

BLAST of Lsi06G000820 vs. ExPASy Swiss-Prot
Match: Q0WVV6 (Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.9e-27
Identity = 76/235 (32.34%), Postives = 125/235 (53.19%), Query Frame = 0

Query: 131 KLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGEFD 190
           +L F + +S  +FT   ++ E G  + V + DA N+   ++ GP +S ++E  +L G+F+
Sbjct: 84  QLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDA-NTGRPVTVGPEASLKLEVVVLGGDFN 143

Query: 191 SSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFVNNLIVTDNSSWMK 250
           +    E++  WT  +F   ++  R+GKRPL+ G +  + LK GVG +  ++ TDNSSW++
Sbjct: 144 N----EDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTDNSSWIR 203

Query: 251 SKTFRLGVRV---------IDEKSLAEFG--------------------------ISKDG 310
           S+ FRLG+RV         I E     F                           I KDG
Sbjct: 204 SRKFRLGLRVPSGYCDGIRIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRLEKIGKDG 263

Query: 311 TYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECVV 331
            +HK L++ GI+TV  FL+   +++  T LR ILG  + +K W+++V +AK CV+
Sbjct: 264 AFHKRLTAAGIVTVEGFLRQLVRDS--TKLRAILGSGMSNKMWDLLVEHAKTCVL 310

BLAST of Lsi06G000820 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-26
Identity = 75/236 (31.78%), Postives = 121/236 (51.27%), Query Frame = 0

Query: 129 KYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGE 188
           K +L F + +S  +FT  +++ E G  + V + DA    +++  GP +S ++   +L G+
Sbjct: 93  KLQLHFKSRLSLPLFTGGKVEGEQGAVIHVVLIDANTGRAVV-YGPEASAKLHIVVLEGD 152

Query: 189 FDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFVNNLIVTDNSSW 248
           F++    E++  WT  +F   ++  R GKRPL+ G    + LK GVG +  L+ TDNSSW
Sbjct: 153 FNT----EDDEDWTQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTDNSSW 212

Query: 249 MKSKTFRLGVRVID---------EKSLAEF--------------------------GISK 308
           ++S+ FRLG+RV+          E     F                           I K
Sbjct: 213 IRSRKFRLGLRVVSGCCDGMRIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRLDKIGK 272

Query: 309 DGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECV 330
           DG +HK L+++GI TV DFL+   +++    LR ILG  + +K W+ +V +AK CV
Sbjct: 273 DGAFHKKLTAEGINTVEDFLRVMVKDS--PKLRTILGSGMSNKMWDALVEHAKTCV 320

BLAST of Lsi06G000820 vs. ExPASy Swiss-Prot
Match: F4JR57 (Calmodulin-binding protein 60 F OS=Arabidopsis thaliana OX=3702 GN=CBP60F PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.2e-25
Identity = 83/278 (29.86%), Postives = 132/278 (47.48%), Query Frame = 0

Query: 131 KLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGEFD 190
           +LRF   +   +FT  +++ E G  + V + DA N+ ++I TG  S T++   +L G+F+
Sbjct: 94  QLRFRTRMPPHLFTGGKVEGEQGSAIHVVLIDA-NTGNVIQTGEESMTKLNIVVLDGDFN 153

Query: 191 SSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFVNNLIVTDNSSWMK 250
                E++  WT   F    +  R+GKRP++ G +R + +K GVG +  L  TDNSSW++
Sbjct: 154 ----DEDDKDWTREHFESFEVKEREGKRPILTG-DRHVIIKEGVGTLGKLTFTDNSSWIR 213

Query: 251 SKTFRLGVRV-----IDEKSLAEFG--------------------------ISKDGTYHK 310
           S+ FRLGV+      I E     F                           I+KDG  HK
Sbjct: 214 SRKFRLGVKPATGFHIREAKTEPFAVKDHRGELYKKHYPPVLHDEVWRLDKIAKDGALHK 273

Query: 311 NLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECVVDVPINNDRT 370
            L    I+TV DFL+   +  +P  LR +LG  + ++ W+  V +AK CV+   +    T
Sbjct: 274 KLLKSNIVTVEDFLQILMK--DPQKLRSLLGSGMSNRMWDNTVEHAKTCVLGGKLYAYYT 333

Query: 371 LQANNDLVEMNQVMEDVAFQGNCGVMQNNLGNYDLAMS 378
            Q +   V  N + E      N   + +   N+D  +S
Sbjct: 334 DQTHQTAVVFNHIYEFQGLIANGHFLSSESLNHDQKIS 363

BLAST of Lsi06G000820 vs. ExPASy TrEMBL
Match: A0A1S3BQ76 (calmodulin-binding protein 60 G-like OS=Cucumis melo OX=3656 GN=LOC103492135 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 6.7e-96
Identity = 203/352 (57.67%), Postives = 246/352 (69.89%), Query Frame = 0

Query: 49  SFGTVFETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQ--------VYGS 108
           SF T  E VI+  + +SR  I EP + EVVKRF+ E WPKMEH+FRQQ        V+ +
Sbjct: 39  SFYTRREPVIDDAVTRSRSLIEEPRIGEVVKRFYDEIWPKMEHDFRQQVSKEIERMVHSA 98

Query: 109 IFLSFNYVFSTKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAI 168
           I  +F+   S ++    +EE+  PS+T K+KL F+NS  SVIFTNNE+KSEDGEPLKVAI
Sbjct: 99  IHSAFSPSLSIQQ-GCEQEEILKPSKTLKFKLCFLNSFPSVIFTNNEVKSEDGEPLKVAI 158

Query: 169 CDATNSNSIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPL 228
           CDATNSNSI+STGPLSS +VE  IL GEFDSS  ++EET WTSS FNKSILTPRDGKRPL
Sbjct: 159 CDATNSNSIVSTGPLSSAQVELCILPGEFDSSNRRDEETPWTSSYFNKSILTPRDGKRPL 218

Query: 229 IIGNNRQLYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF----------- 288
           IIG++RQ+YLK+GV  +NNLI+TDNSSWMKSK FRLGV++ DE+ LAEF           
Sbjct: 219 IIGDDRQVYLKDGVRLINNLIITDNSSWMKSKKFRLGVKIKDERVLAEFVRIGEAISEPF 278

Query: 289 --------------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPT 348
                                     GI+KDG YHKNLSS+GI TVGDFLKAYHQ N+P 
Sbjct: 279 RVMDQRGEVNQKHHPPRWEDEVWRLEGIAKDGIYHKNLSSKGIRTVGDFLKAYHQ-NDPD 338

Query: 349 TLRMILGKRVLDKTWNMMVNNAKECVVDVPINNDRTLQANNDLVEMNQVMED 356
           TLRM+LGKRVLDKTW MMV NA ECV D   +N++  Q N+  +E NQV+++
Sbjct: 339 TLRMMLGKRVLDKTWKMMVQNACECVEDT-FDNNQVFQPNS-FLEFNQVLDE 386

BLAST of Lsi06G000820 vs. ExPASy TrEMBL
Match: A0A5D3CGF5 (Calmodulin-binding protein 60 G-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004240 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 6.7e-96
Identity = 203/352 (57.67%), Postives = 246/352 (69.89%), Query Frame = 0

Query: 49  SFGTVFETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQ--------VYGS 108
           SF T  E VI+  + +SR  I EP + EVVKRF+ E WPKMEH+FRQQ        V+ +
Sbjct: 39  SFYTRREPVIDDAVTRSRSLIEEPRIGEVVKRFYDEIWPKMEHDFRQQVSKEIERMVHSA 98

Query: 109 IFLSFNYVFSTKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAI 168
           I  +F+   S ++    +EE+  PS+T K+KL F+NS  SVIFTNNE+KSEDGEPLKVAI
Sbjct: 99  IHSAFSPSLSIQQ-GCEQEEILKPSKTLKFKLCFLNSFPSVIFTNNEVKSEDGEPLKVAI 158

Query: 169 CDATNSNSIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPL 228
           CDATNSNSI+STGPLSS +VE  IL GEFDSS  ++EET WTSS FNKSILTPRDGKRPL
Sbjct: 159 CDATNSNSIVSTGPLSSAQVELCILPGEFDSSNRRDEETPWTSSYFNKSILTPRDGKRPL 218

Query: 229 IIGNNRQLYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF----------- 288
           IIG++RQ+YLK+GV  +NNLI+TDNSSWMKSK FRLGV++ DE+ LAEF           
Sbjct: 219 IIGDDRQVYLKDGVRLINNLIITDNSSWMKSKKFRLGVKIKDERVLAEFVRIGEAISEPF 278

Query: 289 --------------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPT 348
                                     GI+KDG YHKNLSS+GI TVGDFLKAYHQ N+P 
Sbjct: 279 RVMDQRGEVNQKHHPPRWEDEVWRLEGIAKDGIYHKNLSSKGIRTVGDFLKAYHQ-NDPD 338

Query: 349 TLRMILGKRVLDKTWNMMVNNAKECVVDVPINNDRTLQANNDLVEMNQVMED 356
           TLRM+LGKRVLDKTW MMV NA ECV D   +N++  Q N+  +E NQV+++
Sbjct: 339 TLRMMLGKRVLDKTWKMMVQNACECVEDT-FDNNQVFQPNS-FLEFNQVLDE 386

BLAST of Lsi06G000820 vs. ExPASy TrEMBL
Match: A0A6J1I0G5 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.8e-53
Identity = 139/342 (40.64%), Postives = 185/342 (54.09%), Query Frame = 0

Query: 94  RQQVYGSIFLSFNY-----VFSTKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEI 153
           R  V+  + L  NY     + S   +  GKE        SK+KL F+N  +  IFTNNEI
Sbjct: 58  RSVVHEVVELQVNYYIRSILSSQLSNHIGKEATINGFGASKFKLLFLNQTAPTIFTNNEI 117

Query: 154 KSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNK 213
           ++ +G+PL VAICD TNSN II TGPLSS  VE  +L+ EF S + + +E+SWTS DFN 
Sbjct: 118 EARNGKPLLVAICDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQ-RVDESSWTSRDFNN 177

Query: 214 SILTPRDGKRPLIIGNNRQLYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAE 273
           ++++ R+GKRPLI+GN+  + LKNGVGF+  L  +DNSSW KSK FRLG +V+D+  LA+
Sbjct: 178 NVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNILAK 237

Query: 274 F-------------------------------------GISKDGTYHKNLSSQGIMTVGD 333
           F                                     GI KDG YH  L S+GI  V D
Sbjct: 238 FPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSKGIKNVDD 297

Query: 334 FLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKEC---VVDVPINNDRTLQANNDLVE 391
           FLKAYH+   PT  R +LG RV   TW  MVNNA EC   +  +  N    +++      
Sbjct: 298 FLKAYHK-KGPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVRSMG---- 357

BLAST of Lsi06G000820 vs. ExPASy TrEMBL
Match: A0A6J1HYX2 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.1e-53
Identity = 139/344 (40.41%), Postives = 184/344 (53.49%), Query Frame = 0

Query: 94  RQQVYGSIFLSFNYVF-------STKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNN 153
           R  V+  + L  NY         S   +  GKE        SK+KL F+N  +  IFTNN
Sbjct: 58  RSVVHEVVELQVNYYIRSILSRNSQLSNHIGKEATINGFGASKFKLLFLNQTAPTIFTNN 117

Query: 154 EIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDF 213
           EI++ +G+PL VAICD TNSN II TGPLSS  VE  +L+ EF S + + +E+SWTS DF
Sbjct: 118 EIEARNGKPLLVAICDTTNSNGIIKTGPLSSALVELVVLNLEFGSDQ-RVDESSWTSRDF 177

Query: 214 NKSILTPRDGKRPLIIGNNRQLYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSL 273
           N ++++ R+GKRPLI+GN+  + LKNGVGF+  L  +DNSSW KSK FRLG +V+D+  L
Sbjct: 178 NNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTKSKKFRLGAKVVDKNIL 237

Query: 274 AEF-------------------------------------GISKDGTYHKNLSSQGIMTV 333
           A+F                                     GI KDG YH  L S+GI  V
Sbjct: 238 AKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGKDGIYHNRLFSKGIKNV 297

Query: 334 GDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKEC---VVDVPINNDRTLQANNDL 391
            DFLKAYH+   PT  R +LG RV   TW  MVNNA EC   +  +  N    +++    
Sbjct: 298 DDFLKAYHK-KGPTYFRKLLGGRVPQNTWKRMVNNALECDAFIASMASNPSFRVRSMG-- 357

BLAST of Lsi06G000820 vs. ExPASy TrEMBL
Match: A0A6J1HBN4 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461979 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 2.0e-52
Identity = 133/310 (42.90%), Postives = 174/310 (56.13%), Query Frame = 0

Query: 118 KEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSS 177
           KEE       SK+KL F+N  S  IFTNNEI++ +G+PL VA+CD TNSN II TGPLSS
Sbjct: 87  KEETINGFGASKFKLLFLNQTSPTIFTNNEIEARNGKPLLVAMCDTTNSNGIIKTGPLSS 146

Query: 178 TRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFV 237
             VE  +L+ EF S + + +E+SWTSSDFN ++++ R+GKRPLI+GN+  + LKNGVGF+
Sbjct: 147 ALVELVVLNLEFGSDQ-RVDESSWTSSDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFI 206

Query: 238 NNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF---------------------------- 297
             L  +DNSSW KSK FRLG +V+D+  LA+F                            
Sbjct: 207 KGLSFSDNSSWTKSKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGEVYKKHHPPS 266

Query: 298 ---------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNM 357
                    GI KDG YH  LSS+GI  V DFL AYH+   P  +R +LG RV   TW  
Sbjct: 267 REDEIWRLEGIGKDGVYHNRLSSEGIKNVDDFLNAYHK-KGPIYVRKLLGGRVPQNTWKR 326

Query: 358 MVNNAKECVVDVPINNDRTLQANNDLVEMNQVMEDVAFQGNCGVMQNNLGNYDLAMSLVD 391
           MVNNA EC  D  I +  +  +       N+ M   AF  N  V +    N   +  L  
Sbjct: 327 MVNNALEC--DAFIASMASNPSFRVCSMGNEAMGGEAFDMNSEVSEPK--NCSESDGLQF 386

BLAST of Lsi06G000820 vs. NCBI nr
Match: XP_038879917.1 (calmodulin-binding protein 60 G-like isoform X2 [Benincasa hispida])

HSP 1 Score: 445.7 bits (1145), Expect = 4.3e-121
Identity = 252/389 (64.78%), Postives = 284/389 (73.01%), Query Frame = 0

Query: 52  TVFETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSI---FLSFNYV 111
           T F  V EHM+A+  PQI EP+M EV+ R FVE WPKMEHEFRQQV   I    +S  Y 
Sbjct: 40  TCFGAVFEHMVARWWPQIKEPVMGEVLNRIFVETWPKMEHEFRQQVSKEIERMMISRIYS 99

Query: 112 FS---TKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATN 171
            S   +++HES + EV T S+T K KL F NSIS +IFTNNEIKSE GEPLKV I DATN
Sbjct: 100 LSPSPSEQHESEEGEVPTTSKTLKLKLCFSNSISPLIFTNNEIKSEHGEPLKVLIHDATN 159

Query: 172 SNSIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNN 231
           SN+IISTGP SS  VEF IL GEFDSSK Q++ET WTSSDF+KSILTPRDGKRPLIIG++
Sbjct: 160 SNTIISTGPSSSALVEFFIL-GEFDSSKRQDDETPWTSSDFDKSILTPRDGKRPLIIGSD 219

Query: 232 RQLYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF---------------- 291
           RQLYL+NGVGFV NLIVTDNSSWMKSK FRLG R+ DEKS  +F                
Sbjct: 220 RQLYLQNGVGFVKNLIVTDNSSWMKSKMFRLGARIKDEKSQVQFGRIGEAVSRPFRVMDQ 279

Query: 292 ---------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMI 351
                                GISKDG YHKNLSSQGI TVGDFLKAY +NNNPTTLRM+
Sbjct: 280 RGEGNQKHHPPRREDEIWRLEGISKDGIYHKNLSSQGIKTVGDFLKAYQKNNNPTTLRMM 339

Query: 352 LGKRVLDKTWNMMVNNAKECVVD-VPINNDRTLQANNDLVEMNQVMEDVAFQGNCGVMQN 396
           LGKRVLDKTWNMMV NA+EC+VD VPIN+DRT+QANN L E+NQVM D AFQGN G MQN
Sbjct: 340 LGKRVLDKTWNMMVKNAEECIVDNVPINDDRTVQANN-LAEINQVMGDEAFQGNFGFMQN 399

BLAST of Lsi06G000820 vs. NCBI nr
Match: XP_038879916.1 (calmodulin-binding protein 60 G-like isoform X1 [Benincasa hispida])

HSP 1 Score: 438.0 bits (1125), Expect = 8.9e-119
Identity = 249/385 (64.68%), Postives = 280/385 (72.73%), Query Frame = 0

Query: 52  TVFETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSI---FLSFNYV 111
           T F  V EHM+A+  PQI EP+M EV+ R FVE WPKMEHEFRQQV   I    +S  Y 
Sbjct: 40  TCFGAVFEHMVARWWPQIKEPVMGEVLNRIFVETWPKMEHEFRQQVSKEIERMMISRIYS 99

Query: 112 FS---TKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATN 171
            S   +++HES + EV T S+T K KL F NSIS +IFTNNEIKSE GEPLKV I DATN
Sbjct: 100 LSPSPSEQHESEEGEVPTTSKTLKLKLCFSNSISPLIFTNNEIKSEHGEPLKVLIHDATN 159

Query: 172 SNSIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNN 231
           SN+IISTGP SS  VEF IL GEFDSSK Q++ET WTSSDF+KSILTPRDGKRPLIIG++
Sbjct: 160 SNTIISTGPSSSALVEFFIL-GEFDSSKRQDDETPWTSSDFDKSILTPRDGKRPLIIGSD 219

Query: 232 RQLYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF---------------- 291
           RQLYL+NGVGFV NLIVTDNSSWMKSK FRLG R+ DEKS  +F                
Sbjct: 220 RQLYLQNGVGFVKNLIVTDNSSWMKSKMFRLGARIKDEKSQVQFGRIGEAVSRPFRVMDQ 279

Query: 292 ---------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMI 351
                                GISKDG YHKNLSSQGI TVGDFLKAY +NNNPTTLRM+
Sbjct: 280 RGEGNQKHHPPRREDEIWRLEGISKDGIYHKNLSSQGIKTVGDFLKAYQKNNNPTTLRMM 339

Query: 352 LGKRVLDKTWNMMVNNAKECVVD-VPINNDRTLQANNDLVEMNQVMEDVAFQGNCGVMQN 392
           LGKRVLDKTWNMMV NA+EC+VD VPIN+DRT+QANN L E+NQVM D AFQGN G MQN
Sbjct: 340 LGKRVLDKTWNMMVKNAEECIVDNVPINDDRTVQANN-LAEINQVMGDEAFQGNFGFMQN 399

BLAST of Lsi06G000820 vs. NCBI nr
Match: XP_004135591.1 (calmodulin-binding protein 60 G isoform X3 [Cucumis sativus])

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-96
Identity = 205/385 (53.25%), Postives = 256/385 (66.49%), Query Frame = 0

Query: 55  ETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVFSTK-- 114
           ++++E  + +SR Q+ EP + EVVKRF+ E WPK+E++FRQQV+  +    +    +   
Sbjct: 46  KSIVEDAVTRSRSQMEEPRIGEVVKRFYDEIWPKVENDFRQQVFKEVQRMIHSAIHSAIS 105

Query: 115 -----EHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSN 174
                +H S +EE+  PS+T K +L F+NS  SVIFTNNEIKSEDGEPLKVAICD TN N
Sbjct: 106 PSLSIQHRSEQEEILKPSKTLKRRLFFLNSFPSVIFTNNEIKSEDGEPLKVAICDTTNCN 165

Query: 175 SIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQ 234
           SI+ST PLSS +VE  IL GEFDSS  ++EET WTSS FN SILTPRDGKRPLIIGN+RQ
Sbjct: 166 SIVSTSPLSSVQVELCILPGEFDSSNRRDEETPWTSSYFNTSILTPRDGKRPLIIGNDRQ 225

Query: 235 LYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF------------------ 294
           +YLK+GVGF+NNLI+TDNSSWMKSK FRLG ++ DE+  A F                  
Sbjct: 226 VYLKDGVGFINNLIITDNSSWMKSKKFRLGAKITDERIPAGFGRIGEAVSQPFRVMDQRG 285

Query: 295 -------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILG 354
                              GI+K G YHKNLSSQGI TVGDFLKAYHQNNNP TLR +LG
Sbjct: 286 EVNQKHHPPRMDDEVWRLEGIAKYGIYHKNLSSQGIKTVGDFLKAYHQNNNPNTLRTMLG 345

Query: 355 KRVLDKTWNMMVNNAKECVVDVPINNDRTLQANNDLVEMNQVMEDVAFQGNCGVMQNNLG 396
           KRVLDKTW MMV NA+ECV  VPI+N++ +QAN   V+ N VM+D A + + G+M + + 
Sbjct: 346 KRVLDKTWKMMVQNAEECV--VPIDNNQIVQANGP-VKFNHVMDDSAIK-DFGLMSDFIE 405

BLAST of Lsi06G000820 vs. NCBI nr
Match: XP_031743132.1 (calmodulin-binding protein 60 G isoform X1 [Cucumis sativus])

HSP 1 Score: 363.6 bits (932), Expect = 2.1e-96
Identity = 197/349 (56.45%), Postives = 241/349 (69.05%), Query Frame = 0

Query: 55  ETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVFSTK-- 114
           ++++E  + +SR Q+ EP + EVVKRF+ E WPK+E++FRQQV+  +    +    +   
Sbjct: 46  KSIVEDAVTRSRSQMEEPRIGEVVKRFYDEIWPKVENDFRQQVFKEVQRMIHSAIHSAIS 105

Query: 115 -----EHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSN 174
                +H S +EE+  PS+T K +L F+NS  SVIFTNNEIKSEDGEPLKVAICD TN N
Sbjct: 106 PSLSIQHRSEQEEILKPSKTLKRRLFFLNSFPSVIFTNNEIKSEDGEPLKVAICDTTNCN 165

Query: 175 SIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQ 234
           SI+ST PLSS +VE  IL GEFDSS  ++EET WTSS FN SILTPRDGKRPLIIGN+RQ
Sbjct: 166 SIVSTSPLSSVQVELCILPGEFDSSNRRDEETPWTSSYFNTSILTPRDGKRPLIIGNDRQ 225

Query: 235 LYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF------------------ 294
           +YLK+GVGF+NNLI+TDNSSWMKSK FRLG ++ DE+  A F                  
Sbjct: 226 VYLKDGVGFINNLIITDNSSWMKSKKFRLGAKITDERIPAGFGRIGEAVSQPFRVMDQRG 285

Query: 295 -------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILG 354
                              GI+K G YHKNLSSQGI TVGDFLKAYHQNNNP TLR +LG
Sbjct: 286 EVNQKHHPPRMDDEVWRLEGIAKYGIYHKNLSSQGIKTVGDFLKAYHQNNNPNTLRTMLG 345

Query: 355 KRVLDKTWNMMVNNAKECVVDVPINNDRTLQANNDLVEMNQVMEDVAFQ 360
           KRVLDKTW MMV NA+ECV  VPI+N++ +QAN   V+ N VM+D A +
Sbjct: 346 KRVLDKTWKMMVQNAEECV--VPIDNNQIVQANGP-VKFNHVMDDSAIK 391

BLAST of Lsi06G000820 vs. NCBI nr
Match: XP_011659742.1 (calmodulin-binding protein 60 G isoform X2 [Cucumis sativus])

HSP 1 Score: 363.6 bits (932), Expect = 2.1e-96
Identity = 197/349 (56.45%), Postives = 241/349 (69.05%), Query Frame = 0

Query: 55  ETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVFSTK-- 114
           ++++E  + +SR Q+ EP + EVVKRF+ E WPK+E++FRQQV+  +    +    +   
Sbjct: 46  KSIVEDAVTRSRSQMEEPRIGEVVKRFYDEIWPKVENDFRQQVFKEVQRMIHSAIHSAIS 105

Query: 115 -----EHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSN 174
                +H S +EE+  PS+T K +L F+NS  SVIFTNNEIKSEDGEPLKVAICD TN N
Sbjct: 106 PSLSIQHRSEQEEILKPSKTLKRRLFFLNSFPSVIFTNNEIKSEDGEPLKVAICDTTNCN 165

Query: 175 SIISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQ 234
           SI+ST PLSS +VE  IL GEFDSS  ++EET WTSS FN SILTPRDGKRPLIIGN+RQ
Sbjct: 166 SIVSTSPLSSVQVELCILPGEFDSSNRRDEETPWTSSYFNTSILTPRDGKRPLIIGNDRQ 225

Query: 235 LYLKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF------------------ 294
           +YLK+GVGF+NNLI+TDNSSWMKSK FRLG ++ DE+  A F                  
Sbjct: 226 VYLKDGVGFINNLIITDNSSWMKSKKFRLGAKITDERIPAGFGRIGEAVSQPFRVMDQRG 285

Query: 295 -------------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILG 354
                              GI+K G YHKNLSSQGI TVGDFLKAYHQNNNP TLR +LG
Sbjct: 286 EVNQKHHPPRMDDEVWRLEGIAKYGIYHKNLSSQGIKTVGDFLKAYHQNNNPNTLRTMLG 345

Query: 355 KRVLDKTWNMMVNNAKECVVDVPINNDRTLQANNDLVEMNQVMEDVAFQ 360
           KRVLDKTW MMV NA+ECV  VPI+N++ +QAN   V+ N VM+D A +
Sbjct: 346 KRVLDKTWKMMVQNAEECV--VPIDNNQIVQANGP-VKFNHVMDDSAIK 391

BLAST of Lsi06G000820 vs. TAIR 10
Match: AT5G26920.1 (Cam-binding protein 60-like G )

HSP 1 Score: 148.7 bits (374), Expect = 1.0e-35
Identity = 103/294 (35.03%), Postives = 157/294 (53.40%), Query Frame = 0

Query: 69  INEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVFSTKEHESGKEEVATPSRTS 128
           + + M ++   +F ++    ME+  R+ V   I  S     S+      +    TPS  S
Sbjct: 29  VKKVMRDQSNNQFMIQ----MENMIRRIVREEIQRSLQPFLSSSCVSMERSRSETPSSRS 88

Query: 129 KYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGE 188
           + KL F+NS  S IFT ++I++EDG PL + + DAT +N+++STGP SS+RVE   L+ +
Sbjct: 89  RLKLCFINSPPSSIFTGSKIEAEDGSPLVIELVDAT-TNTLVSTGPFSSSRVELVPLNAD 148

Query: 189 FDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFV-NNLIVTDNSS 248
           F        E SWT   FN++ILT R+GKRPL+ G +  + LKNGVG +  ++  +DNSS
Sbjct: 149 F-------TEESWTVEGFNRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSS 208

Query: 249 WMKSKTFRLGVRVIDEKSL----AEFG--------------------------ISKDGTY 308
           W +S+ FRLG ++  + ++      FG                          I+KDG  
Sbjct: 209 WTRSRKFRLGAKLTGDGAVEARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVS 268

Query: 309 HKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECVVD 332
              L+ + I+TV DF + Y  N N   L  I+G  V  KTWN +V++A +CV+D
Sbjct: 269 ATRLAERKILTVKDFRRLYTVNRN--ELHNIIGAGVSKKTWNTIVSHAMDCVLD 307

BLAST of Lsi06G000820 vs. TAIR 10
Match: AT5G26920.2 (Cam-binding protein 60-like G )

HSP 1 Score: 142.1 bits (357), Expect = 9.4e-34
Identity = 100/294 (34.01%), Postives = 154/294 (52.38%), Query Frame = 0

Query: 69  INEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVFSTKEHESGKEEVATPSRTS 128
           + + M ++   +F ++    ME+  R+ V   I  S     S+      +    TPS  S
Sbjct: 29  VKKVMRDQSNNQFMIQ----MENMIRRIVREEIQRSLQPFLSSSCVSMERSRSETPSSRS 88

Query: 129 KYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGE 188
           + KL F+NS  S IFT ++I++EDG PL + + DAT +N+++STGP SS+RVE   L+ +
Sbjct: 89  RLKLCFINSPPSSIFTGSKIEAEDGSPLVIELVDAT-TNTLVSTGPFSSSRVELVPLNAD 148

Query: 189 FDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFV-NNLIVTDNSS 248
           F        E SWT   FN++ILT R+GKRPL+ G +  + LKNGVG +  ++  +DNSS
Sbjct: 149 F-------TEESWTVEGFNRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSS 208

Query: 249 WMKSKTFRLGVRVIDEKSL----AEFG--------------------------ISKDGTY 308
           W +S+ FRLG ++  + ++      FG                          I+KDG  
Sbjct: 209 WTRSRKFRLGAKLTGDGAVEARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVS 268

Query: 309 HKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECVVD 332
              L+ + I+TV DF + Y           I+G  V  KTWN +V++A +CV+D
Sbjct: 269 ATRLAERKILTVKDFRRLY----------TIIGAGVSKKTWNTIVSHAMDCVLD 299

BLAST of Lsi06G000820 vs. TAIR 10
Match: AT5G62570.2 (Calmodulin binding protein-like )

HSP 1 Score: 134.4 bits (337), Expect = 2.0e-31
Identity = 87/320 (27.19%), Postives = 157/320 (49.06%), Query Frame = 0

Query: 50  FGTVFETVIEHMIAQSRPQINEPMMEEVVKRFFVEKWPKMEHEFRQQVYGSIFLSFNYVF 109
           FG+ F  V E M  Q+     EP++E +++        K+  E  +   G       ++ 
Sbjct: 8   FGSKFSVVQEVMRLQTVKHFLEPVLEPLIR--------KVVKEEVELALGKHLAGIKWIC 67

Query: 110 STKEHESGKEEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSI 169
             + H         P  +   +L+F+N++S  +FT+  I+ ++G+ ++V + D  ++  I
Sbjct: 68  EKETH---------PLESRNLQLKFLNNLSLPVFTSARIEGDEGQAIRVGLID-PSTGQI 127

Query: 170 ISTGPLSSTRVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLY 229
            S+GP SS ++E  ++ G+F+S       + WT  D   +I+  R+GK+PL+ GN   + 
Sbjct: 128 FSSGPASSAKLEVFVVEGDFNS------VSDWTDEDIRNNIVREREGKKPLLNGNVFAV- 187

Query: 230 LKNGVGFVNNLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF-------------------- 289
           L +G+G ++ +  TDNSSW +S+ FRLGVR++D+    +                     
Sbjct: 188 LNDGIGVMDEISFTDNSSWTRSRKFRLGVRIVDQFDYVKIREAITESFVVRDHRGELYKK 247

Query: 290 --------------GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLD 336
                          I KDG +H+ L+   I TV DFL  +H N+  + LR +LG  +  
Sbjct: 248 HHPPSLFDEVWRLEKIGKDGAFHRRLNLSNINTVKDFLTHFHLNS--SKLRQVLGTGMSS 300

BLAST of Lsi06G000820 vs. TAIR 10
Match: AT5G62570.1 (Calmodulin binding protein-like )

HSP 1 Score: 129.8 bits (325), Expect = 4.8e-30
Identity = 74/251 (29.48%), Postives = 133/251 (52.99%), Query Frame = 0

Query: 119 EEVATPSRTSKYKLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSST 178
           E+   P  +   +L+F+N++S  +FT+  I+ ++G+ ++V + D  ++  I S+GP SS 
Sbjct: 42  EKETHPLESRNLQLKFLNNLSLPVFTSARIEGDEGQAIRVGLID-PSTGQIFSSGPASSA 101

Query: 179 RVEFSILHGEFDSSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFVN 238
           ++E  ++ G+F+S       + WT  D   +I+  R+GK+PL+ GN   + L +G+G ++
Sbjct: 102 KLEVFVVEGDFNS------VSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMD 161

Query: 239 NLIVTDNSSWMKSKTFRLGVRVIDEKSLAEF----------------------------- 298
            +  TDNSSW +S+ FRLGVR++D+    +                              
Sbjct: 162 EISFTDNSSWTRSRKFRLGVRIVDQFDYVKIREAITESFVVRDHRGELYKKHHPPSLFDE 221

Query: 299 -----GISKDGTYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNN 336
                 I KDG +H+ L+   I TV DFL  +H N+  + LR +LG  +  K W + +++
Sbjct: 222 VWRLEKIGKDGAFHRRLNLSNINTVKDFLTHFHLNS--SKLRQVLGTGMSSKMWEITLDH 281

BLAST of Lsi06G000820 vs. TAIR 10
Match: AT4G25800.1 (Calmodulin-binding protein )

HSP 1 Score: 123.6 bits (309), Expect = 3.5e-28
Identity = 76/235 (32.34%), Postives = 125/235 (53.19%), Query Frame = 0

Query: 131 KLRFVNSISSVIFTNNEIKSEDGEPLKVAICDATNSNSIISTGPLSSTRVEFSILHGEFD 190
           +L F + +S  +FT   ++ E G  + V + DA N+   ++ GP +S ++E  +L G+F+
Sbjct: 84  QLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDA-NTGRPVTVGPEASLKLEVVVLGGDFN 143

Query: 191 SSKWQEEETSWTSSDFNKSILTPRDGKRPLIIGNNRQLYLKNGVGFVNNLIVTDNSSWMK 250
           +    E++  WT  +F   ++  R+GKRPL+ G +  + LK GVG +  ++ TDNSSW++
Sbjct: 144 N----EDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTDNSSWIR 203

Query: 251 SKTFRLGVRV---------IDEKSLAEFG--------------------------ISKDG 310
           S+ FRLG+RV         I E     F                           I KDG
Sbjct: 204 SRKFRLGLRVPSGYCDGIRIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRLEKIGKDG 263

Query: 311 TYHKNLSSQGIMTVGDFLKAYHQNNNPTTLRMILGKRVLDKTWNMMVNNAKECVV 331
            +HK L++ GI+TV  FL+   +++  T LR ILG  + +K W+++V +AK CV+
Sbjct: 264 AFHKRLTAAGIVTVEGFLRQLVRDS--TKLRAILGSGMSNKMWDLLVEHAKTCVL 310