Lsi06G000310 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G000310
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein kinase superfamily protein
Locationchr06: 334009 .. 339024 (+)
RNA-Seq ExpressionLsi06G000310
SyntenyLsi06G000310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGACACCTATTTTTATTTTGCTGGGCTCTTCTTTTTCTTTGACTGTCACCAGCGCATTATAGCATATACCACAGTCCACAACAAATACAATACAAATAAAGATTAAAAAAAAAAAAAAAAACTCTCTCTTTCCATTCCTTTCCTTCTCTCTCTCCTAACGCAAAATCCAAAAGGGAAAACCCAGTTCTTCACATCGCACACCCTACGAAACTTTCTCGCGGATCTGACCCTTTAATTTTCTCCCACCAACCTGCCTCTCTGTTTTCTCCTCCATGTCTCAAGATTTTACATTCCATCGGTAATGGGTTTCTTCTTTTGATTCCGTTTCTGTATTCTATTTTTTTTAAAAAAAAAATTCTCGTTTTTTTTTTTCTCCCTTTTAATGGGTTTTCTGTATCTGGATGGTGGTGTTCTTGGGTAGGATCATTTCGTGTCATTTATATGGATCACCTACTTGTTGATGTTTTTCTTTCTCCTCTGTTTTTATGTTCGTTTGTGGTTCGATGATTTTCGCAGCTTCCGACGGGATTGGTGGGTTAATTATCTAATATTTTATGTTGTATTTGCTTTTTTATGACCTCGAGTCTTTAGTTTCGAGCTCTATTTCTCCATATGGTGCTTTTATGTTATGTTGACCTATACCCTTGTTTGAGGTCAGATCGCAATTTGTTTCTTGCGCGGATTTCTTGAGAAGGTTCTTTACCGTTTAGAGATGTTTTGGATGAATAATGGTTTGAGCCGAATGTTTATAGAGGCGAGAGAATGGCAGATATGAAGATGAACACCTTTTGGGTATTTGGTTTCATTTTCTGCAAAATGGTCGATGAATAAAGAAGCAGAAATAGTTGTATGATTTTTCTGGACTTTGTAGTGGATTCATGTGCCCGATTTAGCTTAGTGTTTTTCTTCTTCTGTTCTAGGAGTTGAGAATTTCCAGGAAAGTGCAGCGTGGAAAATTAAGTCTAAATTGTAGTGAAGAAAGAATTATTCTTCCCAGACAGGGTTAAATGGTTGATGCAGATTATTAATATTTCACGGGAGTTGTGTCAAAAGGATTGAGTAAGGTGGTAGATGTTTTTGGAATGAAGATATATGATTAAGTTATAACTTATCTGATTTGATTTCAACAGGTGAATGGTTAGTTTTATAAGGATCATTCACTGAAGAAAGCTCGTGACGATTTCAAGAAATGGCTTCTATGGGTGTTGTTTCTACTTCAAGTTTGAGAGAGCCAGCTGGTCATAATGTTGGTGTTGATAGGTTACCCGAAGAGATGAATGACATGAAAATTAGAGATGACAAGGTACGCTTGGGTACTTTGATGCCGTTAACTTTCATTTCTAATTGTATTGTTTACCTGAGTTAGTTTTGAATTTGTTCTTGTGCTTTTATATGAGTTCTCATAAGTTGAAACAATGGCATAGGAAGTGGAAGCAACAGTTGTAGATGGCAATGGGACAGAGACAGGCCATATAATTGTGACAACAATTGGTGGTCGAAATGGCTACTCAAAGCAGGCATGAAATTCATGATAAATTCTTCCTTGTATCACATTCTTTTTAAGTGTGTGCACTCGTCTGAGTGGGCTGACCAACATTTTCTAGTGTGAGATTGCATATATTATATGTTGGTCATTCCTCTTTGCTTCTAAGTGATGAAATTCATTTTACATTTTGTACGTATTATCTTTTAGTCACTGTTTGTTGTCCCTCTAAGGGTATGTGGTATTGCAATGCAGACAATCAGCTACATGGCAGAACGTGCTGTTGGGCATGGATCATTTGGAGTCGTGTTTCAAGTAATGTTTGCAATTGTTTTCCCAACTGATAGTGGCTTTTTACCTCTTCATGTTTCTAAAAAATTACCCTTTTTAAGGGTTTTTCAGATATTGAGGATGCACACTTTGTATATGCATTTACTATTTTCTGTATCTTGTTTAGGCAAAGTGCTTAGAGACTGGGGAAACTGTGGCTATAAAGAAGGTTCTCCAAGACAAAAGGTACAAGAACCGTGAGCTGCAAACTATGCGGCTTCTTGACCATCCAAATGTTGTTGCATTAAAGCATTGCTTCTTCTCAACCACCGAAAAGGATGAACTATATCTCAACCTGGTTCTTGAGTATGTCCCTGAAACAGTTCATAGGGTGATAAAGCACTACAACAAGTTGAATCAAAGGATGCCACTGATCTATGTAAAACTTTACATGTACCAGGTATTGGTCTTCTGAATTTCTTTTCAAGAAACGTAATATTTGATAGTTAGTTAATGAGAAAGAAAACCCATATTAGATCCATTCCATTTCATTCAGTTTGGTTTGAAGTTAAACTACCATTTTTGATGCACTTTTATTACTCATTAGTTTTTCTTATGTATTTTTCTTTAGTTAATCGTTTTTTCTTATGTTTCCGAAAGTAGTGGTGGATGATTTAAGTTTTATATCTCTCACTAAATGTTATTATCACAGATCTTTAGAGCATTGTCATATATCCACCGTTGTATTGGGGTGTGCCACCGGGACATTAAACCTCAAAATCTTTTGGTAAGTTGAGAAATGCAATAATTAGGGCCCTCATTATTTTCTCCTGACACCCATATACATAATTTGTTTCTCCTTTCTATCAATTTATGCAGGTGAACCCGCATACTCACCAGGTTAAACTATGTGATTTTGGGAGTGCAAAAGTCTTGGTATGTTCTTCTTTTTCTCATAATAAAAGGTCATTTGGGCTGATTGTTGTCTGACCAAGTAGATGCGATTATATACTTTGTGGTGTCAGGTAAAAGGGGAGCCAAATATATCCTATATCTGTTCCAGATACTATAGAGCTCCTGAGCTTATTTTTGGAGCAACAGAGTATACTACGGCAATTGATATATGGTCTGCAGGTTGTGTTCTTGCTGAGCTATTGCTTGGTCAGGTATTTGGTTGTATGTGTTTTCTATTTTGCATGTTTTTTAGTATGAGATTTTATGGAAACTAAAGATTTATTTATTTATTTATTTATTTTGTATCAGCCTCTTTTTCCTGGAGAGAGTGGAGTTGACCAGCTTGTTGAGATCATTAAGGTAGCTTTGACATTTCAAATTTCTGAGTAAATATTGCAAATCATGCTCCTAACACTTCCCAATTATGTTCACAGTGTTAAGCTGTCTTTTTGACATGATTTAGTGTTGAAAATCCAAAGAATCAGTCAAGATTTTGATGACTGATTATTATAAAGTAGATTCACTGTTAGAATTTTTTGAACCTGAAGCTGCTTATCACATTTCTCTTTTTTTAAAAAAAAAAAAATAGGAGAAAGTAAATTTACTGTTGTGTTTTAAGTATTTAATGTTTATCTCTCCATTGATTTGTATTGGCTGCTTAAATCTTCGGACTTTTCTTGGATGACAAAAACCCACTTATTTAATATTGCAATCCTGAGGATGTTTACCATTTTCCTTCCTACGCTTTTTACTTTATCCCTAAGTGTTATTTTAAGCTTTATGTACTTGTATGTAATAGAATTGGTTTTGTAGGTTTTGGGCACTCCTACCAGGGAAGAAATTAAATGCATGAATCCCAATTATACAGAGTATAAATTCCCTCAAATTAAAGCCCACCCATGGCACAAGGTAATTGTATATGGAAATATTGTATGTATATGTATTTATTTATTACTTTTTCTGAACTATGTATTTAGGCATTGACTTATCATGCAGATATTTCACAAACGTATGCCACCGGAAGCTGTCGATCTTGTGTCTAGACTATTGCAATATTCTCCTAACCTAAGGTGCACAGCGGTAAGTATCTGGTCTCACAGCAGACTTGCCATGCTAATACATAACATCTATATTTTGTATTTGGGCTGTTCAGATGAAATGAAAATTAGGTATACTGGCTTGGCCATTATCTTGATCTACTTTAAAATTCTGATGGTAGTGTGTCTTTTCTCCTCATCGTCTTCTGTCACCAACTTCTGCAACATTTTATTTTCTTTTAATACGTTTATATATTAGTGGTTCCTAACCTTGGCCTATTTTAGCATTTTACTTGCTCACTTATGTTGTATTTGAAAATGCTATGTCATTGATTGAAGAAGACACTCTTTCAATGTAAAAGAAATAGGTGGCTACCAAAAAGTGATCTATGGGTTGTCTTGTTGAGCAATATGGATATTTCCACCTAATAAAAAGGACCTCTGAAATTCAGTCTGATATCATCAATCTTTTGACTTTTAGCCCTTTGGGGCAGCATGGTTGTTGACTTGTCGTCTTCATGTGCAAGACATCTACCTGTTCTCATATTCTTATATCTCCATTTTGTTATAATAAGAGTTTCTCAATTGTAATATTTGAATTAATTAATTCCATTTGACAATGATACAGTTGGATGCCTTGACTCATCAGTTTTTTGATGAGCTTCGTGATCCGAATACCCGATTGCCTAATGGACGCTTCCTTCCACCACTATTCAACTTTAAAGCTCATGGTATGGTCACATATGATATATTTTTATAGAAAAAATAAGTGCAAACTATTTTCATCCAACTTATTGTTCTATTTGACATGCTAATTAATCTTTTGCATTTAATGTTGTGGCTACTGCTGCCTGCTGGTTCATTTGACTAGAATTGAAGGGTGTGCCTGTTGAGATATTGGTGAAATTGATACCAGAACATGCAAGAAAACAGTGTCCATTTCTTGGGTTATGAATTTGATTAGAAACATAACCGTTTCACCGGTGGTATTGTCTTGACAAAGAAAGCTACGGGTGTCCCAGTCTGTGCTATTCCTTCTGTTTGCTACGAGTATATCTCTGTATTACCTACTACCTTGTTTTGAAAGAGCAGATCTAGATATGTTCTTAATGAATGGGTAACAACTCTATCTAATGTTCCATGTGTATTGAATTCGGGTTCAAGTGTTTTAGTTTGTATTAGGATTTGAATGTAGTTAAGGTGAAGTTGGCAGCGGATTTCTTGGTCCGGCTGCCCTCGACGGAACTGGGTTACCTGGCTACACAGTCTAG

mRNA sequence

AGGACACCTATTTTTATTTTGCTGGGCTCTTCTTTTTCTTTGACTGTCACCAGCGCATTATAGCATATACCACAGTCCACAACAAATACAATACAAATAAAGATTAAAAAAAAAAAAAAAAACTCTCTCTTTCCATTCCTTTCCTTCTCTCTCTCCTAACGCAAAATCCAAAAGGGAAAACCCAGTTCTTCACATCGCACACCCTACGAAACTTTCTCGCGGATCTGACCCTTTAATTTTCTCCCACCAACCTGCCTCTCTGTTTTCTCCTCCATGTCTCAAGATTTTACATTCCATCGGTGAATGGTTAGTTTTATAAGGATCATTCACTGAAGAAAGCTCGTGACGATTTCAAGAAATGGCTTCTATGGGTGTTGTTTCTACTTCAAGTTTGAGAGAGCCAGCTGGTCATAATGTTGGTGTTGATAGGTTACCCGAAGAGATGAATGACATGAAAATTAGAGATGACAAGACAATCAGCTACATGGCAGAACGTGCTGTTGGGCATGGATCATTTGGAGTCGTGTTTCAAGCAAAGTGCTTAGAGACTGGGGAAACTGTGGCTATAAAGAAGGTTCTCCAAGACAAAAGGTACAAGAACCGTGAGCTGCAAACTATGCGGCTTCTTGACCATCCAAATGTTGTTGCATTAAAGCATTGCTTCTTCTCAACCACCGAAAAGGATGAACTATATCTCAACCTGGTTCTTGAGTATGTCCCTGAAACAGTTCATAGGGTGATAAAGCACTACAACAAGTTGAATCAAAGGATGCCACTGATCTATGTAAAACTTTACATGTACCAGATCTTTAGAGCATTGTCATATATCCACCGTTGTATTGGGGTGTGCCACCGGGACATTAAACCTCAAAATCTTTTGGTGAACCCGCATACTCACCAGGTTAAACTATGTGATTTTGGGAGTGCAAAAGTCTTGGTAAAAGGGGAGCCAAATATATCCTATATCTGTTCCAGATACTATAGAGCTCCTGAGCTTATTTTTGGAGCAACAGAGTATACTACGGCAATTGATATATGGTCTGCAGGTTGTGTTCTTGCTGAGCTATTGCTTGGTCAGCCTCTTTTTCCTGGAGAGAGTGGAGTTGACCAGCTTGTTGAGATCATTAAGGTTTTGGGCACTCCTACCAGGGAAGAAATTAAATGCATGAATCCCAATTATACAGAGTATAAATTCCCTCAAATTAAAGCCCACCCATGGCACAAGATATTTCACAAACGTATGCCACCGGAAGCTGTCGATCTTGTGTCTAGACTATTGCAATATTCTCCTAACCTAAGGTGCACAGCGTTGGATGCCTTGACTCATCAGTTTTTTGATGAGCTTCGTGATCCGAATACCCGATTGCCTAATGGACGCTTCCTTCCACCACTATTCAACTTTAAAGCTCATGGTGAAGTTGGCAGCGGATTTCTTGGTCCGGCTGCCCTCGACGGAACTGGGTTACCTGGCTACACAGTCTAG

Coding sequence (CDS)

ATGGCTTCTATGGGTGTTGTTTCTACTTCAAGTTTGAGAGAGCCAGCTGGTCATAATGTTGGTGTTGATAGGTTACCCGAAGAGATGAATGACATGAAAATTAGAGATGACAAGACAATCAGCTACATGGCAGAACGTGCTGTTGGGCATGGATCATTTGGAGTCGTGTTTCAAGCAAAGTGCTTAGAGACTGGGGAAACTGTGGCTATAAAGAAGGTTCTCCAAGACAAAAGGTACAAGAACCGTGAGCTGCAAACTATGCGGCTTCTTGACCATCCAAATGTTGTTGCATTAAAGCATTGCTTCTTCTCAACCACCGAAAAGGATGAACTATATCTCAACCTGGTTCTTGAGTATGTCCCTGAAACAGTTCATAGGGTGATAAAGCACTACAACAAGTTGAATCAAAGGATGCCACTGATCTATGTAAAACTTTACATGTACCAGATCTTTAGAGCATTGTCATATATCCACCGTTGTATTGGGGTGTGCCACCGGGACATTAAACCTCAAAATCTTTTGGTGAACCCGCATACTCACCAGGTTAAACTATGTGATTTTGGGAGTGCAAAAGTCTTGGTAAAAGGGGAGCCAAATATATCCTATATCTGTTCCAGATACTATAGAGCTCCTGAGCTTATTTTTGGAGCAACAGAGTATACTACGGCAATTGATATATGGTCTGCAGGTTGTGTTCTTGCTGAGCTATTGCTTGGTCAGCCTCTTTTTCCTGGAGAGAGTGGAGTTGACCAGCTTGTTGAGATCATTAAGGTTTTGGGCACTCCTACCAGGGAAGAAATTAAATGCATGAATCCCAATTATACAGAGTATAAATTCCCTCAAATTAAAGCCCACCCATGGCACAAGATATTTCACAAACGTATGCCACCGGAAGCTGTCGATCTTGTGTCTAGACTATTGCAATATTCTCCTAACCTAAGGTGCACAGCGTTGGATGCCTTGACTCATCAGTTTTTTGATGAGCTTCGTGATCCGAATACCCGATTGCCTAATGGACGCTTCCTTCCACCACTATTCAACTTTAAAGCTCATGGTGAAGTTGGCAGCGGATTTCTTGGTCCGGCTGCCCTCGACGGAACTGGGTTACCTGGCTACACAGTCTAG

Protein sequence

MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDKTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDELRDPNTRLPNGRFLPPLFNFKAHGEVGSGFLGPAALDGTGLPGYTV
Homology
BLAST of Lsi06G000310 vs. ExPASy Swiss-Prot
Match: Q40518 (Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 8.0e-189
Identity = 325/382 (85.08%), Postives = 334/382 (87.43%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           M S+G+   S LRE + H+VGVDRLPEEMNDM+IRDDK                      
Sbjct: 1   MTSVGLAPVSGLRESSSHSVGVDRLPEEMNDMRIRDDKEIEAAIVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAER VG GSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRHGQPKQTISYMAERIVGQGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVV LKHCFFSTTEKDE+YLNLVLEYVPETVHRVIKHYNKLNQRMPLI VKLY YQ
Sbjct: 121 LDHPNVVCLKHCFFSTTEKDEVYLNLVLEYVPETVHRVIKHYNKLNQRMPLILVKLYTYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIH  IGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHHTIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNY E+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTAL+A+TH FFDEL
Sbjct: 301 MNPNYNEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEAVTHAFFDEL 360

BLAST of Lsi06G000310 vs. ExPASy Swiss-Prot
Match: P43289 (Shaggy-related protein kinase gamma OS=Arabidopsis thaliana OX=3702 GN=ASK3 PE=1 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 2.3e-188
Identity = 320/382 (83.77%), Postives = 335/382 (87.70%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MAS+G+  ++++RE  G+    DRLPEEM DMKI+DDK                      
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFR+LSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTE+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRC ALD+L H FFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCAALDSLVHPFFDEL 360

BLAST of Lsi06G000310 vs. ExPASy Swiss-Prot
Match: P43288 (Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1 SV=3)

HSP 1 Score: 654.4 bits (1687), Expect = 7.5e-187
Identity = 322/382 (84.29%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MAS+G+      R+    + GVD+LPEEMNDMKIRDDK                      
Sbjct: 1   MASVGIAPNPGARD----STGVDKLPEEMNDMKIRDDKEMEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTE+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR  ALD L H FFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVHPFFDEL 360

BLAST of Lsi06G000310 vs. ExPASy Swiss-Prot
Match: P51137 (Glycogen synthase kinase-3 homolog MsK-1 OS=Medicago sativa OX=3879 GN=MSK-1 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 4.1e-185
Identity = 322/384 (83.85%), Postives = 331/384 (86.20%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGH-NVGVDR-LPEEMNDMKIRDDK-------------------- 60
           MAS+GV  TS  RE  G   +GVD  LPEEM+DMKIRDD+                    
Sbjct: 1   MASVGVAPTSGFREVLGDGEIGVDDILPEEMSDMKIRDDREMEATVVDGNGTETGHIIVT 60

Query: 61  -----------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTM 120
                      TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTM
Sbjct: 61  TIGGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTM 120

Query: 121 RLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYM 180
           RLLDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHY+KLNQRMP+IYVKLY 
Sbjct: 121 RLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYSKLNQRMPMIYVKLYT 180

Query: 181 YQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRY 240
           YQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRY
Sbjct: 181 YQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRY 240

Query: 241 YRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEI 300
           YRAPELIFGATEYTTAID+WS GCVLAELLLGQPLFPGE GVDQLVEIIKVLGTPTREEI
Sbjct: 241 YRAPELIFGATEYTTAIDVWSVGCVLAELLLGQPLFPGERGVDQLVEIIKVLGTPTREEI 300

Query: 301 KCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFD 352
           KCMNPNYTE+KFPQIKAHPWHKIFHKRMP EAVDLVSRLLQYSPNLRC ALD LTH FFD
Sbjct: 301 KCMNPNYTEFKFPQIKAHPWHKIFHKRMPAEAVDLVSRLLQYSPNLRCQALDCLTHPFFD 360

BLAST of Lsi06G000310 vs. ExPASy Swiss-Prot
Match: P51139 (Glycogen synthase kinase-3 homolog MsK-3 OS=Medicago sativa OX=3879 GN=MSK-3 PE=2 SV=2)

HSP 1 Score: 636.3 bits (1640), Expect = 2.1e-181
Identity = 312/383 (81.46%), Postives = 329/383 (85.90%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MAS GV   S   +    +VGV++LPEEMNDMKIRDDK                      
Sbjct: 1   MASGGVAPASGFIDKNASSVGVEKLPEEMNDMKIRDDKEMEAATIVDGNGTETGHIIVTT 60

Query: 61  ----------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
                     TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGKNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMY 180
           LLDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETV RVI+HYNK+NQRMP+IYVKLY Y
Sbjct: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVSRVIRHYNKMNQRMPMIYVKLYSY 180

Query: 181 QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240
           QI RAL+YIH  IGVCHRDIKPQNLLVNPHTHQ+K+CDFGSAKVLVKGEPNISYICSRYY
Sbjct: 181 QICRALAYIHNSIGVCHRDIKPQNLLVNPHTHQLKICDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYTTAIDIWSAGCVL ELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTTAIDIWSAGCVLGELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE 352
           CMNPNYTE+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR TAL+AL H F+D+
Sbjct: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALVHPFYDD 360

BLAST of Lsi06G000310 vs. ExPASy TrEMBL
Match: A0A5A7UBP7 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004590 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.4e-212
Identity = 372/422 (88.15%), Postives = 373/422 (88.39%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVVSTS+LREPAGHNVGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVSTSNLREPAGHNVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  --------------------------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKK 120
                                     TISYMAERAVGHGSFGVVFQAKCLETGETVAIKK
Sbjct: 61  GVWAFFLLSSSYFFVMPLMVCGITLQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKK 120

Query: 121 VLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYN 180
           VLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYN
Sbjct: 121 VLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYN 180

Query: 181 KLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKV 240
           KLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKV
Sbjct: 181 KLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKV 240

Query: 241 LVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQL 300
           LVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQL
Sbjct: 241 LVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQL 300

Query: 301 VEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPN 360
           VEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPN
Sbjct: 301 VEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPN 360

Query: 361 LRCTALDALTHQFFDELRDPNTRLPNGRFLPPLFNFKAHGEVGSGFLGPAALDGTGLPGY 375
           LRCTALDALTHQFFDELRDPNTRLPNGR LPPLFNFKAHGEVGSGFLGPAALDGTGLPGY
Sbjct: 361 LRCTALDALTHQFFDELRDPNTRLPNGRILPPLFNFKAHGEVGSGFLGPAALDGTGLPGY 420

BLAST of Lsi06G000310 vs. ExPASy TrEMBL
Match: A0A1S3BPD1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103492102 PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.6e-200
Identity = 349/382 (91.36%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVVSTS+LREPAGHNVGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVSTSNLREPAGHNVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRNGYSKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ
Sbjct: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL
Sbjct: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 360

BLAST of Lsi06G000310 vs. ExPASy TrEMBL
Match: A0A6J1DUK6 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111024174 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.2e-200
Identity = 348/382 (91.10%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVV TSSLREPAGH+VGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVPTSSLREPAGHSVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRNGYPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ
Sbjct: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRC+ALDALTHQFFDEL
Sbjct: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCSALDALTHQFFDEL 360

BLAST of Lsi06G000310 vs. ExPASy TrEMBL
Match: A0A6J1JP89 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111486470 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.2e-199
Identity = 348/383 (90.86%), Postives = 350/383 (91.38%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGH-NVGVDRLPEEMNDMKIRDDK--------------------- 60
           M SMGVVSTSSLREPAGH NVGVDRLPEE+NDMKIRDDK                     
Sbjct: 1   MGSMGVVSTSSLREPAGHNNVGVDRLPEELNDMKIRDDKEVEATIVDGNGTETGHIIVTT 60

Query: 61  ----------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
                     TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGRNGYPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMY 180
           LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMP+IYVKLYMY
Sbjct: 121 LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPVIYVKLYMY 180

Query: 181 QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240
           QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY
Sbjct: 181 QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE 352
           CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE
Sbjct: 301 CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE 360

BLAST of Lsi06G000310 vs. ExPASy TrEMBL
Match: A0A6J1EU30 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111437759 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.2e-199
Identity = 348/383 (90.86%), Postives = 350/383 (91.38%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGH-NVGVDRLPEEMNDMKIRDDK--------------------- 60
           M SMGVVSTSSLREPAGH NVGVDRLPEE+NDMKIRDDK                     
Sbjct: 1   MGSMGVVSTSSLREPAGHNNVGVDRLPEELNDMKIRDDKEVEATVVDGNGTETGHIIVTT 60

Query: 61  ----------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
                     TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGRNGYPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMY 180
           LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMP+IYVKLYMY
Sbjct: 121 LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPVIYVKLYMY 180

Query: 181 QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240
           QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY
Sbjct: 181 QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE 352
           CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE
Sbjct: 301 CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE 360

BLAST of Lsi06G000310 vs. NCBI nr
Match: KAA0050959.1 (shaggy-related protein kinase NtK-1 [Cucumis melo var. makuwa] >TYK10306.1 shaggy-related protein kinase NtK-1 [Cucumis melo var. makuwa])

HSP 1 Score: 748.4 bits (1931), Expect = 2.9e-212
Identity = 372/422 (88.15%), Postives = 373/422 (88.39%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVVSTS+LREPAGHNVGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVSTSNLREPAGHNVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  --------------------------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKK 120
                                     TISYMAERAVGHGSFGVVFQAKCLETGETVAIKK
Sbjct: 61  GVWAFFLLSSSYFFVMPLMVCGITLQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKK 120

Query: 121 VLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYN 180
           VLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYN
Sbjct: 121 VLQDKRYKNRELQTMRLLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYN 180

Query: 181 KLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKV 240
           KLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKV
Sbjct: 181 KLNQRMPLIYVKLYMYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKV 240

Query: 241 LVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQL 300
           LVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQL
Sbjct: 241 LVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQL 300

Query: 301 VEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPN 360
           VEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPN
Sbjct: 301 VEIIKVLGTPTREEIKCMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPN 360

Query: 361 LRCTALDALTHQFFDELRDPNTRLPNGRFLPPLFNFKAHGEVGSGFLGPAALDGTGLPGY 375
           LRCTALDALTHQFFDELRDPNTRLPNGR LPPLFNFKAHGEVGSGFLGPAALDGTGLPGY
Sbjct: 361 LRCTALDALTHQFFDELRDPNTRLPNGRILPPLFNFKAHGEVGSGFLGPAALDGTGLPGY 420

BLAST of Lsi06G000310 vs. NCBI nr
Match: XP_038878992.1 (shaggy-related protein kinase alpha [Benincasa hispida])

HSP 1 Score: 710.7 bits (1833), Expect = 6.7e-201
Identity = 350/382 (91.62%), Postives = 351/382 (91.88%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVVSTSSLREPAGHNVGVD+LPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVSTSSLREPAGHNVGVDKLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRNGYSKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ
Sbjct: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL
Sbjct: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 360

BLAST of Lsi06G000310 vs. NCBI nr
Match: XP_004135570.1 (shaggy-related protein kinase alpha [Cucumis sativus] >XP_031745302.1 shaggy-related protein kinase alpha [Cucumis sativus] >KAE8653337.1 hypothetical protein Csa_007159 [Cucumis sativus])

HSP 1 Score: 709.1 bits (1829), Expect = 2.0e-200
Identity = 349/382 (91.36%), Postives = 351/382 (91.88%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVVSTS+LREPAGHNVGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVSTSNLREPAGHNVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRNGYSKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ
Sbjct: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRC+ALDALTHQFFDEL
Sbjct: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCSALDALTHQFFDEL 360

BLAST of Lsi06G000310 vs. NCBI nr
Match: XP_008450520.1 (PREDICTED: shaggy-related protein kinase NtK-1 [Cucumis melo])

HSP 1 Score: 708.4 bits (1827), Expect = 3.3e-200
Identity = 349/382 (91.36%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVVSTS+LREPAGHNVGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVSTSNLREPAGHNVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRNGYSKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ
Sbjct: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL
Sbjct: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 360

BLAST of Lsi06G000310 vs. NCBI nr
Match: XP_022157482.1 (shaggy-related protein kinase alpha [Momordica charantia])

HSP 1 Score: 706.4 bits (1822), Expect = 1.3e-199
Identity = 348/382 (91.10%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MASMGVV TSSLREPAGH+VGVDRLPEEMNDMKIRDDK                      
Sbjct: 1   MASMGVVPTSSLREPAGHSVGVDRLPEEMNDMKIRDDKEVEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRNGYPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ
Sbjct: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRC+ALDALTHQFFDEL
Sbjct: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCSALDALTHQFFDEL 360

BLAST of Lsi06G000310 vs. TAIR 10
Match: AT3G05840.2 (Protein kinase superfamily protein )

HSP 1 Score: 659.4 bits (1700), Expect = 1.7e-189
Identity = 320/382 (83.77%), Postives = 335/382 (87.70%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MAS+G+  ++++RE  G+    DRLPEEM DMKI+DDK                      
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFR+LSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTE+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRC ALD+L H FFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCAALDSLVHPFFDEL 360

BLAST of Lsi06G000310 vs. TAIR 10
Match: AT3G05840.1 (Protein kinase superfamily protein )

HSP 1 Score: 659.4 bits (1700), Expect = 1.7e-189
Identity = 320/382 (83.77%), Postives = 335/382 (87.70%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MAS+G+  ++++RE  G+    DRLPEEM DMKI+DDK                      
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFR+LSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTE+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRC ALD+L H FFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCAALDSLVHPFFDEL 360

BLAST of Lsi06G000310 vs. TAIR 10
Match: AT5G26751.1 (shaggy-related kinase 11 )

HSP 1 Score: 654.4 bits (1687), Expect = 5.3e-188
Identity = 322/382 (84.29%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 1   MASMGVVSTSSLREPAGHNVGVDRLPEEMNDMKIRDDK---------------------- 60
           MAS+G+      R+    + GVD+LPEEMNDMKIRDDK                      
Sbjct: 1   MASVGIAPNPGARD----STGVDKLPEEMNDMKIRDDKEMEATVVDGNGTETGHIIVTTI 60

Query: 61  ---------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
                    TISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQ 180
           LDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQ 180

Query: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240
           IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTTAID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDEL 352
           MNPNYTE+KFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR  ALD L H FFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVHPFFDEL 360

BLAST of Lsi06G000310 vs. TAIR 10
Match: AT5G14640.1 (shaggy-like kinase 13 )

HSP 1 Score: 611.3 bits (1575), Expect = 5.2e-175
Identity = 301/381 (79.00%), Postives = 325/381 (85.30%), Query Frame = 0

Query: 1   MASMGVVSTSSL-REPAGHNVGVDRLPEEMNDMKIRDDK--------------------- 60
           MAS+G +  SS+  + +  ++  ++LPE +N+MKI+DDK                     
Sbjct: 1   MASVGTLPASSMATKQSNASICAEKLPEGINEMKIKDDKEMEAAVVDGNGTETGHIIVTT 60

Query: 61  ----------TISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
                     TISYMAER VG GSFG+VFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGKNGQPKQTISYMAERIVGQGSFGIVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMY 180
           LLDHPNVV+LKHCFFSTTEKDELYLNLVLEYVPETV+RV KHY++ NQRMP+IYVKLY Y
Sbjct: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVYRVSKHYSRANQRMPIIYVKLYTY 180

Query: 181 QIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240
           QI RAL+YIH  +GVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY
Sbjct: 181 QICRALAYIHGGVGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYTT IDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTTTIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEYKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDE 350
           CMNPNYTE+KFPQIKAHPWHKIFHKR PPEAVDLVSRLLQYSPNLR TA++A+ H FFDE
Sbjct: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRTPPEAVDLVSRLLQYSPNLRSTAMEAIVHPFFDE 360

BLAST of Lsi06G000310 vs. TAIR 10
Match: AT4G00720.1 (shaggy-like protein kinase 32 )

HSP 1 Score: 570.5 bits (1469), Expect = 1.0e-162
Identity = 281/389 (72.24%), Postives = 309/389 (79.43%), Query Frame = 0

Query: 23  DRLPEEMNDMKIRDDK--------------------------------------TISYMA 82
           D+LP+ M +MKIRD++                                      TISYMA
Sbjct: 81  DQLPDVMIEMKIRDERNANREDKDMETTVVNGSGTETGQVITTTVGGRDGKPKQTISYMA 140

Query: 83  ERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVALKHCFFS 142
           +R VG GSFGVVFQAKCLETGE VAIKKVLQDKRYKNRELQ MRL DHPNVV L+H FFS
Sbjct: 141 QRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLRHSFFS 200

Query: 143 TTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYMYQIFRALSYIHRCIGVC 202
           TT+KDELYLNLVLEYVPETV+R  KHY K+NQ MP+I+V+LY YQI RAL+Y+HR +GVC
Sbjct: 201 TTDKDELYLNLVLEYVPETVYRASKHYTKMNQHMPIIFVQLYTYQICRALNYLHRVVGVC 260

Query: 203 HRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAI 262
           HRDIKPQNLLVNP THQ+K+CDFGSAK+LV GEPNISYICSRYYRAPELIFGATEYT AI
Sbjct: 261 HRDIKPQNLLVNPQTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEYTNAI 320

Query: 263 DIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEYKFPQIKA 322
           D+WS GCV+AELLLGQPLFPGESG+DQLVEIIK+LGTPTREEI+CMNPNYTE+KFPQIKA
Sbjct: 321 DMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKFPQIKA 380

Query: 323 HPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALDALTHQFFDELRDPNTRLPNGRFLPP 369
           HPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTAL+A  H FFD+LRDPN  LPNGR LPP
Sbjct: 381 HPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNVSLPNGRALPP 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q405188.0e-18985.08Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 S... [more]
P432892.3e-18883.77Shaggy-related protein kinase gamma OS=Arabidopsis thaliana OX=3702 GN=ASK3 PE=1... [more]
P432887.5e-18784.29Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1... [more]
P511374.1e-18583.85Glycogen synthase kinase-3 homolog MsK-1 OS=Medicago sativa OX=3879 GN=MSK-1 PE=... [more]
P511392.1e-18181.46Glycogen synthase kinase-3 homolog MsK-3 OS=Medicago sativa OX=3879 GN=MSK-3 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7UBP71.4e-21288.15Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BPD11.6e-20091.36Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1DUK66.2e-20091.10Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1JP895.2e-19990.86Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EU305.2e-19990.86Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
KAA0050959.12.9e-21288.15shaggy-related protein kinase NtK-1 [Cucumis melo var. makuwa] >TYK10306.1 shagg... [more]
XP_038878992.16.7e-20191.62shaggy-related protein kinase alpha [Benincasa hispida][more]
XP_004135570.12.0e-20091.36shaggy-related protein kinase alpha [Cucumis sativus] >XP_031745302.1 shaggy-rel... [more]
XP_008450520.13.3e-20091.36PREDICTED: shaggy-related protein kinase NtK-1 [Cucumis melo][more]
XP_022157482.11.3e-19991.10shaggy-related protein kinase alpha [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G05840.21.7e-18983.77Protein kinase superfamily protein [more]
AT3G05840.11.7e-18983.77Protein kinase superfamily protein [more]
AT5G26751.15.3e-18884.29shaggy-related kinase 11 [more]
AT5G14640.15.2e-17579.00shaggy-like kinase 13 [more]
AT4G00720.11.0e-16272.24shaggy-like protein kinase 32 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 42..326
e-value: 3.4E-90
score: 315.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 42..326
e-value: 3.8E-65
score: 219.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 42..326
score: 43.594215
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 28..121
e-value: 4.7E-35
score: 121.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 122..355
e-value: 2.4E-81
score: 274.7
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 42..254
e-value: 1.4E-21
score: 74.4
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 34..241
e-value: 4.1E-16
score: 56.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR24057GLYCOGEN SYNTHASE KINASE-3 ALPHAcoord: 38..352
NoneNo IPR availablePANTHERPTHR24057:SF65SHAGGY-RELATED PROTEIN KINASE ALPHAcoord: 38..352
NoneNo IPR availablePANTHERPTHR24057:SF65SHAGGY-RELATED PROTEIN KINASE ALPHAcoord: 1..39
NoneNo IPR availablePANTHERPTHR24057GLYCOGEN SYNTHASE KINASE-3 ALPHAcoord: 1..39
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 163..175
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 48..72
IPR039192Glycogen synthase kinase 3, catalytic domainCDDcd14137STKc_GSK3coord: 38..329
e-value: 0.0
score: 602.185
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 41..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G000310.1Lsi06G000310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004672 protein kinase activity