Lsi05G021540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G021540
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAAA-ATPase
Locationchr05: 28261653 .. 28263098 (-)
RNA-Seq ExpressionLsi05G021540
SyntenyLsi05G021540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTCTTTTTCAGGTCCTTTTCTGTAATCTTCATCGGAATTTGTTTCTCTTGGTTGTTGCGGATTCTTTTGTTCAGGACGGGATTGATTTTCGTTGTCAACAAATGGTGGAGAAACCTAGAAGATTGTTTCCATGTTTACCAATCTTTCAGAATCCCGGAATTCAATGAAACTTCCCAACAGAATCACCTTTACCGGAAAGTCTCTGCTTATCTTACTTCTTTATCCTCTCTCGAGGATTCCGACTTCACCAATCTCATCACCGGAAACAAGCCTAATGACATCATTCTCCGGCTCGATTCCAATCAGACAGTTCAGGACAATTTTTTGGGGGCGAAAGTGTTTTGGACGAATGAACAAAACAGTTCCAGAAATTTTGTGTTGAAGATTAGAAAGGCTGATAAGCGCCGAATCCTCCGCCCTTATCTTCAGCACATTCATACCCTAACCGCTGATGAGATTGAGCAGAGGAAAGGGGATTTGAAGCTTTTTATGAATTCAAAACCTAATAATCAGTCAGATACAAGATGGAAATCGATTCAATTCAAACATCCATCTACTTTCGATTCGATTGCTATGGAAACAGATCTCAAAGAAAAGGTCAAATCCGATCTCGAATCGTTTCTCAAATCCAAACAATATTATCACAGATTAGGCAGAGTCTGGAAACGGAGTTACCTTCTATACGGCCCTTCCGGAACTGGAAAATCGAGCTTCGTCGCCGCTATGGCGAATTTCCTCTCCTATGATGTATACGATATCGACCTCTTCAAAGTCTCCGATGACTCTGATCTCAAGTTTCTTCTGCTACAGACCACAGGCAAGTCAATAATCGTCGTCGAGGATCTCGATCGGTTTCTCACCGAGAAATCGTCGGCGTTGAGCCTATCAGCGCTGCTGAACTTCATGGACGGAATATTAACATCGTGCTGTGCGGAAGAGAGAGTAATGGTTTTCACCGTCAATTGCAAGGAACAAGTCGAACCGGCGATTCTCCGACCGGGCCGTATCGACGTTCATATCCATTTTCCACTCTGCGATTTCTCTGCTTTCAAGAATCTCGCAATCAATTATCTAGGCGTCAAGGATCACAAATTGTTCCCTCAGGTTGAAGAAATATTCCAAACCGGCGCCAGTTTGAGCCCCGCCGAGATCGGCGAACTGATGATCGCGAACCGAAACTCGCCTAGCCGAGCGATTAAATCGGTCATCTCAGCGTTGCAAACCGACGGCGACCGGCGGAGAGTCGGCAACGTCGTCCGGCGGCTGAGCGACTGCGGGTCGAGGAAGTCCGTCGCCGAATCCGTCGAATCGGGCGGCGTATTATGCAGCGAAAACACTCAGACCGGAAAAGAGTTCAGAAAATTGTACGGGTTTCTAAGGATGAAAAGTAACAAAATATCTCAGTCGTTCGATTCGTCTCCTATGCACAAAGAGAGTTGA

mRNA sequence

ATGGAGTTCTTTTTCAGGTCCTTTTCTGTAATCTTCATCGGAATTTGTTTCTCTTGGTTGTTGCGGATTCTTTTGTTCAGGACGGGATTGATTTTCGTTGTCAACAAATGGTGGAGAAACCTAGAAGATTGTTTCCATGTTTACCAATCTTTCAGAATCCCGGAATTCAATGAAACTTCCCAACAGAATCACCTTTACCGGAAAGTCTCTGCTTATCTTACTTCTTTATCCTCTCTCGAGGATTCCGACTTCACCAATCTCATCACCGGAAACAAGCCTAATGACATCATTCTCCGGCTCGATTCCAATCAGACAGTTCAGGACAATTTTTTGGGGGCGAAAGTGTTTTGGACGAATGAACAAAACAGTTCCAGAAATTTTGTGTTGAAGATTAGAAAGGCTGATAAGCGCCGAATCCTCCGCCCTTATCTTCAGCACATTCATACCCTAACCGCTGATGAGATTGAGCAGAGGAAAGGGGATTTGAAGCTTTTTATGAATTCAAAACCTAATAATCAGTCAGATACAAGATGGAAATCGATTCAATTCAAACATCCATCTACTTTCGATTCGATTGCTATGGAAACAGATCTCAAAGAAAAGGTCAAATCCGATCTCGAATCGTTTCTCAAATCCAAACAATATTATCACAGATTAGGCAGAGTCTGGAAACGGAGTTACCTTCTATACGGCCCTTCCGGAACTGGAAAATCGAGCTTCGTCGCCGCTATGGCGAATTTCCTCTCCTATGATGTATACGATATCGACCTCTTCAAAGTCTCCGATGACTCTGATCTCAAGTTTCTTCTGCTACAGACCACAGGCAAGTCAATAATCGTCGTCGAGGATCTCGATCGGTTTCTCACCGAGAAATCGTCGGCGTTGAGCCTATCAGCGCTGCTGAACTTCATGGACGGAATATTAACATCGTGCTGTGCGGAAGAGAGAGTAATGGTTTTCACCGTCAATTGCAAGGAACAAGTCGAACCGGCGATTCTCCGACCGGGCCGTATCGACGTTCATATCCATTTTCCACTCTGCGATTTCTCTGCTTTCAAGAATCTCGCAATCAATTATCTAGGCGTCAAGGATCACAAATTGTTCCCTCAGGTTGAAGAAATATTCCAAACCGGCGCCAGTTTGAGCCCCGCCGAGATCGGCGAACTGATGATCGCGAACCGAAACTCGCCTAGCCGAGCGATTAAATCGGTCATCTCAGCGTTGCAAACCGACGGCGACCGGCGGAGAGTCGGCAACGTCGTCCGGCGGCTGAGCGACTGCGGGTCGAGGAAGTCCGTCGCCGAATCCGTCGAATCGGGCGGCGTATTATGCAGCGAAAACACTCAGACCGGAAAAGAGTTCAGAAAATTGTACGGGTTTCTAAGGATGAAAAGTAACAAAATATCTCAGTCGTTCGATTCGTCTCCTATGCACAAAGAGAGTTGA

Coding sequence (CDS)

ATGGAGTTCTTTTTCAGGTCCTTTTCTGTAATCTTCATCGGAATTTGTTTCTCTTGGTTGTTGCGGATTCTTTTGTTCAGGACGGGATTGATTTTCGTTGTCAACAAATGGTGGAGAAACCTAGAAGATTGTTTCCATGTTTACCAATCTTTCAGAATCCCGGAATTCAATGAAACTTCCCAACAGAATCACCTTTACCGGAAAGTCTCTGCTTATCTTACTTCTTTATCCTCTCTCGAGGATTCCGACTTCACCAATCTCATCACCGGAAACAAGCCTAATGACATCATTCTCCGGCTCGATTCCAATCAGACAGTTCAGGACAATTTTTTGGGGGCGAAAGTGTTTTGGACGAATGAACAAAACAGTTCCAGAAATTTTGTGTTGAAGATTAGAAAGGCTGATAAGCGCCGAATCCTCCGCCCTTATCTTCAGCACATTCATACCCTAACCGCTGATGAGATTGAGCAGAGGAAAGGGGATTTGAAGCTTTTTATGAATTCAAAACCTAATAATCAGTCAGATACAAGATGGAAATCGATTCAATTCAAACATCCATCTACTTTCGATTCGATTGCTATGGAAACAGATCTCAAAGAAAAGGTCAAATCCGATCTCGAATCGTTTCTCAAATCCAAACAATATTATCACAGATTAGGCAGAGTCTGGAAACGGAGTTACCTTCTATACGGCCCTTCCGGAACTGGAAAATCGAGCTTCGTCGCCGCTATGGCGAATTTCCTCTCCTATGATGTATACGATATCGACCTCTTCAAAGTCTCCGATGACTCTGATCTCAAGTTTCTTCTGCTACAGACCACAGGCAAGTCAATAATCGTCGTCGAGGATCTCGATCGGTTTCTCACCGAGAAATCGTCGGCGTTGAGCCTATCAGCGCTGCTGAACTTCATGGACGGAATATTAACATCGTGCTGTGCGGAAGAGAGAGTAATGGTTTTCACCGTCAATTGCAAGGAACAAGTCGAACCGGCGATTCTCCGACCGGGCCGTATCGACGTTCATATCCATTTTCCACTCTGCGATTTCTCTGCTTTCAAGAATCTCGCAATCAATTATCTAGGCGTCAAGGATCACAAATTGTTCCCTCAGGTTGAAGAAATATTCCAAACCGGCGCCAGTTTGAGCCCCGCCGAGATCGGCGAACTGATGATCGCGAACCGAAACTCGCCTAGCCGAGCGATTAAATCGGTCATCTCAGCGTTGCAAACCGACGGCGACCGGCGGAGAGTCGGCAACGTCGTCCGGCGGCTGAGCGACTGCGGGTCGAGGAAGTCCGTCGCCGAATCCGTCGAATCGGGCGGCGTATTATGCAGCGAAAACACTCAGACCGGAAAAGAGTTCAGAAAATTGTACGGGTTTCTAAGGATGAAAAGTAACAAAATATCTCAGTCGTTCGATTCGTCTCCTATGCACAAAGAGAGTTGA

Protein sequence

MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKSIQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNVVRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKES
Homology
BLAST of Lsi05G021540 vs. ExPASy Swiss-Prot
Match: F4IJ77 (AAA-ATPase At2g46620 OS=Arabidopsis thaliana OX=3702 GN=At2g46620 PE=2 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 4.4e-171
Identity = 321/486 (66.05%), Postives = 383/486 (78.81%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           M   + SF ++ +     +L+RILLF+TGLI++V  W R + D FHVYQ +++PEFN+  
Sbjct: 1   MGILWDSFLLLLVSTFALFLVRILLFKTGLIYMVKLWRRKIIDWFHVYQFYKVPEFNDNV 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTN- 120
           Q+NHLY+KV  YL SLSS+E+SDFTNL TG K N+IILRLD NQ V D FLGA+V W N 
Sbjct: 61  QENHLYQKVYMYLNSLSSIENSDFTNLFTGKKSNEIILRLDRNQVVGDEFLGARVCWING 120

Query: 121 -EQNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMN------SKPNN 180
            +++ +RNFVLKIRKADKRRIL  YLQHIHT+ +DE+EQR  +LKLF+N           
Sbjct: 121 EDEDGARNFVLKIRKADKRRILGSYLQHIHTV-SDELEQRNTELKLFINVGIDDHLNKKK 180

Query: 181 QSDTRWKSIQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGP 240
           + + RW+SI F HP TFD+IAMETDLK KVKSDLESFLK KQYY+RLGRVWKRSYLLYGP
Sbjct: 181 KKNGRWRSIPFDHPCTFDNIAMETDLKNKVKSDLESFLKGKQYYNRLGRVWKRSYLLYGP 240

Query: 241 SGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKS 300
           SGTGKSSFVAAMANFL YDVYDIDL KV DDSDLK LLLQT GKS+IV+EDLDR L+ KS
Sbjct: 241 SGTGKSSFVAAMANFLDYDVYDIDLSKVVDDSDLKMLLLQTRGKSVIVIEDLDRHLSTKS 300

Query: 301 SALSLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAF 360
           +A++LS +LNF D IL+SC A+ER+MVFT+  KEQ++PA+LRPGR+DVHIHFPLCDF+AF
Sbjct: 301 TAVNLSGILNFTDSILSSCTADERIMVFTMTGKEQIDPAMLRPGRVDVHIHFPLCDFTAF 360

Query: 361 KNLAINYLGVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDG 420
           K LA NYLGVK+HKLF QVE IFQ GASLSPAEIGELMIANRNSP+RA+K VI+ALQTDG
Sbjct: 361 KTLANNYLGVKEHKLFSQVEGIFQNGASLSPAEIGELMIANRNSPTRALKHVINALQTDG 420

Query: 421 DRRRVGNVVRRLSDCGSRKSVAESV--ESGGVLC---SENTQTGKEFRKLYGFLRMKSNK 474
           DRR  G   R L + GSRKS +E V  +  G LC     ++   KEFRKLYG LR+KS++
Sbjct: 421 DRRGTGR--RLLLENGSRKSTSEDVSDDMSGSLCGGGGGSSPAVKEFRKLYGLLRIKSSR 480

BLAST of Lsi05G021540 vs. ExPASy Swiss-Prot
Match: Q9FLD5 (AAA-ATPase ASD, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AATP1 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.0e-66
Identity = 159/449 (35.41%), Postives = 248/449 (55.23%), Query Frame = 0

Query: 45  FHVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLEDSDFT-NLITGNKPNDIILRLDSN 104
           FH Y         E  +++ +Y  + +YL+  SS      T N I GNK   IIL +D +
Sbjct: 56  FHEYS-------GERFKRSDVYDAIQSYLSKDSSSRAKKLTANTIKGNK--SIILSMDDH 115

Query: 105 QTVQDNFLGAKVFWTNEQ--------------NSSRNFVLKIRKADKRRILRPYLQHIHT 164
           + + D F G KV+W +++              + SR ++LK  + D+  I + YL H+ +
Sbjct: 116 EEITDEFQGVKVWWQSKKHQSESRAISFYPKADESRFYMLKFHRRDREVITKKYLNHVIS 175

Query: 165 LTADEIEQRKGDLKLFMNSKPNNQS---DTRWKSIQFKHPSTFDSIAMETDLKEKVKSDL 224
                IE +  + KL+ N+   N S    T+W  + F+HP+TFD++AME   KE++K+DL
Sbjct: 176 -EGKTIEVKNRERKLYSNNPSQNWSGYKQTKWSHVTFEHPATFDTLAMEYKKKEEIKNDL 235

Query: 225 ESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDL 284
             F  SK YY ++G+ WKR YLL+GP GTGKS+ +AAMAN L YDVYD++L  V D+++L
Sbjct: 236 IKFSNSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANLLEYDVYDLELTTVKDNTEL 295

Query: 285 KFLLLQTTGKSIIVVEDLDRFL-------------------------------TEKSSAL 344
           + LL++T+GKSIIV+ED+D  L                                 K S +
Sbjct: 296 RRLLIETSGKSIIVIEDIDCSLDLTGQRKQKKDEEEDEDETSPIEKQMKKDQGENKGSKV 355

Query: 345 SLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNL 404
           +LS LLNF+DG L S C  ER++VFT N  ++++PA++R GR+D HI    C F AFK L
Sbjct: 356 TLSGLLNFIDG-LWSACGGERIIVFTTNFIDKLDPALIRKGRMDKHIEMSYCGFEAFKVL 415

Query: 405 AINYLGVK---DHKLFPQVEEIFQT-GASLSPAEIGELMIANRNSPSRAI--KSVISALQ 439
           A NYL  K   D++LF +++ + +     ++PA++GE ++      ++ I  K +I AL+
Sbjct: 416 ANNYLDAKEEDDNELFDEIKRLLEVEEIKMTPADVGENLLKKSEVETKEICLKRLIEALK 475

BLAST of Lsi05G021540 vs. ExPASy Swiss-Prot
Match: Q9LJJ7 (AAA-ATPase At3g28580 OS=Arabidopsis thaliana OX=3702 GN=At3g28580 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.5e-65
Identity = 146/424 (34.43%), Postives = 241/424 (56.84%), Query Frame = 0

Query: 45  FHVYQSFRIPEFN-ETSQQNHLYRKVSAYLTSLSSLEDSDF-TNLITGNKPNDIILRLDS 104
           F+ Y      E++ E  +++  Y  + +YL+  SS        N   G+K   I+L +D 
Sbjct: 47  FYPYIQITFHEYSGEHFKRSEAYLGIQSYLSKDSSARAKKLKANTTKGSK--SIVLSMDD 106

Query: 105 NQTVQDNFLGAKVFWTN--------------EQNSSRNFVLKIRKADKRRILRPYLQHIH 164
            + + D+F G +V+W +              E N  R ++L+  + D+  I+  YL+H+ 
Sbjct: 107 KEEITDDFEGIRVWWQSKKEGATRQSFSFYPEANEKRYYMLRFHRRDREVIIERYLEHV- 166

Query: 165 TLTADEIEQRKGDLKLFMNSK-PNNQSDTRWKSIQFKHPSTFDSIAMETDLKEKVKSDLE 224
                 IEQ+  + KL+ N+   ++ ++++W  + F+HP+TFD++AME + KE++KSDL 
Sbjct: 167 MREGKTIEQKNRERKLYSNTPGQSHGNNSKWSHVTFEHPATFDTLAMEENKKEEIKSDLI 226

Query: 225 SFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLK 284
            F KSK YY ++G+ WKR YLL+GP GTGKS+ +AAMANFL YDVYD++L  V D++ L+
Sbjct: 227 KFSKSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANFLEYDVYDLELTTVKDNTHLR 286

Query: 285 FLLLQTTGKSIIVVEDLDRFLT--------------------------------EKSSAL 344
            LL++T+ KSIIV+ED+D  L                                  K S +
Sbjct: 287 RLLIETSAKSIIVIEDIDCSLNLTGQRKKKEEEEEDGDDKNTIEKKMMMKNEGENKESKV 346

Query: 345 SLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNL 404
           +LS LLNF+DG L S C  ER++VFT N  ++++PA++R GR+D HI    C F AFK L
Sbjct: 347 TLSGLLNFIDG-LWSACGGERIIVFTTNFVDKLDPALIRKGRMDKHIEMSYCCFEAFKVL 406

Query: 405 AINYLGVKDHKLFPQVEEIFQT-GASLSPAEIGELMI--ANRNSPSRAIKSVISALQTDG 417
           A NYL V++ ++F +++ + +     ++PA++GE ++  + +      +K +I AL+ + 
Sbjct: 407 AKNYLDVEESEMFEEIKRLLEVEEIKMTPADVGENLLPKSEKEGGETCLKRLIEALKEEK 466

BLAST of Lsi05G021540 vs. ExPASy Swiss-Prot
Match: F4JPK8 (AAA-ATPase At4g30250 OS=Arabidopsis thaliana OX=3702 GN=At4g30250 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-64
Identity = 157/448 (35.04%), Postives = 240/448 (53.57%), Query Frame = 0

Query: 46  HVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLED-----SDFTNLITGNKPN--DIIL 105
           H+Y  F I E +  +  N LY  V  YL+S  ++ D     ++ T L     PN   +  
Sbjct: 48  HIY--FDITEIDGVN-TNELYNAVQLYLSSSVTVNDAVSSSNNNTRLSLTRVPNSSSVTF 107

Query: 106 RLDSNQTVQDNFLGAKVFWTN--------------EQNSSRNFVLKIRKADKRRILRPYL 165
            L +N  + D F G  + W +                   R F L+I K DK  +L  YL
Sbjct: 108 GLSNNDRITDVFNGVTILWEHVVVQRQVQSFSWRPMPEEKRGFTLQINKRDKALVLDSYL 167

Query: 166 QHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTR---WKSIQFKHPSTFDSIAMETDLKEK 225
            +I    ++EI +R  +  L+ NS+     D R   W S++FKHPSTFD++AM+ + K++
Sbjct: 168 DYI-VGKSEEIRRRNEERLLYTNSR-GVSLDARSHPWDSVRFKHPSTFDTLAMDPEKKKR 227

Query: 226 VKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVS 285
           +  DL  F   + +Y + GR WKR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V 
Sbjct: 228 IMEDLREFANGQGFYQKTGRAWKRGYLLYGPPGTGKSSLIAAMANYLGYDIYDLELTEVQ 287

Query: 286 DDSDLKFLLLQTTGKSIIVVEDLD---------------------------RFLTEKSSA 345
           ++S+L+ LL++T+ KSIIV+ED+D                             L E  S+
Sbjct: 288 NNSELRKLLMKTSSKSIIVIEDIDCSISLTKRGKNKKKNGSYEYDPGLTNGSGLEEPGSS 347

Query: 346 LSLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKN 405
           ++LS LLNF DG L SCC  E++ VFT N  E+++ A++R GR+D+H+H   C F A K 
Sbjct: 348 VTLSGLLNFTDG-LWSCCGSEKIFVFTTNHIEKLDSALMRSGRMDMHVHMGFCKFPALKI 407

Query: 406 LAINYLGVK----DHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQT 439
           L  NYL ++    D  +  ++EE  +  A ++PA++ E++I NR+   +A++ ++S L+ 
Sbjct: 408 LLKNYLRLEEEDMDSVVLKEMEECVEE-AEITPADVSEVLIRNRSDAEKAVREIVSVLKE 467

BLAST of Lsi05G021540 vs. ExPASy Swiss-Prot
Match: Q8RY66 (AAA-ATPase At4g25835 OS=Arabidopsis thaliana OX=3702 GN=At4g25835 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.1e-64
Identity = 163/463 (35.21%), Postives = 248/463 (53.56%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           M+ ++ S + +   + F   L   +F   L F ++K +      F  +  F I E +  +
Sbjct: 1   MKEYWTSLASLLGVLAFCQSLMNSVFPPELRFAISKLFNKFFKLFSTFCYFDITEIDGVN 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFW--- 120
             N LY  V  YL+S  S+  +   +L      + +   L +N ++ D F    V W   
Sbjct: 61  -TNELYNAVQLYLSSSVSIAGNRL-SLTRAVNSSSVTFGLSNNDSIVDTFNSVTVVWEHI 120

Query: 121 -TNEQNSS----------RNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFM 180
            T  Q  +          R F L+I+K DK  IL  YL +I    A+EI +   D  L+ 
Sbjct: 121 VTQRQTQTFAWRPMPEEKRGFTLRIKKKDKNLILDSYLDYIME-KANEIRRLNQDRLLYT 180

Query: 181 NSKPNNQSDTR---WKSIQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVW 240
           NS+     D+R   W+S+ FKHPSTFD++AM+   K+++  DL+ F + + +Y R GR W
Sbjct: 181 NSR-GGSLDSRGLPWESVPFKHPSTFDTLAMDPVKKQQIMEDLKDFAECQSFYERTGRAW 240

Query: 241 KRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVED 300
           KR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V  +S+L+ LL++T+ KSIIV+ED
Sbjct: 241 KRGYLLYGPPGTGKSSMIAAMANYLRYDIYDLELTEVKSNSELRKLLMKTSSKSIIVIED 300

Query: 301 LD--------------------RFLTEK--------SSALSLSALLNFMDGILTSCCAEE 360
           +D                      LT           + ++LS LLNF DG L SCC  E
Sbjct: 301 IDCSINLTNRNKKQSTGSYNEPEMLTGSGLGDDLGDGNTITLSGLLNFTDG-LWSCCGSE 360

Query: 361 RVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKL----FPQV 415
           R+ VFT N  E+++PA+LR GR+D+HIH   C FS+ K L  NYLG ++  L      ++
Sbjct: 361 RIFVFTTNHIEKLDPALLRSGRMDMHIHMSYCTFSSVKILLRNYLGFEEGDLNDVVLKEL 420

BLAST of Lsi05G021540 vs. ExPASy TrEMBL
Match: A0A1S3AVK0 (AAA-ATPase At2g46620 OS=Cucumis melo OX=3656 GN=LOC103483161 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.5e-259
Identity = 458/481 (95.22%), Postives = 466/481 (96.88%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFR F VIFIGICFSWLLRILLFRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETS
Sbjct: 1   MELFFRFFYVIFIGICFSWLLRILLFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           Q NHLYRKVSAYLTSLSSLEDSD+TNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDYTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           QN SRNFVLKIRKADKRRILRPYLQHIHTLTAD+ EQRKGDLKLFMNSKPNN SDTRWKS
Sbjct: 121 QNGSRNFVLKIRKADKRRILRPYLQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTT KS+IVVEDLDRFL EKSSALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANRNSPSRAIKSVISALQTDGDRRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAES+ESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSP+ K+
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKD 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. ExPASy TrEMBL
Match: A0A0A0L5T8 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119330 PE=3 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.4e-259
Identity = 458/481 (95.22%), Postives = 465/481 (96.67%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFR FS IFIGICFSWLLRILLFRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETS
Sbjct: 1   MELFFRFFSAIFIGICFSWLLRILLFRTGLIFLVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           Q NHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           Q  SRNFVL+IRKADKRRILRPYLQHIHTLTADE EQRKGDLKLFMNSKPNN SDTRWKS
Sbjct: 121 QKGSRNFVLRIRKADKRRILRPYLQHIHTLTADENEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTT KS+IVVEDLDRFL EKSSALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANRNSPSRAIKSVISALQTDGDRRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAES+ESGGV+CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSP+ KE
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVVCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKE 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. ExPASy TrEMBL
Match: A0A5A7U3P9 (AAA-ATPase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00800 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.6e-248
Identity = 437/458 (95.41%), Postives = 445/458 (97.16%), Query Frame = 0

Query: 24  LLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLEDSD 83
           L FRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETSQ NHLYRKVSAYLTSLSSLEDSD
Sbjct: 3   LFFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETSQHNHLYRKVSAYLTSLSSLEDSD 62

Query: 84  FTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQNSSRNFVLKIRKADKRRILRPY 143
           +TNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQN SRNFVLKIRKADKRRILRPY
Sbjct: 63  YTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQNGSRNFVLKIRKADKRRILRPY 122

Query: 144 LQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKSIQFKHPSTFDSIAMETDLKEKVK 203
           LQHIHTLTAD+ EQRKGDLKLFMNSKPNN SDTRWKSIQFKHPSTFDSIAMETDLKEKVK
Sbjct: 123 LQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKSIQFKHPSTFDSIAMETDLKEKVK 182

Query: 204 SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD 263
           SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD
Sbjct: 183 SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD 242

Query: 264 SDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSALLNFMDGILTSCCAEERVMVFTVN 323
           SDLKFLLLQTT KS+IVVEDLDRFL EKSSALSLSALLNFMDGILTSCCAEERVMVFTVN
Sbjct: 243 SDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSALLNFMDGILTSCCAEERVMVFTVN 302

Query: 324 CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP 383
           CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP
Sbjct: 303 CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP 362

Query: 384 AEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNVVRRLSDCGSRKSVAESVESGGVL 443
           AEI ELMIANRNSPSRAIKSVISALQTDGDRRRV N+ RRLSDCGSRKSVAES+ESGGVL
Sbjct: 363 AEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNIGRRLSDCGSRKSVAESIESGGVL 422

Query: 444 CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKES 482
           CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSP+ K+S
Sbjct: 423 CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKDS 460

BLAST of Lsi05G021540 vs. ExPASy TrEMBL
Match: A0A6J1E8T1 (AAA-ATPase At2g46620-like OS=Cucurbita moschata OX=3662 GN=LOC111431901 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.6e-247
Identity = 435/481 (90.44%), Postives = 457/481 (95.01%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFRSF VIFIGICF WLLRI+LFRTG +FVV K WRNLEDCFHVYQSFRIPEFN++S
Sbjct: 1   MELFFRSFFVIFIGICFCWLLRIVLFRTGFVFVVKKRWRNLEDCFHVYQSFRIPEFNQSS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           QQNHLYRKVSAYLTSLSSLEDSDFTNLITGN PNDIILRLDSNQTVQDNFLGAK+ WTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNAPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           +N SRNFVL+IRKADKRRILRPYLQHIHTLTAD IEQRKGDLKLFMNSKPNNQSDTRWKS
Sbjct: 121 ENGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSI+METDLKE+VKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSDDSDLK LLLQTT KSIIVVEDLDRFL +KSS LSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSTLSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCK+ V+PA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NRNSPSRAIKSVI+ALQTD +RRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAESVESGGVLCSENTQTGKE RKLYGFLRMKSNKISQ+FD+SPM K+
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDTSPMRKD 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. ExPASy TrEMBL
Match: A0A6J1IWA6 (AAA-ATPase At2g46620 OS=Cucurbita maxima OX=3661 GN=LOC111479067 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.9e-246
Identity = 433/481 (90.02%), Postives = 456/481 (94.80%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFRSF  IFIGICF WLLRI+LFRTG +FVV KWWRNLEDCFHVYQ FRIPEFN++S
Sbjct: 1   MELFFRSFFAIFIGICFCWLLRIVLFRTGFVFVVKKWWRNLEDCFHVYQFFRIPEFNQSS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           QQNHLYRKVSAYLTSLSSLEDSDFTNLITGN PNDIILRLDSNQTVQDNFLGAK+ WTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNTPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           +  SRNFVL+IRKADKRRILRPYLQHIHTLTAD IEQRKGDLKLFMNSKPNNQSDTRWKS
Sbjct: 121 EYGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQF+HPSTFDSI+METDLKE+VKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFQHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSDDSDLK LLLQTT KSIIVVEDLDRFL +KSSALSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDG+LTSCCAEERVMVFTVNCK+ V+PA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGLLTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NRNSPSRAIKSVI+ALQTD +RRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAESVESGGVLCSENTQTGKE RKLYGFLRMKSNKISQ+FD+SPM KE
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDASPMRKE 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. NCBI nr
Match: XP_038903225.1 (AAA-ATPase At2g46620 [Benincasa hispida])

HSP 1 Score: 907.9 bits (2345), Expect = 3.7e-260
Identity = 461/481 (95.84%), Postives = 467/481 (97.09%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFR FSVIFIGICF WLLR LLFRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETS
Sbjct: 1   MELFFRLFSVIFIGICFCWLLRNLLFRTGLIFLVKKWWGNLEDCFHVYQSFRIPEFNETS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           QN SRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS
Sbjct: 121 QNGSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSIAMETDLKE+VKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSDDSDLK LLLQTT KSIIVVEDLDRFL EKSSALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKSLLLQTTSKSIIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQ GASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVANI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAESVESGGV C+ENTQTGKEF+KLYGFLRMKSNKISQSFDSSP+HKE
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVFCNENTQTGKEFKKLYGFLRMKSNKISQSFDSSPLHKE 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. NCBI nr
Match: XP_008437854.1 (PREDICTED: AAA-ATPase At2g46620 [Cucumis melo])

HSP 1 Score: 904.8 bits (2337), Expect = 3.1e-259
Identity = 458/481 (95.22%), Postives = 466/481 (96.88%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFR F VIFIGICFSWLLRILLFRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETS
Sbjct: 1   MELFFRFFYVIFIGICFSWLLRILLFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           Q NHLYRKVSAYLTSLSSLEDSD+TNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDYTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           QN SRNFVLKIRKADKRRILRPYLQHIHTLTAD+ EQRKGDLKLFMNSKPNN SDTRWKS
Sbjct: 121 QNGSRNFVLKIRKADKRRILRPYLQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTT KS+IVVEDLDRFL EKSSALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANRNSPSRAIKSVISALQTDGDRRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAES+ESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSP+ K+
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKD 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. NCBI nr
Match: XP_004133798.1 (AAA-ATPase At2g46620 [Cucumis sativus])

HSP 1 Score: 903.7 bits (2334), Expect = 7.0e-259
Identity = 458/481 (95.22%), Postives = 465/481 (96.67%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFR FS IFIGICFSWLLRILLFRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETS
Sbjct: 1   MELFFRFFSAIFIGICFSWLLRILLFRTGLIFLVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           Q NHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           Q  SRNFVL+IRKADKRRILRPYLQHIHTLTADE EQRKGDLKLFMNSKPNN SDTRWKS
Sbjct: 121 QKGSRNFVLRIRKADKRRILRPYLQHIHTLTADENEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTT KS+IVVEDLDRFL EKSSALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANRNSPSRAIKSVISALQTDGDRRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAES+ESGGV+CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSP+ KE
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVVCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKE 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. NCBI nr
Match: KAA0048866.1 (AAA-ATPase [Cucumis melo var. makuwa] >TYK20818.1 AAA-ATPase [Cucumis melo var. makuwa])

HSP 1 Score: 868.2 bits (2242), Expect = 3.2e-248
Identity = 437/458 (95.41%), Postives = 445/458 (97.16%), Query Frame = 0

Query: 24  LLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLEDSD 83
           L FRTGLIF+V KWW NLEDCFHVYQSFRIPEFNETSQ NHLYRKVSAYLTSLSSLEDSD
Sbjct: 3   LFFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETSQHNHLYRKVSAYLTSLSSLEDSD 62

Query: 84  FTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQNSSRNFVLKIRKADKRRILRPY 143
           +TNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQN SRNFVLKIRKADKRRILRPY
Sbjct: 63  YTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQNGSRNFVLKIRKADKRRILRPY 122

Query: 144 LQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKSIQFKHPSTFDSIAMETDLKEKVK 203
           LQHIHTLTAD+ EQRKGDLKLFMNSKPNN SDTRWKSIQFKHPSTFDSIAMETDLKEKVK
Sbjct: 123 LQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKSIQFKHPSTFDSIAMETDLKEKVK 182

Query: 204 SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD 263
           SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD
Sbjct: 183 SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD 242

Query: 264 SDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSALLNFMDGILTSCCAEERVMVFTVN 323
           SDLKFLLLQTT KS+IVVEDLDRFL EKSSALSLSALLNFMDGILTSCCAEERVMVFTVN
Sbjct: 243 SDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSALLNFMDGILTSCCAEERVMVFTVN 302

Query: 324 CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP 383
           CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP
Sbjct: 303 CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP 362

Query: 384 AEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNVVRRLSDCGSRKSVAESVESGGVL 443
           AEI ELMIANRNSPSRAIKSVISALQTDGDRRRV N+ RRLSDCGSRKSVAES+ESGGVL
Sbjct: 363 AEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNIGRRLSDCGSRKSVAESIESGGVL 422

Query: 444 CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKES 482
           CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSP+ K+S
Sbjct: 423 CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKDS 460

BLAST of Lsi05G021540 vs. NCBI nr
Match: XP_023527367.1 (AAA-ATPase At2g46620-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 435/481 (90.44%), Postives = 457/481 (95.01%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           ME FFR F VIFIGICF WLLRI+LFRTG +FVV KWWRNLEDCFHVYQSFRIPEFN++S
Sbjct: 1   MELFFRLFFVIFIGICFCWLLRIVLFRTGFVFVVKKWWRNLEDCFHVYQSFRIPEFNQSS 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120
           QQNHLYRKVSAYLTSLSSLEDSDFTNLITGN PNDIILRLDSNQTVQDNFLGAK+ WTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNAPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 QNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180
           +N SRNFVL+IRKADKRRILRPYLQHIHTLTAD IEQRKGDLKLFMNSKPNNQSDTRWKS
Sbjct: 121 ENGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240
           IQFKHPSTFDSI+METDLKE+VKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKSSALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSDDSDLK LLLQTT KSIIVVEDLDRFL +KSS LSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSTLSLSAL 300

Query: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEERVMVFTVNCK+ V+PA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVGNV 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NRNSPSRAIKSVI+ALQTD +RRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 VRRLSDCGSRKSVAESVESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPMHKE 480
            RRLSDCGSRKSVAESVESGGVLCSENTQTGKE RKLYGFLRMKSNKISQ+FD+SPM K+
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDTSPMRKD 480

Query: 481 S 482
           S
Sbjct: 481 S 481

BLAST of Lsi05G021540 vs. TAIR 10
Match: AT2G46620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 602.4 bits (1552), Expect = 3.1e-172
Identity = 321/486 (66.05%), Postives = 383/486 (78.81%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           M   + SF ++ +     +L+RILLF+TGLI++V  W R + D FHVYQ +++PEFN+  
Sbjct: 1   MGILWDSFLLLLVSTFALFLVRILLFKTGLIYMVKLWRRKIIDWFHVYQFYKVPEFNDNV 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTN- 120
           Q+NHLY+KV  YL SLSS+E+SDFTNL TG K N+IILRLD NQ V D FLGA+V W N 
Sbjct: 61  QENHLYQKVYMYLNSLSSIENSDFTNLFTGKKSNEIILRLDRNQVVGDEFLGARVCWING 120

Query: 121 -EQNSSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMN------SKPNN 180
            +++ +RNFVLKIRKADKRRIL  YLQHIHT+ +DE+EQR  +LKLF+N           
Sbjct: 121 EDEDGARNFVLKIRKADKRRILGSYLQHIHTV-SDELEQRNTELKLFINVGIDDHLNKKK 180

Query: 181 QSDTRWKSIQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGP 240
           + + RW+SI F HP TFD+IAMETDLK KVKSDLESFLK KQYY+RLGRVWKRSYLLYGP
Sbjct: 181 KKNGRWRSIPFDHPCTFDNIAMETDLKNKVKSDLESFLKGKQYYNRLGRVWKRSYLLYGP 240

Query: 241 SGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVEDLDRFLTEKS 300
           SGTGKSSFVAAMANFL YDVYDIDL KV DDSDLK LLLQT GKS+IV+EDLDR L+ KS
Sbjct: 241 SGTGKSSFVAAMANFLDYDVYDIDLSKVVDDSDLKMLLLQTRGKSVIVIEDLDRHLSTKS 300

Query: 301 SALSLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAF 360
           +A++LS +LNF D IL+SC A+ER+MVFT+  KEQ++PA+LRPGR+DVHIHFPLCDF+AF
Sbjct: 301 TAVNLSGILNFTDSILSSCTADERIMVFTMTGKEQIDPAMLRPGRVDVHIHFPLCDFTAF 360

Query: 361 KNLAINYLGVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQTDG 420
           K LA NYLGVK+HKLF QVE IFQ GASLSPAEIGELMIANRNSP+RA+K VI+ALQTDG
Sbjct: 361 KTLANNYLGVKEHKLFSQVEGIFQNGASLSPAEIGELMIANRNSPTRALKHVINALQTDG 420

Query: 421 DRRRVGNVVRRLSDCGSRKSVAESV--ESGGVLC---SENTQTGKEFRKLYGFLRMKSNK 474
           DRR  G   R L + GSRKS +E V  +  G LC     ++   KEFRKLYG LR+KS++
Sbjct: 421 DRRGTGR--RLLLENGSRKSTSEDVSDDMSGSLCGGGGGSSPAVKEFRKLYGLLRIKSSR 480

BLAST of Lsi05G021540 vs. TAIR 10
Match: AT5G40010.1 (AAA-ATPase 1 )

HSP 1 Score: 255.8 bits (652), Expect = 7.1e-68
Identity = 159/449 (35.41%), Postives = 248/449 (55.23%), Query Frame = 0

Query: 45  FHVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLEDSDFT-NLITGNKPNDIILRLDSN 104
           FH Y         E  +++ +Y  + +YL+  SS      T N I GNK   IIL +D +
Sbjct: 56  FHEYS-------GERFKRSDVYDAIQSYLSKDSSSRAKKLTANTIKGNK--SIILSMDDH 115

Query: 105 QTVQDNFLGAKVFWTNEQ--------------NSSRNFVLKIRKADKRRILRPYLQHIHT 164
           + + D F G KV+W +++              + SR ++LK  + D+  I + YL H+ +
Sbjct: 116 EEITDEFQGVKVWWQSKKHQSESRAISFYPKADESRFYMLKFHRRDREVITKKYLNHVIS 175

Query: 165 LTADEIEQRKGDLKLFMNSKPNNQS---DTRWKSIQFKHPSTFDSIAMETDLKEKVKSDL 224
                IE +  + KL+ N+   N S    T+W  + F+HP+TFD++AME   KE++K+DL
Sbjct: 176 -EGKTIEVKNRERKLYSNNPSQNWSGYKQTKWSHVTFEHPATFDTLAMEYKKKEEIKNDL 235

Query: 225 ESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDL 284
             F  SK YY ++G+ WKR YLL+GP GTGKS+ +AAMAN L YDVYD++L  V D+++L
Sbjct: 236 IKFSNSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANLLEYDVYDLELTTVKDNTEL 295

Query: 285 KFLLLQTTGKSIIVVEDLDRFL-------------------------------TEKSSAL 344
           + LL++T+GKSIIV+ED+D  L                                 K S +
Sbjct: 296 RRLLIETSGKSIIVIEDIDCSLDLTGQRKQKKDEEEDEDETSPIEKQMKKDQGENKGSKV 355

Query: 345 SLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNL 404
           +LS LLNF+DG L S C  ER++VFT N  ++++PA++R GR+D HI    C F AFK L
Sbjct: 356 TLSGLLNFIDG-LWSACGGERIIVFTTNFIDKLDPALIRKGRMDKHIEMSYCGFEAFKVL 415

Query: 405 AINYLGVK---DHKLFPQVEEIFQT-GASLSPAEIGELMIANRNSPSRAI--KSVISALQ 439
           A NYL  K   D++LF +++ + +     ++PA++GE ++      ++ I  K +I AL+
Sbjct: 416 ANNYLDAKEEDDNELFDEIKRLLEVEEIKMTPADVGENLLKKSEVETKEICLKRLIEALK 475

BLAST of Lsi05G021540 vs. TAIR 10
Match: AT3G28580.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-66
Identity = 146/424 (34.43%), Postives = 241/424 (56.84%), Query Frame = 0

Query: 45  FHVYQSFRIPEFN-ETSQQNHLYRKVSAYLTSLSSLEDSDF-TNLITGNKPNDIILRLDS 104
           F+ Y      E++ E  +++  Y  + +YL+  SS        N   G+K   I+L +D 
Sbjct: 47  FYPYIQITFHEYSGEHFKRSEAYLGIQSYLSKDSSARAKKLKANTTKGSK--SIVLSMDD 106

Query: 105 NQTVQDNFLGAKVFWTN--------------EQNSSRNFVLKIRKADKRRILRPYLQHIH 164
            + + D+F G +V+W +              E N  R ++L+  + D+  I+  YL+H+ 
Sbjct: 107 KEEITDDFEGIRVWWQSKKEGATRQSFSFYPEANEKRYYMLRFHRRDREVIIERYLEHV- 166

Query: 165 TLTADEIEQRKGDLKLFMNSK-PNNQSDTRWKSIQFKHPSTFDSIAMETDLKEKVKSDLE 224
                 IEQ+  + KL+ N+   ++ ++++W  + F+HP+TFD++AME + KE++KSDL 
Sbjct: 167 MREGKTIEQKNRERKLYSNTPGQSHGNNSKWSHVTFEHPATFDTLAMEENKKEEIKSDLI 226

Query: 225 SFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLK 284
            F KSK YY ++G+ WKR YLL+GP GTGKS+ +AAMANFL YDVYD++L  V D++ L+
Sbjct: 227 KFSKSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANFLEYDVYDLELTTVKDNTHLR 286

Query: 285 FLLLQTTGKSIIVVEDLDRFLT--------------------------------EKSSAL 344
            LL++T+ KSIIV+ED+D  L                                  K S +
Sbjct: 287 RLLIETSAKSIIVIEDIDCSLNLTGQRKKKEEEEEDGDDKNTIEKKMMMKNEGENKESKV 346

Query: 345 SLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNL 404
           +LS LLNF+DG L S C  ER++VFT N  ++++PA++R GR+D HI    C F AFK L
Sbjct: 347 TLSGLLNFIDG-LWSACGGERIIVFTTNFVDKLDPALIRKGRMDKHIEMSYCCFEAFKVL 406

Query: 405 AINYLGVKDHKLFPQVEEIFQT-GASLSPAEIGELMI--ANRNSPSRAIKSVISALQTDG 417
           A NYL V++ ++F +++ + +     ++PA++GE ++  + +      +K +I AL+ + 
Sbjct: 407 AKNYLDVEESEMFEEIKRLLEVEEIKMTPADVGENLLPKSEKEGGETCLKRLIEALKEEK 466

BLAST of Lsi05G021540 vs. TAIR 10
Match: AT4G30250.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.9e-65
Identity = 157/448 (35.04%), Postives = 240/448 (53.57%), Query Frame = 0

Query: 46  HVYQSFRIPEFNETSQQNHLYRKVSAYLTSLSSLED-----SDFTNLITGNKPN--DIIL 105
           H+Y  F I E +  +  N LY  V  YL+S  ++ D     ++ T L     PN   +  
Sbjct: 48  HIY--FDITEIDGVN-TNELYNAVQLYLSSSVTVNDAVSSSNNNTRLSLTRVPNSSSVTF 107

Query: 106 RLDSNQTVQDNFLGAKVFWTN--------------EQNSSRNFVLKIRKADKRRILRPYL 165
            L +N  + D F G  + W +                   R F L+I K DK  +L  YL
Sbjct: 108 GLSNNDRITDVFNGVTILWEHVVVQRQVQSFSWRPMPEEKRGFTLQINKRDKALVLDSYL 167

Query: 166 QHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTR---WKSIQFKHPSTFDSIAMETDLKEK 225
            +I    ++EI +R  +  L+ NS+     D R   W S++FKHPSTFD++AM+ + K++
Sbjct: 168 DYI-VGKSEEIRRRNEERLLYTNSR-GVSLDARSHPWDSVRFKHPSTFDTLAMDPEKKKR 227

Query: 226 VKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVS 285
           +  DL  F   + +Y + GR WKR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V 
Sbjct: 228 IMEDLREFANGQGFYQKTGRAWKRGYLLYGPPGTGKSSLIAAMANYLGYDIYDLELTEVQ 287

Query: 286 DDSDLKFLLLQTTGKSIIVVEDLD---------------------------RFLTEKSSA 345
           ++S+L+ LL++T+ KSIIV+ED+D                             L E  S+
Sbjct: 288 NNSELRKLLMKTSSKSIIVIEDIDCSISLTKRGKNKKKNGSYEYDPGLTNGSGLEEPGSS 347

Query: 346 LSLSALLNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKN 405
           ++LS LLNF DG L SCC  E++ VFT N  E+++ A++R GR+D+H+H   C F A K 
Sbjct: 348 VTLSGLLNFTDG-LWSCCGSEKIFVFTTNHIEKLDSALMRSGRMDMHVHMGFCKFPALKI 407

Query: 406 LAINYLGVK----DHKLFPQVEEIFQTGASLSPAEIGELMIANRNSPSRAIKSVISALQT 439
           L  NYL ++    D  +  ++EE  +  A ++PA++ E++I NR+   +A++ ++S L+ 
Sbjct: 408 LLKNYLRLEEEDMDSVVLKEMEECVEE-AEITPADVSEVLIRNRSDAEKAVREIVSVLKE 467

BLAST of Lsi05G021540 vs. TAIR 10
Match: AT4G25835.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 4.3e-65
Identity = 163/463 (35.21%), Postives = 248/463 (53.56%), Query Frame = 0

Query: 1   MEFFFRSFSVIFIGICFSWLLRILLFRTGLIFVVNKWWRNLEDCFHVYQSFRIPEFNETS 60
           M+ ++ S + +   + F   L   +F   L F ++K +      F  +  F I E +  +
Sbjct: 1   MKEYWTSLASLLGVLAFCQSLMNSVFPPELRFAISKLFNKFFKLFSTFCYFDITEIDGVN 60

Query: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFW--- 120
             N LY  V  YL+S  S+  +   +L      + +   L +N ++ D F    V W   
Sbjct: 61  -TNELYNAVQLYLSSSVSIAGNRL-SLTRAVNSSSVTFGLSNNDSIVDTFNSVTVVWEHI 120

Query: 121 -TNEQNSS----------RNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFM 180
            T  Q  +          R F L+I+K DK  IL  YL +I    A+EI +   D  L+ 
Sbjct: 121 VTQRQTQTFAWRPMPEEKRGFTLRIKKKDKNLILDSYLDYIME-KANEIRRLNQDRLLYT 180

Query: 181 NSKPNNQSDTR---WKSIQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVW 240
           NS+     D+R   W+S+ FKHPSTFD++AM+   K+++  DL+ F + + +Y R GR W
Sbjct: 181 NSR-GGSLDSRGLPWESVPFKHPSTFDTLAMDPVKKQQIMEDLKDFAECQSFYERTGRAW 240

Query: 241 KRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTGKSIIVVED 300
           KR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V  +S+L+ LL++T+ KSIIV+ED
Sbjct: 241 KRGYLLYGPPGTGKSSMIAAMANYLRYDIYDLELTEVKSNSELRKLLMKTSSKSIIVIED 300

Query: 301 LD--------------------RFLTEK--------SSALSLSALLNFMDGILTSCCAEE 360
           +D                      LT           + ++LS LLNF DG L SCC  E
Sbjct: 301 IDCSINLTNRNKKQSTGSYNEPEMLTGSGLGDDLGDGNTITLSGLLNFTDG-LWSCCGSE 360

Query: 361 RVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKL----FPQV 415
           R+ VFT N  E+++PA+LR GR+D+HIH   C FS+ K L  NYLG ++  L      ++
Sbjct: 361 RIFVFTTNHIEKLDPALLRSGRMDMHIHMSYCTFSSVKILLRNYLGFEEGDLNDVVLKEL 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IJ774.4e-17166.05AAA-ATPase At2g46620 OS=Arabidopsis thaliana OX=3702 GN=At2g46620 PE=2 SV=1[more]
Q9FLD51.0e-6635.41AAA-ATPase ASD, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AATP1 PE=1 SV=1[more]
Q9LJJ72.5e-6534.43AAA-ATPase At3g28580 OS=Arabidopsis thaliana OX=3702 GN=At3g28580 PE=2 SV=1[more]
F4JPK82.7e-6435.04AAA-ATPase At4g30250 OS=Arabidopsis thaliana OX=3702 GN=At4g30250 PE=3 SV=1[more]
Q8RY666.1e-6435.21AAA-ATPase At4g25835 OS=Arabidopsis thaliana OX=3702 GN=At4g25835 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AVK01.5e-25995.22AAA-ATPase At2g46620 OS=Cucumis melo OX=3656 GN=LOC103483161 PE=3 SV=1[more]
A0A0A0L5T83.4e-25995.22AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119330 PE=3 SV... [more]
A0A5A7U3P91.6e-24895.41AAA-ATPase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00800 PE=... [more]
A0A6J1E8T18.6e-24790.44AAA-ATPase At2g46620-like OS=Cucurbita moschata OX=3662 GN=LOC111431901 PE=3 SV=... [more]
A0A6J1IWA61.9e-24690.02AAA-ATPase At2g46620 OS=Cucurbita maxima OX=3661 GN=LOC111479067 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038903225.13.7e-26095.84AAA-ATPase At2g46620 [Benincasa hispida][more]
XP_008437854.13.1e-25995.22PREDICTED: AAA-ATPase At2g46620 [Cucumis melo][more]
XP_004133798.17.0e-25995.22AAA-ATPase At2g46620 [Cucumis sativus][more]
KAA0048866.13.2e-24895.41AAA-ATPase [Cucumis melo var. makuwa] >TYK20818.1 AAA-ATPase [Cucumis melo var. ... [more]
XP_023527367.12.1e-24790.44AAA-ATPase At2g46620-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G46620.13.1e-17266.05P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G40010.17.1e-6835.41AAA-ATPase 1 [more]
AT3G28580.11.8e-6634.43P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G30250.11.9e-6535.04P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G25835.14.3e-6535.21P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 223..348
e-value: 0.0012
score: 28.1
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 228..346
e-value: 1.2E-12
score: 48.4
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 33..119
e-value: 1.8E-11
score: 44.2
NoneNo IPR availableGENE3D6.10.280.40coord: 344..408
e-value: 2.1E-6
score: 29.5
NoneNo IPR availablePANTHERPTHR23070:SF176BNAC04G00800D PROTEINcoord: 8..476
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 8..476
NoneNo IPR availableCDDcd00009AAAcoord: 224..346
e-value: 7.70729E-9
score: 52.5335
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 176..343
e-value: 7.8E-34
score: 119.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 156..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G021540.1Lsi05G021540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity