Lsi05G021200 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G021200
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING/U-box superfamily protein
Locationchr05: 28058594 .. 28064257 (-)
RNA-Seq ExpressionLsi05G021200
SyntenyLsi05G021200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGAATGTCAACGGGACAACTTCTTGTAATTTCACCTTGGCTTTCTGAGGGCAGGAGGATGTATTATTTGGCCATCCTATCCTTTTATACATGATTATTTGTTTAAAAATATCTTTTACTCAGACAAAAAAGTCAATCGTTTTCTCAGTTGCTCCTGTTAATTGTTCTATGATGCAACAGGTACAGGCTGCTGTGGCTGAAAAAGACCAGCCTAAATGGTGGGAAAAGAATGCACAAAACATGATTGATATACATTCGACACAGGAATTTTTGACTGCATTAAGTGAAGCTGGTGATAGATTAGTTATTGTAGAATTTTATGGAACCTGGTGTGCTTCTTGCAGGGCATTATTTCCCCGGGTAACTCTCTTCATCAGTGCTTTATATTTCTCAGATTGTGTAGTTGGTCTGTTGCTTACATTTGTCGTTAGATAATCAGGACATGCTTTCAGCATTTTCTAAAACTCGATGTAGGACCAGCTTATCGAAGTAGGCATTAAATTAATTTGTGTCATCAGAGACAATGACTGCGTCATTTGTTGGCTATCAAGATGAAAACTTTTTTTTAAAATACACTTTGTATTTCTTAAGATTTTGTACCTATCTATGTTGATGAGGTTGAAACTACAGACGATTGCTCAATCCTTTAAAAATTTGTAATCAAGAAGGGAGAACTCTTGAATATTGTCCCTTTTTATAATTGAACTTGTTTTTCTTTCCAAAAGAATATTTGTCCCTTCGGGGGCATCAGATAAAATGAACTCTGGAATATTTCATATCCCAAGTAGCTTGTCTCAAGATACTCTGGATAGATGATCCTGTGATCTTATTTGCACGGTTGTTTCTTGCAAATGCTGCTCTCATCAAATGATTGGTATTCATTGAATTTGATATCTGTTGTCTTCTATAAAGAAAATGCATCTTACAGCAAAATTTGAGCTTGCTACTATCATAATTTTTACTTTTCAGTTTTATGTTCTGATGTTTTTATATATGTTGAAATGGAACTTTCAGCTCTGCAGAACAGCTGATGAACACCCAGAGATTTTATTCCTAAAAGTCAATTTTGATGAAAACAAGCCAATGTGCAAAAGTTTGAATGTCAAGGTCCTTCCTTATTTCCACTTTTATCGTGGTGCTGACGGGCAGTTGGAGTCCTTCTCATGCTCACTCGCAAAGGTTAGCCCCCCTCCCCATCCCCACCCCCACCCCCAATGTGTCAATTCATTCTATTTTATTGGTTAGCTTATAATGAGTAGAATGTATTTTTTGAGATATAAATAGAATTTTAAGTCAATTCGCTCTATTTCATTGGTTAGCTTATATTGAGCAGAATATATTTTAAAATCTGAATAACTTTTCGAGTCGTGGACACATGTGATAGAAGGATCGATGAAGATTTCCAATATGGATAGAAAACTTTCCTTATCCTCATATTTGAATAACTACATTAAGTTGTTCATCTGGCAGTGATTTTTTCTTAGTATTTTCATCAAATGATCTATATAGGTGAACATTTTCTAAATTTTAACTCTATCTAACCCTTCCCCATCCCACAGTTTCAGAAAATTAAAGATGCAATTGAGATGCACAACACAGCTCGTTGCAGCATTGGGCCGCCAAAGGGAGTCGGAGATCTTATACTGGAGCCCTCCTTGGCTCCTAAAGACAAACAATAAACAGAAGCTTCATCATAGATTTGCTGCCAAATCTGCGTCGGTATCATTGTGAATTCCCCTGATATTAATATAACCTTCTTGTTTAAAAAAGATTCTGCAGTCAGCTATCCAATCTGTAAATTTGCATTATTGTTCTGTAAAAGAATTCAGAAGTTGAGAATACCAATCATAAAATATTTTGCTGCCTTTATCTGCCTTCTTGTGGTAGCTTTGACGTCTGTAATTTGAAACTGGTTATATTGGATCAAGTTCTAAATAGATGCCATTGATTCATTTGGTTTGATGGGCAGGAGCTTACATATACTGAAAACTGATCCTGGGATTTGACAGTTCTCTACTGTACGTTTAAGTTAACGTTCTGCCAACTAGGTAAAACGTTTCTAATTTCCATTAAAAAAAAGAAAAAGAGTTCGAATCTCCATGTTCTCAGGGAGCTATTATTTAGAAGCTAAACTATGAAAGTATGAATATCCTGTCATGAGCATTCGTCATAATGAAAAAGAAACTCTTTTACTTTTGCGTGCCAAAGCAGCAGTAGCACTGTTTGTTCTGGCAACAGTCAATAGCTACTTTTTATATGCTTTGAATCATAAATTTAGTCACTAAATGATGGACATACTTATTCAATCTTTAAACTTCCAATTTGGGTTGTCTTATTCAATCTTTAAACTTCCAATTTGGGTTGTAATGATTCAATCGTCTGCGTTATATCAGGTTAGACTAACTAGTAAAGATAATAGATTACACAGAGAATAAGTTATCTAGATAAATTTAAACCAAGTGTTTAATTTTAAAAATAAGTTATTTTAGAAAAAAATAGAGTGTTTGACAACTACTCAAAATAACTTTTGAAACACTTTTAATTCAAAGTGCATCTTAAACAATTTTTATCAAAAGAGTATAAATAATAATAACTTTTTTTAAAAACATTTTTTTCTATAGTCAATCTAAATAGACCCTCCTTTAAGCTTTCATAATTTAAATTCCTTGCGATTCTGTCCCAAAAAGACAAAAAAAAAAAAAAAAAAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGAAAGTAATTGAAGGACACAATTGACAAATGAGGTTGCACACCACAGTACAAGTTGAAACATATCTAATTTTGACACAAACAGATATAGTTATATATAGAAACTTTGGAAGTTACTAATTCCTATATGTTTTTCCTTGAATTAAATAAGGACAAAATATAGAAAGAGAAACTTTGGAAGTTTATATCAAATTGAAATTCTTAATTGAACATCTTTTTAATAATGTTATGGTCATCTTCAATTCTGTAGCTTTCCTTCATCAGTATTAAAATTATAAGCTCATGTTACTTGTGACGTCCACTACTAAAGGTCAATCTACAAGCACCAAAACTTCTGACTTTAGAATAATTTGATCAAGTCATAATAACATTATGATTTATTCAAACCCAACGATGAATAAAATGTTCAAGTTTTAAAGCTTACAAAAAATTTCATTTAGCCAAGCTAATGGATTTCTCACACCCCTTTCTCTTCTTCTTCTTCCTCTTCATCTTCCCCACTTTATGCACAGCTCAAAGTTGCTCTATATCCAGCTGTATCAACGACAATTTCGACATCAGATTCCCCTTCCGAATTGCAGACCAGCAACCTGCAAGATGTGGCTATCCTGGTTTCAACTTGGTCTGCAACAAAGCGGGTGTGGCAACTATAAATCTATCTTCTTCTGAATATTTTTTGGTAAGAGGTATTGATTATGTCACTCAACAAATACAGCTCTATGATTCAGATGCTTGCCTTCCTAGAAGACTTCTCCAAGGCTTCAAACTCAATCTCTCAAATTCCCCATTTATACCACTTTCTAGTCAGAATTTTACCTTTCTAAGCTGCCCATCTCAGTTTGCAATGTCGCGTTTTCCTGTCATTGGTTGTCTTAGCAATTCAACCGACTCGGTTGTAGCTACTTCCTCGATGAGTTTTGCCAAGTCAATGTCAACCTCTTGCAAGATCATGGCTACTATATCTATCCCAGTTTCAAACCCAGACGAGGCCAATCAATTTTTAACTAACCTCAATGGTGAGCTCGTGCTAACTTGGTATAGCCCTGCTTGCGGTATCTGCGAGACAGAAGGCAGGCTTTGTGGCTACAAAAGCAATAGTGGTCAGGGGATTGCCTGTTATAGCAAGTACACATCAGGTAAAAATTCTGCCCTTTGTAACTATTTCCTAACTTTATGTTCATCTTTAAACTATATCTCACATAACAGCTCAAAAATTCATCTTTTACAATTGGTGGAATGCATTTCTTCAACATCTCTTGTATGCATCCTAGTTGGTTGAGGAAAAGCTCAAAACGAATACATAATGAATATTGAAAAAAGAAAAGAGAAAACAAAACCATTGTTTTCTTAAAATCATAATAAACAAACCAATGTCCCAATAATAATGGCGGTATTACCTATTACCCTAAACTGCAGGCTGCTATGTGAATCTTAGACTGGCTGTTGGTTGACCAAGACATAGATAATGTTTATATTACTGGGAAGTTGGGATTAAAGTAATACCTAAAGAATAATTAAAAAGAAGTTGACTTTATAGATGGTGAAGGAATGACAAAGACATTTGACATTTGTTCACTGCAGCACGTCTCTCAATAATCTTCAAACTGGATCAAATATGTCATATATAACCTTAAAAACTTTTCATCTTTCACAACTAGCATCCTTTTTAGCAATTCGTTGAAATATTTTTTCTCGGTCCTCCTCAGCCGAGATCGACCCCGAGATTGGAATAAAAATTTGACTTCGCACATTCAAAGGCTTTCTCAATAGAATCTTTTGACGATTGATTTTAATATTTATGGTAAGTGATTGTTAGTTTGGACGTACGATGAAAAGAAGTCAAATTTTTATTCGAATCTCGATCGGGAACGAAAAACTATTTCAATGAGTTCTCCAAAAAGGTGGTGCTAGTTGTGAAAGATGAAAACATTTGAAGGTTATCTTTGACAGTTTTCCCACATACACAGAAAAAATGTCATTCATGATATGTTTTCCAAAAATATCAAAGTTCATGAAGCCCCATTTAAATGATAGTCACCTTAAATTTCAGAGGAAAACAATGGTCTCAGAGTATTGCGGATTATCTGTGTGGTGATTGTTCTACCGACTTTGATGTGTGTCTTTGGACTTGGCTGTTTTATCTGCTTCGCAAAGTGGGGCTATGGCCTTGCGGACGGCAGAGGAAACCAAGTTCAGCATCAGCAGCAGGTCAATCCAGCAGGGTCTGATTTAGAGGCACCGACCCGTTTGAGTGGTCTTGACGAATCCACCATTGAATCATATCAAAAGGTAATCCTTGGAGAAAGCCGGCGGCTGCCTGGCCCCAATGGCACCACCTGCCCCATATGCCTTGGAGAGTACTTAACCAAAGACACTGTCAGATGCATTCCTGAGTGCAAGCATTGCTTCCATGTTGATTGTATTGATCAATGGCTTCGAGTCAACAGCAGCTGCCCTGTTTGTCGAAACTCTCCCTCTCCCTCCCCTTCCCATTTAACTTCAATCTCATGA

mRNA sequence

TTTGGAATGTCAACGGGACAACTTCTTGTAATTTCACCTTGGCTTTCTGAGGGCAGGAGGATGTATTATTTGGCCATCCTATCCTTTTATACATGATTATTTGTTTAAAAATATCTTTTACTCAGACAAAAAAGTCAATCGTTTTCTCAGTTGCTCCTGTTAATTGTTCTATGATGCAACAGGTACAGGCTGCTGTGGCTGAAAAAGACCAGCCTAAATGGTGGGAAAAGAATGCACAAAACATGATTGATATACATTCGACACAGGAATTTTTGACTGCATTAAGTGAAGCTGGTGATAGATTAGTTATTGTAGAATTTTATGGAACCTGGTGTGCTTCTTGCAGGGCATTATTTCCCCGGCTCTGCAGAACAGCTGATGAACACCCAGAGATTTTATTCCTAAAAGTCAATTTTGATGAAAACAAGCCAATGTGCAAAAGTTTGAATGTCAAGGTCCTTCCTTATTTCCACTTTTATCGTGGTGCTGACGGGCAGTTGGAGTCCTTCTCATGCTCACTCGCAAAGGTTAGCCCCCCTCCCCATCCCCACCCCCACCCCCAATGTGTCAATTCATTCTATTTTATTGAAGGATCGATGAAGATTTCCAATATGGATAGAAAACTTTCCTTATCCTCATATTTGAATAACTACATTAAGTTGTTCATCTGGCATTTCAGAAAATTAAAGATGCAATTGAGATGCACAACACAGCTCGTTGCAGCATTGGGCCGCCAAAGGGAGTCGGAGATCTTATACTGGAGCCCTCCTTGGCTCCTAAAGACAAACAATAAACAGAAGCTTCATCATAGATTTGCTGCCAAATCTGCGTCGCTCAAAGTTGCTCTATATCCAGCTGTATCAACGACAATTTCGACATCAGATTCCCCTTCCGAATTGCAGACCAGCAACCTGCAAGATGTGGCTATCCTGGTTTCAACTTGGTCTGCAACAAAGCGGCTCTATGATTCAGATGCTTGCCTTCCTAGAAGACTTCTCCAAGGCTTCAAACTCAATCTCTCAAATTCCCCATTTATACCACTTTCTAGTCAGAATTTTACCTTTCTAAGCTGCCCATCTCAGTTTGCAATGTCGCGTTTTCCTGTCATTGGTTGTCTTAGCAATTCAACCGACTCGGTTGTAGCTACTTCCTCGATGAGTTTTGCCAAGTCAATGTCAACCTCTTGCAAGATCATGGCTACTATATCTATCCCAGTTTCAAACCCAGACGAGGCCAATCAATTTTTAACTAACCTCAATGGTGAGCTCGTGCTAACTTGGTATAGCCCTGCTTGCGGTATCTGCGAGACAGAAGGCAGGCTTTGTGGCTACAAAAGCAATAGTGGTCAGGGGATTGCCTGTTATAGCAAGTACACATCAGAGGAAAACAATGGTCTCAGAGTATTGCGGATTATCTGTGTGGTGATTGTTCTACCGACTTTGATGTGTGTCTTTGGACTTGGCTGTTTTATCTGCTTCGCAAAGTGGGGCTATGGCCTTGCGGACGGCAGAGGAAACCAAGTTCAGCATCAGCAGCAGGTCAATCCAGCAGGGTCTGATTTAGAGGCACCGACCCGTTTGAGTGGTCTTGACGAATCCACCATTGAATCATATCAAAAGGTAATCCTTGGAGAAAGCCGGCGGCTGCCTGGCCCCAATGGCACCACCTGCCCCATATGCCTTGGAGAGTACTTAACCAAAGACACTGTCAGATGCATTCCTGAGTGCAAGCATTGCTTCCATGTTGATTGTATTGATCAATGGCTTCGAGTCAACAGCAGCTGCCCTGTTTGTCGAAACTCTCCCTCTCCCTCCCCTTCCCATTTAACTTCAATCTCATGA

Coding sequence (CDS)

ATGATTATTTGTTTAAAAATATCTTTTACTCAGACAAAAAAGTCAATCGTTTTCTCAGTTGCTCCTGTTAATTGTTCTATGATGCAACAGGTACAGGCTGCTGTGGCTGAAAAAGACCAGCCTAAATGGTGGGAAAAGAATGCACAAAACATGATTGATATACATTCGACACAGGAATTTTTGACTGCATTAAGTGAAGCTGGTGATAGATTAGTTATTGTAGAATTTTATGGAACCTGGTGTGCTTCTTGCAGGGCATTATTTCCCCGGCTCTGCAGAACAGCTGATGAACACCCAGAGATTTTATTCCTAAAAGTCAATTTTGATGAAAACAAGCCAATGTGCAAAAGTTTGAATGTCAAGGTCCTTCCTTATTTCCACTTTTATCGTGGTGCTGACGGGCAGTTGGAGTCCTTCTCATGCTCACTCGCAAAGGTTAGCCCCCCTCCCCATCCCCACCCCCACCCCCAATGTGTCAATTCATTCTATTTTATTGAAGGATCGATGAAGATTTCCAATATGGATAGAAAACTTTCCTTATCCTCATATTTGAATAACTACATTAAGTTGTTCATCTGGCATTTCAGAAAATTAAAGATGCAATTGAGATGCACAACACAGCTCGTTGCAGCATTGGGCCGCCAAAGGGAGTCGGAGATCTTATACTGGAGCCCTCCTTGGCTCCTAAAGACAAACAATAAACAGAAGCTTCATCATAGATTTGCTGCCAAATCTGCGTCGCTCAAAGTTGCTCTATATCCAGCTGTATCAACGACAATTTCGACATCAGATTCCCCTTCCGAATTGCAGACCAGCAACCTGCAAGATGTGGCTATCCTGGTTTCAACTTGGTCTGCAACAAAGCGGCTCTATGATTCAGATGCTTGCCTTCCTAGAAGACTTCTCCAAGGCTTCAAACTCAATCTCTCAAATTCCCCATTTATACCACTTTCTAGTCAGAATTTTACCTTTCTAAGCTGCCCATCTCAGTTTGCAATGTCGCGTTTTCCTGTCATTGGTTGTCTTAGCAATTCAACCGACTCGGTTGTAGCTACTTCCTCGATGAGTTTTGCCAAGTCAATGTCAACCTCTTGCAAGATCATGGCTACTATATCTATCCCAGTTTCAAACCCAGACGAGGCCAATCAATTTTTAACTAACCTCAATGGTGAGCTCGTGCTAACTTGGTATAGCCCTGCTTGCGGTATCTGCGAGACAGAAGGCAGGCTTTGTGGCTACAAAAGCAATAGTGGTCAGGGGATTGCCTGTTATAGCAAGTACACATCAGAGGAAAACAATGGTCTCAGAGTATTGCGGATTATCTGTGTGGTGATTGTTCTACCGACTTTGATGTGTGTCTTTGGACTTGGCTGTTTTATCTGCTTCGCAAAGTGGGGCTATGGCCTTGCGGACGGCAGAGGAAACCAAGTTCAGCATCAGCAGCAGGTCAATCCAGCAGGGTCTGATTTAGAGGCACCGACCCGTTTGAGTGGTCTTGACGAATCCACCATTGAATCATATCAAAAGGTAATCCTTGGAGAAAGCCGGCGGCTGCCTGGCCCCAATGGCACCACCTGCCCCATATGCCTTGGAGAGTACTTAACCAAAGACACTGTCAGATGCATTCCTGAGTGCAAGCATTGCTTCCATGTTGATTGTATTGATCAATGGCTTCGAGTCAACAGCAGCTGCCCTGTTTGTCGAAACTCTCCCTCTCCCTCCCCTTCCCATTTAACTTCAATCTCATGA

Protein sequence

MIICLKISFTQTKKSIVFSVAPVNCSMMQQVQAAVAEKDQPKWWEKNAQNMIDIHSTQEFLTALSEAGDRLVIVEFYGTWCASCRALFPRLCRTADEHPEILFLKVNFDENKPMCKSLNVKVLPYFHFYRGADGQLESFSCSLAKVSPPPHPHPHPQCVNSFYFIEGSMKISNMDRKLSLSSYLNNYIKLFIWHFRKLKMQLRCTTQLVAALGRQRESEILYWSPPWLLKTNNKQKLHHRFAAKSASLKVALYPAVSTTISTSDSPSELQTSNLQDVAILVSTWSATKRLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTDSVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETEGRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGYGLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS
Homology
BLAST of Lsi05G021200 vs. ExPASy Swiss-Prot
Match: P0CH01 (Putative RING-H2 finger protein ATL21A OS=Arabidopsis thaliana OX=3702 GN=ATL21A PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.0e-57
Identity = 129/291 (44.33%), Postives = 177/291 (60.82%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFA-MSRFPVIGCLSNSTD 348
           RLYD + CL R+LL     ++S SPF  L   ++TFLSCP++ A  SRF  I CL NST 
Sbjct: 90  RLYDPENCLARKLL---TFDISRSPFSALYLVSYTFLSCPNEVAKSSRFDSIPCLGNSTT 149

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNP--DEANQFLTNLNG-ELVLTWYSPACGIC 408
           S +AT+S+  AKSM  SC+I+ T+ +PVS     + ++F T++N  +L L W SP+C  C
Sbjct: 150 SFLATTSLDLAKSMLPSCQIVKTLDVPVSRRVIAKKSRFSTDVNDKDLWLKWDSPSCSDC 209

Query: 409 ETEGRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAK 468
           E +   CG++SN+   + C+    S  N   +VL+II + I+ P  +    +   +C ++
Sbjct: 210 ERDFLRCGFRSNTSLQVKCFPFENSGYNTEPQVLKIILLSIIGPLTIFATCIAVGVCTSE 269

Query: 469 WGYGLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPN-G 528
               L          Q+ V  A          +GLDES IESY+K  LGESRRLPG N  
Sbjct: 270 RFASLI---------QRNVAIAALQPNEVIVTTGLDESIIESYKKTELGESRRLPGNNDD 329

Query: 529 TTCPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPS 575
             CPICL EY +K+TVRCIPEC HCFH +CID WL+++ SCP+CRNSPSP+
Sbjct: 330 IVCPICLSEYASKETVRCIPECDHCFHSECIDVWLKIHGSCPLCRNSPSPA 368

BLAST of Lsi05G021200 vs. ExPASy Swiss-Prot
Match: Q8LCT3 (Thioredoxin-like 2-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g29670 PE=2 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 2.8e-55
Identity = 99/128 (77.34%), Postives = 112/128 (87.50%), Query Frame = 0

Query: 30  QVQAAVAEKDQPKWWEKNAQNMIDIHSTQEFLTALSEAGDRLVIVEFYGTWCASCRALFP 89
           +V   VAE + PKWWE+NA NM+DIHST+EFL+ALS AG+RLVIVEFYGTWCASCRALFP
Sbjct: 84  KVDENVAETEPPKWWERNAPNMVDIHSTEEFLSALSGAGERLVIVEFYGTWCASCRALFP 143

Query: 90  RLCRTADEHPEILFLKVNFDENKPMCKSLNVKVLPYFHFYRGADGQLESFSCSLAKVSPP 149
           +LC+TA EHP+I+FLKVNFDENKPMCKSLNV+VLP+FHFYRGADGQLESFSCSLAKV   
Sbjct: 144 KLCKTAVEHPDIVFLKVNFDENKPMCKSLNVRVLPFFHFYRGADGQLESFSCSLAKVKKA 203

Query: 150 PHPHPHPQ 158
               P PQ
Sbjct: 204 ISVSPFPQ 211

BLAST of Lsi05G021200 vs. ExPASy Swiss-Prot
Match: Q5TKD8 (Thioredoxin-like 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0200100 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.0e-53
Identity = 100/118 (84.75%), Postives = 108/118 (91.53%), Query Frame = 0

Query: 30  QVQAAVAEK-DQPKWWEKNA-QNMIDIHSTQEFLTALSEAGDRLVIVEFYGTWCASCRAL 89
           +  AAV++K +QPKWWEKNA  NMIDIHSTQEFL AL +AGDRLVIVEFYGTWC SCRAL
Sbjct: 58  KAHAAVSDKSEQPKWWEKNAGPNMIDIHSTQEFLDALRDAGDRLVIVEFYGTWCGSCRAL 117

Query: 90  FPRLCRTADEHPEILFLKVNFDENKPMCKSLNVKVLPYFHFYRGADGQLESFSCSLAK 146
           FPRLCRTA E+P+ILFLKVNFDENKPMCK LNVKVLPYFHFYRGADGQLE+FSCSLAK
Sbjct: 118 FPRLCRTAVENPDILFLKVNFDENKPMCKRLNVKVLPYFHFYRGADGQLEAFSCSLAK 175

BLAST of Lsi05G021200 vs. ExPASy Swiss-Prot
Match: Q9SKK8 (RING-H2 finger protein ATL22 OS=Arabidopsis thaliana OX=3702 GN=ATL22 PE=2 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 1.9e-51
Identity = 127/300 (42.33%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAM-SRFPVIGCLSNSTD 348
           RL D + CL RRLL     + S SPF  L S+N+TFL CP +  + + F  I CL N+T 
Sbjct: 93  RLNDPENCLARRLL---NFDPSGSPFSFLRSRNYTFLICPKEANITASFRAIDCLGNTTS 152

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVS-------NPDEANQFLTNLNGELVLTWYSPA 408
           S       +   SM +SC I   + +P S        PD  N      + ++ L W SP 
Sbjct: 153 SFFVVQFENLG-SMPSSCHIFKILPLPFSWFVAYTTYPDGQN------SRDMWLKWDSPD 212

Query: 409 CGICE--TEGRLCGYKSNSGQGIACYSKYT-SEENNGLRVLRIICVVIVLPTLMCVFGLG 468
           C  CE  T  R CG+K+N+   + C+S       N GL+VL+I+C+ +V P     F +G
Sbjct: 213 CRDCERRTNSR-CGFKNNTSHQVECFSSVNPGLHNTGLQVLKIMCLSLVGPLTALTFCVG 272

Query: 469 CFICFAKWGYGLADGRGNQVQHQQQVNPAGSDLEAPTR---LSGLDESTIESYQKVILGE 528
             +C        ++   +Q+Q       +GS    P+      GLDESTIESY+KV LGE
Sbjct: 273 LVMC-------SSERVSSQIQQAVVARLSGSVTSQPSNEVARIGLDESTIESYKKVELGE 332

Query: 529 SRRLP-GPNGTTCPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSP 574
           SRRLP G N   CPICL EY TK+TVRC+PEC+HCFH +CID WL+++SSCPVCR++PSP
Sbjct: 333 SRRLPTGSNDVVCPICLSEYATKETVRCLPECEHCFHTECIDAWLKLHSSCPVCRSNPSP 374

BLAST of Lsi05G021200 vs. ExPASy Swiss-Prot
Match: P0CH02 (Putative RING-H2 finger protein ATL21B OS=Arabidopsis thaliana OX=3702 GN=ATL21B PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.5e-51
Identity = 114/283 (40.28%), Postives = 162/283 (57.24%), Query Frame = 0

Query: 292 DSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAM-SRFPVIGCLSNSTDSVV 351
           D + CL +RLL     N+S SPF P     +TFL+CP++  + S +P I CLSNST S  
Sbjct: 92  DPENCLAKRLL---TFNISGSPFSPRFDTLYTFLTCPNELVLPSWYPSIPCLSNSTSSFF 151

Query: 352 ATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETEGRL 411
           ATS+ + A+SM  SC+I+  I +P  +P    +F + LN  L+L W SP C  CE +   
Sbjct: 152 ATSNFALAESMLPSCQIVKRIYVPADSPFAETRFSSYLNQSLLLEWNSPNCRGCEIDYLR 211

Query: 412 CGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGYGLA 471
           CG+K+ +   + C+    S   +   V  +IC+ I+   ++ V       C A     + 
Sbjct: 212 CGFKNKASPEVKCFGAKKSGHLSRAVVAVLICLSIIGAVILFV------TCIA-----IR 271

Query: 472 DGRGNQVQHQQQVNPAGSDLEAPTRL---SGLDESTIESYQKVILGESRRLPGPNGTTCP 531
                + +H      A + ++ P  +    GLD+STIE Y+ + LGESRR PG NG  CP
Sbjct: 272 IHNTPRRRHWAVPAAAATVMQQPREVMATRGLDQSTIEKYKTMELGESRRPPGTNGIVCP 331

Query: 532 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNS 571
           ICL EY++K+TVR IPEC HCFH  CID WL+++ SCP+CRNS
Sbjct: 332 ICLSEYVSKETVRFIPECDHCFHAKCIDVWLKIHGSCPLCRNS 360

BLAST of Lsi05G021200 vs. ExPASy TrEMBL
Match: A0A5A7TZ53 (Putative RING-H2 finger protein ATL21A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G001060 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 1.2e-149
Identity = 256/295 (86.78%), Postives = 271/295 (91.86%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYDSD CLPRR LQG  KLN SNSPFIPL SQN TFLSCP QF MS FP+IGCLSNST+
Sbjct: 87  QLYDSDDCLPRRFLQGLNKLNFSNSPFIPLFSQNITFLSCPPQFTMSHFPIIGCLSNSTN 146

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           S++ATSS SF KSMSTSC IM T+ +PVSNPDE NQF TNLNG+LVLTWYSPACGICETE
Sbjct: 147 SILATSSTSFVKSMSTSCTIMTTLPVPVSNPDETNQFSTNLNGDLVLTWYSPACGICETE 206

Query: 409 GRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGY 468
           GRLCGYKSNSGQGIAC+ KYTSEENNGLRVLRIICVVI+LPTLMCV GLGCFIC AKW Y
Sbjct: 207 GRLCGYKSNSGQGIACFDKYTSEENNGLRVLRIICVVILLPTLMCVIGLGCFICMAKWSY 266

Query: 469 GLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 528
            L DGRGNQVQH+QQVNPAGSDLEAP+RLSGL+ESTIESYQKVILGESRRLPGPNGTTCP
Sbjct: 267 SLTDGRGNQVQHRQQVNPAGSDLEAPSRLSGLNESTIESYQKVILGESRRLPGPNGTTCP 326

Query: 529 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS 583
           ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSP+PSPSHLT IS
Sbjct: 327 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPNPSPSHLTPIS 381

BLAST of Lsi05G021200 vs. ExPASy TrEMBL
Match: A0A1S3AV32 (putative RING-H2 finger protein ATL21A OS=Cucumis melo OX=3656 GN=LOC103483191 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 1.2e-149
Identity = 256/295 (86.78%), Postives = 271/295 (91.86%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYDSD CLPRR LQG  KLN SNSPFIPL SQN TFLSCP QF MS FP+IGCLSNST+
Sbjct: 87  QLYDSDDCLPRRFLQGLNKLNFSNSPFIPLFSQNITFLSCPPQFTMSHFPIIGCLSNSTN 146

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           S++ATSS SF KSMSTSC IM T+ +PVSNPDE NQF TNLNG+LVLTWYSPACGICETE
Sbjct: 147 SILATSSTSFVKSMSTSCTIMTTLPVPVSNPDETNQFSTNLNGDLVLTWYSPACGICETE 206

Query: 409 GRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGY 468
           GRLCGYKSNSGQGIAC+ KYTSEENNGLRVLRIICVVI+LPTLMCV GLGCFIC AKW Y
Sbjct: 207 GRLCGYKSNSGQGIACFDKYTSEENNGLRVLRIICVVILLPTLMCVIGLGCFICMAKWSY 266

Query: 469 GLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 528
            L DGRGNQVQH+QQVNPAGSDLEAP+RLSGL+ESTIESYQKVILGESRRLPGPNGTTCP
Sbjct: 267 SLTDGRGNQVQHRQQVNPAGSDLEAPSRLSGLNESTIESYQKVILGESRRLPGPNGTTCP 326

Query: 529 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS 583
           ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSP+PSPSHLT IS
Sbjct: 327 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPNPSPSHLTPIS 381

BLAST of Lsi05G021200 vs. ExPASy TrEMBL
Match: A0A0A0L3V8 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119610 PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.8e-145
Identity = 250/295 (84.75%), Postives = 269/295 (91.19%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYDSD CLP RLLQGF K+N SNSPF+PL SQN TFLSCP +F MS FP+I CLSNST 
Sbjct: 88  QLYDSDDCLPGRLLQGFNKINFSNSPFMPLFSQNITFLSCPPRFTMSHFPIIDCLSNSTT 147

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           S++ATSS SF KSMSTSC IM T+ +PVSNPDE NQF T+LNG+LVLTW SPACGICETE
Sbjct: 148 SILATSSTSFVKSMSTSCTIMKTLPVPVSNPDETNQFSTDLNGDLVLTWDSPACGICETE 207

Query: 409 GRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGY 468
           GRLCGYKSNSGQGI+C+ KYTSEENNGLRVLRIICVVI+LPTL+CVFGLGCFIC AKW Y
Sbjct: 208 GRLCGYKSNSGQGISCFDKYTSEENNGLRVLRIICVVILLPTLICVFGLGCFICLAKWSY 267

Query: 469 GLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 528
            L DGRGNQVQH+QQVNPAGSDLEAPTRLSGL+ESTIESYQKVILGESRRLPGPNGTTC 
Sbjct: 268 SLTDGRGNQVQHRQQVNPAGSDLEAPTRLSGLNESTIESYQKVILGESRRLPGPNGTTCS 327

Query: 529 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS 583
           ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSP+PSPSHLT IS
Sbjct: 328 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPNPSPSHLTPIS 382

BLAST of Lsi05G021200 vs. ExPASy TrEMBL
Match: A0A6J1D2K0 (putative RING-H2 finger protein ATL21A OS=Momordica charantia OX=3673 GN=LOC111016421 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 8.8e-145
Identity = 249/293 (84.98%), Postives = 276/293 (94.20%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTDS 348
           +LYD +ACLPR+LL+GFK  +S+SPF+PL SQNFTFLSCPSQFA+SRFPV+GCLSNST+S
Sbjct: 86  QLYDPEACLPRKLLEGFK--ISDSPFMPLFSQNFTFLSCPSQFAISRFPVLGCLSNSTNS 145

Query: 349 VVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETEG 408
           VVATSSMSF KSMST+CKI+AT+ +PVS PDEA  F +NLNG+LVLTWYSPACGICE+EG
Sbjct: 146 VVATSSMSFVKSMSTACKIIATLPVPVSRPDEAEGFSSNLNGDLVLTWYSPACGICESEG 205

Query: 409 RLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGYG 468
           RLCGYK+++GQGI CY+ YT+EENN LRV RIICVVIVLPTLMCVFGLGCFICFAKWGYG
Sbjct: 206 RLCGYKNSNGQGITCYN-YTTEENNALRVFRIICVVIVLPTLMCVFGLGCFICFAKWGYG 265

Query: 469 LADGRGNQVQHQQQVNPAGSDLEA--PTRLSGLDESTIESYQKVILGESRRLPGPNGTTC 528
           LADGRGNQVQH+QQVNPAG+DLEA  PTRLSGLDESTIESY+KVILGESRRLPGPNG TC
Sbjct: 266 LADGRGNQVQHRQQVNPAGADLEAEPPTRLSGLDESTIESYEKVILGESRRLPGPNGITC 325

Query: 529 PICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLT 580
           PICLGEYL+KDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSPSPSPSHLT
Sbjct: 326 PICLGEYLSKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPSPSPSHLT 375

BLAST of Lsi05G021200 vs. ExPASy TrEMBL
Match: A0A6J1ICU2 (putative RING-H2 finger protein ATL21A OS=Cucurbita maxima OX=3661 GN=LOC111473771 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 1.5e-131
Identity = 229/292 (78.42%), Postives = 258/292 (88.36%), Query Frame = 0

Query: 289 RLYDSDACLPRR-LLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYD D CLP+R LLQGF  +LSNSPF+   S+NFTFLSC  QFA SRFPV+ CLSNST 
Sbjct: 88  QLYDPDVCLPKRLLLQGFNFDLSNSPFLLPFSKNFTFLSCQPQFATSRFPVLSCLSNSTS 147

Query: 349 SVVATSSMSFAKSM--STSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICE 408
           SVVATSSMS+ +SM  S SC+IMA +++PV+ PDEANQFLT+  G+L+LTW SPAC ICE
Sbjct: 148 SVVATSSMSYVRSMSKSNSCRIMANLTVPVAYPDEANQFLTDHYGDLLLTWNSPACSICE 207

Query: 409 TEGRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKW 468
           TEGRLCG+KSNSG GIAC++KY++ ENNGLR+LRIICVVI+LP+LMCVFG GCFICFA+W
Sbjct: 208 TEGRLCGFKSNSGLGIACFNKYSTGENNGLRILRIICVVIILPSLMCVFGFGCFICFARW 267

Query: 469 GYGLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTT 528
           GYG+AD R NQ QHQQQVNPA  + E P RLSGLDESTI+SYQKVILGESRRLPGPNGTT
Sbjct: 268 GYGIADSRRNQAQHQQQVNPA-LETEPPIRLSGLDESTIDSYQKVILGESRRLPGPNGTT 327

Query: 529 CPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSH 578
           CPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSPSPSPSH
Sbjct: 328 CPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPSPSPSH 378

BLAST of Lsi05G021200 vs. NCBI nr
Match: XP_038891282.1 (putative RING-H2 finger protein ATL21A [Benincasa hispida])

HSP 1 Score: 573.9 bits (1478), Expect = 1.5e-159
Identity = 276/295 (93.56%), Postives = 284/295 (96.27%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYD D CLPRRLLQGF KLNLSNSPFIPL SQN TFLSCPSQFAMSRFPVIGCLSNSTD
Sbjct: 89  QLYDPDDCLPRRLLQGFNKLNLSNSPFIPLFSQNITFLSCPSQFAMSRFPVIGCLSNSTD 148

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           SVVATSSMSFAKSMSTSCKIM T+ +PVSNPDE NQ LTNLNG+LVLTWYSPACGICETE
Sbjct: 149 SVVATSSMSFAKSMSTSCKIMNTLPVPVSNPDETNQLLTNLNGDLVLTWYSPACGICETE 208

Query: 409 GRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGY 468
           GRLCGYKSNSGQGIACY+KYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCF+CFAKWGY
Sbjct: 209 GRLCGYKSNSGQGIACYNKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFLCFAKWGY 268

Query: 469 GLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 528
           GL D RGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP
Sbjct: 269 GLMDSRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 328

Query: 529 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS 583
           ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSP+PSPSHLTSIS
Sbjct: 329 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPNPSPSHLTSIS 383

BLAST of Lsi05G021200 vs. NCBI nr
Match: XP_008437901.2 (PREDICTED: putative RING-H2 finger protein ATL21A [Cucumis melo] >KAA0048893.1 putative RING-H2 finger protein ATL21A [Cucumis melo var. makuwa] >TYK20843.1 putative RING-H2 finger protein ATL21A [Cucumis melo var. makuwa])

HSP 1 Score: 540.0 bits (1390), Expect = 2.5e-149
Identity = 256/295 (86.78%), Postives = 271/295 (91.86%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYDSD CLPRR LQG  KLN SNSPFIPL SQN TFLSCP QF MS FP+IGCLSNST+
Sbjct: 87  QLYDSDDCLPRRFLQGLNKLNFSNSPFIPLFSQNITFLSCPPQFTMSHFPIIGCLSNSTN 146

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           S++ATSS SF KSMSTSC IM T+ +PVSNPDE NQF TNLNG+LVLTWYSPACGICETE
Sbjct: 147 SILATSSTSFVKSMSTSCTIMTTLPVPVSNPDETNQFSTNLNGDLVLTWYSPACGICETE 206

Query: 409 GRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGY 468
           GRLCGYKSNSGQGIAC+ KYTSEENNGLRVLRIICVVI+LPTLMCV GLGCFIC AKW Y
Sbjct: 207 GRLCGYKSNSGQGIACFDKYTSEENNGLRVLRIICVVILLPTLMCVIGLGCFICMAKWSY 266

Query: 469 GLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 528
            L DGRGNQVQH+QQVNPAGSDLEAP+RLSGL+ESTIESYQKVILGESRRLPGPNGTTCP
Sbjct: 267 SLTDGRGNQVQHRQQVNPAGSDLEAPSRLSGLNESTIESYQKVILGESRRLPGPNGTTCP 326

Query: 529 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS 583
           ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSP+PSPSHLT IS
Sbjct: 327 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPNPSPSHLTPIS 381

BLAST of Lsi05G021200 vs. NCBI nr
Match: KGN56428.1 (hypothetical protein Csa_011440 [Cucumis sativus])

HSP 1 Score: 526.2 bits (1354), Expect = 3.7e-145
Identity = 250/295 (84.75%), Postives = 269/295 (91.19%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYDSD CLP RLLQGF K+N SNSPF+PL SQN TFLSCP +F MS FP+I CLSNST 
Sbjct: 88  QLYDSDDCLPGRLLQGFNKINFSNSPFMPLFSQNITFLSCPPRFTMSHFPIIDCLSNSTT 147

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           S++ATSS SF KSMSTSC IM T+ +PVSNPDE NQF T+LNG+LVLTW SPACGICETE
Sbjct: 148 SILATSSTSFVKSMSTSCTIMKTLPVPVSNPDETNQFSTDLNGDLVLTWDSPACGICETE 207

Query: 409 GRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGY 468
           GRLCGYKSNSGQGI+C+ KYTSEENNGLRVLRIICVVI+LPTL+CVFGLGCFIC AKW Y
Sbjct: 208 GRLCGYKSNSGQGISCFDKYTSEENNGLRVLRIICVVILLPTLICVFGLGCFICLAKWSY 267

Query: 469 GLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPNGTTCP 528
            L DGRGNQVQH+QQVNPAGSDLEAPTRLSGL+ESTIESYQKVILGESRRLPGPNGTTC 
Sbjct: 268 SLTDGRGNQVQHRQQVNPAGSDLEAPTRLSGLNESTIESYQKVILGESRRLPGPNGTTCS 327

Query: 529 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLTSIS 583
           ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSP+PSPSHLT IS
Sbjct: 328 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPNPSPSHLTPIS 382

BLAST of Lsi05G021200 vs. NCBI nr
Match: XP_022147517.1 (putative RING-H2 finger protein ATL21A [Momordica charantia])

HSP 1 Score: 523.9 bits (1348), Expect = 1.8e-144
Identity = 249/293 (84.98%), Postives = 276/293 (94.20%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTDS 348
           +LYD +ACLPR+LL+GFK  +S+SPF+PL SQNFTFLSCPSQFA+SRFPV+GCLSNST+S
Sbjct: 86  QLYDPEACLPRKLLEGFK--ISDSPFMPLFSQNFTFLSCPSQFAISRFPVLGCLSNSTNS 145

Query: 349 VVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETEG 408
           VVATSSMSF KSMST+CKI+AT+ +PVS PDEA  F +NLNG+LVLTWYSPACGICE+EG
Sbjct: 146 VVATSSMSFVKSMSTACKIIATLPVPVSRPDEAEGFSSNLNGDLVLTWYSPACGICESEG 205

Query: 409 RLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGYG 468
           RLCGYK+++GQGI CY+ YT+EENN LRV RIICVVIVLPTLMCVFGLGCFICFAKWGYG
Sbjct: 206 RLCGYKNSNGQGITCYN-YTTEENNALRVFRIICVVIVLPTLMCVFGLGCFICFAKWGYG 265

Query: 469 LADGRGNQVQHQQQVNPAGSDLEA--PTRLSGLDESTIESYQKVILGESRRLPGPNGTTC 528
           LADGRGNQVQH+QQVNPAG+DLEA  PTRLSGLDESTIESY+KVILGESRRLPGPNG TC
Sbjct: 266 LADGRGNQVQHRQQVNPAGADLEAEPPTRLSGLDESTIESYEKVILGESRRLPGPNGITC 325

Query: 529 PICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPSPSHLT 580
           PICLGEYL+KDTVRCIPECKHCFHVDCIDQWLRVNSSCP+CRNSPSPSPSHLT
Sbjct: 326 PICLGEYLSKDTVRCIPECKHCFHVDCIDQWLRVNSSCPLCRNSPSPSPSHLT 375

BLAST of Lsi05G021200 vs. NCBI nr
Match: XP_031738913.1 (putative RING-H2 finger protein ATL21A [Cucumis sativus])

HSP 1 Score: 509.2 bits (1310), Expect = 4.6e-140
Identity = 250/328 (76.22%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGF-KLNLSNSPFIPLSSQNFTFLSCPSQFAMSRFPVIGCLSNSTD 348
           +LYDSD CLP RLLQGF K+N SNSPF+PL SQN TFLSCP +F MS FP+I CLSNST 
Sbjct: 88  QLYDSDDCLPGRLLQGFNKINFSNSPFMPLFSQNITFLSCPPRFTMSHFPIIDCLSNSTT 147

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETE 408
           S++ATSS SF KSMSTSC IM T+ +PVSNPDE NQF T+LNG+LVLTW SPACGICETE
Sbjct: 148 SILATSSTSFVKSMSTSCTIMKTLPVPVSNPDETNQFSTDLNGDLVLTWDSPACGICETE 207

Query: 409 GRLCGYKSNSGQGIACYSKYT---------------------------------SEENNG 468
           GRLCGYKSNSGQGI+C+ KYT                                 SEENNG
Sbjct: 208 GRLCGYKSNSGQGISCFDKYTSGKGSALNYFLSLCLNYTIPKSSKIHLLQSVEISEENNG 267

Query: 469 LRVLRIICVVIVLPTLMCVFGLGCFICFAKWGYGLADGRGNQVQHQQQVNPAGSDLEAPT 528
           LRVLRIICVVI+LPTL+CVFGLGCFIC AKW Y L DGRGNQVQH+QQVNPAGSDLEAPT
Sbjct: 268 LRVLRIICVVILLPTLICVFGLGCFICLAKWSYSLTDGRGNQVQHRQQVNPAGSDLEAPT 327

Query: 529 RLSGLDESTIESYQKVILGESRRLPGPNGTTCPICLGEYLTKDTVRCIPECKHCFHVDCI 583
           RLSGL+ESTIESYQKVILGESRRLPGPNGTTC ICLGEYLTKDTVRCIPECKHCFHVDCI
Sbjct: 328 RLSGLNESTIESYQKVILGESRRLPGPNGTTCSICLGEYLTKDTVRCIPECKHCFHVDCI 387

BLAST of Lsi05G021200 vs. TAIR 10
Match: AT2G46495.1 (RING/U-box superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 2.1e-58
Identity = 129/291 (44.33%), Postives = 177/291 (60.82%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFA-MSRFPVIGCLSNSTD 348
           RLYD + CL R+LL     ++S SPF  L   ++TFLSCP++ A  SRF  I CL NST 
Sbjct: 90  RLYDPENCLARKLL---TFDISRSPFSALYLVSYTFLSCPNEVAKSSRFDSIPCLGNSTT 149

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVSNP--DEANQFLTNLNG-ELVLTWYSPACGIC 408
           S +AT+S+  AKSM  SC+I+ T+ +PVS     + ++F T++N  +L L W SP+C  C
Sbjct: 150 SFLATTSLDLAKSMLPSCQIVKTLDVPVSRRVIAKKSRFSTDVNDKDLWLKWDSPSCSDC 209

Query: 409 ETEGRLCGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAK 468
           E +   CG++SN+   + C+    S  N   +VL+II + I+ P  +    +   +C ++
Sbjct: 210 ERDFLRCGFRSNTSLQVKCFPFENSGYNTEPQVLKIILLSIIGPLTIFATCIAVGVCTSE 269

Query: 469 WGYGLADGRGNQVQHQQQVNPAGSDLEAPTRLSGLDESTIESYQKVILGESRRLPGPN-G 528
               L          Q+ V  A          +GLDES IESY+K  LGESRRLPG N  
Sbjct: 270 RFASLI---------QRNVAIAALQPNEVIVTTGLDESIIESYKKTELGESRRLPGNNDD 329

Query: 529 TTCPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSPS 575
             CPICL EY +K+TVRCIPEC HCFH +CID WL+++ SCP+CRNSPSP+
Sbjct: 330 IVCPICLSEYASKETVRCIPECDHCFHSECIDVWLKIHGSCPLCRNSPSPA 368

BLAST of Lsi05G021200 vs. TAIR 10
Match: AT4G29670.2 (atypical CYS HIS rich thioredoxin 2 )

HSP 1 Score: 218.0 bits (554), Expect = 2.0e-56
Identity = 99/128 (77.34%), Postives = 112/128 (87.50%), Query Frame = 0

Query: 30  QVQAAVAEKDQPKWWEKNAQNMIDIHSTQEFLTALSEAGDRLVIVEFYGTWCASCRALFP 89
           +V   VAE + PKWWE+NA NM+DIHST+EFL+ALS AG+RLVIVEFYGTWCASCRALFP
Sbjct: 84  KVDENVAETEPPKWWERNAPNMVDIHSTEEFLSALSGAGERLVIVEFYGTWCASCRALFP 143

Query: 90  RLCRTADEHPEILFLKVNFDENKPMCKSLNVKVLPYFHFYRGADGQLESFSCSLAKVSPP 149
           +LC+TA EHP+I+FLKVNFDENKPMCKSLNV+VLP+FHFYRGADGQLESFSCSLAKV   
Sbjct: 144 KLCKTAVEHPDIVFLKVNFDENKPMCKSLNVRVLPFFHFYRGADGQLESFSCSLAKVKKA 203

Query: 150 PHPHPHPQ 158
               P PQ
Sbjct: 204 ISVSPFPQ 211

BLAST of Lsi05G021200 vs. TAIR 10
Match: AT4G29670.1 (atypical CYS HIS rich thioredoxin 2 )

HSP 1 Score: 214.2 bits (544), Expect = 2.9e-55
Identity = 95/116 (81.90%), Postives = 108/116 (93.10%), Query Frame = 0

Query: 30  QVQAAVAEKDQPKWWEKNAQNMIDIHSTQEFLTALSEAGDRLVIVEFYGTWCASCRALFP 89
           +V   VAE + PKWWE+NA NM+DIHST+EFL+ALS AG+RLVIVEFYGTWCASCRALFP
Sbjct: 84  KVDENVAETEPPKWWERNAPNMVDIHSTEEFLSALSGAGERLVIVEFYGTWCASCRALFP 143

Query: 90  RLCRTADEHPEILFLKVNFDENKPMCKSLNVKVLPYFHFYRGADGQLESFSCSLAK 146
           +LC+TA EHP+I+FLKVNFDENKPMCKSLNV+VLP+FHFYRGADGQLESFSCSLAK
Sbjct: 144 KLCKTAVEHPDIVFLKVNFDENKPMCKSLNVRVLPFFHFYRGADGQLESFSCSLAK 199

BLAST of Lsi05G021200 vs. TAIR 10
Match: AT2G25410.1 (RING/U-box superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-52
Identity = 127/300 (42.33%), Postives = 170/300 (56.67%), Query Frame = 0

Query: 289 RLYDSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAM-SRFPVIGCLSNSTD 348
           RL D + CL RRLL     + S SPF  L S+N+TFL CP +  + + F  I CL N+T 
Sbjct: 93  RLNDPENCLARRLL---NFDPSGSPFSFLRSRNYTFLICPKEANITASFRAIDCLGNTTS 152

Query: 349 SVVATSSMSFAKSMSTSCKIMATISIPVS-------NPDEANQFLTNLNGELVLTWYSPA 408
           S       +   SM +SC I   + +P S        PD  N      + ++ L W SP 
Sbjct: 153 SFFVVQFENLG-SMPSSCHIFKILPLPFSWFVAYTTYPDGQN------SRDMWLKWDSPD 212

Query: 409 CGICE--TEGRLCGYKSNSGQGIACYSKYT-SEENNGLRVLRIICVVIVLPTLMCVFGLG 468
           C  CE  T  R CG+K+N+   + C+S       N GL+VL+I+C+ +V P     F +G
Sbjct: 213 CRDCERRTNSR-CGFKNNTSHQVECFSSVNPGLHNTGLQVLKIMCLSLVGPLTALTFCVG 272

Query: 469 CFICFAKWGYGLADGRGNQVQHQQQVNPAGSDLEAPTR---LSGLDESTIESYQKVILGE 528
             +C        ++   +Q+Q       +GS    P+      GLDESTIESY+KV LGE
Sbjct: 273 LVMC-------SSERVSSQIQQAVVARLSGSVTSQPSNEVARIGLDESTIESYKKVELGE 332

Query: 529 SRRLP-GPNGTTCPICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNSPSP 574
           SRRLP G N   CPICL EY TK+TVRC+PEC+HCFH +CID WL+++SSCPVCR++PSP
Sbjct: 333 SRRLPTGSNDVVCPICLSEYATKETVRCLPECEHCFHTECIDAWLKLHSSCPVCRSNPSP 374

BLAST of Lsi05G021200 vs. TAIR 10
Match: AT2G46494.1 (RING/U-box superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.7e-52
Identity = 114/283 (40.28%), Postives = 162/283 (57.24%), Query Frame = 0

Query: 292 DSDACLPRRLLQGFKLNLSNSPFIPLSSQNFTFLSCPSQFAM-SRFPVIGCLSNSTDSVV 351
           D + CL +RLL     N+S SPF P     +TFL+CP++  + S +P I CLSNST S  
Sbjct: 92  DPENCLAKRLL---TFNISGSPFSPRFDTLYTFLTCPNELVLPSWYPSIPCLSNSTSSFF 151

Query: 352 ATSSMSFAKSMSTSCKIMATISIPVSNPDEANQFLTNLNGELVLTWYSPACGICETEGRL 411
           ATS+ + A+SM  SC+I+  I +P  +P    +F + LN  L+L W SP C  CE +   
Sbjct: 152 ATSNFALAESMLPSCQIVKRIYVPADSPFAETRFSSYLNQSLLLEWNSPNCRGCEIDYLR 211

Query: 412 CGYKSNSGQGIACYSKYTSEENNGLRVLRIICVVIVLPTLMCVFGLGCFICFAKWGYGLA 471
           CG+K+ +   + C+    S   +   V  +IC+ I+   ++ V       C A     + 
Sbjct: 212 CGFKNKASPEVKCFGAKKSGHLSRAVVAVLICLSIIGAVILFV------TCIA-----IR 271

Query: 472 DGRGNQVQHQQQVNPAGSDLEAPTRL---SGLDESTIESYQKVILGESRRLPGPNGTTCP 531
                + +H      A + ++ P  +    GLD+STIE Y+ + LGESRR PG NG  CP
Sbjct: 272 IHNTPRRRHWAVPAAAATVMQQPREVMATRGLDQSTIEKYKTMELGESRRPPGTNGIVCP 331

Query: 532 ICLGEYLTKDTVRCIPECKHCFHVDCIDQWLRVNSSCPVCRNS 571
           ICL EY++K+TVR IPEC HCFH  CID WL+++ SCP+CRNS
Sbjct: 332 ICLSEYVSKETVRFIPECDHCFHAKCIDVWLKIHGSCPLCRNS 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0CH013.0e-5744.33Putative RING-H2 finger protein ATL21A OS=Arabidopsis thaliana OX=3702 GN=ATL21A... [more]
Q8LCT32.8e-5577.34Thioredoxin-like 2-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g29670... [more]
Q5TKD82.0e-5384.75Thioredoxin-like 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q9SKK81.9e-5142.33RING-H2 finger protein ATL22 OS=Arabidopsis thaliana OX=3702 GN=ATL22 PE=2 SV=2[more]
P0CH022.5e-5140.28Putative RING-H2 finger protein ATL21B OS=Arabidopsis thaliana OX=3702 GN=ATL21B... [more]
Match NameE-valueIdentityDescription
A0A5A7TZ531.2e-14986.78Putative RING-H2 finger protein ATL21A OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3AV321.2e-14986.78putative RING-H2 finger protein ATL21A OS=Cucumis melo OX=3656 GN=LOC103483191 P... [more]
A0A0A0L3V81.8e-14584.75RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119610 P... [more]
A0A6J1D2K08.8e-14584.98putative RING-H2 finger protein ATL21A OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1ICU21.5e-13178.42putative RING-H2 finger protein ATL21A OS=Cucurbita maxima OX=3661 GN=LOC1114737... [more]
Match NameE-valueIdentityDescription
XP_038891282.11.5e-15993.56putative RING-H2 finger protein ATL21A [Benincasa hispida][more]
XP_008437901.22.5e-14986.78PREDICTED: putative RING-H2 finger protein ATL21A [Cucumis melo] >KAA0048893.1 p... [more]
KGN56428.13.7e-14584.75hypothetical protein Csa_011440 [Cucumis sativus][more]
XP_022147517.11.8e-14484.98putative RING-H2 finger protein ATL21A [Momordica charantia][more]
XP_031738913.14.6e-14076.22putative RING-H2 finger protein ATL21A [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G46495.12.1e-5844.33RING/U-box superfamily protein [more]
AT4G29670.22.0e-5677.34atypical CYS HIS rich thioredoxin 2 [more]
AT4G29670.12.9e-5581.90atypical CYS HIS rich thioredoxin 2 [more]
AT2G25410.11.3e-5242.33RING/U-box superfamily protein [more]
AT2G46494.11.7e-5240.28RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 526..567
e-value: 1.8E-5
score: 34.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 525..568
e-value: 5.4E-12
score: 45.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 526..568
score: 12.282736
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 55..133
e-value: 2.9E-16
score: 59.3
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 25..164
score: 10.019888
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 488..573
e-value: 1.3E-19
score: 71.6
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 25..145
e-value: 3.8E-22
score: 80.4
NoneNo IPR availablePANTHERPTHR46279:SF2RING-H2 FINGER PROTEIN ATL21A-RELATEDcoord: 289..579
NoneNo IPR availablePANTHERPTHR46279RING/U-BOX SUPERFAMILY PROTEINcoord: 289..579
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 525..568
e-value: 1.50439E-14
score: 65.7861
NoneNo IPR availableCDDcd02947TRX_familycoord: 58..136
e-value: 1.7759E-19
score: 81.4504
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 519..572
IPR017937Thioredoxin, conserved sitePROSITEPS00194THIOREDOXIN_1coord: 73..91
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 39..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G021200.1Lsi05G021200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030247 polysaccharide binding