Lsi05G021130 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G021130
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	photosynthetic NDH subunit of lumenal location 2, chloroplastic
Location	chr05: 27995044 .. 27997534 (-)
RNA-Seq Expression	Lsi05G021130
Synteny	Lsi05G021130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGACAAAGTGATAATACGTAAGGTTGTGAATGAATGGGAAGAGATGAGAAGGTAAGGCAAGGCCCACAAAGTGTGTGTGTGTGTTTTCCCTATGGAAATGAAATGTAAGAAGCCAAAAGAGTTTTATCTTCCAAGCCATGCCTGCCTTTGCCAACGCCAACACTCTCTCCTCCTCTTGGATTTCCACTCCCCACAAATGCCGCCACCGTCGTCGCCACCATTTTCCAATTGTCACCGCCTCCCTTTCCGCCCACCGCCGCGATATTGCGACAGCCCTTGCAGTGGTGGTTGGGCTGCAGGCCGCAGCAGCACCCCTCGCACTAGCCCAAAGCTGGGGCACGCGCTCGTTCATTAAGGAGCGATACTTTGAGCCCGGGCTATCGGCGGAGGAGGCGGTGGCGAGGATAAGGCAGACCGCGGAAGGACTTCACAGCATTCGTGAGATGCTGGAAACCATGTCATGGAGATATGTGATATTCTACATTCGTTTGAAGTCGGCTTATCTATCACAAGACTTGAAAACTGCCATGTCTGTTTTGCCACAAGCTCGCCAAAGTGATTTTGTTAAGACTGCTAATGAGTTGGTCGATAACATGGCTGAGGTATGTACTCTTTGTTTCGCGTTTCACATGTTTCATAACGTTTAAAATACAACGTAAATGTAAATATAAATGTTAAATGTAGGATCTTAAACGTATAGGAAAATTAAAAATTACACTTTGAGTTCTTACCTGAAGGAGATTTTGATATTCTAAAACGTGTTACGTTTGATTTTATTTGTGTTTTGATTATTAATTAGAGAAAATTGTTTGAATCCTATCTCGAAATTGGAATATTTTTTTTTTGGAAAACTACCTTTTTGATTTTTAGATTTTGGTTTAGTTTTCATTTGGTCCATAAATTTCAAAATATTACATTTTTAGTTCTTTTAAGTTTTGAGTTTCATTTCAATTGAATTTTTAGATTTCAAAATATTACAATTTTACCAATTGAGATTTGAGTATTGTTTTAATTTGATCCCTATGTTTCAAAATTTACACTTTTAGCCTTTATTTTTTACTAAATATTTATTTCAATATTTGGCGCTAATATCTATCCATTAATTTAAAATAATTATGAAGTGAAATTTTAAAATCAATTTTAACAAATTAATTAATTATAAGGAAAATTTTTACAAATAAAAAAAAATGTCAAACTATTTACATAAAATAGCAAAAAAATACTGATAGACATTGATAGACTTCTATCAGCATCTATCAGTGATAGACTTCTATCATTTCTATCACTGATAGATCCTGATAGACTTCTATCAGCGTCTATCACAACTATTTAAAATTTTTGCTATTTTGTGTAAATAGTTTACCTTATTTTTCTATTTTTAAAACTTTCCCCAATTATAATTCTTTGAAATTAATTAATAGATATTAACATTGAATATTAAAAAAAGTGCGTATTTAAGGATAATTATTCAAGGTTAAAAGTGTAAAATTTTGTAACTAAGGATCAAATTGTAACAAAACTCAAATCTCAAAGGTAAAAGTGTACCATTTTGAAACTTACAGACTAAATTGAAATCAAATTCAAAAGTTAAAAGGACTAAAATTATAACATTTTGAAACATAAAAACCAAATAGAAACTAAATCAAAAACTTAAAGGCTAAAAATGTATTTTTTTTTCTGATAAATATATATATATATATATATATTAAACAAAGTATGCTTTAGAATCTTTGAGGAAAGTATGCTTTAGAATCTGTGAGATTGTTGGAATTTAACATGGGTAATATAACTATTTTACTCTAGTTTATATAAATTCAAGAATATATGTAATAGTAAGATGTCCTGCTGACATTCTAATATAGTTTTATTTTAATAATTCCAAAATATGTGTGGATATTTTAAAATCTATGAAATGCAATAAAAATAGACAAAATAGATAAACAACGTTGAAATGTCAATAAGTGTTTTACTTGAGGTCTAGGTAGGCAAGTATATATTGTCCTAAATTTAGTCCATAGTCTAATTGATTAGTTTGATTTTGTGATTGCCATTCAACTTTGGATTTGGATTGATGTTTCAGTTGGATTATTACGTTCGCACGCCGAAGGTGTATGAGTCTTATTTGTATTATGAAAAGACATTGAAATCTATTGATGATCTGGTGGCTTTACTAGCATAGCTGCAAATAATATCTAACAATCTTCATCACAGATTGAATTAATGCATCAAAAGGAAGACTTGATCTTACGAGATTTTGATGTGTTGTGTTAAGATAATTTGGTAGTCTTTTATTATATAAATACATGTGTAAAAAATTTATTTGACAATAGTAATTAATCTAGAAATTATTTCTTCCATTTTCAAAAATTGAATACTTTTGAGGTTCTTGGATAACTTGGTTATATTTTTAAATTGTAACTGAGTCATTTGTCAATGATGAATGGGATTGAGAATTAGGAAGAGAATTGAACAAGTGTTATCTTGTAATGAG

mRNA sequence

GGACAAAGTGATAATACGTAAGGTTGTGAATGAATGGGAAGAGATGAGAAGGTAAGGCAAGGCCCACAAAGTGTGTGTGTGTGTTTTCCCTATGGAAATGAAATGTAAGAAGCCAAAAGAGTTTTATCTTCCAAGCCATGCCTGCCTTTGCCAACGCCAACACTCTCTCCTCCTCTTGGATTTCCACTCCCCACAAATGCCGCCACCGTCGTCGCCACCATTTTCCAATTGTCACCGCCTCCCTTTCCGCCCACCGCCGCGATATTGCGACAGCCCTTGCAGTGGTGGTTGGGCTGCAGGCCGCAGCAGCACCCCTCGCACTAGCCCAAAGCTGGGGCACGCGCTCGTTCATTAAGGAGCGATACTTTGAGCCCGGGCTATCGGCGGAGGAGGCGGTGGCGAGGATAAGGCAGACCGCGGAAGGACTTCACAGCATTCGTGAGATGCTGGAAACCATGTCATGGAGATATGTGATATTCTACATTCGTTTGAAGTCGGCTTATCTATCACAAGACTTGAAAACTGCCATGTCTGTTTTGCCACAAGCTCGCCAAAGTGATTTTGTTAAGACTGCTAATGAGTTGGTCGATAACATGGCTGAGTTGGATTATTACGTTCGCACGCCGAAGGTGTATGAGTCTTATTTGTATTATGAAAAGACATTGAAATCTATTGATGATCTGGTGGCTTTACTAGCATAGCTGCAAATAATATCTAACAATCTTCATCACAGATTGAATTAATGCATCAAAAGGAAGACTTGATCTTACGAGATTTTGATGTGTTGTGTTAAGATAATTTGGTAGTCTTTTATTATATAAATACATGTGTAAAAAATTTATTTGACAATAGTAATTAATCTAGAAATTATTTCTTCCATTTTCAAAAATTGAATACTTTTGAGGTTCTTGGATAACTTGGTTATATTTTTAAATTGTAACTGAGTCATTTGTCAATGATGAATGGGATTGAGAATTAGGAAGAGAATTGAACAAGTGTTATCTTGTAATGAG

Coding sequence (CDS)

ATGCCTGCCTTTGCCAACGCCAACACTCTCTCCTCCTCTTGGATTTCCACTCCCCACAAATGCCGCCACCGTCGTCGCCACCATTTTCCAATTGTCACCGCCTCCCTTTCCGCCCACCGCCGCGATATTGCGACAGCCCTTGCAGTGGTGGTTGGGCTGCAGGCCGCAGCAGCACCCCTCGCACTAGCCCAAAGCTGGGGCACGCGCTCGTTCATTAAGGAGCGATACTTTGAGCCCGGGCTATCGGCGGAGGAGGCGGTGGCGAGGATAAGGCAGACCGCGGAAGGACTTCACAGCATTCGTGAGATGCTGGAAACCATGTCATGGAGATATGTGATATTCTACATTCGTTTGAAGTCGGCTTATCTATCACAAGACTTGAAAACTGCCATGTCTGTTTTGCCACAAGCTCGCCAAAGTGATTTTGTTAAGACTGCTAATGAGTTGGTCGATAACATGGCTGAGTTGGATTATTACGTTCGCACGCCGAAGGTGTATGAGTCTTATTTGTATTATGAAAAGACATTGAAATCTATTGATGATCTGGTGGCTTTACTAGCATAG

Protein sequence

MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPLALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLVALLA

Homology

[Close]

BLAST of Lsi05G021130 vs. ExPASy Swiss-Prot
Match: Q9XI73 (Photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PNSL2 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.1e-48
Identity = 97/148 (65.54%), Postives = 119/148 (80.41%), Query Frame = 0


Query: 40  RRDIATALAVVVGLQAAAAPLALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHS 99
           RR   T+L     +  A +     + WGTRSFIKE+YF PGLS E+A ARI+QTAEGL  
Sbjct: 43  RRRTLTSLITFTVIGGATSSALAQEKWGTRSFIKEKYFMPGLSPEDAAARIKQTAEGLRD 102

Query: 100 IREMLETMSWRYVIFYIRLKSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYY 159
           +REML+ MSWRYVIFYIRLK AYLSQDL  AM++LP++R++D+V+ ANELV+NM+ELD+Y
Sbjct: 103 MREMLDHMSWRYVIFYIRLKQAYLSQDLTNAMNILPESRRNDYVQAANELVENMSELDFY 162

Query: 160 VRTPKVYESYLYYEKTLKSIDDLVALLA 188
           VRTPKVYESYLYYEKTLKSID++V  LA
Sbjct: 163 VRTPKVYESYLYYEKTLKSIDNVVEFLA 190

[Close]

BLAST of Lsi05G021130 vs. ExPASy Swiss-Prot
Match: Q41806 (Oxygen-evolving enhancer protein 3-2, chloroplastic OS=Zea mays OX=4577 GN=PSBQ2 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.7e-09
Identity = 34/122 (27.87%), Postives = 69/122 (56.56%), Query Frame = 0


Query: 68  TRSF---IKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYLS 127
           TR F   +KER++   L   EAVAR++ +A+ + +++ +++  +W YV   +RL+++YL 
Sbjct: 91  TRDFDLPLKERFYLQPLPPAEAVARVKTSAQDIINLKPLIDKKAWPYVQNDLRLRASYLR 150

Query: 128 QDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLVA 187
            DLKT ++  P+  +    +   +L   + +LD+  +     E+  Y+  T  ++ D++A
Sbjct: 151 YDLKTVIASKPKEEKKSLKELTGKLFSTIDDLDHAAKMKSTPEAEKYFAATKDALGDVLA 210

[Close]

BLAST of Lsi05G021130 vs. ExPASy Swiss-Prot
Match: Q41048 (Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Zea mays OX=4577 GN=PSBQ1 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-08
Identity = 30/115 (26.09%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 72  IKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYLSQDLKTAM 131
           +KER++   L   EA AR++ +A+ + +++ +++  +W YV   +RL+++YL  DLKT +
Sbjct: 102 LKERFYLQPLPPAEAAARVKTSAQDIINLKPLIDKKAWPYVQNDLRLRASYLRYDLKTVI 161

Query: 132 SVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLVALL 187
           +  P+  +    +   +L   + +LD+  +     E+  Y+  T  ++ D++A L
Sbjct: 162 ASKPKEEKKSLKELTGKLFSTIDDLDHAAKIKSTPEAEKYFAATKDALGDVLAKL 216

[Close]

BLAST of Lsi05G021130 vs. ExPASy Swiss-Prot
Match: Q9XFT3 (Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSBQ1 PE=1 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 4.1e-08
Identity = 39/150 (26.00%), Postives = 79/150 (52.67%), Query Frame = 0


Query: 43  IATALAVVVGLQAAAA---PLALAQSWGTRSF---IKERYFEPGLSAEEAVARIRQTAEG 102
           +A A+++ VG   A +   P     S   R F   +K+R++   L   EA AR +++A+ 
Sbjct: 74  LADAISIKVGPPPAPSGGLPAGTDNSDQARDFALALKDRFYLQPLPPTEAAARAKESAKD 133

Query: 103 LHSIREMLETMSWRYVIFYIRLKSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAEL 162
           + +++ +++  +W YV   +R K++YL  DL T +S  P+  +        +L D +  L
Sbjct: 134 IINVKPLIDRKAWPYVQNDLRSKASYLRYDLNTIISSKPKDEKKSLKDLTTKLFDTIDNL 193

Query: 163 DYYVRTPKVYESYLYYEKTLKSIDDLVALL 187
           DY  +     ++  YY +T+ ++++++A L
Sbjct: 194 DYAAKKKSPSQAEKYYAETVSALNEVLAKL 223

[Close]

BLAST of Lsi05G021130 vs. ExPASy Swiss-Prot
Match: P12301 (Oxygen-evolving enhancer protein 3, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSBQ PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.1e-08
Identity = 30/120 (25.00%), Postives = 67/120 (55.83%), Query Frame = 0


Query: 67  GTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYLSQD 126
           GT  + K+R++   L   EA  R + +A  + ++++ ++  +W  +   +RL+++YL  D
Sbjct: 112 GTLPYTKDRFYLQPLPPTEAAQRAKVSASEILNVKQFIDRKAWPSLQNDLRLRASYLRYD 171

Query: 127 LKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLVALL 186
           LKT +S  P+  +    +  ++L  ++  LD+  +     E+  YY +T+ +I++++A L
Sbjct: 172 LKTVISAKPKDEKKSLQELTSKLFSSIDNLDHAAKIKSPTEAEKYYGQTVSNINEVLAKL 231

[Close]

BLAST of Lsi05G021130 vs. ExPASy TrEMBL
Match: A0A6J1G2K7 (photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111450221 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.5e-79
Identity = 161/187 (86.10%), Postives = 172/187 (91.98%), Query Frame = 0


Query: 1   MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPL 60
           MPAFANA TL SS I TPH+C    RH FPI++ASLS HRR IATALA+VVGLQAAA P 
Sbjct: 1   MPAFANATTLFSSRIPTPHRC---HRHPFPIISASLSIHRRHIATALALVVGLQAAAPP- 60

Query: 61  ALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKS 120
           ALAQ+WGT SFIKERYFEPGLS EEAVARIRQTAEGLHSIR+MLETMSWRYVIFYIRLKS
Sbjct: 61  ALAQTWGTHSFIKERYFEPGLSGEEAVARIRQTAEGLHSIRDMLETMSWRYVIFYIRLKS 120

Query: 121 AYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180
           AYLSQDLKTAMSVLPQARQ+++VKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS+D
Sbjct: 121 AYLSQDLKTAMSVLPQARQNEYVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSMD 180

Query: 181 DLVALLA 188
           DLVA+LA
Sbjct: 181 DLVAILA 183

[Close]

BLAST of Lsi05G021130 vs. ExPASy TrEMBL
Match: A0A6J1I9U1 (photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111471370 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 3.0e-78
Identity = 159/187 (85.03%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 1   MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPL 60
           MPAFANA TL SS I TPH+C    RH FPI++ASLS  RR IATALA+VVGLQAAA P 
Sbjct: 1   MPAFANATTLFSSRIPTPHRC---HRHPFPIISASLSIQRRHIATALALVVGLQAAAPP- 60

Query: 61  ALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKS 120
           ALAQ+WGT SFIKERYF+PGLS EEAVARIRQTAEGLHSIR+MLETMSWRYVIFYIRLKS
Sbjct: 61  ALAQTWGTHSFIKERYFQPGLSGEEAVARIRQTAEGLHSIRDMLETMSWRYVIFYIRLKS 120

Query: 121 AYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180
           AYLSQDLKTAMSVLPQARQ+D+VKTANELVDNMAELDYY+RTPKVYESYLYYEKTLKS+D
Sbjct: 121 AYLSQDLKTAMSVLPQARQNDYVKTANELVDNMAELDYYIRTPKVYESYLYYEKTLKSMD 180

Query: 181 DLVALLA 188
           DLVA+LA
Sbjct: 181 DLVAILA 183

[Close]

BLAST of Lsi05G021130 vs. ExPASy TrEMBL
Match: A0A0A0L3W3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119660 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 6.6e-78
Identity = 161/189 (85.19%), Postives = 169/189 (89.42%), Query Frame = 0


Query: 1   MPAFANA--NTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAA 60
           MP  ANA  NTL SS ISTPHK RH  R HFP++TASL  HRR IAT LA+VVGLQAA  
Sbjct: 1   MPTLANANPNTLFSSRISTPHKSRH--RPHFPVITASLPIHRRHIATTLALVVGLQAATP 60

Query: 61  PLALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRL 120
            +ALAQSWGT SFIKERYFEPGLSAEEAVARI+QTAEGLHSIREMLET SWRYVIFYIRL
Sbjct: 61  LVALAQSWGTHSFIKERYFEPGLSAEEAVARIKQTAEGLHSIREMLETTSWRYVIFYIRL 120

Query: 121 KSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS 180
           KSAYLSQDLKTAM+ LPQAR++DFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS
Sbjct: 121 KSAYLSQDLKTAMTTLPQARRNDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS 180

Query: 181 IDDLVALLA 188
           IDDLVALLA
Sbjct: 181 IDDLVALLA 187

[Close]

BLAST of Lsi05G021130 vs. ExPASy TrEMBL
Match: A0A5D3DC39 (Photosynthetic NDH subunit of lumenal location 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G001130 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.5e-77
Identity = 162/184 (88.04%), Postives = 168/184 (91.30%), Query Frame = 0


Query: 5   ANAN-TLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPLALA 64
           ANAN TL SS ISTPHK  HRR  HFPI+TASL  HRR IAT LAVV+GLQ AA PLALA
Sbjct: 6   ANANSTLFSSPISTPHKSLHRR--HFPIITASLPIHRRHIATTLAVVIGLQ-AATPLALA 65

Query: 65  QSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYL 124
           QSWGT SFIKERYFEPGLSA+EAVARI+QTAEGLHSIREMLET SWRYVIFYIRLKSAYL
Sbjct: 66  QSWGTHSFIKERYFEPGLSADEAVARIKQTAEGLHSIREMLETTSWRYVIFYIRLKSAYL 125

Query: 125 SQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLV 184
           SQDLKTAM+ LPQARQ+DFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLV
Sbjct: 126 SQDLKTAMTTLPQARQNDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLV 185

Query: 185 ALLA 188
           ALLA
Sbjct: 186 ALLA 186

[Close]

BLAST of Lsi05G021130 vs. ExPASy TrEMBL
Match: A0A1S3AVQ2 (photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483197 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.5e-77
Identity = 162/184 (88.04%), Postives = 168/184 (91.30%), Query Frame = 0


Query: 5   ANAN-TLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPLALA 64
           ANAN TL SS ISTPHK  HRR  HFPI+TASL  HRR IAT LAVV+GLQ AA PLALA
Sbjct: 6   ANANSTLFSSPISTPHKSLHRR--HFPIITASLPIHRRHIATTLAVVIGLQ-AATPLALA 65

Query: 65  QSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYL 124
           QSWGT SFIKERYFEPGLSA+EAVARI+QTAEGLHSIREMLET SWRYVIFYIRLKSAYL
Sbjct: 66  QSWGTHSFIKERYFEPGLSADEAVARIKQTAEGLHSIREMLETTSWRYVIFYIRLKSAYL 125

Query: 125 SQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLV 184
           SQDLKTAM+ LPQARQ+DFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLV
Sbjct: 126 SQDLKTAMTTLPQARQNDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLV 185

Query: 185 ALLA 188
           ALLA
Sbjct: 186 ALLA 186

[Close]

BLAST of Lsi05G021130 vs. NCBI nr
Match: XP_038880767.1 (photosynthetic NDH subunit of lumenal location 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 327.0 bits (837), Expect = 1.0e-85
Identity = 171/187 (91.44%), Postives = 176/187 (94.12%), Query Frame = 0


Query: 1   MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPL 60
           M +FANANTL  S ISTPHKC HRRRHHFPI+TASLS HRR IATALA V+GLQ A APL
Sbjct: 1   MTSFANANTLFISRISTPHKCSHRRRHHFPIITASLSIHRRHIATALAAVIGLQ-ATAPL 60

Query: 61  ALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKS 120
           ALAQSWGT SFIKERYFEPGLSAEEAVARI+QTAEGLHSIREMLETMSWRYVIFYIRLKS
Sbjct: 61  ALAQSWGTHSFIKERYFEPGLSAEEAVARIKQTAEGLHSIREMLETMSWRYVIFYIRLKS 120

Query: 121 AYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180
           AYLSQDLKTAMSVLPQARQ+DFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID
Sbjct: 121 AYLSQDLKTAMSVLPQARQNDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180

Query: 181 DLVALLA 188
           DLVALLA
Sbjct: 181 DLVALLA 186

[Close]

BLAST of Lsi05G021130 vs. NCBI nr
Match: XP_022946003.1 (photosynthetic NDH subunit of lumenal location 2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 304.3 bits (778), Expect = 7.3e-79
Identity = 161/187 (86.10%), Postives = 172/187 (91.98%), Query Frame = 0


Query: 1   MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPL 60
           MPAFANA TL SS I TPH+C    RH FPI++ASLS HRR IATALA+VVGLQAAA P 
Sbjct: 1   MPAFANATTLFSSRIPTPHRC---HRHPFPIISASLSIHRRHIATALALVVGLQAAAPP- 60

Query: 61  ALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKS 120
           ALAQ+WGT SFIKERYFEPGLS EEAVARIRQTAEGLHSIR+MLETMSWRYVIFYIRLKS
Sbjct: 61  ALAQTWGTHSFIKERYFEPGLSGEEAVARIRQTAEGLHSIRDMLETMSWRYVIFYIRLKS 120

Query: 121 AYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180
           AYLSQDLKTAMSVLPQARQ+++VKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS+D
Sbjct: 121 AYLSQDLKTAMSVLPQARQNEYVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSMD 180

Query: 181 DLVALLA 188
           DLVA+LA
Sbjct: 181 DLVAILA 183

[Close]

BLAST of Lsi05G021130 vs. NCBI nr
Match: XP_022972860.1 (photosynthetic NDH subunit of lumenal location 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 301.2 bits (770), Expect = 6.2e-78
Identity = 159/187 (85.03%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 1   MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPL 60
           MPAFANA TL SS I TPH+C    RH FPI++ASLS  RR IATALA+VVGLQAAA P 
Sbjct: 1   MPAFANATTLFSSRIPTPHRC---HRHPFPIISASLSIQRRHIATALALVVGLQAAAPP- 60

Query: 61  ALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKS 120
           ALAQ+WGT SFIKERYF+PGLS EEAVARIRQTAEGLHSIR+MLETMSWRYVIFYIRLKS
Sbjct: 61  ALAQTWGTHSFIKERYFQPGLSGEEAVARIRQTAEGLHSIRDMLETMSWRYVIFYIRLKS 120

Query: 121 AYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180
           AYLSQDLKTAMSVLPQARQ+D+VKTANELVDNMAELDYY+RTPKVYESYLYYEKTLKS+D
Sbjct: 121 AYLSQDLKTAMSVLPQARQNDYVKTANELVDNMAELDYYIRTPKVYESYLYYEKTLKSMD 180

Query: 181 DLVALLA 188
           DLVA+LA
Sbjct: 181 DLVAILA 183

[Close]

BLAST of Lsi05G021130 vs. NCBI nr
Match: KAG6598987.1 (Photosynthetic NDH subunit of lumenal location 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011082.1 Photosynthetic NDH subunit of lumenal location 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 300.8 bits (769), Expect = 8.0e-78
Identity = 160/187 (85.56%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 1   MPAFANANTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAAPL 60
           MPAFANA TL SS I TPH+C    RH FPI++ASLS  RR IATALA+VVGLQAAA P 
Sbjct: 1   MPAFANATTLFSSRIPTPHRC---HRHPFPIISASLSISRRHIATALALVVGLQAAAPP- 60

Query: 61  ALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKS 120
           ALAQ+WGT SFIKERYFEPGLS EEAVARIRQTAEGLHSIR+MLETMSWRYVIFYIRLKS
Sbjct: 61  ALAQTWGTHSFIKERYFEPGLSGEEAVARIRQTAEGLHSIRDMLETMSWRYVIFYIRLKS 120

Query: 121 AYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSID 180
           AYLSQDLKTAMSVLPQARQ+++VKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS+D
Sbjct: 121 AYLSQDLKTAMSVLPQARQNEYVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSMD 180

Query: 181 DLVALLA 188
           DLVA+LA
Sbjct: 181 DLVAILA 183

[Close]

BLAST of Lsi05G021130 vs. NCBI nr
Match: XP_004133817.1 (photosynthetic NDH subunit of lumenal location 2, chloroplastic [Cucumis sativus] >KGN56433.1 hypothetical protein Csa_010782 [Cucumis sativus])

HSP 1 Score: 300.1 bits (767), Expect = 1.4e-77
Identity = 161/189 (85.19%), Postives = 169/189 (89.42%), Query Frame = 0


Query: 1   MPAFANA--NTLSSSWISTPHKCRHRRRHHFPIVTASLSAHRRDIATALAVVVGLQAAAA 60
           MP  ANA  NTL SS ISTPHK RH  R HFP++TASL  HRR IAT LA+VVGLQAA  
Sbjct: 1   MPTLANANPNTLFSSRISTPHKSRH--RPHFPVITASLPIHRRHIATTLALVVGLQAATP 60

Query: 61  PLALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRL 120
            +ALAQSWGT SFIKERYFEPGLSAEEAVARI+QTAEGLHSIREMLET SWRYVIFYIRL
Sbjct: 61  LVALAQSWGTHSFIKERYFEPGLSAEEAVARIKQTAEGLHSIREMLETTSWRYVIFYIRL 120

Query: 121 KSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS 180
           KSAYLSQDLKTAM+ LPQAR++DFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS
Sbjct: 121 KSAYLSQDLKTAMTTLPQARRNDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKS 180

Query: 181 IDDLVALLA 188
           IDDLVALLA
Sbjct: 181 IDDLVALLA 187

[Close]

BLAST of Lsi05G021130 vs. TAIR 10
Match: AT1G14150.1 (PsbQ-like 2 )

HSP 1 Score: 194.5 bits (493), Expect = 7.6e-50
Identity = 97/148 (65.54%), Postives = 119/148 (80.41%), Query Frame = 0


Query: 40  RRDIATALAVVVGLQAAAAPLALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHS 99
           RR   T+L     +  A +     + WGTRSFIKE+YF PGLS E+A ARI+QTAEGL  
Sbjct: 43  RRRTLTSLITFTVIGGATSSALAQEKWGTRSFIKEKYFMPGLSPEDAAARIKQTAEGLRD 102

Query: 100 IREMLETMSWRYVIFYIRLKSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAELDYY 159
           +REML+ MSWRYVIFYIRLK AYLSQDL  AM++LP++R++D+V+ ANELV+NM+ELD+Y
Sbjct: 103 MREMLDHMSWRYVIFYIRLKQAYLSQDLTNAMNILPESRRNDYVQAANELVENMSELDFY 162

Query: 160 VRTPKVYESYLYYEKTLKSIDDLVALLA 188
           VRTPKVYESYLYYEKTLKSID++V  LA
Sbjct: 163 VRTPKVYESYLYYEKTLKSIDNVVEFLA 190

[Close]

BLAST of Lsi05G021130 vs. TAIR 10
Match: AT1G14150.2 (PsbQ-like 2 )

HSP 1 Score: 141.7 bits (356), Expect = 5.8e-34
Identity = 70/117 (59.83%), Postives = 90/117 (76.92%), Query Frame = 0


Query: 40  RRDIATALAVVVGLQAAAAPLALAQSWGTRSFIKERYFEPGLSAEEAVARIRQTAEGLHS 99
           RR   T+L     +  A +     + WGTRSFIKE+YF PGLS E+A ARI+QTAEGL  
Sbjct: 43  RRRTLTSLITFTVIGGATSSALAQEKWGTRSFIKEKYFMPGLSPEDAAARIKQTAEGLRD 102

Query: 100 IREMLETMSWRYVIFYIRLKSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAEL 157
           +REML+ MSWRYVIFYIRLK AYLSQDL  AM++LP++R++D+V+ ANELV+NM+E+
Sbjct: 103 MREMLDHMSWRYVIFYIRLKQAYLSQDLTNAMNILPESRRNDYVQAANELVENMSEV 159

[Close]

BLAST of Lsi05G021130 vs. TAIR 10
Match: AT4G21280.1 (photosystem II subunit QA )

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-09
Identity = 30/115 (26.09%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 72  IKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYLSQDLKTAM 131
           +K+R++   L   EA AR +++A+ + +++ +++  +W YV   +R K++YL  DL T +
Sbjct: 108 LKDRFYLQPLPPTEAAARAKESAKDIINVKPLIDRKAWPYVQNDLRSKASYLRYDLNTII 167

Query: 132 SVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLVALL 187
           S  P+  +        +L D +  LDY  +     ++  YY +T+ ++++++A L
Sbjct: 168 SSKPKDEKKSLKDLTTKLFDTIDNLDYAAKKKSPSQAEKYYAETVSALNEVLAKL 222

[Close]

BLAST of Lsi05G021130 vs. TAIR 10
Match: AT4G21280.2 (photosystem II subunit QA )

HSP 1 Score: 59.7 bits (143), Expect = 2.9e-09
Identity = 39/150 (26.00%), Postives = 79/150 (52.67%), Query Frame = 0


Query: 43  IATALAVVVGLQAAAA---PLALAQSWGTRSF---IKERYFEPGLSAEEAVARIRQTAEG 102
           +A A+++ VG   A +   P     S   R F   +K+R++   L   EA AR +++A+ 
Sbjct: 74  LADAISIKVGPPPAPSGGLPAGTDNSDQARDFALALKDRFYLQPLPPTEAAARAKESAKD 133

Query: 103 LHSIREMLETMSWRYVIFYIRLKSAYLSQDLKTAMSVLPQARQSDFVKTANELVDNMAEL 162
           + +++ +++  +W YV   +R K++YL  DL T +S  P+  +        +L D +  L
Sbjct: 134 IINVKPLIDRKAWPYVQNDLRSKASYLRYDLNTIISSKPKDEKKSLKDLTTKLFDTIDNL 193

Query: 163 DYYVRTPKVYESYLYYEKTLKSIDDLVALL 187
           DY  +     ++  YY +T+ ++++++A L
Sbjct: 194 DYAAKKKSPSQAEKYYAETVSALNEVLAKL 223

[Close]

BLAST of Lsi05G021130 vs. TAIR 10
Match: AT3G01440.1 (PsbQ-like 1 )

HSP 1 Score: 50.1 bits (118), Expect = 2.3e-06
Identity = 30/121 (24.79%), Postives = 60/121 (49.59%), Query Frame = 0


Query: 67  GTRSFIKERYFEPGLSAEEAVARIRQTAEGLHSIREMLETMSWRYVIFYIRLKSAYLSQD 126
           GTR+FIK+  +   +  +  + R+++ A  L ++ +++   +  YV  Y+RLKS +L  D
Sbjct: 100 GTRTFIKKGVYVADIGTKGRMYRVKKNAFDLLAMEDLIGPDTLNYVKKYLRLKSTFLFYD 159

Query: 127 LKTAMSVLPQARQSDFVKTANELVDNMAELDYYVRTPKVYESYLYYEKTLKSIDDLVALL 186
               +S      +      AN L DN  +L+   +T  + E+   Y+ T   + +++  +
Sbjct: 160 FDNLISAAASEDKQPLTDLANRLFDNFEKLEDAAKTKNLAETESCYKDTKFLLQEVMTRM 219

Query: 187 A 188
           A
Sbjct: 220 A 220

The following BLAST results are available for this feature:

BLAST of Lsi05G021130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XI73	1.1e-48	65.54	Photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Arabidopsis t...	[more]
Q41806	3.7e-09	27.87	Oxygen-evolving enhancer protein 3-2, chloroplastic OS=Zea mays OX=4577 GN=PSBQ2...	[more]
Q41048	1.8e-08	26.09	Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Zea mays OX=4577 GN=PSBQ1...	[more]
Q9XFT3	4.1e-08	26.00	Oxygen-evolving enhancer protein 3-1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
P12301	9.1e-08	25.00	Oxygen-evolving enhancer protein 3, chloroplastic OS=Spinacia oleracea OX=3562 G...	[more]

BLAST of Lsi05G021130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G2K7	3.5e-79	86.10	photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Cucurbita mos...	[more]
A0A6J1I9U1	3.0e-78	85.03	photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Cucurbita max...	[more]
A0A0A0L3W3	6.6e-78	85.19	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119660 PE=3 SV=1	[more]
A0A5D3DC39	2.5e-77	88.04	Photosynthetic NDH subunit of lumenal location 2 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3AVQ2	2.5e-77	88.04	photosynthetic NDH subunit of lumenal location 2, chloroplastic OS=Cucumis melo ...	[more]

BLAST of Lsi05G021130 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880767.1	1.0e-85	91.44	photosynthetic NDH subunit of lumenal location 2, chloroplastic [Benincasa hispi...	[more]
XP_022946003.1	7.3e-79	86.10	photosynthetic NDH subunit of lumenal location 2, chloroplastic [Cucurbita mosch...	[more]
XP_022972860.1	6.2e-78	85.03	photosynthetic NDH subunit of lumenal location 2, chloroplastic [Cucurbita maxim...	[more]
KAG6598987.1	8.0e-78	85.56	Photosynthetic NDH subunit of lumenal location 2, chloroplastic, partial [Cucurb...	[more]
XP_004133817.1	1.4e-77	85.19	photosynthetic NDH subunit of lumenal location 2, chloroplastic [Cucumis sativus...	[more]

BLAST of Lsi05G021130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14150.1	7.6e-50	65.54	PsbQ-like 2	[more]
AT1G14150.2	5.8e-34	59.83	PsbQ-like 2	[more]
AT4G21280.1	1.3e-09	26.09	photosystem II subunit QA	[more]
AT4G21280.2	2.9e-09	26.00	photosystem II subunit QA	[more]
AT3G01440.1	2.3e-06	24.79	PsbQ-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008797	Oxygen-evolving enhancer protein 3	PFAM	PF05757	PsbQ	coord: 20..187 e-value: 4.8E-36 score: 124.4
IPR008797	Oxygen-evolving enhancer protein 3	PANTHER	PTHR33399	OXYGEN-EVOLVING ENHANCER PROTEIN 3-1, CHLOROPLASTIC	coord: 1..187
IPR023222	PsbQ-like domain superfamily	GENE3D	1.20.120.290		coord: 45..186 e-value: 1.7E-39 score: 137.2
IPR023222	PsbQ-like domain superfamily	SUPERFAMILY	101112	Oxygen-evolving enhancer protein 3,	coord: 76..187
None	No IPR available	PANTHER	PTHR33399:SF5	PHOTOSYNTHETIC NDH SUBUNIT OF LUMENAL LOCATION 2, CHLOROPLASTIC	coord: 1..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G021130.1	Lsi05G021130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009767	photosynthetic electron transport chain
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0019898	extrinsic component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009654	photosystem II oxygen evolving complex
cellular_component	GO:0009523	photosystem II
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0045156	electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity