Lsi05G020690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G020690
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWRKY transcription factor 22
Locationchr05: 27610451 .. 27611719 (+)
RNA-Seq ExpressionLsi05G020690
SyntenyLsi05G020690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAATAGAAAGAAAAAGAAGCTGATCAAGAAGCTGAGACAAAGGTACAGATTCTCAGAGCAGAGTTCTGTCTTTGATAATGGAAGTCGATTGGGATCTTCACGCCGTCGTCAGAGGCTACTCCGCCGCGCCCTCCGCTGCCACCATTATTCCTTCTTCTTCTTCTTCTGTTTCTTCGTCTTCCAATAACTCTATCCCTTTTTCTTTTGGTAGAGACCTAACCAACCAAACAAAAAACCATTTCTTTTCCCTTCAAGATCCATTTCAACCCCCTAATTGTAATTCCACTGAGGAATTGCATGAGCTTTTCAAACCCTTTTTCCCCAAATCTCAACCCTCTCCTCCGCCGCTGCCTCCATCGCCTGCTCTGCCGCTTCTTTCTTCTCCGGCTGCTAAAATTCTGACCCATCAGAAGCAACAACAGAGCACCCATCTCCCCAAACAGCTCCATTCAGCCCCTGTTTCTGCTCCGAGATCCAAACGCCGGTGAGGAAGATTCTTCCATTTCTTCATTCTGGAAGTAGTTTTTATTTAAACTAATCGATTTTTTGTTTTGTTTCTGTTTTTGCTCTGTTTTAGGAAGAATCAGCTGAAGAAAGTCTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGTTTGAGCTCGATTTCAATTCTGAAATTAGGGTTTTTTAGGGTTTTGATTTTGGGGTTGGAACTAATTTGGTTTTTTTTTTTAAAAAAAACAGGGGATATTACAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTCGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCGACTCACCGGAACTCCCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCTACGCCCACCGCTTCCGGATCTGAAAAACCCGACCCGAAGCAGCCGGTTTGCTCGTCGGAAGAACAGAGTACGATCACAGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGGGTCTCCGATTTGATCGTCAACGACGATTTCTATGTGGGTTTTGAAGAACTCGACAGCCCAATGACCGACGACTGCTTTTCTGATCAGTTTCCGGCGAATTTCGACCTTCCATGGTTGTTTAACGGAAACCCAGACAGCGAAATTGTAAAACTCCCCTGTTAA

mRNA sequence

AAAAAAAAAAAAAAAATAGAAAGAAAAAGAAGCTGATCAAGAAGCTGAGACAAAGGTACAGATTCTCAGAGCAGAGTTCTGTCTTTGATAATGGAAGTCGATTGGGATCTTCACGCCGTCGTCAGAGGCTACTCCGCCGCGCCCTCCGCTGCCACCATTATTCCTTCTTCTTCTTCTTCTGTTTCTTCGTCTTCCAATAACTCTATCCCTTTTTCTTTTGGTAGAGACCTAACCAACCAAACAAAAAACCATTTCTTTTCCCTTCAAGATCCATTTCAACCCCCTAATTGTAATTCCACTGAGGAATTGCATGAGCTTTTCAAACCCTTTTTCCCCAAATCTCAACCCTCTCCTCCGCCGCTGCCTCCATCGCCTGCTCTGCCGCTTCTTTCTTCTCCGGCTGCTAAAATTCTGACCCATCAGAAGCAACAACAGAGCACCCATCTCCCCAAACAGCTCCATTCAGCCCCTGTTTCTGCTCCGAGATCCAAACGCCGGAAGAATCAGCTGAAGAAAGTCTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGGGATATTACAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTCGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCGACTCACCGGAACTCCCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCTACGCCCACCGCTTCCGGATCTGAAAAACCCGACCCGAAGCAGCCGGTTTGCTCGTCGGAAGAACAGAGTACGATCACAGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGGGTCTCCGATTTGATCGTCAACGACGATTTCTATGTGGGTTTTGAAGAACTCGACAGCCCAATGACCGACGACTGCTTTTCTGATCAGTTTCCGGCGAATTTCGACCTTCCATGGTTGTTTAACGGAAACCCAGACAGCGAAATTGTAAAACTCCCCTGTTAA

Coding sequence (CDS)

ATGGAAGTCGATTGGGATCTTCACGCCGTCGTCAGAGGCTACTCCGCCGCGCCCTCCGCTGCCACCATTATTCCTTCTTCTTCTTCTTCTGTTTCTTCGTCTTCCAATAACTCTATCCCTTTTTCTTTTGGTAGAGACCTAACCAACCAAACAAAAAACCATTTCTTTTCCCTTCAAGATCCATTTCAACCCCCTAATTGTAATTCCACTGAGGAATTGCATGAGCTTTTCAAACCCTTTTTCCCCAAATCTCAACCCTCTCCTCCGCCGCTGCCTCCATCGCCTGCTCTGCCGCTTCTTTCTTCTCCGGCTGCTAAAATTCTGACCCATCAGAAGCAACAACAGAGCACCCATCTCCCCAAACAGCTCCATTCAGCCCCTGTTTCTGCTCCGAGATCCAAACGCCGGAAGAATCAGCTGAAGAAAGTCTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGGGATATTACAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTCGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCGACTCACCGGAACTCCCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCTACGCCCACCGCTTCCGGATCTGAAAAACCCGACCCGAAGCAGCCGGTTTGCTCGTCGGAAGAACAGAGTACGATCACAGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGGGTCTCCGATTTGATCGTCAACGACGATTTCTATGTGGGTTTTGAAGAACTCGACAGCCCAATGACCGACGACTGCTTTTCTGATCAGTTTCCGGCGAATTTCGACCTTCCATGGTTGTTTAACGGAAACCCAGACAGCGAAATTGTAAAACTCCCCTGTTAA

Protein sequence

MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQDPFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEELDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC
Homology
BLAST of Lsi05G020690 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.0e-55
Identity = 154/325 (47.38%), Postives = 188/325 (57.85%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQD 60
           M  DWDLHAVVRG SA  S+AT   ++  S   SS+ +  F+ GR     +      L  
Sbjct: 1   MADDWDLHAVVRGCSAVSSSAT---TTVYSPGVSSHTNPIFTVGRQSNAVSFGEIRDLYT 60

Query: 61  PFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQSTHLP 120
           PF       T+E   +   F   + P  P  P +   PL  S ++  +T +    +T   
Sbjct: 61  PF-------TQE--SVVSSFSCINYPEEPRKPQNQKRPLSLSASSGSVTSKPSGSNT--- 120

Query: 121 KQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 180
                      RSKRRK Q KKVC V AE+L+SD+WAWRKYGQKPIKGSPYPRGYYRCS+
Sbjct: 121 ----------SRSKRRKIQHKKVCHVAAEALNSDVWAWRKYGQKPIKGSPYPRGYYRCST 180

Query: 181 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPI------TPTPTA 240
           SKGC+ARKQVERNRSDP MFIVTYTAEHNHPAPTHRNSLAGSTRQKP       +PT T 
Sbjct: 181 SKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSDQQTSKSPTTTI 240

Query: 241 SGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEEL 300
           +         PV S++E     E      +L    D E+D L +SD +V+DDF+ G EE 
Sbjct: 241 ATYS----SSPVTSADEFVLPVEDHLAVGDL----DGEEDLLSLSDTVVSDDFFDGLEEF 289

Query: 301 DSPMTDDCFS-DQFPANFDLPWLFN 319
                 D FS +  PA+FDL W+ N
Sbjct: 301 ---AAGDSFSGNSAPASFDLSWVVN 289

BLAST of Lsi05G020690 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-43
Identity = 140/338 (41.42%), Postives = 175/338 (51.78%), Query Frame = 0

Query: 4   DWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQDPFQ 63
           DWDL AVVR             S SSSVS++++ +       D+ N  +      QDP  
Sbjct: 5   DWDLFAVVR-------------SCSSSVSTTNSCA---GHEDDIGNCKQQ-----QDPPP 64

Query: 64  PP---NCNSTEELHELFKPFFPKSQP-----SPPPLPPSPALPLLSSPAAKILTHQKQ-- 123
           PP     +S  EL +  KPF P +       SPPPL P P     SSP+  IL  Q+Q  
Sbjct: 65  PPLFQASSSCNELQDSCKPFLPVTTTTTTTWSPPPLLPPPK---ASSPSPNILLKQEQVL 124

Query: 124 --QQSTHLPKQLHSAPVSAP----------------------RSKRRKNQLKK-VCQVPA 183
              Q    P  +   P S                        RS++RKNQ K+ +C V  
Sbjct: 125 LESQDQKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQ 184

Query: 184 ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEH 243
           E+LSSD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC+ARKQVER+  DP +FIVTYT EH
Sbjct: 185 ENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEH 244

Query: 244 NHPAPTHRNSLAGSTRQK--PITPTPTASGSEKPD---------PKQPVCSSEE------ 284
            HP PTHRNSLAGSTR K  P+ P P    S   D         P  P+  +++      
Sbjct: 245 THPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNG 304

BLAST of Lsi05G020690 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-34
Identity = 82/159 (51.57%), Postives = 103/159 (64.78%), Query Frame = 0

Query: 101 SSPAAKILTHQKQQQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPA----------ES 160
           +SP   +L       S+    Q+ S+P +    KRRK+Q KKV  +PA          E 
Sbjct: 154 NSPRNCLLVDNNNNTSSCSQVQISSSPRNL-GIKRRKSQAKKVVCIPAPAAMNSRSSGEV 213

Query: 161 LSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNH 220
           + SD+WAWRKYGQKPIKGSPYPRGYYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNH
Sbjct: 214 VPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNH 273

Query: 221 PAPTHRNSLAGSTRQ------KPITPTPTASGSEKPDPK 244
           P PT RN+LAGSTR        P + + TA+ +  P  +
Sbjct: 274 PWPTQRNALAGSTRSSSSSSLNPSSKSSTAAATTSPSSR 311

BLAST of Lsi05G020690 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 1.0e-33
Identity = 87/182 (47.80%), Postives = 114/182 (62.64%), Query Frame = 0

Query: 128 VSAPRS---KRRKNQLKKVCQVPA----------ESLSSDIWAWRKYGQKPIKGSPYPRG 187
           +S+PR+   KRRK+Q KKV  +PA          E + SD+WAWRKYGQKPIKGSP+PRG
Sbjct: 179 ISSPRNLGLKRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPFPRG 238

Query: 188 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTA 247
           YYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNHP P  RN+LAGSTR        + 
Sbjct: 239 YYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRS-------ST 298

Query: 248 SGSEKPDPKQPVCSSEEQSTI-------TESKEEKEELLMA---EDEEDDDLGVSDLIVN 287
           S S  P+P +P  ++   S+I         S       L +   +DE  DD+ + ++  +
Sbjct: 299 SSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMELENVDDD 353

BLAST of Lsi05G020690 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-33
Identity = 113/270 (41.85%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 6   DLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQDPFQPP 65
           DL A+VRGYS +  A     S  SS + S +  +P                S  +P    
Sbjct: 5   DLEAIVRGYSGSGDAF----SGESSGTFSPSFCLPMETS------------SFYEP--EM 64

Query: 66  NCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQS-----THLP 125
             +  +EL EL+KPF+P S  +   L  S +LP  S P        K+Q+S     ++  
Sbjct: 65  ETSGLDELGELYKPFYPFS--TQTILTSSVSLPEDSKP----FRDDKKQRSHGCLLSNGS 124

Query: 126 KQLHSAPVSAPRSKRRKNQLKKVC-QVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCS 185
           +  H     +   K +KNQ K+V  QV  E+L SD WAWRKYGQKPIKGSPYPR YYRCS
Sbjct: 125 RADHIRISESKSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCS 184

Query: 186 SSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSEK 245
           SSKGC+ARKQVERN  +P  F +TYT EHNH  PT RNSLAGSTR K   P PT +   +
Sbjct: 185 SSKGCLARKQVERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTLTKKSE 244

Query: 246 PDPKQPVCSSEEQSTITESKEEKEELLMAE 270
            +      S+    +  ES    +E+ +AE
Sbjct: 245 KEVVSSPTSNPMIPSADESSVAVQEMSVAE 250

BLAST of Lsi05G020690 vs. ExPASy TrEMBL
Match: A0A5D3D3F9 (WRKY transcription factor 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004970 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 307/337 (91.10%), Postives = 312/337 (92.58%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLT-NQTKNHFFSLQ 60
           MEVDWDLHAVVRGYSAAPSAATI+PSSSSS    SNNS+PFSFGRDLT NQTKNHFFSLQ
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSSF---SNNSVPFSFGRDLTNNQTKNHFFSLQ 60

Query: 61  DPFQPPNCNSTEELHELFKPFFPKSQPS------PPPLPPSPALPLLSSPAAKILTHQKQ 120
           DPFQP NCNST+ELHELFKPFFPKSQPS      PPP PP  A PLLSSPA KILTH K 
Sbjct: 61  DPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSPAPKILTHHK- 120

Query: 121 QQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180
            QSTHLPKQLHS  VSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR
Sbjct: 121 -QSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180

Query: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPT 240
           GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TPT T
Sbjct: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPTTT 240

Query: 241 ASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300
           ASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE
Sbjct: 241 ASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300

Query: 301 LDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           LDSP+TDDCFSDQFPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 LDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 332

BLAST of Lsi05G020690 vs. ExPASy TrEMBL
Match: A0A1S3AVV5 (WRKY transcription factor 22 OS=Cucumis melo OX=3656 GN=LOC103483243 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.4e-165
Identity = 306/337 (90.80%), Postives = 311/337 (92.28%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLT-NQTKNHFFSLQ 60
           MEVDWDLHAVVRGYSAAPSAATI+PSS    SS SNNS+PFSFGRDLT NQTKNHFFSLQ
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSS----SSFSNNSVPFSFGRDLTNNQTKNHFFSLQ 60

Query: 61  DPFQPPNCNSTEELHELFKPFFPKSQPS------PPPLPPSPALPLLSSPAAKILTHQKQ 120
           DPFQP NCNST+ELHELFKPFFPKSQPS      PPP PP  A PLLSSPA KILTH K 
Sbjct: 61  DPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSPAPKILTHHK- 120

Query: 121 QQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180
            QSTHLPKQLHS  VSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR
Sbjct: 121 -QSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180

Query: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPT 240
           GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TPT T
Sbjct: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPTTT 240

Query: 241 ASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300
           ASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE
Sbjct: 241 ASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300

Query: 301 LDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           LDSP+TDDCFSDQFPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 LDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 331

BLAST of Lsi05G020690 vs. ExPASy TrEMBL
Match: A0A0A0L7C1 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121580 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 302/337 (89.61%), Postives = 311/337 (92.28%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNS--IPFSFGRDLT-NQTKNHFFS 60
           MEVDWDLHAVVRGYSAAPSAATI+P+SSSS SSSS+N+  +PFSFGRDLT NQ KNHFFS
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFS 60

Query: 61  LQDPFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALP----LLSSPAAKILTHQKQ 120
           LQDPFQP NCNST+ELHELFKPFFPKSQPSP P PP PA P    L S PA KILTHQK 
Sbjct: 61  LQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK- 120

Query: 121 QQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180
            QSTHLPKQLHS   SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR
Sbjct: 121 -QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180

Query: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPT 240
           GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITP+ T
Sbjct: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTT 240

Query: 241 ASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300
           ASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGFEE
Sbjct: 241 ASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEE 300

Query: 301 LDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           LDSP+TDDCFSD FPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 LDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 335

BLAST of Lsi05G020690 vs. ExPASy TrEMBL
Match: E7CEW7 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY18 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.7e-162
Identity = 304/338 (89.94%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIP--SSSSSVSSSSNNS-IPFSFGRDLT-NQTKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATI+P  SSSSS SSSSNN  +PFSFGRDLT NQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALP----LLSSPAAKILTHQK 120
           SLQDPFQP NCNST+ELHELFKPFFPKSQPSP P PP PA P    L S PA KILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QQQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
             QSTHLPKQLHS   SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 121 --QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTP 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITP+ 
Sbjct: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240

Query: 241 TASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFE 300
           TASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGFE
Sbjct: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300

Query: 301 ELDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           ELDSP+TDDCFSD FPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336

BLAST of Lsi05G020690 vs. ExPASy TrEMBL
Match: A0A6J1EEC7 (WRKY transcription factor 22 OS=Cucurbita moschata OX=3662 GN=LOC111431800 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.3e-156
Identity = 292/329 (88.75%), Postives = 305/329 (92.71%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQD 60
           MEVDWDLHAVVRGYSA  SAATI  S   SVSS+SNNS PF+FGRDLTNQT NHFFSLQD
Sbjct: 3   MEVDWDLHAVVRGYSAVSSAATIPSSDFYSVSSTSNNSTPFAFGRDLTNQT-NHFFSLQD 62

Query: 61  PFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQSTHLP 120
           PF+ PNCNSTEELHELFKPF PK  PSPPP PPS  L LLSSPAAKILTHQKQ+Q+TH+P
Sbjct: 63  PFEGPNCNSTEELHELFKPFSPKPLPSPPPPPPSAPL-LLSSPAAKILTHQKQRQNTHVP 122

Query: 121 KQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 180
           KQLHSAPVSA RSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 123 KQLHSAPVSASRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 182

Query: 181 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSEKP 240
           SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP T TP +SGSEKP
Sbjct: 183 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPNTQTPASSGSEKP 242

Query: 241 DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEELDSPMTD 300
           D KQPVCSSE+QSTITESKEEKEELL AEDEEDDDLGVSDLIVNDDFY+GFEELDSP+TD
Sbjct: 243 DSKQPVCSSEDQSTITESKEEKEELL-AEDEEDDDLGVSDLIVNDDFYMGFEELDSPITD 302

Query: 301 DCFSDQFPANFDLPWLFNGNPDSEIVKLP 330
           +CFSDQFPANF+LPW FNG+PDSEIVKLP
Sbjct: 303 ECFSDQFPANFELPWSFNGDPDSEIVKLP 328

BLAST of Lsi05G020690 vs. NCBI nr
Match: XP_038880705.1 (WRKY transcription factor 22 [Benincasa hispida])

HSP 1 Score: 613.2 bits (1580), Expect = 1.3e-171
Identity = 312/330 (94.55%), Postives = 314/330 (95.15%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQD 60
           MEVDWDLHAVVRGYSA  S ATIIPSSS   SSSSNNSIPFSF RDLTNQTKNHFFSLQD
Sbjct: 1   MEVDWDLHAVVRGYSAVSSTATIIPSSS---SSSSNNSIPFSFSRDLTNQTKNHFFSLQD 60

Query: 61  PFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQSTHLP 120
           PFQPPNCNST+ELHELFKPFFPKSQPSPPP PP PA PLLSSPAAKILT  KQQQSTHLP
Sbjct: 61  PFQPPNCNSTQELHELFKPFFPKSQPSPPPPPPPPAPPLLSSPAAKILTPPKQQQSTHLP 120

Query: 121 KQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 180
           KQLHS  VSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 121 KQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 180

Query: 181 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSEKP 240
           SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGS KP
Sbjct: 181 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSGKP 240

Query: 241 DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEELDSPMTD 300
           DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEELDSP+TD
Sbjct: 241 DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEELDSPITD 300

Query: 301 DCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           DCFSD FPANFDLPWLFNGNPDSEIVKLPC
Sbjct: 301 DCFSDPFPANFDLPWLFNGNPDSEIVKLPC 327

BLAST of Lsi05G020690 vs. NCBI nr
Match: TYK17626.1 (WRKY transcription factor 22 [Cucumis melo var. makuwa])

HSP 1 Score: 592.4 bits (1526), Expect = 2.4e-165
Identity = 307/337 (91.10%), Postives = 312/337 (92.58%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLT-NQTKNHFFSLQ 60
           MEVDWDLHAVVRGYSAAPSAATI+PSSSSS    SNNS+PFSFGRDLT NQTKNHFFSLQ
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSSF---SNNSVPFSFGRDLTNNQTKNHFFSLQ 60

Query: 61  DPFQPPNCNSTEELHELFKPFFPKSQPS------PPPLPPSPALPLLSSPAAKILTHQKQ 120
           DPFQP NCNST+ELHELFKPFFPKSQPS      PPP PP  A PLLSSPA KILTH K 
Sbjct: 61  DPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSPAPKILTHHK- 120

Query: 121 QQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180
            QSTHLPKQLHS  VSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR
Sbjct: 121 -QSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180

Query: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPT 240
           GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TPT T
Sbjct: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPTTT 240

Query: 241 ASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300
           ASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE
Sbjct: 241 ASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300

Query: 301 LDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           LDSP+TDDCFSDQFPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 LDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 332

BLAST of Lsi05G020690 vs. NCBI nr
Match: XP_008437986.1 (PREDICTED: WRKY transcription factor 22 [Cucumis melo])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 306/337 (90.80%), Postives = 311/337 (92.28%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLT-NQTKNHFFSLQ 60
           MEVDWDLHAVVRGYSAAPSAATI+PSS    SS SNNS+PFSFGRDLT NQTKNHFFSLQ
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSS----SSFSNNSVPFSFGRDLTNNQTKNHFFSLQ 60

Query: 61  DPFQPPNCNSTEELHELFKPFFPKSQPS------PPPLPPSPALPLLSSPAAKILTHQKQ 120
           DPFQP NCNST+ELHELFKPFFPKSQPS      PPP PP  A PLLSSPA KILTH K 
Sbjct: 61  DPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSPAPKILTHHK- 120

Query: 121 QQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180
            QSTHLPKQLHS  VSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR
Sbjct: 121 -QSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPR 180

Query: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPT 240
           GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TPT T
Sbjct: 181 GYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPTTT 240

Query: 241 ASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300
           ASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE
Sbjct: 241 ASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEE 300

Query: 301 LDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           LDSP+TDDCFSDQFPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 LDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 331

BLAST of Lsi05G020690 vs. NCBI nr
Match: NP_001267594.1 (WRKY transcription factor 22-like [Cucumis sativus] >ADU52510.1 WRKY protein [Cucumis sativus] >KAE8650243.1 hypothetical protein Csa_011009 [Cucumis sativus])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 304/338 (89.94%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIP--SSSSSVSSSSNNS-IPFSFGRDLT-NQTKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATI+P  SSSSS SSSSNN  +PFSFGRDLT NQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALP----LLSSPAAKILTHQK 120
           SLQDPFQP NCNST+ELHELFKPFFPKSQPSP P PP PA P    L S PA KILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QQQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
             QSTHLPKQLHS   SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 121 --QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTP 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITP+ 
Sbjct: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240

Query: 241 TASGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFE 300
           TASGSEK DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGFE
Sbjct: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300

Query: 301 ELDSPMTDDCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           ELDSP+TDDCFSD FPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336

BLAST of Lsi05G020690 vs. NCBI nr
Match: KAG6582635.1 (Proline iminopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564.7 bits (1454), Expect = 5.3e-157
Identity = 292/330 (88.48%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQD 60
           MEVDWDLHAVVRGYSA  SAATI  S   SVSS+SNNS PF+FGRDLTNQT NHFFSLQD
Sbjct: 3   MEVDWDLHAVVRGYSAVSSAATIPSSDFYSVSSTSNNSTPFAFGRDLTNQT-NHFFSLQD 62

Query: 61  PFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQSTHLP 120
           PF+ PNCNSTEELHELFKPF PK  PSPPP PPS  L LLSSPAAKILTHQKQ+Q+TH+P
Sbjct: 63  PFEGPNCNSTEELHELFKPFSPKPLPSPPPPPPSAPL-LLSSPAAKILTHQKQRQNTHVP 122

Query: 121 KQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 180
           KQLHS PVSA RSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 123 KQLHSVPVSASRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 182

Query: 181 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSEKP 240
           SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP T TP +SGSEKP
Sbjct: 183 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPNTQTPASSGSEKP 242

Query: 241 DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEELDSPMTD 300
           D KQPVCSSE+QSTITESKEEKEELL AEDEEDDDLGVSDLIVNDDFY+GFEELDSP+TD
Sbjct: 243 DSKQPVCSSEDQSTITESKEEKEELL-AEDEEDDDLGVSDLIVNDDFYMGFEELDSPITD 302

Query: 301 DCFSDQFPANFDLPWLFNGNPDSEIVKLPC 331
           +CFSDQFPANF+LPW FNG+PDSEIVKLPC
Sbjct: 303 ECFSDQFPANFELPWSFNGDPDSEIVKLPC 329

BLAST of Lsi05G020690 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 216.1 bits (549), Expect = 4.3e-56
Identity = 154/325 (47.38%), Postives = 188/325 (57.85%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQD 60
           M  DWDLHAVVRG SA  S+AT   ++  S   SS+ +  F+ GR     +      L  
Sbjct: 1   MADDWDLHAVVRGCSAVSSSAT---TTVYSPGVSSHTNPIFTVGRQSNAVSFGEIRDLYT 60

Query: 61  PFQPPNCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQSTHLP 120
           PF       T+E   +   F   + P  P  P +   PL  S ++  +T +    +T   
Sbjct: 61  PF-------TQE--SVVSSFSCINYPEEPRKPQNQKRPLSLSASSGSVTSKPSGSNT--- 120

Query: 121 KQLHSAPVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSS 180
                      RSKRRK Q KKVC V AE+L+SD+WAWRKYGQKPIKGSPYPRGYYRCS+
Sbjct: 121 ----------SRSKRRKIQHKKVCHVAAEALNSDVWAWRKYGQKPIKGSPYPRGYYRCST 180

Query: 181 SKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPI------TPTPTA 240
           SKGC+ARKQVERNRSDP MFIVTYTAEHNHPAPTHRNSLAGSTRQKP       +PT T 
Sbjct: 181 SKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSDQQTSKSPTTTI 240

Query: 241 SGSEKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFEEL 300
           +         PV S++E     E      +L    D E+D L +SD +V+DDF+ G EE 
Sbjct: 241 ATYS----SSPVTSADEFVLPVEDHLAVGDL----DGEEDLLSLSDTVVSDDFFDGLEEF 289

Query: 301 DSPMTDDCFS-DQFPANFDLPWLFN 319
                 D FS +  PA+FDL W+ N
Sbjct: 301 ---AAGDSFSGNSAPASFDLSWVVN 289

BLAST of Lsi05G020690 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 178.3 bits (451), Expect = 9.9e-45
Identity = 140/338 (41.42%), Postives = 175/338 (51.78%), Query Frame = 0

Query: 4   DWDLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQDPFQ 63
           DWDL AVVR             S SSSVS++++ +       D+ N  +      QDP  
Sbjct: 5   DWDLFAVVR-------------SCSSSVSTTNSCA---GHEDDIGNCKQQ-----QDPPP 64

Query: 64  PP---NCNSTEELHELFKPFFPKSQP-----SPPPLPPSPALPLLSSPAAKILTHQKQ-- 123
           PP     +S  EL +  KPF P +       SPPPL P P     SSP+  IL  Q+Q  
Sbjct: 65  PPLFQASSSCNELQDSCKPFLPVTTTTTTTWSPPPLLPPPK---ASSPSPNILLKQEQVL 124

Query: 124 --QQSTHLPKQLHSAPVSAP----------------------RSKRRKNQLKK-VCQVPA 183
              Q    P  +   P S                        RS++RKNQ K+ +C V  
Sbjct: 125 LESQDQKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQ 184

Query: 184 ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEH 243
           E+LSSD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC+ARKQVER+  DP +FIVTYT EH
Sbjct: 185 ENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEH 244

Query: 244 NHPAPTHRNSLAGSTRQK--PITPTPTASGSEKPD---------PKQPVCSSEE------ 284
            HP PTHRNSLAGSTR K  P+ P P    S   D         P  P+  +++      
Sbjct: 245 THPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNG 304

BLAST of Lsi05G020690 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-35
Identity = 82/159 (51.57%), Postives = 103/159 (64.78%), Query Frame = 0

Query: 101 SSPAAKILTHQKQQQSTHLPKQLHSAPVSAPRSKRRKNQLKKVCQVPA----------ES 160
           +SP   +L       S+    Q+ S+P +    KRRK+Q KKV  +PA          E 
Sbjct: 154 NSPRNCLLVDNNNNTSSCSQVQISSSPRNL-GIKRRKSQAKKVVCIPAPAAMNSRSSGEV 213

Query: 161 LSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNH 220
           + SD+WAWRKYGQKPIKGSPYPRGYYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNH
Sbjct: 214 VPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNH 273

Query: 221 PAPTHRNSLAGSTRQ------KPITPTPTASGSEKPDPK 244
           P PT RN+LAGSTR        P + + TA+ +  P  +
Sbjct: 274 PWPTQRNALAGSTRSSSSSSLNPSSKSSTAAATTSPSSR 311

BLAST of Lsi05G020690 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 145.6 bits (366), Expect = 7.1e-35
Identity = 87/182 (47.80%), Postives = 114/182 (62.64%), Query Frame = 0

Query: 128 VSAPRS---KRRKNQLKKVCQVPA----------ESLSSDIWAWRKYGQKPIKGSPYPRG 187
           +S+PR+   KRRK+Q KKV  +PA          E + SD+WAWRKYGQKPIKGSP+PRG
Sbjct: 179 ISSPRNLGLKRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPFPRG 238

Query: 188 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTA 247
           YYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNHP P  RN+LAGSTR        + 
Sbjct: 239 YYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRS-------ST 298

Query: 248 SGSEKPDPKQPVCSSEEQSTI-------TESKEEKEELLMA---EDEEDDDLGVSDLIVN 287
           S S  P+P +P  ++   S+I         S       L +   +DE  DD+ + ++  +
Sbjct: 299 SSSSNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMELENVDDD 353

BLAST of Lsi05G020690 vs. TAIR 10
Match: AT4G23550.1 (WRKY family transcription factor )

HSP 1 Score: 145.2 bits (365), Expect = 9.3e-35
Identity = 113/270 (41.85%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 6   DLHAVVRGYSAAPSAATIIPSSSSSVSSSSNNSIPFSFGRDLTNQTKNHFFSLQDPFQPP 65
           DL A+VRGYS +  A     S  SS + S +  +P                S  +P    
Sbjct: 5   DLEAIVRGYSGSGDAF----SGESSGTFSPSFCLPMETS------------SFYEP--EM 64

Query: 66  NCNSTEELHELFKPFFPKSQPSPPPLPPSPALPLLSSPAAKILTHQKQQQS-----THLP 125
             +  +EL EL+KPF+P S  +   L  S +LP  S P        K+Q+S     ++  
Sbjct: 65  ETSGLDELGELYKPFYPFS--TQTILTSSVSLPEDSKP----FRDDKKQRSHGCLLSNGS 124

Query: 126 KQLHSAPVSAPRSKRRKNQLKKVC-QVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCS 185
           +  H     +   K +KNQ K+V  QV  E+L SD WAWRKYGQKPIKGSPYPR YYRCS
Sbjct: 125 RADHIRISESKSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCS 184

Query: 186 SSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTPTASGSEK 245
           SSKGC+ARKQVERN  +P  F +TYT EHNH  PT RNSLAGSTR K   P PT +   +
Sbjct: 185 SSKGCLARKQVERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTLTKKSE 244

Query: 246 PDPKQPVCSSEEQSTITESKEEKEELLMAE 270
            +      S+    +  ES    +E+ +AE
Sbjct: 245 KEVVSSPTSNPMIPSADESSVAVQEMSVAE 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O046096.0e-5547.38WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Q9FLX81.4e-4341.42Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
O647472.0e-3451.57Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9SA801.0e-3347.80Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
Q9SUS11.3e-3341.85Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ... [more]
Match NameE-valueIdentityDescription
A0A5D3D3F91.1e-16591.10WRKY transcription factor 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AVV57.4e-16590.80WRKY transcription factor 22 OS=Cucumis melo OX=3656 GN=LOC103483243 PE=4 SV=1[more]
A0A0A0L7C11.5e-16289.61WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121580 PE=4 S... [more]
E7CEW77.7e-16289.94WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY18 PE=2 SV=1[more]
A0A6J1EEC71.3e-15688.75WRKY transcription factor 22 OS=Cucurbita moschata OX=3662 GN=LOC111431800 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_038880705.11.3e-17194.55WRKY transcription factor 22 [Benincasa hispida][more]
TYK17626.12.4e-16591.10WRKY transcription factor 22 [Cucumis melo var. makuwa][more]
XP_008437986.11.5e-16490.80PREDICTED: WRKY transcription factor 22 [Cucumis melo][more]
NP_001267594.11.6e-16189.94WRKY transcription factor 22-like [Cucumis sativus] >ADU52510.1 WRKY protein [Cu... [more]
KAG6582635.15.3e-15788.48Proline iminopeptidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G01250.14.3e-5647.38WRKY family transcription factor [more]
AT5G52830.19.9e-4541.42WRKY DNA-binding protein 27 [more]
AT2G34830.11.4e-3551.57WRKY DNA-binding protein 35 [more]
AT1G30650.17.1e-3547.80WRKY DNA-binding protein 14 [more]
AT4G23550.19.3e-3541.85WRKY family transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 152..212
e-value: 7.6E-37
score: 138.4
IPR003657WRKY domainPFAMPF03106WRKYcoord: 154..211
e-value: 4.3E-25
score: 87.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 147..213
score: 34.77314
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 136..206
e-value: 1.9E-31
score: 109.5
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 150..213
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 79..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..238
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..275
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..99
NoneNo IPR availablePANTHERPTHR32096:SF92TRANSCRIPTION FACTOR WRKY FAMILY-RELATEDcoord: 1..311
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 1..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G020690.1Lsi05G020690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding