Lsi05G020240 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G020240 vs. ExPASy TrEMBL
Match: A0A0A0L7H7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122560 PE=4 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 8.8e-291
Identity = 535/773 (69.21%), Postives = 563/773 (72.83%), Query Frame = 0

Query: 4    VLMERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVL 63
            VLME  G+ DIHDCTIKLRVNPQK+RDKV IGCGAGFGGDRPTAALKLLQRVKNLNYLVL
Sbjct: 583  VLMEGHGQADIHDCTIKLRVNPQKQRDKVCIGCGAGFGGDRPTAALKLLQRVKNLNYLVL 642

Query: 64   ECLAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNV 123
            ECLAERTLAD YQVMLSGGDGYD R  +WMKLLLPLAMKRNICIITNMGAMDPP AQ+NV
Sbjct: 643  ECLAERTLADHYQVMLSGGDGYDPRIADWMKLLLPLAMKRNICIITNMGAMDPPAAQQNV 702

Query: 124  IEIAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLA 183
            IE+AGSLGLNVSVAVAYE SVKE GISTY+G APIVECLEKYHPNVIITSRVADAALFLA
Sbjct: 703  IEVAGSLGLNVSVAVAYEGSVKESGISTYMGGAPIVECLEKYHPNVIITSRVADAALFLA 762

Query: 184  PMVGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYR 243
            PM                                                          
Sbjct: 763  PM---------------------------------------------------------- 822

Query: 244  KYIQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHT 303
                           VYELGWNWDD P LAQGILAGHLLECGCQLTGGYFMHPG      
Sbjct: 823  ---------------VYELGWNWDDFPLLAQGILAGHLLECGCQLTGGYFMHPG------ 882

Query: 304  HPNPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCA 363
                           DKYRSMS QQLLNISLPYAEVECDGK TVAK EE+GGLLNFSTCA
Sbjct: 883  ---------------DKYRSMSFQQLLNISLPYAEVECDGKLTVAKPEESGGLLNFSTCA 942

Query: 364  EQLLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCG 423
            EQLLYE+G+PSAYITPD+++                               L +  +DCG
Sbjct: 943  EQLLYEIGNPSAYITPDLVVDFSNVSFCSISSSRVLCSGAKPSIQGVPEKLLQLAPKDCG 1002

Query: 424  WKGWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSV 483
            WKGWGEISYGGRECVLRAKAAEYLVRSWMEE LIGIN+HIVSY IGLDSLKASSN SN V
Sbjct: 1003 WKGWGEISYGGRECVLRAKAAEYLVRSWMEELLIGINRHIVSYTIGLDSLKASSNGSNCV 1062

Query: 484  EDIRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLIC 543
            EDIRLRMDGLF+QKEHALLFV+EFTALYTNGPAGGGGIS                     
Sbjct: 1063 EDIRLRMDGLFEQKEHALLFVKEFTALYTNGPAGGGGIS--------------------- 1122

Query: 544  YLIESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDS 603
                  TGYKKEIVLEKQL                   VGRENIFWQT V CT AVKLDS
Sbjct: 1123 ------TGYKKEIVLEKQL-------------------VGRENIFWQTEVTCTEAVKLDS 1182

Query: 604  QPTDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSR 663
            Q TDL+KDPAE CSSPRVTLPCPIS +A++ CTGS PPE GHSPIPSGQEIALYNVAHSR
Sbjct: 1183 QSTDLQKDPAEACSSPRVTLPCPISDHADELCTGSLPPEMGHSPIPSGQEIALYNVAHSR 1215

Query: 664  AGDKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDE 723
            AGDKGNDLNFS+IPH PSDIERL+MIITPEWVMRVLSVLHNST F SS+AD+KR++WV E
Sbjct: 1243 AGDKGNDLNFSLIPHCPSDIERLKMIITPEWVMRVLSVLHNSTRFHSSNADEKRNEWVSE 1215

Query: 724  HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
             VKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDLILNQ IVLPP
Sbjct: 1303 DVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLILNQLIVLPP 1215

BLAST of Lsi05G020240 vs. ExPASy TrEMBL
Match: A0A1S3AV50 (uncharacterized protein LOC103483286 OS=Cucumis melo OX=3656 GN=LOC103483286 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.7e-289
Identity = 531/771 (68.87%), Postives = 562/771 (72.89%), Query Frame = 0

Query: 6   MERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 65
           MER  + DIHDCTIKLRVNP+K+RDKV IGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC
Sbjct: 1   MERHSQADIHDCTIKLRVNPKKQRDKVCIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 60

Query: 66  LAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIE 125
           LAERTLAD YQVMLSGGDGYDSR   WMKLLLPL+MKRNICIITNMGAMDP  AQ+ VIE
Sbjct: 61  LAERTLADHYQVMLSGGDGYDSRIAEWMKLLLPLSMKRNICIITNMGAMDPLAAQQKVIE 120

Query: 126 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAPM 185
           +AGSLGLNVSVAVAYE SVKE GISTY+G APIVECLEKYHPNVIITSRVADAALFLAPM
Sbjct: 121 VAGSLGLNVSVAVAYEGSVKESGISTYMGGAPIVECLEKYHPNVIITSRVADAALFLAPM 180

Query: 186 VGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRKY 245
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 246 IQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTHP 305
                        VYELGWNWDD P LAQGILAGHLLECGCQLTGGYFMHPG        
Sbjct: 241 -------------VYELGWNWDDFPLLAQGILAGHLLECGCQLTGGYFMHPG-------- 300

Query: 306 NPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAEQ 365
                        DKYRSMS QQLLNISLPYAEVECDGK TVAK EE+GGLLNFSTCAEQ
Sbjct: 301 -------------DKYRSMSFQQLLNISLPYAEVECDGKLTVAKPEESGGLLNFSTCAEQ 360

Query: 366 LLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGWK 425
           LLYE+GDPSAYITPD+++                               L +  +DCGWK
Sbjct: 361 LLYEIGDPSAYITPDLVVDFSNVSFCSISSSRVVCSGAKPSIQGVPEKLLQLAPKDCGWK 420

Query: 426 GWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVED 485
           GWGEISYGGRECVLRAKAAEYLVRSWMEE LIGIN+HIVSY IGLDSLKASSNSSN +ED
Sbjct: 421 GWGEISYGGRECVLRAKAAEYLVRSWMEELLIGINEHIVSYTIGLDSLKASSNSSNCIED 480

Query: 486 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICYL 545
           IRLRMDGLF+QKEHALLFV+EFTALYTNGPAGGGGIS                       
Sbjct: 481 IRLRMDGLFEQKEHALLFVKEFTALYTNGPAGGGGIS----------------------- 540

Query: 546 IESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQP 605
               TGYKKEIVLEKQL                   VGRENIFWQT VKC+ AVKLDSQ 
Sbjct: 541 ----TGYKKEIVLEKQL-------------------VGRENIFWQTEVKCSEAVKLDSQS 600

Query: 606 TDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRAG 665
           TDL+KDPAE CSSPRVTLPCPIS++AEK CTGSFPPETGHSPIPSGQEIALY+VAHSRAG
Sbjct: 601 TDLQKDPAEACSSPRVTLPCPISSHAEKLCTGSFPPETGHSPIPSGQEIALYDVAHSRAG 631

Query: 666 DKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEHV 725
           DKGNDLNFS+IPHYPSDIERL+MIITPEWVMRVLS LHN T F SS+A +KR++WV+E V
Sbjct: 661 DKGNDLNFSLIPHYPSDIERLKMIITPEWVMRVLSGLHNLTRFHSSNAGEKRNEWVNEDV 631

Query: 726 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           KVEIYEVK IHSLNVVVRNILDGGVNCSRRIDRHGKT+SDLILNQ IVLPP
Sbjct: 721 KVEIYEVKSIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLILNQLIVLPP 631

BLAST of Lsi05G020240 vs. ExPASy TrEMBL
Match: A0A6J1IE50 (uncharacterized protein LOC111474742 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111474742 PE=4 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 4.1e-288
Identity = 521/771 (67.57%), Postives = 564/771 (73.15%), Query Frame = 0

Query: 4   VLMERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVL 63
           +LMERQG+DD+HDCTIKLRVNP+KRRDKVYIGCGAGFGGDRPTAALKLLQRVK+LNYLVL
Sbjct: 19  MLMERQGEDDVHDCTIKLRVNPKKRRDKVYIGCGAGFGGDRPTAALKLLQRVKDLNYLVL 78

Query: 64  ECLAERTLADRYQVMLSGGDGYDSRNWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIE 123
           ECLAERTLADR+Q M SGGDGYDSRNWMKLLLPLA+KRNICIITNMGAMDPPGAQ+NVIE
Sbjct: 79  ECLAERTLADRHQAMSSGGDGYDSRNWMKLLLPLAVKRNICIITNMGAMDPPGAQQNVIE 138

Query: 124 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAPM 183
           IA SLGL+VSVAVAYEVSVKE GISTYLGAAPIV+CLEKYHPNVIITSRVADAALF+APM
Sbjct: 139 IASSLGLSVSVAVAYEVSVKESGISTYLGAAPIVKCLEKYHPNVIITSRVADAALFMAPM 198

Query: 184 VGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRKY 243
                                                                       
Sbjct: 199 ------------------------------------------------------------ 258

Query: 244 IQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTHP 303
                        VYELGWNWDD PRL+QG LAGHLLECGCQLTGGYFMHPG        
Sbjct: 259 -------------VYELGWNWDDFPRLSQGTLAGHLLECGCQLTGGYFMHPG-------- 318

Query: 304 NPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAEQ 363
                        DK+RSM  QQLL+ISLPYAE++CDGK  VAKAEETGGLLNFSTCAEQ
Sbjct: 319 -------------DKHRSMPFQQLLDISLPYAEIDCDGKVYVAKAEETGGLLNFSTCAEQ 378

Query: 364 LLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGWK 423
           LLYEVGDPSAYITPD+++                               L +  +DCGWK
Sbjct: 379 LLYEVGDPSAYITPDLVVDLSNVSFCSISSSKVFCSGAKPSIQVVPEKLLQLAPKDCGWK 438

Query: 424 GWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVED 483
           GWGEISYGGRECVLRAKAAEYLVRSWMEE L G+NQHIVSYIIGLDSLKAS NSS SVED
Sbjct: 439 GWGEISYGGRECVLRAKAAEYLVRSWMEEVLYGVNQHIVSYIIGLDSLKASINSS-SVED 498

Query: 484 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICYL 543
           IRLRMDGLF+ KEHALLFVREFTALYTNGPAGGGGIS                       
Sbjct: 499 IRLRMDGLFETKEHALLFVREFTALYTNGPAGGGGIS----------------------- 558

Query: 544 IESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQP 603
               TGYKKEIVLEKQL                   VGRE++FW+ GVKCT AV+LDS+P
Sbjct: 559 ----TGYKKEIVLEKQL-------------------VGREHVFWRMGVKCTKAVELDSRP 618

Query: 604 TDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRAG 663
           TDLR+DPA+  +SPRVTLPC I AYA+ PC  S  PETGHSPIPSGQ++ALYNVAHSRAG
Sbjct: 619 TDLREDPAKARTSPRVTLPCSIFAYADNPCASSSTPETGHSPIPSGQKVALYNVAHSRAG 648

Query: 664 DKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEHV 723
           DKGND+NFSV+PHYPSDIERL+MIITPEWV RVLS L NS+ F   DADKKRD+WV+EHV
Sbjct: 679 DKGNDMNFSVVPHYPSDIERLKMIITPEWVKRVLSSLQNSSTFHDLDADKKRDEWVNEHV 648

Query: 724 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDL+LNQQ+VLPP
Sbjct: 739 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLVLNQQVVLPP 648

BLAST of Lsi05G020240 vs. ExPASy TrEMBL
Match: A0A6J1IJ63 (uncharacterized protein LOC111474742 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111474742 PE=4 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 5.0e-286
Identity = 520/773 (67.27%), Postives = 564/773 (72.96%), Query Frame = 0

Query: 4   VLMERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVL 63
           +LMERQG+DD+HDCTIKLRVNP+KRRDKVYIGCGAGFGGDRPTAALKLLQRVK+LNYLVL
Sbjct: 1   MLMERQGEDDVHDCTIKLRVNPKKRRDKVYIGCGAGFGGDRPTAALKLLQRVKDLNYLVL 60

Query: 64  ECLAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNV 123
           ECLAERTLADR+Q M SGGDGYDSR  +WMKLLLPLA+KRNICIITNMGAMDPPGAQ+NV
Sbjct: 61  ECLAERTLADRHQAMSSGGDGYDSRIADWMKLLLPLAVKRNICIITNMGAMDPPGAQQNV 120

Query: 124 IEIAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLA 183
           IEIA SLGL+VSVAVAYEVSVKE GISTYLGAAPIV+CLEKYHPNVIITSRVADAALF+A
Sbjct: 121 IEIASSLGLSVSVAVAYEVSVKESGISTYLGAAPIVKCLEKYHPNVIITSRVADAALFMA 180

Query: 184 PMVGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYR 243
           PM                                                          
Sbjct: 181 PM---------------------------------------------------------- 240

Query: 244 KYIQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHT 303
                          VYELGWNWDD PRL+QG LAGHLLECGCQLTGGYFMHPG      
Sbjct: 241 ---------------VYELGWNWDDFPRLSQGTLAGHLLECGCQLTGGYFMHPG------ 300

Query: 304 HPNPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCA 363
                          DK+RSM  QQLL+ISLPYAE++CDGK  VAKAEETGGLLNFSTCA
Sbjct: 301 ---------------DKHRSMPFQQLLDISLPYAEIDCDGKVYVAKAEETGGLLNFSTCA 360

Query: 364 EQLLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCG 423
           EQLLYEVGDPSAYITPD+++                               L +  +DCG
Sbjct: 361 EQLLYEVGDPSAYITPDLVVDLSNVSFCSISSSKVFCSGAKPSIQVVPEKLLQLAPKDCG 420

Query: 424 WKGWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSV 483
           WKGWGEISYGGRECVLRAKAAEYLVRSWMEE L G+NQHIVSYIIGLDSLKAS NSS SV
Sbjct: 421 WKGWGEISYGGRECVLRAKAAEYLVRSWMEEVLYGVNQHIVSYIIGLDSLKASINSS-SV 480

Query: 484 EDIRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLIC 543
           EDIRLRMDGLF+ KEHALLFVREFTALYTNGPAGGGGIS                     
Sbjct: 481 EDIRLRMDGLFETKEHALLFVREFTALYTNGPAGGGGIS--------------------- 540

Query: 544 YLIESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDS 603
                 TGYKKEIVLEKQL                   VGRE++FW+ GVKCT AV+LDS
Sbjct: 541 ------TGYKKEIVLEKQL-------------------VGREHVFWRMGVKCTKAVELDS 600

Query: 604 QPTDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSR 663
           +PTDLR+DPA+  +SPRVTLPC I AYA+ PC  S  PETGHSPIPSGQ++ALYNVAHSR
Sbjct: 601 RPTDLREDPAKARTSPRVTLPCSIFAYADNPCASSSTPETGHSPIPSGQKVALYNVAHSR 632

Query: 664 AGDKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDE 723
           AGDKGND+NFSV+PHYPSDIERL+MIITPEWV RVLS L NS+ F   DADKKRD+WV+E
Sbjct: 661 AGDKGNDMNFSVVPHYPSDIERLKMIITPEWVKRVLSSLQNSSTFHDLDADKKRDEWVNE 632

Query: 724 HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDL+LNQQ+VLPP
Sbjct: 721 HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLVLNQQVVLPP 632

BLAST of Lsi05G020240 vs. ExPASy TrEMBL
Match: A0A6J1IGN9 (uncharacterized protein LOC111474742 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111474742 PE=4 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 5.0e-286
Identity = 520/773 (67.27%), Postives = 564/773 (72.96%), Query Frame = 0

Query: 4   VLMERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVL 63
           +LMERQG+DD+HDCTIKLRVNP+KRRDKVYIGCGAGFGGDRPTAALKLLQRVK+LNYLVL
Sbjct: 19  MLMERQGEDDVHDCTIKLRVNPKKRRDKVYIGCGAGFGGDRPTAALKLLQRVKDLNYLVL 78

Query: 64  ECLAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNV 123
           ECLAERTLADR+Q M SGGDGYDSR  +WMKLLLPLA+KRNICIITNMGAMDPPGAQ+NV
Sbjct: 79  ECLAERTLADRHQAMSSGGDGYDSRIADWMKLLLPLAVKRNICIITNMGAMDPPGAQQNV 138

Query: 124 IEIAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLA 183
           IEIA SLGL+VSVAVAYEVSVKE GISTYLGAAPIV+CLEKYHPNVIITSRVADAALF+A
Sbjct: 139 IEIASSLGLSVSVAVAYEVSVKESGISTYLGAAPIVKCLEKYHPNVIITSRVADAALFMA 198

Query: 184 PMVGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYR 243
           PM                                                          
Sbjct: 199 PM---------------------------------------------------------- 258

Query: 244 KYIQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHT 303
                          VYELGWNWDD PRL+QG LAGHLLECGCQLTGGYFMHPG      
Sbjct: 259 ---------------VYELGWNWDDFPRLSQGTLAGHLLECGCQLTGGYFMHPG------ 318

Query: 304 HPNPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCA 363
                          DK+RSM  QQLL+ISLPYAE++CDGK  VAKAEETGGLLNFSTCA
Sbjct: 319 ---------------DKHRSMPFQQLLDISLPYAEIDCDGKVYVAKAEETGGLLNFSTCA 378

Query: 364 EQLLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCG 423
           EQLLYEVGDPSAYITPD+++                               L +  +DCG
Sbjct: 379 EQLLYEVGDPSAYITPDLVVDLSNVSFCSISSSKVFCSGAKPSIQVVPEKLLQLAPKDCG 438

Query: 424 WKGWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSV 483
           WKGWGEISYGGRECVLRAKAAEYLVRSWMEE L G+NQHIVSYIIGLDSLKAS NSS SV
Sbjct: 439 WKGWGEISYGGRECVLRAKAAEYLVRSWMEEVLYGVNQHIVSYIIGLDSLKASINSS-SV 498

Query: 484 EDIRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLIC 543
           EDIRLRMDGLF+ KEHALLFVREFTALYTNGPAGGGGIS                     
Sbjct: 499 EDIRLRMDGLFETKEHALLFVREFTALYTNGPAGGGGIS--------------------- 558

Query: 544 YLIESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDS 603
                 TGYKKEIVLEKQL                   VGRE++FW+ GVKCT AV+LDS
Sbjct: 559 ------TGYKKEIVLEKQL-------------------VGREHVFWRMGVKCTKAVELDS 618

Query: 604 QPTDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSR 663
           +PTDLR+DPA+  +SPRVTLPC I AYA+ PC  S  PETGHSPIPSGQ++ALYNVAHSR
Sbjct: 619 RPTDLREDPAKARTSPRVTLPCSIFAYADNPCASSSTPETGHSPIPSGQKVALYNVAHSR 650

Query: 664 AGDKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDE 723
           AGDKGND+NFSV+PHYPSDIERL+MIITPEWV RVLS L NS+ F   DADKKRD+WV+E
Sbjct: 679 AGDKGNDMNFSVVPHYPSDIERLKMIITPEWVKRVLSSLQNSSTFHDLDADKKRDEWVNE 650

Query: 724 HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDL+LNQQ+VLPP
Sbjct: 739 HVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLVLNQQVVLPP 650

BLAST of Lsi05G020240 vs. NCBI nr
Match: XP_038900159.1 (uncharacterized protein LOC120087281 isoform X1 [Benincasa hispida] >XP_038900167.1 uncharacterized protein LOC120087281 isoform X1 [Benincasa hispida])

HSP 1 Score: 1038.1 bits (2683), Expect = 3.7e-299
Identity = 546/771 (70.82%), Postives = 570/771 (73.93%), Query Frame = 0

Query: 6   MERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 65
           MERQGKDDIHDCTIKLRVNPQK+RDKVYIGCGAGFGGDRPTAALKLLQRVK+LNYL+LEC
Sbjct: 1   MERQGKDDIHDCTIKLRVNPQKQRDKVYIGCGAGFGGDRPTAALKLLQRVKSLNYLILEC 60

Query: 66  LAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIE 125
           LAERTLADRYQVMLSGGDGYDSR  +WMKLLLPLAMKRNICIITNMGAMDPP AQRNVIE
Sbjct: 61  LAERTLADRYQVMLSGGDGYDSRIADWMKLLLPLAMKRNICIITNMGAMDPPAAQRNVIE 120

Query: 126 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAPM 185
           IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSR+ADAALFLAPM
Sbjct: 121 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRIADAALFLAPM 180

Query: 186 VGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRKY 245
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 246 IQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTHP 305
                        VYELGWNWDD PRLAQGILAGHLLECGCQLTGGYFMHPG        
Sbjct: 241 -------------VYELGWNWDDFPRLAQGILAGHLLECGCQLTGGYFMHPG-------- 300

Query: 306 NPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAEQ 365
                        DKYRSMS QQLLNISLPYAE+ECDG+ TVAKAEETGGLLNFSTCAEQ
Sbjct: 301 -------------DKYRSMSLQQLLNISLPYAEIECDGRITVAKAEETGGLLNFSTCAEQ 360

Query: 366 LLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGWK 425
           LLYEVGDPSAYITPDM++                               L +  +DCGWK
Sbjct: 361 LLYEVGDPSAYITPDMVVDFSNVSFCSISSSRVFCSGAKPSIQGTPEKLLQLAPKDCGWK 420

Query: 426 GWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVED 485
           GWGEISYGGRECVLRAKAA+YLVRSW+EE LIG+NQ IVSY IGLDSLKAS+NSS SVED
Sbjct: 421 GWGEISYGGRECVLRAKAADYLVRSWIEELLIGVNQDIVSYTIGLDSLKASNNSSTSVED 480

Query: 486 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICYL 545
           IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGIS                       
Sbjct: 481 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGIS----------------------- 540

Query: 546 IESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQP 605
               TGYKKEIVLEKQL                   VGRENIFWQTGVKCT AVKLDSQP
Sbjct: 541 ----TGYKKEIVLEKQL-------------------VGRENIFWQTGVKCTEAVKLDSQP 600

Query: 606 TDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRAG 665
            D R+DPAE  SSP+V LPCPISA AEKP  G FPPE GHSP+PS QEIALYNVAHSRAG
Sbjct: 601 IDARQDPAEARSSPQVMLPCPISASAEKPFMGFFPPEPGHSPVPSAQEIALYNVAHSRAG 631

Query: 666 DKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEHV 725
           DKGNDLNFSVIPHYPSDIERL+M+ITPEWV RVLSVLHNST FPSSDA+KKRD+WVDE V
Sbjct: 661 DKGNDLNFSVIPHYPSDIERLKMVITPEWVTRVLSVLHNSTQFPSSDANKKRDEWVDEDV 631

Query: 726 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           KVEIYEV+GIHSLNVVVRNILDGGVNCSRRIDRHGK +SDLILNQ IVLPP
Sbjct: 721 KVEIYEVEGIHSLNVVVRNILDGGVNCSRRIDRHGKAISDLILNQHIVLPP 631

BLAST of Lsi05G020240 vs. NCBI nr
Match: XP_023539935.1 (uncharacterized protein LOC111800461 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1007.3 bits (2603), Expect = 6.9e-290
Identity = 524/772 (67.88%), Postives = 568/772 (73.58%), Query Frame = 0

Query: 4   VLMERQGK-DDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLV 63
           +LMERQG+ DD+HDCTIKLR+NP+KRRDKVYIGCGAGFGGDRPTAALKLLQRVK+LNYLV
Sbjct: 1   MLMERQGEDDDVHDCTIKLRLNPKKRRDKVYIGCGAGFGGDRPTAALKLLQRVKDLNYLV 60

Query: 64  LECLAERTLADRYQVMLSGGDGYDSRNWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVI 123
           LECLAERTLADR+Q M SGGDGYDSRNWMKLLLPLA++RNICIITNMGAMDPPGAQ+NVI
Sbjct: 61  LECLAERTLADRHQAMSSGGDGYDSRNWMKLLLPLAVERNICIITNMGAMDPPGAQQNVI 120

Query: 124 EIAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAP 183
           EIA SLGL+VSVAVAYEVSVKE GISTYLGAAPIVECLEKYHPNVIITSRVADAALF+AP
Sbjct: 121 EIASSLGLSVSVAVAYEVSVKESGISTYLGAAPIVECLEKYHPNVIITSRVADAALFMAP 180

Query: 184 MVGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRK 243
           M                                                           
Sbjct: 181 M----------------------------------------------------------- 240

Query: 244 YIQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTH 303
                         VYELGWNWDD PRL+QG LAGHLLECGCQLTGGYFMHPG       
Sbjct: 241 --------------VYELGWNWDDFPRLSQGTLAGHLLECGCQLTGGYFMHPG------- 300

Query: 304 PNPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAE 363
                         DK+RSM  QQLL+ISLPYAE++CDGK  VAKAEETGGLLNFSTCAE
Sbjct: 301 --------------DKHRSMHFQQLLDISLPYAEIDCDGKVYVAKAEETGGLLNFSTCAE 360

Query: 364 QLLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGW 423
           QLLYEVGDPSAYITPD+++                               L +  +DCGW
Sbjct: 361 QLLYEVGDPSAYITPDLVVDLSNVSFCSISSSKVFCSGAKPSIQVVPEKLLQLAPKDCGW 420

Query: 424 KGWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVE 483
           KGWGEISYGGRECVLRAKAAEYLVRSWMEE L G+NQHIVSYIIGLDSLKAS NSS SVE
Sbjct: 421 KGWGEISYGGRECVLRAKAAEYLVRSWMEEVLNGVNQHIVSYIIGLDSLKASINSS-SVE 480

Query: 484 DIRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICY 543
           DIRLRMDGLF+ KEH LLFVREFTALYTNGPAGGGGIS                      
Sbjct: 481 DIRLRMDGLFETKEHTLLFVREFTALYTNGPAGGGGIS---------------------- 540

Query: 544 LIESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQ 603
                TGYKKEI+LEKQL                   VGRE++FW+TGVKCT AV+LDS+
Sbjct: 541 -----TGYKKEILLEKQL-------------------VGREHVFWRTGVKCTKAVELDSR 600

Query: 604 PTDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRA 663
           PTDLR+DPA+  +SPRVTLPCPI AYA+ PC GS PPETGHSPIPSGQ++ALYNVAHSRA
Sbjct: 601 PTDLREDPAKARTSPRVTLPCPIFAYADNPCAGSSPPETGHSPIPSGQKVALYNVAHSRA 631

Query: 664 GDKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEH 723
           GDKGND+NFSVIPHYPSDIERL+MIITPEWV RVLS L NS+ FP  DADKKRD+W+DEH
Sbjct: 661 GDKGNDMNFSVIPHYPSDIERLKMIITPEWVKRVLSSLQNSSTFPDLDADKKRDEWIDEH 631

Query: 724 VKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           VKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDL+LNQQ+VLPP
Sbjct: 721 VKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLVLNQQVVLPP 631

BLAST of Lsi05G020240 vs. NCBI nr
Match: XP_004134329.1 (uncharacterized protein LOC101212841 isoform X2 [Cucumis sativus] >XP_011650759.1 uncharacterized protein LOC101212841 isoform X2 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.5e-289
Identity = 533/771 (69.13%), Postives = 561/771 (72.76%), Query Frame = 0

Query: 6   MERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 65
           ME  G+ DIHDCTIKLRVNPQK+RDKV IGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC
Sbjct: 1   MEGHGQADIHDCTIKLRVNPQKQRDKVCIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 60

Query: 66  LAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIE 125
           LAERTLAD YQVMLSGGDGYD R  +WMKLLLPLAMKRNICIITNMGAMDPP AQ+NVIE
Sbjct: 61  LAERTLADHYQVMLSGGDGYDPRIADWMKLLLPLAMKRNICIITNMGAMDPPAAQQNVIE 120

Query: 126 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAPM 185
           +AGSLGLNVSVAVAYE SVKE GISTY+G APIVECLEKYHPNVIITSRVADAALFLAPM
Sbjct: 121 VAGSLGLNVSVAVAYEGSVKESGISTYMGGAPIVECLEKYHPNVIITSRVADAALFLAPM 180

Query: 186 VGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRKY 245
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 246 IQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTHP 305
                        VYELGWNWDD P LAQGILAGHLLECGCQLTGGYFMHPG        
Sbjct: 241 -------------VYELGWNWDDFPLLAQGILAGHLLECGCQLTGGYFMHPG-------- 300

Query: 306 NPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAEQ 365
                        DKYRSMS QQLLNISLPYAEVECDGK TVAK EE+GGLLNFSTCAEQ
Sbjct: 301 -------------DKYRSMSFQQLLNISLPYAEVECDGKLTVAKPEESGGLLNFSTCAEQ 360

Query: 366 LLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGWK 425
           LLYE+G+PSAYITPD+++                               L +  +DCGWK
Sbjct: 361 LLYEIGNPSAYITPDLVVDFSNVSFCSISSSRVLCSGAKPSIQGVPEKLLQLAPKDCGWK 420

Query: 426 GWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVED 485
           GWGEISYGGRECVLRAKAAEYLVRSWMEE LIGIN+HIVSY IGLDSLKASSN SN VED
Sbjct: 421 GWGEISYGGRECVLRAKAAEYLVRSWMEELLIGINRHIVSYTIGLDSLKASSNGSNCVED 480

Query: 486 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICYL 545
           IRLRMDGLF+QKEHALLFV+EFTALYTNGPAGGGGIS                       
Sbjct: 481 IRLRMDGLFEQKEHALLFVKEFTALYTNGPAGGGGIS----------------------- 540

Query: 546 IESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQP 605
               TGYKKEIVLEKQL                   VGRENIFWQT V CT AVKLDSQ 
Sbjct: 541 ----TGYKKEIVLEKQL-------------------VGRENIFWQTEVTCTEAVKLDSQS 600

Query: 606 TDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRAG 665
           TDL+KDPAE CSSPRVTLPCPIS +A++ CTGS PPE GHSPIPSGQEIALYNVAHSRAG
Sbjct: 601 TDLQKDPAEACSSPRVTLPCPISDHADELCTGSLPPEMGHSPIPSGQEIALYNVAHSRAG 631

Query: 666 DKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEHV 725
           DKGNDLNFS+IPH PSDIERL+MIITPEWVMRVLSVLHNST F SS+AD+KR++WV E V
Sbjct: 661 DKGNDLNFSLIPHCPSDIERLKMIITPEWVMRVLSVLHNSTRFHSSNADEKRNEWVSEDV 631

Query: 726 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDLILNQ IVLPP
Sbjct: 721 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLILNQLIVLPP 631

BLAST of Lsi05G020240 vs. NCBI nr
Match: XP_008438065.1 (PREDICTED: uncharacterized protein LOC103483286 [Cucumis melo] >XP_008438066.1 PREDICTED: uncharacterized protein LOC103483286 [Cucumis melo])

HSP 1 Score: 1003.8 bits (2594), Expect = 7.7e-289
Identity = 531/771 (68.87%), Postives = 562/771 (72.89%), Query Frame = 0

Query: 6   MERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 65
           MER  + DIHDCTIKLRVNP+K+RDKV IGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC
Sbjct: 1   MERHSQADIHDCTIKLRVNPKKQRDKVCIGCGAGFGGDRPTAALKLLQRVKNLNYLVLEC 60

Query: 66  LAERTLADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIE 125
           LAERTLAD YQVMLSGGDGYDSR   WMKLLLPL+MKRNICIITNMGAMDP  AQ+ VIE
Sbjct: 61  LAERTLADHYQVMLSGGDGYDSRIAEWMKLLLPLSMKRNICIITNMGAMDPLAAQQKVIE 120

Query: 126 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAPM 185
           +AGSLGLNVSVAVAYE SVKE GISTY+G APIVECLEKYHPNVIITSRVADAALFLAPM
Sbjct: 121 VAGSLGLNVSVAVAYEGSVKESGISTYMGGAPIVECLEKYHPNVIITSRVADAALFLAPM 180

Query: 186 VGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRKY 245
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 246 IQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTHP 305
                        VYELGWNWDD P LAQGILAGHLLECGCQLTGGYFMHPG        
Sbjct: 241 -------------VYELGWNWDDFPLLAQGILAGHLLECGCQLTGGYFMHPG-------- 300

Query: 306 NPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAEQ 365
                        DKYRSMS QQLLNISLPYAEVECDGK TVAK EE+GGLLNFSTCAEQ
Sbjct: 301 -------------DKYRSMSFQQLLNISLPYAEVECDGKLTVAKPEESGGLLNFSTCAEQ 360

Query: 366 LLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGWK 425
           LLYE+GDPSAYITPD+++                               L +  +DCGWK
Sbjct: 361 LLYEIGDPSAYITPDLVVDFSNVSFCSISSSRVVCSGAKPSIQGVPEKLLQLAPKDCGWK 420

Query: 426 GWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVED 485
           GWGEISYGGRECVLRAKAAEYLVRSWMEE LIGIN+HIVSY IGLDSLKASSNSSN +ED
Sbjct: 421 GWGEISYGGRECVLRAKAAEYLVRSWMEELLIGINEHIVSYTIGLDSLKASSNSSNCIED 480

Query: 486 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICYL 545
           IRLRMDGLF+QKEHALLFV+EFTALYTNGPAGGGGIS                       
Sbjct: 481 IRLRMDGLFEQKEHALLFVKEFTALYTNGPAGGGGIS----------------------- 540

Query: 546 IESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQP 605
               TGYKKEIVLEKQL                   VGRENIFWQT VKC+ AVKLDSQ 
Sbjct: 541 ----TGYKKEIVLEKQL-------------------VGRENIFWQTEVKCSEAVKLDSQS 600

Query: 606 TDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRAG 665
           TDL+KDPAE CSSPRVTLPCPIS++AEK CTGSFPPETGHSPIPSGQEIALY+VAHSRAG
Sbjct: 601 TDLQKDPAEACSSPRVTLPCPISSHAEKLCTGSFPPETGHSPIPSGQEIALYDVAHSRAG 631

Query: 666 DKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEHV 725
           DKGNDLNFS+IPHYPSDIERL+MIITPEWVMRVLS LHN T F SS+A +KR++WV+E V
Sbjct: 661 DKGNDLNFSLIPHYPSDIERLKMIITPEWVMRVLSGLHNLTRFHSSNAGEKRNEWVNEDV 631

Query: 726 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           KVEIYEVK IHSLNVVVRNILDGGVNCSRRIDRHGKT+SDLILNQ IVLPP
Sbjct: 721 KVEIYEVKSIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLILNQLIVLPP 631

BLAST of Lsi05G020240 vs. NCBI nr
Match: XP_022975426.1 (uncharacterized protein LOC111474742 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1000.3 bits (2585), Expect = 8.5e-288
Identity = 521/771 (67.57%), Postives = 564/771 (73.15%), Query Frame = 0

Query: 4   VLMERQGKDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVL 63
           +LMERQG+DD+HDCTIKLRVNP+KRRDKVYIGCGAGFGGDRPTAALKLLQRVK+LNYLVL
Sbjct: 19  MLMERQGEDDVHDCTIKLRVNPKKRRDKVYIGCGAGFGGDRPTAALKLLQRVKDLNYLVL 78

Query: 64  ECLAERTLADRYQVMLSGGDGYDSRNWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIE 123
           ECLAERTLADR+Q M SGGDGYDSRNWMKLLLPLA+KRNICIITNMGAMDPPGAQ+NVIE
Sbjct: 79  ECLAERTLADRHQAMSSGGDGYDSRNWMKLLLPLAVKRNICIITNMGAMDPPGAQQNVIE 138

Query: 124 IAGSLGLNVSVAVAYEVSVKEPGISTYLGAAPIVECLEKYHPNVIITSRVADAALFLAPM 183
           IA SLGL+VSVAVAYEVSVKE GISTYLGAAPIV+CLEKYHPNVIITSRVADAALF+APM
Sbjct: 139 IASSLGLSVSVAVAYEVSVKESGISTYLGAAPIVKCLEKYHPNVIITSRVADAALFMAPM 198

Query: 184 VGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGPQPRSYRKY 243
                                                                       
Sbjct: 199 ------------------------------------------------------------ 258

Query: 244 IQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPGLLLLHTHP 303
                        VYELGWNWDD PRL+QG LAGHLLECGCQLTGGYFMHPG        
Sbjct: 259 -------------VYELGWNWDDFPRLSQGTLAGHLLECGCQLTGGYFMHPG-------- 318

Query: 304 NPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLLNFSTCAEQ 363
                        DK+RSM  QQLL+ISLPYAE++CDGK  VAKAEETGGLLNFSTCAEQ
Sbjct: 319 -------------DKHRSMPFQQLLDISLPYAEIDCDGKVYVAKAEETGGLLNFSTCAEQ 378

Query: 364 LLYEVGDPSAYITPDMLI-------------------------------LDVTYQDCGWK 423
           LLYEVGDPSAYITPD+++                               L +  +DCGWK
Sbjct: 379 LLYEVGDPSAYITPDLVVDLSNVSFCSISSSKVFCSGAKPSIQVVPEKLLQLAPKDCGWK 438

Query: 424 GWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKASSNSSNSVED 483
           GWGEISYGGRECVLRAKAAEYLVRSWMEE L G+NQHIVSYIIGLDSLKAS NSS SVED
Sbjct: 439 GWGEISYGGRECVLRAKAAEYLVRSWMEEVLYGVNQHIVSYIIGLDSLKASINSS-SVED 498

Query: 484 IRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDLFFDVYPLLICYL 543
           IRLRMDGLF+ KEHALLFVREFTALYTNGPAGGGGIS                       
Sbjct: 499 IRLRMDGLFETKEHALLFVREFTALYTNGPAGGGGIS----------------------- 558

Query: 544 IESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGVKCTVAVKLDSQP 603
               TGYKKEIVLEKQL                   VGRE++FW+ GVKCT AV+LDS+P
Sbjct: 559 ----TGYKKEIVLEKQL-------------------VGREHVFWRMGVKCTKAVELDSRP 618

Query: 604 TDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQEIALYNVAHSRAG 663
           TDLR+DPA+  +SPRVTLPC I AYA+ PC  S  PETGHSPIPSGQ++ALYNVAHSRAG
Sbjct: 619 TDLREDPAKARTSPRVTLPCSIFAYADNPCASSSTPETGHSPIPSGQKVALYNVAHSRAG 648

Query: 664 DKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDADKKRDDWVDEHV 723
           DKGND+NFSV+PHYPSDIERL+MIITPEWV RVLS L NS+ F   DADKKRD+WV+EHV
Sbjct: 679 DKGNDMNFSVVPHYPSDIERLKMIITPEWVKRVLSSLQNSSTFHDLDADKKRDEWVNEHV 648

Query: 724 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIVLPP 744
           KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKT+SDL+LNQQ+VLPP
Sbjct: 739 KVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTISDLVLNQQVVLPP 648

BLAST of Lsi05G020240 vs. TAIR 10
Match: AT1G01770.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1446 (InterPro:IPR010839); Has 1597 Blast hits to 1509 proteins in 306 species: Archae - 4; Bacteria - 843; Metazoa - 22; Fungi - 131; Plants - 31; Viruses - 0; Other Eukaryotes - 566 (source: NCBI BLink). )

HSP 1 Score: 687.6 bits (1773), Expect = 1.1e-197
Identity = 392/781 (50.19%), Postives = 467/781 (59.80%), Query Frame = 0

Query: 11  KDDIHDCTIKLRVNPQKRRDKVYIGCGAGFGGDRPTAALKLLQRVKNLNYLVLECLAERT 70
           K+ + DC I LR NP++RR+ VY+GCGAGFGGDRP AALKLLQRV+ LNYLVLECLAERT
Sbjct: 7   KEILCDCVINLRENPKRRRETVYVGCGAGFGGDRPLAALKLLQRVEELNYLVLECLAERT 66

Query: 71  LADRYQVMLSGGDGYDSR--NWMKLLLPLAMKRNICIITNMGAMDPPGAQRNVIEIAGSL 130
           LADR+  M SGG GYD R   WM+LLLPLA++R  CIITNMGA+DP GAQ+ V+E+AG L
Sbjct: 67  LADRWLSMASGGLGYDPRVSEWMQLLLPLAVERGTCIITNMGAIDPSGAQKKVLEVAGEL 126

Query: 131 GLNVSVAVAYEVSVK-------------EPGISTYLGAAPIVECLEKYHPNVIITSRVAD 190
           GL +SVAVA+EV  +               G STYLGAAPIVECLEKY PNVIITSRVAD
Sbjct: 127 GLTISVAVAHEVHFETGSGSSFGGQYCSAGGTSTYLGAAPIVECLEKYQPNVIITSRVAD 186

Query: 191 AALFLAPMVGSDFYMMLAQSFRSDSYLCENINHFREVALCLLWGRENFMDFRDFSLFFGP 250
           AALFLAPM                                                    
Sbjct: 187 AALFLAPM---------------------------------------------------- 246

Query: 251 QPRSYRKYIQLIVIEGMLLEFVYELGWNWDDLPRLAQGILAGHLLECGCQLTGGYFMHPG 310
                                VYELGWNW+DL  LAQG LAGHLLECGCQLTGGYFMHPG
Sbjct: 247 ---------------------VYELGWNWNDLELLAQGTLAGHLLECGCQLTGGYFMHPG 306

Query: 311 LLLLHTHPNPNSLYVEHGTHRDKYRSMSSQQLLNISLPYAEVECDGKTTVAKAEETGGLL 370
                                D+YR M+   L ++SLPYAE+  DGK  V+K E +GG+L
Sbjct: 307 ---------------------DQYRDMAFPLLQDLSLPYAEIGYDGKVCVSKVEGSGGIL 366

Query: 371 NFSTCAEQLLYEVGDPSAYITPDMLI--------------------------------LD 430
           N STCAEQLLYE+ DPSAYITPD++I                                L 
Sbjct: 367 NTSTCAEQLLYEIADPSAYITPDVVIDIRGVSFLPLSDCKVQCSGAKPSSNTSVPEKLLR 426

Query: 431 VTYQDCGWKGWGEISYGGRECVLRAKAAEYLVRSWMEEQLIGINQHIVSYIIGLDSLKAS 490
           +  ++CGWKGWGEISYGG   + RAKA+E+LVRSWMEE + G+N  I+SY+IG+DSLKA+
Sbjct: 427 LIPKECGWKGWGEISYGGNGSIQRAKASEFLVRSWMEETIPGVNHCILSYVIGVDSLKAT 486

Query: 491 SNSSNSVE---DIRLRMDGLFKQKEHALLFVREFTALYTNGPAGGGGISWTVAFVSTSDL 550
           SN + S +   DIRLRMDGLFK KEHA+   +EFTALYTNGPAGGGGIS           
Sbjct: 487 SNGTESWQSCGDIRLRMDGLFKLKEHAVQLTKEFTALYTNGPAGGGGIS----------- 546

Query: 551 FFDVYPLLICYLIESITGYKKEIVLEKQLSANVFLFHNELFSYPSFFNVGRENIFWQTGV 610
                           TG+K EIVLEK+L                   V RE++ W+TG+
Sbjct: 547 ----------------TGHKMEIVLEKRL-------------------VSRESVMWKTGL 606

Query: 611 KCTVAVKLDSQPTDLRKDPAEECSSPRVTLPCPISAYAEKPCTGSFPPETGHSPIPSGQE 670
                     Q T+  +    E  SP      P          G +     HSP PSGQ+
Sbjct: 607 ----------QHTNTSEPETSEHHSPEKMPKLPKENPKNLTMRG-YQSGFHHSPAPSGQK 631

Query: 671 IALYNVAHSRAGDKGNDLNFSVIPHYPSDIERLRMIITPEWVMRVLSVLHNSTLFPSSDA 730
           I LY+VAHSRAGDKGND+NFS+IPHY  D+ERL++IITP+WV  V+SVL +++ F   DA
Sbjct: 667 IPLYSVAHSRAGDKGNDINFSIIPHYSPDVERLKLIITPQWVKHVMSVLLSTSSFLELDA 631

Query: 731 DKKRDDWVDEHVKVEIYEVKGIHSLNVVVRNILDGGVNCSRRIDRHGKTLSDLILNQQIV 742
                  +DE+V VEIY+V+GIH++NVVVRNILDGGVNCSRRIDRHGKT+SDLIL QQ+V
Sbjct: 727 KP-----MDENVSVEIYDVEGIHAMNVVVRNILDGGVNCSRRIDRHGKTISDLILCQQVV 631