Lsi05G019860 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G019860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlyoxylate/hydroxypyruvate reductase HPR3-like
Locationchr05: 26958029 .. 26960003 (+)
RNA-Seq ExpressionLsi05G019860
SyntenyLsi05G019860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGCCGAAGACCAGGCGAAGGAGCTTTCTCAAGTGTTGGTTCTAGGTCCCCCTTCTATATTTCCATATCTGGAATCCCAATTCTCAAATAGATTCCATTTCCTAAAACCATGGCTTTCCAAACTCTCTCTAACTCAATTTCTCACCTCTTTTGCCCAATCCACTCAAACCTTGCTTATCCGAGGCGGTGGCAGTACCCAACTCACCTCCGCCATTCTCGACTGCCTCCCCTCGCTCAAGCTCGTCGTCACTAGCAGCGTCGGTGTCGATCACTTGGACTTGCCGGAATTACGCCGGCGTGGGGTGGCTATTGCCTATGCAGGAAACGTCTTCTCCGAAGATGCCGCCGATATGGCGGTTGGATTACTAATCGACGTTCTTAGGAAAGTGTCGGCGGGAGATCGGTTCGTGAGGCAAGGGCTTTGGCCAAGGAAAGGGGATTTTCCTCCCGGATTAAAGGTCCAGCACTGATTGCAATTCCGTCCTTGTTTTTCGCTATCCACATCATTGCCACTTTTTTTTATTATTTATTTTTTAAAAAAAAATCCTATGGTTGAAGACTTTCTAACATTTTTCAGTTACAACTTTTTTTAAAAAAATTAAAATTAGTTTGTGTAAACATTCATTGCATCATACTTCGTGAATTACAAAATACTTGGTGAGTTACTAGAAATTTGGGGAAGAATTAGGTTTCCTAAAGAAGAAGAAAATTAGAAAAATTTGCCAAACTTTTAAAGTTTTGAAGAAATTATTTAACTTTCTCAACATATTTAAAAAAAAACAAAAAATCTAAGAATACTAATAATAATATCTTTTTTAACTTAAATTAGAAGTAAAACAACTTAATTATTAATCTAAATAAATTTTATAAATTTTGTTACTAATAGAAATATCCGTTGACATTGATATATCTATCGATATGTAAAATTAAAATCTTGATATAGGTATCAATATTGATATTTTAATCTTCAGTTCCTACCAACTTAATTTTAAAAGATGTTGAACTATAATAAACTAAATTGAAACTTTTTAAAACCAAATTGAAAGAAAAACAACCAAACAGGATTGGCTAATATATATGTATAGAAAGAGAGCCGATCTCTGTATACAAAGTTAACAGATTCTGATATTCCTAAAGCTAATTAAATGATTACTCATGGAAAAGGTGTTGTTTGATTTGGATGAATTTTGGAAAATTTTGCAGCTGAGCGGCAAGCGGATTGGGATCGTTGGATTGGGGAAAATAGGCTATGAAGTTGCCAAGAGGCTGGAGGGATTTGGCTGCAAAGTCTCATATAACTCAAGGACTAAAAAGTCAATGGCACAATATTCCTACTATTCCAATGTATATGAACTTGCAGCCAACTGTGAAGCACTCATCATTTGTTGTGCGCTGACTGAAGAAACCTACCATCTAATTAACAAGGAGGTGATGCTTGCATTAGGAAAAGATGGAGTGATAGTCAATATTGGCCGTGGTGCGATCATCGACGAGAAGGAGATGATTCGCTGTTTGATTCAGGGGGAGATTGGTGGAGCTGGACTAGATGTGTTTGAGAATGAACCTAAAATTCCTGAAGAGCTATTTAATCTTGATAATGTTGTTTTGTCACCACATGTTGCTGTTATGACATATGAATCTTCAATGGAATTGTCTAAGTTGGTGGTGGACAACTTGGAGGCATTCTTCTCAAACAAACCTCTTGTGTCTCCCTTTGTGGATTAGTTGTGGTGGAGTTTAGTTTTTGCTGAGAAAAGGAGGGCCTTCGAAAACTTGACAACAGAGCCACCCTAACGTTTTAGTGTATTCGACTCTTTTTTTGACTAAACAATATAAGGTTTAGCTACATGCAAGTTGACATATTCAAACATTTTTCTTTCTTTTTTACAGCCATTGAGACATACAATTTGAAGTGTCTACAGATTCTTATATCTCAAATTATGATGAAAATAAAGCTCTTAAAATGCGGAA

mRNA sequence

ATGACAGCCGAAGACCAGGCGAAGGAGCTTTCTCAAGTGTTGGTTCTAGGTCCCCCTTCTATATTTCCATATCTGGAATCCCAATTCTCAAATAGATTCCATTTCCTAAAACCATGGCTTTCCAAACTCTCTCTAACTCAATTTCTCACCTCTTTTGCCCAATCCACTCAAACCTTGCTTATCCGAGGCGGTGGCAGTACCCAACTCACCTCCGCCATTCTCGACTGCCTCCCCTCGCTCAAGCTCGTCGTCACTAGCAGCGTCGGTGTCGATCACTTGGACTTGCCGGAATTACGCCGGCGTGGGGTGGCTATTGCCTATGCAGGAAACGTCTTCTCCGAAGATGCCGCCGATATGGCGGTTGGATTACTAATCGACGTTCTTAGGAAAGTGTCGGCGGGAGATCGGTTCGTGAGGCAAGGGCTTTGGCCAAGGAAAGGGGATTTTCCTCCCGGATTAAAGCTGAGCGGCAAGCGGATTGGGATCGTTGGATTGGGGAAAATAGGCTATGAAGTTGCCAAGAGGCTGGAGGGATTTGGCTGCAAAGTCTCATATAACTCAAGGACTAAAAAGTCAATGGCACAATATTCCTACTATTCCAATGTATATGAACTTGCAGCCAACTGTGAAGCACTCATCATTTGTTGTGCGCTGACTGAAGAAACCTACCATCTAATTAACAAGGAGGTGATGCTTGCATTAGGAAAAGATGGAGTGATAGTCAATATTGGCCGTGGTGCGATCATCGACGAGAAGGAGATGATTCGCTGTTTGATTCAGGGGGAGATTGGTGGAGCTGGACTAGATGTGTTTGAGAATGAACCTAAAATTCCTGAAGAGCTATTTAATCTTGATAATGTTGTTTTGTCACCACATGTTGCTGTTATGACATATGAATCTTCAATGGAATTGTCTAAGTTGGTGGTGGACAACTTGGAGGCATTCTTCTCAAACAAACCTCTTGTGTCTCCCTTTGTGGATTAGTTGTGGTGGAGTTTAGTTTTTGCTGAGAAAAGGAGGGCCTTCGAAAACTTGACAACAGAGCCACCCTAACGTTTTAGTGTATTCGACTCTTTTTTTGACTAAACAATATAAGGTTTAGCTACATGCAAGTTGACATATTCAAACATTTTTCTTTCTTTTTTACAGCCATTGAGACATACAATTTGAAGTGTCTACAGATTCTTATATCTCAAATTATGATGAAAATAAAGCTCTTAAAATGCGGAA

Coding sequence (CDS)

ATGACAGCCGAAGACCAGGCGAAGGAGCTTTCTCAAGTGTTGGTTCTAGGTCCCCCTTCTATATTTCCATATCTGGAATCCCAATTCTCAAATAGATTCCATTTCCTAAAACCATGGCTTTCCAAACTCTCTCTAACTCAATTTCTCACCTCTTTTGCCCAATCCACTCAAACCTTGCTTATCCGAGGCGGTGGCAGTACCCAACTCACCTCCGCCATTCTCGACTGCCTCCCCTCGCTCAAGCTCGTCGTCACTAGCAGCGTCGGTGTCGATCACTTGGACTTGCCGGAATTACGCCGGCGTGGGGTGGCTATTGCCTATGCAGGAAACGTCTTCTCCGAAGATGCCGCCGATATGGCGGTTGGATTACTAATCGACGTTCTTAGGAAAGTGTCGGCGGGAGATCGGTTCGTGAGGCAAGGGCTTTGGCCAAGGAAAGGGGATTTTCCTCCCGGATTAAAGCTGAGCGGCAAGCGGATTGGGATCGTTGGATTGGGGAAAATAGGCTATGAAGTTGCCAAGAGGCTGGAGGGATTTGGCTGCAAAGTCTCATATAACTCAAGGACTAAAAAGTCAATGGCACAATATTCCTACTATTCCAATGTATATGAACTTGCAGCCAACTGTGAAGCACTCATCATTTGTTGTGCGCTGACTGAAGAAACCTACCATCTAATTAACAAGGAGGTGATGCTTGCATTAGGAAAAGATGGAGTGATAGTCAATATTGGCCGTGGTGCGATCATCGACGAGAAGGAGATGATTCGCTGTTTGATTCAGGGGGAGATTGGTGGAGCTGGACTAGATGTGTTTGAGAATGAACCTAAAATTCCTGAAGAGCTATTTAATCTTGATAATGTTGTTTTGTCACCACATGTTGCTGTTATGACATATGAATCTTCAATGGAATTGTCTAAGTTGGTGGTGGACAACTTGGAGGCATTCTTCTCAAACAAACCTCTTGTGTCTCCCTTTGTGGATTAG

Protein sequence

MTAEDQAKELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLVVDNLEAFFSNKPLVSPFVD
Homology
BLAST of Lsi05G019860 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.5e-90
Identity = 168/320 (52.50%), Postives = 228/320 (71.25%), Query Frame = 0

Query: 6   QAKELSQVLVLGPPSIFPYLESQFSNRFH-FLKPWLSKLSLTQFLTSFAQSTQTLLIRGG 65
           ++ E   VL+  PPS+  +++   +  F   +    S  SL  F    A S +  +I   
Sbjct: 3   ESSEPPVVLLHRPPSL-TFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI--S 62

Query: 66  GSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLL 125
           G   +T  +L  LPSL+++V +SVG+DH+DL   +RRG+ I  AGN FS+D AD AVGLL
Sbjct: 63  GRLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLL 122

Query: 126 IDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVS 185
           I VLR++ A DR+VR G W + GDF  G K+SGKR+GIVGLG IG  VAKRLE FGC +S
Sbjct: 123 ISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVIS 182

Query: 186 YNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIG 245
           YNSR++K  + Y YYS++  LA N + L++CC+LT+ET+H++N+EVM  LGKDGV++N+G
Sbjct: 183 YNSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVG 242

Query: 246 RGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMEL 305
           RG +IDEKEM++CL+ G IGGAGLDVFENEP +P+ELF LDNVVLSPH AV T  S   +
Sbjct: 243 RGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNV 302

Query: 306 SKLVVDNLEAFFSNKPLVSP 325
           +++ + NL+AFFSN+PL+SP
Sbjct: 303 AQIALANLKAFFSNRPLLSP 319

BLAST of Lsi05G019860 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.4e-74
Identity = 137/304 (45.07%), Postives = 200/304 (65.79%), Query Frame = 0

Query: 23  PYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQLTSAILDCLPSLKL 82
           PYLE +   RF   + W    S        + S + ++  G       + +++ LP +++
Sbjct: 14  PYLEQELDKRFKLFRFW-DFPSANDLFREHSNSIRAVV--GNSFIGADAQMIEALPKMEI 73

Query: 83  VVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVLRKVSAGDRFVRQGL 142
           V + SVG+D +DL   + +G+ +    +V +ED AD+A+ L++  LR++   DR+VR G 
Sbjct: 74  VSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSGS 133

Query: 143 WPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSRTKKSMAQYSYYSNV 202
           W +KGDF    K +GK +GI+GLG+IG  +AKR EGF C +SY+SRT+K    Y YY +V
Sbjct: 134 W-KKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPSV 193

Query: 203 YELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAIIDEKEMIRCLIQGE 262
            ELA+NC+ L++ CALT ET H+IN+EV+ ALG  GV++NIGRG  +DE E++  L++G 
Sbjct: 194 VELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGR 253

Query: 263 IGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLVVDNLEAFFSNKPLV 322
           +GGAGLDVFENEP +PEEL  +DNVVL PHV   T E+  +++ LV+ NLEA F NKPL+
Sbjct: 254 LGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLL 313

Query: 323 SPFV 327
           +P V
Sbjct: 314 TPVV 313

BLAST of Lsi05G019860 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 2.2e-73
Identity = 143/314 (45.54%), Postives = 211/314 (67.20%), Query Frame = 0

Query: 13  VLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQLTSA 72
           VL++ P S   YLE +   RF   + W ++ +   FL   A+S + ++  G  +    + 
Sbjct: 6   VLMMCPMS--TYLEQELDKRFKLFRYW-TQPAQRDFLALQAESIRAVV--GNSNAGADAE 65

Query: 73  ILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVLRKVS 132
           ++D LP L++V + SVG+D +DL +   +GV +    +V ++D AD+A+GL++ VLR++ 
Sbjct: 66  LIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRIC 125

Query: 133 AGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSRTKKS 192
             D++VR+G W + GDF    K SGKR+GI+GLG+IG  VA+R E F C +SY SR+KK 
Sbjct: 126 ECDKYVRRGAW-KFGDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKP 185

Query: 193 MAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAIIDEK 252
              Y+YY +V ELA+N + L++ C LT ET H+IN+EV+ ALG  GV++NIGRG  +DE 
Sbjct: 186 NTNYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEP 245

Query: 253 EMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLVVDNL 312
           E++  L++G +GGAGLDVFE EP++PE+LF L+NVVL PHV   T E+   ++ LVV NL
Sbjct: 246 ELVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNL 305

Query: 313 EAFFSNKPLVSPFV 327
           EA FS KPL++P V
Sbjct: 306 EAHFSGKPLLTPVV 313

BLAST of Lsi05G019860 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.5e-69
Identity = 137/314 (43.63%), Postives = 206/314 (65.61%), Query Frame = 0

Query: 13  VLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQLTSA 72
           VL++ P S   YLE++   RF+ L+ W S    +  L +   S + ++  G  S    + 
Sbjct: 6   VLMMCPMS--SYLENELEKRFNLLRFWTSP-EKSVLLETHRNSIRAVV--GNASAGADAQ 65

Query: 73  ILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVLRKVS 132
           ++  LP+L++V + SVG+D +DL + + +G+ +    +V +ED AD+A+GL++ +LR++ 
Sbjct: 66  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 125

Query: 133 AGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSRTKKS 192
             DR+VR G W ++G+F    K SGK +GI+GLG+IG  +AKR E F C ++Y SRT K 
Sbjct: 126 ECDRYVRSGKW-KQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKP 185

Query: 193 MAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAIIDEK 252
              Y YY  V +LA N + L++ C LTE+T H+++++VM ALG  GV++NIGRG  +DE+
Sbjct: 186 DVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQ 245

Query: 253 EMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLVVDNL 312
           E+I+ L +G +GGA LDVFE EP +PEELF L+NVVL PHV   T E+   ++ LVV NL
Sbjct: 246 ELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNL 305

Query: 313 EAFFSNKPLVSPFV 327
           EA FS K L++P V
Sbjct: 306 EAHFSGKSLLTPVV 313

BLAST of Lsi05G019860 vs. ExPASy Swiss-Prot
Match: Q5FTU6 (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 103/266 (38.72%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 61  IRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMA 120
           I  GG + + S I+D LP+L+++  + VG D ++L E RRR + +A   N  ++D ADMA
Sbjct: 46  ITTGGGSGVPSEIMDALPNLEVISVNGVGTDRINLDEARRRNIGVAITQNTLTDDVADMA 105

Query: 121 VGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFG 180
           V L++ V+R +   D FVR G WP     P G  L+ K++GI G G IG  +AKR+  FG
Sbjct: 106 VALMMAVMRSIVTNDAFVRAGKWP-SATAPLGRSLTRKKVGIAGFGHIGQAIAKRVSAFG 165

Query: 181 CKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVI 240
            +V+Y +   +  +   +  ++  LA  C+ LI+  +    + ++I+++ + ALGKDG +
Sbjct: 166 MEVAYFNSHARPESTCHFEPDLKALATWCDVLILAVSGGPRSANMIDRDTLDALGKDGFL 225

Query: 241 VNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYES 300
           VNI RG ++DE  ++  L +  I GAGLDVF+NEP I     +L N VL  H A  T E+
Sbjct: 226 VNIARGTVVDEAALLSALQEKRIAGAGLDVFQNEPNINPAFLSLPNTVLQAHQASATVET 285

Query: 301 SMELSKLVVDNLEAFFSNKPLVSPFV 327
              ++ LVVDNL A+F++K L++P +
Sbjct: 286 RTTMANLVVDNLIAYFTDKTLLTPVI 310

BLAST of Lsi05G019860 vs. ExPASy TrEMBL
Match: A0A5A7TZG5 (Glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004210 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.1e-159
Identity = 291/319 (91.22%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 9   ELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQ 68
           EL QVLVLGPPSIFPYLESQF NRF FLKPWL  L LTQFLTS+AQSTQ LLIRGGGSTQ
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 69  LTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVL 128
           LTSAI+DCLPSLKLVVTSSVGVDHLDLPELRRRGVAIA AGN+FSEDAADMAVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 129 RKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSR 188
           RKVSAGDRFVRQGLW +KGDFPPGLKLSGKRIGI+GLGKIG EVAKRLEGFGCKVSYNSR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGILGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 189 TKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAI 248
           TKKSMA YSYYSNVYELAAN EALIICCALT+ETYHLINKEVM ALGKDGVIVN+GRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYHLINKEVMQALGKDGVIVNVGRGLI 241

Query: 249 IDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLV 308
           IDEKEMIRCLIQGEIGGAGLDVFENEP +PEELFNLDNVVLSPH AVMTYES +ELSKLV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 309 VDNLEAFFSNKPLVSPFVD 328
           V+NLEAFFSNKPLVSP VD
Sbjct: 302 VNNLEAFFSNKPLVSPVVD 320

BLAST of Lsi05G019860 vs. ExPASy TrEMBL
Match: A0A0A0L7K6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124910 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.9e-159
Identity = 290/332 (87.35%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLL 60
           M AE+QAKEL Q+L+LGPPSIFPYLESQFSNRF FLKPWL  L LTQFLTS+AQSTQ LL
Sbjct: 1   MAAEEQAKELPQILILGPPSIFPYLESQFSNRFLFLKPWLYNLPLTQFLTSYAQSTQALL 60

Query: 61  IRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADM- 120
           IRGGG+TQLTS I+DCLPSLKLVVTSSVGVDHLD PELRRRGVAIA AGN+FSED ADM 
Sbjct: 61  IRGGGNTQLTSTIIDCLPSLKLVVTSSVGVDHLDFPELRRRGVAIANAGNLFSEDTADMA 120

Query: 121 ----AVGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKR 180
               AVGLLIDVLRK+SAGDRFVRQGLW +K DFPPGLKLSGKRIGIVGLGKIG EVAKR
Sbjct: 121 GGGVAVGLLIDVLRKISAGDRFVRQGLWSKKEDFPPGLKLSGKRIGIVGLGKIGSEVAKR 180

Query: 181 LEGFGCKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALG 240
           LEGFGCK+SYNSRTKKSMA YSYY NVYELAAN EALIICCALT+ETYHLINKEVMLALG
Sbjct: 181 LEGFGCKISYNSRTKKSMAPYSYYPNVYELAANTEALIICCALTKETYHLINKEVMLALG 240

Query: 241 KDGVIVNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAV 300
           KDGVIVNIGRG IIDEKEMIRCL QGEIGGAGLDVFENEP +PEELFNLDNVVLSPH AV
Sbjct: 241 KDGVIVNIGRGLIIDEKEMIRCLTQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHAAV 300

Query: 301 MTYESSMELSKLVVDNLEAFFSNKPLVSPFVD 328
           MTYES +ELSKLVV+NLEAFFSNKPLVSP VD
Sbjct: 301 MTYESKVELSKLVVNNLEAFFSNKPLVSPVVD 332

BLAST of Lsi05G019860 vs. ExPASy TrEMBL
Match: A0A1S3AW81 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103483325 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.9e-158
Identity = 290/319 (90.91%), Postives = 299/319 (93.73%), Query Frame = 0

Query: 9   ELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQ 68
           EL QVLVLGPPSIFPYLESQF NRF FLKPWL  L LTQFLTS+AQSTQ LLIRGGGSTQ
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 69  LTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVL 128
           LTSAI+DCLPSLKLVVTSSVGVDHLDLPELRRRGVAIA AGN+FSEDAADMAVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 129 RKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSR 188
           RKVSAGDRFVRQGLW +KGDFPPGLKLSGKRIGIVGLGKIG EVAKRLEGFGCKVSYNSR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 189 TKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAI 248
           TKKSMA YSYYSNVYELAAN EALIICCALT+ETY LINKEVM ALGKDGVIVN+GRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYRLINKEVMQALGKDGVIVNVGRGLI 241

Query: 249 IDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLV 308
           IDEKEMIRCLIQGEIGGAGLDVFENEP +PEELFNLDNVVLSPH AVMTYES +ELSKLV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 309 VDNLEAFFSNKPLVSPFVD 328
           V+NLEAFFSN PLVSP VD
Sbjct: 302 VNNLEAFFSNTPLVSPVVD 320

BLAST of Lsi05G019860 vs. ExPASy TrEMBL
Match: A0A6J1EEZ0 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=LOC111431925 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.9e-153
Identity = 278/327 (85.02%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLL 60
           M  EDQA EL QVLVLGPP +FPYLESQF NRFHFLKPWLS L LTQFL S+AQS   LL
Sbjct: 1   MGPEDQANELPQVLVLGPPPVFPYLESQFPNRFHFLKPWLSNLPLTQFLISYAQSATALL 60

Query: 61  IRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMA 120
           IRGGG+ QLTSAILDCLPSL+LVVT+SVGVDHLDLPELRRRG+AIAYAGNVFSED ADMA
Sbjct: 61  IRGGGTVQLTSAILDCLPSLQLVVTASVGVDHLDLPELRRRGLAIAYAGNVFSEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFG 180
           +GLLIDV+RKVSAGDRFVRQGL   KGDFP GLKLS KRIGIVGLG+IG EVAKRLEGFG
Sbjct: 121 IGLLIDVIRKVSAGDRFVRQGLRSTKGDFPLGLKLSSKRIGIVGLGQIGSEVAKRLEGFG 180

Query: 181 CKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVI 240
           CK+SY SR KKSMA YSYYSNVYELAANCE LIICCALTEET+H+IN+EVM+ALGKDGVI
Sbjct: 181 CKISYYSRIKKSMAPYSYYSNVYELAANCEVLIICCALTEETHHIINREVMVALGKDGVI 240

Query: 241 VNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYES 300
           VNIGRGAII+EKEMIRCLI+GEIGGAGLDVFENEP+IPEELF+LDNVVL+PHVAV TYES
Sbjct: 241 VNIGRGAIINEKEMIRCLIEGEIGGAGLDVFENEPRIPEELFHLDNVVLTPHVAVGTYES 300

Query: 301 SMELSKLVVDNLEAFFSNKPLVSPFVD 328
            MELSKL+V+NLE FFSNK LVSPFVD
Sbjct: 301 RMELSKLMVENLETFFSNKSLVSPFVD 327

BLAST of Lsi05G019860 vs. ExPASy TrEMBL
Match: A0A6J1D1V9 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111016253 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.3e-142
Identity = 259/324 (79.94%), Postives = 288/324 (88.89%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLL 60
           M  E Q ++L QVLVLGPP++F  LESQF NRFHFLKPWLSKL L QFLTS+AQ  Q L+
Sbjct: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60

Query: 61  IRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMA 120
           I  GGS  LTSAILDCLPSLKLV T+S GVDHLDLPELRRRGVA+AYAGN+FSED ADMA
Sbjct: 61  I-PGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFG 180
           VGLLIDVLRKVSAGDRF+RQGLW  K +FP GLKLSGKRIGIVGLG+IG EVAKRLEGFG
Sbjct: 121 VGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFG 180

Query: 181 CKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVI 240
           C++SYNSRTKK +  YSYYSNV+ELAA CEALIICC LTEET H+IN+EVM+ALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVI 240

Query: 241 VNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYES 300
           +NIGRGAI+DEKEMIRCL+QGEI GAGLDVFENEP+IPE+LF LDNVVLSPHVAV T+E+
Sbjct: 241 INIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHET 300

Query: 301 SMELSKLVVDNLEAFFSNKPLVSP 325
            + LSKL+VDNLEAFFSN+PLVSP
Sbjct: 301 FLRLSKLMVDNLEAFFSNRPLVSP 323

BLAST of Lsi05G019860 vs. NCBI nr
Match: XP_004133897.3 (glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus] >KAE8650264.1 hypothetical protein Csa_010994 [Cucumis sativus])

HSP 1 Score: 575.9 bits (1483), Expect = 2.3e-160
Identity = 290/327 (88.69%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLL 60
           M AE+QAKEL Q+L+LGPPSIFPYLESQFSNRF FLKPWL  L LTQFLTS+AQSTQ LL
Sbjct: 1   MAAEEQAKELPQILILGPPSIFPYLESQFSNRFLFLKPWLYNLPLTQFLTSYAQSTQALL 60

Query: 61  IRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMA 120
           IRGGG+TQLTS I+DCLPSLKLVVTSSVGVDHLD PELRRRGVAIA AGN+FSED ADMA
Sbjct: 61  IRGGGNTQLTSTIIDCLPSLKLVVTSSVGVDHLDFPELRRRGVAIANAGNLFSEDTADMA 120

Query: 121 VGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFG 180
           VGLLIDVLRK+SAGDRFVRQGLW +K DFPPGLKLSGKRIGIVGLGKIG EVAKRLEGFG
Sbjct: 121 VGLLIDVLRKISAGDRFVRQGLWSKKEDFPPGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVI 240
           CK+SYNSRTKKSMA YSYY NVYELAAN EALIICCALT+ETYHLINKEVMLALGKDGVI
Sbjct: 181 CKISYNSRTKKSMAPYSYYPNVYELAANTEALIICCALTKETYHLINKEVMLALGKDGVI 240

Query: 241 VNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYES 300
           VNIGRG IIDEKEMIRCL QGEIGGAGLDVFENEP +PEELFNLDNVVLSPH AVMTYES
Sbjct: 241 VNIGRGLIIDEKEMIRCLTQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHAAVMTYES 300

Query: 301 SMELSKLVVDNLEAFFSNKPLVSPFVD 328
            +ELSKLVV+NLEAFFSNKPLVSP VD
Sbjct: 301 KVELSKLVVNNLEAFFSNKPLVSPVVD 327

BLAST of Lsi05G019860 vs. NCBI nr
Match: KAA0049013.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa] >TYK17551.1 glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa])

HSP 1 Score: 571.6 bits (1472), Expect = 4.3e-159
Identity = 291/319 (91.22%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 9   ELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQ 68
           EL QVLVLGPPSIFPYLESQF NRF FLKPWL  L LTQFLTS+AQSTQ LLIRGGGSTQ
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 69  LTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVL 128
           LTSAI+DCLPSLKLVVTSSVGVDHLDLPELRRRGVAIA AGN+FSEDAADMAVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 129 RKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSR 188
           RKVSAGDRFVRQGLW +KGDFPPGLKLSGKRIGI+GLGKIG EVAKRLEGFGCKVSYNSR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGILGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 189 TKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAI 248
           TKKSMA YSYYSNVYELAAN EALIICCALT+ETYHLINKEVM ALGKDGVIVN+GRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYHLINKEVMQALGKDGVIVNVGRGLI 241

Query: 249 IDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLV 308
           IDEKEMIRCLIQGEIGGAGLDVFENEP +PEELFNLDNVVLSPH AVMTYES +ELSKLV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 309 VDNLEAFFSNKPLVSPFVD 328
           V+NLEAFFSNKPLVSP VD
Sbjct: 302 VNNLEAFFSNKPLVSPVVD 320

BLAST of Lsi05G019860 vs. NCBI nr
Match: XP_008438123.1 (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 567.4 bits (1461), Expect = 8.1e-158
Identity = 290/319 (90.91%), Postives = 299/319 (93.73%), Query Frame = 0

Query: 9   ELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQ 68
           EL QVLVLGPPSIFPYLESQF NRF FLKPWL  L LTQFLTS+AQSTQ LLIRGGGSTQ
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 69  LTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVL 128
           LTSAI+DCLPSLKLVVTSSVGVDHLDLPELRRRGVAIA AGN+FSEDAADMAVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 129 RKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSR 188
           RKVSAGDRFVRQGLW +KGDFPPGLKLSGKRIGIVGLGKIG EVAKRLEGFGCKVSYNSR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 189 TKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAI 248
           TKKSMA YSYYSNVYELAAN EALIICCALT+ETY LINKEVM ALGKDGVIVN+GRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYRLINKEVMQALGKDGVIVNVGRGLI 241

Query: 249 IDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLV 308
           IDEKEMIRCLIQGEIGGAGLDVFENEP +PEELFNLDNVVLSPH AVMTYES +ELSKLV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 309 VDNLEAFFSNKPLVSPFVD 328
           V+NLEAFFSN PLVSP VD
Sbjct: 302 VNNLEAFFSNTPLVSPVVD 320

BLAST of Lsi05G019860 vs. NCBI nr
Match: XP_038902451.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Benincasa hispida])

HSP 1 Score: 560.8 bits (1444), Expect = 7.5e-156
Identity = 289/328 (88.11%), Postives = 299/328 (91.16%), Query Frame = 0

Query: 1   MTAEDQAK-ELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTL 60
           M  EDQAK EL QVLVLGPP IFPYLESQF+NRFHFLKPWLS LSLTQFLTS+ QSTQ L
Sbjct: 1   MATEDQAKDELPQVLVLGPPLIFPYLESQFTNRFHFLKPWLSNLSLTQFLTSYTQSTQAL 60

Query: 61  LIRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADM 120
           LIRGGG+ QLT+AILDCLPSLKLVVTSSVGVDHL LPEL RRGVAIA+A NVFSEDAADM
Sbjct: 61  LIRGGGTAQLTTAILDCLPSLKLVVTSSVGVDHLYLPELHRRGVAIAHARNVFSEDAADM 120

Query: 121 AVGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGF 180
           AVGLLIDVLRKVSAGDRFVRQGLW  KG FP GLKLSGKRIGIVGLGKIG EVAKRLEGF
Sbjct: 121 AVGLLIDVLRKVSAGDRFVRQGLWLTKGHFPSGLKLSGKRIGIVGLGKIGSEVAKRLEGF 180

Query: 181 GCKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGV 240
           GCK+SYNSR KKS A YSYYSNVYELAANCE LIICCALTEETYH+INKEVM+ALGKDGV
Sbjct: 181 GCKISYNSRIKKSKAPYSYYSNVYELAANCEVLIICCALTEETYHIINKEVMVALGKDGV 240

Query: 241 IVNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYE 300
           IVNIGRGAIIDEKEMIR LIQGEIGGAGLDVFENEP I EE  NLDNVVLSPH A MTYE
Sbjct: 241 IVNIGRGAIIDEKEMIRRLIQGEIGGAGLDVFENEPVIAEEFLNLDNVVLSPHAAAMTYE 300

Query: 301 SSMELSKLVVDNLEAFFSNKPLVSPFVD 328
           SSME+ +LVVDNLEAFFSNKPLVSPFVD
Sbjct: 301 SSMEVCRLVVDNLEAFFSNKPLVSPFVD 328

BLAST of Lsi05G019860 vs. NCBI nr
Match: XP_023526607.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.8 bits (1436), Expect = 6.4e-155
Identity = 281/327 (85.93%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLL 60
           M  EDQA +L QVLVLGPP +FPYLESQF NRFHFLKPWLS L LTQFL S+AQS   LL
Sbjct: 1   MGPEDQANQLPQVLVLGPPPVFPYLESQFPNRFHFLKPWLSHLPLTQFLISYAQSATALL 60

Query: 61  IRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMA 120
           IRGGG+ QLTSAILDCLPSL+LVVT+SVGVDHLDLPELRRRG+AIAYAGNVF+ED ADMA
Sbjct: 61  IRGGGTVQLTSAILDCLPSLQLVVTASVGVDHLDLPELRRRGLAIAYAGNVFTEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFG 180
           +GLLIDVLRKVSAGDRFVRQGLW  KGDFP GLKLS KRIGIVGLGKIG EVAKRLEGFG
Sbjct: 121 IGLLIDVLRKVSAGDRFVRQGLWSTKGDFPLGLKLSSKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CKVSYNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVI 240
           CKVSY SR KKSMA YSYYSNVYELAANCE LIICCALTEET+H+INKEVM+ALGKDGVI
Sbjct: 181 CKVSYYSRIKKSMAPYSYYSNVYELAANCEVLIICCALTEETHHIINKEVMVALGKDGVI 240

Query: 241 VNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYES 300
           VNIGRGAII+EKEMIRCLI+GEIGGAGLDVFENEP+IPEELF+LDNVVL+PHVAV TYES
Sbjct: 241 VNIGRGAIINEKEMIRCLIEGEIGGAGLDVFENEPRIPEELFHLDNVVLTPHVAVGTYES 300

Query: 301 SMELSKLVVDNLEAFFSNKPLVSPFVD 328
            MELSKL+V+NLE FFSNK LVSPFVD
Sbjct: 301 RMELSKLMVENLETFFSNKSLVSPFVD 327

BLAST of Lsi05G019860 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.2e-95
Identity = 181/327 (55.35%), Postives = 234/327 (71.56%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQF--SNRFHFLKPWLSKLSLTQFLTSFAQSTQT 60
           M      ++L +VL++  P     L   F  S +F  LK + S L L +FL   + S   
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  LLIRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAAD 120
           ++     +  +T+ ++  LP+L+LVVT+S GVDH+DL E RRRG+++A AG+ FSED AD
Sbjct: 72  II--APVAAPVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 131

Query: 121 MAVGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEG 180
            AVGLLIDV R++SA +RFV+Q  WP KGD+P G KL  KRIGIVGLG IG +VA RL+ 
Sbjct: 132 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDA 191

Query: 181 FGCKVSYNSRTKKSM-AQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKD 240
           FGC++SY+SR +K     Y YY ++ E+AAN +ALIICC L E+T  LINK+V+ ALGK 
Sbjct: 192 FGCQISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKR 251

Query: 241 GVIVNIGRGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMT 300
           GVIVN+ RGAIIDE+EM+RCL +GEIGGAGLDVFE+EP +P+ELF LDNVV SPH A MT
Sbjct: 252 GVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMT 311

Query: 301 YESSMELSKLVVDNLEAFFSNKPLVSP 325
            E   EL K+VV N+EAFFSNKPL++P
Sbjct: 312 LEGLEELGKVVVGNIEAFFSNKPLLTP 336

BLAST of Lsi05G019860 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.1e-91
Identity = 168/320 (52.50%), Postives = 228/320 (71.25%), Query Frame = 0

Query: 6   QAKELSQVLVLGPPSIFPYLESQFSNRFH-FLKPWLSKLSLTQFLTSFAQSTQTLLIRGG 65
           ++ E   VL+  PPS+  +++   +  F   +    S  SL  F    A S +  +I   
Sbjct: 3   ESSEPPVVLLHRPPSL-TFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI--S 62

Query: 66  GSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLL 125
           G   +T  +L  LPSL+++V +SVG+DH+DL   +RRG+ I  AGN FS+D AD AVGLL
Sbjct: 63  GRLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLL 122

Query: 126 IDVLRKVSAGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVS 185
           I VLR++ A DR+VR G W + GDF  G K+SGKR+GIVGLG IG  VAKRLE FGC +S
Sbjct: 123 ISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVIS 182

Query: 186 YNSRTKKSMAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIG 245
           YNSR++K  + Y YYS++  LA N + L++CC+LT+ET+H++N+EVM  LGKDGV++N+G
Sbjct: 183 YNSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVG 242

Query: 246 RGAIIDEKEMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMEL 305
           RG +IDEKEM++CL+ G IGGAGLDVFENEP +P+ELF LDNVVLSPH AV T  S   +
Sbjct: 243 RGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNV 302

Query: 306 SKLVVDNLEAFFSNKPLVSP 325
           +++ + NL+AFFSN+PL+SP
Sbjct: 303 AQIALANLKAFFSNRPLLSP 319

BLAST of Lsi05G019860 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.8e-70
Identity = 137/314 (43.63%), Postives = 206/314 (65.61%), Query Frame = 0

Query: 13  VLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQLTSA 72
           VL++ P S   YLE++   RF+ L+ W S    +  L +   S + ++  G  S    + 
Sbjct: 6   VLMMCPMS--SYLENELEKRFNLLRFWTSP-EKSVLLETHRNSIRAVV--GNASAGADAQ 65

Query: 73  ILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVLRKVS 132
           ++  LP+L++V + SVG+D +DL + + +G+ +    +V +ED AD+A+GL++ +LR++ 
Sbjct: 66  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 125

Query: 133 AGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSRTKKS 192
             DR+VR G W ++G+F    K SGK +GI+GLG+IG  +AKR E F C ++Y SRT K 
Sbjct: 126 ECDRYVRSGKW-KQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKP 185

Query: 193 MAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAIIDEK 252
              Y YY  V +LA N + L++ C LTE+T H+++++VM ALG  GV++NIGRG  +DE+
Sbjct: 186 DVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQ 245

Query: 253 EMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLVVDNL 312
           E+I+ L +G +GGA LDVFE EP +PEELF L+NVVL PHV   T E+   ++ LVV NL
Sbjct: 246 ELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNL 305

Query: 313 EAFFSNKPLVSPFV 327
           EA FS K L++P V
Sbjct: 306 EAHFSGKSLLTPVV 313

BLAST of Lsi05G019860 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 235.0 bits (598), Expect = 8.8e-62
Identity = 127/314 (40.45%), Postives = 192/314 (61.15%), Query Frame = 0

Query: 13  VLVLGPPSIFPYLESQFSNRFHFLKPWLSKLSLTQFLTSFAQSTQTLLIRGGGSTQLTSA 72
           VL++ P S   YLE++   RF+ L+ W S    +  L +   S + ++  G  S    + 
Sbjct: 6   VLMMCPMS--SYLENELEKRFNLLRFWTSP-EKSVLLETHRNSIRAVV--GNASAGADAQ 65

Query: 73  ILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAADMAVGLLIDVLRKVS 132
           ++  LP+L++V + SVG+D +DL + + +G+ +    +V +ED AD+A+GL++ +LR++ 
Sbjct: 66  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 125

Query: 133 AGDRFVRQGLWPRKGDFPPGLKLSGKRIGIVGLGKIGYEVAKRLEGFGCKVSYNSRTKKS 192
             DR+VR G W +                    G+IG  +AKR E F C ++Y SRT K 
Sbjct: 126 ECDRYVRSGKWKQ--------------------GRIGTAIAKRAEAFSCPINYYSRTIKP 185

Query: 193 MAQYSYYSNVYELAANCEALIICCALTEETYHLINKEVMLALGKDGVIVNIGRGAIIDEK 252
              Y YY  V +LA N + L++ C LTE+T H+++++VM ALG  GV++NIGRG  +DE+
Sbjct: 186 DVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQ 245

Query: 253 EMIRCLIQGEIGGAGLDVFENEPKIPEELFNLDNVVLSPHVAVMTYESSMELSKLVVDNL 312
           E+I+ L +G +GGA LDVFE EP +PEELF L+NVVL PHV   T E+   ++ LVV NL
Sbjct: 246 ELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNL 294

Query: 313 EAFFSNKPLVSPFV 327
           EA FS K L++P V
Sbjct: 306 EAHFSGKSLLTPVV 294

BLAST of Lsi05G019860 vs. TAIR 10
Match: AT2G45630.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 127.5 bits (319), Expect = 2.0e-29
Identity = 69/157 (43.95%), Postives = 99/157 (63.06%), Query Frame = 0

Query: 1   MTAEDQAKELSQVLVLGPPSIFPYLESQF--SNRFHFLKPWLSKLSLTQFLTSFAQSTQT 60
           M      ++L +VL++  P     L   F  S +F  LK + S L L +FL   + S   
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  LLIRGGGSTQLTSAILDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIAYAGNVFSEDAAD 120
           ++     +  +T+ ++  LP+L+LVVT+S GVDH+DL E RRRG+++A AG+ FSED AD
Sbjct: 72  II--APVAAPVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 131

Query: 121 MAVGLLIDVLRKVSAGDRFVRQGLWPRKGDFPPGLKL 156
            AVGLLIDV R++SA +RFV+Q  WP KGD+P G K+
Sbjct: 132 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKV 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LE331.5e-9052.50Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR... [more]
A5CAL14.4e-7445.07Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN... [more]
Q65CJ72.2e-7345.54Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ... [more]
Q9CA902.5e-6943.63Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q5FTU61.2e-5038.722-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GO... [more]
Match NameE-valueIdentityDescription
A0A5A7TZG52.1e-15991.22Glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0L7K67.9e-15987.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124910 PE=3 SV=1[more]
A0A1S3AW813.9e-15890.91glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1EEZ02.9e-15385.02glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1D1V91.3e-14279.94glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
XP_004133897.32.3e-16088.69glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus] >KAE8650264.1 hypoth... [more]
KAA0049013.14.3e-15991.22glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa] >TYK17... [more]
XP_008438123.18.1e-15890.91PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo][more]
XP_038902451.17.5e-15688.11glyoxylate/hydroxypyruvate reductase HPR3-like [Benincasa hispida][more]
XP_023526607.16.4e-15585.93glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G45630.21.2e-9555.35D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G12550.11.1e-9152.50D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.11.8e-7043.63D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.28.8e-6240.45D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT2G45630.12.0e-2943.95D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 121..294
e-value: 1.3E-50
score: 171.2
NoneNo IPR availableGENE3D3.40.50.720coord: 113..297
e-value: 3.7E-101
score: 339.7
NoneNo IPR availableGENE3D3.40.50.720coord: 28..320
e-value: 3.7E-101
score: 339.7
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 8..325
NoneNo IPR availablePANTHERPTHR10996:SF179D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN-RELATEDcoord: 8..325
NoneNo IPR availableCDDcd12156HPPRcoord: 13..317
e-value: 5.96247E-143
score: 403.773
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 13..148
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 52..321
e-value: 7.0E-16
score: 58.4
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 113..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G019860.1Lsi05G019860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0030267 glyoxylate reductase (NADP+) activity
molecular_function GO:0016618 hydroxypyruvate reductase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor