Lsi05G019850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G019850
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionglyoxylate/hydroxypyruvate reductase HPR3-like
Locationchr05: 26955047 .. 26956625 (+)
RNA-Seq ExpressionLsi05G019850
SyntenyLsi05G019850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTAATGGCTCCTCTGTGATGCATTTAAGCTAAAAAAAAAAATTGTTTTGATGGCACATTCCAACCATCTAATAATTTAACTAGTTCCCTTTCTTCATCCTTTCATTCGAATTTTATGTAAGGGATCCCAAGAAGAGCATAAATGAATGTCATTATCTTCTCACGTATTCCAAGAAAAGTCCATTTTTTTCATGAAGAAACTTGTGTTGTTTTAGTCCATCTCTGATCCCTCCCTCAATCAGCTTATTTTTACAAGCAAGTATAAATTAGAATGGCAGTCGAAAGACAATCCGAGGAGCTTCCTCAAGTTTTGGTTCTAAGTCCTCCTACGCTATTCACTTCTCTGGAATCTCAATTTCCAAACAGATTCCATTTCCTCAAACCGTGGAATTCCAAACTCCCACTTCTCCAATTCCTCATCTCTAATGCACAATCCGCCCTAGCTTGTCTTGTCATTCCAGGTGACCGTCTCGCACTGTCCTCCGCCATTCTCGACTGCCTCCCCTCCCTCAAACTCCTTGTCACTACTAGCGCCGGTGTCGATCACTTGCACGTGGCCGAGCTACGGCGGCGCGGTGTAGCCATAGCCTATGCAGGGAACTTGTTCTCTCAAGATGTGGCCGATATGGCTGTGGGGTTATTAATCGACGTTCTTAGGAATGTTTCGGCGGGAGATCGGTTCGTGAGGCGAGGGCTTTGGCCTACGCAAGGGGATTTTCCTCTCGGATTAAAGGTCCACTCTGGTTTCATCTAATTGCGAATTCTTGTTTAGGTTTCTAATTAGTTAGGGCAAGGTTTCAGAGTGATAAAATCTCAGGTTCAAAATCATGTCAAAACGCGGTTTTGATCATTCAAAATCACCTTTGATGGTTGGTAAATCCTGTTCAAAGGTGTATAACCACACAATTTTGGATGATTAAATGCATTTTTTGAAGTGATTCTGAATATGAGCTGAGTTCATCTCAAACATGCCCTTTATCTCTCGTGGAAGAAGTGTAGTTGAATTTGAATGTTTTTTTTAGATCTATGAATTTCAAGGTTGAAAAATTTTCACAGTTGAGCGGCAAGCGAATTGGGATTGTTGGATTGGGGAAAATAGGCTCTGAAGTTGCCAAAAGACTGGAGGGTTTTGGGTGCAGAATCTCATACAACTCAAGGACCAAAAAACCATTAGTTTTATACTCCCATTATTCCAATGTACATGAACTTGCAACCAACTGTGATGCCCTTATCATTTGTTGTGGGTTAACAGAAGAAACCCACCATTTGATCAACAGGGAGGTGATGGTTGCATTGGGAAAAGATGGAGTGATAATCAACGTCGGTCGAGGGGCGATCATCGACGAGAAGGCGATGATTGAATGTTTGATTCAAGGGGAGATTAGAGGGGCTGGGCTGGATGTTTTTGAGGATGAACCTGAAATTCCTAAACAGTTGTTTAATCTTGATAATGTCGTGTTGTCGCCGCATGTTGCTGTTACAACAACTGAATCTACGGTGGGATTGACTGAGTTGGTGGTGGAGAATTTGGAGGCATTCTTCTCAAACAAACCTTTGGTGTCTCCCTTTCTGGATTAG

mRNA sequence

TTTTAATGGCTCCTCTGTGATGCATTTAAGCTAAAAAAAAAAATTGTTTTGATGGCACATTCCAACCATCTAATAATTTAACTAGTTCCCTTTCTTCATCCTTTCATTCGAATTTTATGTAAGGGATCCCAAGAAGAGCATAAATGAATGTCATTATCTTCTCACGTATTCCAAGAAAAGTCCATTTTTTTCATGAAGAAACTTGTGTTGTTTTAGTCCATCTCTGATCCCTCCCTCAATCAGCTTATTTTTACAAGCAAGTATAAATTAGAATGGCAGTCGAAAGACAATCCGAGGAGCTTCCTCAAGTTTTGGTTCTAAGTCCTCCTACGCTATTCACTTCTCTGGAATCTCAATTTCCAAACAGATTCCATTTCCTCAAACCGTGGAATTCCAAACTCCCACTTCTCCAATTCCTCATCTCTAATGCACAATCCGCCCTAGCTTGTCTTGTCATTCCAGGTGACCGTCTCGCACTGTCCTCCGCCATTCTCGACTGCCTCCCCTCCCTCAAACTCCTTGTCACTACTAGCGCCGGTGTCGATCACTTGCACGTGGCCGAGCTACGGCGGCGCGGTGTAGCCATAGCCTATGCAGGGAACTTGTTCTCTCAAGATGTGGCCGATATGGCTGTGGGGTTATTAATCGACGTTCTTAGGAATGTTTCGGCGGGAGATCGGTTCGTGAGGCGAGGGCTTTGGCCTACGCAAGGGGATTTTCCTCTCGGATTAAAGTTGAGCGGCAAGCGAATTGGGATTGTTGGATTGGGGAAAATAGGCTCTGAAGTTGCCAAAAGACTGGAGGGTTTTGGGTGCAGAATCTCATACAACTCAAGGACCAAAAAACCATTAGTTTTATACTCCCATTATTCCAATGTACATGAACTTGCAACCAACTGTGATGCCCTTATCATTTGTTGTGGGTTAACAGAAGAAACCCACCATTTGATCAACAGGGAGGTGATGGTTGCATTGGGAAAAGATGGAGTGATAATCAACGTCGGTCGAGGGGCGATCATCGACGAGAAGGCGATGATTGAATGTTTGATTCAAGGGGAGATTAGAGGGGCTGGGCTGGATGTTTTTGAGGATGAACCTGAAATTCCTAAACAGTTGTTTAATCTTGATAATGTCGTGTTGTCGCCGCATGTTGCTGTTACAACAACTGAATCTACGGTGGGATTGACTGAGTTGGTGGTGGAGAATTTGGAGGCATTCTTCTCAAACAAACCTTTGGTGTCTCCCTTTCTGGATTAG

Coding sequence (CDS)

ATGGCAGTCGAAAGACAATCCGAGGAGCTTCCTCAAGTTTTGGTTCTAAGTCCTCCTACGCTATTCACTTCTCTGGAATCTCAATTTCCAAACAGATTCCATTTCCTCAAACCGTGGAATTCCAAACTCCCACTTCTCCAATTCCTCATCTCTAATGCACAATCCGCCCTAGCTTGTCTTGTCATTCCAGGTGACCGTCTCGCACTGTCCTCCGCCATTCTCGACTGCCTCCCCTCCCTCAAACTCCTTGTCACTACTAGCGCCGGTGTCGATCACTTGCACGTGGCCGAGCTACGGCGGCGCGGTGTAGCCATAGCCTATGCAGGGAACTTGTTCTCTCAAGATGTGGCCGATATGGCTGTGGGGTTATTAATCGACGTTCTTAGGAATGTTTCGGCGGGAGATCGGTTCGTGAGGCGAGGGCTTTGGCCTACGCAAGGGGATTTTCCTCTCGGATTAAAGTTGAGCGGCAAGCGAATTGGGATTGTTGGATTGGGGAAAATAGGCTCTGAAGTTGCCAAAAGACTGGAGGGTTTTGGGTGCAGAATCTCATACAACTCAAGGACCAAAAAACCATTAGTTTTATACTCCCATTATTCCAATGTACATGAACTTGCAACCAACTGTGATGCCCTTATCATTTGTTGTGGGTTAACAGAAGAAACCCACCATTTGATCAACAGGGAGGTGATGGTTGCATTGGGAAAAGATGGAGTGATAATCAACGTCGGTCGAGGGGCGATCATCGACGAGAAGGCGATGATTGAATGTTTGATTCAAGGGGAGATTAGAGGGGCTGGGCTGGATGTTTTTGAGGATGAACCTGAAATTCCTAAACAGTTGTTTAATCTTGATAATGTCGTGTTGTCGCCGCATGTTGCTGTTACAACAACTGAATCTACGGTGGGATTGACTGAGTTGGTGGTGGAGAATTTGGAGGCATTCTTCTCAAACAAACCTTTGGTGTCTCCCTTTCTGGATTAG

Protein sequence

MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTELVVENLEAFFSNKPLVSPFLD
Homology
BLAST of Lsi05G019850 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.6e-90
Identity = 175/319 (54.86%), Postives = 221/319 (69.28%), Query Frame = 0

Query: 6   QSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGD 65
           +S E P VL+  PP+L T ++      F  L    S    L        S+    VI G 
Sbjct: 3   ESSEPPVVLLHRPPSL-TFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISG- 62

Query: 66  RLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLI 125
           RL ++  +L  LPSL++LV TS G+DH+ +A  +RRG+ I  AGN FS DVAD AVGLLI
Sbjct: 63  RLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLI 122

Query: 126 DVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISY 185
            VLR + A DR+VR G W   GDF LG K+SGKR+GIVGLG IGS VAKRLE FGC ISY
Sbjct: 123 SVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISY 182

Query: 186 NSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGR 245
           NSR++K    Y +YS++  LA N D L++CC LT+ETHH++NREVM  LGKDGV+INVGR
Sbjct: 183 NSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGR 242

Query: 246 GAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLT 305
           G +IDEK M++CL+ G I GAGLDVFE+EP +P++LF LDNVVLSPH AV T  S   + 
Sbjct: 243 GKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVA 302

Query: 306 ELVVENLEAFFSNKPLVSP 325
           ++ + NL+AFFSN+PL+SP
Sbjct: 303 QIALANLKAFFSNRPLLSP 319

BLAST of Lsi05G019850 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-70
Identity = 141/312 (45.19%), Postives = 201/312 (64.42%), Query Frame = 0

Query: 13  VLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGDRLALSSA 72
           VL++ P  + T LE +   RF   + W        FL   A+S  A  V+        + 
Sbjct: 6   VLMMCP--MSTYLEQELDKRFKLFRYWTQPAQ-RDFLALQAESIRA--VVGNSNAGADAE 65

Query: 73  ILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVS 132
           ++D LP L+++ + S G+D + + +   +GV +    ++ + DVAD+A+GL++ VLR + 
Sbjct: 66  LIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRIC 125

Query: 133 AGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKP 192
             D++VRRG W   GDF L  K SGKR+GI+GLG+IG  VA+R E F C ISY SR+KKP
Sbjct: 126 ECDKYVRRGAWKF-GDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKP 185

Query: 193 LVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRGAIIDEK 252
              Y++Y +V ELA+N D L++ C LT ET H+INREV+ ALG  GV+IN+GRG  +DE 
Sbjct: 186 NTNYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEP 245

Query: 253 AMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTELVVENL 312
            ++  L++G + GAGLDVFE EPE+P++LF L+NVVL PHV   T E+   + +LVV NL
Sbjct: 246 ELVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNL 305

Query: 313 EAFFSNKPLVSP 325
           EA FS KPL++P
Sbjct: 306 EAHFSGKPLLTP 311

BLAST of Lsi05G019850 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.8e-68
Identity = 126/300 (42.00%), Postives = 194/300 (64.67%), Query Frame = 0

Query: 25  LESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGDRLALSSAILDCLPSLKLLV 84
           LE +   RF   + W+   P    L     +++   V+    +   + +++ LP ++++ 
Sbjct: 16  LEQELDKRFKLFRFWD--FPSANDLFREHSNSIRA-VVGNSFIGADAQMIEALPKMEIVS 75

Query: 85  TTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNVSAGDRFVRRGLWP 144
           + S G+D + +   + +G+ +    ++ ++DVAD+A+ L++  LR +   DR+VR G W 
Sbjct: 76  SFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSGSW- 135

Query: 145 TQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKKPLVLYSHYSNVHE 204
            +GDF L  K +GK +GI+GLG+IGS +AKR EGF C ISY+SRT+KP   Y +Y +V E
Sbjct: 136 KKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPSVVE 195

Query: 205 LATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRGAIIDEKAMIECLIQGEIR 264
           LA+NC  L++ C LT ET H+INREV+ ALG  GV+IN+GRG  +DE  ++  L++G + 
Sbjct: 196 LASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRLG 255

Query: 265 GAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTELVVENLEAFFSNKPLVSP 324
           GAGLDVFE+EP +P++L  +DNVVL PHV   T E+   + +LV+ NLEA F NKPL++P
Sbjct: 256 GAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLTP 311

BLAST of Lsi05G019850 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 6.8e-67
Identity = 133/313 (42.49%), Postives = 208/313 (66.45%), Query Frame = 0

Query: 13  VLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGDRLALSSA 72
           VL++ P + +  LE++   RF+ L+ W S  P    L+   ++++  +V  G+  A + A
Sbjct: 6   VLMMCPMSSY--LENELEKRFNLLRFWTS--PEKSVLLETHRNSIRAVV--GNASAGADA 65

Query: 73  -ILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNV 132
            ++  LP+L+++ + S G+D + + + + +G+ +    ++ ++DVAD+A+GL++ +LR +
Sbjct: 66  QLISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRL 125

Query: 133 SAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKK 192
              DR+VR G W  QG+F L  K SGK +GI+GLG+IG+ +AKR E F C I+Y SRT K
Sbjct: 126 CECDRYVRSGKW-KQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIK 185

Query: 193 PLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRGAIIDE 252
           P V Y +Y  V +LA N D L++ C LTE+T H+++R+VM ALG  GV+IN+GRG  +DE
Sbjct: 186 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 245

Query: 253 KAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTELVVEN 312
           + +I+ L +G + GA LDVFE EP +P++LF L+NVVL PHV   T E+   + +LVV N
Sbjct: 246 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 305

Query: 313 LEAFFSNKPLVSP 325
           LEA FS K L++P
Sbjct: 306 LEAHFSGKSLLTP 311

BLAST of Lsi05G019850 vs. ExPASy Swiss-Prot
Match: Q5FTU6 (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.6e-50
Identity = 112/320 (35.00%), Postives = 177/320 (55.31%), Query Frame = 0

Query: 11  PQVLVLSP--PTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL--VIPGDR 70
           P +L + P  P +   LE  F      L P+ S        + N ++    +  +  G  
Sbjct: 5   PDILTIDPLVPVMKERLEKSFT-----LHPYTS--------LENLKNIAPAIRGITTGGG 64

Query: 71  LALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLID 130
             + S I+D LP+L+++     G D +++ E RRR + +A   N  + DVADMAV L++ 
Sbjct: 65  SGVPSEIMDALPNLEVISVNGVGTDRINLDEARRRNIGVAITQNTLTDDVADMAVALMMA 124

Query: 131 VLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYN 190
           V+R++   D FVR G WP+    PLG  L+ K++GI G G IG  +AKR+  FG  ++Y 
Sbjct: 125 VMRSIVTNDAFVRAGKWPS-ATAPLGRSLTRKKVGIAGFGHIGQAIAKRVSAFGMEVAYF 184

Query: 191 SRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRG 250
           +   +P        ++  LAT CD LI+       + ++I+R+ + ALGKDG ++N+ RG
Sbjct: 185 NSHARPESTCHFEPDLKALATWCDVLILAVSGGPRSANMIDRDTLDALGKDGFLVNIARG 244

Query: 251 AIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTE 310
            ++DE A++  L +  I GAGLDVF++EP I     +L N VL  H A  T E+   +  
Sbjct: 245 TVVDEAALLSALQEKRIAGAGLDVFQNEPNINPAFLSLPNTVLQAHQASATVETRTTMAN 304

Query: 311 LVVENLEAFFSNKPLVSPFL 327
           LVV+NL A+F++K L++P +
Sbjct: 305 LVVDNLIAYFTDKTLLTPVI 310

BLAST of Lsi05G019850 vs. ExPASy TrEMBL
Match: A0A5D3D1Q7 (Glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004200 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.3e-160
Identity = 293/327 (89.60%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAVE  S+EL QVLVLSPP  FTSLESQF NRF FL PWNS LPLLQFLISNAQS  ACL
Sbjct: 1   MAVEIPSDELHQVLVLSPPFEFTSLESQFQNRFQFLNPWNSNLPLLQFLISNAQSVRACL 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PG RLA+SSAILDCLPSL+L+VTTSAGVDHLHVAE+RRRGVAIA+AGNL SQDVADMA
Sbjct: 61  VMPGGRLAVSSAILDCLPSLELVVTTSAGVDHLHVAEIRRRGVAIAFAGNLLSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRN+SAGDRFVR+GLWPTQGDF +GLKLSGKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNISAGDRFVRQGLWPTQGDFSIGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YSHYSNVHELATNCDALIICC LTEET HLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDALIICCCLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIECLIQGEI+GAGLDVFE EPEIPKQLFNLDNVVLSPH A TTTES
Sbjct: 241 INVGRGAIIDEKAMIECLIQGEIKGAGLDVFEHEPEIPKQLFNLDNVVLSPHAAGTTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLTEL  ENLEAFFSNKPLVSPFLD
Sbjct: 301 IVGLTELAFENLEAFFSNKPLVSPFLD 327

BLAST of Lsi05G019850 vs. ExPASy TrEMBL
Match: A0A1S3AW95 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103483323 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.3e-160
Identity = 293/327 (89.60%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAVE  S+EL QVLVLSPP  FTSLESQF NRF FL PWNS LPLLQFLISNAQS  ACL
Sbjct: 1   MAVEIPSDELHQVLVLSPPFEFTSLESQFQNRFQFLNPWNSNLPLLQFLISNAQSVRACL 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PG RLA+SSAILDCLPSL+L+VTTSAGVDHLHVAE+RRRGVAIA+AGNL SQDVADMA
Sbjct: 61  VMPGGRLAVSSAILDCLPSLELVVTTSAGVDHLHVAEIRRRGVAIAFAGNLLSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRN+SAGDRFVR+GLWPTQGDF +GLKLSGKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNISAGDRFVRQGLWPTQGDFSIGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YSHYSNVHELATNCDALIICC LTEET HLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDALIICCCLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIECLIQGEI+GAGLDVFE EPEIPKQLFNLDNVVLSPH A TTTES
Sbjct: 241 INVGRGAIIDEKAMIECLIQGEIKGAGLDVFEHEPEIPKQLFNLDNVVLSPHAAGTTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLTEL  ENLEAFFSNKPLVSPFLD
Sbjct: 301 IVGLTELAFENLEAFFSNKPLVSPFLD 327

BLAST of Lsi05G019850 vs. ExPASy TrEMBL
Match: A0A0A0L4C2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124920 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.3e-157
Identity = 291/327 (88.99%), Postives = 303/327 (92.66%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAV+ QS+EL QVLVLSPP++FTSLESQF NRF FLKPW+S LPLLQFLISNAQS  ACL
Sbjct: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V PGD LA+SSAILDCLPSLK +VT SAGVDHL+VAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR+GLW TQ DF LGLKL+GKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YSHYSNVHELATNCD LIIC  LTEET HLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIE LIQGEI+GAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES
Sbjct: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
             GL EL +ENLEAFFSNKPLVSPFLD
Sbjct: 301 IAGLIELALENLEAFFSNKPLVSPFLD 327

BLAST of Lsi05G019850 vs. ExPASy TrEMBL
Match: A0A6J1FT24 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=LOC111448505 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.9e-152
Identity = 278/327 (85.02%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAV RQ  ELPQVLVLSPP +FTSLESQF  RFH LKPW+SKLPLLQFL S AQS  A L
Sbjct: 1   MAVGRQPNELPQVLVLSPPAIFTSLESQFSIRFHMLKPWDSKLPLLQFLTSYAQSVPAML 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PGDRL ++SAILDCLPSLKL+VTTSAGVDHL VAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VVPGDRLLVNSAILDCLPSLKLVVTTSAGVDHLDVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR GLWPTQ DFPL LKLSGK+IGIVGLGKIG+EVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRLGLWPTQRDFPLALKLSGKQIGIVGLGKIGTEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YS+YSNVHELA NC+ALIICCGLT ET H+I+REVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVSYSYYSNVHELAANCEALIICCGLTAETRHMIDREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAI+DE  +I CLIQGEIRGAGLDVFE++PEIPK+LFNLDNVVLSPHVAV TTES
Sbjct: 241 INVGRGAIVDETELIRCLIQGEIRGAGLDVFENDPEIPKELFNLDNVVLSPHVAVLTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLT+L + NLEAFFSNKPLVSP++D
Sbjct: 301 MVGLTQLALGNLEAFFSNKPLVSPYVD 327

BLAST of Lsi05G019850 vs. ExPASy TrEMBL
Match: A0A6J1IDA0 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita maxima OX=3661 GN=LOC111472755 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.0e-147
Identity = 265/327 (81.04%), Postives = 296/327 (90.52%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAVERQ  ELPQVLVLSPP +FTSLESQF  RFH LKPW+SKLPLLQFL S AQS  A L
Sbjct: 1   MAVERQPNELPQVLVLSPPAIFTSLESQFSIRFHMLKPWDSKLPLLQFLTSYAQSVPAML 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PGDRL ++SAILDCLPSLKL+VTTSAGVDHL VAELRRR VAIAYAGNLFSQDVADMA
Sbjct: 61  VVPGDRLLVNSAILDCLPSLKLVVTTSAGVDHLDVAELRRRRVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR+GLWPTQGDFPL LKLSGK+IGIVGLGKIG+EVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRQGLWPTQGDFPLALKLSGKQIGIVGLGKIGTEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           C+ISYNSRTKKP + Y  YS++HELA NC+ALIICCGLT ET H+I+R+VMVALG+DGVI
Sbjct: 181 CKISYNSRTKKPTLPYPFYSDLHELAANCEALIICCGLTAETRHMIDRDVMVALGRDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           +N+GRGAI+DE  ++  LIQGE+ GAGLDVFE+EPE+PK+LFNLDNVVLSPHVAV TTES
Sbjct: 241 VNIGRGAIVDETELVRRLIQGELGGAGLDVFENEPEVPKELFNLDNVVLSPHVAVLTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLT+L + NLEAFFSNKPLVSP++D
Sbjct: 301 MVGLTQLALGNLEAFFSNKPLVSPYVD 327

BLAST of Lsi05G019850 vs. NCBI nr
Match: XP_038885023.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Benincasa hispida])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 304/327 (92.97%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAVE QS+ELPQVLVLS PT+F+SLESQFPNRFHFLKPW+SKLPLLQFLISNAQS  ACL
Sbjct: 1   MAVESQSDELPQVLVLSAPTVFSSLESQFPNRFHFLKPWDSKLPLLQFLISNAQSTRACL 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           VIPG RL++SSAILDCLPSLKL+VTTSAGVDHLHVA+LRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VIPGHRLSVSSAILDCLPSLKLVVTTSAGVDHLHVADLRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR+ LWPT GDFPLGLKLSGKRIGIVGLGKIGSEVAKRL GFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRQRLWPTLGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLVGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTK PLVLYSHYSNVHELATNC+ALIICCGLTEETHHLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKNPLVLYSHYSNVHELATNCEALIICCGLTEETHHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES
Sbjct: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLT+L VENLE FFSNKPLVSPFLD
Sbjct: 301 IVGLTKLAVENLEEFFSNKPLVSPFLD 327

BLAST of Lsi05G019850 vs. NCBI nr
Match: XP_008438122.1 (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo] >KAA0049014.1 glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa] >TYK17550.1 glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 1.9e-159
Identity = 293/327 (89.60%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAVE  S+EL QVLVLSPP  FTSLESQF NRF FL PWNS LPLLQFLISNAQS  ACL
Sbjct: 1   MAVEIPSDELHQVLVLSPPFEFTSLESQFQNRFQFLNPWNSNLPLLQFLISNAQSVRACL 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PG RLA+SSAILDCLPSL+L+VTTSAGVDHLHVAE+RRRGVAIA+AGNL SQDVADMA
Sbjct: 61  VMPGGRLAVSSAILDCLPSLELVVTTSAGVDHLHVAEIRRRGVAIAFAGNLLSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRN+SAGDRFVR+GLWPTQGDF +GLKLSGKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNISAGDRFVRQGLWPTQGDFSIGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YSHYSNVHELATNCDALIICC LTEET HLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDALIICCCLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIECLIQGEI+GAGLDVFE EPEIPKQLFNLDNVVLSPH A TTTES
Sbjct: 241 INVGRGAIIDEKAMIECLIQGEIKGAGLDVFEHEPEIPKQLFNLDNVVLSPHAAGTTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLTEL  ENLEAFFSNKPLVSPFLD
Sbjct: 301 IVGLTELAFENLEAFFSNKPLVSPFLD 327

BLAST of Lsi05G019850 vs. NCBI nr
Match: XP_004134341.2 (glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus] >KGN56573.1 hypothetical protein Csa_010304 [Cucumis sativus])

HSP 1 Score: 564.3 bits (1453), Expect = 6.8e-157
Identity = 291/327 (88.99%), Postives = 303/327 (92.66%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAV+ QS+EL QVLVLSPP++FTSLESQF NRF FLKPW+S LPLLQFLISNAQS  ACL
Sbjct: 1   MAVKIQSDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACL 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V PGD LA+SSAILDCLPSLK +VT SAGVDHL+VAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VTPGDGLAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR+GLW TQ DF LGLKL+GKRIGIVGLGKIGSEVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRQGLWATQMDFSLGLKLTGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YSHYSNVHELATNCD LIIC  LTEET HLINREVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAIIDEKAMIE LIQGEI+GAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES
Sbjct: 241 INVGRGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
             GL EL +ENLEAFFSNKPLVSPFLD
Sbjct: 301 IAGLIELALENLEAFFSNKPLVSPFLD 327

BLAST of Lsi05G019850 vs. NCBI nr
Match: KAG6597183.1 (Glyoxylate/hydroxypyruvate reductase HPR3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548.9 bits (1413), Expect = 3.0e-152
Identity = 277/327 (84.71%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAV RQ  ELPQVLVLSPP +FTSLESQF  RFH LKPW+SKLPLLQFL S AQS  A L
Sbjct: 1   MAVGRQPNELPQVLVLSPPAIFTSLESQFSIRFHMLKPWDSKLPLLQFLTSYAQSVPAML 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PGDRL ++SAILDCLPSLKL+VTTSAGVDHL VAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VVPGDRLLVNSAILDCLPSLKLVVTTSAGVDHLDVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR GLWPTQ DFPL LKLSGK+IGIVGLGKIG+EVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRLGLWPTQRDFPLALKLSGKQIGIVGLGKIGTEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YS+YSNVHELA NC+ALIICCGLT ET H+I+R+VMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVPYSYYSNVHELAANCEALIICCGLTAETRHMIDRDVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           +NVGRGAI+DEK +I CLIQGEIRGAGLDVFE++PEIPK+LFNLDNVVLSPHVAV TTES
Sbjct: 241 VNVGRGAIVDEKELIRCLIQGEIRGAGLDVFENDPEIPKELFNLDNVVLSPHVAVLTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLT+L + NLEAFFSNKPLVSP++D
Sbjct: 301 MVGLTQLALGNLEAFFSNKPLVSPYVD 327

BLAST of Lsi05G019850 vs. NCBI nr
Match: XP_022943936.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita moschata])

HSP 1 Score: 548.5 bits (1412), Expect = 3.9e-152
Identity = 278/327 (85.02%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACL 60
           MAV RQ  ELPQVLVLSPP +FTSLESQF  RFH LKPW+SKLPLLQFL S AQS  A L
Sbjct: 1   MAVGRQPNELPQVLVLSPPAIFTSLESQFSIRFHMLKPWDSKLPLLQFLTSYAQSVPAML 60

Query: 61  VIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMA 120
           V+PGDRL ++SAILDCLPSLKL+VTTSAGVDHL VAELRRRGVAIAYAGNLFSQDVADMA
Sbjct: 61  VVPGDRLLVNSAILDCLPSLKLVVTTSAGVDHLDVAELRRRGVAIAYAGNLFSQDVADMA 120

Query: 121 VGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180
           VGLLIDVLRNVSAGDRFVR GLWPTQ DFPL LKLSGK+IGIVGLGKIG+EVAKRLEGFG
Sbjct: 121 VGLLIDVLRNVSAGDRFVRLGLWPTQRDFPLALKLSGKQIGIVGLGKIGTEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVI 240
           CRISYNSRTKKPLV YS+YSNVHELA NC+ALIICCGLT ET H+I+REVMVALGKDGVI
Sbjct: 181 CRISYNSRTKKPLVSYSYYSNVHELAANCEALIICCGLTAETRHMIDREVMVALGKDGVI 240

Query: 241 INVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTES 300
           INVGRGAI+DE  +I CLIQGEIRGAGLDVFE++PEIPK+LFNLDNVVLSPHVAV TTES
Sbjct: 241 INVGRGAIVDETELIRCLIQGEIRGAGLDVFENDPEIPKELFNLDNVVLSPHVAVLTTES 300

Query: 301 TVGLTELVVENLEAFFSNKPLVSPFLD 328
            VGLT+L + NLEAFFSNKPLVSP++D
Sbjct: 301 MVGLTQLALGNLEAFFSNKPLVSPYVD 327

BLAST of Lsi05G019850 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 345.1 bits (884), Expect = 6.1e-95
Identity = 181/329 (55.02%), Postives = 237/329 (72.04%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQF--PNRFHFLKPWNSKLPLLQFLISNAQSALA 60
           MA    +E+LP+VL++  P     L   F    +F  LK + S LPL +FL  ++ S  A
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  CLVIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVAD 120
             +I      +++ ++  LP+L+L+VTTSAGVDH+ + E RRRG+++A AG+ FS+DVAD
Sbjct: 72  --IIAPVAAPVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 131

Query: 121 MAVGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEG 180
            AVGLLIDV R +SA +RFV++  WP +GD+PLG KL  KRIGIVGLG IGS+VA RL+ 
Sbjct: 132 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDA 191

Query: 181 FGCRISYNSRTKKPL-VLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKD 240
           FGC+ISY+SR +KP  V Y +Y ++ E+A N DALIICC L E+T  LIN++V+ ALGK 
Sbjct: 192 FGCQISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKR 251

Query: 241 GVIINVGRGAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTT 300
           GVI+NV RGAIIDE+ M+ CL +GEI GAGLDVFEDEP +PK+LF LDNVV SPH A  T
Sbjct: 252 GVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMT 311

Query: 301 TESTVGLTELVVENLEAFFSNKPLVSPFL 327
            E    L ++VV N+EAFFSNKPL++P L
Sbjct: 312 LEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

BLAST of Lsi05G019850 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 175/319 (54.86%), Postives = 221/319 (69.28%), Query Frame = 0

Query: 6   QSEELPQVLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGD 65
           +S E P VL+  PP+L T ++      F  L    S    L        S+    VI G 
Sbjct: 3   ESSEPPVVLLHRPPSL-TFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISG- 62

Query: 66  RLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLI 125
           RL ++  +L  LPSL++LV TS G+DH+ +A  +RRG+ I  AGN FS DVAD AVGLLI
Sbjct: 63  RLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLI 122

Query: 126 DVLRNVSAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISY 185
            VLR + A DR+VR G W   GDF LG K+SGKR+GIVGLG IGS VAKRLE FGC ISY
Sbjct: 123 SVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISY 182

Query: 186 NSRTKKPLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGR 245
           NSR++K    Y +YS++  LA N D L++CC LT+ETHH++NREVM  LGKDGV+INVGR
Sbjct: 183 NSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGR 242

Query: 246 GAIIDEKAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLT 305
           G +IDEK M++CL+ G I GAGLDVFE+EP +P++LF LDNVVLSPH AV T  S   + 
Sbjct: 243 GKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVA 302

Query: 306 ELVVENLEAFFSNKPLVSP 325
           ++ + NL+AFFSN+PL+SP
Sbjct: 303 QIALANLKAFFSNRPLLSP 319

BLAST of Lsi05G019850 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 255.8 bits (652), Expect = 4.8e-68
Identity = 133/313 (42.49%), Postives = 208/313 (66.45%), Query Frame = 0

Query: 13  VLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGDRLALSSA 72
           VL++ P + +  LE++   RF+ L+ W S  P    L+   ++++  +V  G+  A + A
Sbjct: 6   VLMMCPMSSY--LENELEKRFNLLRFWTS--PEKSVLLETHRNSIRAVV--GNASAGADA 65

Query: 73  -ILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNV 132
            ++  LP+L+++ + S G+D + + + + +G+ +    ++ ++DVAD+A+GL++ +LR +
Sbjct: 66  QLISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRL 125

Query: 133 SAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKK 192
              DR+VR G W  QG+F L  K SGK +GI+GLG+IG+ +AKR E F C I+Y SRT K
Sbjct: 126 CECDRYVRSGKW-KQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIK 185

Query: 193 PLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRGAIIDE 252
           P V Y +Y  V +LA N D L++ C LTE+T H+++R+VM ALG  GV+IN+GRG  +DE
Sbjct: 186 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 245

Query: 253 KAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTELVVEN 312
           + +I+ L +G + GA LDVFE EP +P++LF L+NVVL PHV   T E+   + +LVV N
Sbjct: 246 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 305

Query: 313 LEAFFSNKPLVSP 325
           LEA FS K L++P
Sbjct: 306 LEAHFSGKSLLTP 311

BLAST of Lsi05G019850 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 223.0 bits (567), Expect = 3.5e-58
Identity = 121/313 (38.66%), Postives = 193/313 (61.66%), Query Frame = 0

Query: 13  VLVLSPPTLFTSLESQFPNRFHFLKPWNSKLPLLQFLISNAQSALACLVIPGDRLALSSA 72
           VL++ P + +  LE++   RF+ L+ W S  P    L+   ++++  +V  G+  A + A
Sbjct: 6   VLMMCPMSSY--LENELEKRFNLLRFWTS--PEKSVLLETHRNSIRAVV--GNASAGADA 65

Query: 73  -ILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVADMAVGLLIDVLRNV 132
            ++  LP+L+++ + S G+D + + + + +G+ +    ++ ++DVAD+A+GL++ +LR +
Sbjct: 66  QLISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRL 125

Query: 133 SAGDRFVRRGLWPTQGDFPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRISYNSRTKK 192
              DR+VR G W                      G+IG+ +AKR E F C I+Y SRT K
Sbjct: 126 CECDRYVRSGKWKQ--------------------GRIGTAIAKRAEAFSCPINYYSRTIK 185

Query: 193 PLVLYSHYSNVHELATNCDALIICCGLTEETHHLINREVMVALGKDGVIINVGRGAIIDE 252
           P V Y +Y  V +LA N D L++ C LTE+T H+++R+VM ALG  GV+IN+GRG  +DE
Sbjct: 186 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 245

Query: 253 KAMIECLIQGEIRGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESTVGLTELVVEN 312
           + +I+ L +G + GA LDVFE EP +P++LF L+NVVL PHV   T E+   + +LVV N
Sbjct: 246 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 292

Query: 313 LEAFFSNKPLVSP 325
           LEA FS K L++P
Sbjct: 306 LEAHFSGKSLLTP 292

BLAST of Lsi05G019850 vs. TAIR 10
Match: AT2G45630.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 132.1 bits (331), Expect = 8.1e-31
Identity = 71/157 (45.22%), Postives = 104/157 (66.24%), Query Frame = 0

Query: 1   MAVERQSEELPQVLVLSPPTLFTSLESQF--PNRFHFLKPWNSKLPLLQFLISNAQSALA 60
           MA    +E+LP+VL++  P     L   F    +F  LK + S LPL +FL  ++ S  A
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  CLVIPGDRLALSSAILDCLPSLKLLVTTSAGVDHLHVAELRRRGVAIAYAGNLFSQDVAD 120
             +I      +++ ++  LP+L+L+VTTSAGVDH+ + E RRRG+++A AG+ FS+DVAD
Sbjct: 72  --IIAPVAAPVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVAD 131

Query: 121 MAVGLLIDVLRNVSAGDRFVRRGLWPTQGDFPLGLKL 156
            AVGLLIDV R +SA +RFV++  WP +GD+PLG K+
Sbjct: 132 TAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKV 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LE332.6e-9054.86Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR... [more]
Q65CJ71.7e-7045.19Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ... [more]
A5CAL12.8e-6842.00Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN... [more]
Q9CA906.8e-6742.49Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q5FTU62.6e-5035.002-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GO... [more]
Match NameE-valueIdentityDescription
A0A5D3D1Q79.3e-16089.60Glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3AW959.3e-16089.60glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0L4C23.3e-15788.99Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124920 PE=3 SV=1[more]
A0A6J1FT241.9e-15285.02glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IDA04.0e-14781.04glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_038885023.11.1e-16792.97glyoxylate/hydroxypyruvate reductase HPR3-like [Benincasa hispida][more]
XP_008438122.11.9e-15989.60PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo] >KAA004... [more]
XP_004134341.26.8e-15788.99glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus] >KGN56573.1 hypothet... [more]
KAG6597183.13.0e-15284.71Glyoxylate/hydroxypyruvate reductase HPR3, partial [Cucurbita argyrosperma subsp... [more]
XP_022943936.13.9e-15285.02glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G45630.26.1e-9555.02D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G12550.11.8e-9154.86D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.14.8e-6842.49D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.23.5e-5838.66D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT2G45630.18.1e-3145.22D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 49..321
e-value: 2.1E-12
score: 47.2
NoneNo IPR availableGENE3D3.40.50.720coord: 29..320
e-value: 7.3E-100
score: 335.4
NoneNo IPR availableGENE3D3.40.50.720coord: 113..297
e-value: 7.3E-100
score: 335.4
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 7..325
NoneNo IPR availablePANTHERPTHR10996:SF179D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN-RELATEDcoord: 7..325
NoneNo IPR availableCDDcd12156HPPRcoord: 11..317
e-value: 1.88587E-137
score: 389.906
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 21..149
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 121..294
e-value: 4.1E-51
score: 172.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 113..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G019850.1Lsi05G019850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0030267 glyoxylate reductase (NADP+) activity
molecular_function GO:0016618 hydroxypyruvate reductase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor