Lsi05G019540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G019540
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description2'-phosphotransferase
Locationchr05: 26675481 .. 26680296 (-)
RNA-Seq ExpressionLsi05G019540
SyntenyLsi05G019540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAATCTACACCGCATAAAGCATTCGTCTTCAACCTTTGTCCCTTCACGATGTGGGCTTCTGTTGTTACCAACTGCGGCATCAAAATTCTGCGCTGCCGTCCCCTTTTGCTTCCACGTGTCCTCATCACTCCAATTCGCCTGCCCATCTCTCCTTCTCTATTCTCCATGGATAGAAGCAATTCCTCCTCCTCTTCTTCTTTTGCCCATTCCAGTAAAAGGTTAATTATGCTCCTTCATCATCTTCATGGTTTTCTCTCCTCTGTTTTTCATACTTTAGAATCTGGAATTCAGATGGGTGTTTGTTGAAATTCTATAGGTGGTTATTGATTCTGTTATTTTTCTGATTCTTTCGATTAGTGGTGGAACGGGAAGAGGTTTGGATTTGAGGAATGATAGAGAAAGAACGAGAGGGCGTGGTGGAGGCGGCGGCGGCGGCGGCTCTGGTAAAGACAAAATTGACGCTCTTGGTAGACTCTTGTAAGCATATTTCGTCTATTGGTATCTTGTTTAGGTGTTAATTGCTTATTAAGCTTATGGAAGATTTTGCGGATGTTCTTTTATTTTGAGTTCTGTTGTTTATCTATGGTGAAACAAAGTATATCTACAATTGGTGATTCATGAGGTTGAAATTTCCTAGAATTTTGATTTTTTATTCTCTTTCTCTTCACTACTTGTTGGTTATGATCATTTTTCGAGGAAACATATAAAGGTACGCAGGATAGGAAGATCAAACTTTAAAATGGAAACCTTAAATCAAATGTACTTTAGCAGTTACGCACGTATAAGCTTGAGAAATTTTCAAAGGGAGTATAGTCTCATGATCTTAATGTTGTATTCCCTTTTGGTAGTGCTACCAATTGGCTTATTAACAATTTTGAGCTAAGTTGTTGTGAGGGTTCCTATATATTACTGTTTATGATTGGCTGGTGAAGAATGAATAAGCCCAAGTAGTAGATGTTTGAGAGGAATTCAATAGTTATGCATAATGCATATAGGATTTGTTTTTAACAGCAGTGTGATATGGTTTATTTTTTATTGATTGACGTTTTCCCATTTTAATGAACTTCAGGACTCGGATCTTGCGTCATATGGCTTCTGAGTTAAACCTGAATATGAGGGGCGATGGATATGTCAAGGTCCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCTAATATTCCACTGAGATTGCACACAATTGATGACATAAGAGAGGTTGGTCGAATAATTTTCTTCTTTACTTTCTATAGCATGACTCTAATTTGAAGAATCAAACTGATATGTAAGTTAGGTTTTCCGTTGCTTTCAAAAAAAAAAGTTAGGTTTTCCATTGCTCAACAACGTCATTGAATGTTGATTCGTCTATTTATCTTGATTCTTTACCTCTAGAGTATTATTTATCTGCTAAGATCAATTAAAACTTCACCACACGTATGCTCTACACTGCAGTTTTGATCCATTTATCCATGTTTTTTTTTTCTCAATTAGTTCCACTAATGTATTTCCTTTTCCCGGTGACAACTTGTTAATTTGCAGCTCATGTTTGTTTCTGTAAGCATCTATCGTTAAGGAATAGTTTTTTTTATTTAATTTCAGGCTGTTAGGAAGGATAATAAGCAGCGTTTCAGCCTCCTGGAAGAGAATGGAGAACTCTTAATTCGAGCAAACCAAGGCCATACAGTCACGGTTATTTTCTATCTTTATAAACCAGTGAATATGTTGTTGCCTTATACAAAATATTGGGGGACTATCAATCAAGCTGATTGATTGGAAAATTGTTGATTGCTTCACGTTAGGTTAAATGCATTTCATGCTTTTTAATATTTTGATTTATCTATGGAATGAAAAATCTATTTCACCTTGTTTGCTATCGTTTCTGTATTTTGTTGCAATCTATCAGCCTTGTAAGCTAGGTAAATTGTTGACTATATAGGATTGTTTATATAAGACTTCCCCACCTCTTGTAAGATTGTGATAATGGAACCAAATTGTCTATTTCGTCTACACCATCATAAATGGGAAAAACTTCCTTTTTAGGTGGTTGAAACTGAAAGCTTATTAAAGCCAATTCTTTCGGCCGAGGAGGTGCCAGGTACTCTGATTCAATGCCCTTAAATTTTCTACTTTTTTTTCCATATGTTAATCTTTCCTCCTTGATGTACATGATGTGAGTATAGATTCATTTTAAACTTCTACTTTTGTTGTTCAGTCAATCACTTTCTTTTTCTCCCTCTCTACATGACAGTATGTGTACACGGGACTTATAAGAAGAATTTGGAATCAATTCTGGAATCTGGTTTGAAACGTATGAAAAGATTGCACGTTCACTTCTCATGTGGTTTGCCAACGGATGGGGAGGTTATCAGTGGTATGTTAAGAAAATTTAACTGTTACATTTCCCTCAAGAATTTATCCGATAAGATCATGATGTCACTTTGATTGGCAATTAGTGGCTTCTGCAATTGCACATCTTAACCTATTTATCTCTTTGCAAATAGTAAACGAGGACGAGTCTGATAGGAGTGGTATTAGGTGTTAGAGAGATATTAAGGATATATTAGTAATTAGCTGTGGGAGTTAGTTATGGAATTTGGTTATAAATAGAGATAAGGAGAATAGGGAAGATAGCCAATATTTTGGAGCTTGAAAGTATCTCAAGATTGGGAGGATCCAAGTACCTCGAATACTTGGTTTTTTTTATAATTCCGTTATCATTTATCTTAAAATATATCCGGGTTCTATCTGAATCATATGGTTTGCATCTAAGTTTCTTCAAAAGTAAATGAAACAACTTAATTCATGTATTGCCGTTGATGTTAAAATTTTCTAGAGTAAAAGGAAAAGGATCCATAGTAGATTTTCTCAACCATTTTTCATATATCCATGATTATATAAGATAAAAGTAAAGGATGCTGACATGAATATGAGGCCATTGGTTCGCTTTACTTGCATTAGAAGGAAGAAGCTTGGATCTAAGAAGAAGTGCAAATGGGGTCTTGTGGGGAAAGGAAAGTAGTTAATTGGGGTAGTTAGGGTAATCTTTTAGTCATCGTTTGTTAATAATATATTATCATATCGAGGTTAGCAAACTAGTTCGAAGTTAGTTTGTTAGATGTTGAGAGCTGGGCAGGATTCTGAAATACTTACATCTACAAGAAGGGGAGAGGGTAAGAGTATGGTAGTTAGTTTTTAGTAAGTTGAAGATGGAAATAGCTTCTAGCCTTTGGAGAGACACACCTCTTGAACTTCTGTGAGATTTGTATCCTTTTCTTATAGTTAAAGAGGTATGTGTTAAATCACCCACCAACCCAAAAGCTTAAGTGGTTGGGTTTTAGTAAATTTAATTATAGGTTAAAATACAACTTTTGGTCTTTGAATTTTCATAAAAGTAACAATTTAGTTCCTGAACTATAGTTTGTAACGATTTAGTCTCTATACTTCTAAATTTGTTATAATTTAGTCCCTAAGCTTTAATAAGTAATGATTTAGTTCCTATAGTTTACAATTTGTAACGATTTAGTCTCTATTATGATAAATATTAGTATGATTTAATGAAATTTCTTACACATGTAGATTGATAAACTGATCGAAAATCAAATTTATTTATAAATTATAAAGACCATGTCATTATGTAATTAAAATTGACTTTTAATTCTGATGAAAATTTTCATGATAGGGACTAAGTCATTACTTACTAAAAGTTCAGAGACTAAGTTGTTACATACTTGATAGTATAGTGATTAATTCGTTACCAACTAAAGTTCAGGGATTAAATTGTTACTTTCATAGATGTTCAAGGACCAAAATTGTTTTTTAACCTTAATGATATTAGTACTTCCACACTATGTTTCTTCTCGAGGGTGTATCATATACTTAGTCTTGTAGTGAACCAAAATTGGGGAGACAAAGCTAACTTCTTGTTATTTTTCAAAATCTGACAGGTATGAGACGCAATGTCAATGTTTTAATATTTCTTGATGTCAAAAAAGCTCTGGATGGTACCGTTGAACACTCTTTTCTTTTCATTTAAATAATTAAGGTATAACAATAAGTGAATGAAACGTGATGATCATTCATCACTGATATCTGTTTTTGCTAAAACATTATATTTCAGATGGAATGAAGCTTTACATTTCAGAAAACAAGGTCATTCTTACTGAAGGCTTTGAAGGGGTGGTGGCAGTAAAATACTTCCAAAAGATTGAGTCATGGCCGAATCGACAACCTATTCCTTTTTGAGCCTAGATCGGATGGTACATGAACATGATTAATCATTTCTTGAAAATATTTATTAAAACATTCAATGTTCGATATGAACCAGATTGTCCTGTGCTAACTTTAATAGTTTAATGGCACAAGTTTCATTTGGATTTGGAACTTGGGAAATCTATGCATTGAACTTGGCTGGGAACAGAACCGTCCTTCATATGAACTTCTTGGTGAAATCATTCATGAAGGTTTGGCAACAAGTACAGATTTCTTTGTGAAGTAATGTTTAATGTCACAAGTTCACTGATGATGCCTGCTTCATTGGAGACTTGATATGAGAATTTACGTTCGGTTCGGTTTGTCTCAGTCAGTCATGGGTATTGTATCACTAAAGATTCTTAGGTATACAAACATATACACAGGACTTAGTTTTGTTTGTATATTGACTCATTATTTGATATGTTTTGATGGTGGTTGGAAATTTTAGTTTCTATTCTTTGTGATATTTTTAATTGCCATTAGCTTAATTGGTTAAATATCATACTCTTGATTAAGATGTCTAGTTTAATTTTAAATTTACATTCAAATGTAAACATTCT

mRNA sequence

ATGGCAAAATCTACACCGCATAAAGCATTCGTCTTCAACCTTTGTCCCTTCACGATGTGGGCTTCTGTTGTTACCAACTGCGGCATCAAAATTCTGCGCTGCCGTCCCCTTTTGCTTCCACGTGTCCTCATCACTCCAATTCGCCTGCCCATCTCTCCTTCTCTATTCTCCATGGATAGAAGCAATTCCTCCTCCTCTTCTTCTTTTGCCCATTCCAGTAAAAGTGGTGGAACGGGAAGAGGTTTGGATTTGAGGAATGATAGAGAAAGAACGAGAGGGCGTGGTGGAGGCGGCGGCGGCGGCGGCTCTGGTAAAGACAAAATTGACGCTCTTGGTAGACTCTTGACTCGGATCTTGCGTCATATGGCTTCTGAGTTAAACCTGAATATGAGGGGCGATGGATATGTCAAGGTCCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCTAATATTCCACTGAGATTGCACACAATTGATGACATAAGAGAGGCTGTTAGGAAGGATAATAAGCAGCGTTTCAGCCTCCTGGAAGAGAATGGAGAACTCTTAATTCGAGCAAACCAAGGCCATACAGTCACGGTTATTTTCTATCTTTATAAACCAGTGAATATGTTGTTGCCTTATACAAAATATTGGGGGACTATCAATCAAGCTGATTGATTGGAAAATTGTTGATTGCTTCACGTTAGGTTAAATGCATTTCATGCTTTTTAATATTTTGATTTATCTATGGAATGAAAAATCTATTTCACCTTGTTTGCTATCGTTTCTGTATTTTGTTGCAATCTATCAGCCTTGTAAGCTAGGTAAATTGTTGACTATATAGGATTGTTTATATAAGACTTCCCCACCTCTTGTAAGATTGTGATAATGGAACCAAATTGTCTATTTCGTCTACACCATCATAAATGGGAAAAACTTCCTTTTTAGGTGGTTGAAACTGAAAGCTTATTAAAGCCAATTCTTTCGGCCGAGGAGGTGCCAGTATGTGTACACGGGACTTATAAGAAGAATTTGGAATCAATTCTGGAATCTGGTTTGAAACGTATGAAAAGATTGCACGTTCACTTCTCATGTGGTTTGCCAACGGATGGGGAGGTTATCAGTGGTATGAGACGCAATGTCAATGTTTTAATATTTCTTGATGTCAAAAAAGCTCTGGATGATGGAATGAAGCTTTACATTTCAGAAAACAAGGTCATTCTTACTGAAGGCTTTGAAGGGGTGGTGGCAGTAAAATACTTCCAAAAGATTGAGTCATGGCCGAATCGACAACCTATTCCTTTTTGAGCCTAGATCGGATGGTACATGAACATGATTAATCATTTCTTGAAAATATTTATTAAAACATTCAATGTTCGATATGAACCAGATTGTCCTGTGCTAACTTTAATAGTTTAATGGCACAAGTTTCATTTGGATTTGGAACTTGGGAAATCTATGCATTGAACTTGGCTGGGAACAGAACCGTCCTTCATATGAACTTCTTGGTGAAATCATTCATGAAGGTTTGGCAACAAGTACAGATTTCTTTGTGAAGTAATGTTTAATGTCACAAGTTCACTGATGATGCCTGCTTCATTGGAGACTTGATATGAGAATTTACGTTCGGTTCGGTTTGTCTCAGTCAGTCATGGGTATTGTATCACTAAAGATTCTTAGGTATACAAACATATACACAGGACTTAGTTTTGTTTGTATATTGACTCATTATTTGATATGTTTTGATGGTGGTTGGAAATTTTAGTTTCTATTCTTTGTGATATTTTTAATTGCCATTAGCTTAATTGGTTAAATATCATACTCTTGATTAAGATGTCTAGTTTAATTTTAAATTTACATTCAAATGTAAACATTCT

Coding sequence (CDS)

ATGGCAAAATCTACACCGCATAAAGCATTCGTCTTCAACCTTTGTCCCTTCACGATGTGGGCTTCTGTTGTTACCAACTGCGGCATCAAAATTCTGCGCTGCCGTCCCCTTTTGCTTCCACGTGTCCTCATCACTCCAATTCGCCTGCCCATCTCTCCTTCTCTATTCTCCATGGATAGAAGCAATTCCTCCTCCTCTTCTTCTTTTGCCCATTCCAGTAAAAGTGGTGGAACGGGAAGAGGTTTGGATTTGAGGAATGATAGAGAAAGAACGAGAGGGCGTGGTGGAGGCGGCGGCGGCGGCGGCTCTGGTAAAGACAAAATTGACGCTCTTGGTAGACTCTTGACTCGGATCTTGCGTCATATGGCTTCTGAGTTAAACCTGAATATGAGGGGCGATGGATATGTCAAGGTCCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCTAATATTCCACTGAGATTGCACACAATTGATGACATAAGAGAGGCTGTTAGGAAGGATAATAAGCAGCGTTTCAGCCTCCTGGAAGAGAATGGAGAACTCTTAATTCGAGCAAACCAAGGCCATACAGTCACGGTTATTTTCTATCTTTATAAACCAGTGAATATGTTGTTGCCTTATACAAAATATTGGGGGACTATCAATCAAGCTGATTGA

Protein sequence

MAKSTPHKAFVFNLCPFTMWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGTGRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVIFYLYKPVNMLLPYTKYWGTINQAD
Homology
[Close]
BLAST of Lsi05G019540 vs. ExPASy Swiss-Prot
Match: Q5EAR5 (tRNA 2'-phosphotransferase 1 OS=Danio rerio OX=7955 GN=trpt1 PE=2 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 43/107 (40.19%), Postives = 63/107 (58.88%), Query Frame = 0 

Query: 91  TRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFA 150
           TRGRG  G G  + + +   L + L+ +LRH AS++ L M  DG+V V++LL       A
Sbjct: 5   TRGRGRRGRGNRNEESRDVRLSKSLSYVLRHGASKMGLQMNSDGFVFVEELL-------A 64

Query: 151 NIPLRLHTIDDIREAVRKDNKQRFSLLE--ENGELLIRANQGHTVTV 196
           +   R  ++DD+   V  ++KQRF L +  E+  L IRANQGH+V V
Sbjct: 65  HQQFRSFSVDDVERVVASNDKQRFKLCKHPEDDRLQIRANQGHSVQV 104

[Close]
BLAST of Lsi05G019540 vs. ExPASy Swiss-Prot
Match: Q8K3A2 (tRNA 2'-phosphotransferase 1 OS=Mus musculus OX=10090 GN=Trpt1 PE=2 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 1.4e-07
Identity = 43/115 (37.39%), Postives = 61/115 (53.04%), Query Frame = 0 

Query: 105 KDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFANIPLRLHTIDDIRE 164
           +D+   L + L+  LRH A +L L MR DG+V +Q LL+L             +I+D++ 
Sbjct: 20  QDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLP-------QFHSFSIEDVQL 79

Query: 165 AVRKDNKQRFSLL--EENGELLIRANQGHTVTVIFYLYKPVN--MLLPYTKYWGT 216
            V  + KQRF+L   E +  LLIRANQGH++ V      P+     LP T   GT
Sbjct: 80  VVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGT 127

[Close]
BLAST of Lsi05G019540 vs. ExPASy Swiss-Prot
Match: O14045 (Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2C4.12c PE=3 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 4.1e-07
Identity = 30/84 (35.71%), Postives = 50/84 (59.52%), Query Frame = 0 

Query: 113 RLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQ 172
           + L+++LRH A    L +R DGY++V  +LK  L  F  + + L     +   V+ ++K+
Sbjct: 43  KALSKVLRHTAKANGLQIREDGYIEVDSILK--LPQFRGMGMEL-----LLSIVKGNDKK 102

Query: 173 RFSLLEENGELLIRANQGHTVTVI 197
           RF++ E  G L IRANQGH++  +
Sbjct: 103 RFTMEEVEGVLYIRANQGHSIKAV 119

[Close]
BLAST of Lsi05G019540 vs. ExPASy Swiss-Prot
Match: O29841 (Probable RNA 2'-phosphotransferase 1 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=kptA1 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 5.0e-05
Identity = 30/93 (32.26%), Postives = 51/93 (54.84%), Query Frame = 0 

Query: 105 KDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTF--ANIPLRLHTIDDI 164
           K+K++ LG+ ++ +LRH   +  LNM  +G+V ++ L ++  + +  ANI L       I
Sbjct: 30  KEKVEKLGKFISGVLRHFPDKFGLNMDENGWVNLESLARVVKRRYKWANIWL-------I 89

Query: 165 REAVRKDNKQRFSLLEENGELLIRANQGHTVTV 196
           +  V  D KQR+ L  +     IRA  GH++ V
Sbjct: 90  KALVYSDEKQRYELKGDK----IRARYGHSIDV 111

[Close]
BLAST of Lsi05G019540 vs. ExPASy Swiss-Prot
Match: Q3ZBM7 (tRNA 2'-phosphotransferase 1 OS=Bos taurus OX=9913 GN=TRPT1 PE=2 SV=3)

HSP 1 Score: 49.7 bits (117), Expect = 5.0e-05
Identity = 39/118 (33.05%), Postives = 59/118 (50.00%), Query Frame = 0 

Query: 105 KDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFANIPLRLHTIDDIRE 164
           +D+   L + L+  LRH A +L L M  DG+V +  LL+L          R  + +D++ 
Sbjct: 26  QDRDVQLSKALSYALRHGALKLGLPMGADGFVPLDALLQLP-------QFRSFSAEDVQR 85

Query: 165 AVRKDNKQRFSLL--EENGELLIRANQGHTVTVIFYLYKPVN--MLLPYTKYWGTINQ 219
            V  + KQRF+L   + +   LIRANQGH++ V     +P+     LP     GT  Q
Sbjct: 86  VVDTNVKQRFALQPGDPSTGPLIRANQGHSLQVPELELEPLETPQALPLMLVHGTFRQ 136

[Close]
BLAST of Lsi05G019540 vs. ExPASy TrEMBL
Match: A0A5D3D3K0 (2'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003870 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.2e-80
Identity = 164/178 (92.13%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCR LLLPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFSLL+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. ExPASy TrEMBL
Match: A0A5A7U5Z1 (2'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002720 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.2e-80
Identity = 164/178 (92.13%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCR LLLPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFSLL+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. ExPASy TrEMBL
Match: A0A1S3AVU4 (2'-phosphotransferase OS=Cucumis melo OX=3656 GN=LOC103483358 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.2e-80
Identity = 164/178 (92.13%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCR LLLPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFSLL+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. ExPASy TrEMBL
Match: A0A0A0L3T7 (2'-phosphotransferase OS=Cucumis sativus OX=3659 GN=Csa_3G126200 PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 3.2e-79
Identity = 161/178 (90.45%), Postives = 167/178 (93.82%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCRPL LPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRPLWLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GR LDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTR+LRHMASELNLNMR DGYVKV
Sbjct: 61  GRDLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRVLRHMASELNLNMRSDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFS L+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSFLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. ExPASy TrEMBL
Match: A0A6J1CZ57 (2'-phosphotransferase OS=Momordica charantia OX=3673 GN=LOC111016108 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 8.1e-75
Identity = 157/178 (88.20%), Postives = 163/178 (91.57%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWA  VTNCGIKILR RPLLLPRVLITPI LPI P++FSMD  N+SS +SFAHSSKSGG 
Sbjct: 1   MWA-CVTNCGIKILRYRPLLLPRVLITPIPLPIPPAVFSMDNPNTSSFASFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLD+ NDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDMTNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           QDLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRF LLEENGELLIRANQGHTVTV+
Sbjct: 121 QDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVV 175

[Close]
BLAST of Lsi05G019540 vs. NCBI nr
Match: KAA0049045.1 (Phosphotransferase KptA/Tpt1 [Cucumis melo var. makuwa])

HSP 1 Score: 308.5 bits (789), Expect = 4.5e-80
Identity = 164/178 (92.13%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCR LLLPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFSLL+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. NCBI nr
Match: XP_008438171.1 (PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis melo])

HSP 1 Score: 308.5 bits (789), Expect = 4.5e-80
Identity = 164/178 (92.13%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCR LLLPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFSLL+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. NCBI nr
Match: TYK17519.1 (tRNA 2'-phosphotransferase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 308.5 bits (789), Expect = 4.5e-80
Identity = 164/178 (92.13%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCR LLLPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFSLL+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. NCBI nr
Match: XP_038893116.1 (tRNA 2'-phosphotransferase 1 [Benincasa hispida])

HSP 1 Score: 307.0 bits (785), Expect = 1.3e-79
Identity = 162/178 (91.01%), Postives = 168/178 (94.38%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCRPLLLPRVLI PI LP+SPSLF+MDR N+SSSSSFAHSS+SGG 
Sbjct: 1   MWASVVTNCGIKILRCRPLLLPRVLINPIPLPVSPSLFAMDRINTSSSSSFAHSSRSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GRGLDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTRILRHMA+ELNLNMR DGYVKV
Sbjct: 61  GRGLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRILRHMAAELNLNMRSDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           QDLLKLNLKTFAN PLR HTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTV V+
Sbjct: 121 QDLLKLNLKTFANTPLRSHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVAVV 176

[Close]
BLAST of Lsi05G019540 vs. NCBI nr
Match: XP_004133917.2 (tRNA 2'-phosphotransferase 1 isoform X1 [Cucumis sativus] >KGN56605.1 hypothetical protein Csa_009546 [Cucumis sativus])

HSP 1 Score: 304.7 bits (779), Expect = 6.6e-79
Identity = 161/178 (90.45%), Postives = 167/178 (93.82%), Query Frame = 0 

Query: 19  MWASVVTNCGIKILRCRPLLLPRVLITPIRLPISPSLFSMDRSNSSSSSSFAHSSKSGGT 78
           MWASVVTNCGIKILRCRPL LPRVLITPI LPISPSL SMDRSN++SSSSFAHSSKSGG 
Sbjct: 1   MWASVVTNCGIKILRCRPLWLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 79  GRGLDLRNDRERTRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKV 138
           GR LDLRNDRERTRGR  GGGGGGSGKDKIDALGRLLTR+LRHMASELNLNMR DGYVKV
Sbjct: 61  GRDLDLRNDRERTRGR--GGGGGGSGKDKIDALGRLLTRVLRHMASELNLNMRSDGYVKV 120

Query: 139 QDLLKLNLKTFANIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           +DLLKLNLKTFANIPLR HTIDDIREAVRKDNKQRFS L+ENGELLIRANQGHTVT +
Sbjct: 121 EDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSFLDENGELLIRANQGHTVTAV 176

[Close]
BLAST of Lsi05G019540 vs. TAIR 10
Match: AT2G45330.1 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 185.3 bits (469), Expect = 5.4e-47
Identity = 107/166 (64.46%), Postives = 126/166 (75.90%), Query Frame = 0 

Query: 46  PIRLPISPSLF----------SMDRSNSSSS-----SSFAHSSKSGGTGRGLDLRNDRER 105
           P+ L +S S+F           MD SN +SS     SSFA SS+SGG G G +  NDR R
Sbjct: 11  PLPLTLSSSVFFSKSSPVSSAFMDASNPNSSRKSNVSSFAQSSRSGGRGGGYERDNDRRR 70

Query: 106 TRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFA 165
            +GRG GGG    GKD+IDALGRLLTRILRHMA+EL LNMRGDG+VKV+DLL LNLKT A
Sbjct: 71  PQGRGDGGG----GKDRIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLNLNLKTSA 130

Query: 166 NIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           NI L+ HTID+IREAVR+DNKQRFSL++ENGELLIRANQGH++T +
Sbjct: 131 NIQLKSHTIDEIREAVRRDNKQRFSLIDENGELLIRANQGHSITTV 172

[Close]
BLAST of Lsi05G019540 vs. TAIR 10
Match: AT2G45330.2 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-44
Identity = 105/166 (63.25%), Postives = 124/166 (74.70%), Query Frame = 0 

Query: 46  PIRLPISPSLF----------SMDRSNSSSS-----SSFAHSSKSGGTGRGLDLRNDRER 105
           P+ L +S S+F           MD SN +SS     SSFA SS+ GG   G +  NDR R
Sbjct: 11  PLPLTLSSSVFFSKSSPVSSAFMDASNPNSSRKSNVSSFAQSSRRGG---GYERDNDRRR 70

Query: 106 TRGRGGGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFA 165
            +GRG GGG    GKD+IDALGRLLTRILRHMA+EL LNMRGDG+VKV+DLL LNLKT A
Sbjct: 71  PQGRGDGGG----GKDRIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLNLNLKTSA 130

Query: 166 NIPLRLHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           NI L+ HTID+IREAVR+DNKQRFSL++ENGELLIRANQGH++T +
Sbjct: 131 NIQLKSHTIDEIREAVRRDNKQRFSLIDENGELLIRANQGHSITTV 169

[Close]
BLAST of Lsi05G019540 vs. TAIR 10
Match: AT5G23600.1 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 146.0 bits (367), Expect = 3.6e-35
Identity = 69/91 (75.82%), Postives = 83/91 (91.21%), Query Frame = 0 

Query: 106 DKIDALGRLLTRILRHMASELNLNMRGDGYVKVQDLLKLNLKTFANIPLRLHTIDDIREA 165
           D+IDALGR+LTRILRHMA+EL LNMRGDG+VKV+DLL LNLKT ANI L  HTID+IREA
Sbjct: 5   DRIDALGRILTRILRHMATELRLNMRGDGFVKVEDLLNLNLKTCANIQLNSHTIDEIREA 64

Query: 166 VRKDNKQRFSLLEENGELLIRANQGHTVTVI 197
           V +DNK+RFSL++E+GELLIRANQGH++T +
Sbjct: 65  VTRDNKKRFSLIDEDGELLIRANQGHSITTV 95

The following BLAST results are available for this feature:
BLAST of Lsi05G019540 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q5EAR53.0e-1040.19tRNA 2'-phosphotransferase 1 OS=Danio rerio OX=7955 GN=trpt1 PE=2 SV=2[more]
Q8K3A21.4e-0737.39tRNA 2'-phosphotransferase 1 OS=Mus musculus OX=10090 GN=Trpt1 PE=2 SV=2[more]
O140454.1e-0735.71Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / A... [more]
O298415.0e-0532.26Probable RNA 2'-phosphotransferase 1 OS=Archaeoglobus fulgidus (strain ATCC 4955... [more]
Q3ZBM75.0e-0533.05tRNA 2'-phosphotransferase 1 OS=Bos taurus OX=9913 GN=TRPT1 PE=2 SV=3[more]
BLAST of Lsi05G019540 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3D3K02.2e-8092.132'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold43... [more]
A0A5A7U5Z12.2e-8092.132'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17... [more]
A0A1S3AVU42.2e-8092.132'-phosphotransferase OS=Cucumis melo OX=3656 GN=LOC103483358 PE=3 SV=1[more]
A0A0A0L3T73.2e-7990.452'-phosphotransferase OS=Cucumis sativus OX=3659 GN=Csa_3G126200 PE=3 SV=1[more]
A0A6J1CZ578.1e-7588.202'-phosphotransferase OS=Momordica charantia OX=3673 GN=LOC111016108 PE=3 SV=1[more]
BLAST of Lsi05G019540 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0049045.14.5e-8092.13Phosphotransferase KptA/Tpt1 [Cucumis melo var. makuwa][more]
XP_008438171.14.5e-8092.13PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis melo][more]
TYK17519.14.5e-8092.13tRNA 2'-phosphotransferase 1 [Cucumis melo var. makuwa][more]
XP_038893116.11.3e-7991.01tRNA 2'-phosphotransferase 1 [Benincasa hispida][more]
XP_004133917.26.6e-7990.45tRNA 2'-phosphotransferase 1 isoform X1 [Cucumis sativus] >KGN56605.1 hypothetic... [more]
BLAST of Lsi05G019540 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45330.15.4e-4764.46RNA 2'-phosphotransferase, Tpt1 / KptA family [more]
AT2G45330.21.1e-4463.25RNA 2'-phosphotransferase, Tpt1 / KptA family [more]
AT5G23600.13.6e-3575.82RNA 2'-phosphotransferase, Tpt1 / KptA family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002745Phosphotransferase KptA/Tpt1PFAMPF01885PTS_2-RNAcoord: 109..215
e-value: 1.5E-24
score: 87.0
IPR002745Phosphotransferase KptA/Tpt1PANTHERPTHR12684PUTATIVE PHOSPHOTRANSFERASEcoord: 89..195
IPR042080RNA 2'-phosphotransferase, N-terminal domainGENE3D1.10.10.970coord: 107..187
e-value: 1.4E-17
score: 65.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..105
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 111..216

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G019540.1Lsi05G019540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
molecular_function GO:0000215 tRNA 2'-phosphotransferase activity
molecular_function GO:0016740 transferase activity