Lsi05G018940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G018940
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	PMEI domain-containing protein
Location	chr05: 26094970 .. 26095530 (+)
RNA-Seq Expression	Lsi05G018940
Synteny	Lsi05G018940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTTCGCTTATACGCACCGTTTCATCGCGCTGGCATTTTCATTCTTTATCGTCTTCAATTTCCAATCCATTGCCGCAAACGACATCGTTTTACAAACCTGCGAAATCTCCGCCGCAAGCGATCCGAACGTCCGCCTCGACTTCTGTCTCCGATCTCTCGCCGCTGCTCCCGTGAGCGACACGGCGGACCTTTACGAACTCGGCGCGATCTCGATCAATTTGATCGGTAGGAATGCGACCAGTACGCGGCGGTACATCGAGCGATTACTGAAGAAGAAGAAGAAGCTGTCGCCGGATTCGTACGTGAGGCCGCGGCTGAGCGATTGCAAAGAACTCTACTCTGACGCTGTCGAGACGGTGGGCGATGCGGCGGCGGAGTACGCTCGTAAGCGCTATGAAGAAGCGAATGTGAAGTTGAGTTCGGTTATGGATGCTGTTACGACTTGTGAGGACGGATTCAAAGAAATTGAAGGCGGAGTTTCGCCATTGACGAAGCAAAATGGTGATGCGTTCGAGTTAACCGCCATTGCGCTCTGTATTCTGAACATGCGTCCTTGA

mRNA sequence

ATGAGTTTCGCTTATACGCACCGTTTCATCGCGCTGGCATTTTCATTCTTTATCGTCTTCAATTTCCAATCCATTGCCGCAAACGACATCGTTTTACAAACCTGCGAAATCTCCGCCGCAAGCGATCCGAACGTCCGCCTCGACTTCTGTCTCCGATCTCTCGCCGCTGCTCCCGTGAGCGACACGGCGGACCTTTACGAACTCGGCGCGATCTCGATCAATTTGATCGGTAGGAATGCGACCAGTACGCGGCGGTACATCGAGCGATTACTGAAGAAGAAGAAGAAGCTGTCGCCGGATTCGTACGTGAGGCCGCGGCTGAGCGATTGCAAAGAACTCTACTCTGACGCTGTCGAGACGGTGGGCGATGCGGCGGCGGAGTACGCTCGTAAGCGCTATGAAGAAGCGAATGTGAAGTTGAGTTCGGTTATGGATGCTGTTACGACTTGTGAGGACGGATTCAAAGAAATTGAAGGCGGAGTTTCGCCATTGACGAAGCAAAATGGTGATGCGTTCGAGTTAACCGCCATTGCGCTCTGTATTCTGAACATGCGTCCTTGA

Coding sequence (CDS)

ATGAGTTTCGCTTATACGCACCGTTTCATCGCGCTGGCATTTTCATTCTTTATCGTCTTCAATTTCCAATCCATTGCCGCAAACGACATCGTTTTACAAACCTGCGAAATCTCCGCCGCAAGCGATCCGAACGTCCGCCTCGACTTCTGTCTCCGATCTCTCGCCGCTGCTCCCGTGAGCGACACGGCGGACCTTTACGAACTCGGCGCGATCTCGATCAATTTGATCGGTAGGAATGCGACCAGTACGCGGCGGTACATCGAGCGATTACTGAAGAAGAAGAAGAAGCTGTCGCCGGATTCGTACGTGAGGCCGCGGCTGAGCGATTGCAAAGAACTCTACTCTGACGCTGTCGAGACGGTGGGCGATGCGGCGGCGGAGTACGCTCGTAAGCGCTATGAAGAAGCGAATGTGAAGTTGAGTTCGGTTATGGATGCTGTTACGACTTGTGAGGACGGATTCAAAGAAATTGAAGGCGGAGTTTCGCCATTGACGAAGCAAAATGGTGATGCGTTCGAGTTAACCGCCATTGCGCTCTGTATTCTGAACATGCGTCCTTGA

Protein sequence

MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYELGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNMRP

Homology

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BLAST of Lsi05G018940 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-30
Identity = 77/176 (43.75%), Postives = 108/176 (61.36%), Query Frame = 0


Query: 9   FIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYEL 68
           F    F F ++   Q++ + DIV  TC+  A   PNV  DFC++SL A P S TADL  L
Sbjct: 5   FSLCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGL 64

Query: 69  GAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAAEY 128
           G IS NL  ++ +  + +I R+LK K     D  ++  L+DC  LY+DA  +V +A A++
Sbjct: 65  GVISANLAIQHGSKIQTFIGRILKSK----VDPALKKYLNDCVGLYADAKSSVQEAIADF 124

Query: 129 ARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNM 185
             K Y  ANVK+S+ +D   TCEDGFKE +G VSP+TK+N D  +LTAI+L I  +
Sbjct: 125 KSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

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BLAST of Lsi05G018940 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.6e-28
Identity = 76/173 (43.93%), Postives = 105/173 (60.69%), Query Frame = 0


Query: 12  LAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYELGAI 71
           L+FS  I F    + + DIV  TC+  A   PNV  DFC++SL A P S +ADL  LG I
Sbjct: 3   LSFSLCIFF----LISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVI 62

Query: 72  SINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAAEYARK 131
           S NL  +  +  + +I R+LK K     D  ++  L+DC  LY+DA  +V +A A++  K
Sbjct: 63  SANLAIQQGSKIQTFIGRILKSK----VDPALKKYLNDCVGLYADAKSSVQEAIADFKSK 122

Query: 132 RYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNM 185
            Y  ANVK+S+ +D   TCEDGFKE +G  SP+TK+N D  +LTAI+L I  +
Sbjct: 123 DYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of Lsi05G018940 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.6e-10
Identity = 47/159 (29.56%), Postives = 73/159 (45.91%), Query Frame = 0


Query: 27  ANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYE-LGAISINLIGRNATSTRR 86
           A  ++  +C+ + A DP +  DFC+ SL   P S  A   E L   S        T+ + 
Sbjct: 20  AQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTKTAAAKITNLKG 79

Query: 87  YIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAAEYARKRYEEANVKLSSVMD 146
            + + LK ++       +   L  C   Y+DA + +  A A    + Y+ AN+ LS+ +D
Sbjct: 80  IVAQDLKDQRYQD----IVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALD 139

Query: 147 AVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNM 185
               CED FKE +   SP+T +N   F+   I L   NM
Sbjct: 140 VPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

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BLAST of Lsi05G018940 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.6e-07
Identity = 47/186 (25.27%), Postives = 80/186 (43.01%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           M+F+Y    + ++    I+F         +  +   IS           CL++L + P S
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120
            + DL  LG  SI++   +A  T + I  L  +    + D  ++ R   C E Y+DA+++
Sbjct: 61  ASKDLKGLGQFSIDIAQASAKQTSKIIASLTNQ----ATDPKLKGRYETCSENYADAIDS 120

Query: 121 VGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALC 180
           +G A        Y   N+  S+  D   TCED F+      + L + +    +L  I L 
Sbjct: 121 LGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLV 180

Query: 181 ILNMRP 187
           I N+ P
Sbjct: 181 ISNLLP 182

[Close]

BLAST of Lsi05G018940 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.2e-05
Identity = 43/184 (23.37%), Postives = 79/184 (42.93%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           M+   T+R +  +  FF++          +  Q  +I A         FC   + + P +
Sbjct: 1   MAAYLTNRVLMSSLMFFVM-------TGSLNAQVADIKAICGKAKNQSFCTSYMKSNPKT 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120
             ADL  L  I+      +A+   R I+ L+    K + +  ++   + C + Y  A+ +
Sbjct: 61  SGADLQTLANITFGSAQTSASEGFRKIQSLV----KTATNPTMKKAYTSCVQHYKSAISS 120

Query: 121 VGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALC 180
           + DA    A    +  N+K+S+ M+  +TCE    + +   S + K +GD   +  I L 
Sbjct: 121 LNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSAV-KNSGDFQNICGIVLV 172

Query: 181 ILNM 185
           I NM
Sbjct: 181 ISNM 172

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BLAST of Lsi05G018940 vs. ExPASy TrEMBL
Match: A0A1S3AVZ7 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103483434 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.6e-77
Identity = 153/187 (81.82%), Postives = 169/187 (90.37%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MSF YTHR+IALAFSF ++ NF+ I+ANDIV +TCE SAA DPNVRLDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRYIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLK-KKKKLSPDSYVRPRLSDCKELYSDAVE 120
           DTADLYELGAISI LIGRNATST+RYIERLLK +KKK S DSY+RPRLSDC+ELYSDAVE
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIAL 180
           TVG+AAAEY RKRY+E NVKLSSVMDAVTTCEDGFKE+E  VSPLTK+NGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 187
           CIL++RP
Sbjct: 181 CILDLRP 187

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BLAST of Lsi05G018940 vs. ExPASy TrEMBL
Match: A0A5A7U1K7 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003190 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.1e-76
Identity = 153/187 (81.82%), Postives = 168/187 (89.84%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MSF YTHR IALAFSF ++ NF+ I+ANDIV +TCE SAA DPNVRLDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRCIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLK-KKKKLSPDSYVRPRLSDCKELYSDAVE 120
           DTADLYELGAISI LIGRNATST+RYIERLLK +KKK S DSY+RPRLSDC+ELYSDAVE
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIAL 180
           TVG+AAAEY RKRY+E NVKLSSVMDAVTTCEDGFKE+E  VSPLTK+NGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 187
           CIL++RP
Sbjct: 181 CILDLRP 187

[Close]

BLAST of Lsi05G018940 vs. ExPASy TrEMBL
Match: A0A6J1E955 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111431821 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.7e-73
Identity = 150/189 (79.37%), Postives = 161/189 (85.19%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MS  YTHRFIALA S F+     S  AND+V +TCEISAA DPNV LDFCLRSLAAAP S
Sbjct: 1   MSCNYTHRFIALASSLFVALYLTSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPAS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKKKKKL---SPDSYVRPRLSDCKELYSDA 120
           DTADLYELGAISI LIGRNATSTRRYIERLLKKKKK    SPDSY+RP L+DC+ELYSD 
Sbjct: 61  DTADLYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDV 120

Query: 121 VETVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAI 180
             TV DAAA+YA KRYEE NVKLSS+MDAVTTCEDGFKE++GGVSPLTK+N DAFELTAI
Sbjct: 121 RVTVRDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAI 180

Query: 181 ALCILNMRP 187
           ALCILNMRP
Sbjct: 181 ALCILNMRP 189

[Close]

BLAST of Lsi05G018940 vs. ExPASy TrEMBL
Match: A0A0A0L7T4 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128870 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.4e-72
Identity = 146/186 (78.49%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MS  Y HRFIALA SFF++FN +SI+AND + + CE+SAASDPNVRLDFCL+SLAAAP S
Sbjct: 1   MSITYMHRFIALALSFFVLFNLRSISANDTLSRACELSAASDPNVRLDFCLQSLAAAPGS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120
           DTADLYELGA+SI LI  NATSTRRYIERLLK +KK SPD YVRPRLSDC+ELY DA++ 
Sbjct: 61  DTADLYELGALSIKLIAWNATSTRRYIERLLKNEKK-SPDPYVRPRLSDCEELYIDAIKA 120

Query: 121 VGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALC 180
           VGDAA EY R RYEE NVKLSSVMDAVTTCEDGFKE+EG VSPLTK+NGD FELTAIAL 
Sbjct: 121 VGDAAFEYGRNRYEEVNVKLSSVMDAVTTCEDGFKEMEGRVSPLTKRNGDVFELTAIALS 180

Query: 181 ILNMRP 187
           ILN+RP
Sbjct: 181 ILNLRP 185

[Close]

BLAST of Lsi05G018940 vs. ExPASy TrEMBL
Match: A0A6J1ITK8 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111479799 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.2e-67
Identity = 139/173 (80.35%), Postives = 148/173 (85.55%), Query Frame = 0


Query: 17  FIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYELGAISINLI 76
           F+     S AAND+V + CEI AA DPNV LDFCLRSLAAAP SD ADLYELGAISI LI
Sbjct: 15  FVALYLNSTAANDVVARNCEIFAAKDPNVHLDFCLRSLAAAPASDMADLYELGAISIELI 74

Query: 77  GRNATSTRRYIERLLKK-KKKLSP--DSYVRPRLSDCKELYSDAVETVGDAAAEYARKRY 136
           GRNATSTRRYIERLLKK KKK +P  DSYVRPRLSDC+ELYSD   TV DAAA+YA KRY
Sbjct: 75  GRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDVKATVRDAAADYASKRY 134

Query: 137 EEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNMRP 187
           EE NVKLSSVMDAVTTCEDGFKE++GGVSPLTK+N DAFELTAIALCILNMRP
Sbjct: 135 EEVNVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCILNMRP 187

[Close]

BLAST of Lsi05G018940 vs. NCBI nr
Match: XP_038905481.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 327.0 bits (837), Expect = 1.0e-85
Identity = 165/186 (88.71%), Postives = 176/186 (94.62%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MSF YTHRFIALA+SF + F+FQS A+NDIVL+TCEISAASDPNVRLDFCLRSLAA P S
Sbjct: 1   MSFTYTHRFIALAYSFLLAFSFQSTASNDIVLRTCEISAASDPNVRLDFCLRSLAADPAS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120
           DTADLYELGAISI LIGRNATSTRRYI+RLLKKKKKLSPDSYVRPRLSDCKELYSDAVET
Sbjct: 61  DTADLYELGAISIELIGRNATSTRRYIDRLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120

Query: 121 VGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALC 180
           VGD++AEYARKRYEE NVKLSSVMDAVTTCEDGFKE+EGG+SPLTK+NGD FELTAIALC
Sbjct: 121 VGDSSAEYARKRYEEVNVKLSSVMDAVTTCEDGFKEMEGGISPLTKRNGDVFELTAIALC 180

Query: 181 ILNMRP 187
           ILNM P
Sbjct: 181 ILNMHP 186

[Close]

BLAST of Lsi05G018940 vs. NCBI nr
Match: XP_008438273.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-76
Identity = 153/187 (81.82%), Postives = 169/187 (90.37%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MSF YTHR+IALAFSF ++ NF+ I+ANDIV +TCE SAA DPNVRLDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRYIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLK-KKKKLSPDSYVRPRLSDCKELYSDAVE 120
           DTADLYELGAISI LIGRNATST+RYIERLLK +KKK S DSY+RPRLSDC+ELYSDAVE
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIAL 180
           TVG+AAAEY RKRY+E NVKLSSVMDAVTTCEDGFKE+E  VSPLTK+NGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 187
           CIL++RP
Sbjct: 181 CILDLRP 187

[Close]

BLAST of Lsi05G018940 vs. NCBI nr
Match: KAA0049108.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK17455.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 295.0 bits (754), Expect = 4.4e-76
Identity = 153/187 (81.82%), Postives = 168/187 (89.84%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MSF YTHR IALAFSF ++ NF+ I+ANDIV +TCE SAA DPNVRLDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRCIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLK-KKKKLSPDSYVRPRLSDCKELYSDAVE 120
           DTADLYELGAISI LIGRNATST+RYIERLLK +KKK S DSY+RPRLSDC+ELYSDAVE
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIAL 180
           TVG+AAAEY RKRY+E NVKLSSVMDAVTTCEDGFKE+E  VSPLTK+NGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 187
           CIL++RP
Sbjct: 181 CILDLRP 187

[Close]

BLAST of Lsi05G018940 vs. NCBI nr
Match: XP_022924291.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 285.4 bits (729), Expect = 3.5e-73
Identity = 150/189 (79.37%), Postives = 161/189 (85.19%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MS  YTHRFIALA S F+     S  AND+V +TCEISAA DPNV LDFCLRSLAAAP S
Sbjct: 1   MSCNYTHRFIALASSLFVALYLTSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPAS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKKKKKL---SPDSYVRPRLSDCKELYSDA 120
           DTADLYELGAISI LIGRNATSTRRYIERLLKKKKK    SPDSY+RP L+DC+ELYSD 
Sbjct: 61  DTADLYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDV 120

Query: 121 VETVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAI 180
             TV DAAA+YA KRYEE NVKLSS+MDAVTTCEDGFKE++GGVSPLTK+N DAFELTAI
Sbjct: 121 RVTVRDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAI 180

Query: 181 ALCILNMRP 187
           ALCILNMRP
Sbjct: 181 ALCILNMRP 189

[Close]

BLAST of Lsi05G018940 vs. NCBI nr
Match: XP_023528439.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284.3 bits (726), Expect = 7.8e-73
Identity = 152/189 (80.42%), Postives = 161/189 (85.19%), Query Frame = 0


Query: 1   MSFAYTHRFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS 60
           MS  YTHRFIALA S F+     S  AND+V +TCEISAA DPNV LDFCLRSLAAAP S
Sbjct: 1   MSCNYTHRFIALASSLFVALYLNSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPAS 60

Query: 61  DTADLYELGAISINLIGRNATSTRRYIERLLKK-KKKLSP--DSYVRPRLSDCKELYSDA 120
           D ADLYELGAISI LIGRNATSTRRYIERLLKK KKK +P  DSYVRPRLSDC+ELYSD 
Sbjct: 61  DMADLYELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDV 120

Query: 121 VETVGDAAAEYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAI 180
             TV DAAA+YA KRYEE NVKLSSVMDAVTTCEDGFKE++GGVSPLTK+N DAFELTAI
Sbjct: 121 KATVRDAAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAI 180

Query: 181 ALCILNMRP 187
           ALCILNMRP
Sbjct: 181 ALCILNMRP 189

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BLAST of Lsi05G018940 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 7.1e-16
Identity = 62/188 (32.98%), Postives = 95/188 (50.53%), Query Frame = 0


Query: 12  LAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVS--DTADLYELG 71
           + F FF+V   +S   +D++ QTC+  AA       +FC+ SL  +P++     +L  L 
Sbjct: 9   IIFLFFLVSQVKS--TSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSSLA 68

Query: 72  AISINLIGRNATSTRRYIERLLKKKKKLSPD--SYVRPRLSDCKELYSDAVETVGDAAAE 131
            + +     NAT+T   I++LL     +S D  S+    L DC ELY DA + + +A   
Sbjct: 69  LVPMLQALDNATATASTIQQLL-----ISDDDGSFRSACLRDCLELYEDATDRLEEAVRV 128

Query: 132 Y-ARKRYEEANVKLSSVMDAVTTCEDGFKEIEGG----------VSPLTKQNGDAFELTA 185
           +  RK     NV +S+ M++  TCE+GF+E + G           SP+  +N   FE   
Sbjct: 129 FITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQ 188

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BLAST of Lsi05G018940 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 7.9e-15
Identity = 52/178 (29.21%), Postives = 91/178 (51.12%), Query Frame = 0


Query: 8   RFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTA-DLY 67
           +F+    +FF++ N  +     ++  +C+ + A+ P  + + C+ SL   P + TA DL 
Sbjct: 2   KFLLYLVTFFVLSNGLA-NGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLA 61

Query: 68  ELGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAA 127
            L   S       AT+ +  +++++K KK    +      L DC +LY+DA+ ++ +A A
Sbjct: 62  GLVMASTKNAVTKATTLKGTVDKIIKGKK---VNKMTAMPLRDCLQLYTDAIGSLNEALA 121

Query: 128 EYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNM 185
               + Y      LS+ MD  +TCE GFKE     SP+TK+N + +++  I L   NM
Sbjct: 122 GVKSRNYPTVKTVLSAAMDTPSTCETGFKE-RKAPSPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of Lsi05G018940 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 8.7e-14
Identity = 53/159 (33.33%), Postives = 84/159 (52.83%), Query Frame = 0


Query: 16  FFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTA-DLYELGAISIN 75
           FF++ N  +I A  ++   C+ +A  +P +  +FC++SL   P S TA  L  L  +S  
Sbjct: 10  FFLLLNAFTI-AQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLSTK 69

Query: 76  LIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAAEYARKRYE 135
                 TS +  ++++LK+ +    + Y    L DC ELYSDA  ++ +A      + Y+
Sbjct: 70  NAVSKTTSMKGIVDKILKENRF---EMYSEKPLRDCLELYSDATNSLKEALTIIKSRDYK 129

Query: 136 EANVKLSSVMDAVTTCEDGFKEIEGGV-SPLTKQNGDAF 173
            ANV +S+ M A  +CE GFKE +  + SP TK N   F
Sbjct: 130 TANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

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BLAST of Lsi05G018940 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.3e-13
Identity = 51/179 (28.49%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 8   RFIALAFSFFIVFNFQSIAANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYE 67
           +F      FF++ N  +  A  ++  +C+ +   DP ++ DFC++SL   P S TA   E
Sbjct: 2   KFSLYLVMFFLLLNCFA-TAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLE 61

Query: 68  -LGAISINLIGRNATSTRRYIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAA 127
            L  +S        TS +  ++++LK+ K       V   L DC ELY++A++++  +  
Sbjct: 62  GLVFVSTKNAVSKTTSLKGMVDKILKEDK-----YEVERPLLDCLELYTEAIDSLNQSLD 121

Query: 128 EYARKRYEEANVKLSSVMDAVTTCEDGFKEIEGGV-SPLTKQNGDAFELTAIALCILNM 185
               + Y+ A + +S+ MDA  +CE  F + +  V SP TK+N   F +  I +   +M
Sbjct: 122 TVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Lsi05G018940 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-11
Identity = 47/159 (29.56%), Postives = 73/159 (45.91%), Query Frame = 0


Query: 27  ANDIVLQTCEISAASDPNVRLDFCLRSLAAAPVSDTADLYE-LGAISINLIGRNATSTRR 86
           A  ++  +C+ + A DP +  DFC+ SL   P S  A   E L   S        T+ + 
Sbjct: 20  AQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTKTAAAKITNLKG 79

Query: 87  YIERLLKKKKKLSPDSYVRPRLSDCKELYSDAVETVGDAAAEYARKRYEEANVKLSSVMD 146
            + + LK ++       +   L  C   Y+DA + +  A A    + Y+ AN+ LS+ +D
Sbjct: 80  IVAQDLKDQRYQD----IVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALD 139

Query: 147 AVTTCEDGFKEIEGGVSPLTKQNGDAFELTAIALCILNM 185
               CED FKE +   SP+T +N   F+   I L   NM
Sbjct: 140 VPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

The following BLAST results are available for this feature:

BLAST of Lsi05G018940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	3.8e-30	43.75	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	1.6e-28	43.93	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	2.6e-10	29.56	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
P83326	2.6e-07	25.27	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LUV1	4.2e-05	23.37	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Lsi05G018940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AVZ7	5.6e-77	81.82	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103483434 PE=4 SV=1	[more]
A0A5A7U1K7	2.1e-76	81.82	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1E955	1.7e-73	79.37	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111431821 PE=4 ...	[more]
A0A0A0L7T4	1.4e-72	78.49	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128870 PE=4 S...	[more]
A0A6J1ITK8	5.2e-67	80.35	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111479799 PE=4 SV...	[more]

BLAST of Lsi05G018940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905481.1	1.0e-85	88.71	putative invertase inhibitor [Benincasa hispida]	[more]
XP_008438273.1	1.2e-76	81.82	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
KAA0049108.1	4.4e-76	81.82	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK17455.1 putative inv...	[more]
XP_022924291.1	3.5e-73	79.37	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_023528439.1	7.8e-73	80.42	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi05G018940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38610.1	7.1e-16	32.98	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	7.9e-15	29.21	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	8.7e-14	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	4.3e-13	28.49	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	1.8e-11	29.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..181 e-value: 3.2E-23 score: 93.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..184 e-value: 2.4E-33 score: 112.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 47..181 e-value: 7.7E-27 score: 94.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..184 e-value: 1.0E-34 score: 121.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 44..184
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 17..183
None	No IPR available	PANTHER	PTHR31080:SF226	PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATED	coord: 17..183
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 35..184 e-value: 2.07921E-42 score: 137.497

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G018940.1	Lsi05G018940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity