Lsi05G018830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G018830
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmetacaspase-1
Locationchr05: 25998778 .. 26002944 (+)
RNA-Seq ExpressionLsi05G018830
SyntenyLsi05G018830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACTATTCGCACCCGCCGCCGATGCTGGTGAACTGCTCCGGCTGCCGGACTCCTCTCCAGCTCCCGCCGGGAGCTCCGTCGATTCGGTGCGCTATATGCAAGGCTGTGACACAGGTTATGGACCCACGTGCCGTCCCTCCGCCGTCGCCTTCTCATGCGCCTCCGCCGGCTCCATCGCCCTACAACCATGCGCCACCAGGACCTCCAGCTCACCCACATGGCCGTAAAAGGGCTGTGATCTGTGGGATCTCTTATAGGTACTCGAGACATGAGCTGAAGGGCTGCCTTAACGATGCTAAGTGTATGCGTTATCTTTTGATCAATAAATTCCATTTTCCAGAAGATTCTATCCTCATGCTTACAGGTACTTTTGCCCAACTCTCTCTCTCTATCTCTGTAGTTCCATCATGAAAATTACTGTGTATTGCTATTTGCTTGTTGATCCTAACTCTCTAATTGTTATGGAAAACACAACTCTCTTCGGCTTCTTGTAATTCTGATGGGCTCATTCTTATAGGAAATGTTTGAATGGCCACTTTTGTTTGTCTTTATATTTGGAAGCATCAGAGATTTCACTTGTGTGTTTTAAGACCTCTAAGGATATATTACCTTACTTGAATAGAAAACAGATTGTACGGACCTATGAGGATATGTTGTTGAGTGTTGGATATAATTTATAGTCATTTTGCAAACATATTACACGGACAGAAGATTAATTGTTAGGAAATGAAATGGAAAATGGTAATCTTATGCTTTTAGGGAGGGTATATTCTGGGTCCAGTTTTAGAACTATGGATATGTAACAAGAAAATGGTAAAACTGTACACTTAGAGGCCGTCACTCTCGACGACTACCTGACTCTAGGAACAGGCTGGGGTGTGTGCTATTTTGTGGTTGCTTTGGGGGAGAAACAACAGAATCTTCAAGGGTTATGAGATTTTATGTCGATGTGTGGTCCTTGTTAGACTCTTGTAAATATTCGCTTGATCTTATTTCTCTTGACTAGAATCTCTTCTTGTAGTTTGTTTTTTATTTATATTTATTTATGTCTTTGTATTCTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTGTTTTCTTGGATGAATGTCATTCTTTTCATCGGGGGACGACCTCCTCCTCCTTCTTGGCTTGGCCCATGTAGCATAGTAGTTATCACGTTGAGTTGAAGAACCCTTTGGAGTTCGTAGTAGAGATCCACTTTTGTGACCAGAATATCTTTGGGAAAATATCAAAGATGATAAGAGGCTTATTTATTTTGAGTCCTATATAAGCAGCAAATCGGATTATTTATTTATTTATTGTTCTTTTTTGGGGGGAATAGGAAACGATAAGTATATTAGAAAAGTCCAAAGCAAGGGCCACAAATGAACGACCTAAGGGTTGGGGTAAAGGAAACCCCTGCCCTTAAATTCACGAATGAAGGCCTTCTAATTAGTTATACTGAGAAGAAGCTGTATGTTTCACACCATTACAACAGAAATCAAAAGGTAATAACGTATCTTCAAATATTATTTGGTTTCTTTATCTCCAATTGAGCCAAAGGAGGCCTTGATTTTAGCCTTGCCTTGGAGATTCCAACCACCTAAAGCTTCAACCATCCAAAATTCTATTTTTTTTGGGAAGAGAAACTTAAATTCCGATATCAAATAAGATTTAGCTGTTCTTATTAAAAGAAAAAAAAAATGTAGGCTGATTTCCAATTTATATTGGAGGGTGATCTCTTTAAATGAATTCTGAGCACATCTCTGCATATCCGTTGTTTTTTTGCAAACTTCCTAAGCTTTTGTGAAATGCAGAAGAAGATTTAGGCTCTATAAGTTATCTCCTCATGCCCTCAATTATCAATCACTCTGATTTATTTCTGTATCTTCTTGTCCCACCACTTGTTTTTACTTATGGTCATTCCGGAAAATGAAATGCAGAAGAAGAAACTGATCCCTACAGAATTCCCTACAAAAACAACATTAGAATGGCATTGTATTGGCTAGTACAAGGTTGTCAACCAGGTGACTCCTTGGTATTCCATTATTCCGGCCACGGTTCTCGCCAAAGGAACTACAACGGTGATGAAGTAGATGGATATGATGAAACTCTTTGCCCTCTGGATTTTGAAACTCAGGGAATGATTATTGATGATGAAATTAATACGGCCATTGTTAGACCTCTTCCTCAAGGCGTAAAGCTTCATGCATTCATAGATGCTTGCCATAGTGGCACCGTACTAGATTTGCCATTTCTATGCAGAATGGGCAGGTTGGTATCTTATAAATATGCTTATGTTTTCTAGCTGAGACATCGATGTTACTCGCACCGAAGTTGATAGTTTTAACTATCCAAAAGAAGATATAGTAGTGATAGAAAAGAAACGGTTAAAAGGTCATTTTGGTCCCTGTACTTTAAGATTTGTTCAATTTTAGTCCATATACTTTCAAATCCAATTTTAATCCCTAAACTTTTAATGAATTTTAAATGTAGTCTCTACTTCTAGTTTATTGTTGATTTTTCAAAAAAACGTTTTATTATCTATTAACATTTTTACTATAAATTTTGGAAACATATTCACATATTATATTTTCTTGCATGAAAATCATTATTATTATTGAATCAATTTTGATTTTAAAAAAAAAAATTCGAGGGACTAAGTTTAAGATTGATTGAAAGTACATGGACTAAAATTGGACATTTGAAAGCATGGGACTAAAATTGAACAAGCTCCAAAATACAAGGACCAAAATGATATTTTAACCAAAATTTCTTTTATGTATCTTCACTTTTCCCCCTGGTTATAGGAGTGGACAATACATGTGGGAGGACCACCGCCCGCGATCCGGAGTATGGAAAGGAACTAGTGGTGGAGAAGCTATTTCTTTCAGTGGCTGTGATGATAACCAAACCTCTGCTGATACATCTGTAAGTTCTATAATCCACAATTCATTTTATTCCATTAGAATTGCTTTATATGACGAAATTATTTGAAGAGATCTGTTCTATAGGTTGTACAATGTCCTTGAGTTCTAAAGAATTGCTTCATATATGAAATGTAATGATGCTTAGCTAATTACTTTAAACGGGAACAGGCTTTATCAAAGATAACATCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCTATCGAGCGAGGACATGGAACTACATATGGAAGCATACTGAACTCTATGCGAAATGCTATAAAAAATGCTGGTGGTAGTGGTGACATTGGCGGTGGCGCTGTGACGTCTTTGGTCACCATGCTTTTGACCGGAGGAAGCGCCCTCGGTGGGCTCAGACAGGTACTTCAATTTTCATGGTAG

mRNA sequence

ATGTACTATTCGCACCCGCCGCCGATGCTGGTGAACTGCTCCGGCTGCCGGACTCCTCTCCAGCTCCCGCCGGGAGCTCCGTCGATTCGGTGCGCTATATGCAAGGCTGTGACACAGGTTATGGACCCACGTGCCGTCCCTCCGCCGTCGCCTTCTCATGCGCCTCCGCCGGCTCCATCGCCCTACAACCATGCGCCACCAGGACCTCCAGCTCACCCACATGGCCGTAAAAGGGCTGTGATCTGTGGGATCTCTTATAGGTACTCGAGACATGAGCTGAAGGGCTGCCTTAACGATGCTAAGTGTATGCGTTATCTTTTGATCAATAAATTCCATTTTCCAGAAGATTCTATCCTCATGCTTACAGAAGAAGAAACTGATCCCTACAGAATTCCCTACAAAAACAACATTAGAATGGCATTGTATTGGCTAGTACAAGGTTGTCAACCAGGTGACTCCTTGGTATTCCATTATTCCGGCCACGGTTCTCGCCAAAGGAACTACAACGGTGATGAAGTAGATGGATATGATGAAACTCTTTGCCCTCTGGATTTTGAAACTCAGGGAATGATTATTGATGATGAAATTAATACGGCCATTGTTAGACCTCTTCCTCAAGGCGTAAAGCTTCATGCATTCATAGATGCTTGCCATAGTGGCACCGTACTAGATTTGCCATTTCTATGCAGAATGGGCAGGTTGGCTTTATCAAAGATAACATCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCTATCGAGCGAGGACATGGAACTACATATGGAAGCATACTGAACTCTATGCGAAATGCTATAAAAAATGCTGGTGGTAGTGGTGACATTGGCGGTGGCGCTGTGACGTCTTTGGTCACCATGCTTTTGACCGGAGGAAGCGCCCTCGGTGGGCTCAGACAGGTACTTCAATTTTCATGGTAG

Coding sequence (CDS)

ATGTACTATTCGCACCCGCCGCCGATGCTGGTGAACTGCTCCGGCTGCCGGACTCCTCTCCAGCTCCCGCCGGGAGCTCCGTCGATTCGGTGCGCTATATGCAAGGCTGTGACACAGGTTATGGACCCACGTGCCGTCCCTCCGCCGTCGCCTTCTCATGCGCCTCCGCCGGCTCCATCGCCCTACAACCATGCGCCACCAGGACCTCCAGCTCACCCACATGGCCGTAAAAGGGCTGTGATCTGTGGGATCTCTTATAGGTACTCGAGACATGAGCTGAAGGGCTGCCTTAACGATGCTAAGTGTATGCGTTATCTTTTGATCAATAAATTCCATTTTCCAGAAGATTCTATCCTCATGCTTACAGAAGAAGAAACTGATCCCTACAGAATTCCCTACAAAAACAACATTAGAATGGCATTGTATTGGCTAGTACAAGGTTGTCAACCAGGTGACTCCTTGGTATTCCATTATTCCGGCCACGGTTCTCGCCAAAGGAACTACAACGGTGATGAAGTAGATGGATATGATGAAACTCTTTGCCCTCTGGATTTTGAAACTCAGGGAATGATTATTGATGATGAAATTAATACGGCCATTGTTAGACCTCTTCCTCAAGGCGTAAAGCTTCATGCATTCATAGATGCTTGCCATAGTGGCACCGTACTAGATTTGCCATTTCTATGCAGAATGGGCAGGTTGGCTTTATCAAAGATAACATCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCTATCGAGCGAGGACATGGAACTACATATGGAAGCATACTGAACTCTATGCGAAATGCTATAAAAAATGCTGGTGGTAGTGGTGACATTGGCGGTGGCGCTGTGACGTCTTTGGTCACCATGCTTTTGACCGGAGGAAGCGCCCTCGGTGGGCTCAGACAGGTACTTCAATTTTCATGGTAG

Protein sequence

MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRLALSKITSTGAMTFCFIQAIERGHGTTYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFSW
Homology
BLAST of Lsi05G018830 vs. ExPASy Swiss-Prot
Match: Q7XJE6 (Metacaspase-1 OS=Arabidopsis thaliana OX=3702 GN=AMC1 PE=1 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 8.9e-133
Identity = 243/346 (70.23%), Postives = 262/346 (75.72%), Query Frame = 0

Query: 5   HPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPSPYNH 64
           + PPMLVNCSGCRTPLQLP GA SIRCA+C+AVT + DPR  PPP PS A  P+P P  H
Sbjct: 10  YAPPMLVNCSGCRTPLQLPSGARSIRCALCQAVTHIADPRTAPPPQPSSA--PSPPPQIH 69

Query: 65  APPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILMLTEE 124
           APPG   HPHGRKRAVICGISYR+SRHELKGC+NDAKCMR+LLINKF F  DSILMLTEE
Sbjct: 70  APPGQLPHPHGRKRAVICGISYRFSRHELKGCINDAKCMRHLLINKFKFSPDSILMLTEE 129

Query: 125 ETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLD 184
           ETDPYRIP K N+RMALYWLVQGC  GDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLD
Sbjct: 130 ETDPYRIPTKQNMRMALYWLVQGCTAGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLD 189

Query: 185 FETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRL---------- 244
           FETQGMI+DDEIN  IVRPLP GVKLH+ IDACHSGTVLDLPFLCRM R           
Sbjct: 190 FETQGMIVDDEINATIVRPLPHGVKLHSIIDACHSGTVLDLPFLCRMNRAGQYVWEDHRP 249

Query: 245 -----------------------------ALSKITSTGAMTFCFIQAIER-GHGTTYGSI 304
                                        ALSKITSTGAMTFCFIQAIER   GTTYGS+
Sbjct: 250 RSGLWKGTAGGEAISISGCDDDQTSADTSALSKITSTGAMTFCFIQAIERSAQGTTYGSL 309

Query: 305 LNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           LNSMR  I+N G  G   GG VT++++MLLTGGSA+GGLRQ  Q +
Sbjct: 310 LNSMRTTIRNTGNDGGGSGGVVTTVLSMLLTGGSAIGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. ExPASy Swiss-Prot
Match: Q7XJE5 (Metacaspase-2 OS=Arabidopsis thaliana OX=3702 GN=AMC2 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.2e-91
Identity = 190/381 (49.87%), Postives = 231/381 (60.63%), Query Frame = 0

Query: 9   MLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVM-DPR----------------------A 68
           +LV+CS CRTPL LPPGA  IRCAIC A T +  +PR                       
Sbjct: 3   LLVDCSSCRTPLHLPPGATRIRCAICHAFTLIAPEPRLQSHASASPFPFPNSSPAPSTFI 62

Query: 69  VPPPSPS-------------HAP---------PPAPSPYNHAPPGPPAHPHGRKRAVICG 128
            PPP+PS             HAP         PPA SP+NHAPPGPP   HG+KRAVI G
Sbjct: 63  YPPPTPSPYTHAPHAPSPFNHAPPDSYPFTHAPPASSPFNHAPPGPPPPVHGQKRAVIVG 122

Query: 129 ISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILMLTEEETDPYRIPYKNNIRMALYW 188
           +SY+ ++ ELKGC+NDA CM+++L+ +F FPE  ILMLTEEE DP R P KNNI MA++W
Sbjct: 123 VSYKNTKDELKGCINDANCMKFMLMKRFQFPESCILMLTEEEADPMRWPTKNNITMAMHW 182

Query: 189 LVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLDFETQGMIIDDEINTAIVRP 248
           LV  C+PGDSLVFH+SGHG+ Q + NGDEVDG+DETL P+D  T G+I+DDEIN  IVRP
Sbjct: 183 LVLSCKPGDSLVFHFSGHGNNQMDDNGDEVDGFDETLLPVDHRTSGVIVDDEINATIVRP 242

Query: 249 LPQGVKLHAFIDACHSGTVLDLPFLCRMGRLA---------------------------- 299
           LP GVKLHA +DACHSGTV+DLP+LCRM RL                             
Sbjct: 243 LPYGVKLHAIVDACHSGTVMDLPYLCRMDRLGNYEWEDHRPKTGMWKGTSGGEVFSFTGC 302

BLAST of Lsi05G018830 vs. ExPASy Swiss-Prot
Match: Q9FMG1 (Metacaspase-3 OS=Arabidopsis thaliana OX=3702 GN=AMC3 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-60
Identity = 141/313 (45.05%), Postives = 174/313 (55.59%), Query Frame = 0

Query: 6   PPPMLVNCSGCRTPLQLPPGAPSIRCA--ICKAVTQVMDPRAVPPPSPSHAPPPAPSPYN 65
           P    V CS C T  QL       R A  I     Q++         P H          
Sbjct: 19  PDARTVQCSTCHTVTQLYSLVDIARGANRIIHGFQQLLRQH-----QPQHHEQQQQQMMA 78

Query: 66  HAPP---GPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 125
             PP    P   P G+KRAV+CG++Y+   + LKGC++DAK MR LL+ +  FP DSILM
Sbjct: 79  QPPPRLLEPLPSPFGKKRAVLCGVNYKGKSYSLKGCISDAKSMRSLLVQQMGFPIDSILM 138

Query: 126 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 185
           LTE+E  P RIP K NIR A+ WLV+G +  DSLVFH+SGHGS+Q +YNGDE+DG DE L
Sbjct: 139 LTEDEASPQRIPTKRNIRKAMRWLVEGNRARDSLVFHFSGHGSQQNDYNGDEIDGQDEAL 198

Query: 186 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRM--------- 245
           CPLD ET+G IIDDEIN  +VRPL  G KLHA IDAC+SGTVLDLPF+CRM         
Sbjct: 199 CPLDHETEGKIIDDEINRILVRPLVHGAKLHAVIDACNSGTVLDLPFICRMERNGSYEWE 258

Query: 246 ----------------------------GRLALSKITSTGAMTFCFIQAIE-RGHGTTYG 276
                                       G   +    +TGAMT+ FI+A++  G   TYG
Sbjct: 259 DHRSVRAYKGTDGGAAFCFSACDDDESSGYTPVFTGKNTGAMTYSFIKAVKTAGPAPTYG 318

BLAST of Lsi05G018830 vs. ExPASy Swiss-Prot
Match: Q75B43 (Metacaspase-1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=MCA1 PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.9e-42
Identity = 85/179 (47.49%), Postives = 122/179 (68.16%), Query Frame = 0

Query: 54  APPPAP----SPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLIN 113
           APPP       P N +     +   G ++A++ GI+Y  S  EL+GC+ND + ++  LI+
Sbjct: 127 APPPQERQFLDPGNRSVEYQYSQCTGNRKALLIGINYFNSSAELRGCINDVQNIKNFLIS 186

Query: 114 KFHFPEDSILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYN 173
           ++ + E+++++LT+++ DP RIP K NI  A++WLVQG QP DSL  HYSGHG    + +
Sbjct: 187 RYGYREENMVILTDDQHDPVRIPTKANILRAMHWLVQGAQPNDSLFLHYSGHGGETEDLD 246

Query: 174 GDEVDGYDETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFL 229
           GDE DG D TL P+DFET G I+DDEI+  +V+PL  GV+L A IDACHSG+ LDLP++
Sbjct: 247 GDEQDGKDSTLYPVDFETNGHIVDDEIHDILVKPLAPGVRLTALIDACHSGSALDLPYM 305

BLAST of Lsi05G018830 vs. ExPASy Swiss-Prot
Match: Q1E0A3 (Metacaspase-1 OS=Coccidioides immitis (strain RS) OX=246410 GN=MCA1 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.8e-40
Identity = 79/182 (43.41%), Postives = 120/182 (65.93%), Query Frame = 0

Query: 54  APPPAPS---PYNHAPPGPPAHPH----GRKRAVICGISYRYSRHELKGCLNDAKCMRYL 113
           APPP PS    + H  P   ++ +    G+++A++ GI+Y   R +L+GC+ND K M   
Sbjct: 139 APPPPPSGSVAFGHGAPQGYSYQYSNCTGKRKALLIGINYFGQRGQLRGCINDVKNMSTY 198

Query: 114 LINKFHFPEDSILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQR 173
           L   F++  + +++LT+++ +P   P K NI  A++WLV+  +P DSL FHYSGHG +  
Sbjct: 199 LNQSFNYAREDMVILTDDQQNPMSQPTKANILRAMHWLVKDARPNDSLFFHYSGHGGQTP 258

Query: 174 NYNGDEVDGYDETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLP 229
           + +GDE DGYDE + P+DF   G I+DDE++  +VRPLP GV+L A  D+CHSG+ LDLP
Sbjct: 259 DLDGDEDDGYDEVIYPVDFRNAGHIVDDEMHRIMVRPLPAGVRLTAIFDSCHSGSALDLP 318

BLAST of Lsi05G018830 vs. ExPASy TrEMBL
Match: A0A6J1IZ01 (metacaspase-1 OS=Cucurbita maxima OX=3661 GN=LOC111479732 PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 5.7e-167
Identity = 297/349 (85.10%), Postives = 302/349 (86.53%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPS 60
           MYY HPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVP PSPSHAPPPAPS
Sbjct: 1   MYYPHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPSPSPSHAPPPAPS 60

Query: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 120
           PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLL+NKF FPEDSILM
Sbjct: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLVNKFRFPEDSILM 120

Query: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180
           LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL
Sbjct: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180

Query: 181 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR------- 240
           CPLDFETQGMI+DDEINTAIV+PLPQGVKLHAFIDACHSGTVLDLPFLCRM R       
Sbjct: 181 CPLDFETQGMIVDDEINTAIVKPLPQGVKLHAFIDACHSGTVLDLPFLCRMRRSGQYMWE 240

Query: 241 --------------------------------LALSKITSTGAMTFCFIQAIERGHGTTY 300
                                            ALSKITSTGAMTFCFIQAIERGHGTTY
Sbjct: 241 DHRPRSGVWKGTSGGEAISFSGCDDNETSADTSALSKITSTGAMTFCFIQAIERGHGTTY 300

Query: 301 GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSA+GGLRQ  Q +
Sbjct: 301 GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSAIGGLRQEPQLT 349

BLAST of Lsi05G018830 vs. ExPASy TrEMBL
Match: A0A6J1EFN1 (metacaspase-1 OS=Cucurbita moschata OX=3662 GN=LOC111432096 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.3e-166
Identity = 295/349 (84.53%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPS 60
           MYY HPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRA P PSPSHAPPPAPS
Sbjct: 1   MYYPHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAAPSPSPSHAPPPAPS 60

Query: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 120
           PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLL+NKF FPEDSILM
Sbjct: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLVNKFRFPEDSILM 120

Query: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180
           LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL
Sbjct: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180

Query: 181 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR------- 240
           CPLDFETQGMI+DDEINTAIV+PLPQGVKLHAFIDACHSGTVLDLPFLCRM R       
Sbjct: 181 CPLDFETQGMIVDDEINTAIVKPLPQGVKLHAFIDACHSGTVLDLPFLCRMRRSGQYMWE 240

Query: 241 --------------------------------LALSKITSTGAMTFCFIQAIERGHGTTY 300
                                            ALSKITSTGAMTFCFIQAIERGHGTTY
Sbjct: 241 DHRPRSGVWKGTSGGEAISFSGCDDNETSADTSALSKITSTGAMTFCFIQAIERGHGTTY 300

Query: 301 GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GSILNSMRNAIK+AGGSGDIGGGAVTSLVTMLLTGGSA+GGLRQ  Q +
Sbjct: 301 GSILNSMRNAIKSAGGSGDIGGGAVTSLVTMLLTGGSAIGGLRQEPQLT 349

BLAST of Lsi05G018830 vs. ExPASy TrEMBL
Match: A0A5D3D0F3 (Metacaspase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003110 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.4e-165
Identity = 297/353 (84.14%), Postives = 302/353 (85.55%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPP---- 60
           MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVT VMDPRAVPPPSPS APP    
Sbjct: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTHVMDPRAVPPPSPSQAPPPASA 60

Query: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPED 120
           PAPSPYNHAPPGPPAHPHGRKRAVICG+SYRYSRHELKGCLNDAKCMRYLLINKF FPED
Sbjct: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGVSYRYSRHELKGCLNDAKCMRYLLINKFRFPED 120

Query: 121 SILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180
           SILMLTEEETDPYRIPYKNNIRMAL+WLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY
Sbjct: 121 SILMLTEEETDPYRIPYKNNIRMALFWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180

Query: 181 DETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR--- 240
           DETLCPLDFETQGMI+DDEIN AIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR   
Sbjct: 181 DETLCPLDFETQGMIVDDEINAAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRSGQ 240

Query: 241 ------------------------------------LALSKITSTGAMTFCFIQAIERGH 300
                                                ALSKITSTGAMTFCFIQAIERGH
Sbjct: 241 YMWEDHRPRSGVWKGTSGGEAISFSGCDDNQTSADTSALSKITSTGAMTFCFIQAIERGH 300

Query: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GTTYGSILNSMRNAIKNAGGSGDIGGGA+TSLVTMLLTGGSALGGLRQ  Q +
Sbjct: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAMTSLVTMLLTGGSALGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. ExPASy TrEMBL
Match: A0A1S3AWN8 (metacaspase-1 OS=Cucumis melo OX=3656 GN=LOC103483445 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.4e-165
Identity = 297/353 (84.14%), Postives = 302/353 (85.55%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPP---- 60
           MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVT VMDPRAVPPPSPS APP    
Sbjct: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTHVMDPRAVPPPSPSQAPPPASA 60

Query: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPED 120
           PAPSPYNHAPPGPPAHPHGRKRAVICG+SYRYSRHELKGCLNDAKCMRYLLINKF FPED
Sbjct: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGVSYRYSRHELKGCLNDAKCMRYLLINKFRFPED 120

Query: 121 SILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180
           SILMLTEEETDPYRIPYKNNIRMAL+WLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY
Sbjct: 121 SILMLTEEETDPYRIPYKNNIRMALFWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180

Query: 181 DETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR--- 240
           DETLCPLDFETQGMI+DDEIN AIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR   
Sbjct: 181 DETLCPLDFETQGMIVDDEINAAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRSGQ 240

Query: 241 ------------------------------------LALSKITSTGAMTFCFIQAIERGH 300
                                                ALSKITSTGAMTFCFIQAIERGH
Sbjct: 241 YMWEDHRPRSGVWKGTSGGEAISFSGCDDNQTSADTSALSKITSTGAMTFCFIQAIERGH 300

Query: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GTTYGSILNSMRNAIKNAGGSGDIGGGA+TSLVTMLLTGGSALGGLRQ  Q +
Sbjct: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAMTSLVTMLLTGGSALGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. ExPASy TrEMBL
Match: A0A0A0L401 (zf-LSD1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129470 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 293/353 (83.00%), Postives = 300/353 (84.99%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSH----APP 60
           MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPS     AP 
Sbjct: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSQAASPAPA 60

Query: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPED 120
           PAPSPYNHAPPG PAHPHGRKRAVICG+SYRYSRHELKGCLNDAKCMRYLLINKF FPED
Sbjct: 61  PAPSPYNHAPPGAPAHPHGRKRAVICGVSYRYSRHELKGCLNDAKCMRYLLINKFRFPED 120

Query: 121 SILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180
           SILMLTEEETDPYRIPYKNNIRMAL+WLVQGCQPGDSLVFHYSGHGS QRNYNGDEVDGY
Sbjct: 121 SILMLTEEETDPYRIPYKNNIRMALFWLVQGCQPGDSLVFHYSGHGSHQRNYNGDEVDGY 180

Query: 181 DETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR--- 240
           DETLCPLDFETQGMI+DDEIN AIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR   
Sbjct: 181 DETLCPLDFETQGMIVDDEINAAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRSGQ 240

Query: 241 ------------------------------------LALSKITSTGAMTFCFIQAIERGH 300
                                                ALSKITSTGAMTFCFIQAIERGH
Sbjct: 241 YMWEDHRPRSGVWKGTSGGEAISFSGCDDNETSADTSALSKITSTGAMTFCFIQAIERGH 300

Query: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GTTYGSILNSMRNAI+NAGGSGDIGGGA+TSLVTMLL+GGSALGGLRQ  Q +
Sbjct: 301 GTTYGSILNSMRNAIRNAGGSGDIGGGAMTSLVTMLLSGGSALGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. NCBI nr
Match: XP_022980333.1 (metacaspase-1 [Cucurbita maxima] >XP_023528372.1 metacaspase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596.7 bits (1537), Expect = 1.2e-166
Identity = 297/349 (85.10%), Postives = 302/349 (86.53%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPS 60
           MYY HPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVP PSPSHAPPPAPS
Sbjct: 1   MYYPHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPSPSPSHAPPPAPS 60

Query: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 120
           PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLL+NKF FPEDSILM
Sbjct: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLVNKFRFPEDSILM 120

Query: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180
           LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL
Sbjct: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180

Query: 181 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR------- 240
           CPLDFETQGMI+DDEINTAIV+PLPQGVKLHAFIDACHSGTVLDLPFLCRM R       
Sbjct: 181 CPLDFETQGMIVDDEINTAIVKPLPQGVKLHAFIDACHSGTVLDLPFLCRMRRSGQYMWE 240

Query: 241 --------------------------------LALSKITSTGAMTFCFIQAIERGHGTTY 300
                                            ALSKITSTGAMTFCFIQAIERGHGTTY
Sbjct: 241 DHRPRSGVWKGTSGGEAISFSGCDDNETSADTSALSKITSTGAMTFCFIQAIERGHGTTY 300

Query: 301 GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSA+GGLRQ  Q +
Sbjct: 301 GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSAIGGLRQEPQLT 349

BLAST of Lsi05G018830 vs. NCBI nr
Match: XP_022924660.1 (metacaspase-1 [Cucurbita moschata] >KAG6582507.1 Metacaspase-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 295/349 (84.53%), Postives = 301/349 (86.25%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPS 60
           MYY HPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRA P PSPSHAPPPAPS
Sbjct: 1   MYYPHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAAPSPSPSHAPPPAPS 60

Query: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 120
           PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLL+NKF FPEDSILM
Sbjct: 61  PYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLVNKFRFPEDSILM 120

Query: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180
           LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL
Sbjct: 121 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 180

Query: 181 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR------- 240
           CPLDFETQGMI+DDEINTAIV+PLPQGVKLHAFIDACHSGTVLDLPFLCRM R       
Sbjct: 181 CPLDFETQGMIVDDEINTAIVKPLPQGVKLHAFIDACHSGTVLDLPFLCRMRRSGQYMWE 240

Query: 241 --------------------------------LALSKITSTGAMTFCFIQAIERGHGTTY 300
                                            ALSKITSTGAMTFCFIQAIERGHGTTY
Sbjct: 241 DHRPRSGVWKGTSGGEAISFSGCDDNETSADTSALSKITSTGAMTFCFIQAIERGHGTTY 300

Query: 301 GSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GSILNSMRNAIK+AGGSGDIGGGAVTSLVTMLLTGGSA+GGLRQ  Q +
Sbjct: 301 GSILNSMRNAIKSAGGSGDIGGGAVTSLVTMLLTGGSAIGGLRQEPQLT 349

BLAST of Lsi05G018830 vs. NCBI nr
Match: XP_008438286.1 (PREDICTED: metacaspase-1 [Cucumis melo] >KAA0049117.1 metacaspase-1-like [Cucumis melo var. makuwa] >TYK17447.1 metacaspase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 591.3 bits (1523), Expect = 5.0e-165
Identity = 297/353 (84.14%), Postives = 302/353 (85.55%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPP---- 60
           MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVT VMDPRAVPPPSPS APP    
Sbjct: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTHVMDPRAVPPPSPSQAPPPASA 60

Query: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPED 120
           PAPSPYNHAPPGPPAHPHGRKRAVICG+SYRYSRHELKGCLNDAKCMRYLLINKF FPED
Sbjct: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGVSYRYSRHELKGCLNDAKCMRYLLINKFRFPED 120

Query: 121 SILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180
           SILMLTEEETDPYRIPYKNNIRMAL+WLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY
Sbjct: 121 SILMLTEEETDPYRIPYKNNIRMALFWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180

Query: 181 DETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR--- 240
           DETLCPLDFETQGMI+DDEIN AIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR   
Sbjct: 181 DETLCPLDFETQGMIVDDEINAAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRSGQ 240

Query: 241 ------------------------------------LALSKITSTGAMTFCFIQAIERGH 300
                                                ALSKITSTGAMTFCFIQAIERGH
Sbjct: 241 YMWEDHRPRSGVWKGTSGGEAISFSGCDDNQTSADTSALSKITSTGAMTFCFIQAIERGH 300

Query: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GTTYGSILNSMRNAIKNAGGSGDIGGGA+TSLVTMLLTGGSALGGLRQ  Q +
Sbjct: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAMTSLVTMLLTGGSALGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. NCBI nr
Match: XP_038881686.1 (metacaspase-1 [Benincasa hispida])

HSP 1 Score: 584.7 bits (1506), Expect = 4.6e-163
Identity = 293/351 (83.48%), Postives = 301/351 (85.75%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPA-- 60
           MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAIC+ VTQVMDPRAVPPPSPSH  PPA  
Sbjct: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICRTVTQVMDPRAVPPPSPSHTTPPAPV 60

Query: 61  PSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSI 120
           PSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSI
Sbjct: 61  PSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSI 120

Query: 121 LMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDE 180
           LMLTEEETDPYRIPYK+NIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDE
Sbjct: 121 LMLTEEETDPYRIPYKSNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDE 180

Query: 181 TLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR----- 240
           TLCPLDFETQGMI+DDEIN+AIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR     
Sbjct: 181 TLCPLDFETQGMIVDDEINSAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRSGQYM 240

Query: 241 ----------------------------------LALSKITSTGAMTFCFIQAIERGHGT 300
                                              ALSKITSTGAMTFCFIQAIERGHGT
Sbjct: 241 WEDHRPRSGVWKGTSGGEAISFSGCDDDQTSADTSALSKITSTGAMTFCFIQAIERGHGT 300

Query: 301 TYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           TYGSILNSMRNAIKNAGGSGDI GG VTSLV+MLL+GGSA+GGLRQ  Q +
Sbjct: 301 TYGSILNSMRNAIKNAGGSGDIIGGTVTSLVSMLLSGGSAVGGLRQEPQLT 351

BLAST of Lsi05G018830 vs. NCBI nr
Match: XP_004133968.2 (metacaspase-1 [Cucumis sativus] >KAE8650291.1 hypothetical protein Csa_009465 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 2.3e-162
Identity = 293/353 (83.00%), Postives = 300/353 (84.99%), Query Frame = 0

Query: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSH----APP 60
           MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPS     AP 
Sbjct: 1   MYYSHPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSQAASPAPA 60

Query: 61  PAPSPYNHAPPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPED 120
           PAPSPYNHAPPG PAHPHGRKRAVICG+SYRYSRHELKGCLNDAKCMRYLLINKF FPED
Sbjct: 61  PAPSPYNHAPPGAPAHPHGRKRAVICGVSYRYSRHELKGCLNDAKCMRYLLINKFRFPED 120

Query: 121 SILMLTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGY 180
           SILMLTEEETDPYRIPYKNNIRMAL+WLVQGCQPGDSLVFHYSGHGS QRNYNGDEVDGY
Sbjct: 121 SILMLTEEETDPYRIPYKNNIRMALFWLVQGCQPGDSLVFHYSGHGSHQRNYNGDEVDGY 180

Query: 181 DETLCPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR--- 240
           DETLCPLDFETQGMI+DDEIN AIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR   
Sbjct: 181 DETLCPLDFETQGMIVDDEINAAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRSGQ 240

Query: 241 ------------------------------------LALSKITSTGAMTFCFIQAIERGH 300
                                                ALSKITSTGAMTFCFIQAIERGH
Sbjct: 241 YMWEDHRPRSGVWKGTSGGEAISFSGCDDNETSADTSALSKITSTGAMTFCFIQAIERGH 300

Query: 301 GTTYGSILNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           GTTYGSILNSMRNAI+NAGGSGDIGGGA+TSLVTMLL+GGSALGGLRQ  Q +
Sbjct: 301 GTTYGSILNSMRNAIRNAGGSGDIGGGAMTSLVTMLLSGGSALGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. TAIR 10
Match: AT1G02170.1 (metacaspase 1 )

HSP 1 Score: 474.6 bits (1220), Expect = 6.3e-134
Identity = 243/346 (70.23%), Postives = 262/346 (75.72%), Query Frame = 0

Query: 5   HPPPMLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVMDPRAVPPPSPSHAPPPAPSPYNH 64
           + PPMLVNCSGCRTPLQLP GA SIRCA+C+AVT + DPR  PPP PS A  P+P P  H
Sbjct: 10  YAPPMLVNCSGCRTPLQLPSGARSIRCALCQAVTHIADPRTAPPPQPSSA--PSPPPQIH 69

Query: 65  APPGPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILMLTEE 124
           APPG   HPHGRKRAVICGISYR+SRHELKGC+NDAKCMR+LLINKF F  DSILMLTEE
Sbjct: 70  APPGQLPHPHGRKRAVICGISYRFSRHELKGCINDAKCMRHLLINKFKFSPDSILMLTEE 129

Query: 125 ETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLD 184
           ETDPYRIP K N+RMALYWLVQGC  GDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLD
Sbjct: 130 ETDPYRIPTKQNMRMALYWLVQGCTAGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLD 189

Query: 185 FETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGRL---------- 244
           FETQGMI+DDEIN  IVRPLP GVKLH+ IDACHSGTVLDLPFLCRM R           
Sbjct: 190 FETQGMIVDDEINATIVRPLPHGVKLHSIIDACHSGTVLDLPFLCRMNRAGQYVWEDHRP 249

Query: 245 -----------------------------ALSKITSTGAMTFCFIQAIER-GHGTTYGSI 304
                                        ALSKITSTGAMTFCFIQAIER   GTTYGS+
Sbjct: 250 RSGLWKGTAGGEAISISGCDDDQTSADTSALSKITSTGAMTFCFIQAIERSAQGTTYGSL 309

Query: 305 LNSMRNAIKNAGGSGDIGGGAVTSLVTMLLTGGSALGGLRQVLQFS 311
           LNSMR  I+N G  G   GG VT++++MLLTGGSA+GGLRQ  Q +
Sbjct: 310 LNSMRTTIRNTGNDGGGSGGVVTTVLSMLLTGGSAIGGLRQEPQLT 353

BLAST of Lsi05G018830 vs. TAIR 10
Match: AT4G25110.1 (metacaspase 2 )

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-92
Identity = 190/381 (49.87%), Postives = 231/381 (60.63%), Query Frame = 0

Query: 9   MLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVM-DPR----------------------A 68
           +LV+CS CRTPL LPPGA  IRCAIC A T +  +PR                       
Sbjct: 3   LLVDCSSCRTPLHLPPGATRIRCAICHAFTLIAPEPRLQSHASASPFPFPNSSPAPSTFI 62

Query: 69  VPPPSPS-------------HAP---------PPAPSPYNHAPPGPPAHPHGRKRAVICG 128
            PPP+PS             HAP         PPA SP+NHAPPGPP   HG+KRAVI G
Sbjct: 63  YPPPTPSPYTHAPHAPSPFNHAPPDSYPFTHAPPASSPFNHAPPGPPPPVHGQKRAVIVG 122

Query: 129 ISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILMLTEEETDPYRIPYKNNIRMALYW 188
           +SY+ ++ ELKGC+NDA CM+++L+ +F FPE  ILMLTEEE DP R P KNNI MA++W
Sbjct: 123 VSYKNTKDELKGCINDANCMKFMLMKRFQFPESCILMLTEEEADPMRWPTKNNITMAMHW 182

Query: 189 LVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLDFETQGMIIDDEINTAIVRP 248
           LV  C+PGDSLVFH+SGHG+ Q + NGDEVDG+DETL P+D  T G+I+DDEIN  IVRP
Sbjct: 183 LVLSCKPGDSLVFHFSGHGNNQMDDNGDEVDGFDETLLPVDHRTSGVIVDDEINATIVRP 242

Query: 249 LPQGVKLHAFIDACHSGTVLDLPFLCRMGRLA---------------------------- 299
           LP GVKLHA +DACHSGTV+DLP+LCRM RL                             
Sbjct: 243 LPYGVKLHAIVDACHSGTVMDLPYLCRMDRLGNYEWEDHRPKTGMWKGTSGGEVFSFTGC 302

BLAST of Lsi05G018830 vs. TAIR 10
Match: AT4G25110.2 (metacaspase 2 )

HSP 1 Score: 330.5 bits (846), Expect = 1.5e-90
Identity = 189/381 (49.61%), Postives = 230/381 (60.37%), Query Frame = 0

Query: 9   MLVNCSGCRTPLQLPPGAPSIRCAICKAVTQVM-DPR----------------------A 68
           +LV+CS CRTPL LPPGA  IRCAIC A T +  +PR                       
Sbjct: 3   LLVDCSSCRTPLHLPPGATRIRCAICHAFTLIAPEPRLQSHASASPFPFPNSSPAPSTFI 62

Query: 69  VPPPSPS-------------HAP---------PPAPSPYNHAPPGPPAHPHGRKRAVICG 128
            PPP+PS             HAP         PPA SP+NHAPPGPP   HG+KRAVI G
Sbjct: 63  YPPPTPSPYTHAPHAPSPFNHAPPDSYPFTHAPPASSPFNHAPPGPPPPVHGQKRAVIVG 122

Query: 129 ISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILMLTEEETDPYRIPYKNNIRMALYW 188
           +SY+ ++ ELKGC+NDA CM+++L+ +F FPE  ILMLT EE DP R P KNNI MA++W
Sbjct: 123 VSYKNTKDELKGCINDANCMKFMLMKRFQFPESCILMLT-EEADPMRWPTKNNITMAMHW 182

Query: 189 LVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETLCPLDFETQGMIIDDEINTAIVRP 248
           LV  C+PGDSLVFH+SGHG+ Q + NGDEVDG+DETL P+D  T G+I+DDEIN  IVRP
Sbjct: 183 LVLSCKPGDSLVFHFSGHGNNQMDDNGDEVDGFDETLLPVDHRTSGVIVDDEINATIVRP 242

Query: 249 LPQGVKLHAFIDACHSGTVLDLPFLCRMGRLA---------------------------- 299
           LP GVKLHA +DACHSGTV+DLP+LCRM RL                             
Sbjct: 243 LPYGVKLHAIVDACHSGTVMDLPYLCRMDRLGNYEWEDHRPKTGMWKGTSGGEVFSFTGC 302

BLAST of Lsi05G018830 vs. TAIR 10
Match: AT5G64240.2 (metacaspase 3 )

HSP 1 Score: 235.0 bits (598), Expect = 8.4e-62
Identity = 141/313 (45.05%), Postives = 174/313 (55.59%), Query Frame = 0

Query: 6   PPPMLVNCSGCRTPLQLPPGAPSIRCA--ICKAVTQVMDPRAVPPPSPSHAPPPAPSPYN 65
           P    V CS C T  QL       R A  I     Q++         P H          
Sbjct: 19  PDARTVQCSTCHTVTQLYSLVDIARGANRIIHGFQQLLRQH-----QPQHHEQQQQQMMA 78

Query: 66  HAPP---GPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 125
             PP    P   P G+KRAV+CG++Y+   + LKGC++DAK MR LL+ +  FP DSILM
Sbjct: 79  QPPPRLLEPLPSPFGKKRAVLCGVNYKGKSYSLKGCISDAKSMRSLLVQQMGFPIDSILM 138

Query: 126 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 185
           LTE+E  P RIP K NIR A+ WLV+G +  DSLVFH+SGHGS+Q +YNGDE+DG DE L
Sbjct: 139 LTEDEASPQRIPTKRNIRKAMRWLVEGNRARDSLVFHFSGHGSQQNDYNGDEIDGQDEAL 198

Query: 186 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRM--------- 245
           CPLD ET+G IIDDEIN  +VRPL  G KLHA IDAC+SGTVLDLPF+CRM         
Sbjct: 199 CPLDHETEGKIIDDEINRILVRPLVHGAKLHAVIDACNSGTVLDLPFICRMERNGSYEWE 258

Query: 246 ----------------------------GRLALSKITSTGAMTFCFIQAIE-RGHGTTYG 276
                                       G   +    +TGAMT+ FI+A++  G   TYG
Sbjct: 259 DHRSVRAYKGTDGGAAFCFSACDDDESSGYTPVFTGKNTGAMTYSFIKAVKTAGPAPTYG 318

BLAST of Lsi05G018830 vs. TAIR 10
Match: AT5G64240.1 (metacaspase 3 )

HSP 1 Score: 225.3 bits (573), Expect = 6.7e-59
Identity = 123/233 (52.79%), Postives = 147/233 (63.09%), Query Frame = 0

Query: 6   PPPMLVNCSGCRTPLQLPPGAPSIRCA--ICKAVTQVMDPRAVPPPSPSHAPPPAPSPYN 65
           P    V CS C T  QL       R A  I     Q++         P H          
Sbjct: 19  PDARTVQCSTCHTVTQLYSLVDIARGANRIIHGFQQLLRQH-----QPQHHEQQQQQMMA 78

Query: 66  HAPP---GPPAHPHGRKRAVICGISYRYSRHELKGCLNDAKCMRYLLINKFHFPEDSILM 125
             PP    P   P G+KRAV+CG++Y+   + LKGC++DAK MR LL+ +  FP DSILM
Sbjct: 79  QPPPRLLEPLPSPFGKKRAVLCGVNYKGKSYSLKGCISDAKSMRSLLVQQMGFPIDSILM 138

Query: 126 LTEEETDPYRIPYKNNIRMALYWLVQGCQPGDSLVFHYSGHGSRQRNYNGDEVDGYDETL 185
           LTE+E  P RIP K NIR A+ WLV+G +  DSLVFH+SGHGS+Q +YNGDE+DG DE L
Sbjct: 139 LTEDEASPQRIPTKRNIRKAMRWLVEGNRARDSLVFHFSGHGSQQNDYNGDEIDGQDEAL 198

Query: 186 CPLDFETQGMIIDDEINTAIVRPLPQGVKLHAFIDACHSGTVLDLPFLCRMGR 234
           CPLD ET+G IIDDEIN  +VRPL  G KLHA IDAC+SGTVLDLPF+CRM R
Sbjct: 199 CPLDHETEGKIIDDEINRILVRPLVHGAKLHAVIDACNSGTVLDLPFICRMER 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XJE68.9e-13370.23Metacaspase-1 OS=Arabidopsis thaliana OX=3702 GN=AMC1 PE=1 SV=1[more]
Q7XJE52.2e-9149.87Metacaspase-2 OS=Arabidopsis thaliana OX=3702 GN=AMC2 PE=1 SV=1[more]
Q9FMG11.2e-6045.05Metacaspase-3 OS=Arabidopsis thaliana OX=3702 GN=AMC3 PE=2 SV=1[more]
Q75B431.9e-4247.49Metacaspase-1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / N... [more]
Q1E0A38.8e-4043.41Metacaspase-1 OS=Coccidioides immitis (strain RS) OX=246410 GN=MCA1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IZ015.7e-16785.10metacaspase-1 OS=Cucurbita maxima OX=3661 GN=LOC111479732 PE=4 SV=1[more]
A0A6J1EFN16.3e-16684.53metacaspase-1 OS=Cucurbita moschata OX=3662 GN=LOC111432096 PE=4 SV=1[more]
A0A5D3D0F32.4e-16584.14Metacaspase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003110... [more]
A0A1S3AWN82.4e-16584.14metacaspase-1 OS=Cucumis melo OX=3656 GN=LOC103483445 PE=4 SV=1[more]
A0A0A0L4011.1e-16283.00zf-LSD1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129470 PE=... [more]
Match NameE-valueIdentityDescription
XP_022980333.11.2e-16685.10metacaspase-1 [Cucurbita maxima] >XP_023528372.1 metacaspase-1 [Cucurbita pepo s... [more]
XP_022924660.11.3e-16584.53metacaspase-1 [Cucurbita moschata] >KAG6582507.1 Metacaspase-1, partial [Cucurbi... [more]
XP_008438286.15.0e-16584.14PREDICTED: metacaspase-1 [Cucumis melo] >KAA0049117.1 metacaspase-1-like [Cucumi... [more]
XP_038881686.14.6e-16383.48metacaspase-1 [Benincasa hispida][more]
XP_004133968.22.3e-16283.00metacaspase-1 [Cucumis sativus] >KAE8650291.1 hypothetical protein Csa_009465 [C... [more]
Match NameE-valueIdentityDescription
AT1G02170.16.3e-13470.23metacaspase 1 [more]
AT4G25110.11.6e-9249.87metacaspase 2 [more]
AT4G25110.21.5e-9049.61metacaspase 2 [more]
AT5G64240.28.4e-6245.05metacaspase 3 [more]
AT5G64240.16.7e-5952.79metacaspase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005735Zinc finger, LSD1-typePFAMPF06943zf-LSD1coord: 13..37
e-value: 1.1E-11
score: 44.4
IPR005735Zinc finger, LSD1-typeTIGRFAMTIGR01053TIGR01053coord: 10..40
e-value: 5.3E-16
score: 55.9
NoneNo IPR availablePFAMPF00656Peptidase_C14coord: 77..235
e-value: 3.1E-41
score: 142.0
NoneNo IPR availableGENE3D3.40.50.12660coord: 29..278
e-value: 2.8E-83
score: 282.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..74
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..73
NoneNo IPR availablePANTHERPTHR48104METACASPASE-4coord: 9..233
coord: 234..309
IPR033180Metacaspase type I, plantPANTHERPTHR48104:SF32METACASPASE-1-LIKEcoord: 9..233
coord: 234..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G018830.1Lsi05G018830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043068 positive regulation of programmed cell death
biological_process GO:0006508 proteolysis
molecular_function GO:0004197 cysteine-type endopeptidase activity