Lsi05G017580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G017580
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein NRT1/ PTR FAMILY 5.2-like
Locationchr05: 24961181 .. 24966102 (+)
RNA-Seq ExpressionLsi05G017580
SyntenyLsi05G017580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATATATGCCACATGGCAAAGTTTTTGATTGATATAGAGTATCAGCTGCATCCAAGTGTTTTTACAAAAGGGCAGTGGTGTCTCATATTTCATAAACAAATACCCTTTGTGTTGGAGCTGCTTCATAGAAGTAATAAAAAGAGAGAGAAATAGGGAAAAGGAAAATCATCATCATCATCATAATAATAATAGTACTTCCTCCGCCATTAATGGCGGGTGCTGCAGCAGATCAAGAAACCGGGCTCGACGATTACACCAAAGATGGAACCGTGGATCGGAAAGGCAACCCGGTTCTCCGCTCCAAAACCGGCCACTGGAAAGCCTGTTCCTTCATCATCGGTATTTTTTTTTTTTTCAATTACTCTCTCTCATTTTTTTCCGTCCGAGACCGCCGTTGACCTCATAATTAATTTTTTCAGTGTATGAACTGATTGAAAGAATGATGTTCAGTGGGATTGCTGCAAATCTGATTATATATTTGACTATCAAACTCAATCAAGGCACTCTCACTGCCTCTAACAATGTCACCAATTGGACTGGAACCGTTTGGATTACGCCCATCCTTGGCGCTTACGTCGCTGACGCTTATCTCGGTCGCTATCGGACCTTCTTCATCTCCTCCCTCCTCTGCCTTGTGGTATGTCTCACTCTCAATTTCTCTTGTTTCCAAATTTTGGTTAGGTGAAATAGTTTAGAAAGAGTAATGATGAGGTGTAACTCCTTCCTTACTTCCTTACGAAAAAAATTAATTGCTAGATGTATTTTAGATTTTGGTGTGATATTTGGGTGTGTTTTTACTTTTTATTGTTAGGATGGGTTTGATTTATGGTCGAGTAGCAAAATGAATGAAAAAATATTTCGTTTTGAGTGATTTTTTTTTTAATTCTTATTATTGTTATTTTTCATGAGTTGTTTAGTTCTAATTTTCTTCTATGTTTGGACTGTTTATAGAGAAAAAATAGCTTAAGAACTTCTAAAGTAAAGTTGTTTATGTTATATAGATATTTAATTAGTATGTTTCCTTGTATATTTTTTCTATATAAAAAAATGAGTGAGTTTGTTGTAATGTAACACATCAAATTATGTCAATTATCGTTGAATTAAGCTCATTTTGATAAATAAAAAATTTGGTACATCCTAAAATGCATCAATATTCTTGTAACTTCCTTTTTTTGTGAGTACCTCGCAAATTCTTTTTTTAGTATAACAATTATGGGGATGAGGATCCTTCAACGTTTAGAGAGAAAGGTTATGTTTACCGTTATGCTAAGCTCACTTAAGCACCTTACAAATCTCTCTTAGTCACATACAAAAACACAAATTCAAAAGAGAAATTTTCACAGATAGAAAAATGTCAAATTATTTACAGAAAATAGCAAAAAAAAAAATACGGATAGACATTGATAGACTTCTGTCATTTCTATCACTGATAGACTTTAATAGACTTCTATCTGCATCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAGTTTACCTTATTTTTATATTTTTAAAAAAAATTCAATTCAAAAATATCTTTTTTTATTTAATAAAAAATAAATATGTCACGTGACAACTTGTAATTAAATGTCTAGAAAAAAATACAATCTAAAATGCACTAAGTATATGTATTTAAAAAGAAAAAAGAAAAAGAAAAAACTTTATTATTGGAGAAAAGTGAAGATGTAGAGGATGTGGAATTGAGATCCAAGTCCAACTAGGATAAAAATAAACAGGTGTTAACTGTTAAGGTAACAATGGGCACGTGCCCCCACCTCTTTAATTTTTATTTGTTTTCCAATGTTTTCCTTCATTTTTTAATTTATATATATATATATAACTTGTAGATCATATTTATTGACTTTGTAGTGATTGTTTTAACATATTTTTTTATGTAAAATATTAGATCGTCGTAGATAGATTTTAATAAAAACATTTTAAATTTCAAATTATGCTATTTTATATGTAACATATTAAACAAACATGTGCCTCCAATGCAATGGACCATATTAATAAACAAAAATGGACTCATCTTATTAATTTCATGTGAAAGTTGACTCTTGTTAAAATTGCACACAAGCCTTTTCTTATCAGATTTATATCATTATATTTATTAGATGATCTAATATGTAATTTATCTTCTCAAAATTTAAATTTTTTTGGTATATATATATATATAACTAAGATAGTATCAATAATTTTTAACTAGCTCAATGGTAATTGTATTTTTCAGTCTCCCTTAAACCCAAGTTGGAACATTAAGAAGGAAACGGTGTAAACTGACTATTCAATTTCATTTTAAAATTACTTTTAGATTTTTTTTTGTTTATTTTTTTTATAAGGCTTCAACTTCGATAATTCTTCACTTATTTAATTTTTTTCTTCAACTTCTTCTCTTAGTTTGTAAGGTAAGTTTTTTAGTTCATTTTTCTACTTCTTTTTAAGTTTGTTATTTTATTTGTGTACATTTAGTTTTCATGTTTTTATTACTTTTATACATTTATGTTATTTTTTGTTTTAGTTTTTTTATTTTATATATATATTATTATTTTTTAAAATTATTTATGAACCTTAAGTAAACATATTAAACTCTGTTAGTTGAGTTGTTTAAGTTATGATATTATTGTTATGTATTGATTTCAAAATATGAAATTTCATAAGAAACAATTATGATGTACTAAATTAAGTGAATTTATGAATTTCTAAAACTACTCACTTATAAATCAGTACAAGGTTAAGTTAAACATAGATTTTGACATAATTACAGGCAATGTCTCTTCTAACACTAGCAGTGTCAGTCCCAAGCCTAAAGCCCCCACCATGTTTAGAAGCTATTAACAAAGAAAATTGCAAACAAGCCTCCAAATTACAGCTTGCAGTGTTCTTTGGCTCACTCTACTTATTGGCGATTGCCTCCGGCGGGACGAAACCGAACATCTCGACAATGGGAGCCGACCAATTCGACGATTTTGATCCGAAGGAGAAGGCCCAAAAGCTGTCATTCTTCAACTGGTGGTTGTTTAGTGTATTCTCTGGCATTCTCTTTGCCTCTACTATTCTGGTTTACATTCAGGACAATGTTGGTTGGAGCTTAGGATATGGCATTCCCACTATTGGGCTGGGAGTTGCCATTCTTATATTTGTTGTTGGCACTCCCTTTTATAGACATAGGCTTCCTAATGGAAGCCCCTTCATTAGAATGGCTAATGTCATTGTTGCTGCTACTTGGAATTGGAGACTTCCTCTTCCTAATGACCCAAATCAACTATATGAGCTTGACCTTCAACATTACTCCAAGAATGGATCTTTCAAGATTGATTCCACTCCATCCTTGAGGTGGATGACATTCAATTAGTTTTGAGATTGAAGTGTAGGTTTATGCAACTTATGAAACAGATTATAATACATTGAAAATTGTATAATATGTAGGTTTCTGAATAAGGCTGCTATAAGAAGAGGTTCAAGTGATCCATGGAGGTTGTGCACAGTGACAGAAGTGGAGGAGACAAAACAAATGGTGAGAATGATACCAATTATGATATGCACATTCATACCAAGCACAATGGTGGCACAATCACACACCCTTTTCATCAAGCAAGGCACCACTTTGGATAGAAGCATTGGTAGCCACTTCAAAGTACCTCCTGCTAGTCTATATGCTTTTGTCACCATCTCCATGCTTCTCTCCATTCTCATCTACGACAGGTAATTATTCACTATTTTCTAAACTTGGGTAAAATTGCATGATTTTGAACACATGTTGTCCAAACCTTGTTGCTACCAAAATTCGAACAGGAGAAAATACATCTGACCTTCACATAACAGCAACAGTAAATTTATAATTTGGGAAATAATGTGACCTATACGATGGAAAGATTTGCGCACCAGTGTGGTACTTGCCACACAGGCTCTAATGCTTAAGTAAAGTGTATGTGCAGCCAGTAAAAAGTTTGAGTATAAGAATGAACTTACTTCTCTCTAGGCTGACCTAGGTTCTTTCTCATAGACTTTCGAAGTTGCATTGGGGTTATCTTTTGAATCATTAGGTTAGGGCATGCCCCGTTTGATATTTTGCTAGGGTCGGCAGAGCATGCGAGCTTGGGCCAAGTCCCAAGTCAACTCCTTTTTCTAGGCTCATAGTTCTAAGTTACCACTTTCAGCTTGAATCATGCTACTTTATTGACTTTGTTATTTCTATTTTTGCTTGATGTCGATAATGTCCTTTGGTTCAAAATCACTCCTTATGAACTAAAATAGATAAAAATAAGACATGATCCTTAGTTAATACATTTGTTTCCATTTCTTGGTACAGAATATTTGTGAAGATAATGCAAAAAGTGACAAAAAATCCAAGGGGAATCACAATGTTACAAAGAATGGGAATTGGAATGATTTGTCATATTTTGGTAATGACAGTTGCTTCTCAAGTGGAAAAGCATAGACTTAATATTGCTGCAGAAAATGGATCATCATTATCACAAGAACAAAAAGTACTTCCCTTAACCATTTTCATCCTCCTCCCTCAGTTCATCCTCACAGGAGTTGCTGATGCATTCCTTCAAATAGCCAGTAATGAATTCTTTTATGATCAAGCACCAGAAAACATGAAGAGTTTAGGCAGTTCATATTTTATGACTTCACTTGGAATTGGGAACTTCCTCAGTACTTTTATTCTTTCAAAAGTTTCTGAGATTACCAAAAGACAAGGCAAAGGCTGGATTTTGAACAACTTGAATGTTTCTCATCTTGATTACTTCTATGCTTTACTTGCAGTTATGAGTGCTGTAAACTTCTTCCTCTTTCTGCTCATTTCCAAATTATATGTCTACAAAGCTGAAGTCTCTGATTCCATTAAACTGCTTACTGATGAACTCAAGAAGAAGAAATCAAAGGGCCTCCAACAAACAGGTTGA

mRNA sequence

GATATATGCCACATGGCAAAGTTTTTGATTGATATAGAGTATCAGCTGCATCCAAGTGTTTTTACAAAAGGGCAGTGGTGTCTCATATTTCATAAACAAATACCCTTTGTGTTGGAGCTGCTTCATAGAAGTAATAAAAAGAGAGAGAAATAGGGAAAAGGAAAATCATCATCATCATCATAATAATAATAGTACTTCCTCCGCCATTAATGGCGGGTGCTGCAGCAGATCAAGAAACCGGGCTCGACGATTACACCAAAGATGGAACCGTGGATCGGAAAGGCAACCCGGTTCTCCGCTCCAAAACCGGCCACTGGAAAGCCTGTTCCTTCATCATCGTGTATGAACTGATTGAAAGAATGATGTTCAGTGGGATTGCTGCAAATCTGATTATATATTTGACTATCAAACTCAATCAAGGCACTCTCACTGCCTCTAACAATGTCACCAATTGGACTGGAACCGTTTGGATTACGCCCATCCTTGGCGCTTACGTCGCTGACGCTTATCTCGGTCGCTATCGGACCTTCTTCATCTCCTCCCTCCTCTGCCTTGTGCCCCCACCATGTTTAGAAGCTATTAACAAAGAAAATTGCAAACAAGCCTCCAAATTACAGCTTGCAGTGTTCTTTGGCTCACTCTACTTATTGGCGATTGCCTCCGGCGGGACGAAACCGAACATCTCGACAATGGGAGCCGACCAATTCGACGATTTTGATCCGAAGGAGAAGGCCCAAAAGCTGTCATTCTTCAACTGGTGGTTGTTTAGTGTATTCTCTGGCATTCTCTTTGCCTCTACTATTCTGGTTTACATTCAGGACAATGTTGGTTGGAGCTTAGGATATGGCATTCCCACTATTGGGCTGGGAGTTGCCATTCTTATATTTGTTGTTGGCACTCCCTTTTATAGACATAGGCTTCCTAATGGAAGCCCCTTCATTAGAATGGCTAATGTCATTGTTGCTGCTACTTGGAATTGGAGACTTCCTCTTCCTAATGACCCAAATCAACTATATGAGCTTGACCTTCAACATTACTCCAAGAATGGATCTTTCAAGATTGATTCCACTCCATCCTTGAGGTTTCTGAATAAGGCTGCTATAAGAAGAGGTTCAAGTGATCCATGGAGGTTGTGCACAGTGACAGAAGTGGAGGAGACAAAACAAATGGTGAGAATGATACCAATTATGATATGCACATTCATACCAAGCACAATGGTGGCACAATCACACACCCTTTTCATCAAGCAAGGCACCACTTTGGATAGAAGCATTGGTAGCCACTTCAAAGTACCTCCTGCTAGTCTATATGCTTTTGTCACCATCTCCATGCTTCTCTCCATTCTCATCTACGACAGAATATTTGTGAAGATAATGCAAAAAGTGACAAAAAATCCAAGGGGAATCACAATGTTACAAAGAATGGGAATTGGAATGATTTGTCATATTTTGGTAATGACAGTTGCTTCTCAAGTGGAAAAGCATAGACTTAATATTGCTGCAGAAAATGGATCATCATTATCACAAGAACAAAAAGTACTTCCCTTAACCATTTTCATCCTCCTCCCTCAGTTCATCCTCACAGGAGTTGCTGATGCATTCCTTCAAATAGCCAGTAATGAATTCTTTTATGATCAAGCACCAGAAAACATGAAGAGTTTAGGCAGTTCATATTTTATGACTTCACTTGGAATTGGGAACTTCCTCAGTACTTTTATTCTTTCAAAAGTTTCTGAGATTACCAAAAGACAAGGCAAAGGCTGGATTTTGAACAACTTGAATGTTTCTCATCTTGATTACTTCTATGCTTTACTTGCAGTTATGAGTGCTGTAAACTTCTTCCTCTTTCTGCTCATTTCCAAATTATATGTCTACAAAGCTGAAGTCTCTGATTCCATTAAACTGCTTACTGATGAACTCAAGAAGAAGAAATCAAAGGGCCTCCAACAAACAGGTTGA

Coding sequence (CDS)

ATGGCGGGTGCTGCAGCAGATCAAGAAACCGGGCTCGACGATTACACCAAAGATGGAACCGTGGATCGGAAAGGCAACCCGGTTCTCCGCTCCAAAACCGGCCACTGGAAAGCCTGTTCCTTCATCATCGTGTATGAACTGATTGAAAGAATGATGTTCAGTGGGATTGCTGCAAATCTGATTATATATTTGACTATCAAACTCAATCAAGGCACTCTCACTGCCTCTAACAATGTCACCAATTGGACTGGAACCGTTTGGATTACGCCCATCCTTGGCGCTTACGTCGCTGACGCTTATCTCGGTCGCTATCGGACCTTCTTCATCTCCTCCCTCCTCTGCCTTGTGCCCCCACCATGTTTAGAAGCTATTAACAAAGAAAATTGCAAACAAGCCTCCAAATTACAGCTTGCAGTGTTCTTTGGCTCACTCTACTTATTGGCGATTGCCTCCGGCGGGACGAAACCGAACATCTCGACAATGGGAGCCGACCAATTCGACGATTTTGATCCGAAGGAGAAGGCCCAAAAGCTGTCATTCTTCAACTGGTGGTTGTTTAGTGTATTCTCTGGCATTCTCTTTGCCTCTACTATTCTGGTTTACATTCAGGACAATGTTGGTTGGAGCTTAGGATATGGCATTCCCACTATTGGGCTGGGAGTTGCCATTCTTATATTTGTTGTTGGCACTCCCTTTTATAGACATAGGCTTCCTAATGGAAGCCCCTTCATTAGAATGGCTAATGTCATTGTTGCTGCTACTTGGAATTGGAGACTTCCTCTTCCTAATGACCCAAATCAACTATATGAGCTTGACCTTCAACATTACTCCAAGAATGGATCTTTCAAGATTGATTCCACTCCATCCTTGAGGTTTCTGAATAAGGCTGCTATAAGAAGAGGTTCAAGTGATCCATGGAGGTTGTGCACAGTGACAGAAGTGGAGGAGACAAAACAAATGGTGAGAATGATACCAATTATGATATGCACATTCATACCAAGCACAATGGTGGCACAATCACACACCCTTTTCATCAAGCAAGGCACCACTTTGGATAGAAGCATTGGTAGCCACTTCAAAGTACCTCCTGCTAGTCTATATGCTTTTGTCACCATCTCCATGCTTCTCTCCATTCTCATCTACGACAGAATATTTGTGAAGATAATGCAAAAAGTGACAAAAAATCCAAGGGGAATCACAATGTTACAAAGAATGGGAATTGGAATGATTTGTCATATTTTGGTAATGACAGTTGCTTCTCAAGTGGAAAAGCATAGACTTAATATTGCTGCAGAAAATGGATCATCATTATCACAAGAACAAAAAGTACTTCCCTTAACCATTTTCATCCTCCTCCCTCAGTTCATCCTCACAGGAGTTGCTGATGCATTCCTTCAAATAGCCAGTAATGAATTCTTTTATGATCAAGCACCAGAAAACATGAAGAGTTTAGGCAGTTCATATTTTATGACTTCACTTGGAATTGGGAACTTCCTCAGTACTTTTATTCTTTCAAAAGTTTCTGAGATTACCAAAAGACAAGGCAAAGGCTGGATTTTGAACAACTTGAATGTTTCTCATCTTGATTACTTCTATGCTTTACTTGCAGTTATGAGTGCTGTAAACTTCTTCCTCTTTCTGCTCATTTCCAAATTATATGTCTACAAAGCTGAAGTCTCTGATTCCATTAAACTGCTTACTGATGAACTCAAGAAGAAGAAATCAAAGGGCCTCCAACAAACAGGTTGA

Protein sequence

MAGAAADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTG
Homology
BLAST of Lsi05G017580 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.6e-207
Identity = 355/579 (61.31%), Postives = 451/579 (77.89%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GNPV RS  G WKACSF++VYE+ ERM + GI++NL IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCL------------ 127
           L+QGT+ +SNNVTNW GT W+TPILGAYV DA LGRY TF IS  +              
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTI 123

Query: 128 --VPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKE 187
             + PP     N ENC++AS LQLAVFFG+LY LAI +GGTKPNIST+GADQFD FDPKE
Sbjct: 124 PGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPKE 183

Query: 188 KAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFY 247
           K QKLSFFNWW+FS+F G LFA+T+LVY+QDNVGW+LGYG+PT+GL ++I IF++GTPFY
Sbjct: 184 KTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPFY 243

Query: 248 RHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFL 307
           RH+LP GSPF +MA VIVA+      P+ +D    +EL    Y + G+F I  TPSLRFL
Sbjct: 244 RHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRFL 303

Query: 308 NKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDR 367
           ++A+++ G++  W LCT TEVEETKQM+RM+P++  TF+PS M+AQ +TLF+KQGTTLDR
Sbjct: 304 DRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLDR 363

Query: 368 SIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHI 427
            +   F +PPASL  FVT+SML+SI++YDR+FVKI +K T NPRGIT+LQRMGIG+I HI
Sbjct: 364 KVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFHI 423

Query: 428 LVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFF 487
           L+M VAS  E++RL +AA++G  + Q    LPLTIF LLPQF+L G+AD+FL++A  EFF
Sbjct: 424 LIMIVASVTERYRLKVAADHG-LIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEFF 483

Query: 488 YDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFYA 547
           YDQAPE+MKSLG+SY  TSL IGNF+S+F+LS VSEITK++G+GWILNNLN S LDY+Y 
Sbjct: 484 YDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESRLDYYYL 543

Query: 548 LLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKK 573
             AV++ VNF LFL++ K YVY+AEV+DS+ +   E+K+
Sbjct: 544 FFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Lsi05G017580 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 4.6e-199
Identity = 340/570 (59.65%), Postives = 443/570 (77.72%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GN V RS+TG WKACSF++VYE+ ERM + GI++NL+IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLV----------- 127
           L+QGT+ +SNNVTNW GT W+TPILGAYVADA+ GRY TF ISS + L+           
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSL 123

Query: 128 ----PPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
               PP C  A N ENC++AS +QLAVFFG+LY LAI +GGTKPNIST+GADQFD+FDPK
Sbjct: 124 PGLKPPKCSTA-NVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 183

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           +K  K SFFNWW+FS+F G  FA+T+LVY+QDNVGW++GYG+ T+GL  +I IF++GT  
Sbjct: 184 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 243

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRH+LP GSPF +MA VIVA+    R P+ +D  + YEL    Y+   +F I ST SLRF
Sbjct: 244 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRF 303

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           LN+A+++ GS+  WRLCT+TEVEETKQM++M+P++  TF+PS M+AQ  TLFIKQGTTLD
Sbjct: 304 LNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLD 363

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           R + ++F +PPASL  F T SML+SI+IYDR+FVK M+K+T NPRGIT+LQRMGIGMI H
Sbjct: 364 RRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILH 423

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           IL+M +AS  E++RL +AAE+G +  Q    +PL+IF LLPQ++L G+ADAF++IA  EF
Sbjct: 424 ILIMIIASITERYRLKVAAEHGLT-HQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEF 483

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPE+MKSLG+SY  TS+ +G F+S+ +LS VS+ITK+QG+GWI NNLN S LD +Y
Sbjct: 484 FYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRLDNYY 543

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDS 563
              AV++ +NF LFL++ + Y Y+A+V+ S
Sbjct: 544 MFFAVLNLLNFILFLVVIRFYEYRADVTQS 570

BLAST of Lsi05G017580 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 551.2 bits (1419), Expect = 1.4e-155
Identity = 284/560 (50.71%), Postives = 392/560 (70.00%), Query Frame = 0

Query: 15  YTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLT 74
           YT+DGTVD +G PVL SKTG W+ACSF++ YE  ERM F GIA+NL+ YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCL--------------VPPPC 134
           +  NV NW+G VWITPI GAY+AD+Y+GR+ TF  SSL+ +              + P C
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRPTC 126

Query: 135 LEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLSF 194
              +    C +AS LQ+  F+ SLY +AI +GGTKPNIST GADQFD +  +EK QK+SF
Sbjct: 127 ENGV----CNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 195 FNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPNG 254
           FNWW+FS F G LFA+  LVYIQ+N+GW LGYGIPT+GL V++++F +GTPFYRH++   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 255 SPFIR-MANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAIR 314
               + +  V +AA  N +L  P+D  +LYELD  +Y  NG  ++  TP  RFL+KAAI+
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAIK 306

Query: 315 RGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSIGSHF 374
             S  P   CTVT+VE  K+++ +I I + T IPST+ AQ +TLF+KQGTTLDR IGS+F
Sbjct: 307 TSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGSNF 366

Query: 375 KVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILVMTVA 434
           ++P ASL +FVT+SMLLS+ +YD+ FV  M+K T NPRGIT+LQR+G+G    I+ + +A
Sbjct: 367 QIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIAIA 426

Query: 435 SQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYDQAPE 494
           S VE  R+ +  E    ++   +V+P++IF LLPQ+ L G+ D F  I   EFFYDQ+PE
Sbjct: 427 SAVEVKRMRVIKE--FHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSPE 486

Query: 495 NMKSLGSSYFMTSLGIGNFLSTFILSKVSEIT-KRQGKGWILNNLNVSHLDYFYALLAVM 554
            M+SLG+++F + +G+GNFL++F+++ + +IT K  GK WI NNLN S LDY+Y  L V+
Sbjct: 487 EMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLVVI 546

Query: 555 SAVNFFLFLLISKLYVYKAE 559
           S VN  LF+  +  YVYK++
Sbjct: 547 SIVNMGLFVWAASKYVYKSD 557

BLAST of Lsi05G017580 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 6.3e-132
Identity = 247/565 (43.72%), Postives = 363/565 (64.25%), Query Frame = 0

Query: 13  DDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGT 72
           D YT+DGTVD   NP  + KTG+WKAC FI+  E  ER+ + G+  NL+ YL  +LNQG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 73  LTASNNVTNWTGTVWITPILGAYVADAYLGRYRT------FFISSLLCLVPPPCLEAINK 132
            TA+NNVTNW+GT +ITP++GA++ADAYLGRY T       ++S +  L     +  +  
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 133 ENCK----QASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLSFFN 192
            NC       +  Q AVFF +LY++A+ +GG KP +S+ GADQFD+ D  EK +K SFFN
Sbjct: 125 GNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFN 184

Query: 193 WWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPNGSP 252
           W+ FS+  G L A+T+LV+IQ NVGW  G+G+PT+ + +A+  F  G+ FYR + P GSP
Sbjct: 185 WFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSP 244

Query: 253 FIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAA----- 312
             R+  VIVAA     + +P D + L+E      +  GS K+  T +L+F +KAA     
Sbjct: 245 LTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQS 304

Query: 313 --IRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSI 372
             I+ G  +PWRLC+VT+VEE K ++ ++P+     + +T+ +Q  T+F+ QG T+D+ +
Sbjct: 305 DSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHM 364

Query: 373 GSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILV 432
           G +F++P ASL  F T+S+L    +YD+  + + +K T+N RG T LQRMGIG++  I  
Sbjct: 365 GKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFA 424

Query: 433 MTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYD 492
           M  A  +E  RL+    +    + +QK + ++IF  +PQ++L G A+ F  I   EFFYD
Sbjct: 425 MITAGVLEVVRLDYVKTHN---AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYD 484

Query: 493 QAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGK-GWILNNLNVSHLDYFYAL 552
           QAP+ M+SL S+  +T++ +GN+LST +++ V +ITK+ GK GWI +NLN  HLDYF+ L
Sbjct: 485 QAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYL 544

Query: 553 LAVMSAVNFFLFLLISKLYVYKAEV 560
           LA +S +NF ++L ISK Y YK  V
Sbjct: 545 LATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Lsi05G017580 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 3.7e-124
Identity = 242/566 (42.76%), Postives = 354/566 (62.54%), Query Frame = 0

Query: 9   ETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKL 68
           E   D YTKDGT+D    P  ++KTG WKAC FI+  E  ER+ + G++ NLI YL  ++
Sbjct: 2   EDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQM 61

Query: 69  NQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRT------FFISSLLCLV----PP 128
           N   ++AS +V+NW+GT + TP++GA++ADAYLGRY T       +I+ +  L      P
Sbjct: 62  NMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVP 121

Query: 129 PCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKL 188
                 + E C  A+  Q A+ F +LYL+A+ +GG KP +S+ GADQFDD D KEK  K 
Sbjct: 122 GLTPTCSGETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKS 181

Query: 189 SFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLP 248
           SFFNW+ F +  G + AS++LV+IQ NVGW  G G+PT+ + +A++ F  G+ FYR + P
Sbjct: 182 SFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKP 241

Query: 249 NGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAI 308
            GSP  RM  VIVA+    ++ +P D + LYE      S  GS K++ T  L F +KAA+
Sbjct: 242 GGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAV 301

Query: 309 RRGS-------SDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTL 368
              S       S  W+LCTVT+VEE K ++R++PI     + +++ +Q  T+F+ QG TL
Sbjct: 302 ETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTL 361

Query: 369 DRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMIC 428
           D+ +G +FK+P ASL  F T+S+L    +YD++ V   +K T + RG T LQR+GIG++ 
Sbjct: 362 DQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVI 421

Query: 429 HILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNE 488
            I  M  A  +E  RLN    +       ++ +P+TIF  +PQ+ L G A+ F  I   E
Sbjct: 422 SIFSMVSAGILEVARLNYVQTHN---LYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLE 481

Query: 489 FFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGK-GWILNNLNVSHLDY 548
           FFYDQAP+ M+SL S+  +T++  GN+LSTF+++ V+++T+  G+ GWI  NLN  HLDY
Sbjct: 482 FFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDY 541

Query: 549 FYALLAVMSAVNFFLFLLISKLYVYK 557
           F+ LLA +S +NF ++L I+K Y YK
Sbjct: 542 FFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of Lsi05G017580 vs. ExPASy TrEMBL
Match: A0A5A7U6G8 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G004610 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.3e-289
Identity = 514/593 (86.68%), Postives = 548/593 (92.41%), Query Frame = 0

Query: 1   MAGAAADQETGLD--DYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAA 60
           MA AAA+QE GLD  +YTKDGTVD KGN VLRSKTG WKACSFI+ YELIERMMF GI++
Sbjct: 1   MADAAANQEAGLDLHNYTKDGTVDWKGNTVLRSKTGRWKACSFILGYELIERMMFHGISS 60

Query: 61  NLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLC---- 120
           NLIIYLT KLNQGTLTASNNVTNW+GTVW  PI+GAYVADA+LGRYRTFFISSL+C    
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWSGTVWTMPIIGAYVADAHLGRYRTFFISSLVCFMAM 120

Query: 121 -----------LVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180
                      L PPPC  +I++ENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA
Sbjct: 121 TLLTAAVSIPSLKPPPCSASISRENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240
           DQFDDFDPKEKAQKLSFFNWWLF+VFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240

Query: 241 LIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFK 300
           LIFVVGTPFYRH+ PNGSPFI MANV VAA WNWRLPLPNDPNQL+ELDLQ+YSKNG+FK
Sbjct: 241 LIFVVGTPFYRHKPPNGSPFITMANVFVAAIWNWRLPLPNDPNQLHELDLQNYSKNGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTL 360
           IDSTPSLRFLNKAAIRR SSDPWR+CTVTEVEETKQMVRMIPIM+C+FIPS MVAQ+HTL
Sbjct: 301 IDSTPSLRFLNKAAIRRVSSDPWRICTVTEVEETKQMVRMIPIMVCSFIPSAMVAQTHTL 360

Query: 361 FIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQ 420
           FIKQGTTL+RSIGSHFKVPPASLYAFVTISMLL+ILIYDRIF+KIMQ+VTKNPRGITMLQ
Sbjct: 361 FIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFLKIMQRVTKNPRGITMLQ 420

Query: 421 RMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADA 480
           RMGIGMICH+LVMTVASQVEKHRL+IAA+ GSS + EQK LPLTIFILLPQFILTGVADA
Sbjct: 421 RMGIGMICHVLVMTVASQVEKHRLHIAAKYGSS-AHEQKELPLTIFILLPQFILTGVADA 480

Query: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNL 540
           FL IA+NEFFYDQAPENMKSLGSSYF TSLGIGNFLSTFILSKVSEITKRQG GWILNNL
Sbjct: 481 FLLIANNEFFYDQAPENMKSLGSSYFTTSLGIGNFLSTFILSKVSEITKRQGNGWILNNL 540

Query: 541 NVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSK 577
           N SHL+YFYALLAVMS+VNFFLFLLISK YVYKAEVSDSI++LTDELKKKKSK
Sbjct: 541 NSSHLNYFYALLAVMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSK 592

BLAST of Lsi05G017580 vs. ExPASy TrEMBL
Match: A0A0A0L9Q0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134750 PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 3.6e-271
Identity = 469/583 (80.45%), Postives = 517/583 (88.68%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           QE+ +D+YT+DGTVD KG P+LRSKTG WKAC FIIVYEL+ER++F G++ANL IYLT K
Sbjct: 9   QESVIDNYTQDGTVDLKGRPLLRSKTGSWKACFFIIVYELLERIVFQGVSANLFIYLTTK 68

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLL-------------- 127
           L+QG +TASNNVTNW G +WI PI GAY+ADA+LGRYRTF ISS +              
Sbjct: 69  LHQGIVTASNNVTNWNGAIWIMPIFGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSV 128

Query: 128 -CLVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
             L PPPCL+ I K+NCKQASKLQLAVFFGSLYLL IASGGTKPNISTMGADQFDDF PK
Sbjct: 129 PSLKPPPCLQPITKQNCKQASKLQLAVFFGSLYLLVIASGGTKPNISTMGADQFDDFYPK 188

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           EKAQKLSFFNWW F+ FSGILFASTILVYIQDNVGWSLGYGIPTIG+GVAI+IFVVGTPF
Sbjct: 189 EKAQKLSFFNWWFFTAFSGILFASTILVYIQDNVGWSLGYGIPTIGIGVAIIIFVVGTPF 248

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRHR P+GSPF  +ANVIV A WNWRLP+PNDPNQLYEL++QHYSK G+FKIDSTPSLRF
Sbjct: 249 YRHRPPSGSPFTTIANVIVGAAWNWRLPIPNDPNQLYELEIQHYSKPGNFKIDSTPSLRF 308

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           LNKAAIRR S  PWRLC+VTEVEETKQM+RMIPI+ICTFIP T+ AQ+HTLFIKQGTTLD
Sbjct: 309 LNKAAIRRDSGGPWRLCSVTEVEETKQMLRMIPILICTFIPHTITAQTHTLFIKQGTTLD 368

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           RSIGSHFKVPPASLYAFVTIS+LLSILIYDRIFVKI+++VTKNPRGITMLQRMGIGMICH
Sbjct: 369 RSIGSHFKVPPASLYAFVTISLLLSILIYDRIFVKIVRRVTKNPRGITMLQRMGIGMICH 428

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           +LVM VAS+VEKHRL++AA    S  QEQKVLPLTIF LLPQFILTGVAD+FLQ+A  EF
Sbjct: 429 VLVMVVASRVEKHRLDVAARENGSSPQEQKVLPLTIFTLLPQFILTGVADSFLQVAVQEF 488

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPENMKS G+SY MTSLGIGNFLS+ I+SKVSEITKRQGK WILNNLN SHLDYFY
Sbjct: 489 FYDQAPENMKSFGTSYAMTSLGIGNFLSSLIVSKVSEITKRQGKEWILNNLNASHLDYFY 548

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKS 576
            LLAVMSAVNFFLFL+ISKLY+YKAEVSDSI LLT+ELKKKKS
Sbjct: 549 LLLAVMSAVNFFLFLIISKLYLYKAEVSDSINLLTEELKKKKS 591

BLAST of Lsi05G017580 vs. ExPASy TrEMBL
Match: A0A5D3D0T3 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001900 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 8.8e-270
Identity = 492/599 (82.14%), Postives = 519/599 (86.64%), Query Frame = 0

Query: 1   MAGAAADQETGLD--DYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAA 60
           MA AAADQE GLD   YTKDGTVD K NPVLRSKTG WKACSFI+ YELIERMMF+GI+A
Sbjct: 1   MADAAADQEAGLDLQSYTKDGTVDWKANPVLRSKTGRWKACSFILGYELIERMMFNGISA 60

Query: 61  NLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLC---- 120
           NLIIYLT KLNQGTLTASNNVTNWTGTVWITPILGAYVADA+LGRYRTFFISSL+C    
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWTGTVWITPILGAYVADAHLGRYRTFFISSLVCFMAM 120

Query: 121 -----------LVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180
                      L PPPC  +I++ENCKQASKLQLA+FFGSLYLLAIASGGTKPNISTMGA
Sbjct: 121 ALLTAAVSIPRLKPPPCSASISRENCKQASKLQLALFFGSLYLLAIASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240
           DQFDDFDPKEKAQKLSFFNWWLF+VFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240

Query: 241 LIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFK 300
           LIFVVGTPFYR +LPNGSPFI MA+VIVAA WNWRLPLPNDPNQL+ELDLQHYSKNG+FK
Sbjct: 241 LIFVVGTPFYRLKLPNGSPFITMADVIVAAIWNWRLPLPNDPNQLHELDLQHYSKNGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTL 360
           IDSTPSLRFLNKAAIRR   DP R CTVTEVEETKQMVRMIPIM+CTFIPS MVAQ+HTL
Sbjct: 301 IDSTPSLRFLNKAAIRRVLRDPRRTCTVTEVEETKQMVRMIPIMVCTFIPSAMVAQTHTL 360

Query: 361 FIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQ 420
           FIKQGTTL+RSIGSHFKVPPASLYAFVTISMLL+ILIYDRIFVKIMQ+VTKNPRGITMLQ
Sbjct: 361 FIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFVKIMQRVTKNPRGITMLQ 420

Query: 421 RMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADA 480
           RMGIGMICH+LVMTVASQVEKHRL+IAA+ GSS + EQK LPLTIFILLPQFILTGVADA
Sbjct: 421 RMGIGMICHVLVMTVASQVEKHRLHIAAKYGSS-AHEQKELPLTIFILLPQFILTGVADA 480

Query: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNL 540
           FLQIASNEFFYDQAPENMKSLGSSYFMTS GIGNFL                        
Sbjct: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSFGIGNFL------------------------ 540

Query: 541 NVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTG 583
                        +MS+VNFFLFLLISK YVYKAEVSDSI++LTDELKKKKSKGLQQTG
Sbjct: 541 -------------IMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSKGLQQTG 561

BLAST of Lsi05G017580 vs. ExPASy TrEMBL
Match: A0A6J1E9F4 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431936 PE=3 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 1.9e-264
Identity = 476/592 (80.41%), Postives = 516/592 (87.16%), Query Frame = 0

Query: 2   AGAAADQETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLI 61
           A AA DQE+GLDDYTKDGTVDRKGNP LRS TG WKACSFI+VYELI+RMMF+GIAANLI
Sbjct: 3   AAAAEDQESGLDDYTKDGTVDRKGNPTLRSNTGGWKACSFIVVYELIDRMMFNGIAANLI 62

Query: 62  IYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLL-------- 121
           IYLT KLNQGT+TASNNVTNWTGTVWITPI GAYVADA+LG YRTFFISSL         
Sbjct: 63  IYLTTKLNQGTVTASNNVTNWTGTVWITPIFGAYVADAHLGCYRTFFISSLASFMAMSLL 122

Query: 122 -------CLVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQF 181
                   L PPPCLE  +KENCKQASKLQLAVFFGSLY+LA+ASGGTKPNISTMGADQF
Sbjct: 123 TVAVSVPSLQPPPCLEP-SKENCKQASKLQLAVFFGSLYMLAVASGGTKPNISTMGADQF 182

Query: 182 DDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIF 241
           DDF PKEK+QKLSFFNWW+FSVFSGILFASTILVYIQDNVGWS GYGIPTIGLGVAILIF
Sbjct: 183 DDFHPKEKSQKLSFFNWWMFSVFSGILFASTILVYIQDNVGWSFGYGIPTIGLGVAILIF 242

Query: 242 VVGTPFYRHRLPN-GSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKID 301
           V GTPFYRHRLP+ GSPF+RMA VIVAA  NWR+PLPNDPNQLYEL++Q Y      KID
Sbjct: 243 VAGTPFYRHRLPSGGSPFLRMARVIVAAARNWRVPLPNDPNQLYELEVQQYP-----KID 302

Query: 302 STPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFI 361
           STPS RFLNKAA+R GSS PWR CTVT+VEETKQM+RMIPI+ICTFIPSTMVAQSHTLFI
Sbjct: 303 STPSFRFLNKAAVRTGSSHPWRSCTVTQVEETKQMLRMIPILICTFIPSTMVAQSHTLFI 362

Query: 362 KQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRM 421
           KQGTTLDR+IGSHFKVPPASLYAFVTISMLLSI+IYDRIFVKIMQ+VT+NPRGITMLQRM
Sbjct: 363 KQGTTLDRTIGSHFKVPPASLYAFVTISMLLSIVIYDRIFVKIMQRVTRNPRGITMLQRM 422

Query: 422 GIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFL 481
           GIGMI H+LVMTVAS+VEK RL++A  NG   +   +VLPLTIF LLPQF+LTGVADA L
Sbjct: 423 GIGMIFHVLVMTVASRVEKRRLHVARANGLVRNGSGQVLPLTIFTLLPQFMLTGVADALL 482

Query: 482 QIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNV 541
           QIA+ EFFYDQAP++MKSLGSSY MTSLGIGNFLS+F+LSKVSEITKR G+GWILNNLN 
Sbjct: 483 QIANVEFFYDQAPKSMKSLGSSYMMTSLGIGNFLSSFVLSKVSEITKRHGEGWILNNLNA 542

Query: 542 SHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKG 578
           SHLDYFYALLA MS VNFF+FL IS+LYVY+AEVS       DEL KK   G
Sbjct: 543 SHLDYFYALLAAMSGVNFFVFLGISQLYVYRAEVS-------DELNKKNEVG 581

BLAST of Lsi05G017580 vs. ExPASy TrEMBL
Match: A0A6J1CQA1 (protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111013596 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 5.2e-262
Identity = 457/597 (76.55%), Postives = 524/597 (87.77%), Query Frame = 0

Query: 1   MAGAAADQETGL--DDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAA 60
           M G A + E G+  DDYT+DG+VD KGNPVLR KTG WKACSFIIVYE+I+RMMF+GI+A
Sbjct: 1   MGGVAEEVEGGVVNDDYTQDGSVDLKGNPVLRRKTGRWKACSFIIVYEVIDRMMFNGISA 60

Query: 61  NLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLC---- 120
           NLIIYLT KLNQGT+TASNNVTNW+GT+WITP+LGAY+ADA+LGRYRTF IS+LLC    
Sbjct: 61  NLIIYLTTKLNQGTVTASNNVTNWSGTLWITPVLGAYIADAHLGRYRTFLISTLLCFTSM 120

Query: 121 -----------LVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180
                      L PPPCLEA NKENCKQASKLQL VFFG+LY LA+ASGGTKPNISTMGA
Sbjct: 121 AVLTVAVSVPSLKPPPCLEA-NKENCKQASKLQLGVFFGALYALAVASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240
           DQFD+FDPKEKAQKLSFFNWWLFSVF GILFAST+LVYIQDNVGW+LGYGIPT GL VAI
Sbjct: 181 DQFDEFDPKEKAQKLSFFNWWLFSVFFGILFASTVLVYIQDNVGWALGYGIPTSGLAVAI 240

Query: 241 LIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFK 300
           LIF+ GTPFYRH+LP+GSPF RMA VIVAA +NW LPLP DP QL+ELDL+HYSK G+F+
Sbjct: 241 LIFLAGTPFYRHKLPSGSPFSRMAKVIVAAAFNWTLPLPKDPKQLHELDLEHYSKPGTFR 300

Query: 301 IDSTPSLRFLNKAAIRRGSSD----PWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQ 360
           IDSTPSLR LNKAAI+  SS     PW+LC+VT+VEETKQM+RMIPI+ICTFIPSTM+AQ
Sbjct: 301 IDSTPSLRILNKAAIKTVSSSTTDHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLAQ 360

Query: 361 SHTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGI 420
           +HTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISML++I++YDR+FVKIMQ++TKNPRGI
Sbjct: 361 AHTLFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLVTIVVYDRVFVKIMQRLTKNPRGI 420

Query: 421 TMLQRMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTG 480
           T+LQRMGIGMI H+L+MTVAS+VE+HRL++A ENG   +    VLPL+IF LLPQF+L G
Sbjct: 421 TLLQRMGIGMILHVLIMTVASRVERHRLDVARENGLVKNGGHAVLPLSIFTLLPQFMLMG 480

Query: 481 VADAFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWI 540
           +ADAF+QIAS EFFYDQAPE MKSLGSSY MTSLGIGNFLS+F+LS VS+IT ++G GWI
Sbjct: 481 IADAFVQIASVEFFYDQAPETMKSLGSSYMMTSLGIGNFLSSFLLSTVSDITNKRGNGWI 540

Query: 541 LNNLNVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSK 577
            NNLN SHLDYFYALLAV++ VNF +FL++SK+YVYKAEVSDSIKLLT+ LKKKK K
Sbjct: 541 QNNLNASHLDYFYALLAVLNVVNFCIFLVVSKMYVYKAEVSDSIKLLTEGLKKKKLK 596

BLAST of Lsi05G017580 vs. NCBI nr
Match: XP_038874856.1 (protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.5e-308
Identity = 540/599 (90.15%), Postives = 567/599 (94.66%), Query Frame = 0

Query: 1   MAGAAADQETG--LDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAA 60
           MAGAAADQETG  LD+YTKDGTVDRKGNPVLRSKTG WKACSFIIVYELI+RMMF+GIAA
Sbjct: 1   MAGAAADQETGLQLDNYTKDGTVDRKGNPVLRSKTGRWKACSFIIVYELIDRMMFNGIAA 60

Query: 61  NLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLV-- 120
           NLIIYLT KLNQGTLTASNNVTNWTGTVWITPILGAYVADA+LGRYRTFFISSLLCL+  
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWTGTVWITPILGAYVADAHLGRYRTFFISSLLCLIAM 120

Query: 121 -------------PPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180
                        PPPCLEAI+KENCKQASKLQLAVFFGSLYLLA+ASGGTKPNISTMGA
Sbjct: 121 SLLTLAVSVPSLKPPPCLEAISKENCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240
           DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIP IGLGVAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPPIGLGVAI 240

Query: 241 LIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFK 300
           LIFVVGTPFYRHRLPNGSPF  MANVI+AA WNWR PLPNDPN+LYEL++QHYSK+G+FK
Sbjct: 241 LIFVVGTPFYRHRLPNGSPFTTMANVIIAAIWNWRFPLPNDPNELYELEIQHYSKHGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTL 360
           IDSTPSLRFLNKAAIRR SSDPWR+CTVTEVEETKQMVRMIPIMICTFIP+TMVAQSHTL
Sbjct: 301 IDSTPSLRFLNKAAIRRDSSDPWRMCTVTEVEETKQMVRMIPIMICTFIPNTMVAQSHTL 360

Query: 361 FIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQ 420
           FIKQGTTLDRSIGSHFKVPPASLY+FVTISMLLSILIYDR+FVKIMQ+VTKNPRGITMLQ
Sbjct: 361 FIKQGTTLDRSIGSHFKVPPASLYSFVTISMLLSILIYDRVFVKIMQRVTKNPRGITMLQ 420

Query: 421 RMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADA 480
           RMGIGMICH+L+MTVASQVEKHRL+IA ENG S +QEQKVLPLTIFILLPQFILTG+ADA
Sbjct: 421 RMGIGMICHVLIMTVASQVEKHRLHIAEENGLSAAQEQKVLPLTIFILLPQFILTGIADA 480

Query: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNL 540
           FLQIASNEFFYDQAPENMKSLG+SYFMTS GIGNFLSTFILSKVS+ITKRQGKGWILNNL
Sbjct: 481 FLQIASNEFFYDQAPENMKSLGNSYFMTSHGIGNFLSTFILSKVSKITKRQGKGWILNNL 540

Query: 541 NVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTG 583
           N SHLDYFYALLAVMSAVNF LFLLISK YVYKAEVSDSI++LTDELKKKKSKGLQQTG
Sbjct: 541 NASHLDYFYALLAVMSAVNFGLFLLISKFYVYKAEVSDSIQVLTDELKKKKSKGLQQTG 599

BLAST of Lsi05G017580 vs. NCBI nr
Match: XP_031737789.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucumis sativus] >KAE8650326.1 hypothetical protein Csa_011590 [Cucumis sativus])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.9e-301
Identity = 532/600 (88.67%), Postives = 561/600 (93.50%), Query Frame = 0

Query: 1   MAGAAADQETG---LDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIA 60
           MA AAADQE G   +D+YTKDGTVD KGN VLRSKTG WKACSFI+ YELIERMMF+GIA
Sbjct: 1   MADAAADQEAGVLDVDNYTKDGTVDWKGNAVLRSKTGRWKACSFILGYELIERMMFNGIA 60

Query: 61  ANLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLC--- 120
           ANLIIYLT KLNQGTLTASNNVTNWTGTVWI PILGAYVADA+LGRYRTFFISSL+C   
Sbjct: 61  ANLIIYLTTKLNQGTLTASNNVTNWTGTVWIMPILGAYVADAHLGRYRTFFISSLVCFMA 120

Query: 121 ------------LVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMG 180
                       L PPPC  AI+KENCKQASKLQLA+FFGSLYLLAIASGGTKPNISTMG
Sbjct: 121 MSLLTLTVSIPSLKPPPCSTAISKENCKQASKLQLAMFFGSLYLLAIASGGTKPNISTMG 180

Query: 181 ADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVA 240
           ADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVA
Sbjct: 181 ADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVA 240

Query: 241 ILIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSF 300
           ILIFVVGTPFYRH+LP+GSPFI+MANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNG+F
Sbjct: 241 ILIFVVGTPFYRHKLPSGSPFIKMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGTF 300

Query: 301 KIDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHT 360
           KIDSTPSLRFLNKAA+R  SSDPWR+CTVTEVEETKQMVRMIPIM+CTF+PSTMVAQSHT
Sbjct: 301 KIDSTPSLRFLNKAALRGVSSDPWRICTVTEVEETKQMVRMIPIMVCTFLPSTMVAQSHT 360

Query: 361 LFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITML 420
           LFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLL+ILIYDRIFVKIMQ+VTKNPRGITML
Sbjct: 361 LFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFVKIMQRVTKNPRGITML 420

Query: 421 QRMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVAD 480
           QRMGIGMICH+LVMTVASQVEKHRL+IAA+ GSS ++ QK LPLTIFILLPQFILTGVAD
Sbjct: 421 QRMGIGMICHVLVMTVASQVEKHRLHIAAKYGSS-AEVQKELPLTIFILLPQFILTGVAD 480

Query: 481 AFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNN 540
           AFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLS+FILSKVSEITKRQG GWILNN
Sbjct: 481 AFLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSSFILSKVSEITKRQGNGWILNN 540

Query: 541 LNVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTG 583
           LN SHLDYFYALLAVMS+VN F+FLLISK Y+YKAEVSDSI++LTDELKKKKSKGLQQTG
Sbjct: 541 LNASHLDYFYALLAVMSSVNLFIFLLISKFYIYKAEVSDSIQVLTDELKKKKSKGLQQTG 599

BLAST of Lsi05G017580 vs. NCBI nr
Match: KAA0049221.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.7e-289
Identity = 514/593 (86.68%), Postives = 548/593 (92.41%), Query Frame = 0

Query: 1   MAGAAADQETGLD--DYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAA 60
           MA AAA+QE GLD  +YTKDGTVD KGN VLRSKTG WKACSFI+ YELIERMMF GI++
Sbjct: 1   MADAAANQEAGLDLHNYTKDGTVDWKGNTVLRSKTGRWKACSFILGYELIERMMFHGISS 60

Query: 61  NLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLC---- 120
           NLIIYLT KLNQGTLTASNNVTNW+GTVW  PI+GAYVADA+LGRYRTFFISSL+C    
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWSGTVWTMPIIGAYVADAHLGRYRTFFISSLVCFMAM 120

Query: 121 -----------LVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180
                      L PPPC  +I++ENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA
Sbjct: 121 TLLTAAVSIPSLKPPPCSASISRENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240
           DQFDDFDPKEKAQKLSFFNWWLF+VFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240

Query: 241 LIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFK 300
           LIFVVGTPFYRH+ PNGSPFI MANV VAA WNWRLPLPNDPNQL+ELDLQ+YSKNG+FK
Sbjct: 241 LIFVVGTPFYRHKPPNGSPFITMANVFVAAIWNWRLPLPNDPNQLHELDLQNYSKNGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTL 360
           IDSTPSLRFLNKAAIRR SSDPWR+CTVTEVEETKQMVRMIPIM+C+FIPS MVAQ+HTL
Sbjct: 301 IDSTPSLRFLNKAAIRRVSSDPWRICTVTEVEETKQMVRMIPIMVCSFIPSAMVAQTHTL 360

Query: 361 FIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQ 420
           FIKQGTTL+RSIGSHFKVPPASLYAFVTISMLL+ILIYDRIF+KIMQ+VTKNPRGITMLQ
Sbjct: 361 FIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFLKIMQRVTKNPRGITMLQ 420

Query: 421 RMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADA 480
           RMGIGMICH+LVMTVASQVEKHRL+IAA+ GSS + EQK LPLTIFILLPQFILTGVADA
Sbjct: 421 RMGIGMICHVLVMTVASQVEKHRLHIAAKYGSS-AHEQKELPLTIFILLPQFILTGVADA 480

Query: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNL 540
           FL IA+NEFFYDQAPENMKSLGSSYF TSLGIGNFLSTFILSKVSEITKRQG GWILNNL
Sbjct: 481 FLLIANNEFFYDQAPENMKSLGSSYFTTSLGIGNFLSTFILSKVSEITKRQGNGWILNNL 540

Query: 541 NVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSK 577
           N SHL+YFYALLAVMS+VNFFLFLLISK YVYKAEVSDSI++LTDELKKKKSK
Sbjct: 541 NSSHLNYFYALLAVMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSK 592

BLAST of Lsi05G017580 vs. NCBI nr
Match: XP_011650916.1 (protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KAE8650325.1 hypothetical protein Csa_009670 [Cucumis sativus])

HSP 1 Score: 943.7 bits (2438), Expect = 7.4e-271
Identity = 469/583 (80.45%), Postives = 517/583 (88.68%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           QE+ +D+YT+DGTVD KG P+LRSKTG WKAC FIIVYEL+ER++F G++ANL IYLT K
Sbjct: 14  QESVIDNYTQDGTVDLKGRPLLRSKTGSWKACFFIIVYELLERIVFQGVSANLFIYLTTK 73

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLL-------------- 127
           L+QG +TASNNVTNW G +WI PI GAY+ADA+LGRYRTF ISS +              
Sbjct: 74  LHQGIVTASNNVTNWNGAIWIMPIFGAYIADAHLGRYRTFLISSFIWFTAMSLLTLAVSV 133

Query: 128 -CLVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
             L PPPCL+ I K+NCKQASKLQLAVFFGSLYLL IASGGTKPNISTMGADQFDDF PK
Sbjct: 134 PSLKPPPCLQPITKQNCKQASKLQLAVFFGSLYLLVIASGGTKPNISTMGADQFDDFYPK 193

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           EKAQKLSFFNWW F+ FSGILFASTILVYIQDNVGWSLGYGIPTIG+GVAI+IFVVGTPF
Sbjct: 194 EKAQKLSFFNWWFFTAFSGILFASTILVYIQDNVGWSLGYGIPTIGIGVAIIIFVVGTPF 253

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRHR P+GSPF  +ANVIV A WNWRLP+PNDPNQLYEL++QHYSK G+FKIDSTPSLRF
Sbjct: 254 YRHRPPSGSPFTTIANVIVGAAWNWRLPIPNDPNQLYELEIQHYSKPGNFKIDSTPSLRF 313

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           LNKAAIRR S  PWRLC+VTEVEETKQM+RMIPI+ICTFIP T+ AQ+HTLFIKQGTTLD
Sbjct: 314 LNKAAIRRDSGGPWRLCSVTEVEETKQMLRMIPILICTFIPHTITAQTHTLFIKQGTTLD 373

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           RSIGSHFKVPPASLYAFVTIS+LLSILIYDRIFVKI+++VTKNPRGITMLQRMGIGMICH
Sbjct: 374 RSIGSHFKVPPASLYAFVTISLLLSILIYDRIFVKIVRRVTKNPRGITMLQRMGIGMICH 433

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           +LVM VAS+VEKHRL++AA    S  QEQKVLPLTIF LLPQFILTGVAD+FLQ+A  EF
Sbjct: 434 VLVMVVASRVEKHRLDVAARENGSSPQEQKVLPLTIFTLLPQFILTGVADSFLQVAVQEF 493

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPENMKS G+SY MTSLGIGNFLS+ I+SKVSEITKRQGK WILNNLN SHLDYFY
Sbjct: 494 FYDQAPENMKSFGTSYAMTSLGIGNFLSSLIVSKVSEITKRQGKEWILNNLNASHLDYFY 553

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKS 576
            LLAVMSAVNFFLFL+ISKLY+YKAEVSDSI LLT+ELKKKKS
Sbjct: 554 LLLAVMSAVNFFLFLIISKLYLYKAEVSDSINLLTEELKKKKS 596

BLAST of Lsi05G017580 vs. NCBI nr
Match: KAA0049223.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17335.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 939.1 bits (2426), Expect = 1.8e-269
Identity = 492/599 (82.14%), Postives = 519/599 (86.64%), Query Frame = 0

Query: 1   MAGAAADQETGLD--DYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAA 60
           MA AAADQE GLD   YTKDGTVD K NPVLRSKTG WKACSFI+ YELIERMMF+GI+A
Sbjct: 1   MADAAADQEAGLDLQSYTKDGTVDWKANPVLRSKTGRWKACSFILGYELIERMMFNGISA 60

Query: 61  NLIIYLTIKLNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLC---- 120
           NLIIYLT KLNQGTLTASNNVTNWTGTVWITPILGAYVADA+LGRYRTFFISSL+C    
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWTGTVWITPILGAYVADAHLGRYRTFFISSLVCFMAM 120

Query: 121 -----------LVPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180
                      L PPPC  +I++ENCKQASKLQLA+FFGSLYLLAIASGGTKPNISTMGA
Sbjct: 121 ALLTAAVSIPRLKPPPCSASISRENCKQASKLQLALFFGSLYLLAIASGGTKPNISTMGA 180

Query: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240
           DQFDDFDPKEKAQKLSFFNWWLF+VFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240

Query: 241 LIFVVGTPFYRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFK 300
           LIFVVGTPFYR +LPNGSPFI MA+VIVAA WNWRLPLPNDPNQL+ELDLQHYSKNG+FK
Sbjct: 241 LIFVVGTPFYRLKLPNGSPFITMADVIVAAIWNWRLPLPNDPNQLHELDLQHYSKNGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTL 360
           IDSTPSLRFLNKAAIRR   DP R CTVTEVEETKQMVRMIPIM+CTFIPS MVAQ+HTL
Sbjct: 301 IDSTPSLRFLNKAAIRRVLRDPRRTCTVTEVEETKQMVRMIPIMVCTFIPSAMVAQTHTL 360

Query: 361 FIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQ 420
           FIKQGTTL+RSIGSHFKVPPASLYAFVTISMLL+ILIYDRIFVKIMQ+VTKNPRGITMLQ
Sbjct: 361 FIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFVKIMQRVTKNPRGITMLQ 420

Query: 421 RMGIGMICHILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADA 480
           RMGIGMICH+LVMTVASQVEKHRL+IAA+ GSS + EQK LPLTIFILLPQFILTGVADA
Sbjct: 421 RMGIGMICHVLVMTVASQVEKHRLHIAAKYGSS-AHEQKELPLTIFILLPQFILTGVADA 480

Query: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNL 540
           FLQIASNEFFYDQAPENMKSLGSSYFMTS GIGNFL                        
Sbjct: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSFGIGNFL------------------------ 540

Query: 541 NVSHLDYFYALLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKKKKSKGLQQTG 583
                        +MS+VNFFLFLLISK YVYKAEVSDSI++LTDELKKKKSKGLQQTG
Sbjct: 541 -------------IMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSKGLQQTG 561

BLAST of Lsi05G017580 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 723.8 bits (1867), Expect = 1.1e-208
Identity = 355/579 (61.31%), Postives = 451/579 (77.89%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GNPV RS  G WKACSF++VYE+ ERM + GI++NL IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCL------------ 127
           L+QGT+ +SNNVTNW GT W+TPILGAYV DA LGRY TF IS  +              
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTI 123

Query: 128 --VPPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKE 187
             + PP     N ENC++AS LQLAVFFG+LY LAI +GGTKPNIST+GADQFD FDPKE
Sbjct: 124 PGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPKE 183

Query: 188 KAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFY 247
           K QKLSFFNWW+FS+F G LFA+T+LVY+QDNVGW+LGYG+PT+GL ++I IF++GTPFY
Sbjct: 184 KTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPFY 243

Query: 248 RHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFL 307
           RH+LP GSPF +MA VIVA+      P+ +D    +EL    Y + G+F I  TPSLRFL
Sbjct: 244 RHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRFL 303

Query: 308 NKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDR 367
           ++A+++ G++  W LCT TEVEETKQM+RM+P++  TF+PS M+AQ +TLF+KQGTTLDR
Sbjct: 304 DRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLDR 363

Query: 368 SIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHI 427
            +   F +PPASL  FVT+SML+SI++YDR+FVKI +K T NPRGIT+LQRMGIG+I HI
Sbjct: 364 KVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFHI 423

Query: 428 LVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFF 487
           L+M VAS  E++RL +AA++G  + Q    LPLTIF LLPQF+L G+AD+FL++A  EFF
Sbjct: 424 LIMIVASVTERYRLKVAADHG-LIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEFF 483

Query: 488 YDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFYA 547
           YDQAPE+MKSLG+SY  TSL IGNF+S+F+LS VSEITK++G+GWILNNLN S LDY+Y 
Sbjct: 484 YDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESRLDYYYL 543

Query: 548 LLAVMSAVNFFLFLLISKLYVYKAEVSDSIKLLTDELKK 573
             AV++ VNF LFL++ K YVY+AEV+DS+ +   E+K+
Sbjct: 544 FFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Lsi05G017580 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 695.7 bits (1794), Expect = 3.3e-200
Identity = 340/570 (59.65%), Postives = 443/570 (77.72%), Query Frame = 0

Query: 8   QETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIK 67
           +E G DDYTKDGTVD +GN V RS+TG WKACSF++VYE+ ERM + GI++NL+IY+T K
Sbjct: 4   EEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTK 63

Query: 68  LNQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCLV----------- 127
           L+QGT+ +SNNVTNW GT W+TPILGAYVADA+ GRY TF ISS + L+           
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSL 123

Query: 128 ----PPPCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPK 187
               PP C  A N ENC++AS +QLAVFFG+LY LAI +GGTKPNIST+GADQFD+FDPK
Sbjct: 124 PGLKPPKCSTA-NVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPK 183

Query: 188 EKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPF 247
           +K  K SFFNWW+FS+F G  FA+T+LVY+QDNVGW++GYG+ T+GL  +I IF++GT  
Sbjct: 184 DKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRL 243

Query: 248 YRHRLPNGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRF 307
           YRH+LP GSPF +MA VIVA+    R P+ +D  + YEL    Y+   +F I ST SLRF
Sbjct: 244 YRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRF 303

Query: 308 LNKAAIRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLD 367
           LN+A+++ GS+  WRLCT+TEVEETKQM++M+P++  TF+PS M+AQ  TLFIKQGTTLD
Sbjct: 304 LNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLD 363

Query: 368 RSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICH 427
           R + ++F +PPASL  F T SML+SI+IYDR+FVK M+K+T NPRGIT+LQRMGIGMI H
Sbjct: 364 RRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILH 423

Query: 428 ILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEF 487
           IL+M +AS  E++RL +AAE+G +  Q    +PL+IF LLPQ++L G+ADAF++IA  EF
Sbjct: 424 ILIMIIASITERYRLKVAAEHGLT-HQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEF 483

Query: 488 FYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGKGWILNNLNVSHLDYFY 547
           FYDQAPE+MKSLG+SY  TS+ +G F+S+ +LS VS+ITK+QG+GWI NNLN S LD +Y
Sbjct: 484 FYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRLDNYY 543

Query: 548 ALLAVMSAVNFFLFLLISKLYVYKAEVSDS 563
              AV++ +NF LFL++ + Y Y+A+V+ S
Sbjct: 544 MFFAVLNLLNFILFLVVIRFYEYRADVTQS 570

BLAST of Lsi05G017580 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.9e-157
Identity = 284/560 (50.71%), Postives = 392/560 (70.00%), Query Frame = 0

Query: 15  YTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGTLT 74
           YT+DGTVD +G PVL SKTG W+ACSF++ YE  ERM F GIA+NL+ YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGTVWITPILGAYVADAYLGRYRTFFISSLLCL--------------VPPPC 134
           +  NV NW+G VWITPI GAY+AD+Y+GR+ TF  SSL+ +              + P C
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRPTC 126

Query: 135 LEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLSF 194
              +    C +AS LQ+  F+ SLY +AI +GGTKPNIST GADQFD +  +EK QK+SF
Sbjct: 127 ENGV----CNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 195 FNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPNG 254
           FNWW+FS F G LFA+  LVYIQ+N+GW LGYGIPT+GL V++++F +GTPFYRH++   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 255 SPFIR-MANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAIR 314
               + +  V +AA  N +L  P+D  +LYELD  +Y  NG  ++  TP  RFL+KAAI+
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAIK 306

Query: 315 RGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSIGSHF 374
             S  P   CTVT+VE  K+++ +I I + T IPST+ AQ +TLF+KQGTTLDR IGS+F
Sbjct: 307 TSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGSNF 366

Query: 375 KVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILVMTVA 434
           ++P ASL +FVT+SMLLS+ +YD+ FV  M+K T NPRGIT+LQR+G+G    I+ + +A
Sbjct: 367 QIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIAIA 426

Query: 435 SQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYDQAPE 494
           S VE  R+ +  E    ++   +V+P++IF LLPQ+ L G+ D F  I   EFFYDQ+PE
Sbjct: 427 SAVEVKRMRVIKE--FHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSPE 486

Query: 495 NMKSLGSSYFMTSLGIGNFLSTFILSKVSEIT-KRQGKGWILNNLNVSHLDYFYALLAVM 554
            M+SLG+++F + +G+GNFL++F+++ + +IT K  GK WI NNLN S LDY+Y  L V+
Sbjct: 487 EMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLVVI 546

Query: 555 SAVNFFLFLLISKLYVYKAE 559
           S VN  LF+  +  YVYK++
Sbjct: 547 SIVNMGLFVWAASKYVYKSD 557

BLAST of Lsi05G017580 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 472.6 bits (1215), Expect = 4.5e-133
Identity = 247/565 (43.72%), Postives = 363/565 (64.25%), Query Frame = 0

Query: 13  DDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKLNQGT 72
           D YT+DGTVD   NP  + KTG+WKAC FI+  E  ER+ + G+  NL+ YL  +LNQG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 73  LTASNNVTNWTGTVWITPILGAYVADAYLGRYRT------FFISSLLCLVPPPCLEAINK 132
            TA+NNVTNW+GT +ITP++GA++ADAYLGRY T       ++S +  L     +  +  
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 133 ENCK----QASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKLSFFN 192
            NC       +  Q AVFF +LY++A+ +GG KP +S+ GADQFD+ D  EK +K SFFN
Sbjct: 125 GNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFN 184

Query: 193 WWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLPNGSP 252
           W+ FS+  G L A+T+LV+IQ NVGW  G+G+PT+ + +A+  F  G+ FYR + P GSP
Sbjct: 185 WFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSP 244

Query: 253 FIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAA----- 312
             R+  VIVAA     + +P D + L+E      +  GS K+  T +L+F +KAA     
Sbjct: 245 LTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQS 304

Query: 313 --IRRGSSDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTLDRSI 372
             I+ G  +PWRLC+VT+VEE K ++ ++P+     + +T+ +Q  T+F+ QG T+D+ +
Sbjct: 305 DSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHM 364

Query: 373 GSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMICHILV 432
           G +F++P ASL  F T+S+L    +YD+  + + +K T+N RG T LQRMGIG++  I  
Sbjct: 365 GKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFA 424

Query: 433 MTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNEFFYD 492
           M  A  +E  RL+    +    + +QK + ++IF  +PQ++L G A+ F  I   EFFYD
Sbjct: 425 MITAGVLEVVRLDYVKTHN---AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYD 484

Query: 493 QAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGK-GWILNNLNVSHLDYFYAL 552
           QAP+ M+SL S+  +T++ +GN+LST +++ V +ITK+ GK GWI +NLN  HLDYF+ L
Sbjct: 485 QAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYL 544

Query: 553 LAVMSAVNFFLFLLISKLYVYKAEV 560
           LA +S +NF ++L ISK Y YK  V
Sbjct: 545 LATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Lsi05G017580 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 446.8 bits (1148), Expect = 2.6e-125
Identity = 242/566 (42.76%), Postives = 354/566 (62.54%), Query Frame = 0

Query: 9   ETGLDDYTKDGTVDRKGNPVLRSKTGHWKACSFIIVYELIERMMFSGIAANLIIYLTIKL 68
           E   D YTKDGT+D    P  ++KTG WKAC FI+  E  ER+ + G++ NLI YL  ++
Sbjct: 2   EDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQM 61

Query: 69  NQGTLTASNNVTNWTGTVWITPILGAYVADAYLGRYRT------FFISSLLCLV----PP 128
           N   ++AS +V+NW+GT + TP++GA++ADAYLGRY T       +I+ +  L      P
Sbjct: 62  NMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVP 121

Query: 129 PCLEAINKENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGADQFDDFDPKEKAQKL 188
                 + E C  A+  Q A+ F +LYL+A+ +GG KP +S+ GADQFDD D KEK  K 
Sbjct: 122 GLTPTCSGETC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKS 181

Query: 189 SFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAILIFVVGTPFYRHRLP 248
           SFFNW+ F +  G + AS++LV+IQ NVGW  G G+PT+ + +A++ F  G+ FYR + P
Sbjct: 182 SFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKP 241

Query: 249 NGSPFIRMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGSFKIDSTPSLRFLNKAAI 308
            GSP  RM  VIVA+    ++ +P D + LYE      S  GS K++ T  L F +KAA+
Sbjct: 242 GGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAV 301

Query: 309 RRGS-------SDPWRLCTVTEVEETKQMVRMIPIMICTFIPSTMVAQSHTLFIKQGTTL 368
              S       S  W+LCTVT+VEE K ++R++PI     + +++ +Q  T+F+ QG TL
Sbjct: 302 ETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTL 361

Query: 369 DRSIGSHFKVPPASLYAFVTISMLLSILIYDRIFVKIMQKVTKNPRGITMLQRMGIGMIC 428
           D+ +G +FK+P ASL  F T+S+L    +YD++ V   +K T + RG T LQR+GIG++ 
Sbjct: 362 DQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVI 421

Query: 429 HILVMTVASQVEKHRLNIAAENGSSLSQEQKVLPLTIFILLPQFILTGVADAFLQIASNE 488
            I  M  A  +E  RLN    +       ++ +P+TIF  +PQ+ L G A+ F  I   E
Sbjct: 422 SIFSMVSAGILEVARLNYVQTHN---LYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLE 481

Query: 489 FFYDQAPENMKSLGSSYFMTSLGIGNFLSTFILSKVSEITKRQGK-GWILNNLNVSHLDY 548
           FFYDQAP+ M+SL S+  +T++  GN+LSTF+++ V+++T+  G+ GWI  NLN  HLDY
Sbjct: 482 FFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDY 541

Query: 549 FYALLAVMSAVNFFLFLLISKLYVYK 557
           F+ LLA +S +NF ++L I+K Y YK
Sbjct: 542 FFWLLAGLSFLNFLVYLWIAKWYTYK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FNL71.6e-20761.31Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1[more]
Q9FNL84.6e-19959.65Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1[more]
Q8VZR71.4e-15550.71Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2[more]
Q9M3906.3e-13243.72Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB83.7e-12442.76Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7U6G81.3e-28986.68Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A0A0L9Q03.6e-27180.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134750 PE=3 SV=1[more]
A0A5D3D0T38.8e-27082.14Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1E9F41.9e-26480.41protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431936 ... [more]
A0A6J1CQA15.2e-26276.55protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111013596... [more]
Match NameE-valueIdentityDescription
XP_038874856.11.5e-30890.15protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida][more]
XP_031737789.18.9e-30188.67protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucumis sativus] >KAE8650326.1 hyp... [more]
KAA0049221.12.7e-28986.68protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa][more]
XP_011650916.17.4e-27180.45protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KAE8650325.1 hypothetical protei... [more]
KAA0049223.11.8e-26982.14protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17335.1 protein... [more]
Match NameE-valueIdentityDescription
AT5G46050.11.1e-20861.31peptide transporter 3 [more]
AT5G46040.13.3e-20059.65Major facilitator superfamily protein [more]
AT2G40460.19.9e-15750.71Major facilitator superfamily protein [more]
AT3G54140.14.5e-13343.72peptide transporter 1 [more]
AT5G01180.12.6e-12542.76peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 8..561
e-value: 6.0E-179
score: 597.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 24..551
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 126..519
e-value: 1.0E-94
score: 317.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 19..566
NoneNo IPR availablePANTHERPTHR11654:SF453PROTEIN NRT1/ PTR FAMILY 5.2-RELATEDcoord: 19..566
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 36..555
e-value: 0.0
score: 608.113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G017580.1Lsi05G017580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity