Lsi05G015840 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G015840 vs. ExPASy Swiss-Prot
Match: Q4S6U8 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Tetraodon nigroviridis OX=99883 GN=smek1 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 9.4e-75
Identity = 206/700 (29.43%), Postives = 326/700 (46.57%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQ- 61
           RVKVY LN+D +WDD+GTGHV+  YVER +   L V  E D   LL  +I+P+  Y+KQ 
Sbjct: 7   RVKVYTLNEDRQWDDRGTGHVSSGYVERFKGTSLLVRAESDGSLLLESKINPNTAYQKQQ 66

Query: 62  ---------------------------------------------------EDEAFHSVN 121
                                                              EDE F  ++
Sbjct: 67  DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDVVDESEDERFDDMS 126

Query: 122 SELRELPAVELSTLPLILKTVVESGIGDQMRLTELLL--NDKEFFRKLMNLFRVCEDLEN 181
           S   ELP  EL+ L   L  +V S +   +R  +L L   ++ + RKL++LF VCEDLEN
Sbjct: 127 SPGLELPPCELNRLE-DLAELVASSLPSPLRREKLALAVENEGYIRKLLDLFHVCEDLEN 186

Query: 182 TDGLHMIFKIVRGI--------------------------DDPEVPHTQQFRNFLKEHVV 241
            +GLH I++I++GI                           DP +P  ++ R FL +   
Sbjct: 187 REGLHHIYEIIKGIFLLNRTALFEVMFSDECIMDVIGCLEFDPALPQPRRHREFLTKTAH 246

Query: 242 FKEAIPIKDPPVLSKIHQTYRVGYLKDVVL--ARMLDDSMVANLNSIIHANNAAVVTSLK 301
           FKE IPI DP +  KIHQTYRV Y++D+VL    + +++M++ L+S I  N   +V  L+
Sbjct: 247 FKEVIPISDPELRQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQ 306

Query: 302 DDSTFIQELMA--------------------------------------RDLMNEGIFDI 361
           DD  F+ +L A                                      + L N GI   
Sbjct: 307 DDEKFLTDLFAQLTDEATDDDKRHELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPA 366

Query: 362 VTDVLQSQDKKLVLTGTDILILFLNQDPSLLRTHVVRQ-----EGFPLFGLLVKGMVTDF 421
           +  +L   D ++    TDI    +  +PS++R  V+++     +   L  L+++ M+ D 
Sbjct: 367 LEVILGMDDVQVRAAATDIFSYLVEYNPSMVREFVMQESQQNDDDILLINLIIEHMICDT 426

Query: 422 GDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQ 481
              +    Q + +LR L+D     A +     T    F+ KH   ++     +  TE   
Sbjct: 427 DPELGGAVQLISLLRTLVDPENMLATANKTEKTEFLSFFYKHCMHVLSAPLLANTTEDKP 486

Query: 482 SAKKIQGAGGSLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQR 541
           S    Q +            ++L+ I ELL FCV  H Y IK+  +  +++ +VL+LT  
Sbjct: 487 SKDDFQTS------------QLLALILELLTFCVEHHTYHIKNYIINKDILRRVLVLTAS 546

Query: 542 KEKYLVVAAVRFIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLE 568
           K  +L + A+RF+R ++   DE    Y ++N   +P+I AF+ NGSRYNL+NSA++E+ E
Sbjct: 547 KHAFLALCALRFMRRIIGLKDEFYNRYIMRNFLFEPVIKAFLNNGSRYNLMNSAIIEMFE 606

BLAST of Lsi05G015840 vs. ExPASy Swiss-Prot
Match: Q5SP90 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Danio rerio OX=7955 GN=smek1 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.6e-74
Identity = 206/698 (29.51%), Postives = 326/698 (46.70%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVY LN+D +WDD+GTGHV+  YVER + + L V  E D   LL  +I+P+  Y+KQ+
Sbjct: 7   RVKVYTLNEDRQWDDRGTGHVSSAYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQ 66

Query: 62  D----------------------------------------------------EAFHSVN 121
           D                                                    E F  ++
Sbjct: 67  DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQELIDESEEERFDDMS 126

Query: 122 SELRELPAVELSTLPLILKTVVESGIGDQMRLTE--LLLNDKEFFRKLMNLFRVCEDLEN 181
           S   ELP  ELS L  + + +V S +   +R  +  L L ++ + RKL+ LFRVCEDLEN
Sbjct: 127 SPGLELPPCELSRLEEVAE-LVASSLPSPLRREKLALALENEGYIRKLLELFRVCEDLEN 186

Query: 182 TDGLHMIFKIVRGI--------------------------DDPEVPHTQQFRNFLKEHVV 241
            +GLH ++ I++GI                           DP +P  ++ R FL     
Sbjct: 187 REGLHHLYDIIKGIFLLNRTALFEVMFSEECIMDVIGCLEFDPSLPQPRRHREFLTTTAR 246

Query: 242 FKEAIPIKDPPVLSKIHQTYRVGYLKDVVL--ARMLDDSMVANLNSIIHANNAAVVTSLK 301
           FKE IPI DP +  KIHQTYRV Y++D+VL    + +++M++ L+S I  N   +V  L+
Sbjct: 247 FKEVIPISDPELRQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQ 306

Query: 302 DDSTFIQELMA--------------------------------------RDLMNEGIFDI 361
           DD  F+ EL A                                      + L N GI   
Sbjct: 307 DDEKFLTELFAQLTDEATDDDKRHELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILQA 366

Query: 362 VTDVLQSQDKKLVLTGTDILILFLNQDPSLLRTHVVRQ-----EGFPLFGLLVKGMVTDF 421
           +  +L   D ++    TDI    +  +PS++R  V+++     +   L  L+++ M+ D 
Sbjct: 367 LEVILGMDDVQVRGAATDIFSYLVEYNPSMVREFVMQESQQNDDDILLINLIIEHMICDT 426

Query: 422 GDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQ 481
              +    Q + +LR L+D     A +     T    F+ KH   ++     +  TE   
Sbjct: 427 DPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLSFFYKHCMHVLSAPLLANTTEEKP 486

Query: 482 SAKKIQGAGGSLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQR 541
           S    Q              ++L+ I ELL FCV  H Y IK+  +  +++ +VL+LT  
Sbjct: 487 SKDDFQTC------------QLLALIVELLTFCVEHHTYHIKNYIINKDILRRVLVLTAS 546

Query: 542 KEKYLVVAAVRFIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLE 566
           +  +L + A+RF+R ++   DE    Y ++N   +P++ AF+ NGSRYNL+NSA++E+ E
Sbjct: 547 QHAFLALCALRFMRKIIGLKDEFYNRYIMRNFLFEPVVKAFLNNGSRYNLINSAIIEMFE 606

BLAST of Lsi05G015840 vs. ExPASy Swiss-Prot
Match: Q6INN7 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Xenopus laevis OX=8355 GN=smek1 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.8e-73
Identity = 204/698 (29.23%), Postives = 326/698 (46.70%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVY LN+D +WDD+GTGHV+  YVER + + L V  E D   LL  +I+ +  Y+KQ+
Sbjct: 7   RVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINTNTAYQKQQ 66

Query: 62  D----------------------------------------------------EAFHSVN 121
           D                                                    E F  ++
Sbjct: 67  DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMS 126

Query: 122 SELRELPAVELSTLPLILKTVVESGIGDQMRLTEL--LLNDKEFFRKLMNLFRVCEDLEN 181
           S   ELP+ ELS L  I + VV S +   +R  +L   L ++ + +KL+ LF VCEDLEN
Sbjct: 127 SPGLELPSCELSRLEEIAE-VVASSLPSPLRREKLAVALENEGYLKKLLELFHVCEDLEN 186

Query: 182 TDGLHMIFKIVRGI--------------------------DDPEVPHTQQFRNFLKEHVV 241
            +GLH ++++++GI                           DP +P  ++ R FL +   
Sbjct: 187 IEGLHHLYEVIKGIFLLNRTALFEVMFSEDCMMDIIGCLEYDPSLPTQRKHREFLTKTAK 246

Query: 242 FKEAIPIKDPPVLSKIHQTYRVGYLKDVVL--ARMLDDSMVANLNSIIHANNAAVVTSLK 301
           FKE IPI DP +  KIHQTYRV Y++DVVL    + +++M++ L+S I  N   +V  L+
Sbjct: 247 FKEVIPISDPELKQKIHQTYRVQYIQDVVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQ 306

Query: 302 DDSTFIQELMA--------------------------------------RDLMNEGIFDI 361
           +D  F+ EL A                                      + L N GI   
Sbjct: 307 EDEKFLTELFAHLTDEATDDEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPA 366

Query: 362 VTDVLQSQDKKLVLTGTDILILFLNQDPSLLRTHVVRQ-----EGFPLFGLLVKGMVTDF 421
           +  +L   D ++    TDI    +  +PS++R  V+++     +   L  L+++ M+ D 
Sbjct: 367 LEVILGMDDPQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDT 426

Query: 422 GDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQ 481
              +    Q   +LR L+D     A +     T    F+ KH   ++     +  TE   
Sbjct: 427 DPELGGAVQLTGLLRTLVDPENMLATTNKTEKTEFLGFFYKHCMHVLTAPLLANTTEEKP 486

Query: 482 SAKKIQGAGGSLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQR 541
           S    Q A            ++L+ I ELL FCV  H Y IK+  +  +++ +VL+L   
Sbjct: 487 SKDDFQAA------------QLLALILELLTFCVEHHTYHIKNYIINKDILRRVLVLMSS 546

Query: 542 KEKYLVVAAVRFIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLE 566
              +L + A+RF+R ++   DE    Y VK+   +P++ AF+ NGSRYNL+NSA+LE+ E
Sbjct: 547 MHSFLGLCALRFMRKIVGLKDEFYNRYIVKSFLFEPVVKAFLNNGSRYNLMNSAILEIFE 606

BLAST of Lsi05G015840 vs. ExPASy Swiss-Prot
Match: Q6P2K6 (Serine/threonine-protein phosphatase 4 regulatory subunit 3A OS=Mus musculus OX=10090 GN=Ppp4r3a PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.9e-73
Identity = 203/698 (29.08%), Postives = 326/698 (46.70%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVY LN+D +WDD+GTGHV+  YVER + + L V  E D   LL  +I+P+  Y+KQ+
Sbjct: 7   RVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQ 66

Query: 62  D----------------------------------------------------EAFHSVN 121
           D                                                    E F  ++
Sbjct: 67  DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMS 126

Query: 122 SELRELPAVELSTLPLILKTVVESGIGDQMRLTE--LLLNDKEFFRKLMNLFRVCEDLEN 181
           S   ELP+ ELS L  I + +V S +   +R  +  L L ++ + +KL+ LF VCEDLEN
Sbjct: 127 SPGLELPSCELSRLEEIAE-LVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLEN 186

Query: 182 TDGLHMIFKIVRGI--------------------------DDPEVPHTQQFRNFLKEHVV 241
            +GLH +++I++GI                           DP +   ++ R FL +   
Sbjct: 187 IEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPTLSQPRKHREFLTKTAK 246

Query: 242 FKEAIPIKDPPVLSKIHQTYRVGYLKDVVL--ARMLDDSMVANLNSIIHANNAAVVTSLK 301
           FKE IPI DP +  KIHQTYRV Y++D+VL    + +++M++ L+S I  N   +V  L+
Sbjct: 247 FKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQ 306

Query: 302 DDSTFIQELMA--------------------------------------RDLMNEGIFDI 361
           +D  F+ +L A                                      + L N GI   
Sbjct: 307 EDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPA 366

Query: 362 VTDVLQSQDKKLVLTGTDILILFLNQDPSLLRTHVVRQ-----EGFPLFGLLVKGMVTDF 421
           +  +L   D ++    TDI    +  +PS++R  V+++     +   L  L+++ M+ D 
Sbjct: 367 LEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDDILLINLIIEHMICDT 426

Query: 422 GDNM--HCQFLEILRNLLD---TYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQ 481
              +    Q + +LR L+D     A +     T    F+ KH   ++     +  TE   
Sbjct: 427 DPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKP 486

Query: 482 SAKKIQGAGGSLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQR 541
           S    Q A            ++L+ + ELL FCV  H Y IK+  +  +++ +VL+L   
Sbjct: 487 SKDDFQTA------------QLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMAS 546

Query: 542 KEKYLVVAAVRFIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLE 566
           K  +L + A+RF R ++   DE    Y +K+   +P++ AF+ NGSRYNL+NSA++E+ E
Sbjct: 547 KHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFE 606

BLAST of Lsi05G015840 vs. ExPASy Swiss-Prot
Match: Q6DFT3 (Serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Xenopus tropicalis OX=8364 GN=ppp4r3b PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 8.8e-73
Identity = 206/709 (29.06%), Postives = 327/709 (46.12%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVY LN+D +WDD+GTGHV+  YVER + + L V  E D   LL  +I+P+  Y+KQ+
Sbjct: 7   RVKVYTLNEDRQWDDRGTGHVSSTYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQ 66

Query: 62  D----------------------------------------------------EAFHSV- 121
           D                                                    E F  + 
Sbjct: 67  DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDPIDESEEERFEEMP 126

Query: 122 -NSELRELPAVELSTLPLILKTVVESGIGDQMRLTE--LLLNDKEFFRKLMNLFRVCEDL 181
             S L +LP  ELS L  I   +V S +   +R  +  L L ++ + +KL+ LF+ CE+L
Sbjct: 127 ETSNLIDLPTCELSKLEEI-ADLVTSVLSSPIRREKLALALENEGYIKKLLQLFQTCENL 186

Query: 182 ENTDGLHMIFKIVRGI--------------------------DDPEVPHTQQFRNFLKEH 241
           +NT+GLH +++I+RGI                           DP +   ++ R FL + 
Sbjct: 187 DNTEGLHHLYEIIRGILFLNKAALFEVMFSDECIMDVVGCLEYDPALAQPKRHREFLTKT 246

Query: 242 VVFKEAIPIKDPPVLSKIHQTYRVGYLKDVVL--ARMLDDSMVANLNSIIHANNAAVVTS 301
             FKE IPI D  +  KIHQTYRV Y++DV+L    + +++ ++ L S I  N   +V+ 
Sbjct: 247 AKFKEVIPITDSELRQKIHQTYRVQYIQDVILPTPSVFEENFLSTLTSFIFFNKVEIVSM 306

Query: 302 LKDDSTFIQELMA--------------------------------------RDLMNEGIF 361
           L++D  F+ E+ A                                      + L N GI 
Sbjct: 307 LQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQNRDAFFKTLANLGIL 366

Query: 362 DIVTDVLQSQDKKLVLTGTDILILFLNQDPSLLRTHVVRQ-----EGFPLFGLLVKGMVT 421
             +  V+   D ++    TDI    +   PS++R  V+++     +   L  ++++ M+ 
Sbjct: 367 PALEIVMGMDDLQVRAAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVVIEQMIC 426

Query: 422 DFGDNM--HCQFLEILRNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQS 481
           D    +    Q + +LR L+D   +      T    F        + ++TA       + 
Sbjct: 427 DSDPELGGAVQLMGLLRTLIDPENMLATANKTEKSEFLNFFYNHCMHVLTAPLLANTSED 486

Query: 482 AKKIQGAGGSLGDK-----NGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLL 541
             +     GS+        N    ++L+ I ELL FCV  H Y IK+  +  +++ +VL+
Sbjct: 487 KLEKDAVLGSIKTSTVCPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLI 546

Query: 542 LTQRKEKYLVVAAVRFIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVL 574
           L   K  +L + A+RF+R ++   DE    Y +K    +P+I+A + NG+RYNLLNSA++
Sbjct: 547 LMNSKHTFLALCALRFMRRIIGLKDEFYNRYIIKGNLFEPVINALLDNGTRYNLLNSAII 606

BLAST of Lsi05G015840 vs. ExPASy TrEMBL
Match: A0A0A0L5W2 (SMK-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G166230 PE=4 SV=1)

HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 696/881 (79.00%), Postives = 726/881 (82.41%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAF SVNSEL+ELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDNICNVQRNLHFSNLSNEAFQSVNSELKELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKLMNLFRVCEDLENTDGLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRVCEDLENTDGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           IVRGI                           DPEVPHTQQFRNFLKEHVV+KEAIPIKD
Sbjct: 196 IVRGIVMLNSPQIFDKLFCDELIMDIIGSLEYDPEVPHTQQFRNFLKEHVVYKEAIPIKD 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVL RMLDDSMVANLNS+IHANNAAVVT+LK+DSTFIQELMA
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLVRMLDDSMVANLNSMIHANNAAVVTALKEDSTFIQELMA 315

Query: 302 -----------------------------------------RDLMNEGIFDIVTDVLQSQ 361
                                                    RDLMNEGIFDI+TDVLQSQ
Sbjct: 316 RLRSPSTSCDSKKDLVYFLHEFCCLSKSLQMVQQLRLFSFYRDLMNEGIFDIITDVLQSQ 375

Query: 362 DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILR 421
           DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPL GLLVKGMVTDFGDNMHCQFLEILR
Sbjct: 376 DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLLGLLVKGMVTDFGDNMHCQFLEILR 435

Query: 422 NLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGV 481
           +LLDTYALSGVQRD+IIEIFYEKHLG LIDIIT SCPTEGFQSAKKI+GAGGSLGDKNGV
Sbjct: 436 SLLDTYALSGVQRDSIIEIFYEKHLGHLIDIITTSCPTEGFQSAKKIEGAGGSLGDKNGV 495

Query: 482 KPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLS 541
           KPEILSNICELLCFCV QHPYRIKSNFLLNN+IDKV+LLT+RKEKYLVVAAVRFIRTVLS
Sbjct: 496 KPEILSNICELLCFCVQQHPYRIKSNFLLNNMIDKVVLLTRRKEKYLVVAAVRFIRTVLS 555

Query: 542 RHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSF 601
           RHDEHLINYF+KNGT+KPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYI+DSF
Sbjct: 556 RHDEHLINYFIKNGTLKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIVDSF 615

Query: 602 WNQLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSD 661
           WNQLS+F+NL SIQALK+KYEQCLENCEMKG SNTSDPRK+NDERA EKEEEDYFNEDSD
Sbjct: 616 WNQLSQFKNLSSIQALKIKYEQCLENCEMKGISNTSDPRKKNDERAPEKEEEDYFNEDSD 675

Query: 662 EEDTASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPP 721
           EEDT  ASVAPKQNVE Q A  NGVTTN P V      S+RSGGLVDYDDDEDDEDYRPP
Sbjct: 676 EEDT--ASVAPKQNVESQPALSNGVTTNCPSV------SYRSGGLVDYDDDEDDEDYRPP 735

Query: 722 HRKQPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAV 770
            RKQPDTSDE NE+MEALRQKRKSAPQEE E+VKKQRLIKK KPKDGVF SLCTTLSQAV
Sbjct: 736 QRKQPDTSDEGNEMMEALRQKRKSAPQEEPELVKKQRLIKKLKPKDGVFVSLCTTLSQAV 795

BLAST of Lsi05G015840 vs. ExPASy TrEMBL
Match: A0A1S3AY73 (serine/threonine-protein phosphatase 4 regulatory subunit 3 OS=Cucumis melo OX=3656 GN=LOC103483885 PE=4 SV=1)

HSP 1 Score: 1250.7 bits (3235), Expect = 0.0e+00
Identity = 689/881 (78.21%), Postives = 718/881 (81.50%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAF SVNSEL+ELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDNICNVQRNLHFSNLSNEAFQSVNSELKELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKLMNLFRVCEDLENT+GLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRVCEDLENTEGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           IVRGI                           DPEVPH QQFRNFLKEHVV+KEAIPIKD
Sbjct: 196 IVRGIVLLNSPQIFDKLFCDELIMDIIGSLEYDPEVPHPQQFRNFLKEHVVYKEAIPIKD 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVLAR+LDDSMVANLNS+IHANNAAVVT+LK+DSTFIQELMA
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLARILDDSMVANLNSMIHANNAAVVTALKEDSTFIQELMA 315

Query: 302 -----------------------------------------RDLMNEGIFDIVTDVLQSQ 361
                                                    RDLMNEGIFDI+TDVLQSQ
Sbjct: 316 RLRSPSTSCDSKKDLVYFLHEFCCLSKSLQMVQQLRLFSFYRDLMNEGIFDIITDVLQSQ 375

Query: 362 DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILR 421
           DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPL GLLVKGMVTDFGDNMHCQFLEILR
Sbjct: 376 DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLLGLLVKGMVTDFGDNMHCQFLEILR 435

Query: 422 NLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGV 481
           +LLDTYALSGVQRD+IIEIFYEKHLG LIDIIT SCPTEGFQSAKKI+GAGGSLGDKNGV
Sbjct: 436 SLLDTYALSGVQRDSIIEIFYEKHLGHLIDIITTSCPTEGFQSAKKIEGAGGSLGDKNGV 495

Query: 482 KPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLS 541
           KPEILSNICELLCFCV QHPYRIKSNFLLNN+IDKV+LLT+RKEKYLVVAAVRFIRTVLS
Sbjct: 496 KPEILSNICELLCFCVQQHPYRIKSNFLLNNMIDKVVLLTRRKEKYLVVAAVRFIRTVLS 555

Query: 542 RHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSF 601
           RHDEHLINYF+KNGT+KPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSF
Sbjct: 556 RHDEHLINYFIKNGTLKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSF 615

Query: 602 WNQLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSD 661
           WNQLS+FENL SIQALKVKYEQ LENCE+KG  NTSDPRKRNDERA EKEEEDYFNEDSD
Sbjct: 616 WNQLSQFENLSSIQALKVKYEQSLENCEVKGILNTSDPRKRNDERAPEKEEEDYFNEDSD 675

Query: 662 EEDTASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPP 721
           EEDT  ASVAPKQNVE Q A  NGVTTN P V      S RSGGLVDYDDDEDDEDYRPP
Sbjct: 676 EEDT--ASVAPKQNVESQPALSNGVTTNRPSV------SLRSGGLVDYDDDEDDEDYRPP 735

Query: 722 HRKQPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAV 770
            RKQPDT+DE NE+MEALRQKRKSAPQEE  +VKKQRLIKK KPKDGVF SLCTTLSQAV
Sbjct: 736 QRKQPDTTDEGNEMMEALRQKRKSAPQEEPALVKKQRLIKKCKPKDGVFVSLCTTLSQAV 795

BLAST of Lsi05G015840 vs. ExPASy TrEMBL
Match: A0A6J1GTW5 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457499 PE=4 SV=1)

HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 683/879 (77.70%), Postives = 717/879 (81.57%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDY+ERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAFHSVNSELRELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLSNEAFHSVNSELRELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKL NLFRVCEDLEN DGLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRVCEDLENIDGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           I RGI                           DPEVPH Q+FRNFLKEHVVFKEAIPIK+
Sbjct: 196 IFRGIVLLNSPQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNFLKEHVVFKEAIPIKN 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVLARM+DDSMVA+LNSIIHANNA VVTSLKDDSTFIQEL A
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVVTSLKDDSTFIQELFA 315

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDLMNEGIFDI+TDVLQSQDKK
Sbjct: 316 RLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEGIFDIITDVLQSQDKK 375

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
            VLTGTDILILFLNQDP+LLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL
Sbjct: 376 FVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 435

Query: 422 DTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKPE 481
           DTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+SAKKI+GA   LGDKNGVKPE
Sbjct: 436 DTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIEGAVEGLGDKNGVKPE 495

Query: 482 ILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRHD 541
           ILSNICELLCFCVLQHPYRIKSNFLLNN+IDKV+LLTQR+EKYLVVAAVRF+RT+LSRHD
Sbjct: 496 ILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLVVAAVRFVRTILSRHD 555

Query: 542 EHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 601
           EHLINYFVKN T+KPIIDAFVANG+RYNLLNS VL+LLEFIR ENLKLL+RYIIDSFWNQ
Sbjct: 556 EHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTENLKLLIRYIIDSFWNQ 615

Query: 602 LSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEED 661
           LS+FENLPSIQALKVKYEQCLEN EMKG   TSDPRKRNDERALEKEEEDYFNEDSDEED
Sbjct: 616 LSQFENLPSIQALKVKYEQCLENYEMKGALETSDPRKRNDERALEKEEEDYFNEDSDEED 675

Query: 662 TASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPPHRK 721
           TASA  APKQNV+ Q A  NGVTTNYP      L S RSGGLVDYDDDEDDEDYRPP R 
Sbjct: 676 TASA--APKQNVQSQPALCNGVTTNYP------LVSLRSGGLVDYDDDEDDEDYRPPQRD 735

Query: 722 QPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAVLPG 770
           QPD+SDEDNE+ME+L QKRK APQEE +MVKKQ L KK KPKDGV ASLCTTLSQA+LPG
Sbjct: 736 QPDSSDEDNEMMESLGQKRKLAPQEEPQMVKKQLLSKKYKPKDGVLASLCTTLSQAILPG 795

BLAST of Lsi05G015840 vs. ExPASy TrEMBL
Match: A0A6J1GTS7 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457499 PE=4 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 683/879 (77.70%), Postives = 716/879 (81.46%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDY+ERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAFHSVNSELRELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLSNEAFHSVNSELRELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND +FFRKL NLFRVCEDLEN DGLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLND-DFFRKLTNLFRVCEDLENIDGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           I RGI                           DPEVPH Q+FRNFLKEHVVFKEAIPIK+
Sbjct: 196 IFRGIVLLNSPQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNFLKEHVVFKEAIPIKN 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVLARM+DDSMVA+LNSIIHANNA VVTSLKDDSTFIQEL A
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVVTSLKDDSTFIQELFA 315

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDLMNEGIFDI+TDVLQSQDKK
Sbjct: 316 RLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEGIFDIITDVLQSQDKK 375

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
            VLTGTDILILFLNQDP+LLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL
Sbjct: 376 FVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 435

Query: 422 DTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKPE 481
           DTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+SAKKI+GA   LGDKNGVKPE
Sbjct: 436 DTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIEGAVEGLGDKNGVKPE 495

Query: 482 ILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRHD 541
           ILSNICELLCFCVLQHPYRIKSNFLLNN+IDKV+LLTQR+EKYLVVAAVRF+RT+LSRHD
Sbjct: 496 ILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLVVAAVRFVRTILSRHD 555

Query: 542 EHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 601
           EHLINYFVKN T+KPIIDAFVANG+RYNLLNS VL+LLEFIR ENLKLL+RYIIDSFWNQ
Sbjct: 556 EHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTENLKLLIRYIIDSFWNQ 615

Query: 602 LSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEED 661
           LS+FENLPSIQALKVKYEQCLEN EMKG   TSDPRKRNDERALEKEEEDYFNEDSDEED
Sbjct: 616 LSQFENLPSIQALKVKYEQCLENYEMKGALETSDPRKRNDERALEKEEEDYFNEDSDEED 675

Query: 662 TASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPPHRK 721
           TASA  APKQNV+ Q A  NGVTTNYP      L S RSGGLVDYDDDEDDEDYRPP R 
Sbjct: 676 TASA--APKQNVQSQPALCNGVTTNYP------LVSLRSGGLVDYDDDEDDEDYRPPQRD 735

Query: 722 QPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAVLPG 770
           QPD+SDEDNE+ME+L QKRK APQEE +MVKKQ L KK KPKDGV ASLCTTLSQA+LPG
Sbjct: 736 QPDSSDEDNEMMESLGQKRKLAPQEEPQMVKKQLLSKKYKPKDGVLASLCTTLSQAILPG 795

BLAST of Lsi05G015840 vs. ExPASy TrEMBL
Match: A0A6J1IXB5 (serine/threonine-protein phosphatase 4 regulatory subunit 3A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479339 PE=4 SV=1)

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 678/877 (77.31%), Postives = 713/877 (81.30%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDY+ERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAFHSVNSELRELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLSNEAFHSVNSELRELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKL NLFRVCEDLEN DGLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRVCEDLENIDGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           I RGI                           DPEVPH Q+FRNFLKEHVVFKEAIPIK+
Sbjct: 196 IFRGIVLLNSQQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNFLKEHVVFKEAIPIKN 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVLARM+DDSMVA+LNSIIHANNA VVTSLKDDSTFIQEL A
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVVTSLKDDSTFIQELFA 315

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDLMNEGIFDI+TDVLQSQDKK
Sbjct: 316 RLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEGIFDIITDVLQSQDKK 375

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
            VLTGTDILILFLNQDP+LLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL
Sbjct: 376 FVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 435

Query: 422 DTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKPE 481
           DTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+SAKKI+GA   LGDKNGVKPE
Sbjct: 436 DTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIEGAVEGLGDKNGVKPE 495

Query: 482 ILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRHD 541
           ILSNICELLCFCVLQHPYRIKSNFLLNN+IDKV+LLTQR+EKYLVVAAVRF+RTVLSRHD
Sbjct: 496 ILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLVVAAVRFVRTVLSRHD 555

Query: 542 EHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 601
           EHLINYFVKN T+KPIIDAFVANG+RYNLLNS VL+LLEFIR ENLKLL+RYIIDSFWNQ
Sbjct: 556 EHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTENLKLLIRYIIDSFWNQ 615

Query: 602 LSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEED 661
           LS+FENLPSIQALKVKYEQCLEN EMKGT  TSDPRKRNDERALEKEEEDYFNEDSDE D
Sbjct: 616 LSQFENLPSIQALKVKYEQCLENYEMKGTLETSDPRKRNDERALEKEEEDYFNEDSDEVD 675

Query: 662 TASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPPHRK 721
           TASA  APKQNV+ Q A  NG TTNYP      L S RSGGLVDYDDDEDDEDYRPP R 
Sbjct: 676 TASA--APKQNVQSQPALSNGGTTNYP------LVSLRSGGLVDYDDDEDDEDYRPPQRD 735

Query: 722 QPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAVLPG 770
           QPD+SDEDNE+ME+L QKRK APQEE +M+KKQ L KK KPKDGV ASLC+TLSQA+LPG
Sbjct: 736 QPDSSDEDNEMMESLGQKRKLAPQEEPQMIKKQLLSKKYKPKDGVLASLCSTLSQAILPG 795

BLAST of Lsi05G015840 vs. NCBI nr
Match: XP_031738345.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 696/878 (79.27%), Postives = 726/878 (82.69%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAF SVNSEL+ELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDNICNVQRNLHFSNLSNEAFQSVNSELKELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKLMNLFRVCEDLENTDGLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRVCEDLENTDGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           IVRGI                           DPEVPHTQQFRNFLKEHVV+KEAIPIKD
Sbjct: 196 IVRGIVMLNSPQIFDKLFCDELIMDIIGSLEYDPEVPHTQQFRNFLKEHVVYKEAIPIKD 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVL RMLDDSMVANLNS+IHANNAAVVT+LK+DSTFIQELMA
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLVRMLDDSMVANLNSMIHANNAAVVTALKEDSTFIQELMA 315

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDLMNEGIFDI+TDVLQSQDKK
Sbjct: 316 RLRSPSTSCDSKKDLVYFLHEFCCLSKSLQMVQQLRLFRDLMNEGIFDIITDVLQSQDKK 375

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
           LVLTGTDILILFLNQDPSLLRTHVVRQEGFPL GLLVKGMVTDFGDNMHCQFLEILR+LL
Sbjct: 376 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLLGLLVKGMVTDFGDNMHCQFLEILRSLL 435

Query: 422 DTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKPE 481
           DTYALSGVQRD+IIEIFYEKHLG LIDIIT SCPTEGFQSAKKI+GAGGSLGDKNGVKPE
Sbjct: 436 DTYALSGVQRDSIIEIFYEKHLGHLIDIITTSCPTEGFQSAKKIEGAGGSLGDKNGVKPE 495

Query: 482 ILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRHD 541
           ILSNICELLCFCV QHPYRIKSNFLLNN+IDKV+LLT+RKEKYLVVAAVRFIRTVLSRHD
Sbjct: 496 ILSNICELLCFCVQQHPYRIKSNFLLNNMIDKVVLLTRRKEKYLVVAAVRFIRTVLSRHD 555

Query: 542 EHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 601
           EHLINYF+KNGT+KPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYI+DSFWNQ
Sbjct: 556 EHLINYFIKNGTLKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIVDSFWNQ 615

Query: 602 LSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEED 661
           LS+F+NL SIQALK+KYEQCLENCEMKG SNTSDPRK+NDERA EKEEEDYFNEDSDEED
Sbjct: 616 LSQFKNLSSIQALKIKYEQCLENCEMKGISNTSDPRKKNDERAPEKEEEDYFNEDSDEED 675

Query: 662 TASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPPHRK 721
           T  ASVAPKQNVE Q A  NGVTTN P V      S+RSGGLVDYDDDEDDEDYRPP RK
Sbjct: 676 T--ASVAPKQNVESQPALSNGVTTNCPSV------SYRSGGLVDYDDDEDDEDYRPPQRK 735

Query: 722 QPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAVLPG 770
           QPDTSDE NE+MEALRQKRKSAPQEE E+VKKQRLIKK KPKDGVF SLCTTLSQAVLPG
Sbjct: 736 QPDTSDEGNEMMEALRQKRKSAPQEEPELVKKQRLIKKLKPKDGVFVSLCTTLSQAVLPG 795

BLAST of Lsi05G015840 vs. NCBI nr
Match: XP_004134241.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3 isoform X1 [Cucumis sativus] >KGN57158.1 hypothetical protein Csa_010320 [Cucumis sativus])

HSP 1 Score: 1272.7 bits (3292), Expect = 0.0e+00
Identity = 696/881 (79.00%), Postives = 726/881 (82.41%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAF SVNSEL+ELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDNICNVQRNLHFSNLSNEAFQSVNSELKELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKLMNLFRVCEDLENTDGLHMIFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLMNLFRVCEDLENTDGLHMIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           IVRGI                           DPEVPHTQQFRNFLKEHVV+KEAIPIKD
Sbjct: 196 IVRGIVMLNSPQIFDKLFCDELIMDIIGSLEYDPEVPHTQQFRNFLKEHVVYKEAIPIKD 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVL RMLDDSMVANLNS+IHANNAAVVT+LK+DSTFIQELMA
Sbjct: 256 PLVLSKIHQTYRVGYLKDVVLVRMLDDSMVANLNSMIHANNAAVVTALKEDSTFIQELMA 315

Query: 302 -----------------------------------------RDLMNEGIFDIVTDVLQSQ 361
                                                    RDLMNEGIFDI+TDVLQSQ
Sbjct: 316 RLRSPSTSCDSKKDLVYFLHEFCCLSKSLQMVQQLRLFSFYRDLMNEGIFDIITDVLQSQ 375

Query: 362 DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILR 421
           DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPL GLLVKGMVTDFGDNMHCQFLEILR
Sbjct: 376 DKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLLGLLVKGMVTDFGDNMHCQFLEILR 435

Query: 422 NLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGV 481
           +LLDTYALSGVQRD+IIEIFYEKHLG LIDIIT SCPTEGFQSAKKI+GAGGSLGDKNGV
Sbjct: 436 SLLDTYALSGVQRDSIIEIFYEKHLGHLIDIITTSCPTEGFQSAKKIEGAGGSLGDKNGV 495

Query: 482 KPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLS 541
           KPEILSNICELLCFCV QHPYRIKSNFLLNN+IDKV+LLT+RKEKYLVVAAVRFIRTVLS
Sbjct: 496 KPEILSNICELLCFCVQQHPYRIKSNFLLNNMIDKVVLLTRRKEKYLVVAAVRFIRTVLS 555

Query: 542 RHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSF 601
           RHDEHLINYF+KNGT+KPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYI+DSF
Sbjct: 556 RHDEHLINYFIKNGTLKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIVDSF 615

Query: 602 WNQLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSD 661
           WNQLS+F+NL SIQALK+KYEQCLENCEMKG SNTSDPRK+NDERA EKEEEDYFNEDSD
Sbjct: 616 WNQLSQFKNLSSIQALKIKYEQCLENCEMKGISNTSDPRKKNDERAPEKEEEDYFNEDSD 675

Query: 662 EEDTASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPP 721
           EEDT  ASVAPKQNVE Q A  NGVTTN P V      S+RSGGLVDYDDDEDDEDYRPP
Sbjct: 676 EEDT--ASVAPKQNVESQPALSNGVTTNCPSV------SYRSGGLVDYDDDEDDEDYRPP 735

Query: 722 HRKQPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAV 770
            RKQPDTSDE NE+MEALRQKRKSAPQEE E+VKKQRLIKK KPKDGVF SLCTTLSQAV
Sbjct: 736 QRKQPDTSDEGNEMMEALRQKRKSAPQEEPELVKKQRLIKKLKPKDGVFVSLCTTLSQAV 795

BLAST of Lsi05G015840 vs. NCBI nr
Match: XP_038881877.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1265.8 bits (3274), Expect = 0.0e+00
Identity = 695/879 (79.07%), Postives = 718/879 (81.68%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAFHSVNSELRELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDNICNVQRNLQFSNLSNEAFHSVNSELRELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQM+LTELLLND+EF +KL +LFRVCEDLENT+GLH IFK
Sbjct: 136 AVELSTLPLILKTVVESGIADQMKLTELLLNDQEFLKKLTDLFRVCEDLENTNGLHTIFK 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           IVRGI                           DPEVPHT+QFRNFLKEHVVFKEAIPIKD
Sbjct: 196 IVRGIVMLNSPQIFDKLFCDELIMDVIGSLEYDPEVPHTRQFRNFLKEHVVFKEAIPIKD 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVL RMLDDSMVANLNSIIHANNA VVTSLKDDSTFIQEL+A
Sbjct: 256 PHVLSKIHQTYRVGYLKDVVLTRMLDDSMVANLNSIIHANNAVVVTSLKDDSTFIQELLA 315

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDL++EGIFDI+TDVLQSQDKK
Sbjct: 316 RLRSSSTSDNSKKDLVYFLHEFCCSSKSLQMVQQLRLFRDLVSEGIFDIITDVLQSQDKK 375

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
           LVLTGTDILILFLNQDP+LLRTHVV+QEG PLFGLLVKGMVTDFGDNMHCQFLEILRNLL
Sbjct: 376 LVLTGTDILILFLNQDPTLLRTHVVQQEGSPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 435

Query: 422 DTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKPE 481
           DTYAL GVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKI+GAGGSLGD N VKPE
Sbjct: 436 DTYALPGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIEGAGGSLGDTNSVKPE 495

Query: 482 ILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRHD 541
           ILSNICELLCFC+LQHPYRIKSNFLLNNVIDKV+LLTQR+EKYLVVAAVRFIRTVLSRHD
Sbjct: 496 ILSNICELLCFCILQHPYRIKSNFLLNNVIDKVVLLTQRREKYLVVAAVRFIRTVLSRHD 555

Query: 542 EHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 601
           EHLINYFVKN T+KPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ
Sbjct: 556 EHLINYFVKNSTLKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 615

Query: 602 LSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEED 661
           LSKFENL SIQALKVKYEQCLENCEMKGT NTSDPRKRNDERA EKEEEDYFN DSDEED
Sbjct: 616 LSKFENLSSIQALKVKYEQCLENCEMKGTLNTSDPRKRNDERAPEKEEEDYFNGDSDEED 675

Query: 662 TASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPPHRK 721
           TASASVAPKQNVE   A  NGV TNYPP       S RSGGLVDYDDDEDDEDYRPP RK
Sbjct: 676 TASASVAPKQNVESHPALSNGVATNYPPA------SLRSGGLVDYDDDEDDEDYRPPQRK 735

Query: 722 QPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAVLPG 770
           QPDTSDEDNEVMEALR KRK A QEE EM KKQR IKK KPKDGVFASLCTTLSQAVLPG
Sbjct: 736 QPDTSDEDNEVMEALRHKRKLASQEEPEMAKKQRPIKKCKPKDGVFASLCTTLSQAVLPG 795

BLAST of Lsi05G015840 vs. NCBI nr
Match: XP_038881870.1 (serine/threonine-protein phosphatase 4 regulatory subunit 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 695/883 (78.71%), Postives = 718/883 (81.31%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 16  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAFHSVNSELRELP
Sbjct: 76  DTIISWRDPEYSTELALSFQEATGCSFIWDNICNVQRNLQFSNLSNEAFHSVNSELRELP 135

Query: 122 AVELSTLPLILK----TVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLH 181
           AVELSTLPLILK    TVVESGI DQM+LTELLLND+EF +KL +LFRVCEDLENT+GLH
Sbjct: 136 AVELSTLPLILKVLADTVVESGIADQMKLTELLLNDQEFLKKLTDLFRVCEDLENTNGLH 195

Query: 182 MIFKIVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAI 241
            IFKIVRGI                           DPEVPHT+QFRNFLKEHVVFKEAI
Sbjct: 196 TIFKIVRGIVMLNSPQIFDKLFCDELIMDVIGSLEYDPEVPHTRQFRNFLKEHVVFKEAI 255

Query: 242 PIKDPPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQ 301
           PIKDP VLSKIHQTYRVGYLKDVVL RMLDDSMVANLNSIIHANNA VVTSLKDDSTFIQ
Sbjct: 256 PIKDPHVLSKIHQTYRVGYLKDVVLTRMLDDSMVANLNSIIHANNAVVVTSLKDDSTFIQ 315

Query: 302 ELMA--------------------------------------RDLMNEGIFDIVTDVLQS 361
           EL+A                                      RDL++EGIFDI+TDVLQS
Sbjct: 316 ELLARLRSSSTSDNSKKDLVYFLHEFCCSSKSLQMVQQLRLFRDLVSEGIFDIITDVLQS 375

Query: 362 QDKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEIL 421
           QDKKLVLTGTDILILFLNQDP+LLRTHVV+QEG PLFGLLVKGMVTDFGDNMHCQFLEIL
Sbjct: 376 QDKKLVLTGTDILILFLNQDPTLLRTHVVQQEGSPLFGLLVKGMVTDFGDNMHCQFLEIL 435

Query: 422 RNLLDTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNG 481
           RNLLDTYAL GVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKI+GAGGSLGD N 
Sbjct: 436 RNLLDTYALPGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIEGAGGSLGDTNS 495

Query: 482 VKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVL 541
           VKPEILSNICELLCFC+LQHPYRIKSNFLLNNVIDKV+LLTQR+EKYLVVAAVRFIRTVL
Sbjct: 496 VKPEILSNICELLCFCILQHPYRIKSNFLLNNVIDKVVLLTQRREKYLVVAAVRFIRTVL 555

Query: 542 SRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDS 601
           SRHDEHLINYFVKN T+KPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDS
Sbjct: 556 SRHDEHLINYFVKNSTLKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDS 615

Query: 602 FWNQLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDS 661
           FWNQLSKFENL SIQALKVKYEQCLENCEMKGT NTSDPRKRNDERA EKEEEDYFN DS
Sbjct: 616 FWNQLSKFENLSSIQALKVKYEQCLENCEMKGTLNTSDPRKRNDERAPEKEEEDYFNGDS 675

Query: 662 DEEDTASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRP 721
           DEEDTASASVAPKQNVE   A  NGV TNYPP       S RSGGLVDYDDDEDDEDYRP
Sbjct: 676 DEEDTASASVAPKQNVESHPALSNGVATNYPPA------SLRSGGLVDYDDDEDDEDYRP 735

Query: 722 PHRKQPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQA 770
           P RKQPDTSDEDNEVMEALR KRK A QEE EM KKQR IKK KPKDGVFASLCTTLSQA
Sbjct: 736 PQRKQPDTSDEDNEVMEALRHKRKLASQEEPEMAKKQRPIKKCKPKDGVFASLCTTLSQA 795

BLAST of Lsi05G015840 vs. NCBI nr
Match: KAG7018577.1 (Serine/threonine-protein phosphatase 4 regulatory subunit 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 685/879 (77.93%), Postives = 718/879 (81.68%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVYRLNDDGKWDDQGTGHVTVDY+ERSEELGLFVIDEEDNETLLLHRIS DDIYRKQE
Sbjct: 28  RVKVYRLNDDGKWDDQGTGHVTVDYMERSEELGLFVIDEEDNETLLLHRISSDDIYRKQE 87

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             EAFHSVNSELRELP
Sbjct: 88  DTIISWRDPEYSTELALSFQEATGCSFIWDSICSVQRNLHFSNLSNEAFHSVNSELRELP 147

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVELSTLPLILKTVVESGI DQMRLTELLLND++FFRKL NLFRVCEDLEN DGLHMIFK
Sbjct: 148 AVELSTLPLILKTVVESGIADQMRLTELLLNDQDFFRKLTNLFRVCEDLENIDGLHMIFK 207

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           I RGI                           DPEVPH Q+FRNFLKEHVVFKEAIPIK+
Sbjct: 208 IFRGIVLLNSPQIFEKIFSDELIMDIIGSLEYDPEVPHPQKFRNFLKEHVVFKEAIPIKN 267

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYRVGYLKDVVLARM+DDSMVA+LNSIIHANNA VVTSLKDDSTFIQEL A
Sbjct: 268 PLVLSKIHQTYRVGYLKDVVLARMMDDSMVASLNSIIHANNATVVTSLKDDSTFIQELFA 327

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDLMNEGIFDI+TDVLQSQDKK
Sbjct: 328 RLRSPSTSDDSKKDLVYFLHEFCCLSKSLQMVHQLRLFRDLMNEGIFDIITDVLQSQDKK 387

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
            VLTGTDILILFLNQDP+LLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL
Sbjct: 388 FVLTGTDILILFLNQDPNLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 447

Query: 422 DTYALSGVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKPE 481
           DTYALSGVQRDT+IEIFYEKHLGQLIDIITASCPTE F+SAKKI+GA   LGDKNGVKPE
Sbjct: 448 DTYALSGVQRDTVIEIFYEKHLGQLIDIITASCPTESFKSAKKIEGAVEGLGDKNGVKPE 507

Query: 482 ILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRHD 541
           ILSNICELLCFCVLQHPYRIKSNFLLNN+IDKV+LLTQR+EKYLVVAAVRF+RTVLSRHD
Sbjct: 508 ILSNICELLCFCVLQHPYRIKSNFLLNNMIDKVVLLTQRREKYLVVAAVRFVRTVLSRHD 567

Query: 542 EHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWNQ 601
           EHLINYFVKN T+KPIIDAFVANG+RYNLLNS VL+LLEFIR ENLKLL+RYIIDSFWNQ
Sbjct: 568 EHLINYFVKNSTLKPIIDAFVANGNRYNLLNSVVLDLLEFIRTENLKLLIRYIIDSFWNQ 627

Query: 602 LSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEED 661
           LS+FENLPSIQALKVKYEQCLEN EMKGT  TSDPRKRNDERALEKEEEDYFNEDSDEED
Sbjct: 628 LSQFENLPSIQALKVKYEQCLENYEMKGTLETSDPRKRNDERALEKEEEDYFNEDSDEED 687

Query: 662 TASASVAPKQNVECQRAFPNGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDEDYRPPHRK 721
           TASA  APKQNV+ QRA  NGVTTNYP      L S RSGGLVDYDDDEDDEDYRPP R 
Sbjct: 688 TASA--APKQNVQSQRALCNGVTTNYP------LVSLRSGGLVDYDDDEDDEDYRPPQRD 747

Query: 722 QPDTSDEDNEVMEALRQKRKSAPQEEHEMVKKQRLIKKNKPKDGVFASLCTTLSQAVLPG 770
           QPD+SDEDNE+ME+L QKRK APQEE +MVKKQ L KK KPKDGV ASLCTTLSQA+LPG
Sbjct: 748 QPDSSDEDNEMMESLGQKRKLAPQEEPQMVKKQLLSKKYKPKDGVLASLCTTLSQAILPG 807

BLAST of Lsi05G015840 vs. TAIR 10
Match: AT3G06670.1 (binding )

HSP 1 Score: 828.2 bits (2138), Expect = 5.6e-240
Identity = 493/884 (55.77%), Postives = 596/884 (67.42%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVY LN+DGKWDD+GTGHV++D+VERSEEL L VIDEEDNETLL+H I+P+DIYRKQE
Sbjct: 16  RVKVYHLNEDGKWDDRGTGHVSIDFVERSEELSLCVIDEEDNETLLVHPINPEDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             E FHS+NSELRELP
Sbjct: 76  DTIISWRDPERSTELALSFQETAGCSYVWDQICTMQRNLHFSSLNSETFHSLNSELRELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVEL+TLPLILK V ESGI DQMRLTEL+L D +FFR LM +F++CEDLEN DGLHMIF 
Sbjct: 136 AVELTTLPLILKIVTESGITDQMRLTELILKDHDFFRNLMGVFKICEDLENVDGLHMIFN 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKD 241
           IV+GI                           DP VPH+Q  RNFLKEHVVFKEAIPIKD
Sbjct: 196 IVKGIILLNSSQILEKIFGDELIMEIIGCLEYDPGVPHSQHHRNFLKEHVVFKEAIPIKD 255

Query: 242 PPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMA 301
           P VLSKIHQTYR+GYLKDVVLAR+LDD++VANLNS+IHANNA VV+ LKDDSTFIQEL A
Sbjct: 256 PLVLSKIHQTYRIGYLKDVVLARVLDDAIVANLNSVIHANNAIVVSLLKDDSTFIQELFA 315

Query: 302 --------------------------------------RDLMNEGIFDIVTDVLQSQDKK 361
                                                 RDL+NEGIF ++ +VLQ  DKK
Sbjct: 316 RLRSPSTSMESKKNLVYFLHEFCSLSKSLQVVQQLRLFRDLINEGIFHVIEEVLQIPDKK 375

Query: 362 LVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEILRNLL 421
           LVLTGTDILILFL QDP+LLR++VVR EG PL GLLVKGM+ DFGD MHCQFLEI+R LL
Sbjct: 376 LVLTGTDILILFLTQDPNLLRSYVVRTEGNPLLGLLVKGMMEDFGDKMHCQFLEIIRTLL 435

Query: 422 DTYALS-GVQRDTIIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGSLGDKNGVKP 481
           D  ALS G QR  I++IFYEKHL +L+D+ITASCP       +K   A      +   KP
Sbjct: 436 DANALSGGAQRANIMDIFYEKHLPELVDVITASCP-------EKSSNASEGAARRIFTKP 495

Query: 482 EILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVRFIRTVLSRH 541
           E+L NICELLCFC++Q   R K +FL NNV +KVL LT+RKEKYLVVAA+RF+RT+LS H
Sbjct: 496 EVLLNICELLCFCIMQDASRTKCSFLQNNVTEKVLHLTRRKEKYLVVAAIRFVRTLLSVH 555

Query: 542 DEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLVRYIIDSFWN 601
           D+++ NY VKN  +KPIID F+ANG+RYNLLNSAVL+LLE IRK N  LL++YI+D+FW+
Sbjct: 556 DDYVQNYVVKNNLLKPIIDVFIANGTRYNLLNSAVLDLLEHIRKGNATLLLKYIVDTFWD 615

Query: 602 QLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKRNDERALEKEEEDYFNEDSDEE 661
           QL+ F+ L SIQA KVKYEQCLE+   K TS+  DPR+R DERALEKEEEDYFNEDSDEE
Sbjct: 616 QLAPFQCLTSIQAFKVKYEQCLESAGPKSTSDAVDPRRRVDERALEKEEEDYFNEDSDEE 675

Query: 662 DTASAS----VAPKQNVECQRAFP---NGVTTNYPPVSTFYLFSFRSGGLVDYDDDEDDE 721
           D+ASAS      P  N++ ++  P   NGV  +  P S+    S RSGGLVDY+DDEDDE
Sbjct: 676 DSASASNTQKEKPASNIQKEQPKPHLSNGVAAS--PTSS----SPRSGGLVDYEDDEDDE 735

Query: 722 DYRPPHRKQPDTSDEDNEVMEALRQKRKSA-PQEEHEMVKKQRLIKKNKPKDGVFASLCT 768
           DY+PP RKQP+ S++  E  E LR KRKSA  + E E  KK RL K +K ++ VFA LC+
Sbjct: 736 DYKPPPRKQPEASED--EEGELLRLKRKSALVEREQEPSKKPRLGKSSK-RENVFAVLCS 795

BLAST of Lsi05G015840 vs. TAIR 10
Match: AT3G06670.2 (binding )

HSP 1 Score: 815.8 bits (2106), Expect = 2.9e-236
Identity = 493/905 (54.48%), Postives = 596/905 (65.86%), Query Frame = 0

Query: 2   RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
           RVKVY LN+DGKWDD+GTGHV++D+VERSEEL L VIDEEDNETLL+H I+P+DIYRKQE
Sbjct: 16  RVKVYHLNEDGKWDDRGTGHVSIDFVERSEELSLCVIDEEDNETLLVHPINPEDIYRKQE 75

Query: 62  D---------------------------------------------EAFHSVNSELRELP 121
           D                                             E FHS+NSELRELP
Sbjct: 76  DTIISWRDPERSTELALSFQETAGCSYVWDQICTMQRNLHFSSLNSETFHSLNSELRELP 135

Query: 122 AVELSTLPLILKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFK 181
           AVEL+TLPLILK V ESGI DQMRLTEL+L D +FFR LM +F++CEDLEN DGLHMIF 
Sbjct: 136 AVELTTLPLILKIVTESGITDQMRLTELILKDHDFFRNLMGVFKICEDLENVDGLHMIFN 195

Query: 182 IVRGI--------------------------DDPEVPHTQQFRNFLKEHVVFKE------ 241
           IV+GI                           DP VPH+Q  RNFLKEHVVFKE      
Sbjct: 196 IVKGIILLNSSQILEKIFGDELIMEIIGCLEYDPGVPHSQHHRNFLKEHVVFKERQSHVF 255

Query: 242 ---------------AIPIKDPPVLSKIHQTYRVGYLKDVVLARMLDDSMVANLNSIIHA 301
                          AIPIKDP VLSKIHQTYR+GYLKDVVLAR+LDD++VANLNS+IHA
Sbjct: 256 FVRKEHAHYGCFGISAIPIKDPLVLSKIHQTYRIGYLKDVVLARVLDDAIVANLNSVIHA 315

Query: 302 NNAAVVTSLKDDSTFIQELMA--------------------------------------R 361
           NNA VV+ LKDDSTFIQEL A                                      R
Sbjct: 316 NNAIVVSLLKDDSTFIQELFARLRSPSTSMESKKNLVYFLHEFCSLSKSLQVVQQLRLFR 375

Query: 362 DLMNEGIFDIVTDVLQSQDKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKG 421
           DL+NEGIF ++ +VLQ  DKKLVLTGTDILILFL QDP+LLR++VVR EG PL GLLVKG
Sbjct: 376 DLINEGIFHVIEEVLQIPDKKLVLTGTDILILFLTQDPNLLRSYVVRTEGNPLLGLLVKG 435

Query: 422 MVTDFGDNMHCQFLEILRNLLDTYALS-GVQRDTIIEIFYEKHLGQLIDIITASCPTEGF 481
           M+ DFGD MHCQFLEI+R LLD  ALS G QR  I++IFYEKHL +L+D+ITASCP    
Sbjct: 436 MMEDFGDKMHCQFLEIIRTLLDANALSGGAQRANIMDIFYEKHLPELVDVITASCP---- 495

Query: 482 QSAKKIQGAGGSLGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQ 541
              +K   A      +   KPE+L NICELLCFC++Q   R K +FL NNV +KVL LT+
Sbjct: 496 ---EKSSNASEGAARRIFTKPEVLLNICELLCFCIMQDASRTKCSFLQNNVTEKVLHLTR 555

Query: 542 RKEKYLVVAAVRFIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELL 601
           RKEKYLVVAA+RF+RT+LS HD+++ NY VKN  +KPIID F+ANG+RYNLLNSAVL+LL
Sbjct: 556 RKEKYLVVAAIRFVRTLLSVHDDYVQNYVVKNNLLKPIIDVFIANGTRYNLLNSAVLDLL 615

Query: 602 EFIRKENLKLLVRYIIDSFWNQLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRKR 661
           E IRK N  LL++YI+D+FW+QL+ F+ L SIQA KVKYEQCLE+   K TS+  DPR+R
Sbjct: 616 EHIRKGNATLLLKYIVDTFWDQLAPFQCLTSIQAFKVKYEQCLESAGPKSTSDAVDPRRR 675

Query: 662 NDERALEKEEEDYFNEDSDEEDTASAS----VAPKQNVECQRAFP---NGVTTNYPPVST 721
            DERALEKEEEDYFNEDSDEED+ASAS      P  N++ ++  P   NGV  +  P S+
Sbjct: 676 VDERALEKEEEDYFNEDSDEEDSASASNTQKEKPASNIQKEQPKPHLSNGVAAS--PTSS 735

Query: 722 FYLFSFRSGGLVDYDDDEDDEDYRPPHRKQPDTSDEDNEVMEALRQKRKSA-PQEEHEMV 768
               S RSGGLVDY+DDEDDEDY+PP RKQP+ S++  E  E LR KRKSA  + E E  
Sbjct: 736 ----SPRSGGLVDYEDDEDDEDYKPPPRKQPEASED--EEGELLRLKRKSALVEREQEPS 795

BLAST of Lsi05G015840 vs. TAIR 10
Match: AT5G49390.1 (LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 11 growth stages; BEST Arabidopsis thaliana protein match is: binding (TAIR:AT3G06670.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 240.4 bits (612), Expect = 4.9e-63
Identity = 156/328 (47.56%), Postives = 206/328 (62.80%), Query Frame = 0

Query: 248 SQDKKLVLTGTDILILFLNQDPSLLRTHVVRQEGFPLFGLLVKGMVTDFGDNMHCQFLEI 307
           S DKKLVL G  IL + L QD   L ++VVR E + L GLLVKGM+ DFGD M   F++I
Sbjct: 2   SSDKKLVLMGAKILSVLLAQDSIRLCSYVVRPETY-LLGLLVKGMMEDFGDEMESLFVDI 61

Query: 308 LRNLLDTYALSGVQRDT-----IIEIFYEKHLGQLIDIITASCPTEGFQSAKKIQGAGGS 367
           ++N+L         + T     I +IF EKHL +L+D I ASCP     ++   +GA   
Sbjct: 62  IQNVLGCGGAQVSDKKTNMQRIIWDIFCEKHLPELVDFIMASCPERPGDTS---EGAFVR 121

Query: 368 LGDKNGVKPEILSNICELLCFCVLQHPYRIKSNFLLNNVIDKVLLLTQRKEKYLVVAAVR 427
           +G   G KP IL  ICELLC CV   P R  +NFL N+VI+KVLLLT+RKEK LV AAVR
Sbjct: 122 VGSHRGTKPGILLRICELLCSCVQLDPSR--TNFLHNSVIEKVLLLTRRKEKTLVAAAVR 181

Query: 428 FIRTVLSRHDEHLINYFVKNGTIKPIIDAFVANGSRYNLLNSAVLELLEFIRKENLKLLV 487
           F+RT+LS H++++ +Y V+N T+KPII+ FVAN  R NL+ +AVLELLE IRKE   +LV
Sbjct: 182 FVRTLLSVHNDNVQSYIVENNTLKPIIEVFVANRHRDNLMTAAVLELLEHIRKE-YAVLV 241

Query: 488 RYIIDSFWNQLSKFENLPSIQALKVKYEQCLENCEMKGTSNTSDPRK--RNDERALEK-- 547
           +Y+ D+FW+QL+ FENL SI+ LK KYE        KG  +T+D     R D RAL++  
Sbjct: 242 KYVGDTFWDQLAPFENLRSIKDLKTKYE--------KGPKSTTDDESDMRQDGRALDEKK 301

Query: 548 ---------EEEDYFNEDSDEEDTASAS 558
                    EE D  N ++    +AS++
Sbjct: 302 PASASSTQMEEADPHNSNATAASSASST 314

BLAST of Lsi05G015840 vs. TAIR 10
Match: AT5G49380.1 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF625 (InterPro:IPR006887); BEST Arabidopsis thaliana protein match is: binding (TAIR:AT3G06670.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 133.3 bits (334), Expect = 8.5e-31
Identity = 79/171 (46.20%), Postives = 107/171 (62.57%), Query Frame = 0

Query: 87  LKTVVESGIGDQMRLTELLLNDKEFFRKLMNLFRVCEDLENTDGLHMIFKIVRGI----- 146
           L+ V + G  DQMRLTEL+L +  FF+KL+++F  CE+ ++ DGLHM+F IV+ I     
Sbjct: 8   LQVVADYGNTDQMRLTELMLKNGAFFQKLIDVFDDCENRKDIDGLHMMFNIVKEIISVNN 67

Query: 147 ---------------------DDPEVPHTQQFRNFLKEHVVFKEAIPIKDPPVLSKIHQT 206
                                 DP+VP ++  R  L+++VVF E IPIK+P VLSKIHQT
Sbjct: 68  YQILEIILGDQLFMKIFGCLEYDPDVPQSKDHRTSLRKNVVFVEDIPIKNPLVLSKIHQT 127

Query: 207 YRVGYLKDVVLARMLDDSMVANLNSIIHANNAAVVTSLKDDSTFIQELMAR 232
           YR+ +LKDVVL  +LD +  A L+S+I+AN A V+T LKDD   IQE  AR
Sbjct: 128 YRIDFLKDVVLTDVLDVATSAFLDSVINANKATVLTLLKDD---IQESFAR 175

BLAST of Lsi05G015840 vs. TAIR 10
Match: AT5G49370.1 (Pleckstrin homology (PH) domain superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 1.0e-20
Identity = 43/61 (70.49%), Postives = 55/61 (90.16%), Query Frame = 0

Query: 2  RVKVYRLNDDGKWDDQGTGHVTVDYVERSEELGLFVIDEEDNETLLLHRISPDDIYRKQE 61
          RVKVYRLN+DG+WDD+GTGH+T+DY+ERSE   L+VIDE+DN TLL HRIS D+IY++Q+
Sbjct: 12 RVKVYRLNEDGQWDDKGTGHITMDYMERSEVFNLYVIDEDDNATLLAHRISIDNIYKQQD 71

Query: 62 D 63
          D
Sbjct: 72 D 72