Lsi05G014520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G014520
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAquaporin NIP1.1, putative
Locationchr05: 22377389 .. 22379581 (-)
RNA-Seq ExpressionLsi05G014520
SyntenyLsi05G014520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCAACAACCACTGTCCCAACTCCAACAAATTATGACTTCAAATAATAATAATAATAATAATACAATCCCCGTCGTCGTCTCCGCCACCTTCCCACAACCATCAACGATGGACCATAATCTAGCTCGCCCGGTAACGCTCCTAATATTCCGATTACAATAACAATGTGGCTGCGACCGCCCAGTGGTTCTAATATGTTTTTGTTTGTTTTGGTGTAGGTTTTGGGAGAGATGGTGGGGAGTTTTTTGTTGATGATTTGTGTGAGCGGGGTGACGGTGACCGGGCAGCTGCCGGGTGGTCAGATGGGTCTATTGGACTATGCCGTCGCCGCCGGTTTGACGGTCGGCGTTTTGACGTTCTGCTTTGCTCCCATTTCCGGTGCTCATTTTAATCCTGCTATCACTCTTGCCTCTGCCATTTTTGGTCACTTTCCATGGTCTAGGGTACTAATACTACTTTCTTCTTTTTACATTTTATTTCAAAAAATAAATGTGAACAACAGGATGTATAATGTAACTACATCCTGTTTGTTTTGTTTGGAAAATTAGGTAATGCCATATGCAGTGGCTCAAACAACAGGTTGTATAATGGCAACATATGCAGCAATATTTGTTTACGGCATAAAACCTCAACAATTGACTACTCGACCATTTTATTCTCCAATTTCTGCCTTCTTCCTTGAGTTTCTTGCAACTTTCATTCTCATGTTTCTCCTTTCTTCTTTAACCTATCAACCCCAATCAGTAAGTCTTAAATATTTTATATCAGGCCTCCACTTAATTTTATGTTTTTATCTTATATATAAGTTAGTATCAAACATTTAAAAAATAAATTCAAAAAACTTACCATTTGGGTTTTATTGAGCCGAAGTTAATATGATGCAGATTGGTCAATTATCGGGCTTTGTCATTGGAATGGCCGTTGGGCTTGCTGTGTTTATCGCCGGGTAGGTGCTGCCCCTTTTTCTTCTCATTATTATCAAAATAATTTTAGCCACAATCTTTTTGATACAACCGATCTAATTGCTATGTGACAATTTTTTTTTAAAAAAAATTATATATTTTTTTATTGAAAGATATGCATGGAGTTACATCTAAGTATTACTCTGACTAGAGGATGAGAAAATATTTTTTTGGTTCTTATCTTTTGAGTATAATTTCTATTTGGTACCAAAGTTTCAAACGGTTATACTTTTACTTTCAATTTAGGTTTAATTTTTATTTGGCTCTTAGATTGAGTTTTTACTAATCTCACTTTGTTATTTGACATTAACTTTTAATTAATTAATTTAAAAGAATTATGATAATAAGTATTAAATTAATTTTAACAATGATGAAAAATTAGTGGAAACTAATTTAATTCTTTTAAATTAATTAACAGACATTAACAACAAAGACTAAAAGTGAAACTTAAAAAAGAAAATTAAAGTTAAAAGAGTAAATCTTGAAGTTATATGGACTAAATCGAAACAAAACTCAAACGTAAAAATGTAAAATTTTAGAAGTTAAGGACTAAATAAAATTTAAACTCAGAATTCAAATGTAGAAGTGTAACATTTTGAAAATTTATAAACCAAATAAGACTTAAAAGACAAAACAAAAAAAGAAATTATAGCACAACATGAAAATGGGAGGTATGGAACTTCTCCTACCAAGCTACTTTGTTAATGAATTTTTATACGCGTTTGGATTGAATTTTTATATAAATACTTATTTAAACACTTAGAAAATTAATCCAAATTGACTCTAAGATAGTCGAAACTTGATTGAAAAGTTATAACTCATAAAAAAAAAATGAAATTAAGACTTTTAAAATATAAAATTATATAATTATATACTTTTTATTTTATTATTAAAAGTGTCTGATGAGTTTTGGGCCACAGGCCCATTTCAGGTGCATCAATGAACCCAGCAAGATCTTTAGGGCCAGCAATTGTTTCATGGGCTTTCAACGACATATGGATCTATATTACAGCTCCGATCATCGGAGCCGTTACCGGTACCTTCTTCAGCGGCGTCCTCCGCCTTCATCCTCCGCCGCTCCATTCATCCTCCTCCCCTTCCGACAGTAGACTTTTTCATCATTCCTCCTCAGCCAATGCATATCTAATTACTTAGCTAATCATCTCCTTCATCATCCCTATATTTGTAGTTGCGTCATATATTCAAATGGAATAACAATTTGGTTTTAAAA

mRNA sequence

CTTCAACAACCACTGTCCCAACTCCAACAAATTATGACTTCAAATAATAATAATAATAATAATACAATCCCCGTCGTCGTCTCCGCCACCTTCCCACAACCATCAACGATGGACCATAATCTAGCTCGCCCGGTTTTGGGAGAGATGGTGGGGAGTTTTTTGTTGATGATTTGTGTGAGCGGGGTGACGGTGACCGGGCAGCTGCCGGGTGGTCAGATGGGTCTATTGGACTATGCCGTCGCCGCCGGTTTGACGGTCGGCGTTTTGACGTTCTGCTTTGCTCCCATTTCCGGTGCTCATTTTAATCCTGCTATCACTCTTGCCTCTGCCATTTTTGGTCACTTTCCATGGTCTAGGGTAATGCCATATGCAGTGGCTCAAACAACAGGTTGTATAATGGCAACATATGCAGCAATATTTGTTTACGGCATAAAACCTCAACAATTGACTACTCGACCATTTTATTCTCCAATTTCTGCCTTCTTCCTTGAGTTTCTTGCAACTTTCATTCTCATGTTTCTCCTTTCTTCTTTAACCTATCAACCCCAATCAATTGGTCAATTATCGGGCTTTGTCATTGGAATGGCCGTTGGGCTTGCTGTGTTTATCGCCGGGCCCATTTCAGGTGCATCAATGAACCCAGCAAGATCTTTAGGGCCAGCAATTGTTTCATGGGCTTTCAACGACATATGGATCTATATTACAGCTCCGATCATCGGAGCCGTTACCGGTACCTTCTTCAGCGGCGTCCTCCGCCTTCATCCTCCGCCGCTCCATTCATCCTCCTCCCCTTCCGACAGTAGACTTTTTCATCATTCCTCCTCAGCCAATGCATATCTAATTACTTAGCTAATCATCTCCTTCATCATCCCTATATTTGTAGTTGCGTCATATATTCAAATGGAATAACAATTTGGTTTTAAAA

Coding sequence (CDS)

ATGACTTCAAATAATAATAATAATAATAATACAATCCCCGTCGTCGTCTCCGCCACCTTCCCACAACCATCAACGATGGACCATAATCTAGCTCGCCCGGTTTTGGGAGAGATGGTGGGGAGTTTTTTGTTGATGATTTGTGTGAGCGGGGTGACGGTGACCGGGCAGCTGCCGGGTGGTCAGATGGGTCTATTGGACTATGCCGTCGCCGCCGGTTTGACGGTCGGCGTTTTGACGTTCTGCTTTGCTCCCATTTCCGGTGCTCATTTTAATCCTGCTATCACTCTTGCCTCTGCCATTTTTGGTCACTTTCCATGGTCTAGGGTAATGCCATATGCAGTGGCTCAAACAACAGGTTGTATAATGGCAACATATGCAGCAATATTTGTTTACGGCATAAAACCTCAACAATTGACTACTCGACCATTTTATTCTCCAATTTCTGCCTTCTTCCTTGAGTTTCTTGCAACTTTCATTCTCATGTTTCTCCTTTCTTCTTTAACCTATCAACCCCAATCAATTGGTCAATTATCGGGCTTTGTCATTGGAATGGCCGTTGGGCTTGCTGTGTTTATCGCCGGGCCCATTTCAGGTGCATCAATGAACCCAGCAAGATCTTTAGGGCCAGCAATTGTTTCATGGGCTTTCAACGACATATGGATCTATATTACAGCTCCGATCATCGGAGCCGTTACCGGTACCTTCTTCAGCGGCGTCCTCCGCCTTCATCCTCCGCCGCTCCATTCATCCTCCTCCCCTTCCGACAGTAGACTTTTTCATCATTCCTCCTCAGCCAATGCATATCTAATTACTTAG

Protein sequence

MTSNNNNNNNTIPVVVSATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLTTRPFYSPISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPLHSSSSPSDSRLFHHSSSANAYLIT
Homology
BLAST of Lsi05G014520 vs. ExPASy Swiss-Prot
Match: Q8LAI1 (Probable aquaporin NIP7-1 OS=Arabidopsis thaliana OX=3702 GN=NIP7-1 PE=2 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 1.1e-57
Identity = 122/237 (51.48%), Postives = 158/237 (66.67%), Query Frame = 0

Query: 23  PSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCF 82
           P  +D N  R V+ E+VG+F+LM  V GV  + QL GG +GLL+YAV AGL+V V+ +  
Sbjct: 37  PYDIDLNPLRIVMAELVGTFILMFSVCGVISSTQLSGGHVGLLEYAVTAGLSVVVVVYSI 96

Query: 83  APISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLTTRP 142
             ISGAH NP+IT+A A+FG FPWS+V  Y  AQT G   AT   + VYG+    + T+P
Sbjct: 97  GHISGAHLNPSITIAFAVFGGFPWSQVPLYITAQTLGATAATLVGVSVYGVNADIMATKP 156

Query: 143 FYSPISAFFLEFLATFILMFLLSSLTYQP-QSIGQLSGFVIGMAVGLAVFIAGPISGASM 202
             S +SAFF+E +AT I++FL S+L   P Q++G L+GFVIG  + L V I GPISG SM
Sbjct: 157 ALSCVSAFFVELIATSIVVFLASALHCGPHQNLGNLTGFVIGTVISLGVLITGPISGGSM 216

Query: 203 NPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPLHSSSSPSDSRL 259
           NPARSLGPA+V+W F D+WIY+TAP+IGA+ G      + L   P  S  SPS S L
Sbjct: 217 NPARSLGPAVVAWDFEDLWIYMTAPVIGAIIGVLTYRSISLKTRPCPSPVSPSVSSL 273

BLAST of Lsi05G014520 vs. ExPASy Swiss-Prot
Match: Q9FIZ9 (Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.2e-39
Identity = 86/219 (39.27%), Postives = 131/219 (59.82%), Query Frame = 0

Query: 30  LARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAH 89
           L + ++ EM+G++ ++    GV V   L GG +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 90  FNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQ-LTTRPFYSPIS 149
           FNPA+T+  AIF  FPW +V  Y  AQ  G ++A+     ++ + P+    T P  SP  
Sbjct: 101 FNPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPAR 160

Query: 150 AFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLG 209
           A   E + +F+LMF++S +    +++G+L+G  +GM + + VF+AGPISGASMNPARSLG
Sbjct: 161 ALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLG 220

Query: 210 PAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
           PA+V   +  IW+YI  P++G ++G F   ++R    PL
Sbjct: 221 PALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPL 259

BLAST of Lsi05G014520 vs. ExPASy Swiss-Prot
Match: Q8W036 (Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-37
Identity = 87/219 (39.73%), Postives = 126/219 (57.53%), Query Frame = 0

Query: 30  LARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAH 89
           L + ++ EM+G++ ++    GV V   L GG +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 90  FNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQ-LTTRPFYSPIS 149
           FNPA+T+  A+F  FPW +V  Y  AQ TG ++A+     ++ + P+    T P  S   
Sbjct: 101 FNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQ 160

Query: 150 AFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLG 209
           A   E + +F+LMF++S +    ++ G+L+G  +GM + L VF+AGPISGASMNPARSLG
Sbjct: 161 ALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLG 220

Query: 210 PAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
           PAIV   +  IW+YI  P +G   G F    +R    PL
Sbjct: 221 PAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPL 259

BLAST of Lsi05G014520 vs. ExPASy Swiss-Prot
Match: Q40746 (Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-1 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.1e-37
Identity = 86/215 (40.00%), Postives = 123/215 (57.21%), Query Frame = 0

Query: 34  VLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPA 93
           ++ E+ G++ L+    G     Q   GQ+     A+  GL V V+ +    ISGAHFNPA
Sbjct: 50  IIAEIFGTYFLIFAGCGAVTINQSKNGQITFPGVAIVWGLAVMVMVYAVGHISGAHFNPA 109

Query: 94  ITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLT-TRPFYSPISAFFL 153
           +TLA A    FPW +V  YA AQ  G  +A      ++G + +    T P  S + +  L
Sbjct: 110 VTLAFATCRRFPWRQVPAYAAAQMLGATLAAGTLRLMFGGRHEHFPGTLPAGSDVQSLVL 169

Query: 154 EFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLGPAIV 213
           EF+ TF LMF++S +    ++IG+L+G  +G  + L V IAGPISGASMNPARSLGPA++
Sbjct: 170 EFIITFYLMFVISGVATDNRAIGELAGLAVGATILLNVLIAGPISGASMNPARSLGPAMI 229

Query: 214 SWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
              +  IW+YI  P+ GAV G +   ++R    PL
Sbjct: 230 GGEYRSIWVYIVGPVAGAVAGAWAYNIIRFTNKPL 264

BLAST of Lsi05G014520 vs. ExPASy Swiss-Prot
Match: Q9ATN4 (Aquaporin NIP1-1 OS=Zea mays OX=4577 GN=NIP1-1 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.7e-37
Identity = 82/215 (38.14%), Postives = 125/215 (58.14%), Query Frame = 0

Query: 34  VLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPA 93
           ++ E+ G++ LM    G         GQ+     A+  GL V V+ +    ISGAHFNPA
Sbjct: 46  IIAEIFGTYFLMFAGCGAVTINASKNGQITFPGVAIVWGLAVMVMVYAVGHISGAHFNPA 105

Query: 94  ITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLT-TRPFYSPISAFFL 153
           +TLA A  G FPW ++  Y +AQ  G  +A+     ++G + +    T P  S + +  +
Sbjct: 106 VTLAFATSGRFPWRQLPAYVLAQMLGATLASGTLRLMFGGRHEHFPGTLPTGSEVQSLVI 165

Query: 154 EFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLGPAIV 213
           E + TF LMF++S +    ++IG+L+G  +G  + L V IAGP+SGASMNPARS+GPA+V
Sbjct: 166 EIITTFYLMFVISGVATDNRAIGELAGLAVGATILLNVLIAGPVSGASMNPARSVGPALV 225

Query: 214 SWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
           S  +  IW+Y+  P++GAV G +   ++R    PL
Sbjct: 226 SGEYTSIWVYVVGPVVGAVAGAWAYNLIRFTNKPL 260

BLAST of Lsi05G014520 vs. ExPASy TrEMBL
Match: A0A1S3AX29 (probable aquaporin NIP7-1 OS=Cucumis melo OX=3656 GN=LOC103483738 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 4.1e-113
Identity = 218/270 (80.74%), Postives = 235/270 (87.04%), Query Frame = 0

Query: 5   NNNNNNTIPVVVSATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGL 64
           +NNNNNTIPV VSATFP+P+TMD NL RPVLGEMVGSFLL++CVSGVT TGQL G QMG+
Sbjct: 3   SNNNNNTIPVTVSATFPRPTTMDDNLVRPVLGEMVGSFLLIVCVSGVTATGQLTGSQMGV 62

Query: 65  LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMAT 124
           LDYA+AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVM Y VAQTTGC+MAT
Sbjct: 63  LDYAIAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMAYVVAQTTGCVMAT 122

Query: 125 YAAIFVYGIKPQQLTTRPFY---SPISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFV 184
           YAA+FV+GIKPQQL TRP +   SP SAFFLE L TFILMFLLSSL+YQ QS+ Q SGFV
Sbjct: 123 YAAMFVFGIKPQQLITRPLHNYSSPFSAFFLELLLTFILMFLLSSLSYQSQSVRQFSGFV 182

Query: 185 IGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLR 244
           IGMA+ LAVFIAGPISGASMNPARSLGPAIVSWAF+DIWIYITAP IGA+TG F S  LR
Sbjct: 183 IGMAIALAVFIAGPISGASMNPARSLGPAIVSWAFDDIWIYITAPCIGAITGAFISDFLR 242

Query: 245 LHPPPLHSSSSPSDSRLFHHSSSANAYLIT 272
           L PPPL     PSD + F  SSSANAYLIT
Sbjct: 243 LCPPPL----QPSDGKHFDLSSSANAYLIT 268

BLAST of Lsi05G014520 vs. ExPASy TrEMBL
Match: A0A0A0LAF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149960 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 2.1e-112
Identity = 217/270 (80.37%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 5   NNNNNNTIPVVVSATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGL 64
           +NNNNNTIPV VSATFPQP+TMDHNL RPVLGEMVGSFLL++CVSGVT TGQL G QMG+
Sbjct: 3   SNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGI 62

Query: 65  LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMAT 124
           LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWSRVM Y VAQTTGC+MAT
Sbjct: 63  LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMAT 122

Query: 125 YAAIFVYGIKPQQLTTRPFY---SPISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFV 184
           YAA+FV+GIKPQQL TRP Y   SP SAFFLE L TFILMFLLSSL++Q Q + Q SGFV
Sbjct: 123 YAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVRQFSGFV 182

Query: 185 IGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLR 244
           IGMA+ LAVFIAGPISGASMNPARSLGPAIVSWAF+DIWIYITAP IGA+TG F S  LR
Sbjct: 183 IGMAIALAVFIAGPISGASMNPARSLGPAIVSWAFDDIWIYITAPAIGAITGAFISDFLR 242

Query: 245 LHPPPLHSSSSPSDSRLFHHSSSANAYLIT 272
           L PPP      PS+++ F  SSSANAYLIT
Sbjct: 243 LSPPP----PQPSNAKYFDVSSSANAYLIT 268

BLAST of Lsi05G014520 vs. ExPASy TrEMBL
Match: A0A6J1IR00 (probable aquaporin NIP7-1 OS=Cucurbita maxima OX=3661 GN=LOC111479196 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.2e-107
Identity = 210/272 (77.21%), Postives = 229/272 (84.19%), Query Frame = 0

Query: 6   NNNNNTIPVVVSAT-----FPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGG 65
           +NN N IP  VS        PQP+ MDHN ARPVLGEM+G+FLLMICVSGV  TGQ+ GG
Sbjct: 3   SNNTNPIPTAVSGNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQMMGG 62

Query: 66  QMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGC 125
           Q+GLLDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWS VMPY VAQTTGC
Sbjct: 63  QVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQTTGC 122

Query: 126 IMATYAAIFVYGIKPQQLTTRPFYSPISAFFLEFLATFILMFLLSSLTYQ--PQSIGQLS 185
           +MATYA +FVY IKPQQLTTRPF   +SAFF+E LATFILMFL+SSL +Q  P+  GQLS
Sbjct: 123 VMATYAGMFVYDIKPQQLTTRPFRGSMSAFFVELLATFILMFLISSLPHQSRPRPDGQLS 182

Query: 186 GFVIGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSG 245
           GFVIGMA+GLAVFIAGPISG SMNPARSLGPA+VSWAF+DIWIYITAP+IGAVTG FFSG
Sbjct: 183 GFVIGMAIGLAVFIAGPISGGSMNPARSLGPAMVSWAFDDIWIYITAPVIGAVTGAFFSG 242

Query: 246 VLRLHPPPLHSSSSPSDSRLFHHSSSANAYLI 271
           VLRLHPPP+HS    SD   F  SSSAN +LI
Sbjct: 243 VLRLHPPPIHS----SDGGNFRRSSSANLHLI 270

BLAST of Lsi05G014520 vs. ExPASy TrEMBL
Match: A0A6J1GVJ7 (probable aquaporin NIP7-1 OS=Cucurbita moschata OX=3662 GN=LOC111457868 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.6e-107
Identity = 210/272 (77.21%), Postives = 229/272 (84.19%), Query Frame = 0

Query: 6   NNNNNTIPVVVS-----ATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGG 65
           +NN N IP  VS        PQP+ MDHN ARPVLGEM+G+FLLMICVSGV  TGQ+ GG
Sbjct: 3   SNNTNPIPTAVSDNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQMMGG 62

Query: 66  QMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGC 125
           Q+GLLDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWS VMPY VAQTTGC
Sbjct: 63  QVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQTTGC 122

Query: 126 IMATYAAIFVYGIKPQQLTTRPFYSPISAFFLEFLATFILMFLLSSLTYQ--PQSIGQLS 185
           +MATYA +FVY IKPQQLTTRPF   +SAFF+E LATFILMFL+SSL +Q  P+  GQLS
Sbjct: 123 VMATYAGMFVYDIKPQQLTTRPFRGSMSAFFVELLATFILMFLISSLPHQSRPRPGGQLS 182

Query: 186 GFVIGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSG 245
           GFVIGMA+GLAVFIAGPISG SMNPARSLGPA+VSWAF+DIWIYITAP+IGAVTG FFSG
Sbjct: 183 GFVIGMAIGLAVFIAGPISGGSMNPARSLGPAMVSWAFDDIWIYITAPVIGAVTGAFFSG 242

Query: 246 VLRLHPPPLHSSSSPSDSRLFHHSSSANAYLI 271
           VLRLHPPP+HS    SD   F  SSSAN +LI
Sbjct: 243 VLRLHPPPIHS----SDGGNFRRSSSANLHLI 270

BLAST of Lsi05G014520 vs. ExPASy TrEMBL
Match: A0A6J1C8T1 (probable aquaporin NIP7-1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009435 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.1e-92
Identity = 187/246 (76.02%), Postives = 202/246 (82.11%), Query Frame = 0

Query: 26  MDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPI 85
           MD N ARPVL EMVGSFLLM+CV G T +     G MGL DYAVAAGLTVGVLTF F PI
Sbjct: 1   MDPNPARPVLAEMVGSFLLMVCVCGTTAS----AGLMGLFDYAVAAGLTVGVLTFSFRPI 60

Query: 86  SGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLTTRPFYS 145
           SGAHFNPAITLASAIF HFPWS VMPYAVAQTTGC+MATYA +F+Y IKPQQLTTRPF  
Sbjct: 61  SGAHFNPAITLASAIFSHFPWSLVMPYAVAQTTGCVMATYAGMFIYNIKPQQLTTRPFQP 120

Query: 146 PISAFFLEFLATFILMFLLSSLT-YQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPA 205
            ISAF +E LA FILMFL+SSL  +Q QS+GQ SGFVIGMA+GLAV I GPISG SMNPA
Sbjct: 121 TISAFLVELLAAFILMFLVSSLAHHQHQSVGQFSGFVIGMAIGLAVLITGPISGGSMNPA 180

Query: 206 RSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPLHSSSSPSDSRLFHHSSS 265
           RSLGPAIVSWAF+DIWIYITAP+IGAV+G F  GVLRL PP  +S SS S +RLF  SSS
Sbjct: 181 RSLGPAIVSWAFDDIWIYITAPVIGAVSGAFLYGVLRLRPPRANSPSSASTTRLFPRSSS 240

Query: 266 ANAYLI 271
           ANA LI
Sbjct: 241 ANACLI 242

BLAST of Lsi05G014520 vs. NCBI nr
Match: XP_008438714.1 (PREDICTED: probable aquaporin NIP7-1 [Cucumis melo])

HSP 1 Score: 417.5 bits (1072), Expect = 8.5e-113
Identity = 218/270 (80.74%), Postives = 235/270 (87.04%), Query Frame = 0

Query: 5   NNNNNNTIPVVVSATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGL 64
           +NNNNNTIPV VSATFP+P+TMD NL RPVLGEMVGSFLL++CVSGVT TGQL G QMG+
Sbjct: 3   SNNNNNTIPVTVSATFPRPTTMDDNLVRPVLGEMVGSFLLIVCVSGVTATGQLTGSQMGV 62

Query: 65  LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMAT 124
           LDYA+AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVM Y VAQTTGC+MAT
Sbjct: 63  LDYAIAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMAYVVAQTTGCVMAT 122

Query: 125 YAAIFVYGIKPQQLTTRPFY---SPISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFV 184
           YAA+FV+GIKPQQL TRP +   SP SAFFLE L TFILMFLLSSL+YQ QS+ Q SGFV
Sbjct: 123 YAAMFVFGIKPQQLITRPLHNYSSPFSAFFLELLLTFILMFLLSSLSYQSQSVRQFSGFV 182

Query: 185 IGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLR 244
           IGMA+ LAVFIAGPISGASMNPARSLGPAIVSWAF+DIWIYITAP IGA+TG F S  LR
Sbjct: 183 IGMAIALAVFIAGPISGASMNPARSLGPAIVSWAFDDIWIYITAPCIGAITGAFISDFLR 242

Query: 245 LHPPPLHSSSSPSDSRLFHHSSSANAYLIT 272
           L PPPL     PSD + F  SSSANAYLIT
Sbjct: 243 LCPPPL----QPSDGKHFDLSSSANAYLIT 268

BLAST of Lsi05G014520 vs. NCBI nr
Match: XP_004134455.2 (probable aquaporin NIP7-1 [Cucumis sativus] >KGN57016.1 hypothetical protein Csa_011741 [Cucumis sativus])

HSP 1 Score: 415.2 bits (1066), Expect = 4.2e-112
Identity = 217/270 (80.37%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 5   NNNNNNTIPVVVSATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGL 64
           +NNNNNTIPV VSATFPQP+TMDHNL RPVLGEMVGSFLL++CVSGVT TGQL G QMG+
Sbjct: 3   SNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGI 62

Query: 65  LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMAT 124
           LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWSRVM Y VAQTTGC+MAT
Sbjct: 63  LDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMAT 122

Query: 125 YAAIFVYGIKPQQLTTRPFY---SPISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFV 184
           YAA+FV+GIKPQQL TRP Y   SP SAFFLE L TFILMFLLSSL++Q Q + Q SGFV
Sbjct: 123 YAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVRQFSGFV 182

Query: 185 IGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLR 244
           IGMA+ LAVFIAGPISGASMNPARSLGPAIVSWAF+DIWIYITAP IGA+TG F S  LR
Sbjct: 183 IGMAIALAVFIAGPISGASMNPARSLGPAIVSWAFDDIWIYITAPAIGAITGAFISDFLR 242

Query: 245 LHPPPLHSSSSPSDSRLFHHSSSANAYLIT 272
           L PPP      PS+++ F  SSSANAYLIT
Sbjct: 243 LSPPP----PQPSNAKYFDVSSSANAYLIT 268

BLAST of Lsi05G014520 vs. NCBI nr
Match: KAG6582276.1 (putative aquaporin NIP7-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 400.6 bits (1028), Expect = 1.1e-107
Identity = 211/272 (77.57%), Postives = 230/272 (84.56%), Query Frame = 0

Query: 6   NNNNNTIPVVVS-----ATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGG 65
           +NN N IP  VS        PQP+ MDHN ARPVLGEM+G+FLLMICVSGV  TGQ+ GG
Sbjct: 3   SNNTNPIPTAVSDNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQMMGG 62

Query: 66  QMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGC 125
           Q+GLLDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWS VMPY VAQTTGC
Sbjct: 63  QVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQTTGC 122

Query: 126 IMATYAAIFVYGIKPQQLTTRPFYSPISAFFLEFLATFILMFLLSSLTYQ--PQSIGQLS 185
           +MATYA +FVY IKPQQLTTRPF   +SAFF+E LATFILMFL+SSL +Q  P+  GQLS
Sbjct: 123 VMATYAGMFVYDIKPQQLTTRPFRGSMSAFFVELLATFILMFLISSLPHQSRPRPDGQLS 182

Query: 186 GFVIGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSG 245
           GFVIGMA+GLAVFIAGPISG SMNPARSLGPAIVSWAF+DIWIYITAP+IGAVTG FFSG
Sbjct: 183 GFVIGMAIGLAVFIAGPISGGSMNPARSLGPAIVSWAFDDIWIYITAPVIGAVTGAFFSG 242

Query: 246 VLRLHPPPLHSSSSPSDSRLFHHSSSANAYLI 271
           VLRLHPPP+HS    SD   F  SSSAN++LI
Sbjct: 243 VLRLHPPPIHS----SDGGNFRRSSSANSHLI 270

BLAST of Lsi05G014520 vs. NCBI nr
Match: XP_022979516.1 (probable aquaporin NIP7-1 [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 2.4e-107
Identity = 210/272 (77.21%), Postives = 229/272 (84.19%), Query Frame = 0

Query: 6   NNNNNTIPVVVSAT-----FPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGG 65
           +NN N IP  VS        PQP+ MDHN ARPVLGEM+G+FLLMICVSGV  TGQ+ GG
Sbjct: 3   SNNTNPIPTAVSGNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQMMGG 62

Query: 66  QMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGC 125
           Q+GLLDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWS VMPY VAQTTGC
Sbjct: 63  QVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQTTGC 122

Query: 126 IMATYAAIFVYGIKPQQLTTRPFYSPISAFFLEFLATFILMFLLSSLTYQ--PQSIGQLS 185
           +MATYA +FVY IKPQQLTTRPF   +SAFF+E LATFILMFL+SSL +Q  P+  GQLS
Sbjct: 123 VMATYAGMFVYDIKPQQLTTRPFRGSMSAFFVELLATFILMFLISSLPHQSRPRPDGQLS 182

Query: 186 GFVIGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSG 245
           GFVIGMA+GLAVFIAGPISG SMNPARSLGPA+VSWAF+DIWIYITAP+IGAVTG FFSG
Sbjct: 183 GFVIGMAIGLAVFIAGPISGGSMNPARSLGPAMVSWAFDDIWIYITAPVIGAVTGAFFSG 242

Query: 246 VLRLHPPPLHSSSSPSDSRLFHHSSSANAYLI 271
           VLRLHPPP+HS    SD   F  SSSAN +LI
Sbjct: 243 VLRLHPPPIHS----SDGGNFRRSSSANLHLI 270

BLAST of Lsi05G014520 vs. NCBI nr
Match: XP_023528366.1 (probable aquaporin NIP7-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.4 bits (1025), Expect = 2.4e-107
Identity = 211/272 (77.57%), Postives = 229/272 (84.19%), Query Frame = 0

Query: 6   NNNNNTIPVVVS-----ATFPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGG 65
           +NN N IP  VS        PQP+ MDHN ARPVLGEM+G+FLLMICVSGV  TGQ+ GG
Sbjct: 3   SNNTNPIPTAVSDNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQMMGG 62

Query: 66  QMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGC 125
           Q+GLLDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWS VMPY VAQTTGC
Sbjct: 63  QVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQTTGC 122

Query: 126 IMATYAAIFVYGIKPQQLTTRPFYSPISAFFLEFLATFILMFLLSSLTYQ--PQSIGQLS 185
           +MATYA +FVY IKPQQLTTRPF   +SAFF+E LATFILMFL+SSL +Q  P+  GQLS
Sbjct: 123 VMATYAGMFVYDIKPQQLTTRPFRGSMSAFFVELLATFILMFLISSLPHQSRPRPGGQLS 182

Query: 186 GFVIGMAVGLAVFIAGPISGASMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSG 245
           GFVIGMA+GLAVFIAGPISG SMNPARSLGPAIVSWAF+DIWIYITAP+IGAVTG FFSG
Sbjct: 183 GFVIGMAIGLAVFIAGPISGGSMNPARSLGPAIVSWAFDDIWIYITAPVIGAVTGAFFSG 242

Query: 246 VLRLHPPPLHSSSSPSDSRLFHHSSSANAYLI 271
           VLRLHPPP+HS    SD   F  SSSAN +LI
Sbjct: 243 VLRLHPPPIHS----SDGGNFRRSSSANLHLI 270

BLAST of Lsi05G014520 vs. TAIR 10
Match: AT3G06100.1 (NOD26-like intrinsic protein 7;1 )

HSP 1 Score: 224.9 bits (572), Expect = 7.6e-59
Identity = 122/237 (51.48%), Postives = 158/237 (66.67%), Query Frame = 0

Query: 23  PSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCF 82
           P  +D N  R V+ E+VG+F+LM  V GV  + QL GG +GLL+YAV AGL+V V+ +  
Sbjct: 37  PYDIDLNPLRIVMAELVGTFILMFSVCGVISSTQLSGGHVGLLEYAVTAGLSVVVVVYSI 96

Query: 83  APISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLTTRP 142
             ISGAH NP+IT+A A+FG FPWS+V  Y  AQT G   AT   + VYG+    + T+P
Sbjct: 97  GHISGAHLNPSITIAFAVFGGFPWSQVPLYITAQTLGATAATLVGVSVYGVNADIMATKP 156

Query: 143 FYSPISAFFLEFLATFILMFLLSSLTYQP-QSIGQLSGFVIGMAVGLAVFIAGPISGASM 202
             S +SAFF+E +AT I++FL S+L   P Q++G L+GFVIG  + L V I GPISG SM
Sbjct: 157 ALSCVSAFFVELIATSIVVFLASALHCGPHQNLGNLTGFVIGTVISLGVLITGPISGGSM 216

Query: 203 NPARSLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPLHSSSSPSDSRL 259
           NPARSLGPA+V+W F D+WIY+TAP+IGA+ G      + L   P  S  SPS S L
Sbjct: 217 NPARSLGPAVVAWDFEDLWIYMTAPVIGAIIGVLTYRSISLKTRPCPSPVSPSVSSL 273

BLAST of Lsi05G014520 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 164.1 bits (414), Expect = 1.6e-40
Identity = 86/219 (39.27%), Postives = 131/219 (59.82%), Query Frame = 0

Query: 30  LARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAH 89
           L + ++ EM+G++ ++    GV V   L GG +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 90  FNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQ-LTTRPFYSPIS 149
           FNPA+T+  AIF  FPW +V  Y  AQ  G ++A+     ++ + P+    T P  SP  
Sbjct: 101 FNPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPAR 160

Query: 150 AFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLG 209
           A   E + +F+LMF++S +    +++G+L+G  +GM + + VF+AGPISGASMNPARSLG
Sbjct: 161 ALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLG 220

Query: 210 PAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
           PA+V   +  IW+YI  P++G ++G F   ++R    PL
Sbjct: 221 PALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPL 259

BLAST of Lsi05G014520 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 158.3 bits (399), Expect = 8.7e-39
Identity = 87/219 (39.73%), Postives = 126/219 (57.53%), Query Frame = 0

Query: 30  LARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAH 89
           L + ++ EM+G++ ++    GV V   L GG +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 90  FNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQ-LTTRPFYSPIS 149
           FNPA+T+  A+F  FPW +V  Y  AQ TG ++A+     ++ + P+    T P  S   
Sbjct: 101 FNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQ 160

Query: 150 AFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPARSLG 209
           A   E + +F+LMF++S +    ++ G+L+G  +GM + L VF+AGPISGASMNPARSLG
Sbjct: 161 ALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLG 220

Query: 210 PAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
           PAIV   +  IW+YI  P +G   G F    +R    PL
Sbjct: 221 PAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPL 259

BLAST of Lsi05G014520 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 148.3 bits (373), Expect = 9.0e-36
Identity = 83/221 (37.56%), Postives = 128/221 (57.92%), Query Frame = 0

Query: 20  FPQPSTMDHNLARPVLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLT 79
           FP P   D +L R +  E VG+F+L+   +   +  Q   G   L+  A  AGL V ++ 
Sbjct: 69  FPSP---DVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIII 128

Query: 80  FCFAPISGAHFNPAITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLT 139
                ISGAH NP++T+A A   HFPW+ V  Y  AQ +  I A++A   V+        
Sbjct: 129 LSTGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGV 188

Query: 140 TRPFYSPISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGA 199
           T P  S   AF LEF+ TFIL+F+++++    +++G+L+G  +G  V L + +AGP +G 
Sbjct: 189 TIPSVSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGG 248

Query: 200 SMNPARSLGPAIVSWAFNDIWIYITAPIIGAVTG-TFFSGV 240
           SMNP R+LGPA+ S  +  +W+Y+ AP +GA++G   ++GV
Sbjct: 249 SMNPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGV 286

BLAST of Lsi05G014520 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 145.2 bits (365), Expect = 7.6e-35
Identity = 83/222 (37.39%), Postives = 125/222 (56.31%), Query Frame = 0

Query: 34  VLGEMVGSFLLMICVSGVTVTGQLPGGQMGLLDYAVAAGLTVGVLTFCFAPISGAHFNPA 93
           ++ E++G++ L+                + L   A+  GLTV VL +    ISGAHFNPA
Sbjct: 54  LMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISGAHFNPA 113

Query: 94  ITLASAIFGHFPWSRVMPYAVAQTTGCIMATYAAIFVYGIKPQQLT--------TRPFYS 153
           +T+A A  G FP  +V  Y ++Q  G  +A      ++G+     +        T P  S
Sbjct: 114 VTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTLPSGS 173

Query: 154 PISAFFLEFLATFILMFLLSSLTYQPQSIGQLSGFVIGMAVGLAVFIAGPISGASMNPAR 213
            + +F +EF+ TF LMF++S +    ++IG+L+G  +G  V L V IAGP+SGASMNP R
Sbjct: 174 NLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGASMNPGR 233

Query: 214 SLGPAIVSWAFNDIWIYITAPIIGAVTGTFFSGVLRLHPPPL 248
           SLGPA+V   +  +WIYI +PI+GAV+G +   ++R    PL
Sbjct: 234 SLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDKPL 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LAI11.1e-5751.48Probable aquaporin NIP7-1 OS=Arabidopsis thaliana OX=3702 GN=NIP7-1 PE=2 SV=2[more]
Q9FIZ92.2e-3939.27Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1[more]
Q8W0361.2e-3739.73Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2[more]
Q407462.1e-3740.00Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-1 PE=2 SV=1[more]
Q9ATN44.7e-3738.14Aquaporin NIP1-1 OS=Zea mays OX=4577 GN=NIP1-1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AX294.1e-11380.74probable aquaporin NIP7-1 OS=Cucumis melo OX=3656 GN=LOC103483738 PE=3 SV=1[more]
A0A0A0LAF02.1e-11280.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149960 PE=3 SV=1[more]
A0A6J1IR001.2e-10777.21probable aquaporin NIP7-1 OS=Cucurbita maxima OX=3661 GN=LOC111479196 PE=3 SV=1[more]
A0A6J1GVJ72.6e-10777.21probable aquaporin NIP7-1 OS=Cucurbita moschata OX=3662 GN=LOC111457868 PE=3 SV=... [more]
A0A6J1C8T11.1e-9276.02probable aquaporin NIP7-1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
Match NameE-valueIdentityDescription
XP_008438714.18.5e-11380.74PREDICTED: probable aquaporin NIP7-1 [Cucumis melo][more]
XP_004134455.24.2e-11280.37probable aquaporin NIP7-1 [Cucumis sativus] >KGN57016.1 hypothetical protein Csa... [more]
KAG6582276.11.1e-10777.57putative aquaporin NIP7-1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022979516.12.4e-10777.21probable aquaporin NIP7-1 [Cucurbita maxima][more]
XP_023528366.12.4e-10777.57probable aquaporin NIP7-1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G06100.17.6e-5951.48NOD26-like intrinsic protein 7;1 [more]
AT5G37810.11.6e-4039.27NOD26-like intrinsic protein 4;1 [more]
AT5G37820.18.7e-3939.73NOD26-like intrinsic protein 4;2 [more]
AT4G10380.19.0e-3637.56NOD26-like intrinsic protein 5;1 [more]
AT4G18910.17.6e-3537.39NOD26-like intrinsic protein 1;2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 220..240
score: 30.55
coord: 71..95
score: 45.63
coord: 108..127
score: 29.85
coord: 32..51
score: 24.04
coord: 152..170
score: 28.07
coord: 183..205
score: 42.81
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 29..235
e-value: 2.6E-47
score: 161.4
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 2..244
e-value: 1.3E-59
score: 203.2
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 27..246
NoneNo IPR availablePANTHERPTHR45724:SF26AQUAPORIN NIP7-1-RELATEDcoord: 3..259
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 3..259
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 89..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G014520.1Lsi05G014520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity