Lsi05G013360 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G013360
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Protein kinase domain-containing protein
Location	chr05: 21143284 .. 21144440 (+)
RNA-Seq Expression	Lsi05G013360
Synteny	Lsi05G013360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTATAAAAAGATTATTGCCTATTTAAAAACCATTATTATGCTTAATTGCTGTGTTGAAGACTAGATTAGTTCAAAGGTTGATTCTACCACTCTTATAAACTATGGAATTTTTTAATAAAAATGTTTTTTTTTATAAATAAAATCGTTTTTTCCTATAAGTATATTTGAAAAAAAAAAATTAATAATTTAAAGTGTATTTATTATAGTTTATTGGAAATAATTTTAGTTGGTCAAAAGTAGTTTTCTGAGAAATTGTAAAAAATAAAAAGTGCTTCAACAAATTGGATTAATTTAAAAACCTTTGGGAAAGAAACACTTGAGAGGTGACTTATTAAAAAAAGTAAATTTAAGGTTATTAGATGGCATCCTCAAAAGTATAAGAAATAAAACATATCAAAATTAAGCATAGCTAAACAGTAATTGACGTACACCGAATTGTACTTAAAAAAAACTTAATAATAAAAACATGATTGATTGTAAGTAAGAGTCTAATAGATCGATCACTTTTGATTACATTGGAACGAAATAAATTTGTTGGTTAAAACAACTTATTAAGAAAAAAAGTAAGGGATTCTAACCAAAACGAATATAACTCAGCTGAAGAGTTTTCTAAAAAATAGCTCTTTTACATTAATCCAAATTTTATAGAGTGCTTTTTTTATTTATTGATTTAAAAAAAAAAAAAATAGTGAAACTATACTGAACACACTTCAAATTACTAATTTTTCCAGTAGAATTTATGGACAATTTTGTTTAAAAAGCATTTTTTTCCGCACGTTAGTCCAAACATACTATTCTACAAATAATAATTGTGTGTTCATATTTCATAAATTTGAAATGCAGAACTTGCATACACAACGGTGGTGACTGAAAAATGTGACGTCTATAGTTTCGGTGTTGTGGCACTTGAAGTTTTAGTAGGAAAACATCCAGGAGATCTCATTCTTTCGTTGCATTCTTCATCTGAAAACAACATTGATCTAAAAGATATTTTAGACTTTCGTCTACAGTTTCCTCAAACGCAAAAGATCATCAACGAGCTATCGATGATAATGAATATCGCGATCTCGTGCATGCAGATGAACCCGCAATCTCGACCGACAATGTACAATGTGAATCGATTGCTGGAAATGCAGGCTGCAGTTGGTTAA

mRNA sequence

ATGGATAGTCTAATAGATCGATCACTTTTGATTACATTGGAACGAAATAAATTTGTTGGAAAACATCCAGGAGATCTCATTCTTTCGTTGCATTCTTCATCTGAAAACAACATTGATCTAAAAGATATTTTAGACTTTCGTCTACAGTTTCCTCAAACGCAAAAGATCATCAACGAGCTATCGATGATAATGAATATCGCGATCTCGTGCATGCAGATGAACCCGCAATCTCGACCGACAATGTACAATGTGAATCGATTGCTGGAAATGCAGGCTGCAGTTGGTTAA

Coding sequence (CDS)

ATGGATAGTCTAATAGATCGATCACTTTTGATTACATTGGAACGAAATAAATTTGTTGGAAAACATCCAGGAGATCTCATTCTTTCGTTGCATTCTTCATCTGAAAACAACATTGATCTAAAAGATATTTTAGACTTTCGTCTACAGTTTCCTCAAACGCAAAAGATCATCAACGAGCTATCGATGATAATGAATATCGCGATCTCGTGCATGCAGATGAACCCGCAATCTCGACCGACAATGTACAATGTGAATCGATTGCTGGAAATGCAGGCTGCAGTTGGTTAA

Protein sequence

MDSLIDRSLLITLERNKFVGKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRPTMYNVNRLLEMQAAVG

Homology

[Close]

BLAST of Lsi05G013360 vs. ExPASy Swiss-Prot
Match: Q9LP24 (Probable leucine-rich repeat receptor-like protein kinase At1g35710 OS=Arabidopsis thaliana OX=3702 GN=At1g35710 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.0e-07
Identity = 29/67 (43.28%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 19   VGKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSR 78
            +GKHPGDL+ SL SS    + L+ I D R+  P+ Q     L M+  +A+ C+Q NP+SR
Sbjct: 1051 IGKHPGDLVSSLSSSPGEALSLRSISDERVLEPRGQNREKLLKMV-EMALLCLQANPESR 1110

Query: 79   PTMYNVN 86
            PTM +++
Sbjct: 1111 PTMLSIS 1116

[Close]

BLAST of Lsi05G013360 vs. ExPASy Swiss-Prot
Match: Q8VZG8 (MDIS1-interacting receptor like kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MIK2 PE=1 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 2.5e-06
Identity = 32/77 (41.56%), Postives = 50/77 (64.94%), Query Frame = 0


Query: 10   LITLERNKFVGKHPGDLILSLHSS-SENNIDLKDILDFRLQFPQTQKIINELSMIMNIAI 69
            ++TLE  K  G+HPGDL+ +L SS  +  + LK I D RL  P T +I  E+  I+ +A+
Sbjct: 968  VLTLEVIK--GEHPGDLVSTLSSSPPDATLSLKSISDHRLPEP-TPEIKEEVLEILKVAL 1027

Query: 70   SCMQMNPQSRPTMYNVN 86
             C+  +PQ+RPTM +++
Sbjct: 1028 LCLHSDPQARPTMLSIS 1041

[Close]

BLAST of Lsi05G013360 vs. ExPASy TrEMBL
Match: A0A6J1CIB4 (MDIS1-interacting receptor like kinase 2-like OS=Momordica charantia OX=3673 GN=LOC111011448 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-23
Identity = 60/77 (77.92%), Postives = 69/77 (89.61%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENN-IDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSR 79
           GKHPGDLIL+LHSSSENN IDLKDILD R  FP+ QK +N+LS+IMN+AISC+ MNP+SR
Sbjct: 881 GKHPGDLILTLHSSSENNDIDLKDILDSRQPFPRIQKTVNDLSLIMNLAISCVCMNPRSR 940

Query: 80  PTMYNVNRLLEMQAAVG 96
           PTMY V+RLLEMQAAVG
Sbjct: 941 PTMYEVSRLLEMQAAVG 957

[Close]

BLAST of Lsi05G013360 vs. ExPASy TrEMBL
Match: A0A0A0L5K3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174580 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.4e-23
Identity = 58/76 (76.32%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           GKHP D+I +LHSS E NIDLKDILD RL+FP+TQKII ELS+IM +AISC+Q  PQSRP
Sbjct: 886 GKHPRDIISTLHSSPEINIDLKDILDCRLEFPETQKIITELSLIMTLAISCVQAKPQSRP 945

Query: 80  TMYNVNRLLEMQAAVG 96
           TMYNV+RLLE+QAAVG
Sbjct: 946 TMYNVSRLLELQAAVG 961

[Close]

BLAST of Lsi05G013360 vs. ExPASy TrEMBL
Match: A0A5D3DJ99 (Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00310 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.1e-23
Identity = 57/76 (75.00%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           GKHP DLI + HSS ENNIDLKDILD RL+FP+T KI+NELS+IM +AISC+Q  PQSRP
Sbjct: 850 GKHPRDLISTWHSSPENNIDLKDILDNRLEFPRTPKIVNELSLIMTLAISCVQAKPQSRP 909

Query: 80  TMYNVNRLLEMQAAVG 96
           TMYNV+RLLE+QA VG
Sbjct: 910 TMYNVSRLLELQATVG 925

[Close]

BLAST of Lsi05G013360 vs. ExPASy TrEMBL
Match: A0A1S3AYI3 (probable LRR receptor-like serine/threonine-protein kinase At4g08850 OS=Cucumis melo OX=3656 GN=LOC103483974 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.3e-23
Identity = 56/76 (73.68%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           GKHP DLI + HSS ENNIDLKDILD RL+FP+T KI+NELS+IM +AISC+Q  PQSRP
Sbjct: 879 GKHPRDLISTWHSSPENNIDLKDILDNRLEFPRTPKIVNELSLIMTLAISCVQAKPQSRP 938

Query: 80  TMYNVNRLLEMQAAVG 96
           TMYNV+RLLE+QA +G
Sbjct: 939 TMYNVSRLLELQATIG 954

[Close]

BLAST of Lsi05G013360 vs. ExPASy TrEMBL
Match: A0A6J1FU43 (MDIS1-interacting receptor like kinase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447310 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.9e-22
Identity = 53/76 (69.74%), Postives = 65/76 (85.53%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           G HPGD+I +L SSS+NNIDLKD+LDFRL FPQTQKI+++L +IMN+ ISC+Q +P SRP
Sbjct: 877 GTHPGDIIFALQSSSDNNIDLKDVLDFRLPFPQTQKIVDDLCLIMNVVISCVQTDPSSRP 936

Query: 80  TMYNVNRLLEMQAAVG 96
           TM NVN LLE+Q AVG
Sbjct: 937 TMSNVNWLLEVQGAVG 952

[Close]

BLAST of Lsi05G013360 vs. NCBI nr
Match: XP_038905199.1 (MDIS1-interacting receptor like kinase 2-like [Benincasa hispida])

HSP 1 Score: 121.7 bits (304), Expect = 3.4e-24
Identity = 62/76 (81.58%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 19  VGKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSR 78
           VGK+  D IL+LHSSS NNIDLKDILD RL FPQ QKIINELS+IMN+AISC+Q NPQSR
Sbjct: 887 VGKYTKDFILTLHSSSGNNIDLKDILDSRLPFPQMQKIINELSLIMNLAISCVQTNPQSR 946

Query: 79  PTMYNVNRLLEMQAAV 95
           PTMYNVNRLLEM AAV
Sbjct: 947 PTMYNVNRLLEMHAAV 962

[Close]

BLAST of Lsi05G013360 vs. NCBI nr
Match: XP_022140892.1 (MDIS1-interacting receptor like kinase 2-like [Momordica charantia])

HSP 1 Score: 118.6 bits (296), Expect = 2.9e-23
Identity = 60/77 (77.92%), Postives = 69/77 (89.61%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENN-IDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSR 79
           GKHPGDLIL+LHSSSENN IDLKDILD R  FP+ QK +N+LS+IMN+AISC+ MNP+SR
Sbjct: 881 GKHPGDLILTLHSSSENNDIDLKDILDSRQPFPRIQKTVNDLSLIMNLAISCVCMNPRSR 940

Query: 80  PTMYNVNRLLEMQAAVG 96
           PTMY V+RLLEMQAAVG
Sbjct: 941 PTMYEVSRLLEMQAAVG 957

[Close]

BLAST of Lsi05G013360 vs. NCBI nr
Match: XP_004148167.1 (MDIS1-interacting receptor like kinase 2 [Cucumis sativus] >KGN57255.1 hypothetical protein Csa_010269 [Cucumis sativus])

HSP 1 Score: 117.9 bits (294), Expect = 4.9e-23
Identity = 58/76 (76.32%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           GKHP D+I +LHSS E NIDLKDILD RL+FP+TQKII ELS+IM +AISC+Q  PQSRP
Sbjct: 886 GKHPRDIISTLHSSPEINIDLKDILDCRLEFPETQKIITELSLIMTLAISCVQAKPQSRP 945

Query: 80  TMYNVNRLLEMQAAVG 96
           TMYNV+RLLE+QAAVG
Sbjct: 946 TMYNVSRLLELQAAVG 961

[Close]

BLAST of Lsi05G013360 vs. NCBI nr
Match: KAA0067661.1 (putative LRR receptor-like serine/threonine-protein kinase [Cucumis melo var. makuwa] >TYK23665.1 putative LRR receptor-like serine/threonine-protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 8.4e-23
Identity = 57/76 (75.00%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           GKHP DLI + HSS ENNIDLKDILD RL+FP+T KI+NELS+IM +AISC+Q  PQSRP
Sbjct: 850 GKHPRDLISTWHSSPENNIDLKDILDNRLEFPRTPKIVNELSLIMTLAISCVQAKPQSRP 909

Query: 80  TMYNVNRLLEMQAAVG 96
           TMYNV+RLLE+QA VG
Sbjct: 910 TMYNVSRLLELQATVG 925

[Close]

BLAST of Lsi05G013360 vs. NCBI nr
Match: XP_008439079.1 (PREDICTED: probable LRR receptor-like serine/threonine-protein kinase At4g08850 [Cucumis melo])

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-22
Identity = 56/76 (73.68%), Postives = 66/76 (86.84%), Query Frame = 0


Query: 20  GKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSRP 79
           GKHP DLI + HSS ENNIDLKDILD RL+FP+T KI+NELS+IM +AISC+Q  PQSRP
Sbjct: 879 GKHPRDLISTWHSSPENNIDLKDILDNRLEFPRTPKIVNELSLIMTLAISCVQAKPQSRP 938

Query: 80  TMYNVNRLLEMQAAVG 96
           TMYNV+RLLE+QA +G
Sbjct: 939 TMYNVSRLLELQATIG 954

[Close]

BLAST of Lsi05G013360 vs. TAIR 10
Match: AT1G35710.1 (Protein kinase family protein with leucine-rich repeat domain )

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-08
Identity = 29/67 (43.28%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 19   VGKHPGDLILSLHSSSENNIDLKDILDFRLQFPQTQKIINELSMIMNIAISCMQMNPQSR 78
            +GKHPGDL+ SL SS    + L+ I D R+  P+ Q     L M+  +A+ C+Q NP+SR
Sbjct: 1051 IGKHPGDLVSSLSSSPGEALSLRSISDERVLEPRGQNREKLLKMV-EMALLCLQANPESR 1110

Query: 79   PTMYNVN 86
            PTM +++
Sbjct: 1111 PTMLSIS 1116

[Close]

BLAST of Lsi05G013360 vs. TAIR 10
Match: AT4G08850.1 (Leucine-rich repeat receptor-like protein kinase family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.8e-07
Identity = 32/77 (41.56%), Postives = 50/77 (64.94%), Query Frame = 0


Query: 10   LITLERNKFVGKHPGDLILSLHSS-SENNIDLKDILDFRLQFPQTQKIINELSMIMNIAI 69
            ++TLE  K  G+HPGDL+ +L SS  +  + LK I D RL  P T +I  E+  I+ +A+
Sbjct: 968  VLTLEVIK--GEHPGDLVSTLSSSPPDATLSLKSISDHRLPEP-TPEIKEEVLEILKVAL 1027

Query: 70   SCMQMNPQSRPTMYNVN 86
             C+  +PQ+RPTM +++
Sbjct: 1028 LCLHSDPQARPTMLSIS 1041

The following BLAST results are available for this feature:

BLAST of Lsi05G013360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LP24	3.0e-07	43.28	Probable leucine-rich repeat receptor-like protein kinase At1g35710 OS=Arabidops...	[more]
Q8VZG8	2.5e-06	41.56	MDIS1-interacting receptor like kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MIK2...	[more]

BLAST of Lsi05G013360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIB4	1.4e-23	77.92	MDIS1-interacting receptor like kinase 2-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A0A0L5K3	2.4e-23	76.32	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174...	[more]
A0A5D3DJ99	4.1e-23	75.00	Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. ...	[more]
A0A1S3AYI3	5.3e-23	73.68	probable LRR receptor-like serine/threonine-protein kinase At4g08850 OS=Cucumis ...	[more]
A0A6J1FU43	5.9e-22	69.74	MDIS1-interacting receptor like kinase 2-like isoform X1 OS=Cucurbita moschata O...	[more]

BLAST of Lsi05G013360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905199.1	3.4e-24	81.58	MDIS1-interacting receptor like kinase 2-like [Benincasa hispida]	[more]
XP_022140892.1	2.9e-23	77.92	MDIS1-interacting receptor like kinase 2-like [Momordica charantia]	[more]
XP_004148167.1	4.9e-23	76.32	MDIS1-interacting receptor like kinase 2 [Cucumis sativus] >KGN57255.1 hypotheti...	[more]
KAA0067661.1	8.4e-23	75.00	putative LRR receptor-like serine/threonine-protein kinase [Cucumis melo var. ma...	[more]
XP_008439079.1	1.1e-22	73.68	PREDICTED: probable LRR receptor-like serine/threonine-protein kinase At4g08850 ...	[more]

BLAST of Lsi05G013360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G35710.1	2.1e-08	43.28	Protein kinase family protein with leucine-rich repeat domain	[more]
AT4G08850.1	1.8e-07	41.56	Leucine-rich repeat receptor-like protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 9..94 e-value: 1.5E-5 score: 26.2
None	No IPR available	PANTHER	PTHR48053	LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED	coord: 18..88
None	No IPR available	PANTHER	PTHR48053:SF2	FLAGELLIN-SENSING-LIKE PROTEIN	coord: 18..88

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G013360.1	Lsi05G013360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009755	hormone-mediated signaling pathway
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity