Lsi05G013210 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G013210
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPectinesterase
Locationchr05: 20974152 .. 20980081 (+)
RNA-Seq ExpressionLsi05G013210
SyntenyLsi05G013210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTTTGTCCTTTACAGTGTTGAAACTTTCATTTATACAATACAGTTTAAAATAATGTTTTTATTTATGGCAACAAACATTTTATTTCTAAAAATTGTAACATTTTAAATAGGAGTAATTGTGTTTTTGCACTAGCTGAGAATAAGATCATGCATTAAATTTTTGTCAGACATCTCTATGTTTTTTTTTTATCAATATTAGATATTAACGTGAGGGGTGAGGGGTGAGTGAACATTTTTATTATACGTAAAAAAAATCTACAAAACATAACAAAATAAGAAAAAGAAAAAAAACGTTAGCGATATAAATAATAAACATGTTAACTTATTTAAAAATTCTAAAAAATTTATTTATTATTATTTTTTAATTTTTTTACTTTTAATATCTTTTTAGAAATATTCATCGACATCAACATTTTGTCGATATTTTTATCGACATTTCTTTAAAATTAAGAATTCGATATTTTTATTGATATTCACATTTTAAACATTGAATAAGATTAATATTAAATATTAGAAATAAAATCACTATCTACTATAAAATAATAGTTTTTAATGGTTACATCATCATTGCAATAATTAAGTTCTTGTGCCAACAGAGTCGAGCACAACTAGTATTTGTATGTGCTAAAGACTAAAAGGTATGTACTTTAAATATCCCAATTTCCATTTGTACCAAAGAAAAAAATAATTAAGGTTGTATTTAGTAATAATTTTATTTTTAGTTTTTGATAACTAAGCATATTTCCTCTTAATTTATTACAATAATTTACATATTTAAGTACAATAGTTGAATTATTAGTCAAATTTCAAATACAAAAACTTTAGTTTTTTAAATTGTACTTTGTTTTTTAAAACATAAAAAGTAGATAATAAAACAAAAAATTTAGAAGTGAAAGGAATATTTATAGATTTAATTTTCAAAAATAAAAAAAATAAAAAACTAAATAATTACCAAACGAAGTCTAAATATCTAACCCCATAATAGATTTGATTGAAAAGATTAAAGGTTTTAAACATTGTGGCAATGGCATATATATGCATGCCTTATAAATTTCCCTATATATATCTCATTTCTATTATTGAAAATATATATATATATCTTAAAGGTTAGGCAAGCAATCTCTGTTAAGTTAGTCAAAGTCAACTCCATATATACAACTGTTTGCATGAAGCTGAAGACATTATAAAATTTTAATTGTAATTCAACTCTCTCATCTTCTATAAGGTAGGTGGAGAAGGGAAAAGGCCTGTGAAGTGAATAGAATGATTGGCACATGGAGATGATACAACTTATGAAGTTTGATTATTCAAACTAATATTATTATATTTTCTTCACATACCCAACCTGTCTCCATATCATATCCCAATTTCCTCTTGATCTTCCCTATATAAAGTTATAAACTAAACTTGAGAGAGCTATAGCTTTAGCTTAACCAAGAAATAATTAGTCTTTCAATGGAGAAATTAAGTCTTGGCCGGGTGTTCCTTTTTCTTCACTTCTTTCTACTATCTTCAACAACCTTTTCCAAGCCTACCACTAATGGCCTTATTGATTGGTGGTGCAAAAAAACCACATACCCTGAAACTTGCAAATACTTTTTCAACCATGGCAGCAAGGCTACTCCCAAAGATATGGCTGAGTTCAAGAAAATGGTGATCCAATTTGCCATGGAAACCGCTCTCAATGCTGAGAGCTACACTAAAGATGTCGGTTCGATGTGTCGAAATGGGAAAGAAAAGGCAGCTTGGACTGATTGCTTGAAGCTATACCAAAACACCATCTTTCAACTCAACCAAACTCTATATTCCTCTACTACTTGTACTGATTTCGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTCGAAACGTGTCGTACCGGGTTTGCCGAGCTTAATGTTTCAGACTATATATTACCGATGATCATGTCGAACAATGTCACGAAACTGATAAGCAATAGTTTGGCTATTAATAACAATGCTTCAAGTGGGGTAATGGGGAAGGAAACATATAAAAAGGGATTTCCAAGTTGGGTTTCAAGTGGTGATAGGAGGTTATTGCAATCGTCGTCGATAAAGGTGGATGTTGTGGTGGCTCGAGACGGATCGGGTAACTATAGGACGGTGGGAGAAGCTGTAGAGGAAGCTGGGAAAAGGAAGGGAAATGGGAGATTTGTTATAAAAGTAAAGAGAGGGGTTTATGAAGAAAATGTGGAGATAGGAAGTAAGATGAAGAATATAATGCTGATTGGAGATGGAATGAGATTGACTTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGGTAACTCTCTTTCTCTTTAGGTTGACTGTTTAATTATATTTATAACAACCATTTGAACATGTCAACCTCCATATTTTTCTCATTTTAACATTTCAAATGTGCATATTTGACATTGCCATCTTTGTCATCTAGAATGAAGTTGATTTCTTTCATTCCAAGGCATGTATGGCTTAGAATTTTCTCCTATCCTCCATTTCCAAATAAAAATGTAAGTTTCTCAAAGCTGTAAATATTTGTGATAGCGGTGTTCAAATGACCCAACAACCCGAACAACTTGGACACTATCCAAAACCCAAACTGTAAGAGTTAGGTTGGGTTAAAGTTTTTATATTTTTGTCGGGTTAGGTCTTGGATTGGATAAAAAAATTCAAGTTGACCAAACTTAACTCGAATTATATATATATATATATATATATATATATATATATATATCTTTTAAGATTGATAAATTTGTTCATTTTTAATGGTTTTTCTTTTAGGNTTGTAATTCAACTCTCTCATCTTCTATAAGGTAGGTGGAGAAGGGAAAAGGCCTGTGAAGTGAATAGAATGATTGGCACATGGAGATGATACAACTTATGAAGTTTGATTATTCAAACTAATATTATTATATTTTCTTCACATACCCAACCTGTCTCCATATCATATCCCAATTTCCTCTTGATCTTCCCTATATAAAGTTATAAACTAAACTTGAGAGAGCTATAGCTTTAGCTTAACCAAGAAATAATTAGTCTTTCAATGGAGAAATTAAGTCTTGGCCGGGTGTTCCTTTTTCTTCACTTCTTTCTACTATCTTCAACAACCTTTTCCAAGCCTACCACTAATGGCCTTATTGATTGGTGGTGCAAAAAAACCACATACCCTGAAACTTGCAAATACTTTTTCAACCATGGCAGCAAGGCTACTCCCAAAGATATGGCTGAGTTCAAGAAAATGGTGATCCAATTTGCCATGGAAACCGCTCTCAATGCTGAGAGCTACACTAAAGATGTCGGTTCGATGTGTCGAAATGGGAAAGAAAAGGCAGCTTGGACTGATTGCTTGAAGCTATACCAAAACACCATCTTTCAACTCAACCAAACTCTATATTCCTCTACTACTTGTACTGATTTCGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTCGAAACGTGTCGTACCGGGTTTGCCGAGCTTAATGTTTCAGACTATATATTACCGATGATCATGTCGAACAATGTCACGAAACTGATAAGCAATAGTTTGGCTATTAATAACAATGCTTCAAGTGGGGTAATGGGGAAGGAAACATATAAAAAGGGATTTCCAAGTTGGGTTTCAAGTGGTGATAGGAGGTTATTGCAATCGTCGTCGATAAAGGTGGATGTTGTGGTGGCTCGAGACGGATCGGGTAACTATAGGACGGTGGGAGAAGCTGTAGAGGAAGCTGGGAAAAGGAAGGGAAATGGGAGATTTGTTATAAAAGTAAAGAGAGGGGTTTATGAAGAAAATGTGGAGATAGGAAGTAAGATGAAGAATATAATGCTGATTGGAGATGGAATGAGATTGACTTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGGTAACTCTCTTTCTCTTTAGGTTGACTGTTTAATTATATTTATAACAACCATTTGAACATGTCAACCTCCATATTTTTCTCATTTTAACATTTCAAATGTGCATATTTGACATTGCCATCTTTGTCATCTAGAATGAAGTTGATTTCTTTCATTCCAAGGCATGTATGGCTTAGAATTTTCTCCTATCCTCCATTTCCAAATAAAAATGTAAGTTTCTCAAAGCTGTAAATATTTGTGATAGCGGTGTTCAAATGACCCAACAACCCGAACAACTTGGACACTATCCAAAACCCAAACTGTAAGAGTTAGGTTGGGTTAAAGTTTTTATATTTTTGTCGGGTTAGGTCTTGGATTGGATAAAAAAATTCAAGTTGACCAAACTTAACTCGAATTATATATATATATATATATATATATATATATATATATATCTTTTAAGATTGATAAATTTGTTCATTTTTAATGGTTTTTCTTTTAGGGACGTTTGTTTTTGTTTAAAGTTTGTAATAGATATCATGAATATTTGATATTTAACATTTGAGTTCAATGAGATTTTAATTATTAGACATGTGTTGTATGGAAATTTAAGTATTTTAAATTAATAAGACAAAAAATAATAACTCAACAATCCAACTCAACCCAACTTTCACAAGTTGTCAACTCGACCAATCCAGTCCCAAAAAAACTCACAACTCAACCTATATACAACATTTCATCATATGCATCATTGGATAAAATGGCAATATATATTGAAAAATTATTATAAACAAAAAAAAAAATCAAACTATTTACAAATATAGAAAAATATTACTTTCTAACGCGGATGGACAGTGTAATTTTTGTATATTTATAAATAGTTTGACTAAATTTTTTATAATTAAAACCATCTTATATCTATGGTATTTCATTGCAGGCATCATCATATACACATATGACTACCAAATGTCAATTAATTATACATACATTTTCACGTTTTATACATTTTTAAAATCATGGTAGAAAATTTCGATCGACAAGAAATTTCATTTTATTTTTGAGAATTTTTTTAAAATTAATTAGGTTAGCTGAATTCAAGTTTTTCAATTTATATGGTGATCTTGATTACATACAATTGTATAGTTTCACACGCCAATATCATTTAAGAATGAAACTTTTTAGATCCATTTTTAGATCCGATATTTCTATTGACGTCAATATTTCGAAGGTTCACTAAAACAGCATCATGAAATTAAATATGATTAATTCTAAATACTTGTTTGTCTTCTGTGACAATCAGCGGTGACCGGTGAAGGATTTATAGCTCAGGGCATCACATTCCAAAACACCGCCGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGATCAGGCTCAGATCTCTCTGTTTTCTACCGCTGTGCCTTCGAAGGTTATCAAGACACACTTTATGTCCATTCACAAAGACAATTCTACAAGGAATGTTATATTTACGGTACAGTAGATTTCATATTTGGCAATGCTGCAGTTGTTTTACAAAACTGCATGCTTTACGCAAGGAAGCCAATGAATGGTCAGAAAAATGCAGTCACTGCTCAAGGACGAACGGACCCAAATCAAAACACTGGCATTTCAATTCATAATTCACGGGTAATGGCCACAGATGAGTTAAAAATAGTGGAGAACACAGTGAAAACTTACTTAGGAAGGCCATGGAAGGAGTATTCTCGAACAGTTTATATGAAAACTTACCTTGATAGTTTGGTTGATCCAATGGGTTGGTTGGAATGGGATGGTGAATTTGCATTGAATACTTTATATTATGGAGAATATAAGAATATTGGTCCTGCTTCATCAACAAGCCAGCGAGTTAATTGGAAAGGTTACCATGTTATTAGTAATGCAACAGAGGCATCGGAGTTTACTGTTCAAAATTTCATTGCAGGGGAGTCTTGGTTGCCAGATACAGAGGTCCCATTCACTCCTGGACTCTAG

mRNA sequence

ATGACTCTTTCAATGGAGAAATTAAGTCTTGGCCGGGTGTTCCTTTTTCTTCACTTCTTTCTACTATCTTCAACAACCTTTTCCAAGCCTACCACTAATGGCCTTATTGATTGGTGGTGCAAAAAAACCACATACCCTGAAACTTGCAAATACTTTTTCAACCATGGCAGCAAGGCTACTCCCAAAGATATGGCTGAGTTCAAGAAAATGGTGATCCAATTTGCCATGGAAACCGCTCTCAATGCTGAGAGCTACACTAAAGATGTCGGTTCGATGTGTCGAAATGGGAAAGAAAAGGCAGCTTGGACTGATTGCTTGAAGCTATACCAAAACACCATCTTTCAACTCAACCAAACTCTATATTCCTCTACTACTTGTACTGATTTCGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTCGAAACGTGTCGTACCGGGTTTGCCGAGCTTAATGTTTCAGACTATATATTACCGATGATCATGTCGAACAATGTCACGAAACTGATAAGCAATAGTTTGGCTATTAATAACAATGCTTCAAGTGGGGTAATGGGGAAGGAAACATATAAAAAGGGATTTCCAAGTTGGGTTTCAAGTGGTGATAGGAGGTTATTGCAATCGTCGTCGATAAAGGTGGATGTTGTGGTGGCTCGAGACGGATCGGGTAACTATAGGACGGTGGGAGAAGCTGTAGAGGAAGCTGGGAAAAGGAAGGGAAATGGGAGATTTGTTATAAAAGTAAAGAGAGGGGTTTATGAAGAAAATGTGGAGATAGGAAGTAAGATGAAGAATATAATGCTGATTGGAGATGGAATGAGATTGACTTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTCTTAACCAAGAAATAATTAGTCTTTCAATGGAGAAATTAAGTCTTGGCCGGGTGTTCCTTTTTCTTCACTTCTTTCTACTATCTTCAACAACCTTTTCCAAGCCTACCACTAATGGCCTTATTGATTGGTGGTGCAAAAAAACCACATACCCTGAAACTTGCAAATACTTTTTCAACCATGGCAGCAAGGCTACTCCCAAAGATATGGCTGAGTTCAAGAAAATGGTGATCCAATTTGCCATGGAAACCGCTCTCAATGCTGAGAGCTACACTAAAGATGTCGGTTCGATGTGTCGAAATGGGAAAGAAAAGGCAGCTTGGACTGATTGCTTGAAGCTATACCAAAACACCATCTTTCAACTCAACCAAACTCTATATTCCTCTACTACTTGTACTGATTTCGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTCGAAACGTGTCGTACCGGGTTTGCCGAGCTTAATGTTTCAGACTATATATTACCGATGATCATGTCGAACAATGTCACGAAACTGATAAGCAATAGTTTGGCTATTAATAACAATGCTTCAAGTGGGGTAATGGGGAAGGAAACATATAAAAAGGGATTTCCAAGTTGGGTTTCAAGTGGTGATAGGAGGTTATTGCAATCGTCGTCGATAAAGGTGGATGTTGTGGTGGCTCGAGACGGATCGGGTAACTATAGGACGGTGGGAGAAGCTGTAGAGGAAGCTGGGAAAAGGAAGGGAAATGGGAGATTTGTTATAAAAGTAAAGAGAGGGGTTTATGAAGAAAATGTGGAGATAGGAAGTAAGATGAAGAATATAATGCTGATTGGAGATGGAATGAGATTGACTTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGCGGTGACCGGTGAAGGATTTATAGCTCAGGGCATCACATTCCAAAACACCGCCGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGATCAGGCTCAGATCTCTCTGTTTTCTACCGCTGTGCCTTCGAAGGTTATCAAGACACACTTTATGTCCATTCACAAAGACAATTCTACAAGGAATGTTATATTTACGGTACAGTAGATTTCATATTTGGCAATGCTGCAGTTGTTTTACAAAACTGCATGCTTTACGCAAGGAAGCCAATGAATGGTCAGAAAAATGCAGTCACTGCTCAAGGACGAACGGACCCAAATCAAAACACTGGCATTTCAATTCATAATTCACGGGTAATGGCCACAGATGAGTTAAAAATAGTGGAGAACACAGTGAAAACTTACTTAGGAAGGCCATGGAAGGAGTATTCTCGAACAGTTTATATGAAAACTTACCTTGATAGTTTGGTTGATCCAATGGGTTGGTTGGAATGGGATGGTGAATTTGCATTGAATACTTTATATTATGGAGAATATAAGAATATTGGTCCTGCTTCATCAACAAGCCAGCGAGTTAATTGGAAAGGTTACCATGTTATTAGTAATGCAACAGAGGCATCGGAGTTTACTGTTCAAAATTTCATTGCAGGGGAGTCTTGGTTGCCAGATACAGAGGTCCCATTCACTCCTGGACTCTAG

Coding sequence (CDS)

ATGACTCTTTCAATGGAGAAATTAAGTCTTGGCCGGGTGTTCCTTTTTCTTCACTTCTTTCTACTATCTTCAACAACCTTTTCCAAGCCTACCACTAATGGCCTTATTGATTGGTGGTGCAAAAAAACCACATACCCTGAAACTTGCAAATACTTTTTCAACCATGGCAGCAAGGCTACTCCCAAAGATATGGCTGAGTTCAAGAAAATGGTGATCCAATTTGCCATGGAAACCGCTCTCAATGCTGAGAGCTACACTAAAGATGTCGGTTCGATGTGTCGAAATGGGAAAGAAAAGGCAGCTTGGACTGATTGCTTGAAGCTATACCAAAACACCATCTTTCAACTCAACCAAACTCTATATTCCTCTACTACTTGTACTGATTTCGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTCGAAACGTGTCGTACCGGGTTTGCCGAGCTTAATGTTTCAGACTATATATTACCGATGATCATGTCGAACAATGTCACGAAACTGATAAGCAATAGTTTGGCTATTAATAACAATGCTTCAAGTGGGGTAATGGGGAAGGAAACATATAAAAAGGGATTTCCAAGTTGGGTTTCAAGTGGTGATAGGAGGTTATTGCAATCGTCGTCGATAAAGGTGGATGTTGTGGTGGCTCGAGACGGATCGGGTAACTATAGGACGGTGGGAGAAGCTGTAGAGGAAGCTGGGAAAAGGAAGGGAAATGGGAGATTTGTTATAAAAGTAAAGAGAGGGGTTTATGAAGAAAATGTGGAGATAGGAAGTAAGATGAAGAATATAATGCTGATTGGAGATGGAATGAGATTGACTTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTCTTAACCAAGAAATAATTAGTCTTTCAATGGAGAAATTAAGTCTTGGCCGGGTGTTCCTTTTTCTTCACTTCTTTCTACTATCTTCAACAACCTTTTCCAAGCCTACCACTAATGGCCTTATTGATTGGTGGTGCAAAAAAACCACATACCCTGAAACTTGCAAATACTTTTTCAACCATGGCAGCAAGGCTACTCCCAAAGATATGGCTGAGTTCAAGAAAATGGTGATCCAATTTGCCATGGAAACCGCTCTCAATGCTGAGAGCTACACTAAAGATGTCGGTTCGATGTGTCGAAATGGGAAAGAAAAGGCAGCTTGGACTGATTGCTTGAAGCTATACCAAAACACCATCTTTCAACTCAACCAAACTCTATATTCCTCTACTACTTGTACTGATTTCGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTCGAAACGTGTCGTACCGGGTTTGCCGAGCTTAATGTTTCAGACTATATATTACCGATGATCATGTCGAACAATGTCACGAAACTGATAAGCAATAGTTTGGCTATTAATAACAATGCTTCAAGTGGGGTAATGGGGAAGGAAACATATAAAAAGGGATTTCCAAGTTGGGTTTCAAGTGGTGATAGGAGGTTATTGCAATCGTCGTCGATAAAGGTGGATGTTGTGGTGGCTCGAGACGGATCGGGTAACTATAGGACGGTGGGAGAAGCTGTAGAGGAAGCTGGGAAAAGGAAGGGAAATGGGAGATTTGTTATAAAAGTAAAGAGAGGGGTTTATGAAGAAAATGTGGAGATAGGAAGTAAGATGAAGAATATAATGCTGATTGGAGATGGAATGAGATTGACTTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGCGGTGACCGGTGAAGGATTTATAGCTCAGGGCATCACATTCCAAAACACCGCCGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGATCAGGCTCAGATCTCTCTGTTTTCTACCGCTGTGCCTTCGAAGGTTATCAAGACACACTTTATGTCCATTCACAAAGACAATTCTACAAGGAATGTTATATTTACGGTACAGTAGATTTCATATTTGGCAATGCTGCAGTTGTTTTACAAAACTGCATGCTTTACGCAAGGAAGCCAATGAATGGTCAGAAAAATGCAGTCACTGCTCAAGGACGAACGGACCCAAATCAAAACACTGGCATTTCAATTCATAATTCACGGGTAATGGCCACAGATGAGTTAAAAATAGTGGAGAACACAGTGAAAACTTACTTAGGAAGGCCATGGAAGGAGTATTCTCGAACAGTTTATATGAAAACTTACCTTGATAGTTTGGTTGATCCAATGGGTTGGTTGGAATGGGATGGTGAATTTGCATTGAATACTTTATATTATGGAGAATATAAGAATATTGGTCCTGCTTCATCAACAAGCCAGCGAGTTAATTGGAAAGGTTACCATGTTATTAGTAATGCAACAGAGGCATCGGAGTTTACTGTTCAAAATTTCATTGCAGGGGAGTCTTGGTTGCCAGATACAGAGGTCCCATTCACTCCTGGACTCTAG

Protein sequence

MTLSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATLNQEIISLSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYYGEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL
Homology
[Close]
BLAST of Lsi05G013210 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.6e-173
Identity = 310/512 (60.55%), Postives = 386/512 (75.39%), Query Frame = 0 

Query: 318 LHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATP-KDMAEFKKMVIQFA 377
           L  F LS T+F        +  WC KT  P+ C+YF    +  T  K   +F K+ +Q A
Sbjct: 11  LVLFSLSHTSFGYSPEE--VKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLA 70

Query: 378 METALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWL 437
           +E A  A+S T  +GS CRN +EKAAW DC +LY+ T+ +LNQT  SS  CT  D QTWL
Sbjct: 71  LERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWL 130

Query: 438 STALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGF 497
           STALTNLETCR    +L V +Y+LP ++SNNVTKLISN+L++N         + +YK GF
Sbjct: 131 STALTNLETCRASLEDLGVPEYVLP-LLSNNVTKLISNTLSLNKVP----YNEPSYKDGF 190

Query: 498 PSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYE 557
           P+WV  GDR+LLQ++  + ++VVA+DGSGN +T+ EAV  A  R G  R+VI +K G Y 
Sbjct: 191 PTWVKPGDRKLLQTTP-RANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYN 250

Query: 558 ENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTA 617
           EN+E+  K+KNIM +GDG+  T ITG++SVGGG+TTF SATVAV G+ FIA+ IT +NTA
Sbjct: 251 ENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTA 310

Query: 618 GPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 677
           GP NHQAVALRSGSDLSVFYRC+FEGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVL
Sbjct: 311 GPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVL 370

Query: 678 QNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELKIVENTVKTYLGRPW 737
           QNC ++ARKP N + N +TAQGRTDPNQ+TGI IHN RV A  +LK V+++VKT+LGRPW
Sbjct: 371 QNCNIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPW 430

Query: 738 KEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYYGEYKNIGPASSTSQRVNWKGYHVI 797
           K+YSRTVY+KT+LDSL++P GW+EW G+FALNTLYY EY N GP SST+ RV W+GYHV+
Sbjct: 431 KQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVL 490

Query: 798 SNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           ++ ++ S+FTV NFIAG SWLP T VPFT GL
Sbjct: 491 TSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]
BLAST of Lsi05G013210 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 582.4 bits (1500), Expect = 8.0e-165
Identity = 290/490 (59.18%), Postives = 374/490 (76.33%), Query Frame = 0 

Query: 340 WCKKTTYPETCKYFFNHGSKATP-KDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGK 399
           WC +T  P+ C+YF  H S   P K  +EF K+ ++  ++ A+ A+++   +G  CR+ +
Sbjct: 33  WCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTR 92

Query: 400 EKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDY 459
           EKAAW DC+KLY  T+ ++N+T+  +  C+  D QTWLSTALTNL+TCR GF EL V+D 
Sbjct: 93  EKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDI 152

Query: 460 ILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSSSIKVDVV 519
           +LP +MSNNV+ L+ N+LAIN    +    +   K GFPSWV  GDR+LLQSS+ K + V
Sbjct: 153 VLP-LMSNNVSNLLCNTLAINKVPFNYTPPE---KDGFPSWVKPGDRKLLQSSTPKDNAV 212

Query: 520 VARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLT 579
           VA+DGSGN++T+ EA++ A    G+GRFVI VK+GVY EN+EI  + KN+ML GDG+  T
Sbjct: 213 VAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKT 272

Query: 580 FITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRC 639
            ITG++SVGGG+TTFNSATVA  G+GFIA+GITF+NTAG  N QAVALRSGSDLSVFY+C
Sbjct: 273 IITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQC 332

Query: 640 AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQG 699
           +FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VLQNC ++AR+P   + N +TAQG
Sbjct: 333 SFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQG 392

Query: 700 RTDPNQNTGISIHNSRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGW 759
           R+DPNQNTGI IHNSRV A  +L+ V  + KTYLGRPW++YSRTV+MKT LDSL+DP GW
Sbjct: 393 RSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGW 452

Query: 760 LEWDGEFALNTLYYGEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLP 819
           LEWDG FAL TL+Y E++N GP +STS RV W G+ V+ +A+EAS+FTV  F+AG SW+P
Sbjct: 453 LEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIP 511

Query: 820 DTEVPFTPGL 829
            + VPFT GL
Sbjct: 513 -SSVPFTSGL 511

[Close]
BLAST of Lsi05G013210 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 5.4e-161
Identity = 294/543 (54.14%), Postives = 389/543 (71.64%), Query Frame = 0 

Query: 302 ISLSMEKLSLGRVFLFLHFFLLSSTTFSKPTT---NGLIDWWCKKTTYPETCK-YFFNHG 361
           +++ M+KLS+    LFLH  LL        TT       D WC KT YP+ CK YF NH 
Sbjct: 1   MNMMMQKLSI----LFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHN 60

Query: 362 SKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQL 421
               P  ++EF+ M+++ AM+ A++A +   + G  C + K++A   DC+ LY +TI QL
Sbjct: 61  GFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQL 120

Query: 422 NQTLY-------SSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTK 481
           N+TL+       ++ +CTDFD QTWLSTALTN ETCR G ++LNV+D+I P++ +  ++ 
Sbjct: 121 NRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISH 180

Query: 482 LISNSLAINNN-ASSGVMGKET-YKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYR 541
           LISN LA+N    ++G  G  T  +KGFP+W+S  D+RLL+  +++ ++VVA+DGSG++ 
Sbjct: 181 LISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFN 240

Query: 542 TVGEAVEEAGKRK-GNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVG 601
           TV  A++ AG+RK  +GRFVI VKRG+Y+EN+ +     +IML+GDGMR T ITG RSV 
Sbjct: 241 TVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQ 300

Query: 602 GGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTL 661
           GG TT+NSAT  + G  FIA+GITF+NTAGP   QAVALRS SDLS+FY+C+ EGYQDTL
Sbjct: 301 GGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTL 360

Query: 662 YVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTG 721
            VHSQRQFY+ECYIYGTVDFIFGNAA V QNC++  R+P+ GQ N +TAQGR DP QNTG
Sbjct: 361 MVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTG 420

Query: 722 ISIHNSRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEW--DGEF 781
           ISIHNSR++   +LK V  TVKTY+GRPW ++SRTV ++TYLD++V P+GW  W     F
Sbjct: 421 ISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVF 480

Query: 782 ALNTLYYGEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFT 829
            L+TL+Y EYKN GPASST  RV+WKG+HV+  A++AS FTV  FIAG +WLP T +PFT
Sbjct: 481 GLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFT 536

[Close]
BLAST of Lsi05G013210 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 6.2e-157
Identity = 290/530 (54.72%), Postives = 370/530 (69.81%), Query Frame = 0 

Query: 315 FLFLHFFLLSSTTFSKPTT-----NGLIDWWCKKTTYPETCK-YFFNHGSKATPKDMAEF 374
           FL LH  L+        TT     +  ID WC KT YP  CK YF  H     P  ++EF
Sbjct: 11  FLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70

Query: 375 KKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTL--YSSTT 434
           + ++++ AM+ A++A     +    C + K++A   DC+ LY +T+ QLN+TL   SS T
Sbjct: 71  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130

Query: 435 ---CTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNAS 494
              CTDFD QTWLSTALTN ETCR G ++LNVSD+  P++ +  ++ LISN LA+N    
Sbjct: 131 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALL 190

Query: 495 SGVMGKETY--KKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRK 554
           +      T    KGFP+WVS  +RRLLQ  S++ ++VVA+DGSG+++TV  A++ AG+RK
Sbjct: 191 TAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRK 250

Query: 555 -GNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAV 614
             +GRFVI VKRG+Y+EN+ +     NIML+GDGMR T ITG RSV GG TT++SAT  +
Sbjct: 251 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

Query: 615 TGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECY 674
            G  FIA+GI FQNTAGP   QAVALRS SDLS+FYRC+ EGYQDTL VHSQRQFY+ECY
Sbjct: 311 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 370

Query: 675 IYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDE 734
           IYGTVDFIFGNAAVV QNC++  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   +
Sbjct: 371 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 430

Query: 735 LKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEW--DGEFALNTLYYGEYKNI 794
           LK V  +VKTY+GRPW  YSRTV +KTY+DS+V P+GW  W     + L+TL+Y EYKNI
Sbjct: 431 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 490

Query: 795 GPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           GPASST  RV WKG+HV+S A++AS F+V  FIAG +WLP + +PFT  L
Sbjct: 491 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]
BLAST of Lsi05G013210 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.0e-151
Identity = 273/521 (52.40%), Postives = 359/521 (68.91%), Query Frame = 0 

Query: 332 TTNGLIDWWCKKTTYPETCKYFFNHGSK-----ATPKDMAEFKKMVIQFAMETALNAESY 391
           + +G + WWC KT + ETC Y+F   S+       P+  +EF +M+++ A++ A+   S 
Sbjct: 79  SVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQ 138

Query: 392 TKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTL-------YSSTTCTDFDIQTWLSTA 451
           T   G  C N + KAAW+DC+ L+QNT+ QLN+TL        S   CTDFD QTWLSTA
Sbjct: 139 TVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTA 198

Query: 452 LTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKE-------TY 511
            TN+ETCR+G  +LNVSD+++P+I + N++ LI N LA+N     GV+ K+        +
Sbjct: 199 QTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVN-----GVLMKQHDHTTTANH 258

Query: 512 KKGFPSWVSSGDRRLLQSSSI---KVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIK 571
           K+ FPSWVS  +RRLL S+S+      +VVA+D SG++R++  A+  A +R+   RFVI 
Sbjct: 259 KEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIY 318

Query: 572 VKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQG 631
           VK+GVY EN+++G+   NIML+GDG R T IT  RSV  G TT+NSAT    G+ F+A+ 
Sbjct: 319 VKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKD 378

Query: 632 ITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 691
           +TF NTAGP   QAVA+RS SDLSVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIF
Sbjct: 379 MTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIF 438

Query: 692 GNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELKIVENTVK 751
           GNAAVV QNCM+  R+P++GQ N +TAQGR DP QNTGI+IH+SR++A  +LK V    K
Sbjct: 439 GNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYK 498

Query: 752 TYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEW--DGEFALNTLYYGEYKNIGPASSTSQR 811
           TYLGRPW+ YSR   MKTY+D+ + P+GW  W     FALNT++YGEYKN GP SST  R
Sbjct: 499 TYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWR 558

Query: 812 VNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           V WKG+H I++   AS FTV + IAG SWLP T VPF  GL
Sbjct: 559 VRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]
BLAST of Lsi05G013210 vs. ExPASy TrEMBL
Match: A0A2H5PMQ8 (Pectinesterase OS=Citrus unshiu OX=55188 GN=CUMW_150710 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 8.4e-266
Identity = 523/1056 (49.53%), Postives = 641/1056 (60.70%), Query Frame = 0 

Query: 3    LSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHG-SKATP 62
            ++ E +SL  +F+ L      + +     TN  I WWC KT +PE CKYF +    + T 
Sbjct: 1    MAKENISLLIIFISLSSIFYPALSRRPSATN--ITWWCSKTPHPEPCKYFLSRSHHRYTF 60

Query: 63   KDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLY 122
            K  ++F+K+++Q A+E A  A     + G+ C N  +KAAW+DC+KLY NT+ QLN+TL 
Sbjct: 61   KHRSDFRKIIVQVAIEQAHKAHDQVMEFGTNCENKHQKAAWSDCVKLYANTVLQLNRTLQ 120

Query: 123  ---SSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAIN 182
               S  +CTDFD QTWLSTALTN+ TC++G+ ELNVSD+I P +MSNN+++LISNSLA+N
Sbjct: 121  GLKSDRSCTDFDAQTWLSTALTNIRTCQSGYMELNVSDFITP-VMSNNLSQLISNSLAVN 180

Query: 183  NNASSGVMGKE---TYKKGFPSWVSSGDRRLLQSSSI--KVDVVVARDGSGNYRTVGEAV 242
                 GV+ K    TY  GFPSW+S  +R LL+SSS+  + ++VVA DGSGNYRT+  A+
Sbjct: 181  -----GVLLKRENVTYTNGFPSWLSGHERNLLESSSLEARANLVVATDGSGNYRTIQAAI 240

Query: 243  EEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFN 302
              A +R+G+GRF+I VKRGVY EN+E+G    NIML+G+GMR T IT  +SVG GSTT++
Sbjct: 241  NAAARRRGSGRFIIHVKRGVYRENIEVGLNNNNIMLVGEGMRNTIITSGQSVGSGSTTYS 300

Query: 303  SATL----------------------NQEIISLSMEKLS--------------------- 362
            SAT                        Q +   S   LS                     
Sbjct: 301  SATAGIDGLHFMGRDITFQNTAGPLKGQAVALRSASDLSVFYRCAFQGYQDTLMVHSQRQ 360

Query: 363  ------------------------------------------------------------ 422
                                                                        
Sbjct: 361  FYKKCYIYGTIDFIFGNAAVVFQNCIIFVRKPLKGQANVITAQGRNDPFQNTAISIHSSR 420

Query: 423  -----------------LGR---------------------------------------- 482
                             LGR                                        
Sbjct: 421  VLPANDLKPVVRNFKTYLGRPWQQYSRTVILKTYIDGFVSPLGWSTWSPGNNFALDTLFY 480

Query: 483  -----------------------------------------------------------V 542
                                                                        
Sbjct: 481  GEYENYGPGSSTRHRVKWRGFHVITSPKVASQFTVGSLIAGRSWLPATASALMAMKLSVF 540

Query: 543  FLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSK--ATPKDMAEFKKMV 602
             LF+      +   S   +NG +D+WC KT +PE CKYF    SK  A PK  +EF++M 
Sbjct: 541  LLFISLISFFAPALSSVASNG-VDYWCSKTPHPEPCKYFMQQNSKHFAVPKQKSEFRRMA 600

Query: 603  IQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDI 662
            +  A++ AL A+++ K +GS CRN KEK AW DCLKLYQ+TI QLN TL S+T CTDFD 
Sbjct: 601  MSLALDRALTAQNHNKWLGSKCRNHKEKVAWADCLKLYQDTINQLNHTLDSNTKCTDFDA 660

Query: 663  QTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETY 722
            QTWLSTALTNLETCR GF EL V DY+LP +MSNNVTKLISN+LA+ N ++      ETY
Sbjct: 661  QTWLSTALTNLETCRAGFVELGVPDYVLP-LMSNNVTKLISNTLALRNASTV----PETY 720

Query: 723  KKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKR 782
            K GFPSWV  GDR+LLQ+S ++ ++VVA+DGSGNYRT+  A++ A KR G+GRFVI VKR
Sbjct: 721  KGGFPSWVKPGDRKLLQTSPVRPNLVVAQDGSGNYRTIKAALDAAAKRTGSGRFVIHVKR 780

Query: 783  GVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITF 829
            GVY+EN+EIG+KMKNIML+GDG+R T ITG+RSVGGG TTFNSATVAVTG+GFIA+GITF
Sbjct: 781  GVYKENLEIGNKMKNIMLVGDGLRYTIITGSRSVGGGFTTFNSATVAVTGDGFIARGITF 840

[Close]
BLAST of Lsi05G013210 vs. ExPASy TrEMBL
Match: A0A5D3DJL7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 3.0e-263
Identity = 459/526 (87.26%), Postives = 488/526 (92.78%), Query Frame = 0 

Query: 304 LSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATPK 363
           LSME  SL  VFLFLHFFLLSS+ FSKP +NG IDWWCKKTTYPETCKYFFNHG+K+ PK
Sbjct: 4   LSME-TSLVGVFLFLHFFLLSSSAFSKPASNG-IDWWCKKTTYPETCKYFFNHGTKSPPK 63

Query: 364 DMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYS 423
           DM +FKKMVIQFAME AL+AES+TK VGS CRNGKEKAAW DCLKLYQNTI QLNQTL S
Sbjct: 64  DMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDS 123

Query: 424 STTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNAS 483
           ST CT+FDIQTWLSTALTNLETCRTGFAELNV DYI P+IMS+NVTKLISNSLAI NNAS
Sbjct: 124 STKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAI-NNAS 183

Query: 484 SGV-MGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKG 543
           +GV  GK TYKKGFPSW+S GDRRLLQS S KVD+VVA+DGSGNY TVG A+EEA KRK 
Sbjct: 184 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 243

Query: 544 NGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTG 603
           +GRFVI+VKRGVY EN+EIGSKMKNIMLIGDGMR TFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 244 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 303

Query: 604 EGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 663
           EGFIA+GITFQNTAGPENHQAVALRSG+DLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 304 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 363

Query: 664 GTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELK 723
           GTVDFIFGNAAVVLQNCM+YARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATD+LK
Sbjct: 364 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 423

Query: 724 IVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYYGEYKNIGPAS 783
            VE+TVKTYLGRPWKEYSRTV+MKTY+DSLVDP GWLEWDG+FALNTLYYGEY NIGP S
Sbjct: 424 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 483

Query: 784 STSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
             SQRV WKGYHVISN+TEASEFTVQNFIAG+SWLP+TEVPFTPGL
Sbjct: 484 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]
BLAST of Lsi05G013210 vs. ExPASy TrEMBL
Match: A0A0A0L9G4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 3.3e-262
Identity = 461/536 (86.01%), Postives = 495/536 (92.35%), Query Frame = 0 

Query: 295 ATLNQEIISLSMEKLSLGRVFLFLHFFLLSSTT-FSKPTTNGLIDWWCKKTTYPETCKYF 354
           A+ N E+  LSM   S G VF+FLHFFLLSS++ FSKP +NG IDWWCKKTTYPETCKYF
Sbjct: 40  ASSNIEMAGLSM-GTSFG-VFIFLHFFLLSSSSAFSKPASNG-IDWWCKKTTYPETCKYF 99

Query: 355 FNHGSKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNT 414
           FNHG+K+ PKDM +FKKMVIQFAME AL+AES+TK VGS CRNGKEKAAW DCLKLYQNT
Sbjct: 100 FNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNT 159

Query: 415 IFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLIS 474
           I QLNQTL SST  T+FDIQTWLSTALTNLETCRTGFAELNVSDYILP+IMS+NVT+LIS
Sbjct: 160 ILQLNQTLDSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELIS 219

Query: 475 NSLAINNNASSGV-MGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGE 534
           NSLAI NNAS+GV  GKETYKKGFPSW+S GDRRLLQSSS KVD+VVA+DGSGNY TVG 
Sbjct: 220 NSLAI-NNASAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGA 279

Query: 535 AVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTT 594
           A+EEA KRK +GRFVI+VKRGVY EN+EIGSKMKNIMLIGDGMR TFITGNRSVGGGSTT
Sbjct: 280 ALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTT 339

Query: 595 FNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQ 654
           FNSATVAVTGEGFIA+GITF+NTAGPENHQAVALRSG+DLSVFYRCAFEGYQDTLYVHSQ
Sbjct: 340 FNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQ 399

Query: 655 RQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHN 714
           RQFYKECYIYGTVDFIFGNAAVVLQNCM+YARKPMNGQKNAVTAQGRTDPNQNTGISIHN
Sbjct: 400 RQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHN 459

Query: 715 SRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYY 774
           SRVMATD+LK VE+TVKTYLGRPWKEYSRTV+MKTY+DSLVDP GWLEWDG+FALNTLYY
Sbjct: 460 SRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYY 519

Query: 775 GEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           GEY NIGP S  SQRV WKGYHVI+N TEASEFTVQNFIAG+SWLPDTEVPFTPGL
Sbjct: 520 GEYNNIGPGSPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

[Close]
BLAST of Lsi05G013210 vs. ExPASy TrEMBL
Match: A0A1S3AYJ9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 7.3e-262
Identity = 454/525 (86.48%), Postives = 486/525 (92.57%), Query Frame = 0 

Query: 304 LSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATPK 363
           LSME  SL  VFLFLHFFLLSS+ FSKP +NG IDWWCKKTTYPETCKYFFNHG+K+ PK
Sbjct: 4   LSME-TSLVGVFLFLHFFLLSSSAFSKPASNG-IDWWCKKTTYPETCKYFFNHGTKSPPK 63

Query: 364 DMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYS 423
           DM +FKKMVIQFAME AL+AES+TK VGS CRNGKEKAAW DCLKLYQNTI QLNQTL S
Sbjct: 64  DMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDS 123

Query: 424 STTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNAS 483
           ST  T+FDIQTWLSTALTNLETCRTGFAELNV DYI P+IMS+NVTKLISNSLAINN ++
Sbjct: 124 STKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASA 183

Query: 484 SGVMGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGN 543
           +   GK TYKKGFPSW+S GDRRLLQS+S KVD+VVA+DGSGNY TVG A+EEA KRK +
Sbjct: 184 AVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTS 243

Query: 544 GRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGE 603
           GRFVI+VKRGVY EN+EIGSKMKNIMLIGDGMR TFITGNRSVGGGSTTFNSATVAVTGE
Sbjct: 244 GRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGE 303

Query: 604 GFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 663
           GFIA+GITFQNTAGPENHQAVALRSG+DLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG
Sbjct: 304 GFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 363

Query: 664 TVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELKI 723
           TVDFIFGNAAVVLQNCM+YARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATD+LK 
Sbjct: 364 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKP 423

Query: 724 VENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYYGEYKNIGPASS 783
           VE+TVKTYLGRPWKEYSRTV+MKTY+DSLVDP GWLEWDG+FALNTLYYGEY NIGP S 
Sbjct: 424 VESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSP 483

Query: 784 TSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
            SQRV WKGYHVISN+TEASEFTVQNFIAG+SWLP+TEVPFTPGL
Sbjct: 484 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]
BLAST of Lsi05G013210 vs. ExPASy TrEMBL
Match: A0A4Y1R295 (Pectinesterase OS=Prunus dulcis OX=3755 GN=Prudu_007577 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 3.2e-257
Identity = 510/983 (51.88%), Postives = 625/983 (63.58%), Query Frame = 0 

Query: 39   WCKKTTYPETCKYFFNHG-SKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGK 98
            WC+KT +PE C Y  +H   +  PK  +EF+KM++Q A+E ALNA+SY    G  C+N +
Sbjct: 35   WCQKTPHPEPCNYCMSHSRHRLVPKHTSEFRKMMVQVALERALNAKSYASQFGQNCQNNQ 94

Query: 99   EKAAWTDCLKLYQNTIFQLNQTLYSSTT---CTDFDIQTWLSTALTNLETCRTGFAELNV 158
            +KAAW DCLKL+++T+ QLN TL    T   C++FD QTWLS ALTN+ TC+ G  ELNV
Sbjct: 95   QKAAWADCLKLFEDTVHQLNITLEGLGTKRNCSNFDAQTWLSAALTNIHTCQVGSMELNV 154

Query: 159  SDYILPMIMS-NNVTKLISNSLAINNNASSGVMG-KETY-KKGFPSWVSSGDRRLLQSSS 218
            SD+I P+  S NNV++LISN LAI     S ++G +E Y  + +P+WVS  +RRLLQ+S 
Sbjct: 155  SDFIAPIYTSNNNVSELISNGLAI----GSQLLGTEENYADEEYPNWVSKHERRLLQASK 214

Query: 219  IKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIG 278
            IK ++VVA+DGSG++RTV  A++ A KRK   RF+I VK+GVY EN+E+ +   NIMLIG
Sbjct: 215  IKANLVVAKDGSGHFRTVQAAIDAAAKRKITSRFIIYVKKGVYRENIEVSNTNNNIMLIG 274

Query: 279  DGMRLTFITGNRSVGGGSTTFNSAT---------------------LNQEIISL------ 338
              MR T IT +RSV GGSTT+NSAT                     L  + ++L      
Sbjct: 275  ASMRYTIITASRSVNGGSTTYNSATAGIDGPGFIARGITFSNTAGPLKGQAVALRSASDL 334

Query: 339  ------------------------------------------------------------ 398
                                                                        
Sbjct: 335  SVFYRCAFQGYQDTLMVHSQRQFYRECYIYGTIDFIFGNAAAVFQSCIIYVRRPLKGQVN 394

Query: 399  ---------------------------------SMEKLSLGR--------VFL------- 458
                                             S  K  LGR        VF+       
Sbjct: 395  VITAQARNDPYQNTAISIHNSQVRAAADLKPVVSAFKTYLGRPWMQYSRVVFMKCYLDSL 454

Query: 459  -----------------------FLHFFLLSSTTF----------------SKPTTNGLI 518
                                   + +F   SST +                SK T + LI
Sbjct: 455  VNPVGWLEWQRSNFALSTLYYAEYKNFGPASSTRYRVKWPGFHVITSANVASKFTVSSLI 514

Query: 519  -----------DWWCKKTTYPETCKYFFNHGSK-ATPKDMAEFKKMVIQFAMETALNAES 578
                        +   KT YPETCKY   H  K + P  M++FKK+ +Q  M+ AL A+S
Sbjct: 515  TGRSWLPATGVPYTAGKTPYPETCKYTLTHAQKYSVPTRMSDFKKLAVQVTMQQALKAQS 574

Query: 579  YTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLET 638
            + K +G  CRN  EKAAW DCL LYQ+TI  LNQT+  +T CTD+D QTWLSTALTNL+T
Sbjct: 575  HNKWLGPKCRNKIEKAAWADCLSLYQDTIMLLNQTIDPATKCTDYDAQTWLSTALTNLDT 634

Query: 639  CRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDR 698
            CR GF EL VSD++LP +MSNNV+KLISN+L+I N ++   +    YK+GFP+WVS GDR
Sbjct: 635  CRAGFVELGVSDFVLP-LMSNNVSKLISNTLSIGNGSNVPAV-TNRYKEGFPTWVSPGDR 694

Query: 699  RLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKM 758
            +LLQSS    DVVVA+DGSGNY+T+ E +  A KR G+ RFVI VKRGVY+EN+EI  K+
Sbjct: 695  KLLQSSP-AADVVVAQDGSGNYKTIKEGLAAAEKRSGSNRFVIHVKRGVYKENLEI--KL 754

Query: 759  KNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVA 818
            KNIML+GDG+R T ITG+RSV GGSTTFNSATVAVTG+GFIA+GITF+NTAGPENHQAVA
Sbjct: 755  KNIMLLGDGLRYTIITGSRSVVGGSTTFNSATVAVTGDGFIARGITFRNTAGPENHQAVA 814

Query: 819  LRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARK 829
            LRSG+DLSVFYRC FEGYQDTLYVHSQRQFYKECY+YGTVDFIFGNAAVVLQNCM+YAR+
Sbjct: 815  LRSGADLSVFYRCGFEGYQDTLYVHSQRQFYKECYVYGTVDFIFGNAAVVLQNCMIYARR 874

[Close]
BLAST of Lsi05G013210 vs. NCBI nr
Match: KAF9668232.1 (hypothetical protein SADUNF_Sadunf15G0107700 [Salix dunnii])

HSP 1 Score: 926.4 bits (2393), Expect = 1.7e-265
Identity = 504/977 (51.59%), Postives = 628/977 (64.28%), Query Frame = 0 

Query: 36   IDWWCKKTTYPETCKYFFNHGSK-ATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCR 95
            ID+WC KT  PE CKYF     K   PK  ++F+KM I+ AM+ AL+A+++ K +G  CR
Sbjct: 519  IDYWCSKTPNPEPCKYFMKQNPKHFLPKQKSDFRKMAIELAMQRALHAQNHNKWLGPKCR 578

Query: 96   NGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNV 155
            N KEKAAW DCL+LY++TI +LN+T+ S T CT+FD QTWLSTALTNLETC+ GF +  V
Sbjct: 579  NEKEKAAWADCLRLYEDTIVELNRTIVSKTKCTEFDSQTWLSTALTNLETCKAGFRDFGV 638

Query: 156  SDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSSSIKV 215
            SD++LP +MSNNV+KLI N+LA+ +NASS +   +TYK  FP WV +GDR+LLQ++S   
Sbjct: 639  SDFVLP-LMSNNVSKLIRNTLALKDNASSTL--PQTYKDSFPGWVKAGDRKLLQTTSPTP 698

Query: 216  DVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGM 275
            ++VVA+DGSGNY T+  A++ A KR G+ RFVI++K GVY EN++IG  +KNIML+GDG+
Sbjct: 699  NLVVAQDGSGNYLTIKAALDAAAKRSGSRRFVIRIKSGVYRENLDIGKNLKNIMLVGDGL 758

Query: 276  RLTFITGNRSVGGGSTTFNSATL------------------------------------- 335
            R T ITG+RSVGGG TTFNSAT+                                     
Sbjct: 759  RYTIITGSRSVGGGFTTFNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVF 818

Query: 336  ------------------------------------------------------------ 395
                                                                        
Sbjct: 819  YRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVFQNCMIYARRPMAQQKNVVT 878

Query: 396  ----------------NQEIIS-------LSMEKLSLGRVF------LFLHFFL------ 455
                            N  +++       LS  K  LGR +      +FL  +L      
Sbjct: 879  AQGRTDPNQNTGISIHNSRVMASSDLRPVLSSFKTFLGRPWKEYSRTVFLQTYLDSLVDP 938

Query: 456  -----------------------------------------LSSTTFSKPTTNGLI---- 515
                                                      SST  S+ T    I    
Sbjct: 939  AGWLEWDGNFALNTLYYGEYRNFGPASSTGGRVKWGGYRVITSSTEASRFTVANFIAGRS 998

Query: 516  -----DWWCKKTTYPETCKYFFNHGSK-ATPKDMAEFKKMVIQFAMETALNAESYTKDVG 575
                 D+WC KT  PE CKYF     K   PK  ++F+KM I+ AM+ AL+A+++ K +G
Sbjct: 999  WLPATDYWCSKTPNPEPCKYFMKQNPKHFLPKQKSDFRKMSIELAMQRALHAQNHNKWLG 1058

Query: 576  SMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFA 635
              CRN KEKAAW DCL+LY++TI  LN+T+ S   CT+FD QTWLST+LTNLETC+ GF 
Sbjct: 1059 PKCRNEKEKAAWADCLRLYEDTIVDLNRTIDSKIKCTEFDAQTWLSTSLTNLETCKAGFK 1118

Query: 636  ELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSS 695
            +L VSD++LP +MSNNV+ LI N+LA+ +NASS +   +TYK  FP WV +GDR+LLQ+S
Sbjct: 1119 DLGVSDFVLP-LMSNNVSNLIRNTLALKDNASSTL--PQTYKDSFPGWVKAGDRKLLQTS 1178

Query: 696  SIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLI 755
            S   ++VVA+DGSGNYRT+  A++ A +R  +GRFVI++K G Y EN++IG  +KNIML+
Sbjct: 1179 SPTPNLVVAQDGSGNYRTIKAALDAAAQRSDSGRFVIRIKSGAYSENLDIGKNLKNIMLV 1238

Query: 756  GDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSD 815
            GDG+R T ITG+RSV GG TTFNSATVAVTG+GFIA+GITF+NTAGP+N+QAVALRSGSD
Sbjct: 1239 GDGLRNTIITGSRSVSGGFTTFNSATVAVTGDGFIARGITFRNTAGPQNNQAVALRSGSD 1298

Query: 816  LSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQK 829
            LSVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCM+YAR+PM  QK
Sbjct: 1299 LSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMAQQK 1358

[Close]
BLAST of Lsi05G013210 vs. NCBI nr
Match: GAY53646.1 (hypothetical protein CUMW_150710 [Citrus unshiu])

HSP 1 Score: 926.4 bits (2393), Expect = 1.7e-265
Identity = 523/1056 (49.53%), Postives = 641/1056 (60.70%), Query Frame = 0 

Query: 3    LSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHG-SKATP 62
            ++ E +SL  +F+ L      + +     TN  I WWC KT +PE CKYF +    + T 
Sbjct: 1    MAKENISLLIIFISLSSIFYPALSRRPSATN--ITWWCSKTPHPEPCKYFLSRSHHRYTF 60

Query: 63   KDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLY 122
            K  ++F+K+++Q A+E A  A     + G+ C N  +KAAW+DC+KLY NT+ QLN+TL 
Sbjct: 61   KHRSDFRKIIVQVAIEQAHKAHDQVMEFGTNCENKHQKAAWSDCVKLYANTVLQLNRTLQ 120

Query: 123  ---SSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAIN 182
               S  +CTDFD QTWLSTALTN+ TC++G+ ELNVSD+I P +MSNN+++LISNSLA+N
Sbjct: 121  GLKSDRSCTDFDAQTWLSTALTNIRTCQSGYMELNVSDFITP-VMSNNLSQLISNSLAVN 180

Query: 183  NNASSGVMGKE---TYKKGFPSWVSSGDRRLLQSSSI--KVDVVVARDGSGNYRTVGEAV 242
                 GV+ K    TY  GFPSW+S  +R LL+SSS+  + ++VVA DGSGNYRT+  A+
Sbjct: 181  -----GVLLKRENVTYTNGFPSWLSGHERNLLESSSLEARANLVVATDGSGNYRTIQAAI 240

Query: 243  EEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFN 302
              A +R+G+GRF+I VKRGVY EN+E+G    NIML+G+GMR T IT  +SVG GSTT++
Sbjct: 241  NAAARRRGSGRFIIHVKRGVYRENIEVGLNNNNIMLVGEGMRNTIITSGQSVGSGSTTYS 300

Query: 303  SATL----------------------NQEIISLSMEKLS--------------------- 362
            SAT                        Q +   S   LS                     
Sbjct: 301  SATAGIDGLHFMGRDITFQNTAGPLKGQAVALRSASDLSVFYRCAFQGYQDTLMVHSQRQ 360

Query: 363  ------------------------------------------------------------ 422
                                                                        
Sbjct: 361  FYKKCYIYGTIDFIFGNAAVVFQNCIIFVRKPLKGQANVITAQGRNDPFQNTAISIHSSR 420

Query: 423  -----------------LGR---------------------------------------- 482
                             LGR                                        
Sbjct: 421  VLPANDLKPVVRNFKTYLGRPWQQYSRTVILKTYIDGFVSPLGWSTWSPGNNFALDTLFY 480

Query: 483  -----------------------------------------------------------V 542
                                                                        
Sbjct: 481  GEYENYGPGSSTRHRVKWRGFHVITSPKVASQFTVGSLIAGRSWLPATASALMAMKLSVF 540

Query: 543  FLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSK--ATPKDMAEFKKMV 602
             LF+      +   S   +NG +D+WC KT +PE CKYF    SK  A PK  +EF++M 
Sbjct: 541  LLFISLISFFAPALSSVASNG-VDYWCSKTPHPEPCKYFMQQNSKHFAVPKQKSEFRRMA 600

Query: 603  IQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDI 662
            +  A++ AL A+++ K +GS CRN KEK AW DCLKLYQ+TI QLN TL S+T CTDFD 
Sbjct: 601  MSLALDRALTAQNHNKWLGSKCRNHKEKVAWADCLKLYQDTINQLNHTLDSNTKCTDFDA 660

Query: 663  QTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKETY 722
            QTWLSTALTNLETCR GF EL V DY+LP +MSNNVTKLISN+LA+ N ++      ETY
Sbjct: 661  QTWLSTALTNLETCRAGFVELGVPDYVLP-LMSNNVTKLISNTLALRNASTV----PETY 720

Query: 723  KKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKR 782
            K GFPSWV  GDR+LLQ+S ++ ++VVA+DGSGNYRT+  A++ A KR G+GRFVI VKR
Sbjct: 721  KGGFPSWVKPGDRKLLQTSPVRPNLVVAQDGSGNYRTIKAALDAAAKRTGSGRFVIHVKR 780

Query: 783  GVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITF 829
            GVY+EN+EIG+KMKNIML+GDG+R T ITG+RSVGGG TTFNSATVAVTG+GFIA+GITF
Sbjct: 781  GVYKENLEIGNKMKNIMLVGDGLRYTIITGSRSVGGGFTTFNSATVAVTGDGFIARGITF 840

[Close]
BLAST of Lsi05G013210 vs. NCBI nr
Match: KAA0067674.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 917.9 bits (2371), Expect = 6.2e-263
Identity = 459/526 (87.26%), Postives = 488/526 (92.78%), Query Frame = 0 

Query: 304 LSMEKLSLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATPK 363
           LSME  SL  VFLFLHFFLLSS+ FSKP +NG IDWWCKKTTYPETCKYFFNHG+K+ PK
Sbjct: 4   LSME-TSLVGVFLFLHFFLLSSSAFSKPASNG-IDWWCKKTTYPETCKYFFNHGTKSPPK 63

Query: 364 DMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYS 423
           DM +FKKMVIQFAME AL+AES+TK VGS CRNGKEKAAW DCLKLYQNTI QLNQTL S
Sbjct: 64  DMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDS 123

Query: 424 STTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNAS 483
           ST CT+FDIQTWLSTALTNLETCRTGFAELNV DYI P+IMS+NVTKLISNSLAI NNAS
Sbjct: 124 STKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAI-NNAS 183

Query: 484 SGV-MGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKG 543
           +GV  GK TYKKGFPSW+S GDRRLLQS S KVD+VVA+DGSGNY TVG A+EEA KRK 
Sbjct: 184 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 243

Query: 544 NGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTG 603
           +GRFVI+VKRGVY EN+EIGSKMKNIMLIGDGMR TFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 244 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 303

Query: 604 EGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 663
           EGFIA+GITFQNTAGPENHQAVALRSG+DLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 304 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 363

Query: 664 GTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELK 723
           GTVDFIFGNAAVVLQNCM+YARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATD+LK
Sbjct: 364 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 423

Query: 724 IVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYYGEYKNIGPAS 783
            VE+TVKTYLGRPWKEYSRTV+MKTY+DSLVDP GWLEWDG+FALNTLYYGEY NIGP S
Sbjct: 424 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 483

Query: 784 STSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
             SQRV WKGYHVISN+TEASEFTVQNFIAG+SWLP+TEVPFTPGL
Sbjct: 484 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]
BLAST of Lsi05G013210 vs. NCBI nr
Match: XP_038882414.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 916.4 bits (2367), Expect = 1.8e-262
Identity = 457/519 (88.05%), Postives = 485/519 (93.45%), Query Frame = 0 

Query: 310 SLGRVFLFLHFFLLSSTTFSKPTTNGLIDWWCKKTTYPETCKYFFNHGSKATPKDMAEFK 369
           SLG VF FLHFFLLSS  FSK  +NG IDWWC+KTTYPETCKYFFN GSKA PKDM EFK
Sbjct: 4   SLG-VFFFLHFFLLSSAAFSKHASNG-IDWWCRKTTYPETCKYFFNQGSKAAPKDMTEFK 63

Query: 370 KMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTLYSSTTCTD 429
           KMVIQFAME AL AES+TK VGS CRNGKEKAAW DCLKLYQNTIFQLNQTL S+T CT+
Sbjct: 64  KMVIQFAMERALGAESHTKGVGSKCRNGKEKAAWADCLKLYQNTIFQLNQTLDSATKCTE 123

Query: 430 FDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGK 489
           FDIQTWLSTALTNLETCRTGFAELNVSDYIL +IMSNNVTKLISNSLAI NNAS+G +GK
Sbjct: 124 FDIQTWLSTALTNLETCRTGFAELNVSDYILQLIMSNNVTKLISNSLAI-NNASAG-LGK 183

Query: 490 ETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIK 549
           ETYKKGFPSW+S GDRRLLQSSS KVD+VVA+DGSGN+ TVG A+EEA KRK NGRFVI+
Sbjct: 184 ETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNHTTVGAALEEAAKRKTNGRFVIQ 243

Query: 550 VKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQG 609
           VKRGVY+EN+EIGSKMKNIMLIGDGMR TFITGNRSVGGGSTTFNSATVAVTGEGFIA+G
Sbjct: 244 VKRGVYQENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARG 303

Query: 610 ITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 669
           ITF+NTAGPENHQAVALRSG+DLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 304 ITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 363

Query: 670 GNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELKIVENTVK 729
           GNAAVVLQNCM+YAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATD+LK VE+TVK
Sbjct: 364 GNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVK 423

Query: 730 TYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYYGEYKNIGPASSTSQRVN 789
           TYLGRPWKEYSRTV+MKTYLDSLVDP GWLEWDG+FAL+TLYYGEY NIGP S TSQRVN
Sbjct: 424 TYLGRPWKEYSRTVFMKTYLDSLVDPTGWLEWDGDFALDTLYYGEYNNIGPRSPTSQRVN 483

Query: 790 WKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           WKGY VI N+TEASEFTVQNFIAG+SWLPDTEVPFTPGL
Sbjct: 484 WKGYRVIRNSTEASEFTVQNFIAGQSWLPDTEVPFTPGL 518

[Close]
BLAST of Lsi05G013210 vs. NCBI nr
Match: XP_004148139.2 (pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [Cucumis sativus])

HSP 1 Score: 914.4 bits (2362), Expect = 6.8e-262
Identity = 461/536 (86.01%), Postives = 495/536 (92.35%), Query Frame = 0 

Query: 295 ATLNQEIISLSMEKLSLGRVFLFLHFFLLSSTT-FSKPTTNGLIDWWCKKTTYPETCKYF 354
           A+ N E+  LSM   S G VF+FLHFFLLSS++ FSKP +NG IDWWCKKTTYPETCKYF
Sbjct: 40  ASSNIEMAGLSM-GTSFG-VFIFLHFFLLSSSSAFSKPASNG-IDWWCKKTTYPETCKYF 99

Query: 355 FNHGSKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNT 414
           FNHG+K+ PKDM +FKKMVIQFAME AL+AES+TK VGS CRNGKEKAAW DCLKLYQNT
Sbjct: 100 FNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNT 159

Query: 415 IFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLIS 474
           I QLNQTL SST  T+FDIQTWLSTALTNLETCRTGFAELNVSDYILP+IMS+NVT+LIS
Sbjct: 160 ILQLNQTLDSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELIS 219

Query: 475 NSLAINNNASSGV-MGKETYKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGE 534
           NSLAI NNAS+GV  GKETYKKGFPSW+S GDRRLLQSSS KVD+VVA+DGSGNY TVG 
Sbjct: 220 NSLAI-NNASAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGA 279

Query: 535 AVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTT 594
           A+EEA KRK +GRFVI+VKRGVY EN+EIGSKMKNIMLIGDGMR TFITGNRSVGGGSTT
Sbjct: 280 ALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTT 339

Query: 595 FNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQ 654
           FNSATVAVTGEGFIA+GITF+NTAGPENHQAVALRSG+DLSVFYRCAFEGYQDTLYVHSQ
Sbjct: 340 FNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQ 399

Query: 655 RQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHN 714
           RQFYKECYIYGTVDFIFGNAAVVLQNCM+YARKPMNGQKNAVTAQGRTDPNQNTGISIHN
Sbjct: 400 RQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHN 459

Query: 715 SRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEWDGEFALNTLYY 774
           SRVMATD+LK VE+TVKTYLGRPWKEYSRTV+MKTY+DSLVDP GWLEWDG+FALNTLYY
Sbjct: 460 SRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYY 519

Query: 775 GEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           GEY NIGP S  SQRV WKGYHVI+N TEASEFTVQNFIAG+SWLPDTEVPFTPGL
Sbjct: 520 GEYNNIGPGSPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

[Close]
BLAST of Lsi05G013210 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 582.4 bits (1500), Expect = 5.7e-166
Identity = 290/490 (59.18%), Postives = 374/490 (76.33%), Query Frame = 0 

Query: 340 WCKKTTYPETCKYFFNHGSKATP-KDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGK 399
           WC +T  P+ C+YF  H S   P K  +EF K+ ++  ++ A+ A+++   +G  CR+ +
Sbjct: 33  WCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTR 92

Query: 400 EKAAWTDCLKLYQNTIFQLNQTLYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDY 459
           EKAAW DC+KLY  T+ ++N+T+  +  C+  D QTWLSTALTNL+TCR GF EL V+D 
Sbjct: 93  EKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDI 152

Query: 460 ILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSSSIKVDVV 519
           +LP +MSNNV+ L+ N+LAIN    +    +   K GFPSWV  GDR+LLQSS+ K + V
Sbjct: 153 VLP-LMSNNVSNLLCNTLAINKVPFNYTPPE---KDGFPSWVKPGDRKLLQSSTPKDNAV 212

Query: 520 VARDGSGNYRTVGEAVEEAGKRKGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLT 579
           VA+DGSGN++T+ EA++ A    G+GRFVI VK+GVY EN+EI  + KN+ML GDG+  T
Sbjct: 213 VAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKT 272

Query: 580 FITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRC 639
            ITG++SVGGG+TTFNSATVA  G+GFIA+GITF+NTAG  N QAVALRSGSDLSVFY+C
Sbjct: 273 IITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQC 332

Query: 640 AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQG 699
           +FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VLQNC ++AR+P   + N +TAQG
Sbjct: 333 SFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQG 392

Query: 700 RTDPNQNTGISIHNSRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGW 759
           R+DPNQNTGI IHNSRV A  +L+ V  + KTYLGRPW++YSRTV+MKT LDSL+DP GW
Sbjct: 393 RSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGW 452

Query: 760 LEWDGEFALNTLYYGEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLP 819
           LEWDG FAL TL+Y E++N GP +STS RV W G+ V+ +A+EAS+FTV  F+AG SW+P
Sbjct: 453 LEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIP 511

Query: 820 DTEVPFTPGL 829
            + VPFT GL
Sbjct: 513 -SSVPFTSGL 511

[Close]
BLAST of Lsi05G013210 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 569.7 bits (1467), Expect = 3.8e-162
Identity = 294/543 (54.14%), Postives = 389/543 (71.64%), Query Frame = 0 

Query: 302 ISLSMEKLSLGRVFLFLHFFLLSSTTFSKPTT---NGLIDWWCKKTTYPETCK-YFFNHG 361
           +++ M+KLS+    LFLH  LL        TT       D WC KT YP+ CK YF NH 
Sbjct: 1   MNMMMQKLSI----LFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHN 60

Query: 362 SKATPKDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQL 421
               P  ++EF+ M+++ AM+ A++A +   + G  C + K++A   DC+ LY +TI QL
Sbjct: 61  GFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQL 120

Query: 422 NQTLY-------SSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTK 481
           N+TL+       ++ +CTDFD QTWLSTALTN ETCR G ++LNV+D+I P++ +  ++ 
Sbjct: 121 NRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISH 180

Query: 482 LISNSLAINNN-ASSGVMGKET-YKKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYR 541
           LISN LA+N    ++G  G  T  +KGFP+W+S  D+RLL+  +++ ++VVA+DGSG++ 
Sbjct: 181 LISNCLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFN 240

Query: 542 TVGEAVEEAGKRK-GNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVG 601
           TV  A++ AG+RK  +GRFVI VKRG+Y+EN+ +     +IML+GDGMR T ITG RSV 
Sbjct: 241 TVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQ 300

Query: 602 GGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTL 661
           GG TT+NSAT  + G  FIA+GITF+NTAGP   QAVALRS SDLS+FY+C+ EGYQDTL
Sbjct: 301 GGYTTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTL 360

Query: 662 YVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTG 721
            VHSQRQFY+ECYIYGTVDFIFGNAA V QNC++  R+P+ GQ N +TAQGR DP QNTG
Sbjct: 361 MVHSQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTG 420

Query: 722 ISIHNSRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEW--DGEF 781
           ISIHNSR++   +LK V  TVKTY+GRPW ++SRTV ++TYLD++V P+GW  W     F
Sbjct: 421 ISIHNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVF 480

Query: 782 ALNTLYYGEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFT 829
            L+TL+Y EYKN GPASST  RV+WKG+HV+  A++AS FTV  FIAG +WLP T +PFT
Sbjct: 481 GLDTLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFT 536

[Close]
BLAST of Lsi05G013210 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 556.2 bits (1432), Expect = 4.4e-158
Identity = 290/530 (54.72%), Postives = 370/530 (69.81%), Query Frame = 0 

Query: 315 FLFLHFFLLSSTTFSKPTT-----NGLIDWWCKKTTYPETCK-YFFNHGSKATPKDMAEF 374
           FL LH  L+        TT     +  ID WC KT YP  CK YF  H     P  ++EF
Sbjct: 11  FLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70

Query: 375 KKMVIQFAMETALNAESYTKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTL--YSSTT 434
           + ++++ AM+ A++A     +    C + K++A   DC+ LY +T+ QLN+TL   SS T
Sbjct: 71  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130

Query: 435 ---CTDFDIQTWLSTALTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNAS 494
              CTDFD QTWLSTALTN ETCR G ++LNVSD+  P++ +  ++ LISN LA+N    
Sbjct: 131 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALL 190

Query: 495 SGVMGKETY--KKGFPSWVSSGDRRLLQSSSIKVDVVVARDGSGNYRTVGEAVEEAGKRK 554
           +      T    KGFP+WVS  +RRLLQ  S++ ++VVA+DGSG+++TV  A++ AG+RK
Sbjct: 191 TAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRK 250

Query: 555 -GNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAV 614
             +GRFVI VKRG+Y+EN+ +     NIML+GDGMR T ITG RSV GG TT++SAT  +
Sbjct: 251 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

Query: 615 TGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECY 674
            G  FIA+GI FQNTAGP   QAVALRS SDLS+FYRC+ EGYQDTL VHSQRQFY+ECY
Sbjct: 311 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 370

Query: 675 IYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDE 734
           IYGTVDFIFGNAAVV QNC++  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   +
Sbjct: 371 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 430

Query: 735 LKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEW--DGEFALNTLYYGEYKNI 794
           LK V  +VKTY+GRPW  YSRTV +KTY+DS+V P+GW  W     + L+TL+Y EYKNI
Sbjct: 431 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 490

Query: 795 GPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           GPASST  RV WKG+HV+S A++AS F+V  FIAG +WLP + +PFT  L
Sbjct: 491 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

[Close]
BLAST of Lsi05G013210 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 538.9 bits (1387), Expect = 7.2e-153
Identity = 273/521 (52.40%), Postives = 359/521 (68.91%), Query Frame = 0 

Query: 332 TTNGLIDWWCKKTTYPETCKYFFNHGSK-----ATPKDMAEFKKMVIQFAMETALNAESY 391
           + +G + WWC KT + ETC Y+F   S+       P+  +EF +M+++ A++ A+   S 
Sbjct: 79  SVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQ 138

Query: 392 TKDVGSMCRNGKEKAAWTDCLKLYQNTIFQLNQTL-------YSSTTCTDFDIQTWLSTA 451
           T   G  C N + KAAW+DC+ L+QNT+ QLN+TL        S   CTDFD QTWLSTA
Sbjct: 139 TVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTA 198

Query: 452 LTNLETCRTGFAELNVSDYILPMIMSNNVTKLISNSLAINNNASSGVMGKE-------TY 511
            TN+ETCR+G  +LNVSD+++P+I + N++ LI N LA+N     GV+ K+        +
Sbjct: 199 QTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVN-----GVLMKQHDHTTTANH 258

Query: 512 KKGFPSWVSSGDRRLLQSSSI---KVDVVVARDGSGNYRTVGEAVEEAGKRKGNGRFVIK 571
           K+ FPSWVS  +RRLL S+S+      +VVA+D SG++R++  A+  A +R+   RFVI 
Sbjct: 259 KEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIY 318

Query: 572 VKRGVYEENVEIGSKMKNIMLIGDGMRLTFITGNRSVGGGSTTFNSATVAVTGEGFIAQG 631
           VK+GVY EN+++G+   NIML+GDG R T IT  RSV  G TT+NSAT    G+ F+A+ 
Sbjct: 319 VKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKD 378

Query: 632 ITFQNTAGPENHQAVALRSGSDLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 691
           +TF NTAGP   QAVA+RS SDLSVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIF
Sbjct: 379 MTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIF 438

Query: 692 GNAAVVLQNCMLYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDELKIVENTVK 751
           GNAAVV QNCM+  R+P++GQ N +TAQGR DP QNTGI+IH+SR++A  +LK V    K
Sbjct: 439 GNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYK 498

Query: 752 TYLGRPWKEYSRTVYMKTYLDSLVDPMGWLEW--DGEFALNTLYYGEYKNIGPASSTSQR 811
           TYLGRPW+ YSR   MKTY+D+ + P+GW  W     FALNT++YGEYKN GP SST  R
Sbjct: 499 TYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWR 558

Query: 812 VNWKGYHVISNATEASEFTVQNFIAGESWLPDTEVPFTPGL 829
           V WKG+H I++   AS FTV + IAG SWLP T VPF  GL
Sbjct: 559 VRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]
BLAST of Lsi05G013210 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 452.2 bits (1162), Expect = 8.9e-127
Identity = 249/491 (50.71%), Postives = 326/491 (66.40%), Query Frame = 0 

Query: 341 CKKTTYPETCKYFFNHGSKATP-KDMAEFKKMVIQFAMETALNAESYTKDVGSMCRNGKE 400
           C +T +P  CK       K  P +   +F ++ ++  ++ A+ A+S   D   +      
Sbjct: 8   CDETPHPGECKTLL---IKHKPIRSTTQFLQVSVERTLDGAVKAKS---DTYFLEPQFGS 67

Query: 401 KAAWTDCLKLYQNTIFQLNQT-LYSSTTCTDFDIQTWLSTALTNLETCRTGFAELNVSDY 460
           K AW +C+ LY+ TI +LN++ L     C+  D+Q WLSTALTNL+TC+   +EL VS +
Sbjct: 68  KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVSSH 127

Query: 461 ILPMIMSNNVTKLISNSLAINNNASSGVMGKETYKKGFPSWVSSGDRRLLQSSSIKVDVV 520
            L      ++T  + N+LAIN         K   + G    +S    + L S   KVDVV
Sbjct: 128 SL-----ESITIDVINTLAIN---------KRMEQNGKEFGISKITMKTL-SIGEKVDVV 187

Query: 521 VARDGSGNYRTVGEAVEEAGKR-KGNGRFVIKVKRGVYEENVEIGSKMKNIMLIGDGMRL 580
           VA+DGSG+Y+T+ EAV  AG+R KG+ R+VI VK+GVYEE V +G K  NIM+ GDG+  
Sbjct: 188 VAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIGK 247

Query: 581 TFITGNRSVGGGSTTFNSATVAVTGEGFIAQGITFQNTAGPENHQAVALRSGSDLSVFYR 640
           T ITG++S G G +T+ SAT    G+GF+ + IT +NTAGPENHQAVALRS SD+SVFYR
Sbjct: 248 TIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSDMSVFYR 307

Query: 641 CAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMLYARKPMNGQKNAVTAQ 700
           C+ EGYQDTLYVHS RQF++EC IYGTVDFIFGNAA VLQNC ++AR P NG  N +TAQ
Sbjct: 308 CSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNG-VNTITAQ 367

Query: 701 GRTDPNQNTGISIHNSRVMATDELKIVENTVKTYLGRPWKEYSRTVYMKTYLDSLVDPMG 760
            R +PNQ TGI IHNS V     +++    VKTYLGRPW+ Y+RTV + TYLD+L++P G
Sbjct: 368 SRFNPNQTTGIVIHNSVVKGAPGVQL--GGVKTYLGRPWRSYARTVVIGTYLDTLIEPNG 427

Query: 761 WLEWDGEFALNTLYYGEYKNIGPASSTSQRVNWKGYHVISNATEASEFTVQNFIAGESWL 820
           W++WD   AL+TLYYGEY+N GP S T  RV+W G+HVIS+  EA EFT+  FI   SWL
Sbjct: 428 WIDWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSASWL 474

Query: 821 PDTEVPFTPGL 829
           P T+VPFT  L
Sbjct: 488 PPTKVPFTINL 474

The following BLAST results are available for this feature:
BLAST of Lsi05G013210 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O048871.6e-17360.55Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221498.0e-16559.18Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN55.4e-16154.14Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN46.2e-15754.72Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY31.0e-15152.40Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
BLAST of Lsi05G013210 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A2H5PMQ88.4e-26649.53Pectinesterase OS=Citrus unshiu OX=55188 GN=CUMW_150710 PE=3 SV=1[more]
A0A5D3DJL73.0e-26387.26Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440... [more]
A0A0A0L9G43.3e-26286.01Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1[more]
A0A1S3AYJ97.3e-26286.48Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1[more]
A0A4Y1R2953.2e-25751.88Pectinesterase OS=Prunus dulcis OX=3755 GN=Prudu_007577 PE=3 SV=1[more]
BLAST of Lsi05G013210 vs. NCBI nr
Match NameE-valueIdentityDescription
KAF9668232.11.7e-26551.59hypothetical protein SADUNF_Sadunf15G0107700 [Salix dunnii][more]
GAY53646.11.7e-26549.53hypothetical protein CUMW_150710 [Citrus unshiu][more]
KAA0067674.16.2e-26387.26pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [... [more]
XP_038882414.11.8e-26288.05pectinesterase-like [Benincasa hispida][more]
XP_004148139.26.8e-26286.01pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [... [more]
BLAST of Lsi05G013210 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45220.15.7e-16659.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.13.8e-16254.14Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.14.4e-15854.72Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.17.2e-15352.40Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G00190.18.9e-12750.71pectin methylesterase 38 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 332..478
e-value: 2.9E-29
score: 113.2
coord: 31..177
e-value: 2.9E-29
score: 113.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 13..180
e-value: 1.7E-26
score: 90.6
coord: 314..481
e-value: 1.7E-26
score: 90.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 35..177
e-value: 3.3E-26
score: 92.4
coord: 336..478
e-value: 3.3E-26
score: 92.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 332..482
e-value: 3.0E-29
score: 104.0
coord: 31..181
e-value: 3.0E-29
score: 104.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 34..180
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 335..481
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 510..828
e-value: 1.7E-123
score: 414.1
coord: 209..315
e-value: 5.5E-23
score: 83.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 516..813
e-value: 1.1E-145
score: 484.0
coord: 215..299
e-value: 8.6E-26
score: 90.3
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 313..827
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 12..297
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 12..297
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 313..827
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 339..484
e-value: 7.94835E-34
score: 125.243
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 38..183
e-value: 7.94835E-34
score: 125.243
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 661..670
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 513..827
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 212..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G013210.1Lsi05G013210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity