Lsi05G012810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G012810
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	gibberellin 20 oxidase 2-like
Location	chr05: 20574515 .. 20575923 (-)
RNA-Seq Expression	Lsi05G012810
Synteny	Lsi05G012810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTACAGGGGAATCAAAGGAGATGAAAAACGAGCTGCACCGTGTGGAATTTGGGTGCTCGGAGGAGTCAATGGTGCCGGAGAATTTCGTGTGGCCGGACGAGTTTAAAGCAAGGGAAGGGGCGCCGGAGCTGCATGTTCCACATATTGACTTGCAAAAGATCTTAAGTGGAGATGAAAGTGATATTGAAGAGGCAACCAGGCTGGTAGATGAAGCTTGTAGAAAGCATGGATTCTTTGTGTTGATCAACCATGGAGTGGATATGGAATTGATGAAAAAAGTTCATCAGTGTATGGATGAGTTCTTTACATTGCCTTTTGATGTCAAACAGAAATCTCAAAGGAAATTCGGTGAAAATTATGGGTATGCTAATAGCTTCATTGGAAGATTCTCCAATAATCTTCCATGGAAGGAAACTTTTTCCCTTCCTTGTGTTGCTCATCACAATTCAACCTCGGTTCAAGATTATGTTTCTCAATATTATGGCTCAGAATTCCCCCACCACGGGTAAGTACGATTTAAACTTCATTATTGTTGAAATGTCTTGAATCTGTACTCTAGATGCTCTATCCTCTAATTTGATATTTTTCTCACTAATAATAAATTATTCTCTTTCTTTATTTCTCGATATAGCTAACCCGTGGTTAAGAATATTTCGTGGTTTATGTTGTTGTGTAGGAAGGTGTATCAGGAGTGTGGAAAGGCATTGAGTGATTTGAGTTTGAAGATAGTGGAGTTATTGGGGTTAAGCCTAGGGGTACCAAAAGAGAAGTTCAAGAAATTCTATGAGGACAATGATTCAATAATAAGGCTAAATTATTACCCACCATGCGAGAAGCCAGAGCTGACATTGGGAACTGGGCCTCACTGTGATCCAACCTCCATAACAATTCTTCACCAAGACCATGTCAGTGGCCTTCAAGTCTACGTGGATGATGAGTGGCACTCAATTCCTCCAACCAAGGATTCCTTTGTCATCAACATCGGTGACACTTTCATGGTAATTAATCTTTTTTTTTTTTTTTGGTTACAAAATTTTCAAACTATAATGAATTACTTTAGTGAAAAATTTCAATTTTCTTAATTAATAAGGCTCTAACAAATGGGGTTTACAAGAGCTGCTTCCACCGAGCTGTAGTGAATTGCAAGGAAGCAAGAAAATCAATGGCCTTTTTTCTATGTCCAGCGGCTGACAAAGTGGTGAGAGCACCGGAGGAAGTGGTGGAGAAGAATCCACCACGAAAGTTTCCAGATTACACATGGCCAATGCTGCTTGAACTTACCCAAAAATTTTACAGGGCTGATTCCAATACTTTTAAGGCCTTCATACCTTGGCTTGAAGAACAAAAGTTGGGTAATACTATTGCATCACCGGCTCTTTAAATTATCCAACCCTGCCCCTTTAAA

mRNA sequence

ATGAGGGAATCAAAGGAGATGAAAAACGAGCTGCACCGTGTGGAATTTGGGTGCTCGGAGGAGTCAATGGTGCCGGAGAATTTCGTGTGGCCGGACGAGTTTAAAGCAAGGGAAGGGGCGCCGGAGCTGCATGTTCCACATATTGACTTGCAAAAGATCTTAAGTGGAGATGAAAGTGATATTGAAGAGGCAACCAGGCTGGTAGATGAAGCTTGTAGAAAGCATGGATTCTTTGTGTTGATCAACCATGGAGTGGATATGGAATTGATGAAAAAAGTTCATCAGTGTATGGATGAGTTCTTTACATTGCCTTTTGATGTCAAACAGAAATCTCAAAGGAAATTCGGTGAAAATTATGGGTATGCTAATAGCTTCATTGGAAGATTCTCCAATAATCTTCCATGGAAGGAAACTTTTTCCCTTCCTTGTGTTGCTCATCACAATTCAACCTCGGTTCAAGATTATATAGTGGAGTTATTGGGGTTAAGCCTAGGGGTACCAAAAGAGAAGTTCAAGAAATTCTATGAGGACAATGATTCAATAATAAGGCTAAATTATTACCCACCATGCGAGAAGCCAGAGCTGACATTGGGAACTGGGCCTCACTGTGATCCAACCTCCATAACAATTCTTCACCAAGACCATGTCAGTGGCCTTCAAGTCTACGTGGATGATGAGTGGCACTCAATTCCTCCAACCAAGGATTCCTTTGTCATCAACATCGGTGACACTTTCATGGCTCTAACAAATGGGGTTTACAAGAGCTGCTTCCACCGAGCTGTAGTGAATTGCAAGGAAGCAAGAAAATCAATGGCCTTTTTTCTATGTCCAGCGGCTGACAAAGTGGTGAGAGCACCGGAGGAAGTGGTGGAGAAGAATCCACCACGAAAGTTTCCAGATTACACATGGCCAATGCTGCTTGAACTTACCCAAAAATTTTACAGGGCTGATTCCAATACTTTTAAGGCCTTCATACCTTGGCTTGAAGAACAAAAGTTGGGTAATACTATTGCATCACCGGCTCTTTAAATTATCCAACCCTGCCCCTTTAAA

Coding sequence (CDS)

ATGAGGGAATCAAAGGAGATGAAAAACGAGCTGCACCGTGTGGAATTTGGGTGCTCGGAGGAGTCAATGGTGCCGGAGAATTTCGTGTGGCCGGACGAGTTTAAAGCAAGGGAAGGGGCGCCGGAGCTGCATGTTCCACATATTGACTTGCAAAAGATCTTAAGTGGAGATGAAAGTGATATTGAAGAGGCAACCAGGCTGGTAGATGAAGCTTGTAGAAAGCATGGATTCTTTGTGTTGATCAACCATGGAGTGGATATGGAATTGATGAAAAAAGTTCATCAGTGTATGGATGAGTTCTTTACATTGCCTTTTGATGTCAAACAGAAATCTCAAAGGAAATTCGGTGAAAATTATGGGTATGCTAATAGCTTCATTGGAAGATTCTCCAATAATCTTCCATGGAAGGAAACTTTTTCCCTTCCTTGTGTTGCTCATCACAATTCAACCTCGGTTCAAGATTATATAGTGGAGTTATTGGGGTTAAGCCTAGGGGTACCAAAAGAGAAGTTCAAGAAATTCTATGAGGACAATGATTCAATAATAAGGCTAAATTATTACCCACCATGCGAGAAGCCAGAGCTGACATTGGGAACTGGGCCTCACTGTGATCCAACCTCCATAACAATTCTTCACCAAGACCATGTCAGTGGCCTTCAAGTCTACGTGGATGATGAGTGGCACTCAATTCCTCCAACCAAGGATTCCTTTGTCATCAACATCGGTGACACTTTCATGGCTCTAACAAATGGGGTTTACAAGAGCTGCTTCCACCGAGCTGTAGTGAATTGCAAGGAAGCAAGAAAATCAATGGCCTTTTTTCTATGTCCAGCGGCTGACAAAGTGGTGAGAGCACCGGAGGAAGTGGTGGAGAAGAATCCACCACGAAAGTTTCCAGATTACACATGGCCAATGCTGCTTGAACTTACCCAAAAATTTTACAGGGCTGATTCCAATACTTTTAAGGCCTTCATACCTTGGCTTGAAGAACAAAAGTTGGGTAATACTATTGCATCACCGGCTCTTTAA

Protein sequence

MRESKEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFSLPCVAHHNSTSVQDYIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGNTIASPAL

Homology

[Close]

BLAST of Lsi05G012810 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.2e-103
Identity = 183/338 (54.14%), Postives = 234/338 (69.23%), Query Frame = 0


Query: 21  ESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVL 80
           +S +P  F+WPDE K     PEL+VP ID    LS  +S + EA R++ EAC KHGFF++
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFID----LSSQDSTL-EAPRVIAEACTKHGFFLV 98

Query: 81  INHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFS 140
           +NHGV   L+   H+ M+ FF +P   KQK+QRK GE+ GYA+SF GRFS  LPWKET S
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 141 LPCVAHHN-STSVQDY-----------------------------IVELLGLSLGVPKEK 200
                 ++ S +VQDY                             I+ELLGLSLGV ++ 
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 201 FKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSI 260
           F+ F+E+NDSI+RLN+YPPC+ P+LTLGTGPHCDP+S+TILHQDHV+GLQV+VD++W SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 261 PPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVV 320
            P   +FV+NIGDTFMAL+NG++KSC HRAVVN + ARKSMAFFLCP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 321 EKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWL 329
           EK   RK+PD+TW M LE TQK YRAD NT  +F  W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]

BLAST of Lsi05G012810 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 376.7 bits (966), Expect = 2.8e-103
Identity = 181/338 (53.55%), Postives = 229/338 (67.75%), Query Frame = 0


Query: 21  ESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVL 80
           ++ +P  F+WPD+ K      EL VP IDLQ +LS D S   +A+RL+ EAC+KHGFF++
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96

Query: 81  INHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFS 140
           +NHG+  EL+   H+    FF +P   KQ+  RK GE+ GYA+SF GRFS  LPWKET S
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 141 LP-CVAHHNSTSVQDY-----------------------------IVELLGLSLGVPKEK 200
              C     S SVQDY                             I+ELLGLSLGV ++ 
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 201 FKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSI 260
           F++F+E+NDSI+RLNYYPPC KP+LTLGTGPHCDPTS+TILHQDHV+GLQV+V+++W SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 261 PPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVV 320
            P   +FV+NIGDTFMAL+N  YKSC HRAVVN +  RKS+AFFLCP  D+VV  P E++
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 321 EKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWL 329
           +    R++PD+TW M LE TQK YRAD NT +AF  WL
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

[Close]

BLAST of Lsi05G012810 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.3e-102
Identity = 184/342 (53.80%), Postives = 227/342 (66.37%), Query Frame = 0


Query: 24  VPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVLINH 83
           +P+ FVWPD  K       L VP IDL   LSGD     EATRLV +A  KHGFF++ NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 84  GVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFSLPC 143
           GVD  L+ + +  MD FF  P   KQK+QRK+GE+ GYA+SF+GRFS+ LPWKET S   
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156

Query: 144 VAHH--NSTSVQDY-----------------------------IVELLGLSLGVPKEKFK 203
                 +S +V+D+                             I+ELLG+SLGV +  FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216

Query: 204 KFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSIPP 263
           +F+ED+DSI RLNYYP C++PEL LGTGPHCDPTS+TILHQD V GLQV+VD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276

Query: 264 TKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVVE- 323
              +FV+NIGDTFMALTNG YKSC HRAVVN +  RK+ AFFLCP  +KVV+ PEE+V  
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336

Query: 324 -KNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQK 333
            K+  RK+PD+TW M LE TQK YRAD NT   F  WL+ ++
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378

[Close]

BLAST of Lsi05G012810 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 7.5e-101
Identity = 178/354 (50.28%), Postives = 231/354 (65.25%), Query Frame = 0


Query: 19  SEESMVPENFVWPD-EFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGF 78
           S  S +P  F+WP+ E    + A ELHVP I++  +LSGD +   E TRLV EAC +HGF
Sbjct: 12  SGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGF 71

Query: 79  FVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKE 138
           F ++NHG+D EL+   H+C+D FFT+P   KQ++ R+ GE+ GYA+SF GRF++ LPWKE
Sbjct: 72  FQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKE 131

Query: 139 TFSLPCVAHHNSTSVQDYIV-----------------------------ELLGLSLGVPK 198
           T S       +   V DYIV                             E+LG SLGV +
Sbjct: 132 TLSFRS-CPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGR 191

Query: 199 EKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWH 258
             +++F+E NDSI+RLNYYPPC++P  TLGTGPHCDPTS+TILHQD+V GLQV+ +  W 
Sbjct: 192 AHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWR 251

Query: 259 SIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEE 318
           SI P  D+FV+NIGDTFMAL+NG YKSC HRAVVN +  RKS+AFFLCP  DKVV  P  
Sbjct: 252 SIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGT 311

Query: 319 VVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGNTIASPAL 343
           +V+ + PR +PD+TW  LL+ TQK YRAD  T + F  W+ +Q+ G     PA+
Sbjct: 312 LVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQGQLALQPAM 364

[Close]

BLAST of Lsi05G012810 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.4e-100
Identity = 176/344 (51.16%), Postives = 225/344 (65.41%), Query Frame = 0


Query: 24  VPENFVWPD-EFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVLIN 83
           +P  F+WP+ E    + A ELHVP ID+  +LSGD     E TRLV EAC +HGFF ++N
Sbjct: 17  IPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVN 76

Query: 84  HGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFSLP 143
           HG+D EL+   H+C+D FFT+P   KQ++ R+ GE+ GYA+SF GRF++ LPWKET S  
Sbjct: 77  HGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFR 136

Query: 144 CVAHHNSTSVQDYIV-----------------------------ELLGLSLGVPKEKFKK 203
                +   V DYIV                             E+LG SLGV +  +++
Sbjct: 137 S-CPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRR 196

Query: 204 FYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPT 263
           F+E NDSI+RLNYYPPC++P  TLGTGPHCDPTS+TILHQD+V GLQV+ +  W SI P 
Sbjct: 197 FFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPR 256

Query: 264 KDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVVEKN 323
            D+FV+NIGDTFMAL+NG YKSC HRAVVN K  RKS+AFFLCP  DKVV  P  +V+  
Sbjct: 257 ADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAA 316

Query: 324 PPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGNTI 338
            PR +PD+TW  LL+ TQK YRAD  T + F  W+ +Q+ G  +
Sbjct: 317 NPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQGQLL 359

[Close]

BLAST of Lsi05G012810 vs. ExPASy TrEMBL
Match: Q9XHR0 (Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.2e-187
Identity = 317/369 (85.91%), Postives = 331/369 (89.70%), Query Frame = 0


Query: 3   ESKEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIE 62
           ESKEMK++LHRVEFGCSEESMVPENFVWPDEFKA++GAPELHVPHIDL K L+G+ESDIE
Sbjct: 11  ESKEMKSKLHRVEFGCSEESMVPENFVWPDEFKAKDGAPELHVPHIDLHKFLNGNESDIE 70

Query: 63  EATRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYA 122
           EATRLVDEACRKHGFFVL+NHGVDMEL+K VH+CMDEFFTLPFDVKQKSQRKFGENYGYA
Sbjct: 71  EATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQKSQRKFGENYGYA 130

Query: 123 NSFIGRFSNNLPWKETFSLPCVAHHNSTSVQDY--------------------------- 182
           NSFIGRFSNNLPWKET SLP VA+HNSTSVQD+                           
Sbjct: 131 NSFIGRFSNNLPWKETLSLPYVANHNSTSVQDFVSKCVGPELSHQGKVYQECGKALSDLS 190

Query: 183 --IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQ 242
             IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQ
Sbjct: 191 LKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQ 250

Query: 243 DHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAF 302
           DHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKE RKSMAF
Sbjct: 251 DHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEVRKSMAF 310

Query: 303 FLCPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKL 343
           FLCPAADKVVRAP+EVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTF+AFIPWLE+QK 
Sbjct: 311 FLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFQAFIPWLEKQKS 370

[Close]

BLAST of Lsi05G012810 vs. ExPASy TrEMBL
Match: A0A6J1J263 (gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482738 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.7e-165
Identity = 281/366 (76.78%), Postives = 304/366 (83.06%), Query Frame = 0


Query: 5   KEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEA 64
           +EMK + HRVEFG S+ESMVPENF+WPDEFKARE  PEL VPHIDLQK +S +E+DIEEA
Sbjct: 19  QEMKRK-HRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKFVSENENDIEEA 78

Query: 65  TRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANS 124
           TRLVDEACRKHGFFVL+NHGVDMELMK VH+CMDEFF+LP DVKQK+QRK GENYGYANS
Sbjct: 79  TRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKVGENYGYANS 138

Query: 125 FIGRFSNNLPWKETFSLPCVAHHNSTSVQDY----------------------------- 184
           FIGRFS+ LPWKETFSL   AHHN     DY                             
Sbjct: 139 FIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQGKVYEECGKALSDLSLK 198

Query: 185 IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDH 244
           IVELLGLSLGVPKEKFKKF+EDNDSIIRLNYYPPC KPELTLGTGPHCDPTSITILHQDH
Sbjct: 199 IVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPTSITILHQDH 258

Query: 245 VSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFL 304
           VSGLQVYVDD+WHSIPP KDSFVINIGDTFMALTNGVYKSCFHRAVVNC+EARKS+ FFL
Sbjct: 259 VSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCREARKSIVFFL 318

Query: 305 CPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGN 342
           CPA DKVVRAP+E+V+KNPPRKFPDYTWPMLLELTQKFYR+DSNTFKAF  WL+ + L  
Sbjct: 319 CPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTTWLQHKNLAT 378

[Close]

BLAST of Lsi05G012810 vs. ExPASy TrEMBL
Match: I0J1I7 (Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox4 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.7e-165
Identity = 281/366 (76.78%), Postives = 304/366 (83.06%), Query Frame = 0


Query: 5   KEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEA 64
           +EMK + HRVEFG S+ESMVPENF+WPDEFKARE  PEL VPHIDLQK +S +E+DIEEA
Sbjct: 19  QEMKRK-HRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKFVSENENDIEEA 78

Query: 65  TRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANS 124
           TRLVDEACRKHGFFVL+NHGVDMELMK VH+CMDEFF+LP DVKQK+QRK GENYGYANS
Sbjct: 79  TRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKVGENYGYANS 138

Query: 125 FIGRFSNNLPWKETFSLPCVAHHNSTSVQDY----------------------------- 184
           FIGRFS+ LPWKETFSL   AHHN     DY                             
Sbjct: 139 FIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQGKVYEECGEALSDLSLK 198

Query: 185 IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDH 244
           IVELLGLSLGVPKEKFKKF+EDNDSIIRLNYYPPC KPELTLGTGPHCDPTSITILHQDH
Sbjct: 199 IVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPTSITILHQDH 258

Query: 245 VSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFL 304
           VSGLQVYVDD+WHSIPP KDSFVINIGDTFMALTNGVYKSCFHRAVVNC+EARKS+ FFL
Sbjct: 259 VSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCREARKSIVFFL 318

Query: 305 CPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGN 342
           CPA DKVVRAP+E+V+KNPPRKFPDYTWPMLLELTQKFYR+DSNTFKAF  WL+ + L  
Sbjct: 319 CPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTTWLQHKNLAT 378

[Close]

BLAST of Lsi05G012810 vs. ExPASy TrEMBL
Match: A0A5A7SQY2 (Gibberellin 20 oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00120 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.1e-163
Identity = 284/366 (77.60%), Postives = 307/366 (83.88%), Query Frame = 0


Query: 8   KNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRL 67
           ++   RVEFGCSEESMVPE FVWPDEFK +EG PEL VP IDLQK LSG+ESDIEE  RL
Sbjct: 11  RDSTQRVEFGCSEESMVPEKFVWPDEFKPKEGLPELQVPKIDLQKFLSGNESDIEETIRL 70

Query: 68  VDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIG 127
           VDEAC+KHGFF+L+NHGVDMELMKK+H CM+EFFT PFDVKQK+QRKFGEN+GYANSFIG
Sbjct: 71  VDEACKKHGFFLLVNHGVDMELMKKLHGCMEEFFTFPFDVKQKAQRKFGENFGYANSFIG 130

Query: 128 RFSNNLPWKETFSLPCVAHHNSTSVQDY-----------------------------IVE 187
           RFSNNLPWKET S+P VA   ST+  DY                             IVE
Sbjct: 131 RFSNNLPWKETLSVPYVADQKSTA-HDYILEIYGNELSHHGKVYQECGEALSELGLKIVE 190

Query: 188 LLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSG 247
           LLGLSLGVPKE+F+KFYEDN+SI+RLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSG
Sbjct: 191 LLGLSLGVPKERFRKFYEDNESIMRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSG 250

Query: 248 LQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPA 307
           LQVYVDDEWH+IPPT DSFVINIGDTFMALTNGVYKSC HRAVVNCKEARKSMAFFL PA
Sbjct: 251 LQVYVDDEWHTIPPTTDSFVINIGDTFMALTNGVYKSCLHRAVVNCKEARKSMAFFLNPA 310

Query: 308 ADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEE-QKLGN-T 343
           ADKVVRAP+EVVEKNPPRKFPD+TWPMLL+LTQKFYR+DSNT KAFIPWLEE QKL N T
Sbjct: 311 ADKVVRAPDEVVEKNPPRKFPDFTWPMLLQLTQKFYRSDSNTLKAFIPWLEEQQKLANDT 370

[Close]

BLAST of Lsi05G012810 vs. ExPASy TrEMBL
Match: A0A6J1E4L1 (gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.2e-163
Identity = 279/366 (76.23%), Postives = 304/366 (83.06%), Query Frame = 0


Query: 5   KEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEA 64
           +EMK + HRVEFG S+ESMVPENF+WP+EFKARE  PELHVPHIDLQK +S +E+DIEE 
Sbjct: 19  QEMKRK-HRVEFGSSDESMVPENFIWPEEFKARESVPELHVPHIDLQKFMSENENDIEEV 78

Query: 65  TRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANS 124
            RLVDEACRKHGFFVL+NHGVDMELMK VH+CMDEFF+LP DVKQK+QRK GENYGYANS
Sbjct: 79  RRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKVGENYGYANS 138

Query: 125 FIGRFSNNLPWKETFSLPCVAHHNSTSVQDY----------------------------- 184
           FIGRFS+ LPWKETFSL   AHH  T   DY                             
Sbjct: 139 FIGRFSSKLPWKETFSLRYAAHHPIT--HDYVCHHLGQEFSQQGKVYEECGKALSDLSLK 198

Query: 185 IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDH 244
           IVELLGLSLGVPK+KFKKF+EDNDSIIRLNYYPPC KPELTLGTGPHCDPTSITILHQDH
Sbjct: 199 IVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPTSITILHQDH 258

Query: 245 VSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFL 304
           VSGLQVYVDD+WHSIPP KDSFVINIGDTFMALTNGVYKSCFHRAVVNC+EARKS+ FFL
Sbjct: 259 VSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCREARKSIVFFL 318

Query: 305 CPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGN 342
           CPA DKVVRAP+E+V+KNPPRKFPDYTWPMLLELTQKFYR+DSNTFKAF  WL+ +KL  
Sbjct: 319 CPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTTWLQHKKLAT 378

[Close]

BLAST of Lsi05G012810 vs. NCBI nr
Match: AAD42693.1 (gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 663.7 bits (1711), Expect = 8.6e-187
Identity = 317/369 (85.91%), Postives = 331/369 (89.70%), Query Frame = 0


Query: 3   ESKEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIE 62
           ESKEMK++LHRVEFGCSEESMVPENFVWPDEFKA++GAPELHVPHIDL K L+G+ESDIE
Sbjct: 11  ESKEMKSKLHRVEFGCSEESMVPENFVWPDEFKAKDGAPELHVPHIDLHKFLNGNESDIE 70

Query: 63  EATRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYA 122
           EATRLVDEACRKHGFFVL+NHGVDMEL+K VH+CMDEFFTLPFDVKQKSQRKFGENYGYA
Sbjct: 71  EATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQKSQRKFGENYGYA 130

Query: 123 NSFIGRFSNNLPWKETFSLPCVAHHNSTSVQDY--------------------------- 182
           NSFIGRFSNNLPWKET SLP VA+HNSTSVQD+                           
Sbjct: 131 NSFIGRFSNNLPWKETLSLPYVANHNSTSVQDFVSKCVGPELSHQGKVYQECGKALSDLS 190

Query: 183 --IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQ 242
             IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQ
Sbjct: 191 LKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQ 250

Query: 243 DHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAF 302
           DHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKE RKSMAF
Sbjct: 251 DHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEVRKSMAF 310

Query: 303 FLCPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKL 343
           FLCPAADKVVRAP+EVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTF+AFIPWLE+QK 
Sbjct: 311 FLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFQAFIPWLEKQKS 370

[Close]

BLAST of Lsi05G012810 vs. NCBI nr
Match: XP_038905755.1 (gibberellin 20 oxidase 2-like [Benincasa hispida])

HSP 1 Score: 628.6 bits (1620), Expect = 3.1e-176
Identity = 297/360 (82.50%), Postives = 317/360 (88.06%), Query Frame = 0


Query: 13  RVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEAC 72
           RVEFGCSEESMVPE FVWPDEFK +EGAPELHVPHIDLQK LSG++SDIEEATR VDEAC
Sbjct: 15  RVEFGCSEESMVPETFVWPDEFKPKEGAPELHVPHIDLQKFLSGNQSDIEEATRRVDEAC 74

Query: 73  RKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNN 132
           RKHGFFVL+NHGVDMELMKK+HQCMDE+FTLPFDVK+K+QRK GEN+GYANSFIGRFS+N
Sbjct: 75  RKHGFFVLVNHGVDMELMKKLHQCMDEYFTLPFDVKKKAQRKLGENFGYANSFIGRFSHN 134

Query: 133 LPWKETFSLPCVAHHNSTSVQDY-----------------------------IVELLGLS 192
           LPWKETFSLP VAHHNST+  DY                             IVELL LS
Sbjct: 135 LPWKETFSLPYVAHHNSTTTLDYFSQYYGSEFSHQGKVYQECGEALSDLSLKIVELLALS 194

Query: 193 LGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYV 252
           LG PKEKF+KFYEDN SI+RLNYYPPCEKPELTLGTGPHCDPTS+TILHQDHVSGLQVYV
Sbjct: 195 LGAPKEKFRKFYEDNSSIMRLNYYPPCEKPELTLGTGPHCDPTSVTILHQDHVSGLQVYV 254

Query: 253 DDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVV 312
           DDEWHSIPPTKDSFVIN+GDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVV
Sbjct: 255 DDEWHSIPPTKDSFVINVGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVV 314

Query: 313 RAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKL-GNTIASPAL 343
           RAPEEVV+KNPPRKFPD+TWP+LLELTQKFYRADSNTFKAFIPWLEEQKL  NTIA P++
Sbjct: 315 RAPEEVVDKNPPRKFPDFTWPLLLELTQKFYRADSNTFKAFIPWLEEQKLANNTIAPPSV 374

[Close]

BLAST of Lsi05G012810 vs. NCBI nr
Match: XP_022984437.1 (gibberellin 20 oxidase 2-like [Cucurbita maxima])

HSP 1 Score: 589.7 bits (1519), Expect = 1.6e-164
Identity = 281/366 (76.78%), Postives = 304/366 (83.06%), Query Frame = 0


Query: 5   KEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEA 64
           +EMK + HRVEFG S+ESMVPENF+WPDEFKARE  PEL VPHIDLQK +S +E+DIEEA
Sbjct: 19  QEMKRK-HRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKFVSENENDIEEA 78

Query: 65  TRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANS 124
           TRLVDEACRKHGFFVL+NHGVDMELMK VH+CMDEFF+LP DVKQK+QRK GENYGYANS
Sbjct: 79  TRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKVGENYGYANS 138

Query: 125 FIGRFSNNLPWKETFSLPCVAHHNSTSVQDY----------------------------- 184
           FIGRFS+ LPWKETFSL   AHHN     DY                             
Sbjct: 139 FIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQGKVYEECGKALSDLSLK 198

Query: 185 IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDH 244
           IVELLGLSLGVPKEKFKKF+EDNDSIIRLNYYPPC KPELTLGTGPHCDPTSITILHQDH
Sbjct: 199 IVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPTSITILHQDH 258

Query: 245 VSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFL 304
           VSGLQVYVDD+WHSIPP KDSFVINIGDTFMALTNGVYKSCFHRAVVNC+EARKS+ FFL
Sbjct: 259 VSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCREARKSIVFFL 318

Query: 305 CPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGN 342
           CPA DKVVRAP+E+V+KNPPRKFPDYTWPMLLELTQKFYR+DSNTFKAF  WL+ + L  
Sbjct: 319 CPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTTWLQHKNLAT 378

[Close]

BLAST of Lsi05G012810 vs. NCBI nr
Match: CBL59326.1 (Gibberellin 20-oxidase [Cucurbita maxima])

HSP 1 Score: 589.7 bits (1519), Expect = 1.6e-164
Identity = 281/366 (76.78%), Postives = 304/366 (83.06%), Query Frame = 0


Query: 5   KEMKNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEA 64
           +EMK + HRVEFG S+ESMVPENF+WPDEFKARE  PEL VPHIDLQK +S +E+DIEEA
Sbjct: 19  QEMKRK-HRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKFVSENENDIEEA 78

Query: 65  TRLVDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANS 124
           TRLVDEACRKHGFFVL+NHGVDMELMK VH+CMDEFF+LP DVKQK+QRK GENYGYANS
Sbjct: 79  TRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKVGENYGYANS 138

Query: 125 FIGRFSNNLPWKETFSLPCVAHHNSTSVQDY----------------------------- 184
           FIGRFS+ LPWKETFSL   AHHN     DY                             
Sbjct: 139 FIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQGKVYEECGEALSDLSLK 198

Query: 185 IVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDH 244
           IVELLGLSLGVPKEKFKKF+EDNDSIIRLNYYPPC KPELTLGTGPHCDPTSITILHQDH
Sbjct: 199 IVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPTSITILHQDH 258

Query: 245 VSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFL 304
           VSGLQVYVDD+WHSIPP KDSFVINIGDTFMALTNGVYKSCFHRAVVNC+EARKS+ FFL
Sbjct: 259 VSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCREARKSIVFFL 318

Query: 305 CPAADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQKLGN 342
           CPA DKVVRAP+E+V+KNPPRKFPDYTWPMLLELTQKFYR+DSNTFKAF  WL+ + L  
Sbjct: 319 CPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTTWLQHKNLAT 378

[Close]

BLAST of Lsi05G012810 vs. NCBI nr
Match: KAA0033594.1 (gibberellin 20 oxidase 2-like [Cucumis melo var. makuwa] >TYK22260.1 gibberellin 20 oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 585.9 bits (1509), Expect = 2.3e-163
Identity = 284/366 (77.60%), Postives = 307/366 (83.88%), Query Frame = 0


Query: 8   KNELHRVEFGCSEESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRL 67
           ++   RVEFGCSEESMVPE FVWPDEFK +EG PEL VP IDLQK LSG+ESDIEE  RL
Sbjct: 11  RDSTQRVEFGCSEESMVPEKFVWPDEFKPKEGLPELQVPKIDLQKFLSGNESDIEETIRL 70

Query: 68  VDEACRKHGFFVLINHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIG 127
           VDEAC+KHGFF+L+NHGVDMELMKK+H CM+EFFT PFDVKQK+QRKFGEN+GYANSFIG
Sbjct: 71  VDEACKKHGFFLLVNHGVDMELMKKLHGCMEEFFTFPFDVKQKAQRKFGENFGYANSFIG 130

Query: 128 RFSNNLPWKETFSLPCVAHHNSTSVQDY-----------------------------IVE 187
           RFSNNLPWKET S+P VA   ST+  DY                             IVE
Sbjct: 131 RFSNNLPWKETLSVPYVADQKSTA-HDYILEIYGNELSHHGKVYQECGEALSELGLKIVE 190

Query: 188 LLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSG 247
           LLGLSLGVPKE+F+KFYEDN+SI+RLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSG
Sbjct: 191 LLGLSLGVPKERFRKFYEDNESIMRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSG 250

Query: 248 LQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPA 307
           LQVYVDDEWH+IPPT DSFVINIGDTFMALTNGVYKSC HRAVVNCKEARKSMAFFL PA
Sbjct: 251 LQVYVDDEWHTIPPTTDSFVINIGDTFMALTNGVYKSCLHRAVVNCKEARKSMAFFLNPA 310

Query: 308 ADKVVRAPEEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEE-QKLGN-T 343
           ADKVVRAP+EVVEKNPPRKFPD+TWPMLL+LTQKFYR+DSNT KAFIPWLEE QKL N T
Sbjct: 311 ADKVVRAPDEVVEKNPPRKFPDFTWPMLLQLTQKFYRSDSNTLKAFIPWLEEQQKLANDT 370

[Close]

BLAST of Lsi05G012810 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 377.9 bits (969), Expect = 8.8e-105
Identity = 183/338 (54.14%), Postives = 234/338 (69.23%), Query Frame = 0


Query: 21  ESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVL 80
           +S +P  F+WPDE K     PEL+VP ID    LS  +S + EA R++ EAC KHGFF++
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFID----LSSQDSTL-EAPRVIAEACTKHGFFLV 98

Query: 81  INHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFS 140
           +NHGV   L+   H+ M+ FF +P   KQK+QRK GE+ GYA+SF GRFS  LPWKET S
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 141 LPCVAHHN-STSVQDY-----------------------------IVELLGLSLGVPKEK 200
                 ++ S +VQDY                             I+ELLGLSLGV ++ 
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 201 FKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSI 260
           F+ F+E+NDSI+RLN+YPPC+ P+LTLGTGPHCDP+S+TILHQDHV+GLQV+VD++W SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 261 PPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVV 320
            P   +FV+NIGDTFMAL+NG++KSC HRAVVN + ARKSMAFFLCP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 321 EKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWL 329
           EK   RK+PD+TW M LE TQK YRAD NT  +F  W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]

BLAST of Lsi05G012810 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.0e-104
Identity = 181/338 (53.55%), Postives = 229/338 (67.75%), Query Frame = 0


Query: 21  ESMVPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVL 80
           ++ +P  F+WPD+ K      EL VP IDLQ +LS D S   +A+RL+ EAC+KHGFF++
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96

Query: 81  INHGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFS 140
           +NHG+  EL+   H+    FF +P   KQ+  RK GE+ GYA+SF GRFS  LPWKET S
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 141 LP-CVAHHNSTSVQDY-----------------------------IVELLGLSLGVPKEK 200
              C     S SVQDY                             I+ELLGLSLGV ++ 
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 201 FKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSI 260
           F++F+E+NDSI+RLNYYPPC KP+LTLGTGPHCDPTS+TILHQDHV+GLQV+V+++W SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 261 PPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVV 320
            P   +FV+NIGDTFMAL+N  YKSC HRAVVN +  RKS+AFFLCP  D+VV  P E++
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 321 EKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWL 329
           +    R++PD+TW M LE TQK YRAD NT +AF  WL
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

[Close]

BLAST of Lsi05G012810 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 373.6 bits (958), Expect = 1.7e-103
Identity = 184/342 (53.80%), Postives = 227/342 (66.37%), Query Frame = 0


Query: 24  VPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVLINH 83
           +P+ FVWPD  K       L VP IDL   LSGD     EATRLV +A  KHGFF++ NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 84  GVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFSLPC 143
           GVD  L+ + +  MD FF  P   KQK+QRK+GE+ GYA+SF+GRFS+ LPWKET S   
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156

Query: 144 VAHH--NSTSVQDY-----------------------------IVELLGLSLGVPKEKFK 203
                 +S +V+D+                             I+ELLG+SLGV +  FK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216

Query: 204 KFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSIPP 263
           +F+ED+DSI RLNYYP C++PEL LGTGPHCDPTS+TILHQD V GLQV+VD++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276

Query: 264 TKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVVE- 323
              +FV+NIGDTFMALTNG YKSC HRAVVN +  RK+ AFFLCP  +KVV+ PEE+V  
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336

Query: 324 -KNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQK 333
            K+  RK+PD+TW M LE TQK YRAD NT   F  WL+ ++
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378

[Close]

BLAST of Lsi05G012810 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 362.1 bits (928), Expect = 5.0e-100
Identity = 178/341 (52.20%), Postives = 221/341 (64.81%), Query Frame = 0


Query: 24  VPENFVWPDEFKAREGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVLINH 83
           +P+ FVWPD  K  +  P L VP IDL   LS D   + EA RLV EA +KHGFF++ NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 84  GVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFSL-- 143
           GVD  L+   H+ MD FF  P   K K+QRK GE  GYA+SF+GRF  NLPWKET S   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 144 -PCVAHHN-STSVQDY-----------------------------IVELLGLSLGVPKEK 203
            P     N S +V++Y                             I+ELLG+SLG+ +E 
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 204 FKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSI 263
           F++F+EDN+SI RLNYYP C++P+L LGTGPHCDPTS+TIL QD VSGLQV+VD++W SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 264 PPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVV 323
           PP   + V+NIGDT MALTNG+YKSC HRAVVN +  RK++AFFLCP  DKVV+ P E+ 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 324 EKNPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWLEEQ 332
                R +PD+TW M LE T K YRAD NT + F  WL+ +
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLKNK 373

[Close]

BLAST of Lsi05G012810 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 261.5 bits (667), Expect = 9.2e-70
Identity = 138/336 (41.07%), Postives = 196/336 (58.33%), Query Frame = 0


Query: 24  VPENFVWPDEFKA-REGAPELHVPHIDLQKILSGDESDIEEATRLVDEACRKHGFFVLIN 83
           VP  F WP++  A  EG  +L +P IDL   L+G+E++ + A + V +AC  HG F+++N
Sbjct: 43  VPAEFFWPEKDVAPSEG--DLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVN 102

Query: 84  HGVDMELMKKVHQCMDEFFTLPFDVKQKSQRKFGENYGYANSFIGRFSNNLPWKETFSL- 143
           HG    L +K  +    FF L  D K ++ R  G   GY      RFS+NLPW ET +L 
Sbjct: 103 HGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLTLA 162

Query: 144 -----PCVAHHNSTS------------VQDY----------IVELLGLSLGV-PKEKFKK 203
                P V     TS             Q++          ++ELLG+S+G+  +  +++
Sbjct: 163 FKKGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTYYRR 222

Query: 204 FYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPT 263
           F+ED   I R NYYPPC++PE  LG GPH DPT+IT+L QD V GL+V+    W ++ P 
Sbjct: 223 FFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTVRPR 282

Query: 264 KDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEARKSMAFFLCPAADKVVRAPEEVVE-K 323
             + V+N+GDTFMAL+NG Y+SC+HRAVVN ++ R+S+ FF CP  DK++  P E+VE +
Sbjct: 283 PGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELVEGE 342

Query: 324 NPPRKFPDYTWPMLLELTQKFYRADSNTFKAFIPWL 329
              RK+PD+TW  L + TQ  YR D+ T   F  WL
Sbjct: 343 EASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWL 376

The following BLAST results are available for this feature:

BLAST of Lsi05G012810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39111	1.2e-103	54.14	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
Q39110	2.8e-103	53.55	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
Q39112	2.3e-102	53.80	Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1	[more]
O04707	7.5e-101	50.28	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]
O04706	6.4e-100	51.16	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]

BLAST of Lsi05G012810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9XHR0	4.2e-187	85.91	Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1	[more]
A0A6J1J263	7.7e-165	76.78	gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482738 PE=3 S...	[more]
I0J1I7	7.7e-165	76.78	Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox4 PE=2 SV=1	[more]
A0A5A7SQY2	1.1e-163	77.60	Gibberellin 20 oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A6J1E4L1	3.2e-163	76.23	gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3...	[more]

BLAST of Lsi05G012810 vs. NCBI nr


Match Name	E-value	Identity	Description
AAD42693.1	8.6e-187	85.91	gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris]	[more]
XP_038905755.1	3.1e-176	82.50	gibberellin 20 oxidase 2-like [Benincasa hispida]	[more]
XP_022984437.1	1.6e-164	76.78	gibberellin 20 oxidase 2-like [Cucurbita maxima]	[more]
CBL59326.1	1.6e-164	76.78	Gibberellin 20-oxidase [Cucurbita maxima]	[more]
KAA0033594.1	2.3e-163	77.60	gibberellin 20 oxidase 2-like [Cucumis melo var. makuwa] >TYK22260.1 gibberellin...	[more]

BLAST of Lsi05G012810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51810.1	8.8e-105	54.14	gibberellin 20 oxidase 2	[more]
AT4G25420.1	2.0e-104	53.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G07200.1	1.7e-103	53.80	gibberellin 20-oxidase 3	[more]
AT1G60980.1	5.0e-100	52.20	gibberellin 20-oxidase 4	[more]
AT1G44090.1	9.2e-70	41.07	gibberellin 20-oxidase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 179..277 e-value: 5.4E-29 score: 100.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 45..144 e-value: 4.3E-23 score: 82.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 15..313 e-value: 3.5E-80 score: 271.8
None	No IPR available	PANTHER	PTHR47990:SF26	GIBBERELLIN 20-OXIDASE	coord: 156..329
None	No IPR available	PANTHER	PTHR47990:SF26	GIBBERELLIN 20-OXIDASE	coord: 17..158
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 17..158
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 156..329
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 19..315
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 177..277 score: 14.037087

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G012810.1	Lsi05G012810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009908	flower development
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0009826	unidimensional cell growth
molecular_function	GO:0045544	gibberellin 20-oxidase activity
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding