Lsi05G012530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G012530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein IQ-DOMAIN 14
Locationchr05: 20312916 .. 20316671 (-)
RNA-Seq ExpressionLsi05G012530
SyntenyLsi05G012530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGTGCTCACATCACGATTCCCATTTCCCAAATCCAACCTCACTTCTTCTTCTTCTTCTTCTGCTTCTTCCCCAAATTAAAACTCTCACACTCACTTTCTTCTCTTCTTCCATTCCTTTCTACTCTCTCATATTTTCTCAGCCATGCTTTTAATTTTCTACTCCTCATTTTCCTAGCTTTCCATTTCCATTTATGGGCTTTCTCCGGCGACTTTTCCGTCCAAAAAAGCCCCCTCCCGACCCCCCGGACTTCACCCCAGACCCCAACAAGAAGAAAAACAAGCGGTGGAGCTTCCCCAAGTCCGCCCACAACCACCACTCTCGCCCTTACGCCACCCCCTCCCAACCCAATGCATTTGGCCCCTCCTCTTCCTACACAGAACCTCTCGATGCCAACAAGCACGCGATTGCTGTAGCTGCCGCCACCGCCGCCGTCGCCGAGGCAGCTCTAGCCGCCGCCCATGCTGCTGCCGAGGTTGTTCGGCTCACCAGCAGCGGCACCACTCACGCTAATGCTAACCGCCGTTGGATGGAGGAGGCGGCTGCCGTTAAAATCCAGTCCGCCTTTCGAGGTTATTTGGTGAGTTGGCTCCTTTTCTCTCGTCTTACATTCTTTCTTTAGAAATTTCCCCTTCCATTATTGCTGTAATTATTCTCATTGACCTTCAATAGAGGAAGATTTTGGCAATGTTGGGGGTCAGCCTTTGTTGGAGACTTCCATTTTTTACCTTCTTGAGTTACTTCCATATGGGGTTTGGCTGGTGATAATTTTTCAAACTTGATTTTTTAGAAAGTTTGTGATTAGCCGTAGTTTTCTAGAATTTGATTTCATTTGTGTTCAAACCTAAACTTTGAATTACCCCTTTTCCCTGTAGACCCAAATGCAAAGAAACTTACCTAATCTGCAACTCCTACATTTCTTTCAAAAAGCAACTTTTATCTTTTCAAACAGAAAAAGAAAAGAAAAGAAAAGAAAAGAAAAACCTTCACATGGGCATTAGGGATTGTATTTCCATGTTCCTTCACCTGATGCTTCACTTTCCCTTTCTCTTTTGTTCTGCTCTGGATTTCAATCTAAAACCAAGCACAAAACATAAAGTGGTCCTAAGAATTTTCCCGGAAAATTAAGTGTATAGAAAGAAATTGAGTTGGGTTATTGCGCCGACACAAGTGGATGTGTGATCTCAGCATCTCAGAACATCCAACTCCCTATTGTCCACGCATGTCCAATTGTGTTGTATCTCCATCCACTGTTCTAATGGTTGCACGTGTATTGGGTCAAACACGTGTTCACATCTTGCTGTTTCCTTCACCAATTCCATTAGCTACCAGTTTTTGTGGTAGTGGCTGTAGGAGATTGATTATACTTTGATGACATGATTCCACTTCCTCTTATCAAGAATCTTCCAAGTTTAATGTAATTTCTTATTGCTTTGCCATAATCACAAAAGACATTGCTGGGTCCTTCCTTCCCTTTCAAGTTGGTTTTTCTTTTCCCTTAATAATTAATCCAATGGATCATTGCCAGCTGCGCTTCTCGTTTGATTTTTAGAGTTTAACAAGTTTTAGGGGATTTGTATCTGAAATTCAGGGGAGAAATGTATAATTAATTAAATGGTCTGCTTAAAATAGCAAAAGTGACTTTATCATAGCTTTTGTTTATTTCAAATGGTTGTATTAAAGCTGCAGTTTGGCATTGGTTGAATTTCTTTTATGCATTGGTCCCCTTCCCCCATCATAGCTCTTTCCACATTTAGTTTACAGAATAATTGTTGTTCAAGGGACTTTTTGAATATGAGTTGCAGAAAGAGTTTTGGCTATTATTGTGTCTAACTTTTGGAGGGAAATTTTGAAGGCAAGGAGGGCACTTAGGGCACTAAAGGCATTGGTAAAGCTTCAAGCTTTGGTGAGAGGTCACATTGTGAGAAAGCAAATGGCTGACATGTTAAGGCGAATGCAGACACTGGTTAGGTTGCAATCTCGAGCTTGTGCTGGTCGTTCAAACTTATCAGATTCTTTGCATTCTACCAGCAAATCCTCGCTCTCTCATATCCGTGTATGTGTTCATTTTCCCCTCCCATTCATAGCCAGTTTCCAATATTGTCATGCATTTTTTACTGCCTTCCTTTTAGTTTGTAGCTTTCTGGGATTCTTAAGCTTGTATTGAATTAATTGTTCTGTTTGAATAGGAAAGAGCTAAGAATCTGGTCAACTAAAATGTGGAAGTTCTGTTTGCCTCCTGACTTTATGAGTTTATGTAATCTCTGTTAGTCTGTAGCAATACTGCATATAACTTTTTAACATAACCTATTGCCATTATTAGAGAATGAGGTTTCCATGAGACCTCATGGAATAAACTATTCCATGAGACTTCATGGAATAGTTTCCTTTTCTCTGTTGGTTACTTGTTTGATGCACTTAAGTTTGTGCTTTTATTTATCTCCATTTTTCCGAAAATCCTCACCGTTGGTTTCTTTTTATTGATAGGTCCAAGCAACCCCCAATGGAAATGAACATCAAATCAGTGCTCATCATGGTAATAAATTTGACAACTCTGCACTTCTGAAGGTATACAATTTAATGTCATTTGCAGCTGAATGACTTTAATTTCTTTTGTCTTTTTAAGCTCTAATATTACTAATCATTCATATTTTTGTTTTATATTCTATAATGTTTAAATTCATTCAAGTTATTTGCAGACTCTGATGTTGCAACCAGATTCTGATGCTTGAATATTTTACCCAAAAAGTAACATTCATTTTCAACTTTTCCTACCAACTCTTGCAGAGATGTGGTTCGAATTCAAATTTGAAGGATGTAACTGTTGTGGATAGAGCACCAGTCGGGTCGAGTTGGTTAGACCGTTGGATGGAAGAAAACTTGTGGAACGATCGAGAGTTGCCTCTAAAAAATATTCATGCTATAGATGAGAAAACAGACAAAATCCTTGAAGTAGATACATGGAAACCTCATTTCAAGTCTCAAGGAAACATCAGCAGCTTTAAAAATTCTCAAATGGCTCCAGATTTTAAAAACCACCAGAACTTTACAACGATTGACTCCCCATCAAAACACTCGTCAAAGGCTGCAAATCCAGTTTCGAGTCTTTCCTCAGGGGAAGCATCCTTGAGCTCCTTAAAATTCCCTGTTGGAAAATATGAAACAGCTCCAAGAACAGCAGAAAATAGCCCTCAAGTGCATTCTGCTTCATCCAGGCGTGGAAATGGCGCAAAGAGAGCTACCTTGTCGCCGACAAGAAGTGAGTATGCATGGGGATATTTCAGTGGCTATGCGGGTTATCCAAACTATATGGCCAACACAGAGTCATCCAAAGCAAAAGTCAGGTCACAAAGTGCCCCGAAACAGAGGGTCGAGTTAGAGAAATTTGGATCGAACAAGCGGTATGCTCAGGTATCTTGGGATGCATGGAGCTTTTCAAACAATGGTATCTCCCATGAACTCAATTCATCAAACAATGCCAATTCAGTAGCTGATCGCATGACTAAGTTTGCAAGTACCAAATCAAGATGATCTTTATTTGTTTTTGTAACTTCTCTTTTTTGCTTATGCTGTGTGTGAGCGTGTGCTTTCTTTCTATTCAGCACCAATGAAATTGTAATTATTTGACCACTTCTGATGTCTAGTCTACAACTTGTTGACCATGAACAGACAATACTTCCATGTTTCGAGTGCTACCCAATTCCTTTGAAATTTGAAATTGTACTCCGATATCATTAATCAACCTCTGTTCCATCT

mRNA sequence

AAGGTGCTCACATCACGATTCCCATTTCCCAAATCCAACCTCACTTCTTCTTCTTCTTCTTCTGCTTCTTCCCCAAATTAAAACTCTCACACTCACTTTCTTCTCTTCTTCCATTCCTTTCTACTCTCTCATATTTTCTCAGCCATGCTTTTAATTTTCTACTCCTCATTTTCCTAGCTTTCCATTTCCATTTATGGGCTTTCTCCGGCGACTTTTCCGTCCAAAAAAGCCCCCTCCCGACCCCCCGGACTTCACCCCAGACCCCAACAAGAAGAAAAACAAGCGGTGGAGCTTCCCCAAGTCCGCCCACAACCACCACTCTCGCCCTTACGCCACCCCCTCCCAACCCAATGCATTTGGCCCCTCCTCTTCCTACACAGAACCTCTCGATGCCAACAAGCACGCGATTGCTGTAGCTGCCGCCACCGCCGCCGTCGCCGAGGCAGCTCTAGCCGCCGCCCATGCTGCTGCCGAGGTTGTTCGGCTCACCAGCAGCGGCACCACTCACGCTAATGCTAACCGCCGTTGGATGGAGGAGGCGGCTGCCGTTAAAATCCAGTCCGCCTTTCGAGGTTATTTGGCAAGGAGGGCACTTAGGGCACTAAAGGCATTGGTAAAGCTTCAAGCTTTGGTGAGAGGTCACATTGTGAGAAAGCAAATGGCTGACATGTTAAGGCGAATGCAGACACTGGTTAGGTTGCAATCTCGAGCTTGTGCTGGTCGTTCAAACTTATCAGATTCTTTGCATTCTACCAGCAAATCCTCGCTCTCTCATATCCGTGTCCAAGCAACCCCCAATGGAAATGAACATCAAATCAGTGCTCATCATGGTAATAAATTTGACAACTCTGCACTTCTGAAGAGATGTGGTTCGAATTCAAATTTGAAGGATGTAACTGTTGTGGATAGAGCACCAGTCGGGTCGAGTTGGTTAGACCGTTGGATGGAAGAAAACTTGTGGAACGATCGAGAGTTGCCTCTAAAAAATATTCATGCTATAGATGAGAAAACAGACAAAATCCTTGAAGTAGATACATGGAAACCTCATTTCAAGTCTCAAGGAAACATCAGCAGCTTTAAAAATTCTCAAATGGCTCCAGATTTTAAAAACCACCAGAACTTTACAACGATTGACTCCCCATCAAAACACTCGTCAAAGGCTGCAAATCCAGTTTCGAGTCTTTCCTCAGGGGAAGCATCCTTGAGCTCCTTAAAATTCCCTGTTGGAAAATATGAAACAGCTCCAAGAACAGCAGAAAATAGCCCTCAAGTGCATTCTGCTTCATCCAGGCGTGGAAATGGCGCAAAGAGAGCTACCTTGTCGCCGACAAGAAGTGAGTATGCATGGGGATATTTCAGTGGCTATGCGGGTTATCCAAACTATATGGCCAACACAGAGTCATCCAAAGCAAAAGTCAGGTCACAAAGTGCCCCGAAACAGAGGGTCGAGTTAGAGAAATTTGGATCGAACAAGCGGTATGCTCAGGTATCTTGGGATGCATGGAGCTTTTCAAACAATGGTATCTCCCATGAACTCAATTCATCAAACAATGCCAATTCAGTAGCTGATCGCATGACTAAGTTTGCAAGTACCAAATCAAGATGATCTTTATTTGTTTTTGTAACTTCTCTTTTTTGCTTATGCTGTGTGTGAGCGTGTGCTTTCTTTCTATTCAGCACCAATGAAATTGTAATTATTTGACCACTTCTGATGTCTAGTCTACAACTTGTTGACCATGAACAGACAATACTTCCATGTTTCGAGTGCTACCCAATTCCTTTGAAATTTGAAATTGTACTCCGATATCATTAATCAACCTCTGTTCCATCT

Coding sequence (CDS)

ATGGGCTTTCTCCGGCGACTTTTCCGTCCAAAAAAGCCCCCTCCCGACCCCCCGGACTTCACCCCAGACCCCAACAAGAAGAAAAACAAGCGGTGGAGCTTCCCCAAGTCCGCCCACAACCACCACTCTCGCCCTTACGCCACCCCCTCCCAACCCAATGCATTTGGCCCCTCCTCTTCCTACACAGAACCTCTCGATGCCAACAAGCACGCGATTGCTGTAGCTGCCGCCACCGCCGCCGTCGCCGAGGCAGCTCTAGCCGCCGCCCATGCTGCTGCCGAGGTTGTTCGGCTCACCAGCAGCGGCACCACTCACGCTAATGCTAACCGCCGTTGGATGGAGGAGGCGGCTGCCGTTAAAATCCAGTCCGCCTTTCGAGGTTATTTGGCAAGGAGGGCACTTAGGGCACTAAAGGCATTGGTAAAGCTTCAAGCTTTGGTGAGAGGTCACATTGTGAGAAAGCAAATGGCTGACATGTTAAGGCGAATGCAGACACTGGTTAGGTTGCAATCTCGAGCTTGTGCTGGTCGTTCAAACTTATCAGATTCTTTGCATTCTACCAGCAAATCCTCGCTCTCTCATATCCGTGTCCAAGCAACCCCCAATGGAAATGAACATCAAATCAGTGCTCATCATGGTAATAAATTTGACAACTCTGCACTTCTGAAGAGATGTGGTTCGAATTCAAATTTGAAGGATGTAACTGTTGTGGATAGAGCACCAGTCGGGTCGAGTTGGTTAGACCGTTGGATGGAAGAAAACTTGTGGAACGATCGAGAGTTGCCTCTAAAAAATATTCATGCTATAGATGAGAAAACAGACAAAATCCTTGAAGTAGATACATGGAAACCTCATTTCAAGTCTCAAGGAAACATCAGCAGCTTTAAAAATTCTCAAATGGCTCCAGATTTTAAAAACCACCAGAACTTTACAACGATTGACTCCCCATCAAAACACTCGTCAAAGGCTGCAAATCCAGTTTCGAGTCTTTCCTCAGGGGAAGCATCCTTGAGCTCCTTAAAATTCCCTGTTGGAAAATATGAAACAGCTCCAAGAACAGCAGAAAATAGCCCTCAAGTGCATTCTGCTTCATCCAGGCGTGGAAATGGCGCAAAGAGAGCTACCTTGTCGCCGACAAGAAGTGAGTATGCATGGGGATATTTCAGTGGCTATGCGGGTTATCCAAACTATATGGCCAACACAGAGTCATCCAAAGCAAAAGTCAGGTCACAAAGTGCCCCGAAACAGAGGGTCGAGTTAGAGAAATTTGGATCGAACAAGCGGTATGCTCAGGTATCTTGGGATGCATGGAGCTTTTCAAACAATGGTATCTCCCATGAACTCAATTCATCAAACAATGCCAATTCAGTAGCTGATCGCATGACTAAGTTTGCAAGTACCAAATCAAGATGA

Protein sequence

MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSSYTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNLSDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRAPVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQVHSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVELEKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR
Homology
BLAST of Lsi05G012530 vs. ExPASy Swiss-Prot
Match: Q9LYP2 (Protein IQ-DOMAIN 24 OS=Arabidopsis thaliana OX=3702 GN=IQD24 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.7e-56
Identity = 190/435 (43.68%), Postives = 235/435 (54.02%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGF  RLF  KK     P+          +RWSF  +  + H    ++       G    
Sbjct: 1   MGFFGRLFGSKKQEKATPN---------RRRWSF--ATRSSHPENDSSSHSSKRRGDE-- 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH-----ANANRRWMEE 120
             + L+ +KHAIAVAAATAAVAEAALAAA AAAEVVRLT+ G        + +NRRW +E
Sbjct: 61  --DVLNGDKHAIAVAAATAAVAEAALAAARAAAEVVRLTNGGRNSSVKQISRSNRRWSQE 120

Query: 121 -AAAVKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRAC 180
             AA+KIQSAFRGYLARRALRALKALVKLQALV+GHIVRKQ ADMLRRMQTLVRLQ+RA 
Sbjct: 121 YKAAMKIQSAFRGYLARRALRALKALVKLQALVKGHIVRKQTADMLRRMQTLVRLQARAR 180

Query: 181 AGR-SNLSDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKD 240
           A R S++SDS H  +      + + ++P       S H            RC S +    
Sbjct: 181 ASRSSHVSDSSHPPT------LMIPSSPQ------SFH-----------ARCVSEAEYSK 240

Query: 241 VTVVD------RAPVGSS-WLDRW-MEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPH 300
           V  +D      R+P+GSS  LD+W  EE+LW+  +         +E  DKILEVDTWKPH
Sbjct: 241 VIAMDHHHNNHRSPMGSSRLLDQWRTEESLWSAPK--------YNEDDDKILEVDTWKPH 300

Query: 301 FKSQGNISSFKNSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVG 360
           F+                          +SP K  S                        
Sbjct: 301 FR--------------------------ESPRKRGSL----------------------- 333

Query: 361 KYETAPRTAENSPQVHSASSRRGNGAKRAT-LSPTRSEYAWGYFSGYAGYPNYMANTESS 420
                P + ENSPQ+ S +     G++R T  +P RSEY   Y+SGY  +PNYMANTES 
Sbjct: 361 ---VVPTSVENSPQLRSRTGSSSGGSRRKTPFTPARSEYE--YYSGY--HPNYMANTESY 333

BLAST of Lsi05G012530 vs. ExPASy Swiss-Prot
Match: Q9FIT1 (Protein IQ-DOMAIN 23 OS=Arabidopsis thaliana OX=3702 GN=IQD23 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.6e-53
Identity = 210/493 (42.60%), Postives = 248/493 (50.30%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGF  RLF  KK            + +  +RWSF   + N   R  A  S       S  
Sbjct: 1   MGFFGRLFGSKKKSDKA------ASSRDKRRWSFTTRSSNSSKRAPAVTS------ASVV 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH-------------AN 120
               LDA+KHAIAVAAATAAVAEAAL AAHAAAEVVRLTS                    
Sbjct: 61  EQNGLDADKHAIAVAAATAAVAEAALTAAHAAAEVVRLTSGNGGRNVGGGGNSSVFQIGR 120

Query: 121 ANRRWMEE-AAAVKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTL 180
           +NRRW +E  AA+KIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQ ADMLRRMQTL
Sbjct: 121 SNRRWAQENIAAMKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQTADMLRRMQTL 180

Query: 181 VRLQSRACAGRSNLSDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCG 240
           VRLQS+A   R+  S S HS+  +S         P+ +    S H            RC 
Sbjct: 181 VRLQSQA---RARASRSSHSS--ASFHSSTALLFPSSSSSPRSLH-----------TRCV 240

Query: 241 SNSNLKDVTVVDRAPVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHF 300
           SN+   +V+ +D    GS  LD   EE+                E  DKILEVDTWKPH+
Sbjct: 241 SNA---EVSSLDHRG-GSKRLDWQAEES----------------ENGDKILEVDTWKPHY 300

Query: 301 KSQGNISSFKNSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGK 360
             +   S   N                +SP K                            
Sbjct: 301 HPKPLRSERNN----------------ESPRKRQQSL----------------------- 360

Query: 361 YETAPRTAENSPQVHSASSRRGNGAKRATLSPT-RSEYAWG----YFSGYAGYPNYMANT 420
               PR+ ENSPQV S+ SRR     R   +PT RSEY+WG    Y+SGY  +PNYMANT
Sbjct: 361 --LGPRSTENSPQVGSSGSRR-----RTPFTPTSRSEYSWGCNNYYYSGY--HPNYMANT 396

Query: 421 ESSKAKVRSQSAPKQRVELEKFGSN-KRYAQVSWDAWSFSNNGISHELNSS---NNANSV 471
           ES KAKVRSQSAPKQRVE+    S  KR  Q  +  ++      S ++ S+         
Sbjct: 421 ESYKAKVRSQSAPKQRVEVSNETSGYKRSVQGQYYYYTAVEEE-SLDVGSAGYYGGGGGD 396

BLAST of Lsi05G012530 vs. ExPASy Swiss-Prot
Match: Q2NNE0 (Protein IQ-DOMAIN 22 OS=Arabidopsis thaliana OX=3702 GN=IQD22 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.6e-35
Identity = 181/485 (37.32%), Postives = 236/485 (48.66%), Query Frame = 0

Query: 3   FLRRLFRPKKPPPDPPDF---TPDPNKKKN--KRWSFPKSAHNHHSRPY----ATPSQPN 62
           + R LF  KKP P  PD    TP  +   N  +RWSF KS     S P      TPS PN
Sbjct: 7   WFRSLFGVKKPDPGYPDLSVETPSRSTSSNLKRRWSFVKSKREKESTPINQVPHTPSLPN 66

Query: 63  AFGPSSSY----------TEPL-------DANKHAIAVAAATAAVAEAALAAAHAAAEVV 122
           +  P  S+           +P+       D++KHAIAVAAATAAVAEAA+AAA+AAA VV
Sbjct: 67  STPPPPSHHQSSPRRRRKQKPMWEDEGSEDSDKHAIAVAAATAAVAEAAVAAANAAAAVV 126

Query: 123 RLTSSG--TTHANANRRWME--------------------EAAAVKIQSAFRGYLARRAL 182
           RLTS+   +T +    R+ +                    E A +KIQS FRGYLA+RAL
Sbjct: 127 RLTSTSGRSTRSPVKARFSDGFDDVVAHGSKFYGHGRDSCELAVIKIQSIFRGYLAKRAL 186

Query: 183 RALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGR----SNLSDSLHSTSKS 242
           RALK LV+LQA+VRGHI RK+M+  LRRM  LVR Q+R  A R       S S  + +KS
Sbjct: 187 RALKGLVRLQAIVRGHIERKRMSVHLRRMHALVRAQARVRATRVIVTPESSSSQSNNTKS 246

Query: 243 SLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRAPVGSSWLDRW 302
           S        TP   EH IS+   +K  +S L KR GS ++                    
Sbjct: 247 SHFQNPGPPTPEKLEHSISS-RSSKLAHSHLFKRNGSKAS-------------------- 306

Query: 303 MEENLWNDRELPL--KNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQMAPDFKNHQ 362
                 N+R  P   +   A DE+ +KIL++D        + +ISS+        F  + 
Sbjct: 307 -----DNNRLYPAHRETFSATDEE-EKILQID--------RKHISSYTRRNRPDMF--YS 366

Query: 363 NFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQVHSASSRRG 422
           +   +D+        A P S  SS E   S              TAENSPQ++SA+SR  
Sbjct: 367 SHLILDNAGLSEPVFATPFSPSSSHEEITSQF-----------CTAENSPQLYSATSRSK 426

Query: 423 NGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL-EKFGSNK 433
             A  A+ S   S+       G   +P+YMA TESS+AK RS SAPK R +L  +  S+K
Sbjct: 427 RSAFTAS-SIAPSDCTKSCCDG--DHPSYMACTESSRAKARSASAPKSRPQLFYERPSSK 440

BLAST of Lsi05G012530 vs. ExPASy Swiss-Prot
Match: Q9LK76 (Protein IQ-domain 26 OS=Arabidopsis thaliana OX=3702 GN=IQD26 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.9e-27
Identity = 130/363 (35.81%), Postives = 173/363 (47.66%), Query Frame = 0

Query: 66  DANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTT---HANANRRWMEEAAAVKIQ 125
           + NKHAIAVAAATAA A+AA+AAA AA  VVRLTS+G +     NA  RW    AAVKIQ
Sbjct: 59  EQNKHAIAVAAATAAAADAAVAAAQAAVAVVRLTSNGRSGGYSGNAMERW----AAVKIQ 118

Query: 126 SAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNLSD 185
           S F+GYLAR+ALRALK LVKLQALVRG++VRK+ A+ L  MQ L+R Q+   + R N ++
Sbjct: 119 SVFKGYLARKALRALKGLVKLQALVRGYLVRKRAAETLHSMQALIRAQTSVRSQRINRNN 178

Query: 186 SLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRAPV 245
             H                         H   + D+S                   R+ +
Sbjct: 179 MFH-----------------------PRHSLERLDDS-------------------RSEI 238

Query: 246 GSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQMAP 305
            S  +   +E+           N +A DE + KI+E+DT+K   KS+    +   S+   
Sbjct: 239 HSKRISISVEKQ-------SNHNNNAYDETSPKIVEIDTYKT--KSRSKRMNVAVSECGD 298

Query: 306 DFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQVHS 365
           DF           P +                      KFP         TA+N+P+   
Sbjct: 299 DFIYQAKDFEWSFPGE--------------------KCKFP---------TAQNTPRF-- 335

Query: 366 ASSRRGNGAKRATLSPTRSEYAWGYF-SGYAGY--PNYMANTESSKAKVRSQSAPKQRVE 423
           +SS   N       SP +S      F   Y G   P+YMANT+S KAKVRS SAP+QR +
Sbjct: 359 SSSMANNNYYYTPPSPAKSVCRDACFRPSYPGLMTPSYMANTQSFKAKVRSHSAPRQRPD 335

BLAST of Lsi05G012530 vs. ExPASy Swiss-Prot
Match: F4IUJ7 (Protein IQ-DOMAIN 4 OS=Arabidopsis thaliana OX=3702 GN=IQD4 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.4e-18
Identity = 126/445 (28.31%), Postives = 198/445 (44.49%), Query Frame = 0

Query: 9   RPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSSYTEPLDAN 68
           RP  PPP  PDF P P          P         P + P  P A+  ++        +
Sbjct: 62  RPYPPPPPLPDFAPQP--------LLP---------PPSPPPPPPAYTINTRIYGESKES 121

Query: 69  KHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVKIQSAFRGY 128
           K+  A+A A+A  AEAA+ AAHAAAEV+RLT+  T     ++   EE AA+KIQ+A+R Y
Sbjct: 122 KNRQALALASAVAAEAAVVAAHAAAEVIRLTTPSTPQIEESK---EETAAIKIQNAYRCY 181

Query: 129 LARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNLSDS----- 188
            ARR LRAL+ + +L++L++G  V++QM  ML  MQTL RLQ++    R+ LS       
Sbjct: 182 TARRTLRALRGMARLKSLLQGKYVKRQMNAMLSSMQTLTRLQTQIQERRNRLSAENKTRH 241

Query: 189 --LHSTSKSSLSHIRVQA-----TPNGNEHQISAHHGNKFDNSALLKRC----------- 248
             +        +H  +       + N ++ QI A   N+ + S   +R            
Sbjct: 242 RLIQQKGHQKENHQNLVTAGNFDSSNKSKEQIVARSVNRKEASVRRERALAYAYSHQQTW 301

Query: 249 GSNSNLKDVTVVDRAPV--GSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWK 308
            ++S L   T++D      G SWL+RWM    W+   +  +       K +  ++    +
Sbjct: 302 RNSSKLPHQTLMDTNTTDWGWSWLERWMASRPWDAESIDDQVSVKSSLKRENSIKSSPAR 361

Query: 309 PHFKSQGNISSFK----NSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSS 368
              +   + SS +    N   +   +      +I   S  ++K    V S SS   SL +
Sbjct: 362 SKTQKSASQSSIQWPVNNDTKSRKIEVTNRRHSIGGGSSENAKDDESVGSSSSRRNSLDN 421

Query: 369 LKFPVGKYETAPRTAENSPQVHSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMA 423
            +    K + +  T  N     +   +   GAKR  L  T++  +        G    +A
Sbjct: 422 TQ--TVKSKVSVETTSNVSNAQTVKPKANVGAKR-NLDNTKTLKS----KSSVGTTGNLA 479

BLAST of Lsi05G012530 vs. ExPASy TrEMBL
Match: A0A0A0L9P9 (DUF4005 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G180400 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.2e-238
Identity = 439/470 (93.40%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRP KPPP  PD TP PN KKNKRWSF KS HNHHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPNKPPPALPDSTPGPNIKKNKRWSFGKSGHNHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHA+AVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAMAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATPNG   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVDRA
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPNGTGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDRA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           APDF+NHQ+F TIDSPSKHSSKAANPVSSLSSGE SLSSLKFPVGKYE APRTAENSPQV
Sbjct: 301 APDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEVSLSSLKFPVGKYEQAPRTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNTAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHE NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHEPNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. ExPASy TrEMBL
Match: A0A5D3DGA9 (Protein IQ-DOMAIN 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00450 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.6e-237
Identity = 438/470 (93.19%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPPP+ PD TP PN  KNKRWSF KS H HHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPPNLPDSTPHPNNNKNKRWSFGKSGHTHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATP+G   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPSGIGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           A DF+NHQ+F TIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYE APRTAENSPQV
Sbjct: 301 ASDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYEQAPRTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNAAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. ExPASy TrEMBL
Match: A0A5A7SW19 (Protein IQ-DOMAIN 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G00570 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.9e-235
Identity = 436/470 (92.77%), Postives = 448/470 (95.32%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPPP+ PD TP PN  KNKRWSF KS H HHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPPNLPDSTPHPNNNKNKRWSFGKSGHTHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATP+G   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPSGIGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           A DF+NHQ+F TIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYE AP TAENSPQV
Sbjct: 301 ASDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYEQAPGTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNAAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHE NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHEPNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. ExPASy TrEMBL
Match: A0A1S3AYT6 (protein IQ-DOMAIN 14 OS=Cucumis melo OX=3656 GN=LOC103484053 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.9e-235
Identity = 436/470 (92.77%), Postives = 448/470 (95.32%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPPP+ PD TP PN  KNKRWSF KS H HHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPPNLPDSTPHPNNNKNKRWSFGKSGHTHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATP+G   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPSGIGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           A DF+NHQ+F TIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYE AP TAENSPQV
Sbjct: 301 ASDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYEQAPGTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNAAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHE NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHEPNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. ExPASy TrEMBL
Match: A0A6J1J2R1 (protein IQ-DOMAIN 14-like OS=Cucurbita maxima OX=3661 GN=LOC111482867 PE=4 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.8e-226
Identity = 425/470 (90.43%), Postives = 442/470 (94.04%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPP DPPD T   NKKKN+RW F KSAHN  SRPY T SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPLDPPDSTQGLNKKKNRRWGFAKSAHNDQSRPYTTQSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSS TTHANANRRWMEEAAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSSTTHANANRRWMEEAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALV+LQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVRLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATPNGN+HQ  AH  NKFDNSALLKRCGSNSNLKDV+VVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPNGNDHQFCAHLSNKFDNSALLKRCGSNSNLKDVSVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+L LKNIHA+DEKTDKILEV+TWKPH KSQGNISSFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRDLALKNIHAVDEKTDKILEVETWKPHLKSQGNISSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           APDFKN Q+FTTIDSPSKHSSKAA PVSSLSSGEASLSSLK+P+GKYE APRT ENSPQV
Sbjct: 301 APDFKN-QSFTTIDSPSKHSSKAAKPVSSLSSGEASLSSLKYPLGKYEAAPRTGENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSA+SRRGNGAKRAT SPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAP+QR+EL
Sbjct: 361 HSANSRRGNGAKRATFSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPRQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EK GSNKR++QVSWDAWSFSN+GI  E NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKIGSNKRHSQVSWDAWSFSNSGI--EPNSSNNANSVADRMTKFASTKSR 467

BLAST of Lsi05G012530 vs. NCBI nr
Match: XP_038892577.1 (protein IQ-DOMAIN 14 [Benincasa hispida])

HSP 1 Score: 849.0 bits (2192), Expect = 2.0e-242
Identity = 445/470 (94.68%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPPPDPPD T DP+KKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPPDPPDSTSDPSKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH NANRRWMEE AAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHTNANRRWMEETAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLS+IRVQ+ PN NEHQ+ AHH NK+DNSALLKRCGSNSNLKDVTVVDRA
Sbjct: 181 SDSLHSTSKSSLSNIRVQSIPNRNEHQLCAHHSNKYDNSALLKRCGSNSNLKDVTVVDRA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIH +DEKTDKILEVDTWKPH KSQGNISSFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRDLPLKNIHDVDEKTDKILEVDTWKPHLKSQGNISSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           APDFKNHQ+FTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV
Sbjct: 301 APDFKNHQSFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGNGAKRATLSPTRSEYAW YFSGYA YPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNGAKRATLSPTRSEYAWRYFSGYAAYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHE NSSNNANSVADR+ KFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHEPNSSNNANSVADRIDKFASTKSR 470

BLAST of Lsi05G012530 vs. NCBI nr
Match: XP_004140851.3 (protein IQ-DOMAIN 14 [Cucumis sativus] >KAE8650451.1 hypothetical protein Csa_010561 [Cucumis sativus])

HSP 1 Score: 833.9 bits (2153), Expect = 6.6e-238
Identity = 440/470 (93.62%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRP KPPP  PD TP PN KKNKRWSF KS HNHHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPNKPPPALPDSTPGPNIKKNKRWSFGKSGHNHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATPNG   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVDRA
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPNGTGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDRA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           APDF+NHQ+F TIDSPSKHSSKAANPVSSLSSGE SLSSLKFPVGKYE APRTAENSPQV
Sbjct: 301 APDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEVSLSSLKFPVGKYEQAPRTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNTAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHE NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHEPNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. NCBI nr
Match: TYK22289.1 (protein IQ-DOMAIN 14 [Cucumis melo var. makuwa])

HSP 1 Score: 830.1 bits (2143), Expect = 9.6e-237
Identity = 438/470 (93.19%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPPP+ PD TP PN  KNKRWSF KS H HHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPPNLPDSTPHPNNNKNKRWSFGKSGHTHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATP+G   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPSGIGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           A DF+NHQ+F TIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYE APRTAENSPQV
Sbjct: 301 ASDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYEQAPRTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNAAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. NCBI nr
Match: XP_008439181.1 (PREDICTED: protein IQ-DOMAIN 14 [Cucumis melo] >KAA0033621.1 protein IQ-DOMAIN 14 [Cucumis melo var. makuwa])

HSP 1 Score: 824.7 bits (2129), Expect = 4.0e-235
Identity = 436/470 (92.77%), Postives = 448/470 (95.32%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPPP+ PD TP PN  KNKRWSF KS H HHSRPYAT SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPPNLPDSTPHPNNNKNKRWSFGKSGHTHHSRPYATSSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH+NANRRWME+AAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHSNANRRWMEDAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATP+G   Q+ AHH NKFDNSALLKRCGSNSNLKDVTVVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPSGIGDQLCAHHSNKFDNSALLKRCGSNSNLKDVTVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+LPLKNIHA+DEKTDKILEVDTWKPH KSQGNI+SFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRQLPLKNIHAVDEKTDKILEVDTWKPHLKSQGNINSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           A DF+NHQ+F TIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYE AP TAENSPQV
Sbjct: 301 ASDFRNHQSFMTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYEQAPGTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSASSRRGN AKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQR+EL
Sbjct: 361 HSASSRRGNAAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EKFGSNKRYAQVSWDAWSFSNNGISHE NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKFGSNKRYAQVSWDAWSFSNNGISHEPNSSNNANSVADRMTKFASTKSR 470

BLAST of Lsi05G012530 vs. NCBI nr
Match: XP_023552437.1 (protein IQ-DOMAIN 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.4 bits (2071), Expect = 2.1e-228
Identity = 428/470 (91.06%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGFLRRLFRPKKPP DPPD T   NKKKN+RW F KSAHN  SRPY T SQPNAFGPSSS
Sbjct: 1   MGFLRRLFRPKKPPLDPPDSTQGLNKKKNRRWGFAKSAHNDQSRPYTTQSQPNAFGPSSS 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVK 120
           YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSS TTHANANRRWMEEAAAVK
Sbjct: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSTTTHANANRRWMEEAAAVK 120

Query: 121 IQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180
           IQSAFRGYLARRALRALKALV+LQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL
Sbjct: 121 IQSAFRGYLARRALRALKALVRLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNL 180

Query: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRA 240
           SDSLHSTSKSSLSHIRVQATPNGNEHQ  AH  NKFDNSALLKRCGSNSNLKDV+VVD A
Sbjct: 181 SDSLHSTSKSSLSHIRVQATPNGNEHQFCAHLSNKFDNSALLKRCGSNSNLKDVSVVDGA 240

Query: 241 PVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQM 300
           PVGSSWLDRWMEENLWN+R+L LKNIHA+DEKTDKILEVDTWKPH KSQGNISSFKNSQM
Sbjct: 241 PVGSSWLDRWMEENLWNNRDLALKNIHAVDEKTDKILEVDTWKPHLKSQGNISSFKNSQM 300

Query: 301 APDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQV 360
           AP+FKN Q+FTTIDSPSKHSSKAA PVSSLSSGEASLSSLK+P+GKYE A RTAENSPQV
Sbjct: 301 APEFKN-QSFTTIDSPSKHSSKAAKPVSSLSSGEASLSSLKYPLGKYEAATRTAENSPQV 360

Query: 361 HSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL 420
           HSA+SRRGNGAKRAT SPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAP+QR+EL
Sbjct: 361 HSANSRRGNGAKRATFSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPRQRLEL 420

Query: 421 EKFGSNKRYAQVSWDAWSFSNNGISHELNSSNNANSVADRMTKFASTKSR 471
           EK GSNKR++QVSWDAWSFSN+GISHE NSSNNANSVADRMTKFASTKSR
Sbjct: 421 EKLGSNKRHSQVSWDAWSFSNSGISHEPNSSNNANSVADRMTKFASTKSR 469

BLAST of Lsi05G012530 vs. TAIR 10
Match: AT5G07240.1 (IQ-domain 24 )

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-57
Identity = 190/435 (43.68%), Postives = 235/435 (54.02%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGF  RLF  KK     P+          +RWSF  +  + H    ++       G    
Sbjct: 1   MGFFGRLFGSKKQEKATPN---------RRRWSF--ATRSSHPENDSSSHSSKRRGDE-- 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH-----ANANRRWMEE 120
             + L+ +KHAIAVAAATAAVAEAALAAA AAAEVVRLT+ G        + +NRRW +E
Sbjct: 61  --DVLNGDKHAIAVAAATAAVAEAALAAARAAAEVVRLTNGGRNSSVKQISRSNRRWSQE 120

Query: 121 -AAAVKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRAC 180
             AA+KIQSAFRGYLARRALRALKALVKLQALV+GHIVRKQ ADMLRRMQTLVRLQ+RA 
Sbjct: 121 YKAAMKIQSAFRGYLARRALRALKALVKLQALVKGHIVRKQTADMLRRMQTLVRLQARAR 180

Query: 181 AGR-SNLSDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKD 240
           A R S++SDS H  +      + + ++P       S H            RC S +    
Sbjct: 181 ASRSSHVSDSSHPPT------LMIPSSPQ------SFH-----------ARCVSEAEYSK 240

Query: 241 VTVVD------RAPVGSS-WLDRW-MEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPH 300
           V  +D      R+P+GSS  LD+W  EE+LW+  +         +E  DKILEVDTWKPH
Sbjct: 241 VIAMDHHHNNHRSPMGSSRLLDQWRTEESLWSAPK--------YNEDDDKILEVDTWKPH 300

Query: 301 FKSQGNISSFKNSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVG 360
           F+                          +SP K  S                        
Sbjct: 301 FR--------------------------ESPRKRGSL----------------------- 333

Query: 361 KYETAPRTAENSPQVHSASSRRGNGAKRAT-LSPTRSEYAWGYFSGYAGYPNYMANTESS 420
                P + ENSPQ+ S +     G++R T  +P RSEY   Y+SGY  +PNYMANTES 
Sbjct: 361 ---VVPTSVENSPQLRSRTGSSSGGSRRKTPFTPARSEYE--YYSGY--HPNYMANTESY 333

BLAST of Lsi05G012530 vs. TAIR 10
Match: AT5G62070.1 (IQ-domain 23 )

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-54
Identity = 210/493 (42.60%), Postives = 248/493 (50.30%), Query Frame = 0

Query: 1   MGFLRRLFRPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSS 60
           MGF  RLF  KK            + +  +RWSF   + N   R  A  S       S  
Sbjct: 1   MGFFGRLFGSKKKSDKA------ASSRDKRRWSFTTRSSNSSKRAPAVTS------ASVV 60

Query: 61  YTEPLDANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTH-------------AN 120
               LDA+KHAIAVAAATAAVAEAAL AAHAAAEVVRLTS                    
Sbjct: 61  EQNGLDADKHAIAVAAATAAVAEAALTAAHAAAEVVRLTSGNGGRNVGGGGNSSVFQIGR 120

Query: 121 ANRRWMEE-AAAVKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTL 180
           +NRRW +E  AA+KIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQ ADMLRRMQTL
Sbjct: 121 SNRRWAQENIAAMKIQSAFRGYLARRALRALKALVKLQALVRGHIVRKQTADMLRRMQTL 180

Query: 181 VRLQSRACAGRSNLSDSLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCG 240
           VRLQS+A   R+  S S HS+  +S         P+ +    S H            RC 
Sbjct: 181 VRLQSQA---RARASRSSHSS--ASFHSSTALLFPSSSSSPRSLH-----------TRCV 240

Query: 241 SNSNLKDVTVVDRAPVGSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHF 300
           SN+   +V+ +D    GS  LD   EE+                E  DKILEVDTWKPH+
Sbjct: 241 SNA---EVSSLDHRG-GSKRLDWQAEES----------------ENGDKILEVDTWKPHY 300

Query: 301 KSQGNISSFKNSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGK 360
             +   S   N                +SP K                            
Sbjct: 301 HPKPLRSERNN----------------ESPRKRQQSL----------------------- 360

Query: 361 YETAPRTAENSPQVHSASSRRGNGAKRATLSPT-RSEYAWG----YFSGYAGYPNYMANT 420
               PR+ ENSPQV S+ SRR     R   +PT RSEY+WG    Y+SGY  +PNYMANT
Sbjct: 361 --LGPRSTENSPQVGSSGSRR-----RTPFTPTSRSEYSWGCNNYYYSGY--HPNYMANT 396

Query: 421 ESSKAKVRSQSAPKQRVELEKFGSN-KRYAQVSWDAWSFSNNGISHELNSS---NNANSV 471
           ES KAKVRSQSAPKQRVE+    S  KR  Q  +  ++      S ++ S+         
Sbjct: 421 ESYKAKVRSQSAPKQRVEVSNETSGYKRSVQGQYYYYTAVEEE-SLDVGSAGYYGGGGGD 396

BLAST of Lsi05G012530 vs. TAIR 10
Match: AT4G23060.1 (IQ-domain 22 )

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-36
Identity = 181/485 (37.32%), Postives = 236/485 (48.66%), Query Frame = 0

Query: 3   FLRRLFRPKKPPPDPPDF---TPDPNKKKN--KRWSFPKSAHNHHSRPY----ATPSQPN 62
           + R LF  KKP P  PD    TP  +   N  +RWSF KS     S P      TPS PN
Sbjct: 7   WFRSLFGVKKPDPGYPDLSVETPSRSTSSNLKRRWSFVKSKREKESTPINQVPHTPSLPN 66

Query: 63  AFGPSSSY----------TEPL-------DANKHAIAVAAATAAVAEAALAAAHAAAEVV 122
           +  P  S+           +P+       D++KHAIAVAAATAAVAEAA+AAA+AAA VV
Sbjct: 67  STPPPPSHHQSSPRRRRKQKPMWEDEGSEDSDKHAIAVAAATAAVAEAAVAAANAAAAVV 126

Query: 123 RLTSSG--TTHANANRRWME--------------------EAAAVKIQSAFRGYLARRAL 182
           RLTS+   +T +    R+ +                    E A +KIQS FRGYLA+RAL
Sbjct: 127 RLTSTSGRSTRSPVKARFSDGFDDVVAHGSKFYGHGRDSCELAVIKIQSIFRGYLAKRAL 186

Query: 183 RALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGR----SNLSDSLHSTSKS 242
           RALK LV+LQA+VRGHI RK+M+  LRRM  LVR Q+R  A R       S S  + +KS
Sbjct: 187 RALKGLVRLQAIVRGHIERKRMSVHLRRMHALVRAQARVRATRVIVTPESSSSQSNNTKS 246

Query: 243 SLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRAPVGSSWLDRW 302
           S        TP   EH IS+   +K  +S L KR GS ++                    
Sbjct: 247 SHFQNPGPPTPEKLEHSISS-RSSKLAHSHLFKRNGSKAS-------------------- 306

Query: 303 MEENLWNDRELPL--KNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQMAPDFKNHQ 362
                 N+R  P   +   A DE+ +KIL++D        + +ISS+        F  + 
Sbjct: 307 -----DNNRLYPAHRETFSATDEE-EKILQID--------RKHISSYTRRNRPDMF--YS 366

Query: 363 NFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQVHSASSRRG 422
           +   +D+        A P S  SS E   S              TAENSPQ++SA+SR  
Sbjct: 367 SHLILDNAGLSEPVFATPFSPSSSHEEITSQF-----------CTAENSPQLYSATSRSK 426

Query: 423 NGAKRATLSPTRSEYAWGYFSGYAGYPNYMANTESSKAKVRSQSAPKQRVEL-EKFGSNK 433
             A  A+ S   S+       G   +P+YMA TESS+AK RS SAPK R +L  +  S+K
Sbjct: 427 RSAFTAS-SIAPSDCTKSCCDG--DHPSYMACTESSRAKARSASAPKSRPQLFYERPSSK 440

BLAST of Lsi05G012530 vs. TAIR 10
Match: AT3G16490.1 (IQ-domain 26 )

HSP 1 Score: 122.9 bits (307), Expect = 7.0e-28
Identity = 130/363 (35.81%), Postives = 173/363 (47.66%), Query Frame = 0

Query: 66  DANKHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTT---HANANRRWMEEAAAVKIQ 125
           + NKHAIAVAAATAA A+AA+AAA AA  VVRLTS+G +     NA  RW    AAVKIQ
Sbjct: 59  EQNKHAIAVAAATAAAADAAVAAAQAAVAVVRLTSNGRSGGYSGNAMERW----AAVKIQ 118

Query: 126 SAFRGYLARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNLSD 185
           S F+GYLAR+ALRALK LVKLQALVRG++VRK+ A+ L  MQ L+R Q+   + R N ++
Sbjct: 119 SVFKGYLARKALRALKGLVKLQALVRGYLVRKRAAETLHSMQALIRAQTSVRSQRINRNN 178

Query: 186 SLHSTSKSSLSHIRVQATPNGNEHQISAHHGNKFDNSALLKRCGSNSNLKDVTVVDRAPV 245
             H                         H   + D+S                   R+ +
Sbjct: 179 MFH-----------------------PRHSLERLDDS-------------------RSEI 238

Query: 246 GSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWKPHFKSQGNISSFKNSQMAP 305
            S  +   +E+           N +A DE + KI+E+DT+K   KS+    +   S+   
Sbjct: 239 HSKRISISVEKQ-------SNHNNNAYDETSPKIVEIDTYKT--KSRSKRMNVAVSECGD 298

Query: 306 DFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSSLKFPVGKYETAPRTAENSPQVHS 365
           DF           P +                      KFP         TA+N+P+   
Sbjct: 299 DFIYQAKDFEWSFPGE--------------------KCKFP---------TAQNTPRF-- 335

Query: 366 ASSRRGNGAKRATLSPTRSEYAWGYF-SGYAGY--PNYMANTESSKAKVRSQSAPKQRVE 423
           +SS   N       SP +S      F   Y G   P+YMANT+S KAKVRS SAP+QR +
Sbjct: 359 SSSMANNNYYYTPPSPAKSVCRDACFRPSYPGLMTPSYMANTQSFKAKVRSHSAPRQRPD 335

BLAST of Lsi05G012530 vs. TAIR 10
Match: AT2G26410.1 (IQ-domain 4 )

HSP 1 Score: 93.6 bits (231), Expect = 4.6e-19
Identity = 126/445 (28.31%), Postives = 198/445 (44.49%), Query Frame = 0

Query: 9   RPKKPPPDPPDFTPDPNKKKNKRWSFPKSAHNHHSRPYATPSQPNAFGPSSSYTEPLDAN 68
           RP  PPP  PDF P P          P         P + P  P A+  ++        +
Sbjct: 62  RPYPPPPPLPDFAPQP--------LLP---------PPSPPPPPPAYTINTRIYGESKES 121

Query: 69  KHAIAVAAATAAVAEAALAAAHAAAEVVRLTSSGTTHANANRRWMEEAAAVKIQSAFRGY 128
           K+  A+A A+A  AEAA+ AAHAAAEV+RLT+  T     ++   EE AA+KIQ+A+R Y
Sbjct: 122 KNRQALALASAVAAEAAVVAAHAAAEVIRLTTPSTPQIEESK---EETAAIKIQNAYRCY 181

Query: 129 LARRALRALKALVKLQALVRGHIVRKQMADMLRRMQTLVRLQSRACAGRSNLSDS----- 188
            ARR LRAL+ + +L++L++G  V++QM  ML  MQTL RLQ++    R+ LS       
Sbjct: 182 TARRTLRALRGMARLKSLLQGKYVKRQMNAMLSSMQTLTRLQTQIQERRNRLSAENKTRH 241

Query: 189 --LHSTSKSSLSHIRVQA-----TPNGNEHQISAHHGNKFDNSALLKRC----------- 248
             +        +H  +       + N ++ QI A   N+ + S   +R            
Sbjct: 242 RLIQQKGHQKENHQNLVTAGNFDSSNKSKEQIVARSVNRKEASVRRERALAYAYSHQQTW 301

Query: 249 GSNSNLKDVTVVDRAPV--GSSWLDRWMEENLWNDRELPLKNIHAIDEKTDKILEVDTWK 308
            ++S L   T++D      G SWL+RWM    W+   +  +       K +  ++    +
Sbjct: 302 RNSSKLPHQTLMDTNTTDWGWSWLERWMASRPWDAESIDDQVSVKSSLKRENSIKSSPAR 361

Query: 309 PHFKSQGNISSFK----NSQMAPDFKNHQNFTTIDSPSKHSSKAANPVSSLSSGEASLSS 368
              +   + SS +    N   +   +      +I   S  ++K    V S SS   SL +
Sbjct: 362 SKTQKSASQSSIQWPVNNDTKSRKIEVTNRRHSIGGGSSENAKDDESVGSSSSRRNSLDN 421

Query: 369 LKFPVGKYETAPRTAENSPQVHSASSRRGNGAKRATLSPTRSEYAWGYFSGYAGYPNYMA 423
            +    K + +  T  N     +   +   GAKR  L  T++  +        G    +A
Sbjct: 422 TQ--TVKSKVSVETTSNVSNAQTVKPKANVGAKR-NLDNTKTLKS----KSSVGTTGNLA 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYP22.7e-5643.68Protein IQ-DOMAIN 24 OS=Arabidopsis thaliana OX=3702 GN=IQD24 PE=1 SV=1[more]
Q9FIT11.6e-5342.60Protein IQ-DOMAIN 23 OS=Arabidopsis thaliana OX=3702 GN=IQD23 PE=1 SV=1[more]
Q2NNE02.6e-3537.32Protein IQ-DOMAIN 22 OS=Arabidopsis thaliana OX=3702 GN=IQD22 PE=1 SV=1[more]
Q9LK769.9e-2735.81Protein IQ-domain 26 OS=Arabidopsis thaliana OX=3702 GN=IQD26 PE=1 SV=1[more]
F4IUJ76.4e-1828.31Protein IQ-DOMAIN 4 OS=Arabidopsis thaliana OX=3702 GN=IQD4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9P97.2e-23893.40DUF4005 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G180400 PE=... [more]
A0A5D3DGA94.6e-23793.19Protein IQ-DOMAIN 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440... [more]
A0A5A7SW191.9e-23592.77Protein IQ-DOMAIN 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239... [more]
A0A1S3AYT61.9e-23592.77protein IQ-DOMAIN 14 OS=Cucumis melo OX=3656 GN=LOC103484053 PE=4 SV=1[more]
A0A6J1J2R12.8e-22690.43protein IQ-DOMAIN 14-like OS=Cucurbita maxima OX=3661 GN=LOC111482867 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038892577.12.0e-24294.68protein IQ-DOMAIN 14 [Benincasa hispida][more]
XP_004140851.36.6e-23893.62protein IQ-DOMAIN 14 [Cucumis sativus] >KAE8650451.1 hypothetical protein Csa_01... [more]
TYK22289.19.6e-23793.19protein IQ-DOMAIN 14 [Cucumis melo var. makuwa][more]
XP_008439181.14.0e-23592.77PREDICTED: protein IQ-DOMAIN 14 [Cucumis melo] >KAA0033621.1 protein IQ-DOMAIN 1... [more]
XP_023552437.12.1e-22891.06protein IQ-DOMAIN 14-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G07240.11.9e-5743.68IQ-domain 24 [more]
AT5G62070.11.2e-5442.60IQ-domain 23 [more]
AT4G23060.11.8e-3637.32IQ-domain 22 [more]
AT3G16490.17.0e-2835.81IQ-domain 26 [more]
AT2G26410.14.6e-1928.31IQ-domain 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000048IQ motif, EF-hand binding siteSMARTSM00015iq_5coord: 113..135
e-value: 4.3E-6
score: 36.2
coord: 136..157
e-value: 21.0
score: 11.1
IPR000048IQ motif, EF-hand binding sitePFAMPF00612IQcoord: 116..135
e-value: 7.6E-7
score: 28.4
coord: 138..155
e-value: 0.01
score: 15.6
IPR000048IQ motif, EF-hand binding sitePROSITEPS50096IQcoord: 114..142
score: 11.3899
IPR000048IQ motif, EF-hand binding sitePROSITEPS50096IQcoord: 138..165
score: 10.1821
IPR025064Domain of unknown function DUF4005PFAMPF13178DUF4005coord: 357..440
e-value: 4.2E-13
score: 49.8
NoneNo IPR availableGENE3D1.20.5.190coord: 113..166
e-value: 1.5E-11
score: 46.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 450..470
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 348..376
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..333
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 352..376
NoneNo IPR availablePANTHERPTHR32295:SF174CALMODULIN-BINDING PROTEINcoord: 1..468
NoneNo IPR availablePANTHERPTHR32295IQ-DOMAIN 5-RELATEDcoord: 1..468
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 105..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G012530.1Lsi05G012530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0005515 protein binding