Lsi05G011520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G011520
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	ATP-dependent DNA helicase
Location	chr05: 19376498 .. 19378585 (-)
RNA-Seq Expression	Lsi05G011520
Synteny	Lsi05G011520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTCTGACCTAAGGAAAGAGGAAATAGAGCAACTTATCATACAACTTTTATTAGATCGTGTTTTGGTAAGGATTCTTCCTCTTTATGTAACTTGCTTTATACATTGAAGGCCTAGGGGAAGGTGGGGGGAGGTGGAACCATGGAGGAATTTAGCATTTTATTGCATCCTTGAGGTTCTCTCAAATAAAATATTTTTCTCCTGCCTTTTCCTAATTGAGAGAAATAAGGAGCATTGATGTAACCTCTTTACGTTCTATTGGGATTGGAGACCTTCTGTATTGATTTTATTATATATACACTTGTGCTCTAAAACGTTGCTTGGGTCCCCTCCAAAGCTAAAATATAGAATCAAACTTATGTCACGTAACTCACAGTATGAAACATTCTAATTGTTCTTTAAGATTAATACCCAATGCTTCTCGGTCAGCATTCAATCAAGAACTCTATAGCATCATAGGAAGTAGAAATTCTCTTCTATTCTTTTGATCGGAAGTTTTCTTCTATTTATCAGTCTAATTAATTACTATGACATGTAGTAAGTTCTTCCTGCTCGTTGATTGACTTACCTATGCTGCTATATTATTATCTCTTCTAATCCTTTGTGACAATGAAACAATTTTCTGTTGCTTCAGAAAGAAGAATTTCAGCATACAGCTTATGCTACGAATGCGTACGTTACAGTTGGGCCATTGGCCCGGCAAGTCTTGCAAGGTTAGGATGCTTTTTTCCAACAAATGATGGCCATATGTAAACACTGAGAATTAAAAGGATTATTTTCCCTTGTTATTGCTCTAAAGCCTGAAGTAGAAATGATTTTTCCGTTCTTTCAAGTTTCAACATACAAAAATTAAGGAAAACCACCGTACCTTAATTTTCAGTTGTTATATCTATCCTCAAAAGTCTCTCTAGTCCAATGACCTGATAAAAAAAAATTTGAACTTGGACGCCTCCAATCACATAATTTTGAATTTCAGAGAATCAGAAACACAATTTGGTTATCGAACTATAATGATTCCTACTTTTCTTGAAAAAATGTGAAACTGGAAAAGGATTACAAGAAGTATAATAGTTGACTTTGTTGCCATTGTTTTGCATACAGGGAAGAAGGTCGTGAAACTTGAGATTTCCGGCAAACAGAAGGTAACTGGTGTTAAATCTAGAAGAAAAAGTGTTGCTTCATCAGGTTTGGGGTATAAGCTTGATGAGCTACGCAAGGAACTTGCGTCCGTTGACGGAGGCATATTCCCTCATTCAGTGCTCTCGACCCAGCAAATCGGTCTCTTAAGTGCCCAAAAACCCACTTCCTTGGATCAGGCAAGCTTCTTACTATCAATTTTAAGTACTGAGTTCGAAAACGGTGGCTGCTGGTTGATTAGGAAGCCTTTATATACAGATGTCCTGTTCAATTAAATTATAAGCAACTGAATTTAAGGATTGCAAAAAGTTCCCACTGGCTGAGATCAATTAGGCCCATTTTGATAACTATTTTGTTTTTAGTTTCCTGCTTTATTTTGAAAACTATGCTTCTTTCCATGTAATTTTTTTATAAATTTTTTTCTTACTTAAAAACACATTTAAATTTTAAGCCAAATTCAAAAAACATAATCAAATTTTTTAAAATACTTTTTTTTTTAGTTTTTAAAATTTGGCTTGGTTAGTGAAACATTTTTGAAAAGTAGGTAATAAATTATGAAAACTACAAATAAGTAGTGTTTATAAGCTCAATTCTGTTAAAGTTGTAGAAAGTGTTTGTCTGAGTTTAAGGTCTTAAAAATTCAACTTGTTTCTTGTTTTAAAAAAAATAGAAAGCCAACTAGTTTCTTTTATCACATTAGACTGCAGTTGTCATTCACCACTCCAAAATTCAGATAATGTTAAATGGCTTTAATTTGCAGTTGGAAACAATGATTGGAAAAGTGAAGGCAGAGAAGTACGGGAATAGAATTCTTGAAGAAATAGAGAAGTGGTCTATTGGTCAAAACCAAAGTGCTTCCCAAGAAGAGCAAGAAGATGAAAATAGGACTCCAAAAAGACCAAAAACCATCAACTCTCACGTTGTTATTGAGAGCAGCGATGAAGAAGCATGA

mRNA sequence

GTTTCTGACCTAAGGAAAGAGGAAATAGAGCAACTTATCATACAACTTTTATTAGATCGTGTTTTGAAAGAAGAATTTCAGCATACAGCTTATGCTACGAATGCGTACGTTACAGTTGGGCCATTGGCCCGGCAAGTCTTGCAAGGGAAGAAGGTCGTGAAACTTGAGATTTCCGGCAAACAGAAGGTAACTGGTGTTAAATCTAGAAGAAAAAGTGTTGCTTCATCAGGTTTGGGGTATAAGCTTGATGAGCTACGCAAGGAACTTGCGTCCGTTGACGGAGGCATATTCCCTCATTCAGTGCTCTCGACCCAGCAAATCGGTCTCTTAAGTGCCCAAAAACCCACTTCCTTGGATCAGGCAAGCTTCTTACTATCAATTTTAAGTACTGAGTTCGAAAACGGTGGCTGCTGGTTGATTAGGAAGCCTTTATATACAGATTTGGAAACAATGATTGGAAAAGTGAAGGCAGAGAAGTACGGGAATAGAATTCTTGAAGAAATAGAGAAGTGGTCTATTGGTCAAAACCAAAGTGCTTCCCAAGAAGAGCAAGAAGATGAAAATAGGACTCCAAAAAGACCAAAAACCATCAACTCTCACGTTGTTATTGAGAGCAGCGATGAAGAAGCATGA

Coding sequence (CDS)

GTTTCTGACCTAAGGAAAGAGGAAATAGAGCAACTTATCATACAACTTTTATTAGATCGTGTTTTGAAAGAAGAATTTCAGCATACAGCTTATGCTACGAATGCGTACGTTACAGTTGGGCCATTGGCCCGGCAAGTCTTGCAAGGGAAGAAGGTCGTGAAACTTGAGATTTCCGGCAAACAGAAGGTAACTGGTGTTAAATCTAGAAGAAAAAGTGTTGCTTCATCAGGTTTGGGGTATAAGCTTGATGAGCTACGCAAGGAACTTGCGTCCGTTGACGGAGGCATATTCCCTCATTCAGTGCTCTCGACCCAGCAAATCGGTCTCTTAAGTGCCCAAAAACCCACTTCCTTGGATCAGGCAAGCTTCTTACTATCAATTTTAAGTACTGAGTTCGAAAACGGTGGCTGCTGGTTGATTAGGAAGCCTTTATATACAGATTTGGAAACAATGATTGGAAAAGTGAAGGCAGAGAAGTACGGGAATAGAATTCTTGAAGAAATAGAGAAGTGGTCTATTGGTCAAAACCAAAGTGCTTCCCAAGAAGAGCAAGAAGATGAAAATAGGACTCCAAAAAGACCAAAAACCATCAACTCTCACGTTGTTATTGAGAGCAGCGATGAAGAAGCATGA

Protein sequence

VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGKQKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSASQEEQEDENRTPKRPKTINSHVVIESSDEEA

Homology

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BLAST of Lsi05G011520 vs. ExPASy Swiss-Prot
Match: Q9FT73 (ATP-dependent DNA helicase Q-like 2 OS=Arabidopsis thaliana OX=3702 GN=RECQL2 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.5e-40
Identity = 96/211 (45.50%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           +++L+++E+E L+I+L++D VLKEEFQHT Y+TNAYVT+GPLA Q+LQG+K +K+E S +
Sbjct: 524 IAELKRDEVEHLVIKLIVDSVLKEEFQHTPYSTNAYVTMGPLANQLLQGRKTIKMETSSR 583

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           Q     K  ++S+  SGL  KLDELRKE+++ DG I PH+VLSTQQIG +S+QKP SL  
Sbjct: 584 Q----TKKLKRSITFSGLELKLDELRKEISAADGSILPHTVLSTQQIGSISSQKPVSLQ- 643

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                     +LE++IGK+K EKYG+RILEE+ +      Q   
Sbjct: 644 --------------------------ELESIIGKLKTEKYGDRILEEVMRHEAVSEQLVE 703

Query: 181 QEEQED--ENRTPKRPKTINSHVVIESSDEE 210
              +E+  ++R  KR KT    V++ESS EE
Sbjct: 704 DPTKEETCKSRLRKRAKTQKDVVLVESSGEE 703

[Close]

BLAST of Lsi05G011520 vs. ExPASy TrEMBL
Match: A0A6J1HTR5 (ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111466807 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.4e-76
Identity = 164/210 (78.10%), Postives = 176/210 (83.81%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEISGK
Sbjct: 524 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISGK 583

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL Y+LDELRKELAS+DG IFPH+VLSTQQIG+LSAQKPTSLDQ
Sbjct: 584 QKITGVKSRRKSVASSGLEYRLDELRKELASIDGDIFPHAVLSTQQIGVLSAQKPTSLDQ 643

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 644 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 703

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEEQ DE+R PKRPKTI++HVVIESSDEEA
Sbjct: 704 QEEQGDEDRAPKRPKTIDTHVVIESSDEEA 706

[Close]

BLAST of Lsi05G011520 vs. ExPASy TrEMBL
Match: A0A6J1HRC8 (DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111466807 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.4e-76
Identity = 164/210 (78.10%), Postives = 176/210 (83.81%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEISGK
Sbjct: 502 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISGK 561

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL Y+LDELRKELAS+DG IFPH+VLSTQQIG+LSAQKPTSLDQ
Sbjct: 562 QKITGVKSRRKSVASSGLEYRLDELRKELASIDGDIFPHAVLSTQQIGVLSAQKPTSLDQ 621

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 622 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 681

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEEQ DE+R PKRPKTI++HVVIESSDEEA
Sbjct: 682 QEEQGDEDRAPKRPKTIDTHVVIESSDEEA 684

[Close]

BLAST of Lsi05G011520 vs. ExPASy TrEMBL
Match: A0A6J1HQ66 (ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111466807 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.4e-76
Identity = 164/210 (78.10%), Postives = 176/210 (83.81%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEISGK
Sbjct: 423 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISGK 482

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL Y+LDELRKELAS+DG IFPH+VLSTQQIG+LSAQKPTSLDQ
Sbjct: 483 QKITGVKSRRKSVASSGLEYRLDELRKELASIDGDIFPHAVLSTQQIGVLSAQKPTSLDQ 542

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 543 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 602

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEEQ DE+R PKRPKTI++HVVIESSDEEA
Sbjct: 603 QEEQGDEDRAPKRPKTIDTHVVIESSDEEA 605

[Close]

BLAST of Lsi05G011520 vs. ExPASy TrEMBL
Match: A0A0A0L974 (ATP-dependent DNA helicase OS=Cucumis sativus OX=3659 GN=Csa_3G636420 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.4e-76
Identity = 168/211 (79.62%), Postives = 178/211 (84.36%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVK+EISGK
Sbjct: 525 VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKIEISGK 584

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QKVTGVKS+RKSVA SGL  KLD+LRKELAS+DGGIFPHSVLSTQQI +LSAQKPTSLDQ
Sbjct: 585 QKVTGVKSKRKSVAPSGLECKLDKLRKELASIDGGIFPHSVLSTQQIVVLSAQKPTSLDQ 644

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYG+RILEEIEKWSIGQN +AS
Sbjct: 645 ---------------------------LETMIGKVKAEKYGDRILEEIEKWSIGQNPNAS 704

Query: 181 QEEQ-EDENRTPKRPKTINSHVVIESSDEEA 211
           +EEQ EDENRTPKRPKTI++HVVIESSDEEA
Sbjct: 705 EEEQEEDENRTPKRPKTIDTHVVIESSDEEA 708

[Close]

BLAST of Lsi05G011520 vs. ExPASy TrEMBL
Match: A0A6J1HGH1 (DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111464077 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.4e-76
Identity = 165/210 (78.57%), Postives = 175/210 (83.33%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEIS K
Sbjct: 502 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISSK 561

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL YKLDELRKELAS+DG IFPHSVLSTQQIG+LSAQKPTSLDQ
Sbjct: 562 QKITGVKSRRKSVASSGLEYKLDELRKELASIDGDIFPHSVLSTQQIGVLSAQKPTSLDQ 621

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 622 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 681

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEEQ DE+R PKRPKTI++HVVIESSDEEA
Sbjct: 682 QEEQGDEDRAPKRPKTIDTHVVIESSDEEA 684

[Close]

BLAST of Lsi05G011520 vs. NCBI nr
Match: XP_038887820.1 (LOW QUALITY PROTEIN: ATP-dependent DNA helicase Q-like 2 [Benincasa hispida])

HSP 1 Score: 308.1 bits (788), Expect = 5.7e-80
Identity = 172/210 (81.90%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK
Sbjct: 525 VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 584

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QKVTGVKSRRK VASSGL YKLDELRKELASVDGGIFPHSVLSTQQI +LSAQKPTSLDQ
Sbjct: 585 QKVTGVKSRRKGVASSGLEYKLDELRKELASVDGGIFPHSVLSTQQISVLSAQKPTSLDQ 644

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LET+IGKVKAEKYGNRILEEIEKWS+GQNQ+AS
Sbjct: 645 ---------------------------LETLIGKVKAEKYGNRILEEIEKWSVGQNQNAS 704

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEEQEDE RTPKRPKTINS +VIESSDE+A
Sbjct: 705 QEEQEDEKRTPKRPKTINSPIVIESSDEKA 707

[Close]

BLAST of Lsi05G011520 vs. NCBI nr
Match: KAG6571606.1 (ATP-dependent DNA helicase Q-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 302.8 bits (774), Expect = 2.4e-78
Identity = 168/210 (80.00%), Postives = 179/210 (85.24%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEIS K
Sbjct: 524 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISSK 583

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSV SSGL YKLDELRKELAS+DG IFPHSVLSTQQIG+LSAQKPTSLDQ
Sbjct: 584 QKITGVKSRRKSVDSSGLEYKLDELRKELASIDGDIFPHSVLSTQQIGVLSAQKPTSLDQ 643

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
           ASFL                      +LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 644 ASFL----------------------NLETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 703

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEEQ DE+R PKRPKTI++HVVIESSDEEA
Sbjct: 704 QEEQGDEDRAPKRPKTIDTHVVIESSDEEA 711

[Close]

BLAST of Lsi05G011520 vs. NCBI nr
Match: XP_023511413.1 (mediator of RNA polymerase II transcription subunit 34 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 4.5e-77
Identity = 166/210 (79.05%), Postives = 176/210 (83.81%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEISGK
Sbjct: 502 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISGK 561

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL YKLDELRKELAS+DG IFPHSVLSTQQIG+LSAQKPTSLDQ
Sbjct: 562 QKITGVKSRRKSVASSGLEYKLDELRKELASIDGDIFPHSVLSTQQIGVLSAQKPTSLDQ 621

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 622 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 681

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEE+ DENR PKRPKTI++HVVIESSDEEA
Sbjct: 682 QEEKGDENRAPKRPKTIDTHVVIESSDEEA 684

[Close]

BLAST of Lsi05G011520 vs. NCBI nr
Match: XP_023511414.1 (mediator of RNA polymerase II transcription subunit 34 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 4.5e-77
Identity = 166/210 (79.05%), Postives = 176/210 (83.81%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEISGK
Sbjct: 423 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISGK 482

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL YKLDELRKELAS+DG IFPHSVLSTQQIG+LSAQKPTSLDQ
Sbjct: 483 QKITGVKSRRKSVASSGLEYKLDELRKELASIDGDIFPHSVLSTQQIGVLSAQKPTSLDQ 542

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 543 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 602

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEE+ DENR PKRPKTI++HVVIESSDEEA
Sbjct: 603 QEEKGDENRAPKRPKTIDTHVVIESSDEEA 605

[Close]

BLAST of Lsi05G011520 vs. NCBI nr
Match: XP_023511412.1 (mediator of RNA polymerase II transcription subunit 34 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 4.5e-77
Identity = 166/210 (79.05%), Postives = 176/210 (83.81%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           VSDLRKEE+EQLIIQLLLDRVLKEEFQHTAYATNAY+TVGPLARQVLQGKKVVKLEISGK
Sbjct: 524 VSDLRKEEMEQLIIQLLLDRVLKEEFQHTAYATNAYITVGPLARQVLQGKKVVKLEISGK 583

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           QK+TGVKSRRKSVASSGL YKLDELRKELAS+DG IFPHSVLSTQQIG+LSAQKPTSLDQ
Sbjct: 584 QKITGVKSRRKSVASSGLEYKLDELRKELASIDGDIFPHSVLSTQQIGVLSAQKPTSLDQ 643

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                      LETMIGKVKAEKYGNRIL EIEKWS  Q+Q+AS
Sbjct: 644 ---------------------------LETMIGKVKAEKYGNRILGEIEKWSAAQSQNAS 703

Query: 181 QEEQEDENRTPKRPKTINSHVVIESSDEEA 211
           QEE+ DENR PKRPKTI++HVVIESSDEEA
Sbjct: 704 QEEKGDENRAPKRPKTIDTHVVIESSDEEA 706

[Close]

BLAST of Lsi05G011520 vs. TAIR 10
Match: AT1G31360.1 (RECQ helicase L2 )

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-41
Identity = 96/211 (45.50%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           +++L+++E+E L+I+L++D VLKEEFQHT Y+TNAYVT+GPLA Q+LQG+K +K+E S +
Sbjct: 524 IAELKRDEVEHLVIKLIVDSVLKEEFQHTPYSTNAYVTMGPLANQLLQGRKTIKMETSSR 583

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           Q     K  ++S+  SGL  KLDELRKE+++ DG I PH+VLSTQQIG +S+QKP SL  
Sbjct: 584 Q----TKKLKRSITFSGLELKLDELRKEISAADGSILPHTVLSTQQIGSISSQKPVSLQ- 643

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                     +LE++IGK+K EKYG+RILEE+ +      Q   
Sbjct: 644 --------------------------ELESIIGKLKTEKYGDRILEEVMRHEAVSEQLVE 703

Query: 181 QEEQED--ENRTPKRPKTINSHVVIESSDEE 210
              +E+  ++R  KR KT    V++ESS EE
Sbjct: 704 DPTKEETCKSRLRKRAKTQKDVVLVESSGEE 703

[Close]

BLAST of Lsi05G011520 vs. TAIR 10
Match: AT1G31360.2 (RECQ helicase L2 )

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-41
Identity = 96/211 (45.50%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   VSDLRKEEIEQLIIQLLLDRVLKEEFQHTAYATNAYVTVGPLARQVLQGKKVVKLEISGK 60
           +++L+++E+E L+I+L++D VLKEEFQHT Y+TNAYVT+GPLA Q+LQG+K +K+E S +
Sbjct: 399 IAELKRDEVEHLVIKLIVDSVLKEEFQHTPYSTNAYVTMGPLANQLLQGRKTIKMETSSR 458

Query: 61  QKVTGVKSRRKSVASSGLGYKLDELRKELASVDGGIFPHSVLSTQQIGLLSAQKPTSLDQ 120
           Q     K  ++S+  SGL  KLDELRKE+++ DG I PH+VLSTQQIG +S+QKP SL  
Sbjct: 459 Q----TKKLKRSITFSGLELKLDELRKEISAADGSILPHTVLSTQQIGSISSQKPVSLQ- 518

Query: 121 ASFLLSILSTEFENGGCWLIRKPLYTDLETMIGKVKAEKYGNRILEEIEKWSIGQNQSAS 180
                                     +LE++IGK+K EKYG+RILEE+ +      Q   
Sbjct: 519 --------------------------ELESIIGKLKTEKYGDRILEEVMRHEAVSEQLVE 578

Query: 181 QEEQED--ENRTPKRPKTINSHVVIESSDEE 210
              +E+  ++R  KR KT    V++ESS EE
Sbjct: 579 DPTKEETCKSRLRKRAKTQKDVVLVESSGEE 578

The following BLAST results are available for this feature:

BLAST of Lsi05G011520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FT73	3.5e-40	45.50	ATP-dependent DNA helicase Q-like 2 OS=Arabidopsis thaliana OX=3702 GN=RECQL2 PE...	[more]

BLAST of Lsi05G011520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HTR5	1.4e-76	78.10	ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111466807 PE=3 SV=1	[more]
A0A6J1HRC8	1.4e-76	78.10	DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111466807 PE=3 SV=1	[more]
A0A6J1HQ66	1.4e-76	78.10	ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111466807 PE=3 SV=1	[more]
A0A0A0L974	1.4e-76	79.62	ATP-dependent DNA helicase OS=Cucumis sativus OX=3659 GN=Csa_3G636420 PE=3 SV=1	[more]
A0A6J1HGH1	1.4e-76	78.57	DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111464077 PE=3 SV=1	[more]

BLAST of Lsi05G011520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887820.1	5.7e-80	81.90	LOW QUALITY PROTEIN: ATP-dependent DNA helicase Q-like 2 [Benincasa hispida]	[more]
KAG6571606.1	2.4e-78	80.00	ATP-dependent DNA helicase Q-like 2, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023511413.1	4.5e-77	79.05	mediator of RNA polymerase II transcription subunit 34 isoform X2 [Cucurbita pep...	[more]
XP_023511414.1	4.5e-77	79.05	mediator of RNA polymerase II transcription subunit 34 isoform X3 [Cucurbita pep...	[more]
XP_023511412.1	4.5e-77	79.05	mediator of RNA polymerase II transcription subunit 34 isoform X1 [Cucurbita pep...	[more]

BLAST of Lsi05G011520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G31360.1	2.5e-41	45.50	RECQ helicase L2	[more]
AT1G31360.2	2.5e-41	45.50	RECQ helicase L2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..59 e-value: 2.5E-16 score: 61.5
IPR018982	RQC domain	PFAM	PF09382	RQC	coord: 2..61 e-value: 1.7E-5 score: 24.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G011520.1	Lsi05G011520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006268	DNA unwinding involved in DNA replication
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0006281	DNA repair
biological_process	GO:0006260	DNA replication
cellular_component	GO:0005694	chromosome
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0043138	3'-5' DNA helicase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0009378	four-way junction helicase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003676	nucleic acid binding