Lsi05G011290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G011290
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Poly(A)-specific ribonuclease
Location	chr05: 19071176 .. 19072064 (-)
RNA-Seq Expression	Lsi05G011290
Synteny	Lsi05G011290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATCAGATAGGGAAATCGCCTGAAAGAAATCAGAGAAGAAAGAATAAGACAAGTGGAGAATCGCGGATCAGTCCATCCGATTGGAAACGAGAGGTTTGGTCTGAGAATCTCCGGGAAGAAACAGAGCTTCTGAAACAATATCTTCCAAAATTTCCGATAGTCGCCATTGATACAGAATTTCCAGGTATATTTATATGTGTAAATTGATGGATACATCGTTTAGATTTTAGAGTTTGGTTTTACCGTTAGGAATGGATAGATTGTTTCTTCTAGGATTTCTTCGATACACTCCAAAAGGAGGAAGAATTTTACCATTGCCTTCAATTCAATGTGAATCGATTGAGAATACTTCAATTGGGTCTCACAGTATGTGACGAGAAGGGAAATATAGGAATGACTTGGGAGTTTAATTTCAGAGATTTCGATCAGATTTCCGATATGGAAGGGGTTTCTTCGTTGGAGTTCTTTTTGAAGAACAAAGGTTTTGATTTTGAGAAATTGAGAAGCAATGGAATTGTGCCAGAGGAATTTGGTAAACTGTTCGAACCCATTCTTCGAATTGGTGGAATCCAAATCTGGATTACCTTTCATGGATTTTACGACATTGCTTATCTTATGAAGCTTCTCGATATTAGATCTATGCTGACTTCCATGGCTGCCTTTGCTATCACTGTACTGCAACGGCTTGCTCGACGGCAACTTAGGTCTAAAAAAACTGGCTAAATTGCTCCATGTTAACCGAATCGGTATTGCACATTTTGCTGGTTCTGATAGTTTGCTCACGGCCTCTGTTTATGCCAATATGAAGACCCTGTTCAAGCTCCAGGAAAGCCTCTCTGAAGGCTTTTTGTACGGATTGCCTCACCCGATTCGAGCTCTCTGA

mRNA sequence

ATGAAGAATCAGATAGGGAAATCGCCTGAAAGAAATCAGAGAAGAAAGAATAAGACAAGTGGAGAATCGCGGATCAGTCCATCCGATTGGAAACGAGAGGTTTGGTCTGAGAATCTCCGGGAAGAAACAGAGCTTCTGAAACAATATCTTCCAAAATTTCCGATAGTCGCCATTGATACAGAATTTCCAGGTATATTTATATGTGATTTCTTCGATACACTCCAAAAGGAGGAAGAATTTTACCATTGCCTTCAATTCAATGTGAATCGATTGAGAATACTTCAATTGGGTCTCACAGTATGTGACGAGAAGGGAAATATAGGAATGACTTGGGAGTTTAATTTCAGAGATTTCGATCAGATTTCCGATATGGAAGGGGTTTCTTCGTTGGAGTTCTTTTTGAAGAACAAAGGTTTTGATTTTGAGAAATTGAGAAGCAATGGAATTGTGCCAGAGGAATTTGGTAAACTGTTCGAACCCATTCTTCGAATTGGTGGAATCCAAATCTGGATTACCTTTCATGGATTTTACGACATTGCTTATCTTATGAAGCTTCTCGATATTAGATCTATGCTGACTTCCATGGCTGCCTTTGCTATCACTGTACTGCAACGGCTTGCTCGACGGCAACTTAGTTTGCTCACGGCCTCTGTTTATGCCAATATGAAGACCCTGTTCAAGCTCCAGGAAAGCCTCTCTGAAGGCTTTTTGTACGGATTGCCTCACCCGATTCGAGCTCTCTGA

Coding sequence (CDS)

ATGAAGAATCAGATAGGGAAATCGCCTGAAAGAAATCAGAGAAGAAAGAATAAGACAAGTGGAGAATCGCGGATCAGTCCATCCGATTGGAAACGAGAGGTTTGGTCTGAGAATCTCCGGGAAGAAACAGAGCTTCTGAAACAATATCTTCCAAAATTTCCGATAGTCGCCATTGATACAGAATTTCCAGGTATATTTATATGTGATTTCTTCGATACACTCCAAAAGGAGGAAGAATTTTACCATTGCCTTCAATTCAATGTGAATCGATTGAGAATACTTCAATTGGGTCTCACAGTATGTGACGAGAAGGGAAATATAGGAATGACTTGGGAGTTTAATTTCAGAGATTTCGATCAGATTTCCGATATGGAAGGGGTTTCTTCGTTGGAGTTCTTTTTGAAGAACAAAGGTTTTGATTTTGAGAAATTGAGAAGCAATGGAATTGTGCCAGAGGAATTTGGTAAACTGTTCGAACCCATTCTTCGAATTGGTGGAATCCAAATCTGGATTACCTTTCATGGATTTTACGACATTGCTTATCTTATGAAGCTTCTCGATATTAGATCTATGCTGACTTCCATGGCTGCCTTTGCTATCACTGTACTGCAACGGCTTGCTCGACGGCAACTTAGTTTGCTCACGGCCTCTGTTTATGCCAATATGAAGACCCTGTTCAAGCTCCAGGAAAGCCTCTCTGAAGGCTTTTTGTACGGATTGCCTCACCCGATTCGAGCTCTCTGA

Protein sequence

MKNQIGKSPERNQRRKNKTSGESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAAFAITVLQRLARRQLSLLTASVYANMKTLFKLQESLSEGFLYGLPHPIRAL

Homology

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BLAST of Lsi05G011290 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 4.0e-27
Identity = 82/218 (37.61%), Postives = 115/218 (52.75%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEF-YHCLQFNVNR 91
           REVW++NL  E  L+++ +  FP VA+DTEFPGI +C    T +   E+ Y  L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPGI-VCRPVGTFKTNTEYHYETLKTNVNI 72

Query: 92  LRILQLGLTVCDEKGNIG--------MTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFE 151
           L+++QLGLT  DEKGN+           W+FNFR+FD  SD+    S+E  L+  G DF 
Sbjct: 73  LKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDFV 132

Query: 152 KLRSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSMLTSMAA 211
           K    GI  + F +L    L   GI +     W+TFH  YD  YL+KLL  +++  +   
Sbjct: 133 KNNEFGIDSKRFAEL----LMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTG 192

Query: 212 FAITVLQRLARRQLSLLTASVYANMKTLFKLQESLSEG 236
           F            +S+    VY ++K L K   SL  G
Sbjct: 193 FF---------EMISVYFPRVY-DIKHLMKFCNSLHGG 214

[Close]

BLAST of Lsi05G011290 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.0e-26
Identity = 68/178 (38.20%), Postives = 100/178 (56.18%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPG-IFICDFFDTLQKEEEFYHCLQFNVNR 91
           REVW+ENL  E EL+ + +  +P +++DTEFPG IF  D   T    ++ Y  L+ NV+ 
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFT--NPDDLYTLLKANVDA 79

Query: 92  LRILQLGLTVCDEKGNI---------GMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDF 151
           L ++Q+GLT+ D  GN+         G  WEFNFRDFD   D     S+E  L+ +G DF
Sbjct: 80  LSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGIDF 139

Query: 152 EKLRSNGIVPEEFGKLFEPILRIGGIQI-WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
           E+   +G+  E F +L      +   ++ W+TFH  YD  YLMK+L  R +  ++  F
Sbjct: 140 ERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRRELPGALGEF 194

[Close]

BLAST of Lsi05G011290 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.6e-26
Identity = 70/179 (39.11%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVNRL 91
           REVW++NL+EE +L++  +  FP VA+DTEFPGI +          +  Y  L+ NVN L
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADYHYETLKTNVNIL 72

Query: 92  RILQLGLTVCDEKGNIG-------MTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKL 151
           +++QLGLT  +E+GN+          W+FNFR+FD  SD+  + S+E  LK  G D  K 
Sbjct: 73  KMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKN 132

Query: 152 RSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
             +GI  + F +L    L   GI +     W+TFH  YD  YL+KLL  +++  S   F
Sbjct: 133 TLDGIDSKRFAEL----LMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 186

[Close]

BLAST of Lsi05G011290 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.3e-25
Identity = 70/186 (37.63%), Postives = 101/186 (54.30%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEF-------YHCL 91
           REVW+ NL +E  L++Q +  FP VA+DTEFPGI +C           +       Y  L
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGI-VCKTVTANPNPNPYSIHYEYNYDTL 76

Query: 92  QFNVNRLRILQLGLTVCDEKGNI-------GMTWEFNFRDFDQISDMEGVSSLEFFLKNK 151
           + NVN L+++QLGLT+ DEKGN+          W+FNFR+F+ ISDM  + S+E  L+  
Sbjct: 77  KANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKS 136

Query: 152 GFDFEKLRSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSML 199
             D EK    G+  + F +L    L   G+ +     W+TFH  YD  YL+KLL  + + 
Sbjct: 137 AIDLEKNNECGVDAKRFAEL----LMGSGVVLNDKIHWVTFHCGYDFGYLLKLLSGKELP 196

[Close]

BLAST of Lsi05G011290 vs. ExPASy Swiss-Prot
Match: Q9FMS6 (Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=CAF1-11 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 7.0e-24
Identity = 63/179 (35.20%), Postives = 96/179 (53.63%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQK--EEEFYHCLQFNVN 91
           R+VW+ NL  E +L++  +  +P +++DTEFPG+      D L++      Y+ L+ NV+
Sbjct: 15  RDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGNPNYLYNLLKSNVD 74

Query: 92  RLRILQLGLTVCDEKGNI---------GMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFD 151
            L ++Q+GLT+ D  GN+            WEFNFRDFD   D     S+E  L+  G D
Sbjct: 75  ALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIE-LLRRHGID 134

Query: 152 FEKLRSNGIVPEEFGKLFEPILRIGGIQI-WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
           FE+ R  G+  E F +L      I    + W+TFH  YD  YL+K+L  R +  ++  F
Sbjct: 135 FERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPVALREF 192

[Close]

BLAST of Lsi05G011290 vs. ExPASy TrEMBL
Match: A0A6J1HL76 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 4.5e-66
Identity = 147/265 (55.47%), Postives = 171/265 (64.53%), Query Frame = 0


Query: 18  KTSGESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKE 77
           K  GE  I    WKR+VWS NL EE EL+KQ L +FPIVAIDTEFPG     +      +
Sbjct: 3   KMIGEVAIVEGQWKRDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPGFL--RWTPKGVSD 62

Query: 78  EEFYHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNK 137
           EEFY CLQFNVNRL ILQLGLT+ ++ G IGMTWEFNFRDFD+IS+MEGV SL+ FLKNK
Sbjct: 63  EEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTWEFNFRDFDKISNMEGVYSLDSFLKNK 122

Query: 138 GFDFEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAA 197
           GF+F  LRS GI PEEFG  F PILR G I  WITFHG YDIAYLMK  +IR M TSM+ 
Sbjct: 123 GFNFNTLRSYGIAPEEFGTQFSPILRSGRIHRWITFHGLYDIAYLMKFTNIRCMPTSMSI 182

Query: 198 FAITV------------------------LQRLAR-------------RQLSLLTASVYA 246
           FAIT                         L++LA+                SLLTASVYA
Sbjct: 183 FAITAAHILGTVTDLKHMAWYSLLDGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYA 242

[Close]

BLAST of Lsi05G011290 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.3e-65
Identity = 143/255 (56.08%), Postives = 171/255 (67.06%), Query Frame = 0


Query: 30  WKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVN 89
           WKREVWS NL EE EL+K YL +FPIVAIDTEFPG     +      +EEFY CLQFNVN
Sbjct: 15  WKREVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFL--RWTPKGVSDEEFYQCLQFNVN 74

Query: 90  RLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKLRSNGI 149
           RL ILQLGLT+ D+ G IGMTWEFNF+DFDQIS+MEGV SL+ FLKNKGF+F KLRS GI
Sbjct: 75  RLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGI 134

Query: 150 VPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAAFA---------I 209
            P+EF + F+P++R G IQ WITFHG YDIAYLMK  +I SM TSM+ FA         +
Sbjct: 135 APQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTV 194

Query: 210 TVLQRLAR------------RQL------------------SLLTASVYANMKTLFKLQE 246
           T L+ +AR            ++L                  SLLTASVYA MK + K++ 
Sbjct: 195 TDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVIMKVEP 254

[Close]

BLAST of Lsi05G011290 vs. ExPASy TrEMBL
Match: A0A6J1HRD6 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467091 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.5e-64
Identity = 146/267 (54.68%), Postives = 171/267 (64.04%), Query Frame = 0


Query: 18  KTSGESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKE 77
           K  GE+ I    WKR+VWS NL EE EL+K  L +FPIVA+DTEFPG   C        +
Sbjct: 3   KIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLTCT--PKGVSD 62

Query: 78  EEFYHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNK 137
           EEFY CLQFNVNRL ILQLGLT+ DE G IGMTWEFNF+DFD+IS+MEGV SL+ FLKNK
Sbjct: 63  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 122

Query: 138 GFDFEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAA 197
           GF+F  LRS GI PEEF + F PILR G IQ WITFHG YDIAYLMK  +I  M TSM+ 
Sbjct: 123 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 182

Query: 198 FAI---------TVLQRLAR------------RQL------------------SLLTASV 246
           FAI         T L+ +AR            ++L                  SLLTASV
Sbjct: 183 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 242

[Close]

BLAST of Lsi05G011290 vs. ExPASy TrEMBL
Match: A0A6J1HSR3 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467090 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 4.2e-64
Identity = 146/267 (54.68%), Postives = 171/267 (64.04%), Query Frame = 0


Query: 18  KTSGESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKE 77
           K  GE+ I    WKR+VWS NL EE EL+K  L +FPIVA+DTEFPG   C        +
Sbjct: 3   KIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLRCT--PKGVSD 62

Query: 78  EEFYHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNK 137
           EEFY CLQFNVNRL ILQLGLT+ DE G IGMTWEFNF+DFD+IS+MEGV SL+ FLKNK
Sbjct: 63  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 122

Query: 138 GFDFEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAA 197
           GF+F  LRS GI PEEF + F PILR G IQ WITFHG YDIAYLMK  +I  M TSM+ 
Sbjct: 123 GFNFNILRSCGISPEEFRRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 182

Query: 198 FAI---------TVLQRLAR------------RQL------------------SLLTASV 246
           FAI         T L+ +AR            ++L                  SLLTASV
Sbjct: 183 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 242

[Close]

BLAST of Lsi05G011290 vs. ExPASy TrEMBL
Match: A0A6J1HIZ7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463978 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 4.2e-64
Identity = 142/264 (53.79%), Postives = 172/264 (65.15%), Query Frame = 0


Query: 21  GESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEF 80
           GE       W+R+VWS NL EE EL++Q L +FPIVA+DTEFPG     +      +EEF
Sbjct: 3   GEVATVEGQWERDVWSHNLLEEIELIQQCLSEFPIVAMDTEFPGFL--RWTPKGVSDEEF 62

Query: 81  YHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFD 140
           Y CLQFNVNRL ILQLGLT+ ++ G IGMTWEFNF+DFD+IS+MEGV SL+ FLKNKGF+
Sbjct: 63  YECLQFNVNRLSILQLGLTLTNDNGKIGMTWEFNFKDFDKISNMEGVYSLDSFLKNKGFN 122

Query: 141 FEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAAFAI 200
           F  LRS GI PEEFG+ F PILR G I  WITFHG YDIAYLMKL +I  M TSM+ FAI
Sbjct: 123 FNTLRSYGIAPEEFGRQFNPILRSGRIHRWITFHGLYDIAYLMKLTNITCMPTSMSIFAI 182

Query: 201 TV---------LQRLAR------------RQL------------------SLLTASVYAN 246
           T          L+ +AR            ++L                  SLLTASVYA 
Sbjct: 183 TAAHLLGTVTDLKHMARCSNYLFNGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAK 242

[Close]

BLAST of Lsi05G011290 vs. NCBI nr
Match: XP_022963784.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 261.2 bits (666), Expect = 9.3e-66
Identity = 147/265 (55.47%), Postives = 171/265 (64.53%), Query Frame = 0


Query: 18  KTSGESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKE 77
           K  GE  I    WKR+VWS NL EE EL+KQ L +FPIVAIDTEFPG     +      +
Sbjct: 3   KMIGEVAIVEGQWKRDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPGFL--RWTPKGVSD 62

Query: 78  EEFYHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNK 137
           EEFY CLQFNVNRL ILQLGLT+ ++ G IGMTWEFNFRDFD+IS+MEGV SL+ FLKNK
Sbjct: 63  EEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTWEFNFRDFDKISNMEGVYSLDSFLKNK 122

Query: 138 GFDFEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAA 197
           GF+F  LRS GI PEEFG  F PILR G I  WITFHG YDIAYLMK  +IR M TSM+ 
Sbjct: 123 GFNFNTLRSYGIAPEEFGTQFSPILRSGRIHRWITFHGLYDIAYLMKFTNIRCMPTSMSI 182

Query: 198 FAITV------------------------LQRLAR-------------RQLSLLTASVYA 246
           FAIT                         L++LA+                SLLTASVYA
Sbjct: 183 FAITAAHILGTVTDLKHMAWYSLLDGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYA 242

[Close]

BLAST of Lsi05G011290 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 259.6 bits (662), Expect = 2.7e-65
Identity = 143/255 (56.08%), Postives = 171/255 (67.06%), Query Frame = 0


Query: 30  WKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVN 89
           WKREVWS NL EE EL+K YL +FPIVAIDTEFPG     +      +EEFY CLQFNVN
Sbjct: 15  WKREVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFL--RWTPKGVSDEEFYQCLQFNVN 74

Query: 90  RLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKLRSNGI 149
           RL ILQLGLT+ D+ G IGMTWEFNF+DFDQIS+MEGV SL+ FLKNKGF+F KLRS GI
Sbjct: 75  RLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGI 134

Query: 150 VPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAAFA---------I 209
            P+EF + F+P++R G IQ WITFHG YDIAYLMK  +I SM TSM+ FA         +
Sbjct: 135 APQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTV 194

Query: 210 TVLQRLAR------------RQL------------------SLLTASVYANMKTLFKLQE 246
           T L+ +AR            ++L                  SLLTASVYA MK + K++ 
Sbjct: 195 TDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVIMKVEP 254

[Close]

BLAST of Lsi05G011290 vs. NCBI nr
Match: XP_022967647.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 256.1 bits (653), Expect = 3.0e-64
Identity = 146/267 (54.68%), Postives = 171/267 (64.04%), Query Frame = 0


Query: 18  KTSGESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKE 77
           K  GE+ I    WKR+VWS NL EE EL+K  L +FPIVA+DTEFPG   C        +
Sbjct: 3   KIIGEAAIVGGKWKRDVWSHNLLEEIELMKHCLSEFPIVAMDTEFPGFLTCT--PKGVSD 62

Query: 78  EEFYHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNK 137
           EEFY CLQFNVNRL ILQLGLT+ DE G IGMTWEFNF+DFD+IS+MEGV SL+ FLKNK
Sbjct: 63  EEFYQCLQFNVNRLSILQLGLTLTDENGRIGMTWEFNFQDFDKISNMEGVYSLDSFLKNK 122

Query: 138 GFDFEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAA 197
           GF+F  LRS GI PEEF + F PILR G IQ WITFHG YDIAYLMK  +I  M TSM+ 
Sbjct: 123 GFNFNILRSCGISPEEFSRQFNPILRSGRIQRWITFHGLYDIAYLMKFTNIICMPTSMSN 182

Query: 198 FAI---------TVLQRLAR------------RQL------------------SLLTASV 246
           FAI         T L+ +AR            ++L                  SLLTASV
Sbjct: 183 FAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKMLQVNRIGTAHFAGSDSLLTASV 242

[Close]

BLAST of Lsi05G011290 vs. NCBI nr
Match: XP_023550519.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.8 bits (652), Expect = 3.9e-64
Identity = 143/255 (56.08%), Postives = 169/255 (66.27%), Query Frame = 0


Query: 30  WKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVN 89
           WKREVWS NL EE EL+K YL +FPIVA+DTEFPG     +      +EEFY CLQFNVN
Sbjct: 13  WKREVWSYNLLEEIELMKHYLSEFPIVAMDTEFPGFL--RWTPKGVSDEEFYQCLQFNVN 72

Query: 90  RLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKLRSNGI 149
           RL ILQLGLT+ DE G IGMTWEFNF+DFD+IS+MEGV SL+ FLKNKGF+F KLRS GI
Sbjct: 73  RLSILQLGLTLIDENGKIGMTWEFNFKDFDKISNMEGVYSLDSFLKNKGFNFNKLRSCGI 132

Query: 150 VPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAAFAI--------- 209
            PEEF + F P++R   IQ WITFHG YDIAYLMK  +I SM TSM+ FAI         
Sbjct: 133 APEEFSRQFNPVIRSRRIQRWITFHGLYDIAYLMKFNNIISMPTSMSMFAIIAAHLLGTV 192

Query: 210 TVLQRLAR------------RQL------------------SLLTASVYANMKTLFKLQE 246
           T L+ +AR            ++L                  SLLTASVYA MK + K++ 
Sbjct: 193 TDLKHMARYSDYLFNGNLGLKKLAKLLQINRIGTAHFAGSDSLLTASVYAKMKAIMKVEP 252

[Close]

BLAST of Lsi05G011290 vs. NCBI nr
Match: XP_022963785.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 254.6 bits (649), Expect = 8.7e-64
Identity = 142/264 (53.79%), Postives = 172/264 (65.15%), Query Frame = 0


Query: 21  GESRISPSDWKREVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEF 80
           GE       W+R+VWS NL EE EL++Q L +FPIVA+DTEFPG     +      +EEF
Sbjct: 3   GEVATVEGQWERDVWSHNLLEEIELIQQCLSEFPIVAMDTEFPGFL--RWTPKGVSDEEF 62

Query: 81  YHCLQFNVNRLRILQLGLTVCDEKGNIGMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFD 140
           Y CLQFNVNRL ILQLGLT+ ++ G IGMTWEFNF+DFD+IS+MEGV SL+ FLKNKGF+
Sbjct: 63  YECLQFNVNRLSILQLGLTLTNDNGKIGMTWEFNFKDFDKISNMEGVYSLDSFLKNKGFN 122

Query: 141 FEKLRSNGIVPEEFGKLFEPILRIGGIQIWITFHGFYDIAYLMKLLDIRSMLTSMAAFAI 200
           F  LRS GI PEEFG+ F PILR G I  WITFHG YDIAYLMKL +I  M TSM+ FAI
Sbjct: 123 FNTLRSYGIAPEEFGRQFNPILRSGRIHRWITFHGLYDIAYLMKLTNITCMPTSMSIFAI 182

Query: 201 TV---------LQRLAR------------RQL------------------SLLTASVYAN 246
           T          L+ +AR            ++L                  SLLTASVYA 
Sbjct: 183 TAAHLLGTVTDLKHMARCSNYLFNGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAK 242

[Close]

BLAST of Lsi05G011290 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.8e-28
Identity = 82/218 (37.61%), Postives = 115/218 (52.75%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEF-YHCLQFNVNR 91
           REVW++NL  E  L+++ +  FP VA+DTEFPGI +C    T +   E+ Y  L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPGI-VCRPVGTFKTNTEYHYETLKTNVNI 72

Query: 92  LRILQLGLTVCDEKGNIG--------MTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFE 151
           L+++QLGLT  DEKGN+           W+FNFR+FD  SD+    S+E  L+  G DF 
Sbjct: 73  LKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDFV 132

Query: 152 KLRSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSMLTSMAA 211
           K    GI  + F +L    L   GI +     W+TFH  YD  YL+KLL  +++  +   
Sbjct: 133 KNNEFGIDSKRFAEL----LMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTG 192

Query: 212 FAITVLQRLARRQLSLLTASVYANMKTLFKLQESLSEG 236
           F            +S+    VY ++K L K   SL  G
Sbjct: 193 FF---------EMISVYFPRVY-DIKHLMKFCNSLHGG 214

[Close]

BLAST of Lsi05G011290 vs. TAIR 10
Match: AT3G44260.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.4e-27
Identity = 68/178 (38.20%), Postives = 100/178 (56.18%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPG-IFICDFFDTLQKEEEFYHCLQFNVNR 91
           REVW+ENL  E EL+ + +  +P +++DTEFPG IF  D   T    ++ Y  L+ NV+ 
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFT--NPDDLYTLLKANVDA 79

Query: 92  LRILQLGLTVCDEKGNI---------GMTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDF 151
           L ++Q+GLT+ D  GN+         G  WEFNFRDFD   D     S+E  L+ +G DF
Sbjct: 80  LSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGIDF 139

Query: 152 EKLRSNGIVPEEFGKLFEPILRIGGIQI-WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
           E+   +G+  E F +L      +   ++ W+TFH  YD  YLMK+L  R +  ++  F
Sbjct: 140 ERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRRELPGALGEF 194

[Close]

BLAST of Lsi05G011290 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-27
Identity = 70/179 (39.11%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVNRL 91
           REVW++NL+EE +L++  +  FP VA+DTEFPGI +          +  Y  L+ NVN L
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADYHYETLKTNVNIL 72

Query: 92  RILQLGLTVCDEKGNIG-------MTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKL 151
           +++QLGLT  +E+GN+          W+FNFR+FD  SD+  + S+E  LK  G D  K 
Sbjct: 73  KMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKN 132

Query: 152 RSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
             +GI  + F +L    L   GI +     W+TFH  YD  YL+KLL  +++  S   F
Sbjct: 133 TLDGIDSKRFAEL----LMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 186

[Close]

BLAST of Lsi05G011290 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-27
Identity = 70/179 (39.11%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVNRL 91
           REVW++NL+EE +L++  +  FP VA+DTEFPGI +          +  Y  L+ NVN L
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADYHYETLKTNVNIL 72

Query: 92  RILQLGLTVCDEKGNIG-------MTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKL 151
           +++QLGLT  +E+GN+          W+FNFR+FD  SD+  + S+E  LK  G D  K 
Sbjct: 73  KMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKN 132

Query: 152 RSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
             +GI  + F +L    L   GI +     W+TFH  YD  YL+KLL  +++  S   F
Sbjct: 133 TLDGIDSKRFAEL----LMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 186

[Close]

BLAST of Lsi05G011290 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-27
Identity = 70/179 (39.11%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 32  REVWSENLREETELLKQYLPKFPIVAIDTEFPGIFICDFFDTLQKEEEFYHCLQFNVNRL 91
           REVW++NL+EE +L++  +  FP VA+DTEFPGI +          +  Y  L+ NVN L
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADYHYETLKTNVNIL 72

Query: 92  RILQLGLTVCDEKGNIG-------MTWEFNFRDFDQISDMEGVSSLEFFLKNKGFDFEKL 151
           +++QLGLT  +E+GN+          W+FNFR+FD  SD+  + S+E  LK  G D  K 
Sbjct: 73  KMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKN 132

Query: 152 RSNGIVPEEFGKLFEPILRIGGIQI-----WITFHGFYDIAYLMKLLDIRSMLTSMAAF 199
             +GI  + F +L    L   GI +     W+TFH  YD  YL+KLL  +++  S   F
Sbjct: 133 TLDGIDSKRFAEL----LMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 186

The following BLAST results are available for this feature:

BLAST of Lsi05G011290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SKZ2	4.0e-27	37.61	Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LXM2	2.0e-26	38.20	Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SAI2	2.6e-26	39.11	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9S9P2	8.3e-25	37.63	Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9FMS6	7.0e-24	35.20	Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi05G011290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HL76	4.5e-66	55.47	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3...	[more]
A0A6J1ESM7	1.3e-65	56.08	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3...	[more]
A0A6J1HRD6	1.5e-64	54.68	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467091 PE=3 S...	[more]
A0A6J1HSR3	4.2e-64	54.68	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111467090 PE=3 S...	[more]
A0A6J1HIZ7	4.2e-64	53.79	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463978 PE=3...	[more]

BLAST of Lsi05G011290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022963784.1	9.3e-66	55.47	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_022928830.1	2.7e-65	56.08	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_022967647.1	3.0e-64	54.68	putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima]	[more]
XP_023550519.1	3.9e-64	56.08	putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo]	[more]
XP_022963785.1	8.7e-64	53.79	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]

BLAST of Lsi05G011290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32070.1	2.8e-28	37.61	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G44260.1	1.4e-27	38.20	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.1	1.8e-27	39.11	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	1.8e-27	39.11	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.3	1.8e-27	39.11	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 26..206 e-value: 2.0E-44 score: 153.6
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 34..149 e-value: 2.8E-9 score: 36.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..26
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 31..205
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 31..205
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 31..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G011290.1	Lsi05G011290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0005622	intracellular anatomical structure
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity