Lsi05G010150 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G010150
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionactin-depolymerizing factor-like
Locationchr05: 17617219 .. 17620599 (-)
RNA-Seq ExpressionLsi05G010150
SyntenyLsi05G010150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAATGAACACTAAAATTTGGGTCGAAAGGGAAAGGGAAAGAGAAGAATATTTCAGTTTGATTGGGAAATTGGCAGGATTGGCTAAGCCACTGGGTCTAAGTTCCTTTCCCTTTCTCTTCTTTCTATATCTTCTGCCTTGGCCTCCGATTCTTGCCTACCACTTCTGACCTTTCTCTTATTCCATTCTTCTTCTTCTTCTTCTTTTTCTTCTTCTTCTTCTTCCTTTCCTCAAGTTTTCTCATCGTCTTTCTCATATCCACCTCTTCTCCGATCGTCATGTCGTTCAGAGGCCTCCGACGGGTATCTTTATGTTCTTCCTTTTATGTTTTCTTTACTTTAACTCAACTGGGTCTCACCATATTTCAATTGGGGGTTCTGGGTTTTCTTTCGTTTTAATGCTAATTATGTTTATCTCAAGATTCATAACCCCTGCTTCATCGTTCAGACCCACCCATCAACTGAATCCTGAATTTTTGTGTATTTTATGACTTTAATTTCTTTCTTTCCCCCAGTTCTGTGATGGGTTTACTTTTGTTTCTGCAAGAATGGTGTCTTTTGTTTTTCTTTCCTTTATTTCTTGGAGGCATATTTCAAAAGACTAAGAGAGTACTGTGGATTTGGATGAAAAAAGTTGGATTAGAAAGATCATGTGCTTGATTTAAGGTTCATATTATAGCTCAGAAAGCTTTGATTTAAAGGGAAGAAGTCATGATCTTTACTGATCAGGTTTTGGAGGTGAAGGATGGAGTCTGGTTGGTATATGAGCTCTGATTTTGTGCCAGAGTCTGTTGATGGACTTCTGTCTTCTTTGCTTGTTCTAAAATTTCTTTTTAGGAACTTTTCAATTCAGGATTATAAAATTAGAATCTATTGGGGATCTCAGGATTAATCATGGTGTTCTGCTACTCCTTAGTCAAATCCAACTTTTTGCAACAAAAAGAGAACCCATTAATTGAATAATATTATAGATCTCATGGTTCAATTTAAAGGGCTTCTTCTGCTGCTCTATTTTGTTGAGGATTTTTACTTTGTTGTCATCCTTTCCTTTGAAATCTACTGATTTGGAACTCTCTACATGCTTTGGCTCTTACTGTTACTTGTTGATGCTTTATGGACCTTTGTTTGAGTGTTTGACATTTATGAATTTACCTTTGTAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACATTTTCTGAACTACAAAGAAAGAAAATGTATCGATATGTGATCTTTAAGGTCGATGAAAAGAAAAGAGAGGTGGTGGTTGACAAGATTGGCAATCCTGCAGAGAGCTATGAAGATTTCACTGCTGCTCTGCCCGACAATGATTGCCGCTACGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGCCAGAAGAGCAAGATATTTTTTATAGCATGGTTAGCTTTCACCATCATGAACTTAGTCTTATCTTATTTCTTATTGCTATGTTAATGTCTACTTGATATATATTGTTCATTGAGTTTCCCCCACAATGCAAAATTTTGGTACATACTAAGGAAAACATTACTTCTCCTAAGGTCATAAGGTTTTGATCCTATGATCACCTATGACTATTTAAATTCAAACTACCATTTCTAAGCTCTAGCCTCTAGGGTTCTTCATGTTCCTGGTCAGTTGCTCATTCATGGTATACTTGCTAATTTCATTACATACCTTCTGGTGCATATGCTTTACTCTCCACACTGCAGAAGTTGGAGTTTAGTGGTTAGAGCTCTGTATGCATCCTCTAATCTCTGTGAAGATCATAGTACATCAAAAGTTTTGGGCAAAACTTGAAGATCTTGTCATGTTTTGTCAGAATGATTGACGTTTCTGTTGTTGATGATCTAATGCACTTGTTGAATATCTCATTCCAAGAACCATCTATGTTATGACCTTGATTAGAAAGAATAGTTTTAATCATGTCATATCAACGACTATCGTTGGGGTGTCTTTTCTCTCTCCGAACTGCCGAATCGGAAAAATGGAGTGATCTCACACTAGATTGTTGTACTTCAGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTCGATGGCATCCACTATGAAATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATCAGAGACCGTGCTCAATGAACATTCTCTGTTTGTTTAATTTGTTGTTCCCTTGGGGGTAAGCCAGCTTGTATTTTGAATACTTATGAATTGGTTTATCCCTGCTTCGATATGTGTAAAACCGATCGTTGGAAAATGAAAACATTGCACAAAACCGTAAAATAAACTAACATCTGCAGAATGCTGATATATGGTGGATCGACATGATGTTGCTGTCTGGTTGCTATGACTGCTTCTCAGATGAAGATAAGTTTGTAATAGAAAAAGGCTTAGAATCTATCAAACCTTATTATCATTGGAAATTTGTTCTATGATGGATCTGCCTCAATGTCTCTGTGGTTCTTTCATTCTGTCTTTAGAACTTGCATTCGATGATGTGAAATGAAGAACACGAAGCGACGCGCTGTATCGAACATAGCATATTTTGTACTGTCTTTTCATGGACTGAGTTTGAGCTCATCCTCAATGGCATCGATACACAAACAAGTTTCTTCATTAAAATGCATTAACATGTGAATCAAGGACATGTTTCCTTTAAATGCTATTGCTAATTCATGTTGTTAACCAAATATAAAGCAAGAAATAAACAAAAGAAGGCAGATAAAGTACGAACAAGCCGATGGATTTCTAAATTTACTTCACTATCATACTTCTTAGATAGAACAAAAAAATTCTCATAAAGTGACTACATGTCGGCAAAGAAATAGAAATGATAAAAATATATATAGTCACCATATCTAAAGTCACCATATTTTCTAGTCAAATGATCATAAAGCTTTATTCTAATGAGAATTTGATAGATTGAATTTTTAAACATGTGAGACTGTTTCGTTAGTGGTAGATGGTTGGCCTCCTAATTCTCCAATGGTTCCATCTTCATTCTCACCTGACATCTCTTCCAACGATTTTCCTTTTGCTTCAGGCACGAGAAAAGTAAACAAAATTCCCAAAAAGTTAAGTCCACCTAACAATAGAAGCGAGTTCTTGATACCAATGCCAGGAGGATATCCTGCATCAGTTTTGTTCTTGTCTTGGTTTTGAGCCAAATATAGAAAACCAAATGCACCCACCATAGCTCCAAGCTTTCCAGATGCTGATGATACACCGTGGCAAGTAGAACGGAGTCGTGCTGGGAAGATTTCAGCTGGAACAACGAAAGTGGTTGCATTTGGGCCAAAATTTGCAAAGAAAAATGTTAGTGAG

mRNA sequence

CAAAAATGAACACTAAAATTTGGGTCGAAAGGGAAAGGGAAAGAGAAGAATATTTCAGTTTGATTGGGAAATTGGCAGGATTGGCTAAGCCACTGGGTCTAAGTTCCTTTCCCTTTCTCTTCTTTCTATATCTTCTGCCTTGGCCTCCGATTCTTGCCTACCACTTCTGACCTTTCTCTTATTCCATTCTTCTTCTTCTTCTTCTTTTTCTTCTTCTTCTTCTTCCTTTCCTCAAGTTTTCTCATCGTCTTTCTCATATCCACCTCTTCTCCGATCGTCATGTCGTTCAGAGGCCTCCGACGGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACATTTTCTGAACTACAAAGAAAGAAAATGTATCGATATGTGATCTTTAAGGTCGATGAAAAGAAAAGAGAGGTGGTGGTTGACAAGATTGGCAATCCTGCAGAGAGCTATGAAGATTTCACTGCTGCTCTGCCCGACAATGATTGCCGCTACGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGCCAGAAGAGCAAGATATTTTTTATAGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTCGATGGCATCCACTATGAAATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATCAGAGACCGTGCTCAATGAACATTCTCTGTTTGTTTAATTTGTTGTTCCCTTGGGGGTAAGCCAGCTTGTATTTTGAATACTTATGAATTGGTTTATCCCTGCTTCGATATGTGTAAAACCGATCGTTGGAAAATGAAAACATTGCACAAAACCGTAAAATAAACTAACATCTGCAGAATGCTGATATATGGTGGATCGACATGATGTTGCTGTCTGGTTGCTATGACTGCTTCTCAGATGAAGATAAGTTTGTAATAGAAAAAGGCTTAGAATCTATCAAACCTTATTATCATTGGAAATTTGTTCTATGATGGATCTGCCTCAATGTCTCTGTGGTTCTTTCATTCTGTCTTTAGAACTTGCATTCGATGATGTGAAATGAAGAACACGAAGCGACGCGCTGTATCGAACATAGCATATTTTGTACTGTCTTTTCATGGACTGAGTTTGAGCTCATCCTCAATGGCATCGATACACAAACAAGTTTCTTCATTAAAATGCATTAACATGTGAATCAAGGACATGTTTCCTTTAAATGCTATTGCTAATTCATGTTGTTAACCAAATATAAAGCAAGAAATAAACAAAAGAAGGCAGATAAAGTACGAACAAGCCGATGGATTTCTAAATTTACTTCACTATCATACTTCTTAGATAGAACAAAAAAATTCTCATAAAGTGACTACATGTCGGCAAAGAAATAGAAATGATAAAAATATATATAGTCACCATATCTAAAGTCACCATATTTTCTAGTCAAATGATCATAAAGCTTTATTCTAATGAGAATTTGATAGATTGAATTTTTAAACATGTGAGACTGTTTCGTTAGTGGTAGATGGTTGGCCTCCTAATTCTCCAATGGTTCCATCTTCATTCTCACCTGACATCTCTTCCAACGATTTTCCTTTTGCTTCAGGCACGAGAAAAGTAAACAAAATTCCCAAAAAGTTAAGTCCACCTAACAATAGAAGCGAGTTCTTGATACCAATGCCAGGAGGATATCCTGCATCAGTTTTGTTCTTGTCTTGGTTTTGAGCCAAATATAGAAAACCAAATGCACCCACCATAGCTCCAAGCTTTCCAGATGCTGATGATACACCGTGGCAAGTAGAACGGAGTCGTGCTGGGAAGATTTCAGCTGGAACAACGAAAGTGGTTGCATTTGGGCCAAAATTTGCAAAGAAAAATGTTAGTGAG

Coding sequence (CDS)

ATGTCGTTCAGAGGCCTCCGACGGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACATTTTCTGAACTACAAAGAAAGAAAATGTATCGATATGTGATCTTTAAGGTCGATGAAAAGAAAAGAGAGGTGGTGGTTGACAAGATTGGCAATCCTGCAGAGAGCTATGAAGATTTCACTGCTGCTCTGCCCGACAATGATTGCCGCTACGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGCCAGAAGAGCAAGATATTTTTTATAGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTCGATGGCATCCACTATGAAATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATCAGAGACCGTGCTCAATGA

Protein sequence

MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEMDLEVIRDRAQ
Homology
BLAST of Lsi05G010150 vs. ExPASy Swiss-Prot
Match: Q8SAG3 (Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 8.0e-60
Identity = 112/145 (77.24%), Postives = 129/145 (88.97%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGL   N  S MGV +H+K TF EL+RKK++RYVIFK+DEKK+EVVV+K G PAES+
Sbjct: 1   MSFRGL---NASSGMGVADHSKNTFLELKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESF 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           ++F AALP+NDCRYAVYDFDFVTS+NCQKSKIFFIAWSP SSRIRAKMLYATSK+ FR E
Sbjct: 61  DEFAAALPENDCRYAVYDFDFVTSENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRA 146
           LDG+HYEIQATDP EMDLEV+R+RA
Sbjct: 121 LDGVHYEIQATDPTEMDLEVLRERA 142

BLAST of Lsi05G010150 vs. ExPASy Swiss-Prot
Match: Q9ZSK2 (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.3e-57
Identity = 106/145 (73.10%), Postives = 124/145 (85.52%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGL R N +S MGV + +K TF ELQRKK +RYV+FK+DE K+EVVV+K GNP ESY
Sbjct: 1   MSFRGLSRPNAISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           +DF A+LPDNDCRYAVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    E
Sbjct: 61  DDFLASLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRA 146
           L GIHYEIQATDP E+DLEV+R+RA
Sbjct: 121 LQGIHYEIQATDPTEVDLEVLRERA 145

BLAST of Lsi05G010150 vs. ExPASy Swiss-Prot
Match: Q2QLT8 (Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 5.2e-51
Identity = 93/138 (67.39%), Postives = 118/138 (85.51%), Query Frame = 0

Query: 8   RQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAAL 67
           R N  S +GV    K+TF ELQRKK +RYVIFK+D+K +EVVV+K G+  ES++DF  +L
Sbjct: 7   RANASSGIGVAAECKQTFLELQRKKSHRYVIFKIDDKCKEVVVEKTGSSTESFDDFMDSL 66

Query: 68  PDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYE 127
           P++DCRYA+YDFDFVT +NCQKSKIFF+AWSP+ SRIRAKMLYATSK+ FR ELDG+HYE
Sbjct: 67  PESDCRYAIYDFDFVTEENCQKSKIFFVAWSPSVSRIRAKMLYATSKERFRRELDGVHYE 126

Query: 128 IQATDPAEMDLEVIRDRA 146
           IQATDP+E+D+E++R+RA
Sbjct: 127 IQATDPSELDIELLRERA 144

BLAST of Lsi05G010150 vs. ExPASy Swiss-Prot
Match: Q9AY76 (Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.2e-49
Identity = 93/136 (68.38%), Postives = 113/136 (83.09%), Query Frame = 0

Query: 10  NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPD 69
           N  S MGV    + TF ELQ KK +RYVIFK++EK+++VVV+K G   ESY+DF A+LP+
Sbjct: 9   NASSGMGVAPDIRDTFLELQMKKAFRYVIFKIEEKQKQVVVEKTGATTESYDDFLASLPE 68

Query: 70  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 129
           NDCRYA+YDFDFVT +N QKSKIFFIAWSP++SRIRAKMLY+TSKD  + ELDG HYEIQ
Sbjct: 69  NDCRYALYDFDFVTGENVQKSKIFFIAWSPSTSRIRAKMLYSTSKDRIKQELDGFHYEIQ 128

Query: 130 ATDPAEMDLEVIRDRA 146
           ATDP E+DLEV+R+RA
Sbjct: 129 ATDPTEVDLEVLRERA 144

BLAST of Lsi05G010150 vs. ExPASy Swiss-Prot
Match: Q337A5 (Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.5e-47
Identity = 86/129 (66.67%), Postives = 111/129 (86.05%), Query Frame = 0

Query: 17  VNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRYAV 76
           V E +K  F EL+R+K++RYVIFK+D+++ E+VV+K G P ESY+DFTA+LP +DCRYAV
Sbjct: 20  VPEKSKSAFWELKRRKVHRYVIFKIDDRREEIVVEKTGAPGESYDDFTASLPADDCRYAV 79

Query: 77  YDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEM 136
           YD DFV+ DNC+KSKIFFI+WSP+ SRIRAK +YA S++ FRHELDG+H+EIQATDP +M
Sbjct: 80  YDLDFVSDDNCRKSKIFFISWSPSVSRIRAKTIYAVSRNQFRHELDGVHFEIQATDPDDM 139

Query: 137 DLEVIRDRA 146
           DLEV+R RA
Sbjct: 140 DLEVLRGRA 148

BLAST of Lsi05G010150 vs. ExPASy TrEMBL
Match: A0A1S3BHG1 (actin-depolymerizing factor OS=Cucumis melo OX=3656 GN=LOC103489879 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.8e-76
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. ExPASy TrEMBL
Match: A0A6J1K588 (actin-depolymerizing factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491296 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.8e-76
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. ExPASy TrEMBL
Match: A0A6J1EY99 (actin-depolymerizing factor-like OS=Cucurbita moschata OX=3662 GN=LOC111437311 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.8e-76
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. ExPASy TrEMBL
Match: A0A0A0L7Z2 (ADF-H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G389830 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 6.3e-76
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIF+VDEKKREVVVDKIGNPAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. ExPASy TrEMBL
Match: A0A6J1CY17 (actin-depolymerizing factor OS=Momordica charantia OX=3673 GN=LOC111015308 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.2e-74
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. NCBI nr
Match: XP_008447430.1 (PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_008447431.1 PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_022930980.1 actin-depolymerizing factor-like [Cucurbita moschata] >XP_022995915.1 actin-depolymerizing factor-like [Cucurbita maxima] >XP_023533852.1 actin-depolymerizing factor-like [Cucurbita pepo subsp. pepo] >KAG6606377.1 Actin-depolymerizing factor 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036318.1 Actin-depolymerizing factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 294.3 bits (752), Expect = 5.9e-76
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. NCBI nr
Match: XP_004151983.1 (actin-depolymerizing factor [Cucumis sativus] >XP_011651442.1 actin-depolymerizing factor [Cucumis sativus] >KGN57913.1 hypothetical protein Csa_010146 [Cucumis sativus])

HSP 1 Score: 293.1 bits (749), Expect = 1.3e-75
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIF+VDEKKREVVVDKIGNPAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. NCBI nr
Match: XP_038888227.1 (actin-depolymerizing factor [Benincasa hispida])

HSP 1 Score: 292.4 bits (747), Expect = 2.2e-75
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIG+PAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGSPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. NCBI nr
Match: XP_022145996.1 (actin-depolymerizing factor [Momordica charantia])

HSP 1 Score: 288.9 bits (738), Expect = 2.5e-74
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           LDGIHYEIQATDPAEMDLEVIRDRAQ
Sbjct: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 146

BLAST of Lsi05G010150 vs. NCBI nr
Match: XP_022963942.1 (actin-depolymerizing factor-like [Cucurbita moschata] >XP_022967676.1 actin-depolymerizing factor-like [Cucurbita maxima] >XP_023553738.1 actin-depolymerizing factor-like [Cucurbita pepo subsp. pepo] >KAG6571521.1 Actin-depolymerizing factor 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011266.1 Actin-depolymerizing factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.9 bits (725), Expect = 7.9e-73
Identity = 140/146 (95.89%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGLRRQNTLSAMGVNEHTKK FSELQRKKMYRYVIFKVDEKKREVVVDKIG+PAESY
Sbjct: 1   MSFRGLRRQNTLSAMGVNEHTKKIFSELQRKKMYRYVIFKVDEKKREVVVDKIGSPAESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKD+FRH+
Sbjct: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDSFRHD 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRAQ 147
           L+GIHYEIQATDPAEMDL+VIRDRAQ
Sbjct: 121 LNGIHYEIQATDPAEMDLDVIRDRAQ 146

BLAST of Lsi05G010150 vs. TAIR 10
Match: AT2G31200.1 (actin depolymerizing factor 6 )

HSP 1 Score: 223.8 bits (569), Expect = 9.1e-59
Identity = 106/145 (73.10%), Postives = 124/145 (85.52%), Query Frame = 0

Query: 1   MSFRGLRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESY 60
           MSFRGL R N +S MGV + +K TF ELQRKK +RYV+FK+DE K+EVVV+K GNP ESY
Sbjct: 1   MSFRGLSRPNAISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESY 60

Query: 61  EDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHE 120
           +DF A+LPDNDCRYAVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    E
Sbjct: 61  DDFLASLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRE 120

Query: 121 LDGIHYEIQATDPAEMDLEVIRDRA 146
           L GIHYEIQATDP E+DLEV+R+RA
Sbjct: 121 LQGIHYEIQATDPTEVDLEVLRERA 145

BLAST of Lsi05G010150 vs. TAIR 10
Match: AT4G00680.1 (actin depolymerizing factor 8 )

HSP 1 Score: 186.4 bits (472), Expect = 1.6e-47
Identity = 81/135 (60.00%), Postives = 113/135 (83.70%), Query Frame = 0

Query: 10  NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPD 69
           N+ S M VN+  K  F EL+ K+ YR+++FK+DEK ++V ++K+GNP E+Y+DFT+++PD
Sbjct: 3   NSASGMHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPD 62

Query: 70  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 129
           ++CRYAVYDFDF T DNCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ E++GI  E+Q
Sbjct: 63  DECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVELQ 122

Query: 130 ATDPAEMDLEVIRDR 145
           ATDP+EM L++I+ R
Sbjct: 123 ATDPSEMSLDIIKGR 137

BLAST of Lsi05G010150 vs. TAIR 10
Match: AT1G01750.1 (actin depolymerizing factor 11 )

HSP 1 Score: 185.3 bits (469), Expect = 3.6e-47
Identity = 81/135 (60.00%), Postives = 113/135 (83.70%), Query Frame = 0

Query: 10  NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPD 69
           N+ S M V++  K  F EL+ K+ YR+++FK+DEK ++V++DK+GNP E+YEDFT ++P+
Sbjct: 3   NSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE 62

Query: 70  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 129
           ++CRYAVYD+DF T +NCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ ELDGI  E+Q
Sbjct: 63  DECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQ 122

Query: 130 ATDPAEMDLEVIRDR 145
           ATDP+EM L++I+ R
Sbjct: 123 ATDPSEMSLDIIKGR 137

BLAST of Lsi05G010150 vs. TAIR 10
Match: AT4G25590.1 (actin depolymerizing factor 7 )

HSP 1 Score: 178.3 bits (451), Expect = 4.4e-45
Identity = 82/136 (60.29%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 10  NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPD 69
           N  S M V +  K  F EL+ K+ YR++IF++D   ++VVV+K+GNP E+Y+DFTA+LP 
Sbjct: 3   NAASGMAVEDECKLKFLELKSKRNYRFIIFRID--GQQVVVEKLGNPDETYDDFTASLPA 62

Query: 70  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 129
           N+CRYAV+DFDF+T +NCQKSKIFFIAWSP SSR+R KM+YA+SKD F+ ELDGI  E+Q
Sbjct: 63  NECRYAVFDFDFITDENCQKSKIFFIAWSPDSSRVRMKMVYASSKDRFKRELDGIQVELQ 122

Query: 130 ATDPAEMDLEVIRDRA 146
           ATDP+EM  ++I+ RA
Sbjct: 123 ATDPSEMSFDIIKSRA 136

BLAST of Lsi05G010150 vs. TAIR 10
Match: AT3G46010.2 (actin depolymerizing factor 1 )

HSP 1 Score: 177.9 bits (450), Expect = 5.7e-45
Identity = 78/139 (56.12%), Postives = 111/139 (79.86%), Query Frame = 0

Query: 7   RRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAA 66
           R  N  S M V++  K  F EL+ K+ +R++++K++EK+++VVV+K+G P ++YE+F A 
Sbjct: 11  RSANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAAC 70

Query: 67  LPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHY 126
           LP ++CRYA+YDFDFVT++NCQKSKIFFIAW P  +++R+KM+YA+SKD F+ ELDGI  
Sbjct: 71  LPADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQV 130

Query: 127 EIQATDPAEMDLEVIRDRA 146
           E+QATDP EMDL+V R RA
Sbjct: 131 ELQATDPTEMDLDVFRSRA 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8SAG38.0e-6077.24Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q9ZSK21.3e-5773.10Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1[more]
Q2QLT85.2e-5167.39Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11... [more]
Q9AY762.2e-4968.38Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 P... [more]
Q337A53.5e-4766.67Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10... [more]
Match NameE-valueIdentityDescription
A0A1S3BHG12.8e-76100.00actin-depolymerizing factor OS=Cucumis melo OX=3656 GN=LOC103489879 PE=3 SV=1[more]
A0A6J1K5882.8e-76100.00actin-depolymerizing factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491296 PE=... [more]
A0A6J1EY992.8e-76100.00actin-depolymerizing factor-like OS=Cucurbita moschata OX=3662 GN=LOC111437311 P... [more]
A0A0A0L7Z26.3e-7699.32ADF-H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G389830 PE=3 ... [more]
A0A6J1CY171.2e-7497.95actin-depolymerizing factor OS=Momordica charantia OX=3673 GN=LOC111015308 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_008447430.15.9e-76100.00PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_008447431.1 PREDICTED:... [more]
XP_004151983.11.3e-7599.32actin-depolymerizing factor [Cucumis sativus] >XP_011651442.1 actin-depolymerizi... [more]
XP_038888227.12.2e-7599.32actin-depolymerizing factor [Benincasa hispida][more]
XP_022145996.12.5e-7497.95actin-depolymerizing factor [Momordica charantia][more]
XP_022963942.17.9e-7395.89actin-depolymerizing factor-like [Cucurbita moschata] >XP_022967676.1 actin-depo... [more]
Match NameE-valueIdentityDescription
AT2G31200.19.1e-5973.10actin depolymerizing factor 6 [more]
AT4G00680.11.6e-4760.00actin depolymerizing factor 8 [more]
AT1G01750.13.6e-4760.00actin depolymerizing factor 11 [more]
AT4G25590.14.4e-4560.29actin depolymerizing factor 7 [more]
AT3G46010.25.7e-4556.12actin depolymerizing factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002108Actin-depolymerising factor homology domainSMARTSM00102adf_2coord: 19..146
e-value: 9.2E-53
score: 191.3
IPR002108Actin-depolymerising factor homology domainPFAMPF00241Cofilin_ADFcoord: 22..143
e-value: 8.9E-38
score: 128.9
IPR002108Actin-depolymerising factor homology domainPROSITEPS51263ADF_Hcoord: 13..146
score: 39.007858
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 5..146
e-value: 3.1E-50
score: 172.1
IPR017904ADF/CofilinPANTHERPTHR11913COFILIN-RELATEDcoord: 1..146
IPR017904ADF/CofilinCDDcd11286ADF_cofilin_likecoord: 13..145
e-value: 6.22961E-61
score: 181.986
NoneNo IPR availablePANTHERPTHR11913:SF96COFILIN/ACTIN-DEPOLYMERIZING FACTOR-LIKE PROTEINcoord: 1..146
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 10..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G010150.1Lsi05G010150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030042 actin filament depolymerization
cellular_component GO:0015629 actin cytoskeleton
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051015 actin filament binding
molecular_function GO:0003779 actin binding