Lsi05G009680 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G009680
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	beta-carotene isomerase D27, chloroplastic
Location	chr05: 16884944 .. 16889743 (-)
RNA-Seq Expression	Lsi05G009680
Synteny	Lsi05G009680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAATATGTATATATATGTATTTCTTCTCCATTCCCATCTCTATTCCCATAATTCAAACGACCTTACTTCTCTTAAAGCCCCTCTATCTTTAAATACAAGCAAAGTTTGTTGTAAGCCTAAAATCTCCCTCCCTTCTCATCTCTATCTCAAAACAAAGAAATGGATTTAAAAGTTATTCAAGAAAGGAGAGTAATTTTGCCAACAATGATGAACAACAACAACAAAATGAAGAAGTTAAGAAGAAAAAGCATTCCTTTTGTTGCAGTGCTCACAAGATCACCAGCAGAGAGTGGAGTAATAGAAGAACCAAGAAAAACCAATACTGTTTATACAGATAATTGGTTTGATAAAATAGCCATAGGCCATCTGTCTCAGGCAGTTCAAGCAACATCAGGTTGATTTTATTTATTTATTTTTTTTTTTTTTTGGAAGATTTGGAAAAAGGGAGGGGAAAAGAAGGTAAAATATAGAATAATGATGATATGCAGGTTGGAGAAGCAAAAAGAGTGGATATGAAAGCTTATTGGAAGTGACAACAATGGCATCAAGAAATTTCAACCAGATCAAACAAAGAGAAGTTGTCATTCAAGCCCTTGGAAGGGCATTTCCAAAGCCAATCCTCTCTTTGGTAAACTTCATTCATTGCCATTTAAACACCATTATGTTTCATTCATTACCATTTAATACCATTTACTATTCTTTTAGCTTTCTACCCTTCCTTCTCTTTCATCATCTATGTATTATATATGCATATCATGTATATATGTAAAATACCTATTACCAAACCTATCATTATAAAGATGTCAAAATCAGCTTAACTCAACTAGTATAAGTATATGTTGACAATCAAGAGGTTTGTGGTTCGAATCTCTCACCTTTATAGTACTAAAAAGAACATATCATTAAAGATTATGAATAGTAATTTCAAACTTACATTAACTTTTACTTTGACCAATGATACAAATATTATTGTTAAGAGTCATTTAAAAATAGAAAAAAACTTAGGTACACCCATCTTTTGTGCAACCTAACTAGCACTCAATAGGAATATGTCACGTGGAAGTTTTGTTTTTTTTTTTTTTTTTTTTAAATTTTAAATAAAAAGGAGGGGAGTATGTGATTAGGTGCAGCCTAATTGTTGCTCTTTAAAAATCACATCTTTTCAGGTGGCTCTAAGTTTTTTATTTTTTAGTACAACTTAAGAGAGGGGGGCATGCTAATTTTTGTTGAACTAAACTCACTTTGGCATCCTAAATTATTTATTTACCTATGATATTGTACATTATTATTAAATAATTAACTTAGGGTAAGTAATGCATGATCATTTCATCCTTGATGAATTTTATTTGGGCCAAATTTTTCAACACTTGTCTTCCTCATTTGGTCTCTCTTCTCCCATCAATATATGGGGGAATATAGCTAGGATTCATTGCCTAGGTTAGAACCTACTGAAGAACTATACCCTTTTCTAATTTTTCTTTCAACTAATCATTGGGTTTAATAATTATTTGATTGTCACGTCATTATATGGAAATAGTATAATCAAATGGTCACATTTTCTTATGTTAGTTTTATTATGGGTAATTTTTCGATTAAACAAGTATTAAAATTGTACTTTTACTATTATTAATCCATCTCCAATTCGCATTTTACTATTATACCCTCACTCCTCTCAACTATATGTCCTTAACATATAGTGGTGAACAAAGTGCAGATAAAAGCATTGCTACCACAATCCAAATTCGCAAGGGAATATTTTGCTGTATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTAAGATGTTGTAAATTTACCCAAAGCTCTTTATTTCCTCAGTATGTTCATATCAATCCAAGGACCTAATTGGGAATTCACTTGAATACATACATAAATACACACGAGAAAGAGACTAATCTTGACATAACTCAACTGTTAAGGCATATGTCACTGAATAAAAGGTTAAAGGTTTGTGAAACCCTCATTTCACCGAGTATGTAATGAAAGATAGAGACTTACTTGAGTACAACTCAACTATTAATACATAAATCATCAATCGGGAGGTCAGAAGTTTGAATCTTCTACCCTCCGGTTGAATTCAACCGAGAGAGAGCCTTTTCTTTCTTTTGTATTGGAAAACTAAATAAGTGCTCAACACCACCATTTAGGACCGATGTCAAAGAGTTTGCTGAGAATAGACAGTATTTAAACGGAGGACATTGAACAACCAAGGTCAGAATTAGGAAAGATTCACAAGGTTGAAAAGAGTGACATATGAGGAGGCATAATGGTGGACATCAAGAACTTCACCTTTAGCACTAAAATGAATATAATAATATGATTTTCAATGGCCAAGTTGATAAACCTGCAGTGTTTGAGGCACTAGTCCCTTCTAGACTTGTCCTGTCCCTACAAATCAAAGAAAGGTTTTTGTGACGTGTAAGATATGTGAACCAATTCTTTGAGAAACTATCTTCTCTTCATGCTTACTCACTTAAGCCCTTTCAGTTTCTAGGCATTCCTCACGAATTCAGATTAATTACAGCTGATATGTCTAGATTGTTAGGGAGGGAGGGATTGAAAAGGCACACACATGGAAGAAACAAGTCTTTTTTTTCGATCACTAATTCATAAGACTGACAATAGTAACATATGATTCTTACAGGTGAAGGAATCAGAATTCGAAGGGAAAATAGAAAAAAATGTGGTTCAAATCCATAAGTGCAGGTAATTCTTTCTCGCATCAGAGTTCTATAATTAAGGCAATTTTATCTAACCGTTCATGGGGTCCCATTCTCACCTACCTGAGACACTTTCCACACCCACAGAACTATCTGTCTCTAATTATTAGACTTCTTCTAAACCCTCAATACTTAATCACATTAAGTGATGTCCATAATTTAGATCATTGAAAATTTGAAATTATCTTAGTAAAAAATAACATTCAACAAATCTATATTGCCCCTAAACATATCCCATGTATTTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATAAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAGTAAGTCTCCTCACTGAACTACAGAATCACCAAACAATTGCAGCAACCATATATTGTTTTCCCAAAAGAAAAACAAATTTCAACTTTTCAAAAAGAAAAAAAAAATTAAAAAATAAAATCGCATATACTGCAGCACAAAGCAATCCTGGACACTAATTCAAGCTTCCAAACTATAACGGAAGGCCATAATTTGTTTTTCTCATGTTCATAGTGTTTAGAGTTTGTTTGCACACGCCTCTACTATTCTTATAAGACAACCCTACAATATTTGGTTGCCGAGAAAAATCATAGAAAATTAAATCATAGCTAGCCATATGGCTTGAGCTCATTCCTTCTATACTTTTTATGAGCATTAAGCCAACCTATGGTCAATGGTGGTTAACTCGAGGCCATTATAAAAATTTCATATTCACTTAAAGAGGAATATTAGAATTAAATTATTTCAGCTGAATCTATATGGTCTATATGTGAGGACACTAAATAAAGTAAATTAGCTATAGTGCAATGACCAACGACTGGTAGATCCATGAGCAAAAGTGAAATTACTATTAAAGCAACATTACCAGCACCAAATGGTGGAACGGATCATGTTTGTCAATGTGAATGGAGGGTCCCATGCACGAACCATTTTGGGTACAAAAGAACTGCAGATAGGCCCCATCCAAGCTAAGTGGGATTTCAAACCTGCAGCTTAATTGTGCAATATGGTTATAATCCAAGAATTAAAAGCTGAATTTCCATCGTTAATCAATCATCAAGCTGAATTTCCCACTGAGCCAATCATCGCTCTTGCCTATGATATACATTAATCATAACAAGTGTAAAAAATGCAACCAAGCAGAGGCATTAGTATTAGGATGTTAATTGTGCACCATGATGTAACTACTAGCTGGGAACCATTAATATTTATGTCAAGGACCAAGAAATACATCAATATGCAAGCGGAATTTGGGAGCAAAAAAGAGGGGAGGAGGGGGGAAGAAGCTTTATAATATGATTCAAATAACTGTCTTTTTCTTTGCAGATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCAGCTCCAATCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGTATTAACCTTAACACAAGTCTCTTCTTCTTCCTTTTCTTATTATTACCTTTTACTTTACCTGTGAGTGGAGAAGTCAAACATATTCAAATAATTCACTGGGAAAGGTAGCAGGACTTTAGGTCCAAACGAGATTTTTAAGGGGATAGTAGTATAAGTTAAAAATGATTCTAATCTTATTTGTTTACTTTTTGCAGGCAAAACAAAAGAGAAACATACGACAAACTGCTTCATTTAGAGCAAAGGGACGCTGAAAGAATTTTTTTGGATGAGATCCATTTTACCTCCCAATTGTTTTTGTTTCCAATATTTTCTGGATTACAGGAGAACCTCTTTTCTTCTTCCAAGATGAACAGATAGTTTTACTGTTTAGGCATTAGAGCAAAAATAACTTTGCAGGAATCTGGCTTTCTGGTCATTATATTGGGTCAAAGATGAAATCTACAATACTGTCAGATAACATGAATACAATTAAAAGCATGTGTATAGGAAAAGGGTTAAGATGCAGTAATTACAATCGATTATCCTCACGGGAAATGCTAAAATGGACAATATATTTCATAGTGCCTTATTAGAAA

mRNA sequence

TAATATGTATATATATGTATTTCTTCTCCATTCCCATCTCTATTCCCATAATTCAAACGACCTTACTTCTCTTAAAGCCCCTCTATCTTTAAATACAAGCAAAGTTTGTTGTAAGCCTAAAATCTCCCTCCCTTCTCATCTCTATCTCAAAACAAAGAAATGGATTTAAAAGTTATTCAAGAAAGGAGAGTAATTTTGCCAACAATGATGAACAACAACAACAAAATGAAGAAGTTAAGAAGAAAAAGCATTCCTTTTGTTGCAGTGCTCACAAGATCACCAGCAGAGAGTGGAGTAATAGAAGAACCAAGAAAAACCAATACTGTTTATACAGATAATTGGTTTGATAAAATAGCCATAGGCCATCTGTCTCAGGCAGTTCAAGCAACATCAGGTTGGAGAAGCAAAAAGAGTGGATATGAAAGCTTATTGGAAGTGACAACAATGGCATCAAGAAATTTCAACCAGATCAAACAAAGAGAAGTTGTCATTCAAGCCCTTGGAAGGGCATTTCCAAAGCCAATCCTCTCTTTGATAAAAGCATTGCTACCACAATCCAAATTCGCAAGGGAATATTTTGCTGTATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTGAAGGAATCAGAATTCGAAGGGAAAATAGAAAAAAATGTGGTTCAAATCCATAAGTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATAAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCAGCTCCAATCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGCAAAACAAAAGAGAAACATACGACAAACTGCTTCATTTAGAGCAAAGGGACGCTGAAAGAATTTTTTTGGATGAGATCCATTTTACCTCCCAATTGTTTTTGTTTCCAATATTTTCTGGATTACAGGAGAACCTCTTTTCTTCTTCCAAGATGAACAGATAGTTTTACTGTTTAGGCATTAGAGCAAAAATAACTTTGCAGGAATCTGGCTTTCTGGTCATTATATTGGGTCAAAGATGAAATCTACAATACTGTCAGATAACATGAATACAATTAAAAGCATGTGTATAGGAAAAGGGTTAAGATGCAGTAATTACAATCGATTATCCTCACGGGAAATGCTAAAATGGACAATATATTTCATAGTGCCTTATTAGAAA

Coding sequence (CDS)

ATGGATTTAAAAGTTATTCAAGAAAGGAGAGTAATTTTGCCAACAATGATGAACAACAACAACAAAATGAAGAAGTTAAGAAGAAAAAGCATTCCTTTTGTTGCAGTGCTCACAAGATCACCAGCAGAGAGTGGAGTAATAGAAGAACCAAGAAAAACCAATACTGTTTATACAGATAATTGGTTTGATAAAATAGCCATAGGCCATCTGTCTCAGGCAGTTCAAGCAACATCAGGTTGGAGAAGCAAAAAGAGTGGATATGAAAGCTTATTGGAAGTGACAACAATGGCATCAAGAAATTTCAACCAGATCAAACAAAGAGAAGTTGTCATTCAAGCCCTTGGAAGGGCATTTCCAAAGCCAATCCTCTCTTTGATAAAAGCATTGCTACCACAATCCAAATTCGCAAGGGAATATTTTGCTGTATTCACCACTGTCTTCTTTGCTTGGTTAGTTGGACCCTGCGAGGTGAAGGAATCAGAATTCGAAGGGAAAATAGAAAAAAATGTGGTTCAAATCCATAAGTGCAGGTTCCTAGAGCAAACTAACTGTGCTGGAATGTGCATTAATCTATGCAAGTTTCCATGTCAGGATTTCATAAAGGATTCTTTGGGAATGCCAGTCAGCATGGTCCCCAATTTTGATGACATGAGCTGTGAGATGATATTTGGAAAGGATCCACCAGCTCCAATCGATGATCCAGCGCTTAAGCAACCATGTTACAAACTATGCAAAACAAAAGAGAAACATACGACAAACTGCTTCATTTAG

Protein sequence

MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDNWFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPCYKLCKTKEKHTTNCFI

Homology

[Close]

BLAST of Lsi05G009680 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.7e-84
Identity = 155/255 (60.78%), Postives = 190/255 (74.51%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRK-SIPFVAVLTRSPAESGVIEEPRKTNTVYTD 60
           MD K+I     + PT+     + KKLR K    FV  +   P +    E  RKTN VY D
Sbjct: 1   MDSKMIAHNMSLTPTLA----QWKKLRLKPKHTFVVGVLARPTDDISEETLRKTN-VYKD 60

Query: 61  NWFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFP 120
           NWFDK+AI HLS++VQA +G  + KSG++SL+E  T+AS+ FN  +Q+ +++ AL RAFP
Sbjct: 61  NWFDKLAINHLSKSVQAATGISNNKSGFDSLVEAATVASQKFNTTQQQGIILDALDRAFP 120

Query: 121 KPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFL 180
           KPILS+I+ ++P SK AREYFAVFTT+FFAWL+GP EV+ESE  G+ EKN+V I KCRFL
Sbjct: 121 KPILSVIRRVMPPSKLAREYFAVFTTIFFAWLLGPSEVRESEINGRREKNIVHIKKCRFL 180

Query: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQP 240
           E+TNC GMCINLCK P Q FIKDS GMPV+MVPNFDDMSCEMIFG++PPA  DDPALKQP
Sbjct: 181 EETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNFDDMSCEMIFGQEPPASTDDPALKQP 240

Query: 241 CYKLCKTKEKHTTNC 255
           CYKLCK K+ H T C
Sbjct: 241 CYKLCKAKKNHATQC 250

[Close]

BLAST of Lsi05G009680 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 7.9e-71
Identity = 128/203 (63.05%), Postives = 154/203 (75.86%), Query Frame = 0


Query: 55  TVYTDNWFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQAL 114
           TVY D+WFDK+AIG+LS+ +Q  SG +++K GYESL++     SR F+  KQ E+V QAL
Sbjct: 78  TVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQAL 137

Query: 115 GRAFPKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIH 174
            RA P  IL++IK ++P S+F+REYFA FTT+FF WLVGPCEV ESE EG+ EKNVV I 
Sbjct: 138 ERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIP 197

Query: 175 KCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDP 234
           KCRFLE TNC GMC NLCK PCQ FI+DSLGM V M PNF+DMSCEMIFG+ PP   DDP
Sbjct: 198 KCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPE--DDP 257

Query: 235 ALKQPCYKL-CKTKEKHTTNCFI 257
           ALKQPC++  C  K+ H  NC I
Sbjct: 258 ALKQPCFRTKCVAKQNHGVNCSI 278

[Close]

BLAST of Lsi05G009680 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 8.5e-57
Identity = 114/254 (44.88%), Postives = 163/254 (64.17%), Query Frame = 0


Query: 6   IQERRVILPTMMNNNNKMKKLRRKSI-------PFVAVLTRSPAESGVIEEPRKTNTVYT 65
           + + ++   T   N+ +    RR SI       P  +   ++  E+  IE     N    
Sbjct: 7   LSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNTKNASIE 66

Query: 66  DNWFDKIAIGHLSQAVQATSG--WRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGR 125
           D++F KIAI +LS+ +Q  +G    SK + Y+ L++  T  SRNF+  +Q E V+ +L R
Sbjct: 67  DSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFVLSSLDR 126

Query: 126 AFPKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKC 185
           A P  I SLIK   P SK +RE FA+FTT+ FAWLVGP EV+E+E  G+ EK+VV I KC
Sbjct: 127 ALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKC 186

Query: 186 RFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPAL 245
           RFLEQ+NC GMC ++CK P Q FIK+SLGMP+ M P+F+D+SC+M+FG++PP   DDPA+
Sbjct: 187 RFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAM 246

Query: 246 KQPCYKLCKTKEKH 251
           KQPC++ CK+ + +
Sbjct: 247 KQPCFEFCKSNKSY 260

[Close]

BLAST of Lsi05G009680 vs. ExPASy TrEMBL
Match: A0A5D3E3G1 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00920 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 9.0e-126
Identity = 230/256 (89.84%), Postives = 237/256 (92.58%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQ+  VILPTMMNNN    KLRRK+I  V VLTRS AE  VIEE RKT TVYTDN
Sbjct: 1   MDLRVIQDTTVILPTMMNNNK--MKLRRKNILCVGVLTRS-AEGEVIEETRKTKTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESLLEVTTMASRNFN IKQ+EVVIQALGRAFPK
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNHIKQKEVVIQALGRAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKALLPQSKFAREYFA FTTVFFAWLVGPCEVKESEF+GK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPV+MVPNFDDMSCEMIFGK+PPA IDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCKTKEKHTTNCFI
Sbjct: 241 YKLCKTKEKHTTNCFI 253

[Close]

BLAST of Lsi05G009680 vs. ExPASy TrEMBL
Match: A0A1S3BL84 (LOW QUALITY PROTEIN: beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491262 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.6e-125
Identity = 229/256 (89.45%), Postives = 236/256 (92.19%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQ+  VILPTMMNNN    KLRRK+   V VLTRS AE  VIEE RKT TVYTDN
Sbjct: 1   MDLRVIQDTTVILPTMMNNNK--MKLRRKTFFXVGVLTRS-AEGEVIEETRKTKTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESLLEVTTMASRNFN IKQ+EVVIQALGRAFPK
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNHIKQKEVVIQALGRAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKALLPQSKFAREYFA FTTVFFAWLVGPCEVKESEF+GK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPV+MVPNFDDMSCEMIFGK+PPA IDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCKTKEKHTTNCFI
Sbjct: 241 YKLCKTKEKHTTNCFI 253

[Close]

BLAST of Lsi05G009680 vs. ExPASy TrEMBL
Match: A0A0A0LIR1 (DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G226860 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.4e-125
Identity = 228/256 (89.06%), Postives = 236/256 (92.19%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQ+ RVILPTMMNNN    KLRRKSI    VLTR PAE  +IEE RKTNTVYTDN
Sbjct: 1   MDLRVIQDTRVILPTMMNNNK--MKLRRKSILCFGVLTR-PAEGELIEETRKTNTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESL+EVTTMASRNFN IKQ+EVVIQALG AFPK
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLVEVTTMASRNFNHIKQKEVVIQALGMAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKALLPQSK AREYFA FTTVFFAWLVGPCEVKESEF+GK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALLPQSKLAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPV+MVPNFDDMSCEMIFGK+PPA IDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCKTKEKHTTNCFI
Sbjct: 241 YKLCKTKEKHTTNCFI 253

[Close]

BLAST of Lsi05G009680 vs. ExPASy TrEMBL
Match: A0A6J1HUP9 (beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111466998 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 3.9e-121
Identity = 219/258 (84.88%), Postives = 236/258 (91.47%), Query Frame = 0


Query: 1   MDLKVIQERRVIL--PTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYT 60
           M+++V +ERRVIL   T +  +NK KK+RRKSI  VAVLTR+ AESG  EE RK NTVYT
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRA-AESGATEETRKPNTVYT 60

Query: 61  DNWFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAF 120
           DNWF+KIAI H+SQAVQATSGWRSKKSGYESL+EV TMASRN+NQIKQREV+IQALGRAF
Sbjct: 61  DNWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAF 120

Query: 121 PKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRF 180
           P PILSLI+ LLPQSKFAREYFA FTTVFFAWLVGPCEVKES+F+GK EKNVVQIHKCRF
Sbjct: 121 PAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRF 180

Query: 181 LEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQ 240
           LEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPA +DDPALKQ
Sbjct: 181 LEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQ 240

Query: 241 PCYKLCKTKEKHTTNCFI 257
           PCYKLCKTKEKHTTNCFI
Sbjct: 241 PCYKLCKTKEKHTTNCFI 257

[Close]

BLAST of Lsi05G009680 vs. ExPASy TrEMBL
Match: A0A6J1HHC5 (beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111464069 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 9.6e-120
Identity = 217/258 (84.11%), Postives = 234/258 (90.70%), Query Frame = 0


Query: 1   MDLKVIQERRVIL--PTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYT 60
           M+++V + RRVIL   T +  +NK KK+RRKSI  VAVLTR+ AESG  EE RK NTVYT
Sbjct: 1   MEVRVGEVRRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRA-AESGATEETRKPNTVYT 60

Query: 61  DNWFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAF 120
           DNWF+KIAI H+SQAVQATSGWRS KSGYESL+EV TMASRN+NQIKQREV+IQALGRAF
Sbjct: 61  DNWFEKIAIHHVSQAVQATSGWRSNKSGYESLVEVATMASRNYNQIKQREVIIQALGRAF 120

Query: 121 PKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRF 180
           P PILSLI+ LLPQSKFAREYFA FTTVFFAWLVGPCEVKES+F+GK EKNVVQIHKCRF
Sbjct: 121 PAPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKREKNVVQIHKCRF 180

Query: 181 LEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQ 240
           LEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPA +DDPALKQ
Sbjct: 181 LEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQ 240

Query: 241 PCYKLCKTKEKHTTNCFI 257
           PCYKLCKTKEKHTTNCFI
Sbjct: 241 PCYKLCKTKEKHTTNCFI 257

[Close]

BLAST of Lsi05G009680 vs. NCBI nr
Match: XP_038888067.1 (beta-carotene isomerase D27, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 490.3 bits (1261), Expect = 9.8e-135
Identity = 244/256 (95.31%), Postives = 249/256 (97.27%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQERRVILPTMM NNNKMKKL RKS+P VAVLTR PAESGVIEE RKTNTVYTDN
Sbjct: 1   MDLRVIQERRVILPTMM-NNNKMKKLGRKSLPCVAVLTR-PAESGVIEETRKTNTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESL+EVTTMASRNFNQIKQREVVIQALGRAFPK
Sbjct: 61  WFDKIAIHHLSQAVQATSGWRSKKSGYESLVEVTTMASRNFNQIKQREVVIQALGRAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKAL+PQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALVPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCK+KEKHTTNCFI
Sbjct: 241 YKLCKSKEKHTTNCFI 254

[Close]

BLAST of Lsi05G009680 vs. NCBI nr
Match: XP_038888066.1 (beta-carotene isomerase D27, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 465.3 bits (1196), Expect = 3.4e-127
Identity = 233/244 (95.49%), Postives = 237/244 (97.13%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQERRVILPTMM NNNKMKKL RKS+P VAVLTR PAESGVIEE RKTNTVYTDN
Sbjct: 1   MDLRVIQERRVILPTMM-NNNKMKKLGRKSLPCVAVLTR-PAESGVIEETRKTNTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESL+EVTTMASRNFNQIKQREVVIQALGRAFPK
Sbjct: 61  WFDKIAIHHLSQAVQATSGWRSKKSGYESLVEVTTMASRNFNQIKQREVVIQALGRAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKAL+PQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALVPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240

Query: 241 YKLC 245
           YKLC
Sbjct: 241 YKLC 242

[Close]

BLAST of Lsi05G009680 vs. NCBI nr
Match: KAA0057411.1 (beta-carotene isomerase D27 [Cucumis melo var. makuwa] >TYK30110.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa])

HSP 1 Score: 459.5 bits (1181), Expect = 1.9e-125
Identity = 230/256 (89.84%), Postives = 237/256 (92.58%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQ+  VILPTMMNNN    KLRRK+I  V VLTRS AE  VIEE RKT TVYTDN
Sbjct: 1   MDLRVIQDTTVILPTMMNNNK--MKLRRKNILCVGVLTRS-AEGEVIEETRKTKTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESLLEVTTMASRNFN IKQ+EVVIQALGRAFPK
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNHIKQKEVVIQALGRAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKALLPQSKFAREYFA FTTVFFAWLVGPCEVKESEF+GK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPV+MVPNFDDMSCEMIFGK+PPA IDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCKTKEKHTTNCFI
Sbjct: 241 YKLCKTKEKHTTNCFI 253

[Close]

BLAST of Lsi05G009680 vs. NCBI nr
Match: XP_008449360.1 (PREDICTED: LOW QUALITY PROTEIN: beta-carotene isomerase D27, chloroplastic [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 5.4e-125
Identity = 229/256 (89.45%), Postives = 236/256 (92.19%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQ+  VILPTMMNNN    KLRRK+   V VLTRS AE  VIEE RKT TVYTDN
Sbjct: 1   MDLRVIQDTTVILPTMMNNNK--MKLRRKTFFXVGVLTRS-AEGEVIEETRKTKTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESLLEVTTMASRNFN IKQ+EVVIQALGRAFPK
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNHIKQKEVVIQALGRAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKALLPQSKFAREYFA FTTVFFAWLVGPCEVKESEF+GK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPV+MVPNFDDMSCEMIFGK+PPA IDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCKTKEKHTTNCFI
Sbjct: 241 YKLCKTKEKHTTNCFI 253

[Close]

BLAST of Lsi05G009680 vs. NCBI nr
Match: XP_004145371.1 (beta-carotene isomerase D27, chloroplastic [Cucumis sativus] >KGN61698.1 hypothetical protein Csa_006159 [Cucumis sativus])

HSP 1 Score: 457.6 bits (1176), Expect = 7.0e-125
Identity = 228/256 (89.06%), Postives = 236/256 (92.19%), Query Frame = 0


Query: 1   MDLKVIQERRVILPTMMNNNNKMKKLRRKSIPFVAVLTRSPAESGVIEEPRKTNTVYTDN 60
           MDL+VIQ+ RVILPTMMNNN    KLRRKSI    VLTR PAE  +IEE RKTNTVYTDN
Sbjct: 1   MDLRVIQDTRVILPTMMNNNK--MKLRRKSILCFGVLTR-PAEGELIEETRKTNTVYTDN 60

Query: 61  WFDKIAIGHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGRAFPK 120
           WFDKIAI HLSQAVQATSGWRSKKSGYESL+EVTTMASRNFN IKQ+EVVIQALG AFPK
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLVEVTTMASRNFNHIKQKEVVIQALGMAFPK 120

Query: 121 PILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKCRFLE 180
           PILSLIKALLPQSK AREYFA FTTVFFAWLVGPCEVKESEF+GK EKNVVQIHKCRFLE
Sbjct: 121 PILSLIKALLPQSKLAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPC 240
           QTNCAGMCINLCKFPCQDFIKDSLGMPV+MVPNFDDMSCEMIFGK+PPA IDDPALKQPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YKLCKTKEKHTTNCFI 257
           YKLCKTKEKHTTNCFI
Sbjct: 241 YKLCKTKEKHTTNCFI 253

[Close]

BLAST of Lsi05G009680 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 221.9 bits (564), Expect = 6.1e-58
Identity = 114/254 (44.88%), Postives = 163/254 (64.17%), Query Frame = 0


Query: 6   IQERRVILPTMMNNNNKMKKLRRKSI-------PFVAVLTRSPAESGVIEEPRKTNTVYT 65
           + + ++   T   N+ +    RR SI       P  +   ++  E+  IE     N    
Sbjct: 7   LSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNTKNASIE 66

Query: 66  DNWFDKIAIGHLSQAVQATSG--WRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGR 125
           D++F KIAI +LS+ +Q  +G    SK + Y+ L++  T  SRNF+  +Q E V+ +L R
Sbjct: 67  DSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFVLSSLDR 126

Query: 126 AFPKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKC 185
           A P  I SLIK   P SK +RE FA+FTT+ FAWLVGP EV+E+E  G+ EK+VV I KC
Sbjct: 127 ALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKC 186

Query: 186 RFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPAL 245
           RFLEQ+NC GMC ++CK P Q FIK+SLGMP+ M P+F+D+SC+M+FG++PP   DDPA+
Sbjct: 187 RFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAM 246

Query: 246 KQPCYKLCKTKEKH 251
           KQPC++ CK+ + +
Sbjct: 247 KQPCFEFCKSNKSY 260

[Close]

BLAST of Lsi05G009680 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 149.8 bits (377), Expect = 2.9e-36
Identity = 74/199 (37.19%), Postives = 114/199 (57.29%), Query Frame = 0


Query: 50  PRKTNTVYTDNWFDKIAIGHLSQAVQATSGWRSKKS--------GYESLLEVTTMASRNF 109
           P    T Y D   +++ +G  ++ +      + K +         YES +EV+    +  
Sbjct: 32  PMGLKTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKEKGFWEYDYESFVEVSKRVMQGR 91

Query: 110 NQIKQREVVIQALGRAFPKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESE 169
           ++++Q+E V + L    P       + L P +K+A E+ A  T  FF WLVGP +V E E
Sbjct: 92  SRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAAEFNAALTVPFFHWLVGPSQVIEVE 151

Query: 170 FEGKIEKNVVQIHKCRFLEQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEM 229
             G  +++ V+I KCR+LE + C GMC+N+CK P QDF  +  G+P++M PN++DMSCEM
Sbjct: 152 VNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNYEDMSCEM 211

Query: 230 IFGKDPPAPIDDPALKQPC 241
           I+G+ PPA  +D A KQPC
Sbjct: 212 IYGQAPPAFEEDVATKQPC 230

[Close]

BLAST of Lsi05G009680 vs. TAIR 10
Match: AT1G03055.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 119.4 bits (298), Expect = 4.2e-27
Identity = 72/181 (39.78%), Postives = 103/181 (56.91%), Query Frame = 0


Query: 6   IQERRVILPTMMNNNNKMKKLRRKSI-------PFVAVLTRSPAESGVIEEPRKTNTVYT 65
           + + ++   T   N+ +    RR SI       P  +   ++  E+  IE     N    
Sbjct: 7   LSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNTKNASIE 66

Query: 66  DNWFDKIAIGHLSQAVQATSG--WRSKKSGYESLLEVTTMASRNFNQIKQREVVIQALGR 125
           D++F KIAI +LS+ +Q  +G    SK + Y+ L++  T  SRNF+  +Q E V+ +L R
Sbjct: 67  DSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFVLSSLDR 126

Query: 126 AFPKPILSLIKALLPQSKFAREYFAVFTTVFFAWLVGPCEVKESEFEGKIEKNVVQIHKC 178
           A P  I SLIK   P SK +RE FA+FTT+ FAWLVGP EV+E+E  G+ EK+VV I KC
Sbjct: 127 ALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKC 186

[Close]

BLAST of Lsi05G009680 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 109.0 bits (271), Expect = 5.7e-24
Identity = 62/171 (36.26%), Postives = 92/171 (53.80%), Query Frame = 0


Query: 79  GWRSKKSGYESLLE-VTTMASRNFNQIKQREVVIQALGRAFPKPILSLIKAL---LPQSK 138
           G  S+K GY  L+E V  +  +   + +  +  ++ L   FP  IL L K L   + Q K
Sbjct: 74  GSDSEKPGYVGLIELVKLLLLKGRTRSETSDAAVRILKSLFPPLILELYKLLIAPIAQGK 133

Query: 139 FAREYFAVFTTVFFAWLVGPCEVKESEF-EGKIEKNVVQIHKCRFLEQTNCAGMCINLCK 198
            A    A  T +   WL+GP +V   +   G+   + V + KC++LE++ C G+CIN CK
Sbjct: 134 LAALMVARVTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQYLEESKCVGVCINTCK 193

Query: 199 FPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAPIDDPALKQPCYKLC 245
            P Q F KD +G+P+ M PNF D SC+  FG  PP   DD  + +PC++ C
Sbjct: 194 LPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAPPE--DDGNVNEPCFETC 242

The following BLAST results are available for this feature:

BLAST of Lsi05G009680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
R4HZ96	3.7e-84	60.78	Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27...	[more]
C7AU21	7.9e-71	63.05	Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]
Q7XA78	8.5e-57	44.88	Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D2...	[more]

BLAST of Lsi05G009680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E3G1	9.0e-126	89.84	Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BL84	2.6e-125	89.45	LOW QUALITY PROTEIN: beta-carotene isomerase D27, chloroplastic OS=Cucumis melo ...	[more]
A0A0A0LIR1	3.4e-125	89.06	DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G226860 PE=...	[more]
A0A6J1HUP9	3.9e-121	84.88	beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita maxima O...	[more]
A0A6J1HHC5	9.6e-120	84.11	beta-carotene isomerase D27, chloroplastic-like isoform X3 OS=Cucurbita moschata...	[more]

BLAST of Lsi05G009680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888067.1	9.8e-135	95.31	beta-carotene isomerase D27, chloroplastic isoform X2 [Benincasa hispida]	[more]
XP_038888066.1	3.4e-127	95.49	beta-carotene isomerase D27, chloroplastic isoform X1 [Benincasa hispida]	[more]
KAA0057411.1	1.9e-125	89.84	beta-carotene isomerase D27 [Cucumis melo var. makuwa] >TYK30110.1 beta-carotene...	[more]
XP_008449360.1	5.4e-125	89.45	PREDICTED: LOW QUALITY PROTEIN: beta-carotene isomerase D27, chloroplastic [Cucu...	[more]
XP_004145371.1	7.0e-125	89.06	beta-carotene isomerase D27, chloroplastic [Cucumis sativus] >KGN61698.1 hypothe...	[more]

BLAST of Lsi05G009680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03055.1	6.1e-58	44.88	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G64680.1	2.9e-36	37.19	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G03055.2	4.2e-27	39.78	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT4G01995.1	5.7e-24	36.26	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025114	Beta-carotene isomerase D27-like, C-terminal	PFAM	PF13225	DUF4033	coord: 150..229 e-value: 3.4E-33 score: 113.5
None	No IPR available	PANTHER	PTHR33591:SF1	BETA-CAROTENE ISOMERASE D27, CHLOROPLASTIC	coord: 4..252
IPR038938	Beta-carotene isomerase D27-like	PANTHER	PTHR33591	BETA-CAROTENE ISOMERASE D27	coord: 4..252

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G009680.1	Lsi05G009680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010223	secondary shoot formation
biological_process	GO:1901601	strigolactone biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016859	cis-trans isomerase activity
molecular_function	GO:0005506	iron ion binding