Lsi05G009480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G009480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	CST complex subunit STN1
Location	chr05: 16607026 .. 16609434 (-)
RNA-Seq Expression	Lsi05G009480
Synteny	Lsi05G009480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATGAAGTTTAATTGGGCTCTCATGGATCTAAAAAAAGCCCAATAGAAGCCCAATGGGCCAGAAATTTAACTCAGTCAGAACCAGAGAATTACACATTAGGTTTCCGATGAACCAACGGCAACGCCTCTCCCTCAACCACACCCACATCAAGCTTCTTGGTTTCGACCTTAATTCCCTCACCCAAAATTCCTCTTTTTCTTCTTCTTCTGATGCAGTTTCTTTCTCTCGGAAAGGCTATGCCGTTTCCTGTACAGAGATCGTCGGCGTTGTGGTCTCTCGTGACCGCAAACCTAACAAGTTTCTCAAATTTTCCGTCGACGATGGTACCGCCTGTGTCGGTTGCATCCTGTGGCTAAATCATCTCACATCGCCTTATTTTGCTTCCTGCCATCAGGCAGATGTTCGAATAATGGGAAATATGGCGACCCATTTCGCATCCCAAATTAGGGTTGGGATTGTTGCTAGGGTTCGGGGGAGATTGAGCAGCTATAGGGGAGTGGTGCAGATTACAGTGTCAGATGTTGTGGTTGAGGACGACCCAAATGCTGAAATTCTGCATTGGTTGGATTCTATGAGGTTGGCTCTAAAGTGTTATGACCTCTCCCCTACACCCACTTAGCTGTAGATTCCAATGGTTTAAAATGCTATCTCTAGTCTTGTGATCTCCCCTTTTTTTTTTTTCCTTTCTGAATGCAGAAAAGAATCCGCTTTTTGTTGTTTTCTTTTTCCTTCTTTAAAGTTAAAAGTTGAAGTTTACTGTACAAAATTTTCCACAACAGCTTGGCCTTTGAAATGATTATATAATAATATTGAAACTAAGTTGCTAGTATGCTACTACTGTCTGTGTTTACTGTTTAGTGTGATGAAACTTGCTAATGTTCAATGAGGAACTTAATGACAAATAACCAATTGACAATTTTTTCTCGATTGGATATGATTAAATGGATGGTGGGATTGTGGGTACTAAGTACTAACATTTTTATTTATAAATTAAACCATTTAAAGCTGCAAATATTAATAAATATATGTACATATCAACCCATACCAGCTGCGACAAATTTTACCCATCCATTACATCAAGTAGCAGTGTAGATGTATGGGCCTTTACCTAAAGTTGTAGGTTTGTTTTTTCTAGTTTTTTTCTTTGGGGGGGTGTTCTGTGGGAGTTGATTCTTTTTGAATGAAAGATCATTTTGAGAGAAAGATATGGGTATTGGGGATTTTCTAGTTCCTAGCAGGATGGGCACAGGATGAACAATGGAAAGTCAGAATTATAAACAAAAGAATGGCTTTATGAACACGGATGAAAGGTAAGTTCAAACAGCAAAGTTGAATCCTCTGGTTACTGCATACCCCAAAATCACTCCACTTTTGACCTCAGTCAATTCAAAATTCTGTCACTGAGATTTCTCTATAGCTTCCAGTCATTCTTTCTTGCTCTGTTGCAACCTATGCATGAATATGCTTGGCTTTCTTTGATGCTCTCTCATTTCTAAGCTATTCATTCAACTAAATCTCCTGAACAAAGAGCATTTTCTTATTGGTGGTGTGAAAGAATACTGTTTGATATGTTTTGTCCATAAAGCACAAAATAGTGGTAGTGTTTCCATATATGCAATTGCTATATCTACTTTTTCTTTTTCCTCTTCTTGAACTGATTCCCCTGTGCTTCTAGAATAGGGATGTCAAACGAATCCAACCACTCTCAAAACTTGATCAGTTTTCCTCGAACTATTTTGGTTTGGTTGATTCAAGTTTGAATGAGTTGAATTCATATGGATGAACTTATTGATTAAGATTTATGTTAGTATCAACTGAGCTTAGATATATAATTATATTATCTTGGTGAAGCAAATTAGATAGACTCGAAAGATAACCCAAGTCTAATTTGCAGTTTTAGGTATTGAATTATATGCATTGAACCTCATGTTTTCATTCCAAATTGTTGTCATAATAAGGAGTAGAATAAAATAATGAAATAAAGCAGTTGCAATATTTTTGCTTTGAATTTAAATGGCCTTCAGGTTCAGGCTATAGCTCAGGTGGCTATAAAACACAGATCAGGTCGAGCTGGCTCTAACCTTCTTGATGGCTACGATGAAAAACAAAGAAGCCGGTGGACAAATTCCTCATGATTTGATGGGCTACAATGGAGAAAAACAAGGGCTATCAATTTATCTTCGTTTTTAATTAGGCCTATTCCTATTAGAAAATTAAATAAATAAAACAAATGCCTACTCTCAGTATTTCCCCCCCTAATTTTAATTAAATAACACTTGATTTCATGTGTGTAACACCGAGATCGTTGAAGTAGAATTTTTATTGTCAATTCTAGAATATTTTGGTGGACAAGACATTAACTAACATTTGATAATATTATATATAAATATAATCGATAGTTTGATTTT

mRNA sequence

CATGAAGTTTAATTGGGCTCTCATGGATCTAAAAAAAGCCCAATAGAAGCCCAATGGGCCAGAAATTTAACTCAGTCAGAACCAGAGAATTACACATTAGGTTTCCGATGAACCAACGGCAACGCCTCTCCCTCAACCACACCCACATCAAGCTTCTTGGTTTCGACCTTAATTCCCTCACCCAAAATTCCTCTTTTTCTTCTTCTTCTGATGCAGTTTCTTTCTCTCGGAAAGGCTATGCCGTTTCCTGTACAGAGATCGTCGGCGTTGTGGTCTCTCGTGACCGCAAACCTAACAAGTTTCTCAAATTTTCCGTCGACGATGGTACCGCCTGTGTCGGTTGCATCCTGTGGCTAAATCATCTCACATCGCCTTATTTTGCTTCCTGCCATCAGGCAGATGTTCGAATAATGGGAAATATGGCGACCCATTTCGCATCCCAAATTAGGGTTGGGATTGTTGCTAGGGTTCGGGGGAGATTGAGCAGCTATAGGGGAGTGGTGCAGATTACAGTGTCAGATGTTGTGGTTGAGGACGACCCAAATGCTGAAATTCTGCATTGGTTGGATTCTATGAGAATAGGGATGTCAAACGAATCCAACCACTCTCAAAACTTGATCAGTTTTCCTCGAACTATTTTGGTTCAGGCTATAGCTCAGGTGGCTATAAAACACAGATCAGGTCGAGCTGGCTCTAACCTTCTTGATGGCTACGATGAAAAACAAAGAAGCCGGTGGACAAATTCCTCATGATTTGATGGGCTACAATGGAGAAAAACAAGGGCTATCAATTTATCTTCGTTTTTAATTAGGCCTATTCCTATTAGAAAATTAAATAAATAAAACAAATGCCTACTCTCAGTATTTCCCCCCCTAATTTTAATTAAATAACACTTGATTTCATGTGTGTAACACCGAGATCGTTGAAGTAGAATTTTTATTGTCAATTCTAGAATATTTTGGTGGACAAGACATTAACTAACATTTGATAATATTATATATAAATATAATCGATAGTTTGATTTT

Coding sequence (CDS)

ATGGGCCAGAAATTTAACTCAGTCAGAACCAGAGAATTACACATTAGGTTTCCGATGAACCAACGGCAACGCCTCTCCCTCAACCACACCCACATCAAGCTTCTTGGTTTCGACCTTAATTCCCTCACCCAAAATTCCTCTTTTTCTTCTTCTTCTGATGCAGTTTCTTTCTCTCGGAAAGGCTATGCCGTTTCCTGTACAGAGATCGTCGGCGTTGTGGTCTCTCGTGACCGCAAACCTAACAAGTTTCTCAAATTTTCCGTCGACGATGGTACCGCCTGTGTCGGTTGCATCCTGTGGCTAAATCATCTCACATCGCCTTATTTTGCTTCCTGCCATCAGGCAGATGTTCGAATAATGGGAAATATGGCGACCCATTTCGCATCCCAAATTAGGGTTGGGATTGTTGCTAGGGTTCGGGGGAGATTGAGCAGCTATAGGGGAGTGGTGCAGATTACAGTGTCAGATGTTGTGGTTGAGGACGACCCAAATGCTGAAATTCTGCATTGGTTGGATTCTATGAGAATAGGGATGTCAAACGAATCCAACCACTCTCAAAACTTGATCAGTTTTCCTCGAACTATTTTGGTTCAGGCTATAGCTCAGGTGGCTATAAAACACAGATCAGGTCGAGCTGGCTCTAACCTTCTTGATGGCTACGATGAAAAACAAAGAAGCCGGTGGACAAATTCCTCATGA

Protein sequence

MGQKFNSVRTRELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGMSNESNHSQNLISFPRTILVQAIAQVAIKHRSGRAGSNLLDGYDEKQRSRWTNSS

Homology

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BLAST of Lsi05G009480 vs. ExPASy Swiss-Prot
Match: Q9LMK5 (CST complex subunit STN1 OS=Arabidopsis thaliana OX=3702 GN=STN1 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.4e-36
Identity = 81/154 (52.60%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 26  SLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVGVVVSRDRKPNKFLK 85
           SL  TH KL+  D+  LTQ+ + S+S   +     G  VS  EIVG +VSRD  P KFLK
Sbjct: 4   SLQSTHAKLVARDIQRLTQSPTESNSFSLLG----GACVSRVEIVGTIVSRDLTP-KFLK 63

Query: 86  FSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQIRVGIVARVRGRLSS 145
           F VDDGT CV C++WLN LTS YF+    A + ++ + A   A+QIR+G VARVRGR+ S
Sbjct: 64  FGVDDGTGCVTCVMWLNQLTSSYFSRWDPATILLLASAARKQAAQIRIGAVARVRGRVGS 123

Query: 146 YRGVVQITVSDVVVEDDPNAEILHWLDSMRIGMS 180
           YRGV+QIT +  V E DPNAEILHWL+ +++G S
Sbjct: 124 YRGVMQITANVAVAERDPNAEILHWLECLKLGQS 152

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BLAST of Lsi05G009480 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 4.4e-04
Identity = 32/141 (22.70%), Postives = 58/141 (41.13%), Query Frame = 0


Query: 48  FSSSSDAVSFSRKGYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSP 107
           F SS +       G +++   +VG+V  +D      ++F++DDGT  + C  W++     
Sbjct: 54  FQSSGEKSGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVS----- 113

Query: 108 YFASCHQADVRIMGNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEI 167
                   D R M          +R G   R+ G L +++G  Q+ V  V    D N   
Sbjct: 114 -----ETFDAREM--------ESVRDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVT 173

Query: 168 LHWLDSMRIGMSNESNHSQNL 189
            H+++ +     N  +  Q +
Sbjct: 174 FHYIECIHFYSQNSESQRQQV 176

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BLAST of Lsi05G009480 vs. ExPASy TrEMBL
Match: A0A6J1HUX0 (CST complex subunit STN1 OS=Cucurbita maxima OX=3661 GN=LOC111466921 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 6.1e-73
Identity = 145/177 (81.92%), Postives = 157/177 (88.70%), Query Frame = 0


Query: 1   MGQKFNSVRTRELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRK 60
           MGQ  NS RT E   RFPMNQ++  SL HTHIKLLGFDLNSLTQ+SS SSSSDAVSFSR+
Sbjct: 1   MGQMINSARTIEPQSRFPMNQQRAFSLYHTHIKLLGFDLNSLTQSSS-SSSSDAVSFSRR 60

Query: 61  GYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIM 120
           G+AVSC EIVGVVVSRD KPN+FL+FSVDDGT CV CILWLNHLTSPYFAS HQ DVRI+
Sbjct: 61  GFAVSCAEIVGVVVSRDLKPNRFLRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRII 120

Query: 121 GNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIG 178
           GNMATHFA+QIRVGIVARVRG++SSYRGVVQITVSDVVVE+DPNAEILHWLD M  G
Sbjct: 121 GNMATHFAAQIRVGIVARVRGKVSSYRGVVQITVSDVVVENDPNAEILHWLDCMSEG 176

[Close]

BLAST of Lsi05G009480 vs. ExPASy TrEMBL
Match: A0A6J1HFA9 (LOW QUALITY PROTEIN: CST complex subunit STN1 OS=Cucurbita moschata OX=3662 GN=LOC111463780 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.2e-70
Identity = 138/167 (82.63%), Postives = 152/167 (91.02%), Query Frame = 0


Query: 12  ELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVG 71
           EL  RFPMNQ++  SL HTHIKLLGFDLNSLTQ++S SSSSDAVSFSR+GYAVSC EIVG
Sbjct: 34  ELRSRFPMNQQRAFSLYHTHIKLLGFDLNSLTQSAS-SSSSDAVSFSRRGYAVSCVEIVG 93

Query: 72  VVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQI 131
           VVV RD KPN+FL+FSVDDGT CV CILWLNHLTSPYFAS HQ DVRI+GNMATHFA+QI
Sbjct: 94  VVVFRDLKPNRFLRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQI 153

Query: 132 RVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGM 179
           RVGIVARVRGR+SSYRGVVQ+TVSDVVVE+DPNAEILHWLD MR+ +
Sbjct: 154 RVGIVARVRGRVSSYRGVVQMTVSDVVVENDPNAEILHWLDCMRLAL 199

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BLAST of Lsi05G009480 vs. ExPASy TrEMBL
Match: A0A1S3BLT5 (CST complex subunit STN1 OS=Cucumis melo OX=3656 GN=LOC103491245 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 6.3e-70
Identity = 140/169 (82.84%), Postives = 150/169 (88.76%), Query Frame = 0


Query: 12  ELHIRFPMNQRQR--LSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEI 71
           +LHIRFPMNQRQR   SL  TH+KLLGFDLNSL QN    SSSD+VSFSRKGYAVSCTEI
Sbjct: 44  KLHIRFPMNQRQRFSFSLYQTHVKLLGFDLNSLNQN----SSSDSVSFSRKGYAVSCTEI 103

Query: 72  VGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFAS 131
           VGVVV RD KPN+FLKFSVDDGTACVGCILWLNHLTS YFAS H  DVRI+ +MATHFA+
Sbjct: 104 VGVVVFRDLKPNRFLKFSVDDGTACVGCILWLNHLTSSYFASRHHPDVRILADMATHFAA 163

Query: 132 QIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGM 179
           QIRVGIV RVRG+LSSYRG+VQITVSDVVVEDDPNAEILHWLDSMR+ M
Sbjct: 164 QIRVGIVVRVRGKLSSYRGMVQITVSDVVVEDDPNAEILHWLDSMRLAM 208

[Close]

BLAST of Lsi05G009480 vs. ExPASy TrEMBL
Match: A0A5D3E3H2 (CST complex subunit STN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001150 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 7.2e-66
Identity = 134/162 (82.72%), Postives = 143/162 (88.27%), Query Frame = 0


Query: 19  MNQRQR--LSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVGVVVSR 78
           MNQRQR   SL  TH+KLLGFDLNSL QN    SSSD+VSFSRKGYAVSCTEIVGVVV R
Sbjct: 1   MNQRQRFSFSLYQTHVKLLGFDLNSLNQN----SSSDSVSFSRKGYAVSCTEIVGVVVFR 60

Query: 79  DRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQIRVGIV 138
           D KPN+FLKFSVDDGTACVGCILWLNHLTS YFAS H  DVRI+ +MATHFA+QIRVGIV
Sbjct: 61  DLKPNRFLKFSVDDGTACVGCILWLNHLTSSYFASRHHPDVRILADMATHFAAQIRVGIV 120

Query: 139 ARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGM 179
            RVRG+LSSYRG+VQITVSDVVVEDDPNAEILHWLDSMR+ M
Sbjct: 121 VRVRGKLSSYRGMVQITVSDVVVEDDPNAEILHWLDSMRLAM 158

[Close]

BLAST of Lsi05G009480 vs. ExPASy TrEMBL
Match: A0A0A0LNV0 (OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G223720 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 8.0e-65
Identity = 132/162 (81.48%), Postives = 143/162 (88.27%), Query Frame = 0


Query: 19  MNQRQR--LSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVGVVVSR 78
           MNQR+R   SL  TH+KLL FDLNSL Q    +SSSD+VSFSRKGYAVSCTEIVGVVV R
Sbjct: 1   MNQRRRFSFSLYQTHVKLLAFDLNSLNQ----TSSSDSVSFSRKGYAVSCTEIVGVVVFR 60

Query: 79  DRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQIRVGIV 138
           D KPN+FLKFSVDDGTACVGCILWLNHLTS YFAS H  DVRI+G+MATHFA+QIRVGIV
Sbjct: 61  DLKPNRFLKFSVDDGTACVGCILWLNHLTSSYFASRHHQDVRILGDMATHFAAQIRVGIV 120

Query: 139 ARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGM 179
            RVRG+LSSYRG+VQITVSDVVVEDDPNAEILHWLDSMR+ M
Sbjct: 121 VRVRGKLSSYRGMVQITVSDVVVEDDPNAEILHWLDSMRLAM 158

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BLAST of Lsi05G009480 vs. NCBI nr
Match: XP_038887265.1 (CST complex subunit STN1 isoform X1 [Benincasa hispida])

HSP 1 Score: 314.3 bits (804), Expect = 8.7e-82
Identity = 160/197 (81.22%), Postives = 171/197 (86.80%), Query Frame = 0


Query: 1   MGQKFNSVRTRELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRK 60
           MGQKFNS R R+LHIRFPMNQRQRLSL HTHIKLLGFD+NSL Q SS SSSSDAVSFSR 
Sbjct: 1   MGQKFNSARIRKLHIRFPMNQRQRLSLYHTHIKLLGFDINSLNQKSSSSSSSDAVSFSRN 60

Query: 61  GYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIM 120
           GYAVSC+EIVGVVVS D KPN+FLKFSVDDGTACVGCILWLNHLTSPYFAS HQ DVRIM
Sbjct: 61  GYAVSCSEIVGVVVSHDLKPNRFLKFSVDDGTACVGCILWLNHLTSPYFASRHQPDVRIM 120

Query: 121 GNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGMSN 180
           GN+ATHFA+QIRVGIVARVRG+LSSYRGVVQITVSDVVVE+DPNAEILHWLDSMR  ++ 
Sbjct: 121 GNVATHFAAQIRVGIVARVRGKLSSYRGVVQITVSDVVVEEDPNAEILHWLDSMRFRLAQ 180

Query: 181 ESNHSQNLISFPRTILV 198
               +Q       T LV
Sbjct: 181 VGYKTQRSSWIDLTFLV 197

[Close]

BLAST of Lsi05G009480 vs. NCBI nr
Match: XP_038887266.1 (CST complex subunit STN1 isoform X2 [Benincasa hispida])

HSP 1 Score: 310.1 bits (793), Expect = 1.6e-80
Identity = 155/174 (89.08%), Postives = 163/174 (93.68%), Query Frame = 0


Query: 1   MGQKFNSVRTRELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRK 60
           MGQKFNS R R+LHIRFPMNQRQRLSL HTHIKLLGFD+NSL Q SS SSSSDAVSFSR 
Sbjct: 1   MGQKFNSARIRKLHIRFPMNQRQRLSLYHTHIKLLGFDINSLNQKSSSSSSSDAVSFSRN 60

Query: 61  GYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIM 120
           GYAVSC+EIVGVVVS D KPN+FLKFSVDDGTACVGCILWLNHLTSPYFAS HQ DVRIM
Sbjct: 61  GYAVSCSEIVGVVVSHDLKPNRFLKFSVDDGTACVGCILWLNHLTSPYFASRHQPDVRIM 120

Query: 121 GNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSM 175
           GN+ATHFA+QIRVGIVARVRG+LSSYRGVVQITVSDVVVE+DPNAEILHWLDSM
Sbjct: 121 GNVATHFAAQIRVGIVARVRGKLSSYRGVVQITVSDVVVEEDPNAEILHWLDSM 174

[Close]

BLAST of Lsi05G009480 vs. NCBI nr
Match: KAG6571457.1 (CST complex subunit STN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 288.5 bits (737), Expect = 5.1e-74
Identity = 145/179 (81.01%), Postives = 160/179 (89.39%), Query Frame = 0


Query: 1   MGQKFNSVRT-RELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSR 60
           MG++FNS RT  EL  RFPMNQ++  SL HTHIKLLGFDLNSLTQ+SS SSSSDAVSFSR
Sbjct: 1   MGRRFNSARTIEELRSRFPMNQQRAFSLYHTHIKLLGFDLNSLTQSSSSSSSSDAVSFSR 60

Query: 61  KGYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRI 120
           +GYAVSC EIVGVVV RD KPN+FL+FSVDDGT CV CILWLNHLTSPYFA  HQ DVRI
Sbjct: 61  RGYAVSCVEIVGVVVFRDLKPNRFLRFSVDDGTGCVSCILWLNHLTSPYFALRHQPDVRI 120

Query: 121 MGNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGM 179
           +GNMATHFA+QIRVGIVARVRG++SSYRGVVQITVSDVVVE+DPNAEILHWLD MR+ +
Sbjct: 121 IGNMATHFAAQIRVGIVARVRGKVSSYRGVVQITVSDVVVENDPNAEILHWLDCMRLAL 179

[Close]

BLAST of Lsi05G009480 vs. NCBI nr
Match: XP_022967373.1 (CST complex subunit STN1 [Cucurbita maxima] >XP_022967374.1 CST complex subunit STN1 [Cucurbita maxima] >XP_022967375.1 CST complex subunit STN1 [Cucurbita maxima])

HSP 1 Score: 283.9 bits (725), Expect = 1.3e-72
Identity = 145/177 (81.92%), Postives = 157/177 (88.70%), Query Frame = 0


Query: 1   MGQKFNSVRTRELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRK 60
           MGQ  NS RT E   RFPMNQ++  SL HTHIKLLGFDLNSLTQ+SS SSSSDAVSFSR+
Sbjct: 1   MGQMINSARTIEPQSRFPMNQQRAFSLYHTHIKLLGFDLNSLTQSSS-SSSSDAVSFSRR 60

Query: 61  GYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIM 120
           G+AVSC EIVGVVVSRD KPN+FL+FSVDDGT CV CILWLNHLTSPYFAS HQ DVRI+
Sbjct: 61  GFAVSCAEIVGVVVSRDLKPNRFLRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRII 120

Query: 121 GNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIG 178
           GNMATHFA+QIRVGIVARVRG++SSYRGVVQITVSDVVVE+DPNAEILHWLD M  G
Sbjct: 121 GNMATHFAAQIRVGIVARVRGKVSSYRGVVQITVSDVVVENDPNAEILHWLDCMSEG 176

[Close]

BLAST of Lsi05G009480 vs. NCBI nr
Match: XP_022963452.1 (LOW QUALITY PROTEIN: CST complex subunit STN1 [Cucurbita moschata])

HSP 1 Score: 275.4 bits (703), Expect = 4.5e-70
Identity = 138/167 (82.63%), Postives = 152/167 (91.02%), Query Frame = 0


Query: 12  ELHIRFPMNQRQRLSLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVG 71
           EL  RFPMNQ++  SL HTHIKLLGFDLNSLTQ++S SSSSDAVSFSR+GYAVSC EIVG
Sbjct: 34  ELRSRFPMNQQRAFSLYHTHIKLLGFDLNSLTQSAS-SSSSDAVSFSRRGYAVSCVEIVG 93

Query: 72  VVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQI 131
           VVV RD KPN+FL+FSVDDGT CV CILWLNHLTSPYFAS HQ DVRI+GNMATHFA+QI
Sbjct: 94  VVVFRDLKPNRFLRFSVDDGTGCVSCILWLNHLTSPYFASRHQPDVRIIGNMATHFAAQI 153

Query: 132 RVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEILHWLDSMRIGM 179
           RVGIVARVRGR+SSYRGVVQ+TVSDVVVE+DPNAEILHWLD MR+ +
Sbjct: 154 RVGIVARVRGRVSSYRGVVQMTVSDVVVENDPNAEILHWLDCMRLAL 199

[Close]

BLAST of Lsi05G009480 vs. TAIR 10
Match: AT1G07130.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 152.9 bits (385), Expect = 3.1e-37
Identity = 81/154 (52.60%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 26  SLNHTHIKLLGFDLNSLTQNSSFSSSSDAVSFSRKGYAVSCTEIVGVVVSRDRKPNKFLK 85
           SL  TH KL+  D+  LTQ+ + S+S   +     G  VS  EIVG +VSRD  P KFLK
Sbjct: 4   SLQSTHAKLVARDIQRLTQSPTESNSFSLLG----GACVSRVEIVGTIVSRDLTP-KFLK 63

Query: 86  FSVDDGTACVGCILWLNHLTSPYFASCHQADVRIMGNMATHFASQIRVGIVARVRGRLSS 145
           F VDDGT CV C++WLN LTS YF+    A + ++ + A   A+QIR+G VARVRGR+ S
Sbjct: 64  FGVDDGTGCVTCVMWLNQLTSSYFSRWDPATILLLASAARKQAAQIRIGAVARVRGRVGS 123

Query: 146 YRGVVQITVSDVVVEDDPNAEILHWLDSMRIGMS 180
           YRGV+QIT +  V E DPNAEILHWL+ +++G S
Sbjct: 124 YRGVMQITANVAVAERDPNAEILHWLECLKLGQS 152

[Close]

BLAST of Lsi05G009480 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 46.6 bits (109), Expect = 3.2e-05
Identity = 32/141 (22.70%), Postives = 58/141 (41.13%), Query Frame = 0


Query: 48  FSSSSDAVSFSRKGYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSP 107
           F SS +       G +++   +VG+V  +D      ++F++DDGT  + C  W++     
Sbjct: 54  FQSSGEKSGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVS----- 113

Query: 108 YFASCHQADVRIMGNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEI 167
                   D R M          +R G   R+ G L +++G  Q+ V  V    D N   
Sbjct: 114 -----ETFDAREM--------ESVRDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVT 173

Query: 168 LHWLDSMRIGMSNESNHSQNL 189
            H+++ +     N  +  Q +
Sbjct: 174 FHYIECIHFYSQNSESQRQQV 176

[Close]

BLAST of Lsi05G009480 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 46.6 bits (109), Expect = 3.2e-05
Identity = 32/141 (22.70%), Postives = 58/141 (41.13%), Query Frame = 0


Query: 48  FSSSSDAVSFSRKGYAVSCTEIVGVVVSRDRKPNKFLKFSVDDGTACVGCILWLNHLTSP 107
           F SS +       G +++   +VG+V  +D      ++F++DDGT  + C  W++     
Sbjct: 54  FQSSGEKSGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVS----- 113

Query: 108 YFASCHQADVRIMGNMATHFASQIRVGIVARVRGRLSSYRGVVQITVSDVVVEDDPNAEI 167
                   D R M          +R G   R+ G L +++G  Q+ V  V    D N   
Sbjct: 114 -----ETFDAREM--------ESVRDGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVT 173

Query: 168 LHWLDSMRIGMSNESNHSQNL 189
            H+++ +     N  +  Q +
Sbjct: 174 FHYIECIHFYSQNSESQRQQV 176

The following BLAST results are available for this feature:

BLAST of Lsi05G009480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMK5	4.4e-36	52.60	CST complex subunit STN1 OS=Arabidopsis thaliana OX=3702 GN=STN1 PE=1 SV=1	[more]
Q9ZQ19	4.4e-04	22.70	Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ...	[more]

BLAST of Lsi05G009480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HUX0	6.1e-73	81.92	CST complex subunit STN1 OS=Cucurbita maxima OX=3661 GN=LOC111466921 PE=4 SV=1	[more]
A0A6J1HFA9	2.2e-70	82.63	LOW QUALITY PROTEIN: CST complex subunit STN1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A1S3BLT5	6.3e-70	82.84	CST complex subunit STN1 OS=Cucumis melo OX=3656 GN=LOC103491245 PE=4 SV=1	[more]
A0A5D3E3H2	7.2e-66	82.72	CST complex subunit STN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0LNV0	8.0e-65	81.48	OB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G223720 PE=4 SV=...	[more]

BLAST of Lsi05G009480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887265.1	8.7e-82	81.22	CST complex subunit STN1 isoform X1 [Benincasa hispida]	[more]
XP_038887266.1	1.6e-80	89.08	CST complex subunit STN1 isoform X2 [Benincasa hispida]	[more]
KAG6571457.1	5.1e-74	81.01	CST complex subunit STN1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022967373.1	1.3e-72	81.92	CST complex subunit STN1 [Cucurbita maxima] >XP_022967374.1 CST complex subunit ...	[more]
XP_022963452.1	4.5e-70	82.63	LOW QUALITY PROTEIN: CST complex subunit STN1 [Cucurbita moschata]	[more]

BLAST of Lsi05G009480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07130.1	3.1e-37	52.60	Nucleic acid-binding, OB-fold-like protein	[more]
AT2G24490.1	3.2e-05	22.70	replicon protein A2	[more]
AT2G24490.2	3.2e-05	22.70	replicon protein A2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.50.140		coord: 25..189 e-value: 3.6E-23 score: 83.6
None	No IPR available	PANTHER	PTHR13989:SF33	CST COMPLEX SUBUNIT STN1	coord: 26..188
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 69..158 e-value: 7.7E-10 score: 38.6
IPR040260	Replication factor A protein-like	PANTHER	PTHR13989	REPLICATION PROTEIN A-RELATED	coord: 26..188
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 60..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G009480.1	Lsi05G009480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003676	nucleic acid binding