Lsi05G009240 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G009240
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Adenylate isopentenyltransferase
Location	chr05: 16405444 .. 16406397 (+)
RNA-Seq Expression	Lsi05G009240
Synteny	Lsi05G009240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAAGCAAGGGATGAGTAGTAATTTAGGAATGGTAAATCATAATTACCTTGAGGTGAAAGCGAAGGAAAAAGTGGTAGTTGTGATGGGGCCCACAGGGAGTGGAAAATCCAAGCTGTCTATTGATCTGGCCACCCATTTCCCGAATTCCGAAATAATAAACTCCGACAAAATCCAACTCTACCAAGGCCTCGACATTCTGACCAATAAAATCAGCCCTGAAGACCAACGTGGCATCCCCCACCACTTACTTGGCATAACCACCACCCCTCACCTCGACATCCCCGTCACCAAATTCTGCCACATGGCTTCCCTCGCCGCCGCCTCCATCGTCCGCCGCCACCACCTCCCCATCATAGTCGGCGGCTCCAACTCCTACATCGAAGCTCTACTAGACCACTACTCAATGTTTTATTCCAAATACGAATGTTGCTTGTTGTGGGTGGACGTCGCGATGCCGCTACTCCATTCCTTTGTGTCGGACCGAGTGGACCAGATGGTTGCAAATGGTCTCGTGGAGGAGGTTCAAGGCTTGTTCGATCCGAATAACGTGGATTATTCTTATGGCATTAGAAAGGCAATTGGCGTACGTGAGCTAGACCCTTATCTTCGTAATTATAATAATTTAGTTTTACGAAATAAGCTGTTGAAAGAAGGGATAGCGGAGATTAAAGCAAATAGCTGCGAATTGGCGTGTAGGCAATTGAAGAAAATTCTACGGCTAAGAAATGTGATAGGGTGGAACATACATAGAGTGGATGCGACGGAGGCGTTTAGAAAGAATAAAACAAAATTTGAAATGGAGGAGGCATGGGCTAAGCACGTGAGTGGACCGAGTATTATGATTGTGGACCAATTTTTGAGTACACCAACAACTCACCTTCAACATCAACCCATTATTCTCAAACCTAAGCTTTTCAGAATGCCGCTTGCTGCAGCTGCTGCCCCTTAG

mRNA sequence

ATGAGCAAGCAAGGGATGAGTAGTAATTTAGGAATGGTAAATCATAATTACCTTGAGGTGAAAGCGAAGGAAAAAGTGGTAGTTGTGATGGGGCCCACAGGGAGTGGAAAATCCAAGCTGTCTATTGATCTGGCCACCCATTTCCCGAATTCCGAAATAATAAACTCCGACAAAATCCAACTCTACCAAGGCCTCGACATTCTGACCAATAAAATCAGCCCTGAAGACCAACGTGGCATCCCCCACCACTTACTTGGCATAACCACCACCCCTCACCTCGACATCCCCGTCACCAAATTCTGCCACATGGCTTCCCTCGCCGCCGCCTCCATCGTCCGCCGCCACCACCTCCCCATCATAGTCGGCGGCTCCAACTCCTACATCGAAGCTCTACTAGACCACTACTCAATGTTTTATTCCAAATACGAATGTTGCTTGTTGTGGGTGGACGTCGCGATGCCGCTACTCCATTCCTTTGTGTCGGACCGAGTGGACCAGATGGTTGCAAATGGTCTCGTGGAGGAGGTTCAAGGCTTGTTCGATCCGAATAACGTGGATTATTCTTATGGCATTAGAAAGGCAATTGGCGTACGTGAGCTAGACCCTTATCTTCGTAATTATAATAATTTAGTTTTACGAAATAAGCTGTTGAAAGAAGGGATAGCGGAGATTAAAGCAAATAGCTGCGAATTGGCGTGTAGGCAATTGAAGAAAATTCTACGGCTAAGAAATGTGATAGGGTGGAACATACATAGAGTGGATGCGACGGAGGCGTTTAGAAAGAATAAAACAAAATTTGAAATGGAGGAGGCATGGGCTAAGCACGTGAGTGGACCGAGTATTATGATTGTGGACCAATTTTTGAGTACACCAACAACTCACCTTCAACATCAACCCATTATTCTCAAACCTAAGCTTTTCAGAATGCCGCTTGCTGCAGCTGCTGCCCCTTAG

Coding sequence (CDS)

ATGAGCAAGCAAGGGATGAGTAGTAATTTAGGAATGGTAAATCATAATTACCTTGAGGTGAAAGCGAAGGAAAAAGTGGTAGTTGTGATGGGGCCCACAGGGAGTGGAAAATCCAAGCTGTCTATTGATCTGGCCACCCATTTCCCGAATTCCGAAATAATAAACTCCGACAAAATCCAACTCTACCAAGGCCTCGACATTCTGACCAATAAAATCAGCCCTGAAGACCAACGTGGCATCCCCCACCACTTACTTGGCATAACCACCACCCCTCACCTCGACATCCCCGTCACCAAATTCTGCCACATGGCTTCCCTCGCCGCCGCCTCCATCGTCCGCCGCCACCACCTCCCCATCATAGTCGGCGGCTCCAACTCCTACATCGAAGCTCTACTAGACCACTACTCAATGTTTTATTCCAAATACGAATGTTGCTTGTTGTGGGTGGACGTCGCGATGCCGCTACTCCATTCCTTTGTGTCGGACCGAGTGGACCAGATGGTTGCAAATGGTCTCGTGGAGGAGGTTCAAGGCTTGTTCGATCCGAATAACGTGGATTATTCTTATGGCATTAGAAAGGCAATTGGCGTACGTGAGCTAGACCCTTATCTTCGTAATTATAATAATTTAGTTTTACGAAATAAGCTGTTGAAAGAAGGGATAGCGGAGATTAAAGCAAATAGCTGCGAATTGGCGTGTAGGCAATTGAAGAAAATTCTACGGCTAAGAAATGTGATAGGGTGGAACATACATAGAGTGGATGCGACGGAGGCGTTTAGAAAGAATAAAACAAAATTTGAAATGGAGGAGGCATGGGCTAAGCACGTGAGTGGACCGAGTATTATGATTGTGGACCAATTTTTGAGTACACCAACAACTCACCTTCAACATCAACCCATTATTCTCAAACCTAAGCTTTTCAGAATGCCGCTTGCTGCAGCTGCTGCCCCTTAG

Protein sequence

MSKQGMSSNLGMVNHNYLEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLSTPTTHLQHQPIILKPKLFRMPLAAAAAP

Homology

[Close]

BLAST of Lsi05G009240 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-78
Identity = 150/269 (55.76%), Postives = 199/269 (73.98%), Query Frame = 0


Query: 23  KEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPH 82
           K+KVVV+MG TG+GKS+LS+D+AT F  +EIINSDKIQ++QGLDI+TNKI+ E+  G+PH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRF-RAEIINSDKIQVHQGLDIVTNKITSEESCGVPH 99

Query: 83  HLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL-DHYSMFYSK 142
           HLLG+   P  D+    +CHMA+L+  S++ R  LPIIVGGSNSY+EAL+ D  + F S+
Sbjct: 100 HLLGV-LPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSR 159

Query: 143 YECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGIRKAIGVRELD 202
           Y+CC LWVDVA+P+LH FVS+RVD+MV +G+VEEV+  FD +N DYS GI+KAIG  E D
Sbjct: 160 YDCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFD 219

Query: 203 PYLRN--YNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWNIHRVDATEAF 262
            + RN  + N+  R +LL + + EIK N+ ELACRQ +KI RLR V  W+I RVDAT  F
Sbjct: 220 RFFRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 263 RKNKTKFEMEEAWAKHVSGPSIMIVDQFL 289
            K ++K +   AW + V+GPS   V +FL
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFL 306

[Close]

BLAST of Lsi05G009240 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 286.6 bits (732), Expect = 3.5e-76
Identity = 157/299 (52.51%), Postives = 203/299 (67.89%), Query Frame = 0


Query: 21  KAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGI 80
           + K+KVV VMG TG+GKS+L+IDLAT FP +EI+NSDKIQ+Y+GLDI+TNK++PE+  G+
Sbjct: 30  RRKDKVVFVMGATGTGKSRLAIDLATRFP-AEIVNSDKIQVYKGLDIVTNKVTPEESLGV 89

Query: 81  PHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALLDHYSMFYS 140
           PHHLLG     + D     F   A  A  SIV+R  +PII GGSNSYIEAL++    F  
Sbjct: 90  PHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRL 149

Query: 141 KYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGIRKAIGVREL 200
           +Y CC LWVDV+ P+LHSFVS+RVD+MV  GLV+EV+ +FDP++ DYS GIR+AIGV EL
Sbjct: 150 RYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPEL 209

Query: 201 DPYL----RNYNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWNIHRVDAT 260
           D +L    RNY       +LL+  I +IK N+C LACRQL+KI RL     WN+HRVDAT
Sbjct: 210 DEFLRSEMRNY-PAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDAT 269

Query: 261 EAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLSTPTTHLQHQPIILKPKLFRMPLAAAA 316
           E F +     E +EAW   V+ PS + V++FLS    H      IL P++  +P   AA
Sbjct: 270 EVFLRRGE--EADEAWDNSVAHPSALAVEKFLSYSDDHHLEGANILLPEISAVPPLPAA 324

[Close]

BLAST of Lsi05G009240 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 267.3 bits (682), Expect = 2.2e-70
Identity = 146/283 (51.59%), Postives = 199/283 (70.32%), Query Frame = 0


Query: 8   SNLGMVNHNYLEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDI 67
           + L  VN N   +  KEKV+ VMG TGSGKS+L+IDLAT F   EIINSDKIQLY+GLD+
Sbjct: 19  NKLSKVNVNSF-LHPKEKVIFVMGATGSGKSRLAIDLATRF-QGEIINSDKIQLYKGLDV 78

Query: 68  LTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSY 127
           LTNK++P++ RG+PHHLLG+  +   ++  T++  +AS A + +   + LPI+ GGSNSY
Sbjct: 79  LTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSY 138

Query: 128 IEALLDHYSMF-YSKYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVD 187
           IEAL++H S F  + Y+CC +WVDV++P+L+SFVS RVD+M+  GL+EEV+ +F+P   +
Sbjct: 139 IEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNP-KAN 198

Query: 188 YSYGIRKAIGVRELDPYLRNYN--NLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRN 247
           YS GIR+AIGV EL  YLRN +  +   ++K+L   +  IK N+  LACRQLKKI RL  
Sbjct: 199 YSVGIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHK 258

Query: 248 VIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQF 288
               ++HRVDATE F K   + E +EAW   V+ PS  IVD+F
Sbjct: 259 KWKMSMHRVDATEVFLKRNVE-EQDEAWENLVARPSERIVDKF 297

[Close]

BLAST of Lsi05G009240 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 125/293 (42.66%), Postives = 183/293 (62.46%), Query Frame = 0


Query: 23  KEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPH 82
           K+KVVV++G TG+GKS+LS+DLAT FP SEIINSDKIQ+Y+GL+I TN+I+ +D+RG+PH
Sbjct: 64  KDKVVVILGATGAGKSRLSVDLATRFP-SEIINSDKIQVYEGLEITTNQITLQDRRGVPH 123

Query: 83  HLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL-----DHYSM 142
           HLLG+    H ++   +F   AS     I  R  +PII GGSNS++ ALL       +  
Sbjct: 124 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 183

Query: 143 FYS---------KYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDP--NNVD 202
           F S         +YECC +WVDV+  +L+ ++  RVD+M+ +G+ EE+   +DP  + ++
Sbjct: 184 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 243

Query: 203 YSYGIRKAIGVRELDPYLRNY---NNLV----LRNKLLKEGIAEIKANSCELACRQLKKI 262
             +GIRKAIGV E D Y + Y     ++    LR     + + +IK N+  LA RQ+KKI
Sbjct: 244 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 303

Query: 263 LRLRNVIGWNIHRVDATEAFR----KNKTKFEMEEAWAKHVSGPSIMIVDQFL 289
             L++  GW I RVDAT +F+    K+ ++ +  E W + V  PS+ IV + L
Sbjct: 304 EMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

[Close]

BLAST of Lsi05G009240 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.6e-55
Identity = 127/301 (42.19%), Postives = 176/301 (58.47%), Query Frame = 0


Query: 23  KEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPH 82
           KEK++V+MG TG+GKS+LSIDLA HFP  E+INSDK+Q+Y+GLDI TNKIS  D+ G+PH
Sbjct: 29  KEKLLVLMGATGTGKSRLSIDLAAHFP-LEVINSDKMQVYKGLDITTNKISVPDRGGVPH 88

Query: 83  HLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL---------- 142
           HLLG       ++    F  +A  A + I  R  LP++VGGSNS+I ALL          
Sbjct: 89  HLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPG 148

Query: 143 ----DHYSMFYS--KYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVD 202
                 +S+  S  +Y+CC LWVDV++ +L  +++ RVD M+  G+ +E+   + P + D
Sbjct: 149 VFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDED 208

Query: 203 YS------YGIRKAIGVRELDPYLRNY----------NNLVLRNKLLKEGIAEIKANSCE 262
           +        G+RKAIGV E D Y   +              +R    +E +  IK N+C 
Sbjct: 209 HDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCH 268

Query: 263 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEME---EAWAKHVSGPSIMIVDQF 289
           LA RQ+ KILRL+   GW++ R+DATE+FR   T    E   E W K V  PS+ IV +F
Sbjct: 269 LAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRF 327

[Close]

BLAST of Lsi05G009240 vs. ExPASy TrEMBL
Match: A0A6J1HJ23 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111464017 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 1.0e-107
Identity = 219/324 (67.59%), Postives = 244/324 (75.31%), Query Frame = 0


Query: 6   MSSNLGMVNHNY--------------LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNS 65
           M SNL MVNH                  +K K+KVVVVMG TGSGKS+LSIDLATHF N 
Sbjct: 1   MISNLAMVNHELPFGEVNIKGLTPARRLIKEKQKVVVVMGATGSGKSRLSIDLATHFANC 60

Query: 66  EIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASI 125
           EIINSDKIQL+ GLDI+TNKI+P +QRG+PHHLLGI  +P LD  V KFC MA+LAA SI
Sbjct: 61  EIINSDKIQLHDGLDIVTNKITPNEQRGVPHHLLGI-ASPFLDFSVAKFCRMATLAADSI 120

Query: 126 VRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANG 185
           V R HLPIIVGGSNSY+EAL+D Y  F SKYECC LWVDV+MPLLHS VSDRVD+MV NG
Sbjct: 121 VHRTHLPIIVGGSNSYVEALVDDYPTFRSKYECCFLWVDVSMPLLHSSVSDRVDKMVGNG 180

Query: 186 LVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYN-NLVLRNKLLKEGIAEIKANSCE 245
           LVEEV+ LFDPNNVDYS+GIRKAIGV ELD YLR  N +  +R KLL+E IAEIKAN+CE
Sbjct: 181 LVEEVRNLFDPNNVDYSHGIRKAIGVPELDRYLRASNSDYEIREKLLQEAIAEIKANTCE 240

Query: 246 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLST 305
           LACRQ+ KILRLRNV GW IHRVDATEAFRK K K E +EAW K V+GPS +IV  FL+ 
Sbjct: 241 LACRQVDKILRLRNVKGWKIHRVDATEAFRK-KGK-EGDEAWTKLVAGPSALIVAHFLTQ 300

Query: 306 PTTHLQHQPIILKPKLFRMPLAAA 315
           P     H    LK K FRMP   A
Sbjct: 301 P-----HGQPYLKHKAFRMPFKRA 316

[Close]

BLAST of Lsi05G009240 vs. ExPASy TrEMBL
Match: A0A6J1HUH5 (adenylate isopentenyltransferase 3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466978 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 3.4e-106
Identity = 219/331 (66.16%), Postives = 247/331 (74.62%), Query Frame = 0


Query: 6   MSSNLGMVNHNY--------------LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNS 65
           M SNL MVNH                  +K K+KVVVVMG TGSGKS+LSIDLATHF N 
Sbjct: 1   MISNLAMVNHELPFGEVNIKGLTPARRLIKEKQKVVVVMGATGSGKSRLSIDLATHFANC 60

Query: 66  EIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASI 125
           EIINSDKIQL+ GLDI+TNKI+P++QRG+PHHLLGI  +P LD  V KFC MA+LAA SI
Sbjct: 61  EIINSDKIQLHDGLDIVTNKITPDEQRGVPHHLLGI-ASPFLDFSVAKFCRMATLAADSI 120

Query: 126 VRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANG 185
           V R +LPIIVGGSNSY+EAL+D YS F SKYECC LWVDV+MP+LHS VSDRVD+MV NG
Sbjct: 121 VHRANLPIIVGGSNSYVEALVDDYSTFRSKYECCFLWVDVSMPILHSSVSDRVDKMVGNG 180

Query: 186 LVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYN-NLVLRNKLLKEGIAEIKANSCE 245
           LVEEV+ L+DPNNVDYSYGIR+AIGV ELD Y R  N +   R KLL+E IAEIKAN+C+
Sbjct: 181 LVEEVRNLYDPNNVDYSYGIRRAIGVPELDHYFRAPNSDYETREKLLQEAIAEIKANTCD 240

Query: 246 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLST 305
           LACRQ+ KILRLRNV GW IHRVDATEAFRK K K E +EAW K V+GPS  IV  FL+ 
Sbjct: 241 LACRQVDKILRLRNVKGWKIHRVDATEAFRK-KGK-EADEAWTKLVAGPSATIVAHFLTQ 300

Query: 306 PTTHLQHQPIILKPKLFRMPL-----AAAAA 317
           P     H    LK K FRMP      AAAAA
Sbjct: 301 P-----HGQPYLKHKAFRMPFKRAPTAAAAA 323

[Close]

BLAST of Lsi05G009240 vs. ExPASy TrEMBL
Match: A0A6P9FA20 (adenylate isopentenyltransferase 3, chloroplastic OS=Juglans regia OX=51240 GN=LOC109010913 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 8.1e-84
Identity = 173/299 (57.86%), Postives = 218/299 (72.91%), Query Frame = 0


Query: 7   SSNLGMVNHNYLEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLD 66
           S  L M   N   ++ KEKVV+VMG TG+GKS+LSIDLAT FP +EIINSDK+Q+Y+GL 
Sbjct: 21  SGGLKMDVFNPHRIRHKEKVVIVMGATGTGKSRLSIDLATRFP-AEIINSDKMQVYEGLH 80

Query: 67  ILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNS 126
           I+TNK++ E+QRG+PHHLLGI   P  D  VT FC  AS+A  SI+ R  LPIIVGGSNS
Sbjct: 81  IVTNKVTEEEQRGVPHHLLGI-LNPSADFTVTNFCDKASIAIESILGRGRLPIIVGGSNS 140

Query: 127 YIEALLDHYS-MFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNV 186
           Y+EAL+++   M  SKY+CC LWVDV+MP+LHSFVS RVDQMV NG+V+E++ +FDP N 
Sbjct: 141 YMEALVENDDYMLRSKYDCCFLWVDVSMPVLHSFVSKRVDQMVENGMVDELRNMFDP-NA 200

Query: 187 DYSYGIRKAIGVRELDPYLRN--YNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLR 246
           D S GIR+AIGV E D Y RN  + +   R  LL+E I ++K N+C+LACRQL KI RLR
Sbjct: 201 DNSRGIRRAIGVPEFDQYFRNEPFLDRKQRGILLQEAINQMKENTCKLACRQLGKIHRLR 260

Query: 247 NVIGWNIHRVDATEAFRKNKTKFEMEEA-WAKHVSGPSIMIVDQFL------STPTTHL 296
           N+ GWN+HR+DATE F+K     E +EA W + V+GPS +IV QFL        PTTH+
Sbjct: 261 NIKGWNMHRIDATEVFQKKGRSSEADEAIWEQVVAGPSTVIVGQFLYNVAASMVPTTHV 316

[Close]

BLAST of Lsi05G009240 vs. ExPASy TrEMBL
Match: A0A5N6QRB0 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_006115 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 1.4e-83
Identity = 170/279 (60.93%), Postives = 212/279 (75.99%), Query Frame = 0


Query: 13  VNHNYLEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKI 72
           +N+ +   + +EKVV++MG TG+GKS+LSIDLAT F  +EIINSDK+Q Y+GL I TNK+
Sbjct: 25  MNNVFNPRRHREKVVILMGATGTGKSRLSIDLATRFA-AEIINSDKVQAYEGLPIATNKV 84

Query: 73  SPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL 132
           + E+QRG+PHHLLGI   P+ D   T FC  ASLA  SI+ R  LP+IVGGSNSYIEAL+
Sbjct: 85  TEEEQRGVPHHLLGI-LNPNADFTATDFCDRASLAVESILGRDRLPLIVGGSNSYIEALI 144

Query: 133 DHYS-MFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGI 192
           D    MF SKYECC LWVDV+MP+LHSFVS+RVDQMV NGLV+EV+ LFDP   DYS GI
Sbjct: 145 DDDDYMFRSKYECCFLWVDVSMPVLHSFVSNRVDQMVENGLVDEVRKLFDP-AADYSRGI 204

Query: 193 RKAIGVRELDPYLRN--YNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWN 252
           R+AIGV E D Y R   + +  LR +LL + I ++K N+C+LACRQL KILRLRN  GWN
Sbjct: 205 RRAIGVPEFDQYFRTEPFLDKHLRLRLLHDAITQMKENTCKLACRQLGKILRLRNKKGWN 264

Query: 253 IHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFL 289
           +HR+DATE F+K+ +K E +EAW + V+GPS  IV QFL
Sbjct: 265 MHRIDATEVFQKHGSK-EADEAWMELVAGPSKEIVGQFL 299

[Close]

BLAST of Lsi05G009240 vs. ExPASy TrEMBL
Match: A0A6A1UVG0 (Adenylate isopentenyltransferase 3, chloroplastic OS=Morella rubra OX=262757 GN=CJ030_MR8G027188 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.8e-83
Identity = 172/295 (58.31%), Postives = 213/295 (72.20%), Query Frame = 0


Query: 23  KEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPH 82
           KEKVV+VMG TG+GKS+LSIDLATHFP +EIINSDK+Q+Y+GL I+TNK++ E+QRG+PH
Sbjct: 10  KEKVVIVMGATGTGKSRLSIDLATHFP-AEIINSDKMQVYEGLPIVTNKVTDEEQRGVPH 69

Query: 83  HLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALLDHYSMFY-SK 142
           HLLGI   P  D  VT FC  ASLA  S++ R  LPIIVGGSNSYIEAL+D     + SK
Sbjct: 70  HLLGI-LNPSADFTVTNFCDKASLAIESVLGRGRLPIIVGGSNSYIEALVDGDDYLHRSK 129

Query: 143 YECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGIRKAIGVRELD 202
           YECC LWVDV+MPLLHSFVS RVDQM+ NG+V+E++ +FDP   DYS GIR+AIGV E D
Sbjct: 130 YECCFLWVDVSMPLLHSFVSKRVDQMLENGMVDELRKMFDPIAEDYSRGIRRAIGVPEFD 189

Query: 203 PYLRN--YNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWNIHRVDATEAF 262
            Y RN  + +   R KLL+E I ++K N+ +LACRQL KI RLRN+ GWN HR+DATE F
Sbjct: 190 QYFRNEPFLDQEHRAKLLQEAITQMKENTSKLACRQLGKIHRLRNIKGWNTHRLDATEVF 249

Query: 263 RKNKTKFEMEEAWAKHVSGPSIMIVDQFLSTPTTHLQHQPIILKPKLFRMPLAAA 315
           +K     E +EAW + V+GPS  IV QFL    T +     + K +     +A A
Sbjct: 250 QKQGK--EADEAWKRLVAGPSAHIVGQFLYNVPTLVPTNVAVSKVRKVATAIATA 300

[Close]

BLAST of Lsi05G009240 vs. NCBI nr
Match: XP_038887422.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Benincasa hispida] >XP_038887423.1 adenylate isopentenyltransferase 3, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 495.7 bits (1275), Expect = 2.9e-136
Identity = 257/331 (77.64%), Postives = 281/331 (84.89%), Query Frame = 0


Query: 1   MSKQGMSSNLGMVNHNYLE------------VKAKEKVVVVMGPTGSGKSKLSIDLATHF 60
           MSKQG+ SNLGM+N +Y++            VK KEKVVVVMG TGSGKSKLSIDLATHF
Sbjct: 11  MSKQGIRSNLGMINQDYVQVLNPRRVGDDNPVKGKEKVVVVMGATGSGKSKLSIDLATHF 70

Query: 61  PNSEIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAA 120
           PNSEIINSDK+QLYQGLD++TNKISP+DQRGIPHHLLGI TTPHLD+ V+KFCHMA+LAA
Sbjct: 71  PNSEIINSDKMQLYQGLDVVTNKISPQDQRGIPHHLLGIATTPHLDVTVSKFCHMATLAA 130

Query: 121 ASIVRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMV 180
            SIVRR  +PIIVGGSNSYIEALLD YS F+SKYECCLLWVDVAMPLLHSFVSDRVD+MV
Sbjct: 131 DSIVRRRRIPIIVGGSNSYIEALLDDYSTFHSKYECCLLWVDVAMPLLHSFVSDRVDKMV 190

Query: 181 ANGLVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRN-YNNLVLRNKLLKEGIAEIKAN 240
           A+GLVEEVQ LFDPNN DYS GIRKAIGV ELD Y R   N+ VLRNKLL+EGIAEIK N
Sbjct: 191 ASGLVEEVQELFDPNNGDYSRGIRKAIGVHELDRYFRACTNSKVLRNKLLQEGIAEIKEN 250

Query: 241 SCELACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQF 300
           SC+LACRQLKKILRL+NV GWNIHRVDATE FRK K+K E EEAWAKHV GPS+MIV  F
Sbjct: 251 SCKLACRQLKKILRLQNVKGWNIHRVDATEVFRKKKSKLEAEEAWAKHVIGPSVMIVAHF 310

Query: 301 LSTPTTHLQHQPIILKP-KLFRMPLAAAAAP 318
           LST  TH+Q +PIILKP  LFRM LAA  AP
Sbjct: 311 LST-KTHIQDRPIILKPTSLFRMSLAATVAP 340

[Close]

BLAST of Lsi05G009240 vs. NCBI nr
Match: KAG7011242.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 402.9 bits (1034), Expect = 2.5e-108
Identity = 224/332 (67.47%), Postives = 249/332 (75.00%), Query Frame = 0


Query: 6   MSSNLGMVNHNY--------------LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNS 65
           M SNL MVNH                  +K K+KVVVVMG TGSGKS+LSIDLATHF N 
Sbjct: 1   MISNLAMVNHELPFGEVNIKGLTPARRLIKEKQKVVVVMGATGSGKSRLSIDLATHFANC 60

Query: 66  EIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASI 125
           EIINSDKIQL+ GLDI+TNKI+P++QRG+PHHLLGI   P LD  V KFC MA+LAA SI
Sbjct: 61  EIINSDKIQLHDGLDIVTNKITPDEQRGVPHHLLGI-ANPFLDFSVAKFCSMATLAADSI 120

Query: 126 VRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANG 185
           V R HLPIIVGGSNSY+EAL+D YS F SKYECC LWVDV+MP+LHS VSDRVD+MV NG
Sbjct: 121 VHRAHLPIIVGGSNSYVEALVDDYSTFQSKYECCFLWVDVSMPILHSSVSDRVDKMVGNG 180

Query: 186 LVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYN-NLVLRNKLLKEGIAEIKANSCE 245
           LVEEV+ LFDPNNVDYS+GIRKAIGV ELD YLR  N +  +R KLL+E IAEIKAN+CE
Sbjct: 181 LVEEVRNLFDPNNVDYSHGIRKAIGVPELDRYLRASNSDYEIREKLLQEAIAEIKANTCE 240

Query: 246 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLST 305
           LACRQ+ KILRLRNV GW IHRVDATEAFRK K K E +EAW K V+GPS MIV  FL+ 
Sbjct: 241 LACRQVDKILRLRNVKGWKIHRVDATEAFRK-KGK-EGDEAWTKLVAGPSAMIVAHFLTQ 300

Query: 306 PTTHLQHQPIILKPKLFRMPL------AAAAA 317
           P     H    LK K FRMP       AAAAA
Sbjct: 301 P-----HGQPYLKHKAFRMPFKRAPTTAAAAA 324

[Close]

BLAST of Lsi05G009240 vs. NCBI nr
Match: KAG6571479.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 402.1 bits (1032), Expect = 4.3e-108
Identity = 223/332 (67.17%), Postives = 249/332 (75.00%), Query Frame = 0


Query: 6   MSSNLGMVNHNY--------------LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNS 65
           M SNL MVNH                  +K K+KVVVVMG TGSGKS+LSIDLATHF N 
Sbjct: 1   MISNLAMVNHELPFGEVNIKGLTPARRLIKEKQKVVVVMGATGSGKSRLSIDLATHFANC 60

Query: 66  EIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASI 125
           EIINSDKIQL+ GLDI+TNKI+P++QRG+PHHLLGI   P LD  V KFC MA+LAA SI
Sbjct: 61  EIINSDKIQLHDGLDIVTNKITPDEQRGVPHHLLGI-ANPFLDFNVAKFCSMATLAADSI 120

Query: 126 VRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANG 185
           + R HLPIIVGGSNSY+EAL+D YS F SKYECC LWVDV+MP+LHS VSDRVD+MV NG
Sbjct: 121 IHRAHLPIIVGGSNSYVEALVDDYSTFQSKYECCFLWVDVSMPILHSSVSDRVDKMVGNG 180

Query: 186 LVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYN-NLVLRNKLLKEGIAEIKANSCE 245
           LVEEV+ LFDPNNVDYS+GIRKAIGV ELD YLR  N +  +R KLL+E IAEIKAN+CE
Sbjct: 181 LVEEVRNLFDPNNVDYSHGIRKAIGVPELDRYLRASNSDYEIREKLLQEAIAEIKANTCE 240

Query: 246 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLST 305
           LACRQ+ KILRLRNV GW IHRVDATEAFRK K K E +EAW K V+GPS MIV  FL+ 
Sbjct: 241 LACRQVDKILRLRNVKGWKIHRVDATEAFRK-KGK-EGDEAWTKLVAGPSAMIVAHFLTQ 300

Query: 306 PTTHLQHQPIILKPKLFRMPL------AAAAA 317
           P     H    LK K FRMP       AAAAA
Sbjct: 301 P-----HGQPYLKHKAFRMPFKRAPTTAAAAA 324

[Close]

BLAST of Lsi05G009240 vs. NCBI nr
Match: XP_023553600.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.0 bits (1029), Expect = 9.7e-108
Identity = 223/332 (67.17%), Postives = 249/332 (75.00%), Query Frame = 0


Query: 6   MSSNLGMVNHNY--------------LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNS 65
           M SNL MVNH                  +K K+KVVVVMG TGSGKS+LSIDLATHF N 
Sbjct: 1   MISNLAMVNHELPFGEVNIKGLTPARRLIKEKQKVVVVMGATGSGKSRLSIDLATHFANC 60

Query: 66  EIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASI 125
           EIINSDKIQL+ GLDI+TNKI+P++QRG+PHHLLGI  +P LD  + KFC MA+LAA SI
Sbjct: 61  EIINSDKIQLHDGLDIVTNKITPDEQRGVPHHLLGI-ASPFLDFGIAKFCRMATLAADSI 120

Query: 126 VRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANG 185
           V R HLPIIVGGSNSY+EAL+D Y  F SKYECC LWVDV+MPLLHS VSDRVD+MV NG
Sbjct: 121 VHRAHLPIIVGGSNSYVEALVDDYPTFRSKYECCFLWVDVSMPLLHSSVSDRVDKMVENG 180

Query: 186 LVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYN-NLVLRNKLLKEGIAEIKANSCE 245
           LVEEV+ LFDPNNVDYS+GIRKAIGV ELD YLR  N +  +R KLL+E IAEIKAN+CE
Sbjct: 181 LVEEVRNLFDPNNVDYSHGIRKAIGVPELDRYLRASNSDYEIREKLLQEAIAEIKANTCE 240

Query: 246 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLST 305
           LACRQ+ KILRLRNV GW IHRVDATEAFRK K K E +EAW K V+GPS MIV  FL+ 
Sbjct: 241 LACRQVDKILRLRNVKGWKIHRVDATEAFRK-KGK-EGDEAWTKLVAGPSAMIVAHFLTQ 300

Query: 306 PTTHLQHQPIILKPKLFRMPL------AAAAA 317
           P     H    LK K FRMP       AAAAA
Sbjct: 301 P-----HGQPYLKHKAFRMPFKRAPTTAAAAA 324

[Close]

BLAST of Lsi05G009240 vs. NCBI nr
Match: XP_022963833.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 399.8 bits (1026), Expect = 2.2e-107
Identity = 219/324 (67.59%), Postives = 244/324 (75.31%), Query Frame = 0


Query: 6   MSSNLGMVNHNY--------------LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNS 65
           M SNL MVNH                  +K K+KVVVVMG TGSGKS+LSIDLATHF N 
Sbjct: 1   MISNLAMVNHELPFGEVNIKGLTPARRLIKEKQKVVVVMGATGSGKSRLSIDLATHFANC 60

Query: 66  EIINSDKIQLYQGLDILTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASI 125
           EIINSDKIQL+ GLDI+TNKI+P +QRG+PHHLLGI  +P LD  V KFC MA+LAA SI
Sbjct: 61  EIINSDKIQLHDGLDIVTNKITPNEQRGVPHHLLGI-ASPFLDFSVAKFCRMATLAADSI 120

Query: 126 VRRHHLPIIVGGSNSYIEALLDHYSMFYSKYECCLLWVDVAMPLLHSFVSDRVDQMVANG 185
           V R HLPIIVGGSNSY+EAL+D Y  F SKYECC LWVDV+MPLLHS VSDRVD+MV NG
Sbjct: 121 VHRTHLPIIVGGSNSYVEALVDDYPTFRSKYECCFLWVDVSMPLLHSSVSDRVDKMVGNG 180

Query: 186 LVEEVQGLFDPNNVDYSYGIRKAIGVRELDPYLRNYN-NLVLRNKLLKEGIAEIKANSCE 245
           LVEEV+ LFDPNNVDYS+GIRKAIGV ELD YLR  N +  +R KLL+E IAEIKAN+CE
Sbjct: 181 LVEEVRNLFDPNNVDYSHGIRKAIGVPELDRYLRASNSDYEIREKLLQEAIAEIKANTCE 240

Query: 246 LACRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLST 305
           LACRQ+ KILRLRNV GW IHRVDATEAFRK K K E +EAW K V+GPS +IV  FL+ 
Sbjct: 241 LACRQVDKILRLRNVKGWKIHRVDATEAFRK-KGK-EGDEAWTKLVAGPSALIVAHFLTQ 300

Query: 306 PTTHLQHQPIILKPKLFRMPLAAA 315
           P     H    LK K FRMP   A
Sbjct: 301 P-----HGQPYLKHKAFRMPFKRA 316

[Close]

BLAST of Lsi05G009240 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-79
Identity = 150/269 (55.76%), Postives = 199/269 (73.98%), Query Frame = 0


Query: 23  KEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPH 82
           K+KVVV+MG TG+GKS+LS+D+AT F  +EIINSDKIQ++QGLDI+TNKI+ E+  G+PH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRF-RAEIINSDKIQVHQGLDIVTNKITSEESCGVPH 99

Query: 83  HLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL-DHYSMFYSK 142
           HLLG+   P  D+    +CHMA+L+  S++ R  LPIIVGGSNSY+EAL+ D  + F S+
Sbjct: 100 HLLGV-LPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSR 159

Query: 143 YECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGIRKAIGVRELD 202
           Y+CC LWVDVA+P+LH FVS+RVD+MV +G+VEEV+  FD +N DYS GI+KAIG  E D
Sbjct: 160 YDCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFD 219

Query: 203 PYLRN--YNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWNIHRVDATEAF 262
            + RN  + N+  R +LL + + EIK N+ ELACRQ +KI RLR V  W+I RVDAT  F
Sbjct: 220 RFFRNEQFLNVEDREELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 263 RKNKTKFEMEEAWAKHVSGPSIMIVDQFL 289
            K ++K +   AW + V+GPS   V +FL
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFL 306

[Close]

BLAST of Lsi05G009240 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-77
Identity = 157/299 (52.51%), Postives = 203/299 (67.89%), Query Frame = 0


Query: 21  KAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGI 80
           + K+KVV VMG TG+GKS+L+IDLAT FP +EI+NSDKIQ+Y+GLDI+TNK++PE+  G+
Sbjct: 30  RRKDKVVFVMGATGTGKSRLAIDLATRFP-AEIVNSDKIQVYKGLDIVTNKVTPEESLGV 89

Query: 81  PHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALLDHYSMFYS 140
           PHHLLG     + D     F   A  A  SIV+R  +PII GGSNSYIEAL++    F  
Sbjct: 90  PHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRL 149

Query: 141 KYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVDYSYGIRKAIGVREL 200
           +Y CC LWVDV+ P+LHSFVS+RVD+MV  GLV+EV+ +FDP++ DYS GIR+AIGV EL
Sbjct: 150 RYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPEL 209

Query: 201 DPYL----RNYNNLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRNVIGWNIHRVDAT 260
           D +L    RNY       +LL+  I +IK N+C LACRQL+KI RL     WN+HRVDAT
Sbjct: 210 DEFLRSEMRNY-PAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDAT 269

Query: 261 EAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFLSTPTTHLQHQPIILKPKLFRMPLAAAA 316
           E F +     E +EAW   V+ PS + V++FLS    H      IL P++  +P   AA
Sbjct: 270 EVFLRRGE--EADEAWDNSVAHPSALAVEKFLSYSDDHHLEGANILLPEISAVPPLPAA 324

[Close]

BLAST of Lsi05G009240 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 146/283 (51.59%), Postives = 199/283 (70.32%), Query Frame = 0


Query: 8   SNLGMVNHNYLEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDI 67
           + L  VN N   +  KEKV+ VMG TGSGKS+L+IDLAT F   EIINSDKIQLY+GLD+
Sbjct: 19  NKLSKVNVNSF-LHPKEKVIFVMGATGSGKSRLAIDLATRF-QGEIINSDKIQLYKGLDV 78

Query: 68  LTNKISPEDQRGIPHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSY 127
           LTNK++P++ RG+PHHLLG+  +   ++  T++  +AS A + +   + LPI+ GGSNSY
Sbjct: 79  LTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSY 138

Query: 128 IEALLDHYSMF-YSKYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDPNNVD 187
           IEAL++H S F  + Y+CC +WVDV++P+L+SFVS RVD+M+  GL+EEV+ +F+P   +
Sbjct: 139 IEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNP-KAN 198

Query: 188 YSYGIRKAIGVRELDPYLRNYN--NLVLRNKLLKEGIAEIKANSCELACRQLKKILRLRN 247
           YS GIR+AIGV EL  YLRN +  +   ++K+L   +  IK N+  LACRQLKKI RL  
Sbjct: 199 YSVGIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHK 258

Query: 248 VIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQF 288
               ++HRVDATE F K   + E +EAW   V+ PS  IVD+F
Sbjct: 259 KWKMSMHRVDATEVFLKRNVE-EQDEAWENLVARPSERIVDKF 297

[Close]

BLAST of Lsi05G009240 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 221.5 bits (563), Expect = 9.8e-58
Identity = 125/293 (42.66%), Postives = 183/293 (62.46%), Query Frame = 0


Query: 23  KEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQRGIPH 82
           K+KVVV++G TG+GKS+LS+DLAT FP SEIINSDKIQ+Y+GL+I TN+I+ +D+RG+PH
Sbjct: 64  KDKVVVILGATGAGKSRLSVDLATRFP-SEIINSDKIQVYEGLEITTNQITLQDRRGVPH 123

Query: 83  HLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL-----DHYSM 142
           HLLG+    H ++   +F   AS     I  R  +PII GGSNS++ ALL       +  
Sbjct: 124 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 183

Query: 143 FYS---------KYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDP--NNVD 202
           F S         +YECC +WVDV+  +L+ ++  RVD+M+ +G+ EE+   +DP  + ++
Sbjct: 184 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 243

Query: 203 YSYGIRKAIGVRELDPYLRNY---NNLV----LRNKLLKEGIAEIKANSCELACRQLKKI 262
             +GIRKAIGV E D Y + Y     ++    LR     + + +IK N+  LA RQ+KKI
Sbjct: 244 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 303

Query: 263 LRLRNVIGWNIHRVDATEAFR----KNKTKFEMEEAWAKHVSGPSIMIVDQFL 289
             L++  GW I RVDAT +F+    K+ ++ +  E W + V  PS+ IV + L
Sbjct: 304 EMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

[Close]

BLAST of Lsi05G009240 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 201.4 bits (511), Expect = 1.0e-51
Identity = 121/296 (40.88%), Postives = 175/296 (59.12%), Query Frame = 0


Query: 18  LEVKAKEKVVVVMGPTGSGKSKLSIDLATHFPNSEIINSDKIQLYQGLDILTNKISPEDQ 77
           +E   K+KVVV+MG TGSGKS LSIDLAT F + EI+NSDKIQ Y GL + TN++S  ++
Sbjct: 37  MEQSYKQKVVVIMGATGSGKSCLSIDLATRF-SGEIVNSDKIQFYDGLKVTTNQMSILER 96

Query: 78  RGIPHHLLGITTTPHLDIPVTKFCHMASLAAASIVRRHHLPIIVGGSNSYIEALL----D 137
            G+PHHLLG       ++  ++F  +AS + + I  R +LPII GGSNS+I ALL    D
Sbjct: 97  CGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFD 156

Query: 138 HYSMFYS---------KYECCLLWVDVAMPLLHSFVSDRVDQMVANGLVEEVQGLFDP-- 197
             +  +S         +YECC LWVDV++ +L  ++S RVDQM+ +G+ EE+ G +DP  
Sbjct: 157 PKTYPFSSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRY 216

Query: 198 -NNVDYSYGIRKAIGVRELDPYLRNYNNL---------VLRNKLLKEGIAEIKANSCELA 257
             +   ++GI K IG+ E D Y   Y              R     E + EIK N+  LA
Sbjct: 217 SGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLA 276

Query: 258 CRQLKKILRLRNVIGWNIHRVDATEAFRKNKTKFEMEEAWAKHVSGPSIMIVDQFL 289
            +Q+++I++L++  GW+I R+DAT +F ++       E W   V   SI +V +FL
Sbjct: 277 KKQIERIMKLKS-SGWDIQRLDATPSFGRSS-----REIWDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:

BLAST of Lsi05G009240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC9	1.7e-78	55.76	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID2	3.5e-76	52.51	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	2.2e-70	51.59	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q94ID3	1.4e-56	42.66	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	2.6e-55	42.19	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]

BLAST of Lsi05G009240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HJ23	1.0e-107	67.59	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1HUH5	3.4e-106	66.16	adenylate isopentenyltransferase 3, chloroplastic OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6P9FA20	8.1e-84	57.86	adenylate isopentenyltransferase 3, chloroplastic OS=Juglans regia OX=51240 GN=L...	[more]
A0A5N6QRB0	1.4e-83	60.93	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_006115 PE=4 SV=1	[more]
A0A6A1UVG0	1.8e-83	58.31	Adenylate isopentenyltransferase 3, chloroplastic OS=Morella rubra OX=262757 GN=...	[more]

BLAST of Lsi05G009240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887422.1	2.9e-136	77.64	adenylate isopentenyltransferase 3, chloroplastic-like [Benincasa hispida] >XP_0...	[more]
KAG7011242.1	2.5e-108	67.47	Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper...	[more]
KAG6571479.1	4.3e-108	67.17	Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_023553600.1	9.7e-108	67.17	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]
XP_022963833.1	2.2e-107	67.59	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of Lsi05G009240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63110.1	1.2e-79	55.76	isopentenyltransferase 3	[more]
AT5G19040.1	2.5e-77	52.51	isopentenyltransferase 5	[more]
AT3G23630.1	1.6e-71	51.59	isopentenyltransferase 7	[more]
AT1G68460.1	9.8e-58	42.66	isopentenyltransferase 1	[more]
AT3G19160.1	1.0e-51	40.88	ATP/ADP isopentenyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 153..250 e-value: 4.0E-104 score: 349.6
None	No IPR available	PFAM	PF01715	IPPT	coord: 60..135 e-value: 8.3E-19 score: 68.3 coord: 141..237 e-value: 1.9E-12 score: 47.4
None	No IPR available	PANTHER	PTHR11088:SF74	ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC	coord: 17..292
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 23..288 e-value: 4.0E-104 score: 349.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 27..139
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 17..292

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G009240.1	Lsi05G009240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity