Lsi05G009010 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G009010
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProteinase inhibitor 1
Locationchr05: 16079539 .. 16080452 (+)
RNA-Seq ExpressionLsi05G009010
SyntenyLsi05G009010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTACATAGTATTTGTTACTTGTCGTCTAAAAGAATAACAAATACATAGTGTTTTTTTCCAATATTTTTAAATATACTTCTTTTTTGTTTTATCTCATGTAAAATGGTGTTGATAATGACTACTTGTTGGATAATTTTGTTTAGCTTTGGTGGTCAAATTGAGAAACACTAAACTCTACCATTTAAAAAAAAGGAAAATCCCAATGTCTTTCTAAAATCTAGAATAATCTTTTAAGTTTTAGAAGATAAACATAACAATATTCCATTTTAAAAAAAAAAAAAATCTAAATATGATTTTTTAAATAAAGTCCATATTCGTATTTAAAGATTTGAAAGATAAACATAAAAAATGGTAATCTTTTTTTAAATCGTGTTTACAAATTTGAAACATAGACACACATCTTTTTCAAAAAGAAGGAATGCATACGGTTATTCCTTACCATTTTCATTTATTTATTAATCTGGTAGAATTAACCTAACACAATTTATTTTTTATTTTATTGACATCATGTGACCAAACTAACTATAAATATAGCTACACAATCAACAACAAAGCACATCCCACTCAACTTGTAACAAATAGACAGATAACAAGTCCAAGATGACGTATCATTGTAAGTATAGCTTTTTGTAATATTTTGGTTGAGTTCATCTTCCTTTGTAAATATACATTTAATCTTAATTACTAAACAAATTATTTTTGCATATGTTTGAAAATAGTCAAATCATGGCCAGAACTCAACCTCATAGATGCAAACACTGCAGCAAATATCATCAAGAAAGAACATCCTAACTTCAATCCAGTCAAAGTGTTGGCAGGAAGTCCGGTAACACTAGATCTTCGACAAGGTCGAGTTCGGCTATTTGTTAATATAAAGGAAATAGTGGTTGAAGTTCCTCAAGAGGGCTGA

mRNA sequence

ATGGTCAAATCATGGCCAGAACTCAACCTCATAGATGCAAACACTGCAGCAAATATCATCAAGAAAGAACATCCTAACTTCAATCCAGTCAAAGTGTTGGCAGGAAGTCCGGTAACACTAGATCTTCGACAAGGTCGAGTTCGGCTATTTGTTAATATAAAGGAAATAGTGGTTGAAGTTCCTCAAGAGGGCTGA

Coding sequence (CDS)

ATGGTCAAATCATGGCCAGAACTCAACCTCATAGATGCAAACACTGCAGCAAATATCATCAAGAAAGAACATCCTAACTTCAATCCAGTCAAAGTGTTGGCAGGAAGTCCGGTAACACTAGATCTTCGACAAGGTCGAGTTCGGCTATTTGTTAATATAAAGGAAATAGTGGTTGAAGTTCCTCAAGAGGGCTGA

Protein sequence

MVKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQEG
Homology
BLAST of Lsi05G009010 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.2e-09
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 5   WPELNLIDANTAANIIKKEHPNFNPVK-VLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 64
           WPEL    A  A  II+KE+P    V+ VL G+PVT DLR  RVRLFVN+ + VV+ PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

BLAST of Lsi05G009010 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.4e-08
Identity = 31/61 (50.82%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 5   WPELNLIDANTAANIIKKEHPNFNPVK-VLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 64
           WPEL    A  A  II+KE+P    V+ +L G PVT DLR  RVRLFVN+ + +V+ PQ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQTPQI 106

BLAST of Lsi05G009010 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.1e-08
Identity = 31/63 (49.21%), Postives = 41/63 (65.08%), Query Frame = 0

Query: 3  KSWPELNLIDANTAANIIKKEHPNF-NPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 62
          ++WPEL  + A  A  II+KE+    N   VL GSPVT D R  RVRLFVN+ + VV++P
Sbjct: 32 ETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIP 91

Query: 63 QEG 65
          + G
Sbjct: 92 RVG 94

BLAST of Lsi05G009010 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.1e-08
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 0

Query: 3   KSWPELNLIDANTAANIIKKEHPNFNPVK-VLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 62
           +SWPEL    A  A  II+KE+P    V+ +L GS  T DLR  RVRLFVN+ +IVV+ P
Sbjct: 57  ESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTP 116

Query: 63  QEG 65
           + G
Sbjct: 117 KVG 119

BLAST of Lsi05G009010 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.5e-07
Identity = 30/59 (50.85%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 5   WPELNLIDANTAANIIKKEHPNFNPVKVLA-GSPVTLDLRQGRVRLFVNIKEIVVEVPQ 63
           WPEL  + A  A  II+KE+P+   + +L  GSPVT D R  RVRLFVNI   VV++P+
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

BLAST of Lsi05G009010 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.7e-15
Identity = 43/63 (68.25%), Postives = 51/63 (80.95%), Query Frame = 0

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP LN +DA T ANIIKKE+P+F    +LAGSPVT DL+ GRVRLF N++EIVV VP
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 62 QEG 65
          QEG
Sbjct: 69 QEG 71

BLAST of Lsi05G009010 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.0e-09
Identity = 36/63 (57.14%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP L  +DA T AN IK E+PNF  V V  G+PVT D+  GRVRL V+    VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 62 QEG 65
          QEG
Sbjct: 67 QEG 69

BLAST of Lsi05G009010 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.8e-09
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 0

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 63
          SWPEL  + ++ A + I+++ P+  PV +LAGSPVT DLR  RVR+FVN+ + VVEVP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 64 G 65
          G
Sbjct: 75 G 75

BLAST of Lsi05G009010 vs. ExPASy TrEMBL
Match: A0A6J1CY53 (inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=LOC111015335 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.5e-08
Identity = 31/62 (50.00%), Postives = 43/62 (69.35%), Query Frame = 0

Query: 3  KSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQ 62
          + WPEL  +  + AA IIK+E+    P  +LAGSPVTLD R  RVR+F NI+++VV +P+
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 63 EG 65
           G
Sbjct: 78 TG 79

BLAST of Lsi05G009010 vs. ExPASy TrEMBL
Match: A0A397ZK38 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-07
Identity = 31/63 (49.21%), Postives = 44/63 (69.84%), Query Frame = 0

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +K+WPEL     + AA++IK+E+P+FN   +L GS VT DLR+ RVR++V+   IVV VP
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 62 QEG 65
            G
Sbjct: 63 TTG 65

BLAST of Lsi05G009010 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-15
Identity = 43/64 (67.19%), Postives = 53/64 (82.81%), Query Frame = 0

Query: 1  MVKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEV 60
          ++KSWPELNL+ A+T  NIIK++HP F  V +LAGSPVT DL+ GRVRLFVNI  IVVE+
Sbjct: 4  VLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEI 63

Query: 61 PQEG 65
          P+EG
Sbjct: 64 PREG 67

BLAST of Lsi05G009010 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 89.7 bits (221), Expect = 9.6e-15
Identity = 43/63 (68.25%), Postives = 51/63 (80.95%), Query Frame = 0

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP LN +DA T ANIIKKE+P+F    +LAGSPVT DL+ GRVRLF N++EIVV VP
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 62 QEG 65
          QEG
Sbjct: 69 QEG 71

BLAST of Lsi05G009010 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-09
Identity = 36/63 (57.14%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 2  VKSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVP 61
          +KSWP L  +DA T AN IK E+PNF  V V  G+PVT D+  GRVRL V+    VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 62 QEG 65
          QEG
Sbjct: 67 QEG 69

BLAST of Lsi05G009010 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 70.1 bits (170), Expect = 7.9e-09
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 0

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 63
          SWPEL  + ++ A + I+++ P+  PV +LAGSPVT DLR  RVR+FVN+ + VVEVP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 64 G 65
          G
Sbjct: 75 G 75

BLAST of Lsi05G009010 vs. NCBI nr
Match: XP_022146026.1 (inhibitor of trypsin and hageman factor-like [Momordica charantia])

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-07
Identity = 31/62 (50.00%), Postives = 43/62 (69.35%), Query Frame = 0

Query: 3  KSWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQ 62
          + WPEL  +  + AA IIK+E+    P  +LAGSPVTLD R  RVR+F NI+++VV +P+
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 63 EG 65
           G
Sbjct: 78 TG 79

BLAST of Lsi05G009010 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 60.5 bits (145), Expect = 5.8e-10
Identity = 29/61 (47.54%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 4  SWPELNLIDANTAANIIKKEHPNFNPVKVLAGSPVTLDLRQGRVRLFVNIKEIVVEVPQE 63
          SWPEL   + + AA +I++E+P  N   +L GSPVT D R  RVR+FV+   IVV+ P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 64 G 65
          G
Sbjct: 70 G 70

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q031998.2e-0954.10Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1[more]
Q031982.4e-0850.82Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1[more]
Q022144.1e-0849.21Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1[more]
P200769.1e-0850.79Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ... [more]
P162313.5e-0750.85Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LIF64.7e-1568.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1[more]
A0A0A0LFD41.0e-0957.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1[more]
A0A0A0KPI03.8e-0952.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1[more]
A0A6J1CY535.5e-0850.00inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A397ZK382.1e-0749.21Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6571807.11.9e-1567.19hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN60477.19.6e-1568.25hypothetical protein Csa_001998 [Cucumis sativus][more]
KGN60478.12.1e-0957.14hypothetical protein Csa_001396 [Cucumis sativus][more]
XP_011654473.17.9e-0952.46inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic... [more]
XP_022146026.11.1e-0750.00inhibitor of trypsin and hageman factor-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G38870.15.8e-1047.54Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 41..51
score: 60.08
coord: 4..17
score: 39.13
coord: 29..40
score: 41.67
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 4..62
e-value: 1.5E-15
score: 57.6
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 4..64
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 2..64
e-value: 7.8E-17
score: 63.4
NoneNo IPR availablePANTHERPTHR33091:SF83ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1coord: 4..64
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 3..64

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G009010.1Lsi05G009010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity