Lsi05G008450 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G008450
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPectin lyase-like superfamily protein
Locationchr05: 13870493 .. 13872788 (-)
RNA-Seq ExpressionLsi05G008450
SyntenyLsi05G008450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAATCAACTTCCCAACACAAAACCACCCATATTCTCATCTATTTCTCATTTTATGGCTCTTCACACAACCTCATTCATTCTCTTACACCTTCTTTTCTACTTTTCTTCTGCCTCCTTGTCCACCTTTAATGTAGTCGATTTCGGTGCCAAACCCGACACCAAAACCGACTCGTCGAAGGCCTTCGAGGCAGCTTGGGCTGGAGCTTGTCGTGCATCGACAACCGGGTCCATTTACGTCCCGAAGGGCAAATTCTATCTCTCAAGTACAACTTTTGATGGGCCTTGCAATAACAATGATATCACTATACGCATCGATGGCACACTCTTCGCTCCGTCAAACTATCGTGTGATTGCAAACTCTGAAAATTGGATCACTTTTCGACATGTTGATGGTGTTGGTGTCTTTGGCGGCGTTCTTGATGCTCAAGGATTTGGATTGTGGGCTTGCAAAAATTCCAAAAATTCTTGCCCCTCGGGAGCCACGGTAAATCATTTTCAATAATAAAAAAAAATTGTTAAATTTTATTTAAAAAAAATCATTTTTTTAAATTTCCATGTGGCAAATGTTGATTAGGTGTTGTCTCAGCTACACAATAGTTTTTTTCGCTTTTCAATTTTAATCTTTATACTTTTAAATATCTAATTGTAGTCCTATATTTTCAATAAATCAAAAACTCAGTCCCCAATGCTAGTTTATTGTTGCTTTTTTAAAACTTTGTTTTTATTATTAGCATTATTACAATAATTTTTAAAACATATTCACATATTATATTTTTTTACATGAAAATTATTATTATTATTTAACTAATTTGGTAAAAATTAATTTTCAAAGACTAAATTTAAGATTTATTTAAAGTATAGACTAAAATTAAAAAATAAAAAGTACAGGCTAAAATTGAACAAATTGAAAATATAGGACCAAAACAATATTTTAACCAATTTTTTTTTTCTTTTTACAAAAGTTACCATTTTTATTTATTTCCTTCTGCGGAGATTTGCTTTCTAATTACAAAACATTATTGAATGTTGTTTTTTGGTGCAGTCGTTGGAATTTTCCAATTCAAAAAACATTATAGTTAGTGGATTGACGTCGCTGAATAGTCAAATGTTCCACATAGTAATCAATGATTGTCAAAATGTAAAAATGCAAGGACTGAAGGTCTCGGCTGCCGGCAACAGCCCGAACACAGACGGCATTCACGTAGCACAATCTTCAGCGGTTACTATCCTCAACTCCATCATTGGCACTGGTGATGACTGCATTTCAATAGGTCCTGACACGTCGAACTTGTGGATTGAGAATGTTGCTTGTGGACCTGGCCATGGAATTAGGTACTAAATTTTCAAATTTTAATGAAGTTTATGTGTTAATTGAGATAATTAAACATATGATTTTTATTTGATGGGTTGGTGCAGCATTGGGAGTTTAGGAAGAGAAATGCAAGAAAATGGTGTTCAGAATGTAACAGTGAAATCTGCCACATTCACCAACACTCAAAATGGGGTCAGAATTAAAACATGGGGAAGGCCAAGCAAAGGATTTGCAAAAAACATTATTTTTAAAGATATTGTAATGGTCAATGTCGAAAATCCTATCATCATCGATCAAAATTATTGCCCCGAAAACAAAGGTTGTCCTGGACAGGTCTGTATTGACATTTTTTTTTTGTTATCCAACTTCAGCTTGCCACGTGCCAAACTATTATTTTTTTCTTTTAAATATTTTGTATCTTATTTATTTATTTATTTTTTACAAATAGGATTCTGGAGTTAAAATTAGCGATGTGAAATATCGAAGCATCCACGGAACATCAGCAACACAAGTTGCGATGAAATTCGATTGTAGCTCGAAATTTCCATGCAGTGACATAACGCTGGAGGATGTGAAGCTGAGTTATAAAAATGAAGCAGCTAAAGCTTCATGTAGTCATGCTGAAGGAAGCGTTGCTAGTTTAGTTCAACCATCAAGTTGTTTGTAGGGCAAATCTTAGAACTATATAGTATATAATTATATAATTATATTATATGGTTTTTTTTAAGGGTTAGGAATATGTTAGTTGTCTTGATATTGCTAACGATCAAAGTTCTAAGGTTGAGGTTATAGATTTAGTAACCTACTATAGGCAGTCATGTCGCGAATCAACATCGAGTTTTCTAGATGTTTGTTTTAGTTTAGTATATTCAACATTAGTCTACGAGTGTCAATCACTAGACACGACACTTTATTACTGTTTTAAATATTGGTGATTTGATTTTTACCATTATTTATTTATTGTTCGTTATTCAAATGGATT

mRNA sequence

CCCAATCAACTTCCCAACACAAAACCACCCATATTCTCATCTATTTCTCATTTTATGGCTCTTCACACAACCTCATTCATTCTCTTACACCTTCTTTTCTACTTTTCTTCTGCCTCCTTGTCCACCTTTAATGTAGTCGATTTCGGTGCCAAACCCGACACCAAAACCGACTCGTCGAAGGCCTTCGAGGCAGCTTGGGCTGGAGCTTGTCGTGCATCGACAACCGGGTCCATTTACGTCCCGAAGGGCAAATTCTATCTCTCAAGTACAACTTTTGATGGGCCTTGCAATAACAATGATATCACTATACGCATCGATGGCACACTCTTCGCTCCGTCAAACTATCGTGTGATTGCAAACTCTGAAAATTGGATCACTTTTCGACATGTTGATGGTGTTGGTGTCTTTGGCGGCGTTCTTGATGCTCAAGGATTTGGATTGTGGGCTTGCAAAAATTCCAAAAATTCTTGCCCCTCGGGAGCCACGTCGTTGGAATTTTCCAATTCAAAAAACATTATAGTTAGTGGATTGACGTCGCTGAATAGTCAAATGTTCCACATAGTAATCAATGATTGTCAAAATGTAAAAATGCAAGGACTGAAGGTCTCGGCTGCCGGCAACAGCCCGAACACAGACGGCATTCACGTAGCACAATCTTCAGCGGTTACTATCCTCAACTCCATCATTGGCACTGGTGATGACTGCATTTCAATAGGTCCTGACACGTCGAACTTGTGGATTGAGAATGTTGCTTGTGGACCTGGCCATGGAATTAGCATTGGGAGTTTAGGAAGAGAAATGCAAGAAAATGGTGTTCAGAATGTAACAGTGAAATCTGCCACATTCACCAACACTCAAAATGGGGTCAGAATTAAAACATGGGGAAGGCCAAGCAAAGGATTTGCAAAAAACATTATTTTTAAAGATATTGTAATGGTCAATGTCGAAAATCCTATCATCATCGATCAAAATTATTGCCCCGAAAACAAAGGTTGTCCTGGACAGGATTCTGGAGTTAAAATTAGCGATGTGAAATATCGAAGCATCCACGGAACATCAGCAACACAAGTTGCGATGAAATTCGATTGTAGCTCGAAATTTCCATGCAGTGACATAACGCTGGAGGATGTGAAGCTGAGTTATAAAAATGAAGCAGCTAAAGCTTCATGTAGTCATGCTGAAGGAAGCGTTGCTAGTTTAGTTCAACCATCAAGTTGTTTGTAGGGCAAATCTTAGAACTATATAGTATATAATTATATAATTATATTATATGGTTTTTTTTAAGGGTTAGGAATATGTTAGTTGTCTTGATATTGCTAACGATCAAAGTTCTAAGGTTGAGGTTATAGATTTAGTAACCTACTATAGGCAGTCATGTCGCGAATCAACATCGAGTTTTCTAGATGTTTGTTTTAGTTTAGTATATTCAACATTAGTCTACGAGTGTCAATCACTAGACACGACACTTTATTACTGTTTTAAATATTGGTGATTTGATTTTTACCATTATTTATTTATTGTTCGTTATTCAAATGGATT

Coding sequence (CDS)

ATGGCTCTTCACACAACCTCATTCATTCTCTTACACCTTCTTTTCTACTTTTCTTCTGCCTCCTTGTCCACCTTTAATGTAGTCGATTTCGGTGCCAAACCCGACACCAAAACCGACTCGTCGAAGGCCTTCGAGGCAGCTTGGGCTGGAGCTTGTCGTGCATCGACAACCGGGTCCATTTACGTCCCGAAGGGCAAATTCTATCTCTCAAGTACAACTTTTGATGGGCCTTGCAATAACAATGATATCACTATACGCATCGATGGCACACTCTTCGCTCCGTCAAACTATCGTGTGATTGCAAACTCTGAAAATTGGATCACTTTTCGACATGTTGATGGTGTTGGTGTCTTTGGCGGCGTTCTTGATGCTCAAGGATTTGGATTGTGGGCTTGCAAAAATTCCAAAAATTCTTGCCCCTCGGGAGCCACGTCGTTGGAATTTTCCAATTCAAAAAACATTATAGTTAGTGGATTGACGTCGCTGAATAGTCAAATGTTCCACATAGTAATCAATGATTGTCAAAATGTAAAAATGCAAGGACTGAAGGTCTCGGCTGCCGGCAACAGCCCGAACACAGACGGCATTCACGTAGCACAATCTTCAGCGGTTACTATCCTCAACTCCATCATTGGCACTGGTGATGACTGCATTTCAATAGGTCCTGACACGTCGAACTTGTGGATTGAGAATGTTGCTTGTGGACCTGGCCATGGAATTAGCATTGGGAGTTTAGGAAGAGAAATGCAAGAAAATGGTGTTCAGAATGTAACAGTGAAATCTGCCACATTCACCAACACTCAAAATGGGGTCAGAATTAAAACATGGGGAAGGCCAAGCAAAGGATTTGCAAAAAACATTATTTTTAAAGATATTGTAATGGTCAATGTCGAAAATCCTATCATCATCGATCAAAATTATTGCCCCGAAAACAAAGGTTGTCCTGGACAGGATTCTGGAGTTAAAATTAGCGATGTGAAATATCGAAGCATCCACGGAACATCAGCAACACAAGTTGCGATGAAATTCGATTGTAGCTCGAAATTTCCATGCAGTGACATAACGCTGGAGGATGTGAAGCTGAGTTATAAAAATGAAGCAGCTAAAGCTTCATGTAGTCATGCTGAAGGAAGCGTTGCTAGTTTAGTTCAACCATCAAGTTGTTTGTAG

Protein sequence

MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYKNEAAKASCSHAEGSVASLVQPSSCL
Homology
BLAST of Lsi05G008450 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.3e-149
Identity = 263/388 (67.78%), Postives = 307/388 (79.12%), Query Frame = 0

Query: 7   SFILLHLLFYF----SSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYV 66
           S   L L+F F    + AS  T+NV   GAK D KTDS+KAF +AWA AC +   G IYV
Sbjct: 6   SLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYV 65

Query: 67  PKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVL 126
           P G F+L    F GPC NN IT RI GTL APS+YRVI N+ NWI F HV+GV + GG+L
Sbjct: 66  PAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGIL 125

Query: 127 DAQGFGLWACKNSK-NSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQG 186
           D QG  LWACK     SCPSGAT+L FS+S NI+VSGL SLNSQMFHIVIND QNV+MQG
Sbjct: 126 DGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQG 185

Query: 187 LKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGIS 246
           ++VS +GNSPNTDGIHV  SS VTILNS I TGDDC+SIGP TSNLWIE VACGPGHGIS
Sbjct: 186 VRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGIS 245

Query: 247 IGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPI 306
           IGSLG+E +E GVQNVTVK+ TF+ TQNG+RIK+WGRPS GFA+NI+F+   MVNVENPI
Sbjct: 246 IGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPI 305

Query: 307 IIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKL 366
           +IDQ+YCP+NKGCPGQ SGV+ISDV Y  IHGTSAT+VA+KFDCS K PC +I LEDVKL
Sbjct: 306 VIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKL 365

Query: 367 SYKNEAAKASCSHAEGSVASLVQPSSCL 390
           +YKN+AA++SCSHA+G+   +VQP+SCL
Sbjct: 366 TYKNQAAESSCSHADGTTEGVVQPTSCL 393

BLAST of Lsi05G008450 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 3.1e-111
Identity = 204/369 (55.28%), Postives = 266/369 (72.09%), Query Frame = 0

Query: 23  STFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKGKFYLSSTTFDG-PCNNN 82
           ST NV+ +GAKPD   DS+KAF AAW  AC ++   +I VPKG+F + +  F G  C   
Sbjct: 31  STLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQA 90

Query: 83  DITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVLDAQGFGLWACKNS-KNSCP 142
            I+IRI G++ AP ++R+IA+S++WI F  V  V ++GG+LDAQG  LW CKN+  ++CP
Sbjct: 91  PISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCP 150

Query: 143 SGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVSAAGNSPNTDGIHVAQ 202
           +GA SL FS S NI +SGLTS+NSQ FHIVI++  NV + G+KVSA  NSPNTDGIHV  
Sbjct: 151 TGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVES 210

Query: 203 SSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSLGREMQENGVQNVTVK 262
           S +V I NS IGTGDDCISIGP ++N++I+ + CGPGHGISIGSLGR  +E GV NVTV 
Sbjct: 211 SHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVS 270

Query: 263 SATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQNYCPENKGCPGQDSG 322
           +  F  T NGVRIKTWG+ S  FA+NI+F+ I M  V+NPIIIDQ+YC  +K CP Q+SG
Sbjct: 271 NVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYC-LHKPCPKQESG 330

Query: 323 VKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYKNEAAKASCSHAEGSVA 382
           VK+S+V+Y  IHGTS T+VA+  DCS + PC+ I ++DV L   +  A+ASC +A GS  
Sbjct: 331 VKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSAN 390

Query: 383 SLVQPSSCL 390
            +V  + CL
Sbjct: 391 DVVPFTPCL 398

BLAST of Lsi05G008450 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.0e-77
Identity = 157/372 (42.20%), Postives = 225/372 (60.48%), Query Frame = 0

Query: 20  ASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKGKFYLSSTTFDGPCN 79
           +S S FNV D+GAK     D S+A   AW  AC +    ++ +PKG + +      GPC 
Sbjct: 5   SSGSVFNVNDYGAK--GAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCK 64

Query: 80  NNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFG-GVLDAQGFGLWACKN-SKN 139
            + I  +IDG + AP++      S+ W++F  +DG+ V G G LD QG   WA  N  KN
Sbjct: 65  GSKIGFQIDGVVKAPADPSKF-KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKN 124

Query: 140 -SCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVSAAGNSPNTDGI 199
            +C   A +L F   K+ +V  +TSLNS+MFHI + +C+++  Q + V+A G S NTDGI
Sbjct: 125 PNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGI 184

Query: 200 HVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSLGREMQENGVQN 259
           HV  S  VTI N+ I TGDDCISIGP + N+ I  V CGPGHGISIGSLGR   E  V+ 
Sbjct: 185 HVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRG 244

Query: 260 VTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQNYCPENKGCPG 319
           +TVK  TF+ T NGVR+KTW     G A ++ F+D+ M NV+NP+I+DQ YCP  +    
Sbjct: 245 ITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQ 304

Query: 320 QDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYKNEA--AKASCSH 379
             S +K+S++ + +I GTS  +VA+   CS   PCS++ + ++ LSY+     A ++CS+
Sbjct: 305 APSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSN 364

Query: 380 AEGSVASLVQPS 387
            + + +    P+
Sbjct: 365 VKPTFSGKQVPA 373

BLAST of Lsi05G008450 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 7.3e-76
Identity = 164/370 (44.32%), Postives = 225/370 (60.81%), Query Frame = 0

Query: 24  TFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKGKFYLSSTTFDGPCNNNDI 83
           T NV DFGAK D + D +KAFE AW  AC ++++  + VPK  + +   +F GPC +  +
Sbjct: 89  TVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG-L 148

Query: 84  TIRIDGTLFAP---SNYRVIANSENWITFRHVDGVGV-FGGVLDAQGFGLW--ACKNSKN 143
           T++I GT+ A    S+YR   +  +W+ F  V  + V  GG ++  G   W  +CK +K 
Sbjct: 149 TMQIYGTIEASDDRSDYR--KDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKA 208

Query: 144 -SCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVSAAGNSPNTDGI 203
             C    T+L F  SK++IV  L   N+Q  H+  ++C NV+   L V+A  NSPNTDGI
Sbjct: 209 LPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGI 268

Query: 204 HVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSLGREMQENGVQN 263
           HV  +  + I + +IGTGDDCISI   +  + + ++ CGPGHGISIGSLG    E  V +
Sbjct: 269 HVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSD 328

Query: 264 VTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQNYCPENKGCPG 323
           V V  A    T NGVRIKTW +   G A NI F+++ M NVENPIIIDQNYC ++K C  
Sbjct: 329 VVVNGAKLCGTTNGVRIKTW-QGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQE 388

Query: 324 QDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYK-NEAAKASCSHA 383
           Q S V++ +V Y++I GT A+ VA+ FDCS +FPC  I LEDV L  +   AAKA C++ 
Sbjct: 389 QSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNV 448

Query: 384 EGSVASLVQP 386
           E S   +V P
Sbjct: 449 ELSETGVVSP 453

BLAST of Lsi05G008450 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 283.5 bits (724), Expect = 3.6e-75
Identity = 165/379 (43.54%), Postives = 226/379 (59.63%), Query Frame = 0

Query: 16  YFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYV--PKGKFY-LSST 75
           Y   +S  +FNV  FGAK +   D SKAF  AW  AC  S+TG +Y+  PK + Y L + 
Sbjct: 62  YAPRSSPRSFNVNTFGAKAN-GNDDSKAFMKAWEAAC--SSTGIVYIVAPKNRDYMLKAV 121

Query: 76  TFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGV-FGGVLDAQGFGLW- 135
           TF GPC ++ I  +I G + A  N        +WI F +V+ + V  GG +D  G   W 
Sbjct: 122 TFSGPCKSSLIIFKIYGRIEAWENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWP 181

Query: 136 -ACK-NSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVSAAG 195
            +CK N +  C    T++ F    N+ VS +   N+Q  H+   DC+NVK   L V++  
Sbjct: 182 KSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPA 241

Query: 196 NSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSLGRE 255
           +SPNTDGIHV+ +  + I +SI+ TGDDCISI   + N+    + CGPGHGISIGSLG +
Sbjct: 242 DSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGED 301

Query: 256 MQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQNYC 315
             E  V NV V  AT   T NGVRIKTW +   G AKNIIF+DI+M NV NPIII+Q+YC
Sbjct: 302 NSEAYVSNVVVNKATLIGTTNGVRIKTW-QGGHGMAKNIIFQDIIMKNVTNPIIINQDYC 361

Query: 316 PENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKL--SYKNE 375
              + CP Q S V++S+V Y++I GTS+  +A+KF CS   PC  I++++VKL    + +
Sbjct: 362 DRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQD 421

Query: 376 AAKASCSHAEGSVASLVQP 386
            +KASCS+ +      V P
Sbjct: 422 VSKASCSNVKLDTRGNVSP 436

BLAST of Lsi05G008450 vs. ExPASy TrEMBL
Match: A0A5D3DV94 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00420 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 330/389 (84.83%), Postives = 356/389 (91.52%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSI 60
           MALH T FI   L  + + AS STFNVVDFGAKP+  TDSS+AFE AWA ACRA+T  SI
Sbjct: 1   MALHKTLFI---LFLFLTLASASTFNVVDFGAKPNIGTDSSQAFEEAWASACRATTAVSI 60

Query: 61  YVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGG 120
           YVPKG+FY+ S  F+GPCNNNDIT+RIDGTL APSNY VIANS NWITF+ VDGV VFGG
Sbjct: 61  YVPKGRFYVKSIAFEGPCNNNDITVRIDGTLLAPSNYAVIANSGNWITFKRVDGVNVFGG 120

Query: 121 VLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQ 180
           VLDAQGFGLWACKNSK+SCPSGATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVK Q
Sbjct: 121 VLDAQGFGLWACKNSKSSCPSGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQ 180

Query: 181 GLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGI 240
           GLK+SAAGNSPNTDGIHVA SS VTILNSIIGTGDDCISIGP TSNLWIENVACGPGHGI
Sbjct: 181 GLKISAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGI 240

Query: 241 SIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENP 300
           SIGSLGRE+QE+GV+NVTVKSATFTNTQNGVRIKTWG+PS GFA+++IF+DIVMVNVENP
Sbjct: 241 SIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENP 300

Query: 301 IIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360
           IIIDQNYCP+NKGCPGQDSGVKISDV YR+IHGTSATQVAMKFDCSSKFPC+DITLEDV+
Sbjct: 301 IIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCNDITLEDVE 360

Query: 361 LSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           LSYKNEAA+ASCSHAEGS A LVQPSSCL
Sbjct: 361 LSYKNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Lsi05G008450 vs. ExPASy TrEMBL
Match: A0A5A7ULI9 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold319G001680 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.3e-188
Identity = 330/389 (84.83%), Postives = 356/389 (91.52%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSI 60
           MALH T FI   L  + + AS STFNVVDFGAKP+  TDSS+AFE AWA ACRA+T  SI
Sbjct: 1   MALHKTLFI---LFLFLTLASASTFNVVDFGAKPNIGTDSSQAFEEAWASACRATTAVSI 60

Query: 61  YVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGG 120
           YVPKG+FY+ S  F+GPCNNNDITIRIDGTL APSNY VIA+S NWITF+ VDGV VFGG
Sbjct: 61  YVPKGRFYVKSIAFEGPCNNNDITIRIDGTLLAPSNYAVIADSGNWITFKRVDGVNVFGG 120

Query: 121 VLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQ 180
           VLDAQGFGLWACKNSK+SCPSGATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVK Q
Sbjct: 121 VLDAQGFGLWACKNSKSSCPSGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQ 180

Query: 181 GLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGI 240
           GLK+SAAGNSPNTDGIHVA SS VTILNSIIGTGDDCISIGP TSNLWIENVACGPGHGI
Sbjct: 181 GLKISAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGI 240

Query: 241 SIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENP 300
           SIGSLGRE+QE+GV+NVTVKSATFTNTQNGVRIKTWG+PS GFA+++IF+DIVMVNVENP
Sbjct: 241 SIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENP 300

Query: 301 IIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360
           IIIDQNYCP+NKGCPGQDSGVKISDV YR+IHGTSATQVAMKFDCSSKFPC+DITLEDV+
Sbjct: 301 IIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCNDITLEDVE 360

Query: 361 LSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           LSYKNEAA+ASCSHAEGS A LVQPSSCL
Sbjct: 361 LSYKNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Lsi05G008450 vs. ExPASy TrEMBL
Match: A0A0A0M095 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627460 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.3e-186
Identity = 329/389 (84.58%), Postives = 352/389 (90.49%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSI 60
           MA H T F       + + AS STFNVVDFGAKP+ +TDSS+AFEAAWA ACRA T  SI
Sbjct: 1   MAFHKTLFF---PFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSI 60

Query: 61  YVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGG 120
           YVPKG+FY++S  F+GPCNNNDITIRIDGTL APSNY VIANS NWITFR VDGV +FGG
Sbjct: 61  YVPKGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGG 120

Query: 121 VLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQ 180
           VLDAQG GLWACKNSK+SCP GATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVK Q
Sbjct: 121 VLDAQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQ 180

Query: 181 GLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGI 240
           GLKVSAAGNSPNTDGIHVA SS VTILNSIIGTGDDCISIGP TSNLWIENVACGPGHGI
Sbjct: 181 GLKVSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGI 240

Query: 241 SIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENP 300
           SIGSLGRE+QE+GV+NVTVKSATFTNTQNGVRIKTWG+PS GFA+++IF+DIVMVNVENP
Sbjct: 241 SIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENP 300

Query: 301 IIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360
           IIIDQNYCP+NKGCPGQDSGVKISDV YR+IHGTSATQVAMKFDCSSKFPCSDITLEDVK
Sbjct: 301 IIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360

Query: 361 LSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           LS KNEAA+ASCSHAEGS A LVQPSSCL
Sbjct: 361 LSCKNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Lsi05G008450 vs. ExPASy TrEMBL
Match: A0A6J1EYE0 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439559 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.7e-174
Identity = 304/391 (77.75%), Postives = 345/391 (88.24%), Query Frame = 0

Query: 1   MALHTTSFILLHL--LFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTG 60
           MA+ +T FILL L   F+F S S +TFN+VDFGAKPD+KTD SKAFE AW  ACRAST  
Sbjct: 1   MAIPSTPFILLPLFFFFFFFSVSSTTFNIVDFGAKPDSKTDMSKAFEEAWTSACRASTAA 60

Query: 61  SIYVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVF 120
           +IYVPKG++YLS+TTF+GPC+NNDIT+ +DGTL APS+Y V    ENWITFRHVDGV VF
Sbjct: 61  TIYVPKGRYYLSTTTFNGPCSNNDITMHVDGTLVAPSDYGV----ENWITFRHVDGVSVF 120

Query: 121 GGVLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVK 180
           GGVLD QG GLW CKNS  SCP GATSLEFSNSKNIIVSGLTSLNSQMFHIV N CQNVK
Sbjct: 121 GGVLDGQGAGLWDCKNSHKSCPLGATSLEFSNSKNIIVSGLTSLNSQMFHIVANGCQNVK 180

Query: 181 MQGLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGH 240
           MQG+K+SAAGNSPNTDGIHVA SS VTI++S I TGDDCIS+GP TSNLWIENVACGPGH
Sbjct: 181 MQGIKISAAGNSPNTDGIHVALSSQVTIISSSISTGDDCISVGPGTSNLWIENVACGPGH 240

Query: 241 GISIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVE 300
           GISIGSLG++++E+GVQNVTVK+ TFTNTQNGVRIKTWG+PSKGFA+NI+F+DI+M NV+
Sbjct: 241 GISIGSLGKDVEEDGVQNVTVKTTTFTNTQNGVRIKTWGKPSKGFARNILFQDIIMNNVQ 300

Query: 301 NPIIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLED 360
           NPIIIDQNYCP NKGCPGQ SGV+IS+V Y++IHGTSATQVAMKFDCSSKFPC  ITLED
Sbjct: 301 NPIIIDQNYCPNNKGCPGQVSGVEISNVSYQNIHGTSATQVAMKFDCSSKFPCRGITLED 360

Query: 361 VKLSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           VKLSYKN+ A+ASCS+A+G+ A LVQPSSCL
Sbjct: 361 VKLSYKNDVAEASCSNADGTAAGLVQPSSCL 387

BLAST of Lsi05G008450 vs. ExPASy TrEMBL
Match: A0A6J1DRR7 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023699 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.7e-163
Identity = 287/390 (73.59%), Postives = 336/390 (86.15%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFS-SASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGS 60
           MAL  +  ++L L    S +AS STF++VDFGAK D +TDSSKA EAAW+ AC++S   S
Sbjct: 1   MALPISLLLILFLSLNSSLAASSSTFDIVDFGAKSDGETDSSKALEAAWSNACKSSAAAS 60

Query: 61  IYVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFG 120
           +YVPKGKFY+SS TF+G CNNN IT+RIDGTL APS++R I NS+NWI FR VDGV V G
Sbjct: 61  VYVPKGKFYVSSATFEGHCNNNAITVRIDGTLVAPSDFRAIGNSDNWIIFRLVDGVTVLG 120

Query: 121 GVLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKM 180
           G+LDAQG  LWACK S  +CPSGATSLEFSNSKNIIV+GLTSLNSQMFHIV+N+CQNVKM
Sbjct: 121 GILDAQGTALWACKKSGQNCPSGATSLEFSNSKNIIVNGLTSLNSQMFHIVVNECQNVKM 180

Query: 181 QGLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHG 240
            G+K+SA G+SPNTDGIHV +SS VT+++S IGTGDDCISIGP TSNLWIENVACGPGHG
Sbjct: 181 HGIKISAPGDSPNTDGIHVQRSSDVTVIHSNIGTGDDCISIGPGTSNLWIENVACGPGHG 240

Query: 241 ISIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVEN 300
           ISIGSLG+E QE GVQNVTVKSATFT+T+NGVRIKTWGRPS GFAKNIIF+DI+M+NV+N
Sbjct: 241 ISIGSLGKEEQEAGVQNVTVKSATFTDTKNGVRIKTWGRPSNGFAKNIIFQDILMLNVQN 300

Query: 301 PIIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDV 360
           PIIIDQNYCP  + CPGQDSGVKIS+V Y++IHG SA++VA+KFDCS KFPCS I +EDV
Sbjct: 301 PIIIDQNYCPHAQACPGQDSGVKISNVTYKNIHGISASEVAVKFDCSPKFPCSGIRVEDV 360

Query: 361 KLSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           +LS +NEAA+ASCSHA G+ A L+QPSSCL
Sbjct: 361 RLSCQNEAAEASCSHAGGTAAGLLQPSSCL 390

BLAST of Lsi05G008450 vs. NCBI nr
Match: XP_038888709.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 688.3 bits (1775), Expect = 3.7e-194
Identity = 340/390 (87.18%), Postives = 359/390 (92.05%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYF-SSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGS 60
           MALHT SFI LH LF F SS S STFNVVDFGAKPD KTDSSKAFEAAW GACRAST GS
Sbjct: 1   MALHTISFIFLHFLFLFLSSVSSSTFNVVDFGAKPDAKTDSSKAFEAAWVGACRASTAGS 60

Query: 61  IYVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFG 120
           IYVPKG+FYL+S TFDGPCNNNDITI IDGTL APSNY VIAN+ NWITFRHV GV VFG
Sbjct: 61  IYVPKGRFYLTSATFDGPCNNNDITIHIDGTLLAPSNYGVIANNGNWITFRHVYGVSVFG 120

Query: 121 GVLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKM 180
           G+LDAQGF LWACK+SKNSCPSGATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVKM
Sbjct: 121 GILDAQGFELWACKHSKNSCPSGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKM 180

Query: 181 QGLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHG 240
           QGLK+SAA +SPNTDGIHVA SS VTILNSIIGTGDDCIS+GP TSNLWIENVACGPGHG
Sbjct: 181 QGLKISAASDSPNTDGIHVALSSVVTILNSIIGTGDDCISVGPSTSNLWIENVACGPGHG 240

Query: 241 ISIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVEN 300
           ISIGSLGREMQE+GVQNVTVKSA+FTNTQNGVRIKTWG+PSKGFA+NI+F+DIVMVNVEN
Sbjct: 241 ISIGSLGREMQEDGVQNVTVKSASFTNTQNGVRIKTWGKPSKGFARNILFQDIVMVNVEN 300

Query: 301 PIIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDV 360
           PIIIDQNYCP NK CPGQDSGVKISDV YR+IHGTSAT+VAMKFDCSSKFPCSDI LEDV
Sbjct: 301 PIIIDQNYCPNNKNCPGQDSGVKISDVTYRNIHGTSATEVAMKFDCSSKFPCSDIMLEDV 360

Query: 361 KLSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           KLSYKN+AA+ASCSHAEG    LVQPSSCL
Sbjct: 361 KLSYKNKAAEASCSHAEGGATGLVQPSSCL 390

BLAST of Lsi05G008450 vs. NCBI nr
Match: TYK27707.1 (polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 669.1 bits (1725), Expect = 2.3e-188
Identity = 330/389 (84.83%), Postives = 356/389 (91.52%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSI 60
           MALH T FI   L  + + AS STFNVVDFGAKP+  TDSS+AFE AWA ACRA+T  SI
Sbjct: 1   MALHKTLFI---LFLFLTLASASTFNVVDFGAKPNIGTDSSQAFEEAWASACRATTAVSI 60

Query: 61  YVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGG 120
           YVPKG+FY+ S  F+GPCNNNDIT+RIDGTL APSNY VIANS NWITF+ VDGV VFGG
Sbjct: 61  YVPKGRFYVKSIAFEGPCNNNDITVRIDGTLLAPSNYAVIANSGNWITFKRVDGVNVFGG 120

Query: 121 VLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQ 180
           VLDAQGFGLWACKNSK+SCPSGATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVK Q
Sbjct: 121 VLDAQGFGLWACKNSKSSCPSGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQ 180

Query: 181 GLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGI 240
           GLK+SAAGNSPNTDGIHVA SS VTILNSIIGTGDDCISIGP TSNLWIENVACGPGHGI
Sbjct: 181 GLKISAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGI 240

Query: 241 SIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENP 300
           SIGSLGRE+QE+GV+NVTVKSATFTNTQNGVRIKTWG+PS GFA+++IF+DIVMVNVENP
Sbjct: 241 SIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENP 300

Query: 301 IIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360
           IIIDQNYCP+NKGCPGQDSGVKISDV YR+IHGTSATQVAMKFDCSSKFPC+DITLEDV+
Sbjct: 301 IIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCNDITLEDVE 360

Query: 361 LSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           LSYKNEAA+ASCSHAEGS A LVQPSSCL
Sbjct: 361 LSYKNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Lsi05G008450 vs. NCBI nr
Match: KAA0056020.1 (polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 667.5 bits (1721), Expect = 6.8e-188
Identity = 330/389 (84.83%), Postives = 356/389 (91.52%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSI 60
           MALH T FI   L  + + AS STFNVVDFGAKP+  TDSS+AFE AWA ACRA+T  SI
Sbjct: 1   MALHKTLFI---LFLFLTLASASTFNVVDFGAKPNIGTDSSQAFEEAWASACRATTAVSI 60

Query: 61  YVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGG 120
           YVPKG+FY+ S  F+GPCNNNDITIRIDGTL APSNY VIA+S NWITF+ VDGV VFGG
Sbjct: 61  YVPKGRFYVKSIAFEGPCNNNDITIRIDGTLLAPSNYAVIADSGNWITFKRVDGVNVFGG 120

Query: 121 VLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQ 180
           VLDAQGFGLWACKNSK+SCPSGATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVK Q
Sbjct: 121 VLDAQGFGLWACKNSKSSCPSGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQ 180

Query: 181 GLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGI 240
           GLK+SAAGNSPNTDGIHVA SS VTILNSIIGTGDDCISIGP TSNLWIENVACGPGHGI
Sbjct: 181 GLKISAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGI 240

Query: 241 SIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENP 300
           SIGSLGRE+QE+GV+NVTVKSATFTNTQNGVRIKTWG+PS GFA+++IF+DIVMVNVENP
Sbjct: 241 SIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENP 300

Query: 301 IIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360
           IIIDQNYCP+NKGCPGQDSGVKISDV YR+IHGTSATQVAMKFDCSSKFPC+DITLEDV+
Sbjct: 301 IIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCNDITLEDVE 360

Query: 361 LSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           LSYKNEAA+ASCSHAEGS A LVQPSSCL
Sbjct: 361 LSYKNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Lsi05G008450 vs. NCBI nr
Match: XP_004139060.1 (polygalacturonase [Cucumis sativus] >KGN66537.1 hypothetical protein Csa_007593 [Cucumis sativus])

HSP 1 Score: 660.2 bits (1702), Expect = 1.1e-185
Identity = 329/389 (84.58%), Postives = 352/389 (90.49%), Query Frame = 0

Query: 1   MALHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSI 60
           MA H T F       + + AS STFNVVDFGAKP+ +TDSS+AFEAAWA ACRA T  SI
Sbjct: 1   MAFHKTLFF---PFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSI 60

Query: 61  YVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGG 120
           YVPKG+FY++S  F+GPCNNNDITIRIDGTL APSNY VIANS NWITFR VDGV +FGG
Sbjct: 61  YVPKGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGG 120

Query: 121 VLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQ 180
           VLDAQG GLWACKNSK+SCP GATSLEFSNSKNI+VSGLTSLNSQMFHIVIN CQNVK Q
Sbjct: 121 VLDAQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQ 180

Query: 181 GLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGI 240
           GLKVSAAGNSPNTDGIHVA SS VTILNSIIGTGDDCISIGP TSNLWIENVACGPGHGI
Sbjct: 181 GLKVSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGI 240

Query: 241 SIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENP 300
           SIGSLGRE+QE+GV+NVTVKSATFTNTQNGVRIKTWG+PS GFA+++IF+DIVMVNVENP
Sbjct: 241 SIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENP 300

Query: 301 IIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360
           IIIDQNYCP+NKGCPGQDSGVKISDV YR+IHGTSATQVAMKFDCSSKFPCSDITLEDVK
Sbjct: 301 IIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVK 360

Query: 361 LSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           LS KNEAA+ASCSHAEGS A LVQPSSCL
Sbjct: 361 LSCKNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Lsi05G008450 vs. NCBI nr
Match: XP_022932944.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 621.3 bits (1601), Expect = 5.6e-174
Identity = 304/391 (77.75%), Postives = 345/391 (88.24%), Query Frame = 0

Query: 1   MALHTTSFILLHL--LFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTG 60
           MA+ +T FILL L   F+F S S +TFN+VDFGAKPD+KTD SKAFE AW  ACRAST  
Sbjct: 1   MAIPSTPFILLPLFFFFFFFSVSSTTFNIVDFGAKPDSKTDMSKAFEEAWTSACRASTAA 60

Query: 61  SIYVPKGKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVF 120
           +IYVPKG++YLS+TTF+GPC+NNDIT+ +DGTL APS+Y V    ENWITFRHVDGV VF
Sbjct: 61  TIYVPKGRYYLSTTTFNGPCSNNDITMHVDGTLVAPSDYGV----ENWITFRHVDGVSVF 120

Query: 121 GGVLDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVK 180
           GGVLD QG GLW CKNS  SCP GATSLEFSNSKNIIVSGLTSLNSQMFHIV N CQNVK
Sbjct: 121 GGVLDGQGAGLWDCKNSHKSCPLGATSLEFSNSKNIIVSGLTSLNSQMFHIVANGCQNVK 180

Query: 181 MQGLKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGH 240
           MQG+K+SAAGNSPNTDGIHVA SS VTI++S I TGDDCIS+GP TSNLWIENVACGPGH
Sbjct: 181 MQGIKISAAGNSPNTDGIHVALSSQVTIISSSISTGDDCISVGPGTSNLWIENVACGPGH 240

Query: 241 GISIGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVE 300
           GISIGSLG++++E+GVQNVTVK+ TFTNTQNGVRIKTWG+PSKGFA+NI+F+DI+M NV+
Sbjct: 241 GISIGSLGKDVEEDGVQNVTVKTTTFTNTQNGVRIKTWGKPSKGFARNILFQDIIMNNVQ 300

Query: 301 NPIIIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLED 360
           NPIIIDQNYCP NKGCPGQ SGV+IS+V Y++IHGTSATQVAMKFDCSSKFPC  ITLED
Sbjct: 301 NPIIIDQNYCPNNKGCPGQVSGVEISNVSYQNIHGTSATQVAMKFDCSSKFPCRGITLED 360

Query: 361 VKLSYKNEAAKASCSHAEGSVASLVQPSSCL 390
           VKLSYKN+ A+ASCS+A+G+ A LVQPSSCL
Sbjct: 361 VKLSYKNDVAEASCSNADGTAAGLVQPSSCL 387

BLAST of Lsi05G008450 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 503.1 bits (1294), Expect = 2.1e-142
Identity = 243/388 (62.63%), Postives = 297/388 (76.55%), Query Frame = 0

Query: 3   LHTTSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYV 62
           +H++  I+L  L   SS+S  T+N++ +GAKPD KTDS+KAF   WA AC +    +I V
Sbjct: 1   MHSSLLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILV 60

Query: 63  PKGKFYLSSTTFDG-PCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGV 122
           PKG+F L S  FDG  C    +T RI GTL APS+YRVI     WI F+H+DG+ V+GGV
Sbjct: 61  PKGRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGV 120

Query: 123 LDAQGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQG 182
           LDAQG  LW+CK S  +CPSGATS+ F +S+N+++SGLTSLNSQMFH+ IN C NVK+ G
Sbjct: 121 LDAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDG 180

Query: 183 LKVSAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGIS 242
           +KVSA GNSPNTDGIHV  SS V+ILNS I TGDDC+SIGP T+ LWIENVACGPGHGIS
Sbjct: 181 VKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGIS 240

Query: 243 IGSLGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPI 302
           IGSLG+E  E GVQN+TVK+ATFT T+NGVRIK+W RPS GFAKNI F+  VM NV+NPI
Sbjct: 241 IGSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPI 300

Query: 303 IIDQNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKL 362
           +IDQNYCP N+ CP Q SG+KISDV +  IHGTSAT+V +K DCSSK PC+ I ++DVKL
Sbjct: 301 VIDQNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKL 360

Query: 363 SYKNEAAKASCSHAEGSVASLVQPSSCL 390
           +Y+N+ A   CSHA GS A   +P+SCL
Sbjct: 361 TYRNKPATTDCSHAGGSEAGFQRPNSCL 388

BLAST of Lsi05G008450 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 494.6 bits (1272), Expect = 7.4e-140
Identity = 244/384 (63.54%), Postives = 299/384 (77.86%), Query Frame = 0

Query: 7   SFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKGK 66
           S ++L + F+ SS S  ++NV+ FGAKPD KTD++KAF A W  AC +S   +I VPKG+
Sbjct: 3   SLLVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGR 62

Query: 67  FYLSSTTFDG-PCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVLDAQ 126
           F L S TFDG  C    +T RIDGTL AP++YRVI N + WI F+H+DG+ V+GGVLDA+
Sbjct: 63  FLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDAR 122

Query: 127 GFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVS 186
           G  LW CK S  +CPSGAT++ F +S N++VSGLTSLNSQMFH+VIN C NVK+QG+KV 
Sbjct: 123 GASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVL 182

Query: 187 AAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSL 246
           AAGNSPNTDGIHV  SS+V+I N+ I TGDDC+SIGP T+ LWIENVACGPGHGISIGSL
Sbjct: 183 AAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 242

Query: 247 GREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQ 306
           G++  E+GVQNVTVK+ TFT T NGVRIK+W RPS GFAKNI F+  VM NVENPIIIDQ
Sbjct: 243 GKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQ 302

Query: 307 NYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYKN 366
           NYCP++  CP Q SG+KISDV +  IHGTSAT+V +K DCSSK PC+ I LEDVKL+Y+N
Sbjct: 303 NYCPDH-DCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQN 362

Query: 367 EAAKASCSHAEGSVASLVQPSSCL 390
           + A ++C+HA G  A   QP +CL
Sbjct: 363 KPAASACTHAGGIEAGFFQP-NCL 384

BLAST of Lsi05G008450 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 413.7 bits (1062), Expect = 1.7e-115
Identity = 202/386 (52.33%), Postives = 269/386 (69.69%), Query Frame = 0

Query: 8   FILLHLLFYFSSASLS---TFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPK 67
           F LL  L  F   S+S    FNVV FGAKPD  TDS+ AF  AW GAC ++++ ++ VPK
Sbjct: 8   FSLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPK 67

Query: 68  GKFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVLDA 127
           G F L   TF GPC +  IT ++ GT+ AP +YR   NS  WI F  V+   + GG  DA
Sbjct: 68  GTFLLKVITFGGPCKSK-ITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDA 127

Query: 128 QGFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKV 187
           +  G W+C+ S  +CP G  S+ F+++K++I+SG+ S+NSQ+ H+ +N C NV ++ +K+
Sbjct: 128 RANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKL 187

Query: 188 SAAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGS 247
            A GNSPNTDG HV  S+ VT   S + TGDDC++IGP T NL I  +ACGPGHG+SIGS
Sbjct: 188 VAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGS 247

Query: 248 LGREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIID 307
           L +E++E+GV+NVTV S+ FT +QNGVRIK+W RPS GF + + F+D+VM NVENPIIID
Sbjct: 248 LAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIID 307

Query: 308 QNYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSY- 367
           QNYCP ++GCP + SGVKIS V Y++I GTSATQ AMK  CS   PC+ ITL+D+KL+Y 
Sbjct: 308 QNYCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYN 367

Query: 368 KNEAAKASCSHAEGSVASLVQPSSCL 390
           K   A + C +A G    ++QP+SCL
Sbjct: 368 KGTPATSFCFNAVGKSLGVIQPTSCL 392

BLAST of Lsi05G008450 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 4.1e-114
Identity = 197/384 (51.30%), Postives = 267/384 (69.53%), Query Frame = 0

Query: 6   TSFILLHLLFYFSSASLSTFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKG 65
           +SF+L+      SS + S +NVV FGAKPD +TDS+KAF  AW  ACR++   ++ VP+G
Sbjct: 15  SSFLLMK-----SSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVTVPRG 74

Query: 66  KFYLSSTTFDGPCNNNDITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVLDAQ 125
            F L    F GPC +  IT +I GT+ APS+YR + NS  WI F  V+ + + GG LDA+
Sbjct: 75  SFLLKPVEFRGPCRSR-ITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGTLDAR 134

Query: 126 GFGLWACKNSKNSCPSGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVS 185
           G   WAC+ S  SCP GA S+ F+ + +++VSGLTS+NSQ  H+VIN C NV ++ +K+ 
Sbjct: 135 GASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRKVKLV 194

Query: 186 AAGNSPNTDGIHVAQSSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSL 245
           A   SPNTDG+HV  S+ VT+ +    TGDDCISIGP T NL++  + CGPGHGISIGSL
Sbjct: 195 APDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGISIGSL 254

Query: 246 GREMQENGVQNVTVKSATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQ 305
           GR+  E GV+N+T+ ++ F+ + NGVRIKTW R S GF +N++F++++M NV+NPII+DQ
Sbjct: 255 GRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPIIVDQ 314

Query: 306 NYCPENKGCPGQDSGVKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYKN 365
           NYCP N+GCP Q SGVKIS V YR+I GTS TQ A+ FDCS   PC  I L D+KL++  
Sbjct: 315 NYCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFNG 374

Query: 366 EAAKASCSHAEGSVASLVQPSSCL 390
            +A ++C + +G  A +V P  CL
Sbjct: 375 RSATSTCKNIKGVKAGVVMPQGCL 392

BLAST of Lsi05G008450 vs. TAIR 10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.2e-112
Identity = 204/369 (55.28%), Postives = 266/369 (72.09%), Query Frame = 0

Query: 23  STFNVVDFGAKPDTKTDSSKAFEAAWAGACRASTTGSIYVPKGKFYLSSTTFDG-PCNNN 82
           ST NV+ +GAKPD   DS+KAF AAW  AC ++   +I VPKG+F + +  F G  C   
Sbjct: 31  STLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQA 90

Query: 83  DITIRIDGTLFAPSNYRVIANSENWITFRHVDGVGVFGGVLDAQGFGLWACKNS-KNSCP 142
            I+IRI G++ AP ++R+IA+S++WI F  V  V ++GG+LDAQG  LW CKN+  ++CP
Sbjct: 91  PISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCP 150

Query: 143 SGATSLEFSNSKNIIVSGLTSLNSQMFHIVINDCQNVKMQGLKVSAAGNSPNTDGIHVAQ 202
           +GA SL FS S NI +SGLTS+NSQ FHIVI++  NV + G+KVSA  NSPNTDGIHV  
Sbjct: 151 TGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVES 210

Query: 203 SSAVTILNSIIGTGDDCISIGPDTSNLWIENVACGPGHGISIGSLGREMQENGVQNVTVK 262
           S +V I NS IGTGDDCISIGP ++N++I+ + CGPGHGISIGSLGR  +E GV NVTV 
Sbjct: 211 SHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVS 270

Query: 263 SATFTNTQNGVRIKTWGRPSKGFAKNIIFKDIVMVNVENPIIIDQNYCPENKGCPGQDSG 322
           +  F  T NGVRIKTWG+ S  FA+NI+F+ I M  V+NPIIIDQ+YC  +K CP Q+SG
Sbjct: 271 NVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYC-LHKPCPKQESG 330

Query: 323 VKISDVKYRSIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSYKNEAAKASCSHAEGSVA 382
           VK+S+V+Y  IHGTS T+VA+  DCS + PC+ I ++DV L   +  A+ASC +A GS  
Sbjct: 331 VKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSAN 390

Query: 383 SLVQPSSCL 390
            +V  + CL
Sbjct: 391 DVVPFTPCL 398

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489791.3e-14967.78Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228183.1e-11155.28Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
Q6H9K03.0e-7742.20Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
P353367.3e-7644.32Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q9SFB73.6e-7543.54Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3DV941.1e-18884.83Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A5A7ULI93.3e-18884.83Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3... [more]
A0A0A0M0955.3e-18684.58Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627460 PE=3 SV=1[more]
A0A6J1EYE02.7e-17477.75polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439559 PE=3 SV=1[more]
A0A6J1DRR73.7e-16373.59polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023699 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888709.13.7e-19487.18polygalacturonase-like [Benincasa hispida][more]
TYK27707.12.3e-18884.83polygalacturonase-like [Cucumis melo var. makuwa][more]
KAA0056020.16.8e-18884.83polygalacturonase-like [Cucumis melo var. makuwa][more]
XP_004139060.11.1e-18584.58polygalacturonase [Cucumis sativus] >KGN66537.1 hypothetical protein Csa_007593 ... [more]
XP_022932944.15.6e-17477.75polygalacturonase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G59850.12.1e-14262.63Pectin lyase-like superfamily protein [more]
AT2G43870.17.4e-14063.54Pectin lyase-like superfamily protein [more]
AT1G05660.11.7e-11552.33Pectin lyase-like superfamily protein [more]
AT2G43890.14.1e-11451.30Pectin lyase-like superfamily protein [more]
AT2G43860.12.2e-11255.28Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 201..222
e-value: 550.0
score: 9.1
coord: 174..200
e-value: 420.0
score: 9.7
coord: 284..305
e-value: 4600.0
score: 2.5
coord: 254..275
e-value: 16.0
score: 14.4
coord: 224..244
e-value: 2200.0
score: 4.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 52..375
e-value: 4.1E-89
score: 298.9
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 231..244
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 16..388
e-value: 2.5E-118
score: 397.6
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 8..388
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 8..388
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 9..389

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G008450.1Lsi05G008450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity