Lsi05G007420 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G007420 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.7e-52
Identity = 136/345 (39.42%), Postives = 195/345 (56.52%), Query Frame = 0

Query: 43  LPPPSQNQTNSLAKFVSTAAG-LFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLV 102
           LP   +       +FV  A    FP++++ G ++  ++PSTF+W     P    + L + 
Sbjct: 78  LPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGLTIT 137

Query: 103 MLAMGLTLQLKDL-------FNLFMQRPLSYTIMPVVGALIGKFLGLPPSLSVGLILLGC 162
           ML MG+TL L DL         LF    L Y++MP+    + K L LPP  + GLIL+GC
Sbjct: 138 MLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLILVGC 197

Query: 163 CPGGIASSVVTLIARGDVPLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTL 222
           CPGG AS++VT IARG+V LS++MT  +T+ AVI+TP LT  L   +I VDA  L +STL
Sbjct: 198 CPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLLMSTL 257

Query: 223 QVVVVPILVGSYLQKACPELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTL 282
           QVV++P+L G++L +   +LVK + P  P  AV T ++L      +N   +         
Sbjct: 258 QVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAI--------- 317

Query: 283 SPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQ 342
                       ++SG+   V+LA  LLH++GF  GY+ + I G      R IS+EVGMQ
Sbjct: 318 ------------LMSGK--QVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQ 377

Query: 343 NSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNFEP 380
           NS LGVVLAT HF + + A+P A+S+V  +++GS L   WR   P
Sbjct: 378 NSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSAP 396

BLAST of Lsi05G007420 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.7e-52
Identity = 130/343 (37.90%), Postives = 195/343 (56.85%), Query Frame = 0

Query: 42  SLPPPSQNQTNSLAKFVSTAAGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLV 101
           +LP    ++ N   K V     LFP+++  G I+   KPS  +W        +++ LG +
Sbjct: 91  NLPESIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSMVTWL---ETDLFTVGLGFL 150

Query: 102 MLAMGLTLQLKDLFNLFMQRP--------LSYTIMPVVGALIGKFLGLPPSLSVGLILLG 161
           ML+MGLTL  +D F   M+ P          Y I P++G  I   L L   L+ GLIL+ 
Sbjct: 151 MLSMGLTLTFED-FRRCMRNPWTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLATGLILVS 210

Query: 162 CCPGGIASSVVTLIARGDVPLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLST 221
           CCPGG AS+V T I++G+V LS++MT C+T+ A+++TP LTK L G  +PVDAA L++ST
Sbjct: 211 CCPGGQASNVATYISKGNVALSVLMTTCSTIGAIVMTPLLTKLLAGQLVPVDAAGLAIST 270

Query: 222 LQVVVVPILVGSYLQKACPELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSST 281
            QVV++P +VG    +  P+  +RII  +PL  VL ++LL  S   +             
Sbjct: 271 FQVVLLPTIVGVLAHEYFPKFTERIISITPLIGVLLTTLLCASPIGQ------------- 330

Query: 282 LSPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGM 341
                     +  +L  + G +I+ V LLH+  F +GY ++ +  F E   R IS+E GM
Sbjct: 331 ----------VSEVLKAQGGQLIIPVALLHVAAFALGYWLSKVSSFGESTSRTISIECGM 390

Query: 342 QNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRN 377
           Q+S+LG +LA  HF++ +VA+P+A+S V M + GS+L  FWRN
Sbjct: 391 QSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSALAVFWRN 406

BLAST of Lsi05G007420 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.1e-50
Identity = 136/350 (38.86%), Postives = 195/350 (55.71%), Query Frame = 0

Query: 43  LPPPSQNQTNSLAKFVSTAAGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVM 102
           LP  +  + +   K +     LFPL++  G +V   KPS  +W        ++L LG +M
Sbjct: 83  LPESTPKELSQYEKIIELLTTLFPLWVILGTLVGIFKPSLVTWL---ETDLFTLGLGFLM 142

Query: 103 LAMGLTLQLKDLFNLFMQRP--------LSYTIMPVVGALIGKFLGLPPSLSVGLILLGC 162
           L+MGLTL  +D F   ++ P          Y I P++G LI   L L   L+ GLIL+ C
Sbjct: 143 LSMGLTLTFED-FRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSC 202

Query: 163 CPGGIASSVVTLIARGDVPLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTL 222
           CPGG AS+V T I++G+V LS++MT C+T+ A+I+TP LTK L G  +PVDAA L+LST 
Sbjct: 203 CPGGQASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLALSTF 262

Query: 223 QVVVVPILVGSYLQKACPELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTL 282
           QVV+VP ++G    +  P+   +II  +PL  V+ ++LL  S   +         V+  L
Sbjct: 263 QVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPIGQ---------VADVL 322

Query: 283 SPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQ 342
               +QL              IL V LLH   F +GY I+    F E   R IS+E GMQ
Sbjct: 323 KTQGAQL--------------ILPVALLHAAAFAIGYWISKF-SFGESTSRTISIECGMQ 382

Query: 343 NSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNFE-PSDSQ 384
           +S+LG +LA  HF++ +VA+P+A+S V M + GS L  FWRN   P+D +
Sbjct: 383 SSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFWRNLPIPADDK 404

BLAST of Lsi05G007420 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 2.9e-48
Identity = 126/324 (38.89%), Postives = 184/324 (56.79%), Query Frame = 0

Query: 65  FPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFN-LFMQRPL 124
           FP+++     VA  +P  F W     P +  + +   ML MG+TL L DL   L M + L
Sbjct: 106 FPVWVASACAVALWRPPAFLWV---SPMAQIVGISFTMLGMGMTLTLDDLKTALLMPKEL 165

Query: 125 S------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLSII 184
           +      Y++MP+ G LI K L LP   + GLIL+ CCPGG AS++VT +ARG+V LS++
Sbjct: 166 ASGFLLQYSVMPLSGFLISKLLNLPSYYAAGLILVSCCPGGTASNIVTYLARGNVALSVL 225

Query: 185 MTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELVKR 244
           MT  +T  A  LTP LT  L G ++ VD   L +ST QVV+ P+L+G+ L + C  LV+ 
Sbjct: 226 MTAASTFAAAFLTPLLTSKLAGQYVAVDPMGLFVSTSQVVLAPVLLGALLNQYCNGLVQL 285

Query: 245 IIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVVIL 304
           + P  P  AV T ++L  +  ++N     S+++SS L                    V++
Sbjct: 286 VSPLMPFIAVATVAVLCGNAIAQN----ASAILSSGLQ-------------------VVM 345

Query: 305 AVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA 364
           +V  LH +GFF GY+++   G      R IS+EVGMQNS LGVVLA+ HF + + A+P A
Sbjct: 346 SVCWLHASGFFFGYVLSRTIGIDISSSRTISIEVGMQNSVLGVVLASKHFGNPLTAVPCA 403

Query: 365 MSAVIMNMMGSSLGSFWRNFEPSD 382
           +S+V  ++ GS L   WR+  P+D
Sbjct: 406 VSSVCHSVYGSLLAGIWRSLPPND 403

BLAST of Lsi05G007420 vs. ExPASy Swiss-Prot
Match: Q6K739 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.7e-36
Identity = 113/325 (34.77%), Postives = 175/325 (53.85%), Query Frame = 0

Query: 62  AGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQR 121
           + L PL +    + A   P+TFSW  +     Y+ ALG +ML++G+ L + D F L  +R
Sbjct: 105 SALLPLVVAATAVAALGNPATFSWVSKE---YYAPALGGIMLSIGIKLSIDD-FALAFKR 164

Query: 122 PL--------SYTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVP 181
           P+         Y + P++G LI +  G+P +   G +L  C  G   SS  + +++GDV 
Sbjct: 165 PVPLTIGYMAQYIVKPLMGVLIARAFGMPSAFFAGFVLTCCVSGAQLSSYASFLSKGDVA 224

Query: 182 LSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPE 241
           LSI++T C+T+ +V++TP LT  L+G+ +PVD   ++ S LQVV+VP+ +G  L      
Sbjct: 225 LSILLTSCSTISSVVVTPVLTGLLIGSVVPVDGIAMAKSILQVVLVPVTLGLLLNTYAKA 284

Query: 242 LVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELG 301
           +V  I P  P  A+L +SL                 + S L+ N S+      ILS E  
Sbjct: 285 VVNVIQPVMPFVAMLCTSL----------------CIGSPLAINRSK------ILSSEGF 344

Query: 302 VVILAVFLLHLTGFFVGYIIAAIGGFRERER--RAISLEVGMQNSSLGVVLATAHFSSAM 361
           +++L +   H+  F VGY I+ +   R+ E   R IS+  GMQ+S+L  +LAT    S+ 
Sbjct: 345 LLLLPIVTFHIAAFIVGYWISKLPMLRQEEPVCRTISVCTGMQSSTLAGLLATQFLGSSQ 402

Query: 362 VALPAAMSAVIMNMMGSSLGSFWRN 377
            A+PAA S VIM + G +L S+W N
Sbjct: 405 -AVPAACSVVIMAIFGLTLASYWGN 402

BLAST of Lsi05G007420 vs. ExPASy TrEMBL
Match: A0A0A0L5D6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G106010 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.9e-168
Identity = 329/383 (85.90%), Postives = 348/383 (90.86%), Query Frame = 0

Query: 6   RNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTAAGLF 65
           R++Y+PLRLNPLLLPP KPPG+L IVRSLQRDTEL  LPPP Q QT+ LAKFVSTAAGLF
Sbjct: 20  RHFYKPLRLNPLLLPPPKPPGYLAIVRSLQRDTELLPLPPPPQKQTDRLAKFVSTAAGLF 79

Query: 66  PLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPLS- 125
           PLYIT GGIVAC+KPSTFSWFVQRGPGSYSLAL LVMLAMGLTL+LKDLFNLFMQRPLS 
Sbjct: 80  PLYITSGGIVACMKPSTFSWFVQRGPGSYSLALSLVMLAMGLTLELKDLFNLFMQRPLSI 139

Query: 126 -------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLSII 185
                  YTIMPVVGA IGKFLGL P+LSVGLILLGCCPGGIASSVVTLIARGDVPLSII
Sbjct: 140 LYLCVAQYTIMPVVGAAIGKFLGLAPALSVGLILLGCCPGGIASSVVTLIARGDVPLSII 199

Query: 186 MTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELVKR 245
           MTVCTTL+AV+LTPFLT+TLVGA IPVDA KLSLSTLQVVVVPIL+GSYLQK CP +VKR
Sbjct: 200 MTVCTTLQAVVLTPFLTRTLVGASIPVDAIKLSLSTLQVVVVPILIGSYLQKTCPGVVKR 259

Query: 246 IIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVVIL 305
           IIPFSPLFAVLTSSLLACSVFSENI+R KSSLVSS+LS + S  I +KT+LS ELG+VIL
Sbjct: 260 IIPFSPLFAVLTSSLLACSVFSENIVRFKSSLVSSSLSFDASPWIAIKTMLSEELGIVIL 319

Query: 306 AVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA 365
           AVF LHL GF VGY IAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA
Sbjct: 320 AVFSLHLAGFVVGYTIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA 379

Query: 366 MSAVIMNMMGSSLGSFWRNFEPS 381
           MSAVIMNMMGSSLGSFWRN EPS
Sbjct: 380 MSAVIMNMMGSSLGSFWRNIEPS 402

BLAST of Lsi05G007420 vs. ExPASy TrEMBL
Match: A0A1S3BUJ9 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493326 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.7e-166
Identity = 326/388 (84.02%), Postives = 347/388 (89.43%), Query Frame = 0

Query: 2   SSRHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTA 61
           S   R++Y+PLRLNPL LPP KPPG+L IVRSLQRDTEL  LPPP Q QT  L KFVSTA
Sbjct: 15  SLHQRHFYKPLRLNPLRLPPPKPPGYLAIVRSLQRDTELLPLPPPPQKQTGRLEKFVSTA 74

Query: 62  AGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQR 121
           AGLFPLYIT GGI+AC+KPSTFSWFVQRGPGSYSLAL LVMLAMGLTL+LKDLFNLFMQR
Sbjct: 75  AGLFPLYITSGGIIACMKPSTFSWFVQRGPGSYSLALSLVMLAMGLTLELKDLFNLFMQR 134

Query: 122 PLS--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVP 181
           PL+        YTIMPVVGA+IGKFLGLPP LSVGLILLGCCPGGIASSVVTLIA+GDVP
Sbjct: 135 PLAILYVCVAQYTIMPVVGAVIGKFLGLPPGLSVGLILLGCCPGGIASSVVTLIAKGDVP 194

Query: 182 LSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPE 241
           LSIIMTVCTTL+AV+LTPFLT+TLVGA IPVDA KLSLSTLQVVVVPIL+GSYLQKACP 
Sbjct: 195 LSIIMTVCTTLQAVVLTPFLTRTLVGASIPVDAIKLSLSTLQVVVVPILIGSYLQKACPG 254

Query: 242 LVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSS-LVSSTLSPNTSQLIGLKTILSGEL 301
           +VKRI+PFSPLFAVLTSSLLACSVFSENI+RLKSS LVSS+L  + S  I +KT+LS EL
Sbjct: 255 VVKRIVPFSPLFAVLTSSLLACSVFSENIVRLKSSVLVSSSLGFDASPWIAIKTMLSREL 314

Query: 302 GVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMV 361
           GVVILAVF LHL GF  GY IAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMV
Sbjct: 315 GVVILAVFSLHLAGFIAGYTIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMV 374

Query: 362 ALPAAMSAVIMNMMGSSLGSFWRNFEPS 381
           ALPAAMSAVIMNMMGSSLGSFWRN EPS
Sbjct: 375 ALPAAMSAVIMNMMGSSLGSFWRNIEPS 402

BLAST of Lsi05G007420 vs. ExPASy TrEMBL
Match: A0A5A7V423 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00580 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 4.1e-162
Identity = 327/414 (78.99%), Postives = 347/414 (83.82%), Query Frame = 0

Query: 2   SSRHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTA 61
           S   R++Y+PLRLNPL LPP KPPG+L IVRSLQRDTEL  LPPP Q QT  L KFVSTA
Sbjct: 15  SLHQRHFYKPLRLNPLRLPPPKPPGYLAIVRSLQRDTELLPLPPPPQKQTGRLEKFVSTA 74

Query: 62  AGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQR 121
           AGLFPLYIT GGI+AC+KPSTFSWFVQRGPGSYSLAL LVMLAMGLTL+LKDLFNLFMQR
Sbjct: 75  AGLFPLYITSGGIIACMKPSTFSWFVQRGPGSYSLALSLVMLAMGLTLELKDLFNLFMQR 134

Query: 122 PLS--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVP 181
           PL+        YTIMPVVGA+IGKFLGLPP LSVGLILLGCCPGGIASSVVTLIA+GDVP
Sbjct: 135 PLAILYVCVAQYTIMPVVGAVIGKFLGLPPGLSVGLILLGCCPGGIASSVVTLIAKGDVP 194

Query: 182 LSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPE 241
           LSIIMTVCTTLEAV+LTPFLT+TLVGA IPVDA KLSLSTLQVVVVPIL+GSYLQKACP 
Sbjct: 195 LSIIMTVCTTLEAVVLTPFLTRTLVGASIPVDAIKLSLSTLQVVVVPILIGSYLQKACPG 254

Query: 242 LVKRIIPFSPLFAVLTSSLLAC--------------------------SVFSENIIRLKS 301
           +VKRI+PFSPLFAVLTSSLLAC                          SVFSENI+RLKS
Sbjct: 255 VVKRIVPFSPLFAVLTSSLLACRFSFSLYFNFLIVCALIFSLILVSFISVFSENIVRLKS 314

Query: 302 S-LVSSTLSPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRA 361
           S LVSS+L  + S  I +KT+LS ELGVVILAVF LHL GF  GY IAAIGGFRERERRA
Sbjct: 315 SVLVSSSLGFDASPWIAIKTMLSRELGVVILAVFSLHLAGFIAGYTIAAIGGFRERERRA 374

Query: 362 ISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNFEPS 381
           ISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRN EPS
Sbjct: 375 ISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNIEPS 428

BLAST of Lsi05G007420 vs. ExPASy TrEMBL
Match: A0A6J1JB28 (sodium/pyruvate cotransporter BASS2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111483378 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.8e-155
Identity = 313/390 (80.26%), Postives = 336/390 (86.15%), Query Frame = 0

Query: 4   RHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTAAG 63
           R   +YRPL     L  PSK PGHL  VRSLQR+TELPSLP P Q  T  + KFVSTAAG
Sbjct: 20  RKLRHYRPL---TPLFRPSKHPGHLA-VRSLQRNTELPSLPTPPQKPT-PVVKFVSTAAG 79

Query: 64  LFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL 123
           LFPLYIT GG+VAC+KPSTFSWFV+RGPGSYSLALGLVMLAMGLTL+LKDLF LFMQRPL
Sbjct: 80  LFPLYITAGGVVACLKPSTFSWFVRRGPGSYSLALGLVMLAMGLTLELKDLFTLFMQRPL 139

Query: 124 S--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLS 183
           S        +TIMPVVG LIGK+LGLPP LSVGL+LLGCCPGGIASSVVTLIARGDVPLS
Sbjct: 140 SILYVCIAQFTIMPVVGVLIGKYLGLPPPLSVGLVLLGCCPGGIASSVVTLIARGDVPLS 199

Query: 184 IIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELV 243
           IIMTVCTTL AVILTPFLTKTLVGAF+PVDA KLSLSTLQVVV PIL+GSYLQKACP+LV
Sbjct: 200 IIMTVCTTLGAVILTPFLTKTLVGAFVPVDALKLSLSTLQVVVAPILLGSYLQKACPKLV 259

Query: 244 KRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVV 303
           KRI+ FSPL AVLTSSLLACSVFSEN +R KSSLVSSTL  + S  IG+KTILSGELG +
Sbjct: 260 KRIVTFSPLVAVLTSSLLACSVFSENFVRFKSSLVSSTLGSDVSSWIGVKTILSGELGTI 319

Query: 304 ILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP 363
           IL+VFLLHL GFFVGY IA IGGF+ERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP
Sbjct: 320 ILSVFLLHLVGFFVGYTIAVIGGFQERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP 379

Query: 364 AAMSAVIMNMMGSSLGSFWRNFEPSDSQTA 386
            AMSAVIMNMMGSSLGS WRN  P+ ++ A
Sbjct: 380 PAMSAVIMNMMGSSLGSLWRNIPPTVAEAA 404

BLAST of Lsi05G007420 vs. ExPASy TrEMBL
Match: A0A6J1ERH2 (sodium/pyruvate cotransporter BASS2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111435881 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.4e-154
Identity = 314/390 (80.51%), Postives = 337/390 (86.41%), Query Frame = 0

Query: 4   RHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTAAG 63
           R   +YRP  L PLL  P KPPGHL  VRSLQR+TELPSLP P Q  T  +AKFVSTAAG
Sbjct: 20  RKLRHYRP--LTPLLRQP-KPPGHLA-VRSLQRNTELPSLPTPPQKPT-PVAKFVSTAAG 79

Query: 64  LFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL 123
           LFPLYIT GG+VAC+KPSTFSWFV+RGPGSYSLALGLVMLAMGLTL+LKDLF LFMQRPL
Sbjct: 80  LFPLYITAGGVVACLKPSTFSWFVRRGPGSYSLALGLVMLAMGLTLELKDLFTLFMQRPL 139

Query: 124 S--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLS 183
           S        +T+MPVVGALIGK+LGLPP LSVGL+LLGCCPGGIASSVVTLIARGDVPLS
Sbjct: 140 SILYVCIAQFTVMPVVGALIGKYLGLPPPLSVGLVLLGCCPGGIASSVVTLIARGDVPLS 199

Query: 184 IIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELV 243
           IIMTVCTTL AVILTPFLTKTLVGAF+PVDA KLSLSTLQVVV PIL+GSYLQKA P+LV
Sbjct: 200 IIMTVCTTLGAVILTPFLTKTLVGAFVPVDALKLSLSTLQVVVAPILLGSYLQKAYPKLV 259

Query: 244 KRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVV 303
           KRI+ FSPL AVLTSSLLACSVFSEN  R KSSLV S L  + S  +G+KTILSGELG V
Sbjct: 260 KRIVTFSPLVAVLTSSLLACSVFSENFARFKSSLVGSALGSDVSTWVGVKTILSGELGAV 319

Query: 304 ILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP 363
           IL+VFLLHL GFFVGY IAAIGGF+ERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP
Sbjct: 320 ILSVFLLHLVGFFVGYTIAAIGGFQERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP 379

Query: 364 AAMSAVIMNMMGSSLGSFWRNFEPSDSQTA 386
            AMSAVIMNMMGSSLGS WRN  P+ ++ A
Sbjct: 380 PAMSAVIMNMMGSSLGSLWRNIPPTVAEAA 404

BLAST of Lsi05G007420 vs. NCBI nr
Match: XP_038905499.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 649.4 bits (1674), Expect = 1.9e-182
Identity = 354/388 (91.24%), Postives = 363/388 (93.56%), Query Frame = 0

Query: 4   RHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTAAG 63
           + R+YY PLRLN   LPP KPPG+L  VRSLQRDTELPSLP P Q +T+ L KFVSTAAG
Sbjct: 17  QQRHYYPPLRLNLRRLPPPKPPGYLATVRSLQRDTELPSLPRPPQKRTSRLTKFVSTAAG 76

Query: 64  LFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL 123
           LFPLYITGGGIVAC+KPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL
Sbjct: 77  LFPLYITGGGIVACLKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL 136

Query: 124 SYTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLSIIMTVCTT 183
           SY IMPVVGALIGKFLGLPP LSVGLILLGCCPGGIASSVVTLIARGDVPLSIIMTVCTT
Sbjct: 137 SYAIMPVVGALIGKFLGLPPPLSVGLILLGCCPGGIASSVVTLIARGDVPLSIIMTVCTT 196

Query: 184 LEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELVKRIIPFSP 243
           LEAV LTPFLTKTLVGAFIPVDA KLSLSTLQVVVVPILVGSYLQKACPELVKRIIPFSP
Sbjct: 197 LEAVFLTPFLTKTLVGAFIPVDAVKLSLSTLQVVVVPILVGSYLQKACPELVKRIIPFSP 256

Query: 244 LFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVVILAVFLLH 303
           LFAVLTSSLLACSVFSENI+RLKSSLVSS LS N S  IG KT+LSGELGVVILAVFLLH
Sbjct: 257 LFAVLTSSLLACSVFSENIVRLKSSLVSSALSSNASPWIGFKTMLSGELGVVILAVFLLH 316

Query: 304 LTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIM 363
           LTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIM
Sbjct: 317 LTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIM 376

Query: 364 NMMGSSLGSFWRNFEPSDSQTATLTSTA 392
           NMMGSSLGSFWRNF+PSDSQT TLTS A
Sbjct: 377 NMMGSSLGSFWRNFQPSDSQTPTLTSPA 404

BLAST of Lsi05G007420 vs. NCBI nr
Match: XP_038905498.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 642.1 bits (1655), Expect = 3.1e-180
Identity = 354/396 (89.39%), Postives = 363/396 (91.67%), Query Frame = 0

Query: 4   RHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTAAG 63
           + R+YY PLRLN   LPP KPPG+L  VRSLQRDTELPSLP P Q +T+ L KFVSTAAG
Sbjct: 17  QQRHYYPPLRLNLRRLPPPKPPGYLATVRSLQRDTELPSLPRPPQKRTSRLTKFVSTAAG 76

Query: 64  LFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL 123
           LFPLYITGGGIVAC+KPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL
Sbjct: 77  LFPLYITGGGIVACLKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPL 136

Query: 124 S--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLS 183
           S        Y IMPVVGALIGKFLGLPP LSVGLILLGCCPGGIASSVVTLIARGDVPLS
Sbjct: 137 SILYVCVAQYAIMPVVGALIGKFLGLPPPLSVGLILLGCCPGGIASSVVTLIARGDVPLS 196

Query: 184 IIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELV 243
           IIMTVCTTLEAV LTPFLTKTLVGAFIPVDA KLSLSTLQVVVVPILVGSYLQKACPELV
Sbjct: 197 IIMTVCTTLEAVFLTPFLTKTLVGAFIPVDAVKLSLSTLQVVVVPILVGSYLQKACPELV 256

Query: 244 KRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVV 303
           KRIIPFSPLFAVLTSSLLACSVFSENI+RLKSSLVSS LS N S  IG KT+LSGELGVV
Sbjct: 257 KRIIPFSPLFAVLTSSLLACSVFSENIVRLKSSLVSSALSSNASPWIGFKTMLSGELGVV 316

Query: 304 ILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP 363
           ILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP
Sbjct: 317 ILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALP 376

Query: 364 AAMSAVIMNMMGSSLGSFWRNFEPSDSQTATLTSTA 392
           AAMSAVIMNMMGSSLGSFWRNF+PSDSQT TLTS A
Sbjct: 377 AAMSAVIMNMMGSSLGSFWRNFQPSDSQTPTLTSPA 412

BLAST of Lsi05G007420 vs. NCBI nr
Match: XP_011650565.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucumis sativus] >KGN56249.1 hypothetical protein Csa_009901 [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 6.0e-168
Identity = 329/383 (85.90%), Postives = 348/383 (90.86%), Query Frame = 0

Query: 6   RNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTAAGLF 65
           R++Y+PLRLNPLLLPP KPPG+L IVRSLQRDTEL  LPPP Q QT+ LAKFVSTAAGLF
Sbjct: 20  RHFYKPLRLNPLLLPPPKPPGYLAIVRSLQRDTELLPLPPPPQKQTDRLAKFVSTAAGLF 79

Query: 66  PLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPLS- 125
           PLYIT GGIVAC+KPSTFSWFVQRGPGSYSLAL LVMLAMGLTL+LKDLFNLFMQRPLS 
Sbjct: 80  PLYITSGGIVACMKPSTFSWFVQRGPGSYSLALSLVMLAMGLTLELKDLFNLFMQRPLSI 139

Query: 126 -------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVPLSII 185
                  YTIMPVVGA IGKFLGL P+LSVGLILLGCCPGGIASSVVTLIARGDVPLSII
Sbjct: 140 LYLCVAQYTIMPVVGAAIGKFLGLAPALSVGLILLGCCPGGIASSVVTLIARGDVPLSII 199

Query: 186 MTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPELVKR 245
           MTVCTTL+AV+LTPFLT+TLVGA IPVDA KLSLSTLQVVVVPIL+GSYLQK CP +VKR
Sbjct: 200 MTVCTTLQAVVLTPFLTRTLVGASIPVDAIKLSLSTLQVVVVPILIGSYLQKTCPGVVKR 259

Query: 246 IIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELGVVIL 305
           IIPFSPLFAVLTSSLLACSVFSENI+R KSSLVSS+LS + S  I +KT+LS ELG+VIL
Sbjct: 260 IIPFSPLFAVLTSSLLACSVFSENIVRFKSSLVSSSLSFDASPWIAIKTMLSEELGIVIL 319

Query: 306 AVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA 365
           AVF LHL GF VGY IAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA
Sbjct: 320 AVFSLHLAGFVVGYTIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMVALPAA 379

Query: 366 MSAVIMNMMGSSLGSFWRNFEPS 381
           MSAVIMNMMGSSLGSFWRN EPS
Sbjct: 380 MSAVIMNMMGSSLGSFWRNIEPS 402

BLAST of Lsi05G007420 vs. NCBI nr
Match: XP_008452254.1 (PREDICTED: probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucumis melo])

HSP 1 Score: 594.0 bits (1530), Expect = 9.6e-166
Identity = 326/388 (84.02%), Postives = 347/388 (89.43%), Query Frame = 0

Query: 2   SSRHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTA 61
           S   R++Y+PLRLNPL LPP KPPG+L IVRSLQRDTEL  LPPP Q QT  L KFVSTA
Sbjct: 15  SLHQRHFYKPLRLNPLRLPPPKPPGYLAIVRSLQRDTELLPLPPPPQKQTGRLEKFVSTA 74

Query: 62  AGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQR 121
           AGLFPLYIT GGI+AC+KPSTFSWFVQRGPGSYSLAL LVMLAMGLTL+LKDLFNLFMQR
Sbjct: 75  AGLFPLYITSGGIIACMKPSTFSWFVQRGPGSYSLALSLVMLAMGLTLELKDLFNLFMQR 134

Query: 122 PLS--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVP 181
           PL+        YTIMPVVGA+IGKFLGLPP LSVGLILLGCCPGGIASSVVTLIA+GDVP
Sbjct: 135 PLAILYVCVAQYTIMPVVGAVIGKFLGLPPGLSVGLILLGCCPGGIASSVVTLIAKGDVP 194

Query: 182 LSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPE 241
           LSIIMTVCTTL+AV+LTPFLT+TLVGA IPVDA KLSLSTLQVVVVPIL+GSYLQKACP 
Sbjct: 195 LSIIMTVCTTLQAVVLTPFLTRTLVGASIPVDAIKLSLSTLQVVVVPILIGSYLQKACPG 254

Query: 242 LVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSS-LVSSTLSPNTSQLIGLKTILSGEL 301
           +VKRI+PFSPLFAVLTSSLLACSVFSENI+RLKSS LVSS+L  + S  I +KT+LS EL
Sbjct: 255 VVKRIVPFSPLFAVLTSSLLACSVFSENIVRLKSSVLVSSSLGFDASPWIAIKTMLSREL 314

Query: 302 GVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMV 361
           GVVILAVF LHL GF  GY IAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMV
Sbjct: 315 GVVILAVFSLHLAGFIAGYTIAAIGGFRERERRAISLEVGMQNSSLGVVLATAHFSSAMV 374

Query: 362 ALPAAMSAVIMNMMGSSLGSFWRNFEPS 381
           ALPAAMSAVIMNMMGSSLGSFWRN EPS
Sbjct: 375 ALPAAMSAVIMNMMGSSLGSFWRNIEPS 402

BLAST of Lsi05G007420 vs. NCBI nr
Match: KAA0060615.1 (putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa] >TYK02257.1 putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa])

HSP 1 Score: 580.9 bits (1496), Expect = 8.4e-162
Identity = 327/414 (78.99%), Postives = 347/414 (83.82%), Query Frame = 0

Query: 2   SSRHRNYYRPLRLNPLLLPPSKPPGHLVIVRSLQRDTELPSLPPPSQNQTNSLAKFVSTA 61
           S   R++Y+PLRLNPL LPP KPPG+L IVRSLQRDTEL  LPPP Q QT  L KFVSTA
Sbjct: 15  SLHQRHFYKPLRLNPLRLPPPKPPGYLAIVRSLQRDTELLPLPPPPQKQTGRLEKFVSTA 74

Query: 62  AGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQR 121
           AGLFPLYIT GGI+AC+KPSTFSWFVQRGPGSYSLAL LVMLAMGLTL+LKDLFNLFMQR
Sbjct: 75  AGLFPLYITSGGIIACMKPSTFSWFVQRGPGSYSLALSLVMLAMGLTLELKDLFNLFMQR 134

Query: 122 PLS--------YTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVP 181
           PL+        YTIMPVVGA+IGKFLGLPP LSVGLILLGCCPGGIASSVVTLIA+GDVP
Sbjct: 135 PLAILYVCVAQYTIMPVVGAVIGKFLGLPPGLSVGLILLGCCPGGIASSVVTLIAKGDVP 194

Query: 182 LSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPE 241
           LSIIMTVCTTLEAV+LTPFLT+TLVGA IPVDA KLSLSTLQVVVVPIL+GSYLQKACP 
Sbjct: 195 LSIIMTVCTTLEAVVLTPFLTRTLVGASIPVDAIKLSLSTLQVVVVPILIGSYLQKACPG 254

Query: 242 LVKRIIPFSPLFAVLTSSLLAC--------------------------SVFSENIIRLKS 301
           +VKRI+PFSPLFAVLTSSLLAC                          SVFSENI+RLKS
Sbjct: 255 VVKRIVPFSPLFAVLTSSLLACRFSFSLYFNFLIVCALIFSLILVSFISVFSENIVRLKS 314

Query: 302 S-LVSSTLSPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRA 361
           S LVSS+L  + S  I +KT+LS ELGVVILAVF LHL GF  GY IAAIGGFRERERRA
Sbjct: 315 SVLVSSSLGFDASPWIAIKTMLSRELGVVILAVFSLHLAGFIAGYTIAAIGGFRERERRA 374

Query: 362 ISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNFEPS 381
           ISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRN EPS
Sbjct: 375 ISLEVGMQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNIEPS 428

BLAST of Lsi05G007420 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 205.7 bits (522), Expect = 6.9e-53
Identity = 136/345 (39.42%), Postives = 195/345 (56.52%), Query Frame = 0

Query: 43  LPPPSQNQTNSLAKFVSTAAG-LFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLV 102
           LP   +       +FV  A    FP++++ G ++  ++PSTF+W     P    + L + 
Sbjct: 78  LPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGLTIT 137

Query: 103 MLAMGLTLQLKDL-------FNLFMQRPLSYTIMPVVGALIGKFLGLPPSLSVGLILLGC 162
           ML MG+TL L DL         LF    L Y++MP+    + K L LPP  + GLIL+GC
Sbjct: 138 MLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLILVGC 197

Query: 163 CPGGIASSVVTLIARGDVPLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTL 222
           CPGG AS++VT IARG+V LS++MT  +T+ AVI+TP LT  L   +I VDA  L +STL
Sbjct: 198 CPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLLMSTL 257

Query: 223 QVVVVPILVGSYLQKACPELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTL 282
           QVV++P+L G++L +   +LVK + P  P  AV T ++L      +N   +         
Sbjct: 258 QVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAI--------- 317

Query: 283 SPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQ 342
                       ++SG+   V+LA  LLH++GF  GY+ + I G      R IS+EVGMQ
Sbjct: 318 ------------LMSGK--QVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQ 377

Query: 343 NSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNFEP 380
           NS LGVVLAT HF + + A+P A+S+V  +++GS L   WR   P
Sbjct: 378 NSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSAP 396

BLAST of Lsi05G007420 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 200.7 bits (509), Expect = 2.2e-51
Identity = 136/350 (38.86%), Postives = 195/350 (55.71%), Query Frame = 0

Query: 43  LPPPSQNQTNSLAKFVSTAAGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVM 102
           LP  +  + +   K +     LFPL++  G +V   KPS  +W        ++L LG +M
Sbjct: 83  LPESTPKELSQYEKIIELLTTLFPLWVILGTLVGIFKPSLVTWL---ETDLFTLGLGFLM 142

Query: 103 LAMGLTLQLKDLFNLFMQRP--------LSYTIMPVVGALIGKFLGLPPSLSVGLILLGC 162
           L+MGLTL  +D F   ++ P          Y I P++G LI   L L   L+ GLIL+ C
Sbjct: 143 LSMGLTLTFED-FRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSC 202

Query: 163 CPGGIASSVVTLIARGDVPLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTL 222
           CPGG AS+V T I++G+V LS++MT C+T+ A+I+TP LTK L G  +PVDAA L+LST 
Sbjct: 203 CPGGQASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLALSTF 262

Query: 223 QVVVVPILVGSYLQKACPELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTL 282
           QVV+VP ++G    +  P+   +II  +PL  V+ ++LL  S   +         V+  L
Sbjct: 263 QVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPIGQ---------VADVL 322

Query: 283 SPNTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERERRAISLEVGMQ 342
               +QL              IL V LLH   F +GY I+    F E   R IS+E GMQ
Sbjct: 323 KTQGAQL--------------ILPVALLHAAAFAIGYWISKF-SFGESTSRTISIECGMQ 382

Query: 343 NSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRNFE-PSDSQ 384
           +S+LG +LA  HF++ +VA+P+A+S V M + GS L  FWRN   P+D +
Sbjct: 383 SSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFWRNLPIPADDK 404

BLAST of Lsi05G007420 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 140.2 bits (352), Expect = 3.5e-33
Identity = 112/325 (34.46%), Postives = 167/325 (51.38%), Query Frame = 0

Query: 62  AGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQR 121
           + L P  +    + A   P +F+W        Y+ ALG +ML++G+ L + D F L  +R
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWV---SKDLYAPALGGIMLSIGIQLSVDD-FALAFKR 171

Query: 122 PL--------SYTIMPVVGALIGKFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDVP 181
           P+         Y + P++G L+    G+P +   G IL  C  G   SS  + +++ DV 
Sbjct: 172 PVPLSVGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVA 231

Query: 182 LSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACPE 241
           +SI++T  TT+ +VI TP L+  L+G+ +PVDA  +S S LQVV+VPI +G  L      
Sbjct: 232 MSILLTSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKP 291

Query: 242 LVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGELG 301
           +V  + P  P  A++ +SL                 + S LS N SQ      ILS E  
Sbjct: 292 VVTLLQPVMPFVAMVCTSL----------------CIGSPLSINRSQ------ILSAEGL 351

Query: 302 VVILAVFLLHLTGFFVGYIIAAIGGFRERER--RAISLEVGMQNSSLGVVLATAHFSSAM 361
            +I+ +   H   F +GY  + I G R+ E   R ISL  GMQ+S+L  +LA+    S+ 
Sbjct: 352 GLIVPIVTFHAVAFALGYWFSKIPGLRQEEEVSRTISLCTGMQSSTLAGLLASQFLGSSQ 409

Query: 362 VALPAAMSAVIMNMMGSSLGSFWRN 377
            A+PAA S V+M +MG  L SFW N
Sbjct: 412 -AVPAACSVVVMAIMGLCLASFWGN 409

BLAST of Lsi05G007420 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-28
Identity = 114/387 (29.46%), Postives = 182/387 (47.03%), Query Frame = 0

Query: 11  PLRLNPLLLPPSKPPGHLVIVRSLQRD--TEL---PSLPPPSQNQTNSLAKFVSTAAGLF 70
           PLR++P+    S+  G   + R    D  +E+   P   P    +  S+   +  A  + 
Sbjct: 46  PLRIDPI----SQVGGSRNLWRRYASDNFSEMGLDPGADPFKVIEKPSIVDRMKKANSIL 105

Query: 71  PLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTLQLKDLFNLFMQRPLS- 130
           P  +    I+A + P +F+WF  R    +  ALG +M A+G+    KD    F +RP + 
Sbjct: 106 PHVVLASTILALIYPPSFTWFTSR---YFVPALGFLMFAVGINSNEKDFLEAF-KRPKAI 165

Query: 131 -------YTIMPVVGALIG----KFLGLPPSLSVGLILLGCCPGGIASSVVTLIARGDV- 190
                  Y + PV+G + G        LP  +  G++L+ C  G   S+  T +    + 
Sbjct: 166 LLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQLSNYATFLTDPALA 225

Query: 191 PLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQVVVVPILVGSYLQKACP 250
           PLSI+MT  +T  AV++TP L+  L+G  +PVD   +  S LQVV+ PI  G  L K  P
Sbjct: 226 PLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSILQVVIAPIAAGLLLNKLFP 285

Query: 251 ELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSPNTSQLIGLKTILSGEL 310
           ++   I PF P+ +VL +   AC V                        + + +++S   
Sbjct: 286 KVSNAIRPFLPILSVLDT---ACCV-------------------GAPLALNINSVMSPFG 345

Query: 311 GVVILAVFLLHLTGFFVGYIIAAIGGFR-----ERERRAISLEVGMQNSSLGVVLATAHF 370
             ++L V + HL+ F  GY +     FR     +  +R +S E GMQ+S L + LAT  F
Sbjct: 346 ATILLLVTMFHLSAFLAGYFLTG-SVFRNAPDAKAMQRTLSYETGMQSSLLALALATKFF 401

Query: 371 SSAMVALPAAMSAVIMNMMGSSLGSFW 375
              +V +P A+S V+M++MG +L   W
Sbjct: 406 QDPLVGIPPAISTVVMSLMGFTLVLIW 401

BLAST of Lsi05G007420 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 116.3 bits (290), Expect = 5.5e-26
Identity = 103/344 (29.94%), Postives = 158/344 (45.93%), Query Frame = 0

Query: 50  QTNSLAKFVSTAAGLFPLYITGGGIVACVKPSTFSWFVQRGPGSYSLALGLVMLAMGLTL 109
           + +S+ + +  A    P  I    I+A V P +F+WF    P  +   LG +M A+G+  
Sbjct: 87  KVSSIMETLKQAYSFIPHGILLSTILALVYPPSFTWF---KPRYFVPGLGFMMFAVGINS 146

Query: 110 QLKDLFNLFMQRPLS--------YTIMPVVGALIG----KFLGLPPSLSVGLILLGCCPG 169
             +D     ++RP +        Y I P++G + G        LP S+  G++L+ C  G
Sbjct: 147 NERDFLEA-LKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSG 206

Query: 170 GIASSVVTLIARGDV-PLSIIMTVCTTLEAVILTPFLTKTLVGAFIPVDAAKLSLSTLQV 229
              S+  T +    +  LSI+MT  +T  AV++TP L+  L+G  +PVD   +  S LQV
Sbjct: 207 AQLSNYTTFLTDPSLAALSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFGMISSILQV 266

Query: 230 VVVPILVGSYLQKACPELVKRIIPFSPLFAVLTSSLLACSVFSENIIRLKSSLVSSTLSP 289
           V+ PI  G  L +  P L   I PF P   V+  S    +  + NI              
Sbjct: 267 VITPIAAGLLLNRLFPRLSNAIKPFLPALTVIDMSCCIGAPLALNI-------------- 326

Query: 290 NTSQLIGLKTILSGELGVVILAVFLLHLTGFFVGYIIAAIGGFRERE----RRAISLEVG 349
                    +ILS     ++  V   HL  F  GY        +  +    +R IS E G
Sbjct: 327 --------DSILSPFGATILFLVITFHLLAFVAGYFFTGFFFSKAPDVKALQRTISYETG 386

Query: 350 MQNSSLGVVLATAHFSSAMVALPAAMSAVIMNMMGSSLGSFWRN 377
           MQ+S L + LAT  F   +V +P A+S V+M++MG SL + W+N
Sbjct: 387 MQSSLLALALATKFFQDPLVGVPPAISTVVMSLMGVSLVTIWKN 404