Lsi05G007070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G007070
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	TIR domain-containing protein-like
Location	chr05: 9496434 .. 9499405 (-)
RNA-Seq Expression	Lsi05G007070
Synteny	Lsi05G007070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACCGTGTGGCGATCAGCGGTGTTGCGAACATTACGCCGAGGCAGTTTCTAGCTCACCTTGGGAAGAAGGAGATGAAGGGTATTGTGAAGCCTTGTGATGTGTTCATAAACCATAGAGGCGTCGACACGAAGAAGACGGTGGCAGCATTGCTGTACGACCGGCTGGTTCGGGTGAACCTGAGGCCATTTCTCGACTACAGGAATTTGAAGCCTGGAGAGAAGCTGTTCGATGAAATTCATGGTGCCATCAGACAGTGTAAAGTTGGTGTTGCTGTGTTTTCACCTCGTTATTGTGAGTCTTATTTTTGCCTTCATGAGTTGGCTATGATCATCGAGTCCAACAAGAAGGTATCTTACCTTTCTCTTCTATTATTATTATTATTATTATTATTATTATTATTATTATATTTTATTTTATTTTTTAGCATTGTTCGATAGAGTTTATCATTACCAATTCCATCTACCATAATTTTTTAGAAAAAAAATTATCAAAATTTAGGAGTATAAATTGATATTTGTACTAATTTAATATACATGTGATTTAGAAATATATCCCTATTTTGTTTATAGGTTTTTTAAATCAAACTTATAAAAGTGATTTGTTTTTAGGAAAAAGATCAATTTTGATAATCAAATGTACATATATTTTATAATTTATTGAATGGAAAAGAGAACTCTTAAATGATATATAAACATTTTAAAGTTTGGTTATGATATACATACATACATACATGGATTACTTTTCTTTCATGTATTTTGTTTTTTAAAAATGATTTTATGATCATTGTAGTTAAAGTTTAATTTTTTTTAGTAGAAAATAAAATTTTGAAAATTTAAATCTGGTTTGATAATCAGTTGATTTTTAAAAATTGTGTCAATTTTCTCACTAAGTACCCTTTGGATTTAGATTTTTCAAAAACAAAAAATCTAAATCCAAATGGTTATCAAATTAGGTGGTCTTCCTTTTATTTTAGTTTGAGAGGATATGCATTACTCCTTAAGCAAACCCTTATTTTCGAAACAATGTTTGTTTAGTTTATGAATTAGGGTTTTAGCTTTTGTTTATGATTGACTTTAGTTCTTTTTTTTTCTATCTTCAAAATTTAAATCTATAAAAATCGTTTGGATTTATATTGATTGAATACCTTTCAAAGCTTAGCTAACTTCAAAAAGAGGGACAAAAACAAAAAGGAATTCTGAAATTTCAATAGAACTTTTGAAAGTATATTAAAGTTACAAAGAAAAATGCTAGTAACTATAAACACCTGAAAAAAAAAAAAAACATGATCATCAATATGAGTCTATAGCTCAATCTACATAATTTCGATCGAGGTAAGATATTCAAATTTCTCATCCTCCATATTGTTAAACTAAATGTGCACTAAATCACATCATTGAATGCCAACATTAATCTTACACTAGTATTTGCCAATTTTACAAAGAGTGAAAATAACTATCCTGAAAATATGAAAAAAAATAATTTAATTTTTCATTCTTGAACAGGTCATTCCTATCTTTTGCGATATAAAAGCATCTGAGCTTCGCATAGAGGATAATCATGAATATGAGATTGATGAGCTGAGAAGGTTCAATTGGGCTCTTGAACGAGCTAAACGGTTTGATGGACTCAAATTTAATTCCTCAAAAGAGTAAGTAAGATGGAACAAAAATATATATATATATATATATATATATATGAATATAAAAGGGTTGTTTTCAAATATAGAAAAATGAGTCAAACTATTTACAAATATAAAACTTTTTTACAGTCTATCAGTGATAGAACCGCGATAGAATTCTATCGTTTTCTATCGCTCAAGCGATAGAATTTATTTGCTTGAGCGATAGAAAGCGATAGAATTCTATCGCAGTCTATCATTGATGGATTGTAAAAGTTTTCTATATTTATAAATAGTTTGATATTTTTTCTATTTATAGTAATTTCCTAATAGAAAATTTATTATATATATATATATATATATTTAAAAAGATGCATACGTAAATATTTTTAAATTAGGTTAAAATACTATCAGTGTTTTTTTTGCTATTTTCTATAAATAGTTTGACATTCTTTTATACGTGAAAATTTCTCAAAATTACTATTTGATTCAAATACTTTGAGTTCTATTTTATTTTCATTGATGTACTTTCAATTATTTTAATTTAGTTTAGTATTTTTAATAAATCTTAAAGTTACTCTATCTACTTCTAATAAATTTAGAAGTTAGTTTTTATCAAAATTGGATATACAATAATAACAATTTTTCATGCAATAAAAGACCCATGTAAATATATTTCTAAAGTTTATAAAGAAAATGTTGATGGAAATTAACTTCTTTTTTATTTTAAAAAATAACCATAGGAACTAATAATGTTAAAATTTATTTAGAGTATAACCTATATTAAAATGATCTTTTTTTTTTTATTAAGATGAGGTATGTGATTCCGAGTTTACTAGTAAATATGAGGTTTGTGATTCTAATTCCCTTACTCCTACTTGTTGTATTAAAAAAAATAGACCTTTTTTTTAATAAGATGAGAGTTTTTTTAAAAGGTTTATTTTTTTACAACGTCAACATATATTTTAAGCGGAGGTAGTGAACTGTAAAGGTCTTAACCCTAGAAGTAATTACTCAGCTGTGTTTTTCCCTTTTTCCTTTTTTCTTTTTTCTGAGAACAATTACTTCAGCTGTTTTGTGCAATGAAAGTTTGATCAAAAAAGGTGAATGCACAAAATGTAAAATTTTAAATTTAAATGATAGAATTGAAAAATAACACAAATTTTGTAAATAAAATAAAATAGTGCATCTTAATGAATCAAAGAATAAAGTAGGAATTATTGTTTCATAAATTTAGCAAAGTATTTTCCCCCCCTAATTGATTTATTTCATTATTTTCTTAGGACCTTATCAGAAGTTGTCACGAAGGCTTCAAATATCGTCATAAGGAGTTTGATTGAGATGGACTGA

mRNA sequence

ATGAACCGTGTGGCGATCAGCGGTGTTGCGAACATTACGCCGAGGCAGTTTCTAGCTCACCTTGGGAAGAAGGAGATGAAGGGTATTGTGAAGCCTTGTGATGTGTTCATAAACCATAGAGGCGTCGACACGAAGAAGACGGTGGCAGCATTGCTGTACGACCGGCTGGTTCGGGTGAACCTGAGGCCATTTCTCGACTACAGGAATTTGAAGCCTGGAGAGAAGCTGTTCGATGAAATTCATGGTGCCATCAGACAGTGTAAAGTTGGTGTTGCTGTGTTTTCACCTCGTTATTGTGAGTCTTATTTTTGCCTTCATGAGTTGGCTATGATCATCGAGTCCAACAAGAAGGTCATTCCTATCTTTTGCGATATAAAAGCATCTGAGCTTCGCATAGAGGATAATCATGAATATGAGATTGATGAGCTGAGAAGGACCTTATCAGAAGTTGTCACGAAGGCTTCAAATATCGTCATAAGGAGTTTGATTGAGATGGACTGA

Coding sequence (CDS)

ATGAACCGTGTGGCGATCAGCGGTGTTGCGAACATTACGCCGAGGCAGTTTCTAGCTCACCTTGGGAAGAAGGAGATGAAGGGTATTGTGAAGCCTTGTGATGTGTTCATAAACCATAGAGGCGTCGACACGAAGAAGACGGTGGCAGCATTGCTGTACGACCGGCTGGTTCGGGTGAACCTGAGGCCATTTCTCGACTACAGGAATTTGAAGCCTGGAGAGAAGCTGTTCGATGAAATTCATGGTGCCATCAGACAGTGTAAAGTTGGTGTTGCTGTGTTTTCACCTCGTTATTGTGAGTCTTATTTTTGCCTTCATGAGTTGGCTATGATCATCGAGTCCAACAAGAAGGTCATTCCTATCTTTTGCGATATAAAAGCATCTGAGCTTCGCATAGAGGATAATCATGAATATGAGATTGATGAGCTGAGAAGGACCTTATCAGAAGTTGTCACGAAGGCTTCAAATATCGTCATAAGGAGTTTGATTGAGATGGACTGA

Protein sequence

MNRVAISGVANITPRQFLAHLGKKEMKGIVKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDIKASELRIEDNHEYEIDELRRTLSEVVTKASNIVIRSLIEMD

Homology

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BLAST of Lsi05G007070 vs. ExPASy Swiss-Prot
Match: I1GTC2 (TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 5.8e-30
Identity = 64/116 (55.17%), Postives = 84/116 (72.41%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           +VFINHRGVDTK+TVA LLYDRL +  LR FLD  +++PG++L + I  AIR+C V VA+
Sbjct: 54  EVFINHRGVDTKRTVARLLYDRLAQAGLRGFLDNMSMRPGDRLEERIGSAIRECTVAVAI 113

Query: 94  FSPRYCESYFCLHELAMIIESNKKVIPIFCDIKASEL----RIEDNHEYEIDELRR 146
           FSP YC+S +CL ELAM++ES K +IPIF DIK S+L     + D+  Y   +L R
Sbjct: 114 FSPSYCDSEYCLRELAMLVESRKAIIPIFYDIKPSDLLLPQAVADSEVYLPRDLER 169

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BLAST of Lsi05G007070 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.0e-14
Identity = 48/140 (34.29%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVF++ RG DT+KT    L++ L+   +  F+D + LK G+ +  E+  AI + +  V V
Sbjct: 13  DVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVV 72

Query: 94  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDIKASELRIEDNHEYE---------- 153
           FS  Y  S +CL EL  I+E ++K    V+P+F D+  S +R + N EY           
Sbjct: 73  FSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVR-KQNGEYAVCFTKFEANL 132

Query: 154 IDELRRTL--SEVVTKASNI 158
           +D+  + L   E +TK +NI
Sbjct: 133 VDDRDKVLRWREALTKVANI 151

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BLAST of Lsi05G007070 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.7e-11
Identity = 38/102 (37.25%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVF++ RG DT+KT  + LY+ L    ++ F D + L+ G  +  E+  AI + +  + V
Sbjct: 13  DVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVV 72

Query: 94  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDIKASELR 132
           FS  Y  S +CL+EL  I+E   +    VIPIF D+  S +R
Sbjct: 73  FSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVR 114

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BLAST of Lsi05G007070 vs. ExPASy Swiss-Prot
Match: Q9SSN3 (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.8e-11
Identity = 36/102 (35.29%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVF++ RG DT+    + LY  LVR ++R F D + L+ G++   E+   I   +  V V
Sbjct: 10  DVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKELENGQRFSPELKSPIEVSRFAVVV 69

Query: 94  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDIKASELR 132
            S  Y  S +CL EL  I++  KK    V+PIF  ++ + +R
Sbjct: 70  VSENYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVR 111

[Close]

BLAST of Lsi05G007070 vs. ExPASy Swiss-Prot
Match: F4JNB7 (Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.6e-09
Identity = 33/102 (32.35%), Postives = 57/102 (55.88%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVF +  GVD +KT  + L + L   ++  F+D+  ++    +  E+  AIR+ ++ + +
Sbjct: 13  DVFPSFSGVDVRKTFLSHLIEALDGKSINTFIDH-GIERSRTIAPELISAIREARISIVI 72

Query: 94  FSPRYCESYFCLHELAMI----IESNKKVIPIFCDIKASELR 132
           FS  Y  S +CL+EL  I     +  + VIP+F D+  SE+R
Sbjct: 73  FSKNYASSTWCLNELVEIHKCFNDLGQMVIPVFYDVDPSEVR 113

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BLAST of Lsi05G007070 vs. ExPASy TrEMBL
Match: A0A5D3BWV6 (Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G001590 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 7.0e-71
Identity = 132/146 (90.41%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKG-IVKPCDVFINHRGVDTKKTVAALLYDRLVRV 60
           MNRVA+ GV NITPRQFLAH+GKKEMKG I+KPCDVFINHRGVDTKKTVAALLYDRLVRV
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGMILKPCDVFINHRGVDTKKTVAALLYDRLVRV 60

Query: 61  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
            LRPFLDY+NL+PGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI
Sbjct: 61  RLRPFLDYKNLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120

Query: 121 PIFCDIKASELRIEDNHEYEIDELRR 146
           PIFCDIKAS+LRIED+H YE DE+RR
Sbjct: 121 PIFCDIKASQLRIEDHHGYETDEVRR 146

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BLAST of Lsi05G007070 vs. ExPASy TrEMBL
Match: A0A5A7UXT6 (Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G005810 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.5e-70
Identity = 131/146 (89.73%), Postives = 139/146 (95.21%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKG-IVKPCDVFINHRGVDTKKTVAALLYDRLVRV 60
           MNRVA+ GV NITPRQFLAH+GKKEMKG I+KPCDVFINHRGVDTKKTVAALLYDRLVRV
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGMILKPCDVFINHRGVDTKKTVAALLYDRLVRV 60

Query: 61  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
            LRPFLDY+NL+PGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIES KKVI
Sbjct: 61  RLRPFLDYKNLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESKKKVI 120

Query: 121 PIFCDIKASELRIEDNHEYEIDELRR 146
           PIFCDIKAS+LRIED+H YE DE+RR
Sbjct: 121 PIFCDIKASQLRIEDHHGYETDEVRR 146

[Close]

BLAST of Lsi05G007070 vs. ExPASy TrEMBL
Match: A0A6J1J9V9 (TIR domain-containing protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483045 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 8.5e-69
Identity = 140/183 (76.50%), Postives = 150/183 (81.97%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKGIVKPCDVFINHRGVDTKKTVAALLYDRLVRVN 60
           MNR  +S VANITPRQFLAHLG   M  IVKPCDVFINHRGVDTKKTVAALLYDRLVR+N
Sbjct: 1   MNR--MSAVANITPRQFLAHLGNNHMP-IVKPCDVFINHRGVDTKKTVAALLYDRLVRMN 60

Query: 61  LRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIP 120
           LRPFLDY+NLKPGEKLFDEIHGAIR CKVGVAVFSPRYCESYFCLHELAMIIESNKK+IP
Sbjct: 61  LRPFLDYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIP 120

Query: 121 IFCDIKASELRIEDNHEYEIDELRR---------------------TLSEVVTKASNIVI 163
           IFCDIK SELRIED+++YEIDE+RR                     TL EVVTK SN+VI
Sbjct: 121 IFCDIKPSELRIEDSNDYEIDEVRRFNWALHEAKQFEGLKFNSFKETLPEVVTKISNVVI 180

[Close]

BLAST of Lsi05G007070 vs. ExPASy TrEMBL
Match: A0A6J1ERQ8 (TIR domain-containing protein-like OS=Cucurbita moschata OX=3662 GN=LOC111435930 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 8.5e-69
Identity = 138/178 (77.53%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 6   ISGVANITPRQFLAHLGKKEMKGIVKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFL 65
           +S VANITPRQFLAHLG   M  IVKPCDVFINHRGVDTKKTVAALLYDRLVR+NLRPFL
Sbjct: 1   MSAVANITPRQFLAHLGNNHMP-IVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLRPFL 60

Query: 66  DYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDI 125
           DY+NLKPGEKLFDEIHGAIR CKVGVAVFSPRYCESYFCLHELAMIIESNKK+IPIFCDI
Sbjct: 61  DYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPIFCDI 120

Query: 126 KASELRIEDNHEYEIDELRR---------------------TLSEVVTKASNIVIRSL 163
           K SELRIED+++YEIDELRR                     TL EVVTK SN+VIR+L
Sbjct: 121 KPSELRIEDDNDYEIDELRRFNWALQQAKQFECLKFNSFKETLPEVVTKISNVVIRNL 177

[Close]

BLAST of Lsi05G007070 vs. ExPASy TrEMBL
Match: A0A1S3BU97 (vesicle-associated protein 1-4 OS=Cucumis melo OX=3656 GN=LOC103493343 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.0e-66
Identity = 134/168 (79.76%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 21  LGKKEMKG-IVKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDE 80
           +GKKEMKG I+KPCDVFINHRGVDTKKTVAALLYDRLVRV LRPFLDY+NL+PGEKLFDE
Sbjct: 1   MGKKEMKGMILKPCDVFINHRGVDTKKTVAALLYDRLVRVRLRPFLDYKNLRPGEKLFDE 60

Query: 81  IHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDIKASELRIEDNHEYE 140
           IHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDIKAS+LRIED+H YE
Sbjct: 61  IHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPIFCDIKASQLRIEDHHGYE 120

Query: 141 IDELRR---------------------TLSEVVTKASNIVIRSLIEMD 167
            DE+RR                     TLSEVVTKASNI++RSLIEMD
Sbjct: 121 TDEVRRFNWALQQAKSFDGLKFDSSTETLSEVVTKASNIIMRSLIEMD 168

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BLAST of Lsi05G007070 vs. NCBI nr
Match: XP_011650563.1 (TIR domain-containing protein [Cucumis sativus] >KAE8650175.1 hypothetical protein Csa_011061 [Cucumis sativus])

HSP 1 Score: 300.4 bits (768), Expect = 9.3e-78
Identity = 150/188 (79.79%), Postives = 164/188 (87.23%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKGI-VKPCDVFINHRGVDTKKTVAALLYDRLVRV 60
           MNRVA+SGV NITPRQFLAHLGKKEMKG+ +KPCDVFINHRGVDTKKTVAALLYDRLVRV
Sbjct: 1   MNRVAVSGVGNITPRQFLAHLGKKEMKGMTLKPCDVFINHRGVDTKKTVAALLYDRLVRV 60

Query: 61  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
           NLRPFLDY+N+KPG+KLFDEIHGAIR+CKVGVAVFSPRYCESYFCLHELAM+ ESNKKVI
Sbjct: 61  NLRPFLDYKNMKPGDKLFDEIHGAIRKCKVGVAVFSPRYCESYFCLHELAMMFESNKKVI 120

Query: 121 PIFCDIKASELRIEDNHEYEIDELRR---------------------TLSEVVTKASNIV 167
           PIFCDIKAS+LRI+D+H+YEIDELRR                     TLSEVVTKASNI+
Sbjct: 121 PIFCDIKASQLRIDDHHDYEIDELRRFDWALQKARSFDGLKFDSSRETLSEVVTKASNII 180

[Close]

BLAST of Lsi05G007070 vs. NCBI nr
Match: XP_038904251.1 (TIR-only protein-like [Benincasa hispida])

HSP 1 Score: 280.4 bits (716), Expect = 1.0e-71
Identity = 135/146 (92.47%), Postives = 142/146 (97.26%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKG-IVKPCDVFINHRGVDTKKTVAALLYDRLVRV 60
           MNRV  +GVANITPRQFLAHLGKKEMK  IVKPCDVFINHRGVDTKKTVAALLYDRLVRV
Sbjct: 1   MNRVVGAGVANITPRQFLAHLGKKEMKSIIVKPCDVFINHRGVDTKKTVAALLYDRLVRV 60

Query: 61  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
           NLRPFLDY+NLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMI+ESNKKVI
Sbjct: 61  NLRPFLDYKNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIMESNKKVI 120

Query: 121 PIFCDIKASELRIEDNHEYEIDELRR 146
           PI+CDIKAS+LRIEDNH+YEIDEL+R
Sbjct: 121 PIYCDIKASQLRIEDNHDYEIDELKR 146

[Close]

BLAST of Lsi05G007070 vs. NCBI nr
Match: TYK03312.1 (vesicle-associated protein 1-4 [Cucumis melo var. makuwa])

HSP 1 Score: 276.6 bits (706), Expect = 1.4e-70
Identity = 132/146 (90.41%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKG-IVKPCDVFINHRGVDTKKTVAALLYDRLVRV 60
           MNRVA+ GV NITPRQFLAH+GKKEMKG I+KPCDVFINHRGVDTKKTVAALLYDRLVRV
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGMILKPCDVFINHRGVDTKKTVAALLYDRLVRV 60

Query: 61  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
            LRPFLDY+NL+PGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI
Sbjct: 61  RLRPFLDYKNLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120

Query: 121 PIFCDIKASELRIEDNHEYEIDELRR 146
           PIFCDIKAS+LRIED+H YE DE+RR
Sbjct: 121 PIFCDIKASQLRIEDHHGYETDEVRR 146

[Close]

BLAST of Lsi05G007070 vs. NCBI nr
Match: KAA0060682.1 (vesicle-associated protein 1-4 [Cucumis melo var. makuwa])

HSP 1 Score: 274.2 bits (700), Expect = 7.2e-70
Identity = 131/146 (89.73%), Postives = 139/146 (95.21%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKG-IVKPCDVFINHRGVDTKKTVAALLYDRLVRV 60
           MNRVA+ GV NITPRQFLAH+GKKEMKG I+KPCDVFINHRGVDTKKTVAALLYDRLVRV
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGMILKPCDVFINHRGVDTKKTVAALLYDRLVRV 60

Query: 61  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVI 120
            LRPFLDY+NL+PGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIES KKVI
Sbjct: 61  RLRPFLDYKNLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESKKKVI 120

Query: 121 PIFCDIKASELRIEDNHEYEIDELRR 146
           PIFCDIKAS+LRIED+H YE DE+RR
Sbjct: 121 PIFCDIKASQLRIEDHHGYETDEVRR 146

[Close]

BLAST of Lsi05G007070 vs. NCBI nr
Match: KAG6577380.1 (TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 270.8 bits (691), Expect = 7.9e-69
Identity = 141/183 (77.05%), Postives = 150/183 (81.97%), Query Frame = 0


Query: 1   MNRVAISGVANITPRQFLAHLGKKEMKGIVKPCDVFINHRGVDTKKTVAALLYDRLVRVN 60
           MNR  +S VANITPRQFLAHLG   M  IVKPCDVFINHRGVDTKKTVAALLYDRLVR+N
Sbjct: 2   MNR--MSAVANITPRQFLAHLGNNHMP-IVKPCDVFINHRGVDTKKTVAALLYDRLVRMN 61

Query: 61  LRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIP 120
           LRPFLDY+NLKPGEKLFDEIHGAIR CKVGVAVFSPRYCESYFCLHELAMIIESNKK+IP
Sbjct: 62  LRPFLDYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIP 121

Query: 121 IFCDIKASELRIEDNHEYEIDELRR---------------------TLSEVVTKASNIVI 163
           IFCDIK SELRIED+++YEIDELRR                     TL EVVTK SN+VI
Sbjct: 122 IFCDIKPSELRIEDSNDYEIDELRRFNWALQQAKQFECLKFNSFKETLPEVVTKISNVVI 181

[Close]

BLAST of Lsi05G007070 vs. TAIR 10
Match: AT1G52900.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 157.5 bits (397), Expect = 9.1e-39
Identity = 80/155 (51.61%), Postives = 101/155 (65.16%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVFINHRG DTK+ +A LLYD L   NLRPFLD +N+KPG+KLFD I+ AI   KV V V
Sbjct: 39  DVFINHRGSDTKRNIATLLYDNLKSRNLRPFLDSKNMKPGDKLFDHINNAILTSKVAVTV 98

Query: 94  FSPRYCESYFCLHELAMIIESNKKVIPIFCDIKASELRI--------------------- 153
           FSP YC+SYFCLHELA+I+ES K+VIPIFCDIK S+L +                     
Sbjct: 99  FSPNYCDSYFCLHELALIMESRKRVIPIFCDIKPSQLDVMIERVTCSDDEIQRFRWALQE 158

Query: 154 -EDNHEYEIDELRRTLSEVVTKASNIVIRSLIEMD 167
            +D      D  +  LSEVVT AS++++  L+E++
Sbjct: 159 AKDIVGLTFDSYKGNLSEVVTVASDVIVERLVELE 193

[Close]

BLAST of Lsi05G007070 vs. TAIR 10
Match: AT1G61105.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.2e-38
Identity = 76/156 (48.72%), Postives = 105/156 (67.31%), Query Frame = 0


Query: 32  PCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGV 91
           PCD+FINHRG+DTKKT++ LLYD   R  L  FLD ++LKPG++LF EI  AI+ C VG+
Sbjct: 28  PCDIFINHRGIDTKKTISGLLYDCFTRQRLTAFLDSKSLKPGDRLFVEIDMAIKACGVGI 87

Query: 92  AVFSPRYCESYFCLHELAMIIESNKKVIPIFCDIKASELRIEDNHEYEIDELRR------ 151
           AVFSPRYC+SYFCLHELA+++++ K+VIPIFCD+K SEL ++D+      E+RR      
Sbjct: 88  AVFSPRYCDSYFCLHELALLVKNKKRVIPIFCDVKPSELCVKDDKTRPAAEIRRFQLALE 147

Query: 152 ---------------TLSEVVTKASNIVIRSLIEMD 167
                            SE +  AS+ V ++L+E++
Sbjct: 148 EAKYTVGLTFDTSNGDWSEFLAMASDAVTKNLLEVE 183

[Close]

BLAST of Lsi05G007070 vs. TAIR 10
Match: AT1G17615.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 75.9 bits (185), Expect = 3.5e-14
Identity = 36/102 (35.29%), Postives = 60/102 (58.82%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVF++ RG DT++T  + LY  L+ + +R F D   L+ G ++  ++  AI   K+ V +
Sbjct: 16  DVFLSFRGKDTRRTFISFLYKELIEMGIRTFKDDVELQSGRRIASDLLAAIENSKIAVVI 75

Query: 94  FSPRYCESYFCLHELAMIIESNKK----VIPIFCDIKASELR 132
            S  Y  S +CL EL MI++  KK    V+PIF +++ + +R
Sbjct: 76  ISKNYSASPWCLQELVMIMDVEKKGSIIVMPIFYNVEPAHVR 117

[Close]

BLAST of Lsi05G007070 vs. TAIR 10
Match: AT1G72950.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 72.0 bits (175), Expect = 5.0e-13
Identity = 37/102 (36.27%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 34  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVGVAV 93
           DVF++ RG+DT+++  + LY  L+R N+R F D + LK G ++  E+  AI   +  V V
Sbjct: 12  DVFLSFRGLDTRRSFISFLYKELIRRNIRTFKDDKELKNGRRITPELIRAIEGSRFAVVV 71

Query: 94  FSPRYCESYFCLHELAMIIE----SNKKVIPIFCDIKASELR 132
            S  Y  S +CL EL  I++     + KV+PIF  +    +R
Sbjct: 72  VSVNYAASRWCLEELVKIMDFENMGSLKVMPIFYGVDPCHVR 113

[Close]

BLAST of Lsi05G007070 vs. TAIR 10
Match: AT4G09430.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 72.0 bits (175), Expect = 5.0e-13
Identity = 38/110 (34.55%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 30  VKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKV 89
           VK  DVF++ RG DT+  + + L+  LV V +R F D + L+ G+ + +++  AI+    
Sbjct: 12  VKEYDVFLSFRGADTRNNIVSYLHKALVDVGIRTFKDDKELEEGDIISEKLVNAIQTSWF 71

Query: 90  GVAVFSPRYCESYFCLHELAMIIESNKK----VIPIFCDIKASELRIEDN 136
            V V S +Y  S +CL EL  I+E + +    V+PIF  ++ S++R + N
Sbjct: 72  AVVVLSEKYVTSSWCLEELRHIMELSIQDDIIVVPIFYKVEPSDVRYQKN 121

The following BLAST results are available for this feature:

BLAST of Lsi05G007070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1GTC2	5.8e-30	55.17	TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=...	[more]
A0A290U7C4	2.0e-14	34.29	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	2.7e-11	37.25	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
Q9SSN3	7.8e-11	35.29	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR ...	[more]
F4JNB7	5.6e-09	32.35	Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=...	[more]

BLAST of Lsi05G007070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BWV6	7.0e-71	90.41	Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A5A7UXT6	3.5e-70	89.73	Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1J9V9	8.5e-69	76.50	TIR domain-containing protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483045 P...	[more]
A0A6J1ERQ8	8.5e-69	77.53	TIR domain-containing protein-like OS=Cucurbita moschata OX=3662 GN=LOC111435930...	[more]
A0A1S3BU97	1.0e-66	79.76	vesicle-associated protein 1-4 OS=Cucumis melo OX=3656 GN=LOC103493343 PE=4 SV=1	[more]

BLAST of Lsi05G007070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650563.1	9.3e-78	79.79	TIR domain-containing protein [Cucumis sativus] >KAE8650175.1 hypothetical prote...	[more]
XP_038904251.1	1.0e-71	92.47	TIR-only protein-like [Benincasa hispida]	[more]
TYK03312.1	1.4e-70	90.41	vesicle-associated protein 1-4 [Cucumis melo var. makuwa]	[more]
KAA0060682.1	7.2e-70	89.73	vesicle-associated protein 1-4 [Cucumis melo var. makuwa]	[more]
KAG6577380.1	7.9e-69	77.05	TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi05G007070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52900.1	9.1e-39	51.61	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G61105.1	1.2e-38	48.72	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G17615.1	3.5e-14	35.29	Disease resistance protein (TIR-NBS class)	[more]
AT1G72950.1	5.0e-13	36.27	Disease resistance protein (TIR-NBS class)	[more]
AT4G09430.1	5.0e-13	34.55	Disease resistance protein (TIR-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 32..157 e-value: 2.1E-13 score: 60.5
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 34..130 e-value: 2.3E-19 score: 69.8
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 31..158 score: 24.63632
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 22..151 e-value: 1.5E-30 score: 107.8
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 32..150
None	No IPR available	PANTHER	PTHR31008	COP1-INTERACTING PROTEIN-RELATED	coord: 28..145
None	No IPR available	PANTHER	PTHR31008:SF16	TOLL-INTERLEUKIN-RESISTANCE (TIR) DOMAIN FAMILY PROTEIN	coord: 28..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G007070.1	Lsi05G007070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity