Lsi05G006390 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G006390
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	gibberellin 2-beta-dioxygenase 8-like
Location	chr05: 7914941 .. 7915573 (+)
RNA-Seq Expression	Lsi05G006390
Synteny	Lsi05G006390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAATGGAGATTGAACCTCCATTAGAAGTAAGGTATTATGAGTTGATGAAAGAGGAAGTGGAGGGTAAAAATGGAAGATTAGTAGTTGAAAATAATGAATATAAGAGGGAAATAGTGGAAGAATGTGAAGAAGAAATAGAGTTACCAGTGATTGATTTAGGGGTTTTAAAGATGGGGAATTTAGAGAGAGAGAAATGCAAGAATGAGATAGTAGAAGCTGCAAGAAATTGGGGTTTTTTCCAAATCTTAAATCATGGAGTTCCTGAGAAAGTATTGAAGGCAATGATTTATGAGCAAAAGAAAGTGTTTAATGAACCTTTTGAAAAGAAAAGTTTGAGTAACTTTCTTAATTTGGCTGGAACTTACCGTTGGGGTAACCCTGTGGCTATTTCTCCAACTCAAATCTCTTGGTCTGAAGCTTTTCATATTGCTGTTTTGGAGGTTTCAACCTTGAACGATCATGTCAATCTCAGGTATATAACTCAACCTTATTTATTTATTTTTCTTTTTCACCTCAATTCTTTCTATTTTACCTTTCTACTTTGATACCCTCTTCCCTTCTTCTTCATCATCTTCAGTAATTTTCTTTTTCTTTTTCTTTTTTAATTATAAGAGCAATAGTGAAAGAGCTA

mRNA sequence

AGTAATGGAGATTGAACCTCCATTAGAAGTAAGGTATTATGAGTTGATGAAAGAGGAAGTGGAGGGTAAAAATGGAAGATTAGTAGTTGAAAATAATGAATATAAGAGGGAAATAGTGGAAGAATGTGAAGAAGAAATAGAGTTACCAGTGATTGATTTAGGGGTTTTAAAGATGGGGAATTTAGAGAGAGAGAAATGCAAGAATGAGATAGTAGAAGCTGCAAGAAATTGGGGTTTTTTCCAAATCTTAAATCATGGAGTTCCTGAGAAAGTATTGAAGGCAATGATTTATGAGCAAAAGAAAGTGTTTAATGAACCTTTTGAAAAGAAAAGTTTGAGTAACTTTCTTAATTTGGCTGGAACTTACCGTTGGGGTAACCCTGTGGCTATTTCTCCAACTCAAATCTCTTGGTCTGAAGCTTTTCATATTGCTGTTTTGGAGGTTTCAACCTTGAACGATCATGTCAATCTCAGAGCAATAGTGAAAGAGCTA

Coding sequence (CDS)

ATGGAGATTGAACCTCCATTAGAAGTAAGGTATTATGAGTTGATGAAAGAGGAAGTGGAGGGTAAAAATGGAAGATTAGTAGTTGAAAATAATGAATATAAGAGGGAAATAGTGGAAGAATGTGAAGAAGAAATAGAGTTACCAGTGATTGATTTAGGGGTTTTAAAGATGGGGAATTTAGAGAGAGAGAAATGCAAGAATGAGATAGTAGAAGCTGCAAGAAATTGGGGTTTTTTCCAAATCTTAAATCATGGAGTTCCTGAGAAAGTATTGAAGGCAATGATTTATGAGCAAAAGAAAGTGTTTAATGAACCTTTTGAAAAGAAAAGTTTGAGTAACTTTCTTAATTTGGCTGGAACTTACCGTTGGGGTAACCCTGTGGCTATTTCTCCAACTCAAATCTCTTGGTCTGAAGCTTTTCATATTGCTGTTTTGGAGGTTTCAACCTTGAACGATCATGTCAATCTCAGAGCAATAGTGAAAGAGCTA

Protein sequence

MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNLEREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGTYRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVKEL

Homology

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BLAST of Lsi05G006390 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 2.2e-26
Identity = 67/161 (41.61%), Postives = 95/161 (59.01%), Query Frame = 0


Query: 3   IEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMG-NLE 62
           ++PP    Y  L+  ++  K+  +      +    V    EE+ELPVID+  L  G   E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFS---VTAVVEEVELPVIDVSRLIDGAEEE 60

Query: 63  REKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGTY 122
           REKCK  I  A+R WGFFQ++NHG+   VL+ M  EQ +VF EPF+KKS S   + AG+Y
Sbjct: 61  REKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSY 120

Query: 123 RWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVKE 163
           RWG P A S  Q+SWSEAFH+ + ++S   D   L + +++
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEK 157

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BLAST of Lsi05G006390 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.9e-26
Identity = 61/120 (50.83%), Postives = 85/120 (70.83%), Query Frame = 0


Query: 45  IELPVIDLGVLKMG-NLEREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFN 104
           I+LPVIDL  L  G  ++R++C  ++V AA+ WGFFQI+NHG+P+ V + M+ E+KK+F+
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 105 EPFEKKSLSNFLNLA-GTYRWGNPVAISPTQISWSEAFHIAVLEVSTL-NDHVNLRAIVK 162
           +PF  K    F +L+  +YRWGNP A SP Q S SEAFHI + EVS + +D  NLR IV+
Sbjct: 98  QPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVE 157

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BLAST of Lsi05G006390 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.3e-21
Identity = 52/125 (41.60%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 44  EIELPVIDLGVLKMGNL-EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVF 103
           E +LP++DL  L  G   ER+ C   +  AA  WGFFQ+ NHGV  ++++ M  EQ ++F
Sbjct: 47  ERDLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLF 106

Query: 104 NEPFEKKSLSNFLNLAGTYRWGNPVAISPTQISWSEAFHIAVLEVS----TLNDHVNLRA 163
             PFE K  +  LN  G+YRWGNP A S   +SWSEAFH+ +  +S       D  +LR 
Sbjct: 107 RLPFETKEKAGLLN--GSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRG 166

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BLAST of Lsi05G006390 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.9e-18
Identity = 53/140 (37.86%), Postives = 80/140 (57.14%), Query Frame = 0


Query: 26  LVVENNEYKREIVEECEEEIELPVIDLGVLKMGNLEREK-CKNEIVEAARNWGFFQILNH 85
           L VE +    ++     +E ELPVIDL     G +E E+ C+ ++V AA  WGFFQ+ NH
Sbjct: 21  LFVEQHRLDMDMGAIDVDECELPVIDLA----GLMEAEQVCRADMVRAASEWGFFQVTNH 80

Query: 86  GVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLA-GTYRWGNPVAISPTQISWSEAFHIA 145
           GVP+ +L+ +   Q  VF  PF++K     L  +  +YRWG P A    Q+SWSEA+HI 
Sbjct: 81  GVPQALLRELHDAQVAVFRRPFQEKVTERLLGFSPESYRWGTPTAKCLEQLSWSEAYHIP 140

Query: 146 VLEVSTLNDHVNLRAIVKEL 164
            +     +  +  RA+++E+
Sbjct: 141 -MTTPRPSTSIRARAVIEEV 155

[Close]

BLAST of Lsi05G006390 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-09
Identity = 41/124 (33.06%), Postives = 64/124 (51.61%), Query Frame = 0


Query: 44  EIELPVIDLGVLKMGNLEREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFN 103
           +I +P+IDL  L  GN   E  K  I EA R WGFFQ++NHGV  +++ A     K  FN
Sbjct: 92  DINIPIIDLDSLFSGN---EDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFN 151

Query: 104 EPFEKKSLSNFLNLAGTYR-WGNPVAISPTQI-SWSEAF--HIAVLEVSTLNDHVNLRAI 163
            P E K +  + N   TY  +G+ + +    I  W++ +  H   L +   N   +L + 
Sbjct: 152 LPVEAKEV--YSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSN 210

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BLAST of Lsi05G006390 vs. ExPASy TrEMBL
Match: A0A6J1JAT6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483296 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.3e-65
Identity = 128/166 (77.11%), Postives = 145/166 (87.35%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           MEIEPPL+VRYY LMKEE E KNGR V E  E  RE+VEECEEE ELPVIDLG+LKMGN 
Sbjct: 3   MEIEPPLQVRYYALMKEESESKNGRWVGEEEE--REVVEECEEEEELPVIDLGLLKMGNS 62

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKCK+E+VEAARNWGFFQ+LNHGVPE+VLK M+YEQ+KVFN+PF KKSLSN LN+AGT
Sbjct: 63  EREKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGT 122

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLR----AIVKE 163
           YRWGNPVA+SP+QISWSEAFH+AVLEVS L +HV LR    A+VK+
Sbjct: 123 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLGEHVTLRPTMEAVVKK 166

[Close]

BLAST of Lsi05G006390 vs. ExPASy TrEMBL
Match: A0A6J1F0S8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438457 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.3e-64
Identity = 129/166 (77.71%), Postives = 143/166 (86.14%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           MEIEPPL VRYY LMKEE E KNGR V E  E  RE+VEECEEE ELPVIDLG+LKMGN 
Sbjct: 3   MEIEPPLHVRYYALMKEESESKNGRWVGEEEE--REVVEECEEEEELPVIDLGLLKMGNS 62

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKC +E+VEAARNWGFFQ+LNHGVPE+VLK M+YEQKKVFN+PF KKSLSN LNLAGT
Sbjct: 63  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 122

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLR----AIVKE 163
           YRWGNPVA+SP+QISWSEAFH+AVLEVS L +HV LR    A+VK+
Sbjct: 123 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLRPTMEAVVKK 166

[Close]

BLAST of Lsi05G006390 vs. ExPASy TrEMBL
Match: A0A1S3CJB6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103501620 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 4.0e-55
Identity = 122/169 (72.19%), Postives = 135/169 (79.88%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           MEIEPPLE RY EL KEE+   NGR ++E +E   E +EECEEEIELPVIDLG LK GNL
Sbjct: 1   MEIEPPLEERYNELKKEEL---NGRRIIEADE---EAIEECEEEIELPVIDLGQLKKGNL 60

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLS-NFLNLAG 120
           ERE+CK EIVEAA NWGFFQ++NHGV EKVL AMI EQKKVFN+PF  KSLS NFLNL  
Sbjct: 61  EREECKKEIVEAAMNWGFFQVINHGVAEKVLNAMINEQKKVFNQPFVNKSLSGNFLNLPS 120

Query: 121 T-YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLR----AIVKEL 164
           T YRWGNPVAIS +QISWSEAFHI VLEVSTLN H+ LR     +VK+L
Sbjct: 121 THYRWGNPVAISSSQISWSEAFHIPVLEVSTLNHHITLRTTMEGVVKKL 163

[Close]

BLAST of Lsi05G006390 vs. ExPASy TrEMBL
Match: A0A6J1JF15 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111484442 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.3e-55
Identity = 110/161 (68.32%), Postives = 133/161 (82.61%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           M+I+PPL+V Y  LMK+EVEGKNGR+  E        VEE  E  +LPVIDLG+LKMGNL
Sbjct: 1   MDIDPPLQVCYNALMKDEVEGKNGRVDDE--------VEETYE--DLPVIDLGLLKMGNL 60

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKCK EIVEAARNWGFFQ++NHGVPE+ LKA++YEQKKVF  PF +KSL NFLNL GT
Sbjct: 61  EREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAQKSLGNFLNLGGT 120

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVK 162
           YRWGNP+A+SP+Q+SWSEAFHI VL+VS+L +HV LR+ ++
Sbjct: 121 YRWGNPLALSPSQMSWSEAFHIGVLDVSSLEEHVTLRSTME 151

[Close]

BLAST of Lsi05G006390 vs. ExPASy TrEMBL
Match: A0A6J1FQ56 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447223 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.2e-54
Identity = 110/161 (68.32%), Postives = 132/161 (81.99%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           M+I+PPL+V Y  LMK+EVEGK+GR+  E        VEE  E  +LPVIDLG+LKMGNL
Sbjct: 15  MDIDPPLQVCYKALMKDEVEGKSGRVEDE--------VEETYE--DLPVIDLGLLKMGNL 74

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKCK EIVEAARNWGFFQ++NHGVPE+ LKA++YEQKKVF  PF  KSL NFLNL GT
Sbjct: 75  EREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGT 134

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVK 162
           YRWGNP+A+SP+QISWSEAFHIAVL+VS+L +H  LR+ ++
Sbjct: 135 YRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHATLRSTME 165

[Close]

BLAST of Lsi05G006390 vs. NCBI nr
Match: XP_038906005.1 (gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida])

HSP 1 Score: 280.4 bits (716), Expect = 9.8e-72
Identity = 135/161 (83.85%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           MEIEPPLEVRYY LMK+E+EGKNGRL  E  +   E+VEECE+EIELP+IDLG+LKMGNL
Sbjct: 3   MEIEPPLEVRYYALMKDEMEGKNGRLKAEEEDDHDEVVEECEDEIELPMIDLGLLKMGNL 62

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           E+EKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFN+PF  KSLSNFLNLAGT
Sbjct: 63  EKEKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNQPFANKSLSNFLNLAGT 122

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVK 162
           YRWGNPVAISPTQISWSEAFHIAVLEVS+LNDH+ LR+ ++
Sbjct: 123 YRWGNPVAISPTQISWSEAFHIAVLEVSSLNDHLTLRSTME 163

[Close]

BLAST of Lsi05G006390 vs. NCBI nr
Match: XP_022985245.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 257.3 bits (656), Expect = 8.9e-65
Identity = 128/166 (77.11%), Postives = 145/166 (87.35%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           MEIEPPL+VRYY LMKEE E KNGR V E  E  RE+VEECEEE ELPVIDLG+LKMGN 
Sbjct: 3   MEIEPPLQVRYYALMKEESESKNGRWVGEEEE--REVVEECEEEEELPVIDLGLLKMGNS 62

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKCK+E+VEAARNWGFFQ+LNHGVPE+VLK M+YEQ+KVFN+PF KKSLSN LN+AGT
Sbjct: 63  EREKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGT 122

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLR----AIVKE 163
           YRWGNPVA+SP+QISWSEAFH+AVLEVS L +HV LR    A+VK+
Sbjct: 123 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLGEHVTLRPTMEAVVKK 166

[Close]

BLAST of Lsi05G006390 vs. NCBI nr
Match: XP_022932133.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata] >KAG6577443.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015515.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 255.8 bits (652), Expect = 2.6e-64
Identity = 129/166 (77.71%), Postives = 143/166 (86.14%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           MEIEPPL VRYY LMKEE E KNGR V E  E  RE+VEECEEE ELPVIDLG+LKMGN 
Sbjct: 3   MEIEPPLHVRYYALMKEESESKNGRWVGEEEE--REVVEECEEEEELPVIDLGLLKMGNS 62

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKC +E+VEAARNWGFFQ+LNHGVPE+VLK M+YEQKKVFN+PF KKSLSN LNLAGT
Sbjct: 63  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 122

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLR----AIVKE 163
           YRWGNPVA+SP+QISWSEAFH+AVLEVS L +HV LR    A+VK+
Sbjct: 123 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEEHVTLRPTMEAVVKK 166

[Close]

BLAST of Lsi05G006390 vs. NCBI nr
Match: XP_023520421.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229.9 bits (585), Expect = 1.5e-56
Identity = 116/152 (76.32%), Postives = 130/152 (85.53%), Query Frame = 0


Query: 15  MKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNLEREKCKNEIVEAAR 74
           MK E E KNGR V E  E  RE+VEECEEE ELPVIDLG+LKMGN EREKC +E+VEAAR
Sbjct: 1   MKGESESKNGRWVGEEEE--REVVEECEEEEELPVIDLGLLKMGNSEREKCISEMVEAAR 60

Query: 75  NWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGTYRWGNPVAISPTQI 134
           NWGFFQ+LNHGVPE+VLK M+YEQKKVFN+PF KKSLSN LNLAGTYRWGNPVA+SP+QI
Sbjct: 61  NWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGTYRWGNPVAMSPSQI 120

Query: 135 SWSEAFHIAVLEVSTLNDHVNLR----AIVKE 163
           SWSEAFH+AVLEVS L +HV LR    A+VK+
Sbjct: 121 SWSEAFHMAVLEVSNLEEHVTLRPTMEAVVKK 150

[Close]

BLAST of Lsi05G006390 vs. NCBI nr
Match: XP_023547089.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.2 bits (570), Expect = 8.3e-55
Identity = 111/161 (68.94%), Postives = 133/161 (82.61%), Query Frame = 0


Query: 1   MEIEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMGNL 60
           M+I+PPL+V Y  LMK+EVEGK+GR+  E        VEE  E  +LPVIDLG+LKMGNL
Sbjct: 1   MDIDPPLQVCYNALMKDEVEGKSGRVEDE--------VEETYE--DLPVIDLGLLKMGNL 60

Query: 61  EREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGT 120
           EREKCK EIVEAARNWGFFQ++NHGVPE+ LKA++YEQKKVF  PF  KSL NFLNL GT
Sbjct: 61  EREKCKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGT 120

Query: 121 YRWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVK 162
           YRWGNP+A+SP+QISWSEAFHIAVL+VS+L +HV LR+ ++
Sbjct: 121 YRWGNPLALSPSQISWSEAFHIAVLDVSSLEEHVTLRSTME 151

[Close]

BLAST of Lsi05G006390 vs. TAIR 10
Match: AT4G21200.3 (gibberellin 2-oxidase 8 )

HSP 1 Score: 121.7 bits (304), Expect = 5.4e-28
Identity = 69/159 (43.40%), Postives = 93/159 (58.49%), Query Frame = 0


Query: 3   IEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMG-NLE 62
           ++PP    Y  L+  ++  K+  +      +    V    EE+ELPVID+  L  G   E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFS---VTAVVEEVELPVIDVSRLIDGAEEE 60

Query: 63  REKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGTY 122
           REKCK  I  A+R WGFFQ++NHG+   VL+ M  EQ +VF EPF+KKS S   + AG+Y
Sbjct: 61  REKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSY 120

Query: 123 RWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIV 161
           RWG P A S  Q+SWSEAFH+ + ++S   D   LR  V
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLRNFV 155

[Close]

BLAST of Lsi05G006390 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 120.2 bits (300), Expect = 1.6e-27
Identity = 67/161 (41.61%), Postives = 95/161 (59.01%), Query Frame = 0


Query: 3   IEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMG-NLE 62
           ++PP    Y  L+  ++  K+  +      +    V    EE+ELPVID+  L  G   E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFS---VTAVVEEVELPVIDVSRLIDGAEEE 60

Query: 63  REKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSNFLNLAGTY 122
           REKCK  I  A+R WGFFQ++NHG+   VL+ M  EQ +VF EPF+KKS S   + AG+Y
Sbjct: 61  REKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSY 120

Query: 123 RWGNPVAISPTQISWSEAFHIAVLEVSTLNDHVNLRAIVKE 163
           RWG P A S  Q+SWSEAFH+ + ++S   D   L + +++
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEK 157

[Close]

BLAST of Lsi05G006390 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 119.8 bits (299), Expect = 2.1e-27
Identity = 61/120 (50.83%), Postives = 85/120 (70.83%), Query Frame = 0


Query: 45  IELPVIDLGVLKMG-NLEREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFN 104
           I+LPVIDL  L  G  ++R++C  ++V AA+ WGFFQI+NHG+P+ V + M+ E+KK+F+
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 105 EPFEKKSLSNFLNLA-GTYRWGNPVAISPTQISWSEAFHIAVLEVSTL-NDHVNLRAIVK 162
           +PF  K    F +L+  +YRWGNP A SP Q S SEAFHI + EVS + +D  NLR IV+
Sbjct: 98  QPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVE 157

[Close]

BLAST of Lsi05G006390 vs. TAIR 10
Match: AT4G21200.2 (gibberellin 2-oxidase 8 )

HSP 1 Score: 81.6 bits (200), Expect = 6.2e-16
Identity = 46/112 (41.07%), Postives = 66/112 (58.93%), Query Frame = 0


Query: 3   IEPPLEVRYYELMKEEVEGKNGRLVVENNEYKREIVEECEEEIELPVIDLGVLKMG-NLE 62
           ++PP    Y  L+  ++  K+  +      +    V    EE+ELPVID+  L  G   E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFS---VTAVVEEVELPVIDVSRLIDGAEEE 60

Query: 63  REKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNEPFEKKSLSN 114
           REKCK  I  A+R WGFFQ++NHG+   VL+ M  EQ +VF EPF+KK++S+
Sbjct: 61  REKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKNISD 109

[Close]

BLAST of Lsi05G006390 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-10
Identity = 41/124 (33.06%), Postives = 64/124 (51.61%), Query Frame = 0


Query: 44  EIELPVIDLGVLKMGNLEREKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFN 103
           +I +P+IDL  L  GN   E  K  I EA R WGFFQ++NHGV  +++ A     K  FN
Sbjct: 92  DINIPIIDLDSLFSGN---EDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFN 151

Query: 104 EPFEKKSLSNFLNLAGTYR-WGNPVAISPTQI-SWSEAF--HIAVLEVSTLNDHVNLRAI 163
            P E K +  + N   TY  +G+ + +    I  W++ +  H   L +   N   +L + 
Sbjct: 152 LPVEAKEV--YSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSN 210

The following BLAST results are available for this feature:

BLAST of Lsi05G006390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	2.2e-26	41.61	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C6I4	2.9e-26	50.83	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q7XP65	6.3e-21	41.60	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	2.9e-18	37.86	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9SRM3	3.2e-09	33.06	Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1	[more]

BLAST of Lsi05G006390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JAT6	4.3e-65	77.11	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148329...	[more]
A0A6J1F0S8	1.3e-64	77.71	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438...	[more]
A0A1S3CJB6	4.0e-55	72.19	gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103501620 PE...	[more]
A0A6J1JF15	5.3e-55	68.32	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148444...	[more]
A0A6J1FQ56	1.2e-54	68.32	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]

BLAST of Lsi05G006390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906005.1	9.8e-72	83.85	gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida]	[more]
XP_022985245.1	8.9e-65	77.11	gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima]	[more]
XP_022932133.1	2.6e-64	77.71	gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata] >KAG6577443.1 Gibbere...	[more]
XP_023520421.1	1.5e-56	76.32	gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo]	[more]
XP_023547089.1	8.3e-55	68.94	gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi05G006390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.3	5.4e-28	43.40	gibberellin 2-oxidase 8	[more]
AT4G21200.1	1.6e-27	41.61	gibberellin 2-oxidase 8	[more]
AT1G50960.1	2.1e-27	50.83	gibberellin 2-oxidase 7	[more]
AT4G21200.2	6.2e-16	41.07	gibberellin 2-oxidase 8	[more]
AT3G11180.1	2.3e-10	33.06	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 16..36
None	No IPR available	PANTHER	PTHR47990:SF6	GIBBERELLIN 2-BETA-DIOXYGENASE	coord: 19..160
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 19..160
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 42..148
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..144 e-value: 5.8E-15 score: 56.1
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 8..159 e-value: 8.4E-18 score: 67.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G006390.1	Lsi05G006390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding