Lsi05G006380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G006380
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-binding EF-hand family protein
Location	chr05: 7873945 .. 7874313 (+)
RNA-Seq Expression	Lsi05G006380
Synteny	Lsi05G006380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCAAGAACCAAATCCCCCAATCGGAATCTGGATTCGAAGATTGGCTGCCGGCGATGGCCTACAACCTCGGTGGCGAGGGGCTGATCGGCGAACTCTGCAACGGATTCAATCTGCTGATGGACAGAGAGAAGGGAGTGATTAATTTCGAGAGCTTGAAGAGGAATGCGGCTGCGCTAGGGCTTGGGGATCTGACGGATGAGGAGCTTAGAGGAATGTTAAGGGAAGGAGATTTCGACGGCGATGGCGCTCTTAATCAGATGGAATTTTGTGTTCTTATGTTCAGATTAAGTCCTCAATTAATGGACGCTTCCCGCTACTACTTCCATGAATGTCTCCGCCGAGAATTTAGGGATTTTTCTTGA

mRNA sequence

ATGGCGTCCAAGAACCAAATCCCCCAATCGGAATCTGGATTCGAAGATTGGCTGCCGGCGATGGCCTACAACCTCGGTGGCGAGGGGCTGATCGGCGAACTCTGCAACGGATTCAATCTGCTGATGGACAGAGAGAAGGGAGTGATTAATTTCGAGAGCTTGAAGAGGAATGCGGCTGCGCTAGGGCTTGGGGATCTGACGGATGAGGAGCTTAGAGGAATGTTAAGGGAAGGAGATTTCGACGGCGATGGCGCTCTTAATCAGATGGAATTTTGTGTTCTTATGTTCAGATTAAGTCCTCAATTAATGGACGCTTCCCGCTACTACTTCCATGAATGTCTCCGCCGAGAATTTAGGGATTTTTCTTGA

Coding sequence (CDS)

ATGGCGTCCAAGAACCAAATCCCCCAATCGGAATCTGGATTCGAAGATTGGCTGCCGGCGATGGCCTACAACCTCGGTGGCGAGGGGCTGATCGGCGAACTCTGCAACGGATTCAATCTGCTGATGGACAGAGAGAAGGGAGTGATTAATTTCGAGAGCTTGAAGAGGAATGCGGCTGCGCTAGGGCTTGGGGATCTGACGGATGAGGAGCTTAGAGGAATGTTAAGGGAAGGAGATTTCGACGGCGATGGCGCTCTTAATCAGATGGAATTTTGTGTTCTTATGTTCAGATTAAGTCCTCAATTAATGGACGCTTCCCGCTACTACTTCCATGAATGTCTCCGCCGAGAATTTAGGGATTTTTCTTGA

Protein sequence

MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRDFS

Homology

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BLAST of Lsi05G006380 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.2e-39
Identity = 82/109 (75.23%), Postives = 91/109 (83.49%), Query Frame = 0


Query: 11  ESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA-LGLGDLTDE 70
           +  F D+LP MA NLGGEGLIGELCNGF LLMDREKGVI FESL+RNAAA LGLGDLTDE
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 71  ELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREF 119
           ++R M++EGDFD DGALNQMEFCVLMFRLSP LM+ASR    E +  EF
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Lsi05G006380 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-35
Identity = 76/116 (65.52%), Postives = 91/116 (78.45%), Query Frame = 0


Query: 4   KNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA-ALG 63
           +NQ PQ    F+D+ P MA  LGGEGLI E+C GF LLMD++KGVI FESL+RNA+  LG
Sbjct: 13  ENQEPQ----FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLG 72

Query: 64  LGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREF 119
           LGDLTD+++R M+ EGDFD DGALNQMEFCVLMFRLSP+LM+ASR    E +  EF
Sbjct: 73  LGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Lsi05G006380 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 1.6e-24
Identity = 53/107 (49.53%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 11  ESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEE 70
           E+ +ED LP MA  +  E  + ELC GF+LL D E+ +I  ESL+RN+  LG+  ++ E+
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 71  LRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRRE 118
            +GM+REGD DGDGALNQ EFCVLM RLSP++M+ +  +  + L +E
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Lsi05G006380 vs. ExPASy Swiss-Prot
Match: Q9R1K9 (Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-09
Identity = 36/86 (41.86%), Postives = 50/86 (58.14%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEELRG 73
           F D+L  M   +  +    E+   F L  D E G I+F++LKR A  LG  +LTDEEL+ 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQE 145

Query: 74  MLREGDFDGDGALNQMEFCVLMFRLS 100
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of Lsi05G006380 vs. ExPASy Swiss-Prot
Match: Q06827 (Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.4e-09
Identity = 36/86 (41.86%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEELRG 73
           FE++L  M   +G      E+   F L  D E G I+F++LKR A  LG  ++TDEEL+ 
Sbjct: 82  FEEFLQMMTAKMGERDSREEIMKAFRLFDDDETGKISFKNLKRVAKELG-ENMTDEELQE 141

Query: 74  MLREGDFDGDGALNQMEFCVLMFRLS 100
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 142 MIDEADRDGDGEVNEEEFFRIMKKTS 166

[Close]

BLAST of Lsi05G006380 vs. ExPASy TrEMBL
Match: A0A0A0L2H0 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081900 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 4.7e-56
Identity = 109/120 (90.83%), Postives = 114/120 (95.00%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           M  KNQIPQSESGF+DWLP MA NLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MTPKNQIPQSESGFQDWLPVMAGNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGDFDGDGALNQMEFCVLMFRLSP+LM+ASRY FHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Lsi05G006380 vs. ExPASy TrEMBL
Match: A0A6J1CSD5 (calcium-binding protein PBP1-like OS=Momordica charantia OX=3673 GN=LOC111013856 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.4e-54
Identity = 106/118 (89.83%), Postives = 111/118 (94.07%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           MA+KNQ PQS+SGFEDWLPAMA+ LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MAAKNQSPQSQSGFEDWLPAMAWKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREF 119
           LGLGDL+DEELRGML EGDFDGDGALNQMEFCVLMFRLSP LMDASRY+  E LRREF
Sbjct: 61  LGLGDLSDEELRGMLTEGDFDGDGALNQMEFCVLMFRLSPDLMDASRYWLQESLRREF 118

[Close]

BLAST of Lsi05G006380 vs. ExPASy TrEMBL
Match: A0A5A7SII1 (Calcium-binding protein PBP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00340 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.7e-53
Identity = 105/120 (87.50%), Postives = 111/120 (92.50%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           M S N IPQSESGF+DWLP MA  LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGD +GDGALNQMEFCVLMFRLSP+LM+ASRY FHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Lsi05G006380 vs. ExPASy TrEMBL
Match: A0A1S3CKV7 (calcium-binding protein PBP1-like OS=Cucumis melo OX=3656 GN=LOC103501619 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.7e-53
Identity = 105/120 (87.50%), Postives = 111/120 (92.50%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           M S N IPQSESGF+DWLP MA  LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGD +GDGALNQMEFCVLMFRLSP+LM+ASRY FHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Lsi05G006380 vs. ExPASy TrEMBL
Match: A0A6J1JCW0 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483323 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-52
Identity = 106/122 (86.89%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           MA  NQIPQSESGFEDWLP MA  LGGEGLIGELCNGFNLLMD EKGVIN ESLKRNAAA
Sbjct: 1   MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGD +DEELRGMLREGDFDGDGALNQMEFCVLMFRLSP+LMDASRY F + LRREF +
Sbjct: 61  LGLGDHSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRYCFQQSLRREFPN 120

Query: 121 FS 123
           FS
Sbjct: 121 FS 122

[Close]

BLAST of Lsi05G006380 vs. NCBI nr
Match: XP_038905287.1 (calcium-binding protein KRP1-like [Benincasa hispida])

HSP 1 Score: 242.3 bits (617), Expect = 2.2e-60
Identity = 117/122 (95.90%), Postives = 119/122 (97.54%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           MASKNQIPQSES FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGDLTDEELRGMLREGDFDGDGALN+MEFCVLMFRLSPQLM+ASRY FHECLRREFRD
Sbjct: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120

Query: 121 FS 123
           FS
Sbjct: 121 FS 122

[Close]

BLAST of Lsi05G006380 vs. NCBI nr
Match: XP_004152909.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN56165.1 hypothetical protein Csa_010073 [Cucumis sativus])

HSP 1 Score: 226.9 bits (577), Expect = 9.6e-56
Identity = 109/120 (90.83%), Postives = 114/120 (95.00%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           M  KNQIPQSESGF+DWLP MA NLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MTPKNQIPQSESGFQDWLPVMAGNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGDFDGDGALNQMEFCVLMFRLSP+LM+ASRY FHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Lsi05G006380 vs. NCBI nr
Match: XP_022144078.1 (calcium-binding protein PBP1-like [Momordica charantia])

HSP 1 Score: 219.5 bits (558), Expect = 1.5e-53
Identity = 106/118 (89.83%), Postives = 111/118 (94.07%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           MA+KNQ PQS+SGFEDWLPAMA+ LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MAAKNQSPQSQSGFEDWLPAMAWKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREF 119
           LGLGDL+DEELRGML EGDFDGDGALNQMEFCVLMFRLSP LMDASRY+  E LRREF
Sbjct: 61  LGLGDLSDEELRGMLTEGDFDGDGALNQMEFCVLMFRLSPDLMDASRYWLQESLRREF 118

[Close]

BLAST of Lsi05G006380 vs. NCBI nr
Match: XP_008463452.1 (PREDICTED: calcium-binding protein PBP1-like [Cucumis melo] >KAA0025444.1 calcium-binding protein PBP1-like [Cucumis melo var. makuwa] >TYK04738.1 calcium-binding protein PBP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 218.4 bits (555), Expect = 3.4e-53
Identity = 105/120 (87.50%), Postives = 111/120 (92.50%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           M S N IPQSESGF+DWLP MA  LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGD +GDGALNQMEFCVLMFRLSP+LM+ASRY FHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Lsi05G006380 vs. NCBI nr
Match: XP_022985284.1 (calcium-binding protein PBP1-like [Cucurbita maxima])

HSP 1 Score: 215.7 bits (548), Expect = 2.2e-52
Identity = 106/122 (86.89%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   MASKNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60
           MA  NQIPQSESGFEDWLP MA  LGGEGLIGELCNGFNLLMD EKGVIN ESLKRNAAA
Sbjct: 1   MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREFRD 120
           LGLGD +DEELRGMLREGDFDGDGALNQMEFCVLMFRLSP+LMDASRY F + LRREF +
Sbjct: 61  LGLGDHSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRYCFQQSLRREFPN 120

Query: 121 FS 123
           FS
Sbjct: 121 FS 122

[Close]

BLAST of Lsi05G006380 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 162.9 bits (411), Expect = 1.6e-40
Identity = 82/109 (75.23%), Postives = 91/109 (83.49%), Query Frame = 0


Query: 11  ESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA-LGLGDLTDE 70
           +  F D+LP MA NLGGEGLIGELCNGF LLMDREKGVI FESL+RNAAA LGLGDLTDE
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 71  ELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREF 119
           ++R M++EGDFD DGALNQMEFCVLMFRLSP LM+ASR    E +  EF
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Lsi05G006380 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 150.6 bits (379), Expect = 8.2e-37
Identity = 76/116 (65.52%), Postives = 91/116 (78.45%), Query Frame = 0


Query: 4   KNQIPQSESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA-ALG 63
           +NQ PQ    F+D+ P MA  LGGEGLI E+C GF LLMD++KGVI FESL+RNA+  LG
Sbjct: 13  ENQEPQ----FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLG 72

Query: 64  LGDLTDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRREF 119
           LGDLTD+++R M+ EGDFD DGALNQMEFCVLMFRLSP+LM+ASR    E +  EF
Sbjct: 73  LGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Lsi05G006380 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-25
Identity = 53/107 (49.53%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 11  ESGFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEE 70
           E+ +ED LP MA  +  E  + ELC GF+LL D E+ +I  ESL+RN+  LG+  ++ E+
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 71  LRGMLREGDFDGDGALNQMEFCVLMFRLSPQLMDASRYYFHECLRRE 118
            +GM+REGD DGDGALNQ EFCVLM RLSP++M+ +  +  + L +E
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Lsi05G006380 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 46.2 bits (108), Expect = 2.2e-05
Identity = 29/82 (35.37%), Postives = 40/82 (48.78%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEELRG 73
           F ++L  MA  +       EL   F +    + G I+   L+     LG   LTDEE+  
Sbjct: 66  FPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG-EKLTDEEVEE 125

Query: 74  MLREGDFDGDGALNQMEFCVLM 96
           M+RE D DGDG +N  EF  +M
Sbjct: 126 MIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of Lsi05G006380 vs. TAIR 10
Match: AT1G66410.2 (calmodulin 4 )

HSP 1 Score: 46.2 bits (108), Expect = 2.2e-05
Identity = 29/82 (35.37%), Postives = 40/82 (48.78%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAALGLGDLTDEELRG 73
           F ++L  MA  +       EL   F +    + G I+   L+     LG   LTDEE+  
Sbjct: 76  FPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG-EKLTDEEVEE 135

Query: 74  MLREGDFDGDGALNQMEFCVLM 96
           M+RE D DGDG +N  EF  +M
Sbjct: 136 MIREADVDGDGQINYEEFVKIM 156

The following BLAST results are available for this feature:

BLAST of Lsi05G006380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81831	2.2e-39	75.23	Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1	[more]
Q9LSQ6	1.2e-35	65.52	Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1	[more]
Q9ZPX9	1.6e-24	49.53	Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2	[more]
Q9R1K9	1.4e-09	41.86	Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1	[more]
Q06827	2.4e-09	41.86	Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1	[more]

BLAST of Lsi05G006380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2H0	4.7e-56	90.83	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081900 PE=...	[more]
A0A6J1CSD5	7.4e-54	89.83	calcium-binding protein PBP1-like OS=Momordica charantia OX=3673 GN=LOC111013856...	[more]
A0A5A7SII1	1.7e-53	87.50	Calcium-binding protein PBP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CKV7	1.7e-53	87.50	calcium-binding protein PBP1-like OS=Cucumis melo OX=3656 GN=LOC103501619 PE=4 S...	[more]
A0A6J1JCW0	1.1e-52	86.89	calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483323 PE...	[more]

BLAST of Lsi05G006380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905287.1	2.2e-60	95.90	calcium-binding protein KRP1-like [Benincasa hispida]	[more]
XP_004152909.1	9.6e-56	90.83	calcium-binding protein KRP1 [Cucumis sativus] >KGN56165.1 hypothetical protein ...	[more]
XP_022144078.1	1.5e-53	89.83	calcium-binding protein PBP1-like [Momordica charantia]	[more]
XP_008463452.1	3.4e-53	87.50	PREDICTED: calcium-binding protein PBP1-like [Cucumis melo] >KAA0025444.1 calciu...	[more]
XP_022985284.1	2.2e-52	86.89	calcium-binding protein PBP1-like [Cucurbita maxima]	[more]

BLAST of Lsi05G006380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27280.1	1.6e-40	75.23	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	8.2e-37	65.52	pinoid-binding protein 1	[more]
AT2G46600.1	1.1e-25	49.53	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	2.2e-05	35.37	calmodulin 4	[more]
AT1G66410.2	2.2e-05	35.37	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 45..97 e-value: 6.1E-18 score: 64.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 9.99435
None	No IPR available	GENE3D	1.10.238.10		coord: 11..121 e-value: 5.5E-19 score: 70.2
None	No IPR available	PANTHER	PTHR47319:SF6	CALCIUM-BINDING PROTEIN KRP1	coord: 9..121
IPR044205	Calcium-binding protein KIC/PBP1/KRP1	PANTHER	PTHR47319	CALCIUM-BINDING PROTEIN KIC	coord: 9..121
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 14..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G006380.1	Lsi05G006380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding