Lsi05G006150 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G006150 vs. ExPASy TrEMBL
Match: A0A6J1F0Y0 (uncharacterized protein LOC111438493 OS=Cucurbita moschata OX=3662 GN=LOC111438493 PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 7.2e-165
Identity = 326/416 (78.37%), Postives = 355/416 (85.34%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M S+ A+N QEKLASMLDQLYLE GIL KMIYKNKNQHRRSSYF+YLLQVRRDLRLLQAA
Sbjct: 1   MASSVAENRQEKLASMLDQLYLECGILHKMIYKNKNQHRRSSYFQYLLQVRRDLRLLQAA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KLEELV+SCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFME+LLGAARLLS+MVEPIFK
Sbjct: 61  KLEELVNSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMEQLLGAARLLSEMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLARTFFTGFCF+ILALLARIRVLVQQILLDVVS+FNMV+SISKKK VVTINQ
Sbjct: 121 AATEISILLARTFFTGFCFIILALLARIRVLVQQILLDVVSIFNMVASISKKKHVVTINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGIQVFREFYPTNDEFVLLECVW++DKFILQE KQEVAT NQEEHIGPNVS AAS V YQ
Sbjct: 181 EGIQVFREFYPTNDEFVLLECVWKDDKFILQENKQEVATNNQEEHIGPNVSSAASTVLYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDE--SELADANPSNEKGLEL 304
            L+SFL                                GD+E  ++ A+AN SN++ L+L
Sbjct: 241 SLESFL--------------------------------GDEEPATKQAEANQSNDEALDL 300

Query: 305 MKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGA 364
           MKMSKNDLLASPS R NDIS++D TETKD SISPA TSS+T +P+EGSSLVNSSPS+VGA
Sbjct: 301 MKMSKNDLLASPSKRVNDISVKDITETKDSSISPAATSSQTFVPREGSSLVNSSPSLVGA 360

Query: 365 KKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           KKL+SKRPAFVS+K P PIT+SAVGIQFNETK D+VEKEDPFF LLTGG  KSSLF
Sbjct: 361 KKLHSKRPAFVSIKPPSPITTSAVGIQFNETKADSVEKEDPFFALLTGGKRKSSLF 384

BLAST of Lsi05G006150 vs. ExPASy TrEMBL
Match: A0A6J1J4A8 (uncharacterized protein LOC111483286 OS=Cucurbita maxima OX=3661 GN=LOC111483286 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 7.4e-162
Identity = 324/416 (77.88%), Postives = 352/416 (84.62%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M S+EA+N QEKLASMLDQL LESGIL KMIYKNKNQHRRS YFRYLLQVRRDLRLLQAA
Sbjct: 1   MASSEAENRQEKLASMLDQLCLESGILHKMIYKNKNQHRRSFYFRYLLQVRRDLRLLQAA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KL+EL+SSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFME+LLGA+RLLS+MVEPIFK
Sbjct: 61  KLKELISSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMEQLLGASRLLSEMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLARTFFTGFCF+ILALLARI VLVQQILLDVVS+FNMV+SISKKK VVTINQ
Sbjct: 121 AATEISILLARTFFTGFCFIILALLARIWVLVQQILLDVVSIFNMVASISKKKHVVTINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGIQVFREFYPTNDEFVLLECVW+E KFILQE KQEVAT NQEEHIGPNVS AAS VRYQ
Sbjct: 181 EGIQVFREFYPTNDEFVLLECVWKEGKFILQENKQEVATNNQEEHIGPNVSSAASTVRYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDE--SELADANPSNEKGLEL 304
            L+SFL                                GDDE  ++ A+AN SNEKG++L
Sbjct: 241 SLESFL--------------------------------GDDEPATKQAEANQSNEKGVDL 300

Query: 305 MKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGA 364
           MKMSKNDLLASPS R N+IS++D TETKD SISPA TSS+T +P+ GSSLVNSSPS+VGA
Sbjct: 301 MKMSKNDLLASPSERVNEISVKDITETKDSSISPAATSSQTFMPRGGSSLVNSSPSLVGA 360

Query: 365 KKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           KKL+SKRPAFVS+K P PIT+SAVGIQFNETK D+VE EDPFF  LTGG  KSSLF
Sbjct: 361 KKLHSKRPAFVSIKPPNPITTSAVGIQFNETKADSVE-EDPFFAFLTGGKPKSSLF 383

BLAST of Lsi05G006150 vs. ExPASy TrEMBL
Match: A0A5A7T6Z8 (DUF4477 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002690 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 6.1e-156
Identity = 321/420 (76.43%), Postives = 346/420 (82.38%), Query Frame = 0

Query: 1   MGDSMGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRL 60
           MGDSM STEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRS YFRYLLQVRRDLRL
Sbjct: 1   MGDSMDSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSFYFRYLLQVRRDLRL 60

Query: 61  LQAAKLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVE 120
           L A KLEEL+SSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGA RLLSQ++ 
Sbjct: 61  LHAVKLEELLSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAVRLLSQVIF 120

Query: 121 PIFKAATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVV 180
            +  ++ EISILLARTFF GFCFVILALLARIRVLVQQILLDVV VFN VSSISKKKQV 
Sbjct: 121 LLSLSSFEISILLARTFFPGFCFVILALLARIRVLVQQILLDVVLVFNTVSSISKKKQVA 180

Query: 181 TINQEGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASA 240
            INQEGIQVFREFYPTNDEFVLLEC+WE DKFIL+EK QEVATKNQ EHIGP+VSLA SA
Sbjct: 181 AINQEGIQVFREFYPTNDEFVLLECIWEVDKFILKEKIQEVATKNQ-EHIGPDVSLATSA 240

Query: 241 VRYQKLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDESELADANPSNEKGL 300
           + YQKL+SFL                                GDDESE  +A+ SN+KGL
Sbjct: 241 IWYQKLQSFL--------------------------------GDDESEEGEAHQSNDKGL 300

Query: 301 ELMKMSKNDLLASPSGRENDISIED--NTETKDGSISPAETSSKT-LPQEGSSLVNSSPS 360
            LMK +KN LLASPSGR NDIS+ED  NTETKDG ISP +TSSKT LPQEGSSLVNSS  
Sbjct: 301 GLMKTTKNGLLASPSGRVNDISLEDNTNTETKDGLISPVKTSSKTFLPQEGSSLVNSSLP 360

Query: 361 IVGAKKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           +  AKK NSKR AFVSV+ PKPITS +VGIQFNE+KV++VEKE+PFF+LL GG AKSSLF
Sbjct: 361 MPVAKKSNSKRLAFVSVELPKPITSGSVGIQFNESKVNSVEKENPFFSLLNGGKAKSSLF 387

BLAST of Lsi05G006150 vs. ExPASy TrEMBL
Match: A0A6J1DA39 (uncharacterized protein LOC111019049 OS=Momordica charantia OX=3673 GN=LOC111019049 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 6.3e-153
Identity = 311/416 (74.76%), Postives = 345/416 (82.93%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M +++A+NL+EKLAS+L QL+LESGIL KMIYKNKNQHRRSSYFRYLLQVRRDLRLLQA 
Sbjct: 1   MAASDAENLEEKLASLLGQLHLESGILHKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAT 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KLEELVSSCFQVIDGKKPKQKIH LESLKRRKCEVGKYNFMERLLGAARLLS+MVEPIFK
Sbjct: 61  KLEELVSSCFQVIDGKKPKQKIHLLESLKRRKCEVGKYNFMERLLGAARLLSEMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLAR FFTGFCF+ILALLARIRVLVQQILL+VVSVFNMVSSIS+KK +V INQ
Sbjct: 121 AATEISILLARMFFTGFCFMILALLARIRVLVQQILLNVVSVFNMVSSISQKKHIVRINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGI+VFREF+PTNDEFVLLECVW+EDKF+LQE KQ++ T+N EEH GP+VS A SAVRYQ
Sbjct: 181 EGIEVFREFFPTNDEFVLLECVWKEDKFVLQETKQKIETRNWEEHPGPSVSSATSAVRYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDESEL--ADANPSNEKGLEL 304
            ++SFLE                                DDES++  ADAN S E G++L
Sbjct: 241 SIESFLE--------------------------------DDESDIKQADANQSIE-GVDL 300

Query: 305 MKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGA 364
           MKMSKNDLLAS       +S EDNT T+DGS+ PAETSSKT LPQEGS L+NSSPS VGA
Sbjct: 301 MKMSKNDLLAS-------LSKEDNTTTRDGSVCPAETSSKTLLPQEGSLLMNSSPSSVGA 360

Query: 365 KKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           KK +SKRPAFVSVK+PKPI SSAVGIQFNETKVD+  +ED FFTLLTGG AKSSLF
Sbjct: 361 KKSDSKRPAFVSVKNPKPIASSAVGIQFNETKVDSELEEDQFFTLLTGGGAKSSLF 376

BLAST of Lsi05G006150 vs. ExPASy TrEMBL
Match: A0A1S3CQT2 (uncharacterized protein LOC103503723 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503723 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 1.1e-144
Identity = 299/387 (77.26%), Postives = 319/387 (82.43%), Query Frame = 0

Query: 34  MIYKNKNQHRRSSYFRYLLQVRRDLRLLQAAKLEELVSSCFQVIDGKKPKQKIHFLESLK 93
           MIYKNKNQHRRS YFRYLLQVRRDLRLL A KLEEL+SSCFQVIDGKKPKQKIHFLESLK
Sbjct: 1   MIYKNKNQHRRSFYFRYLLQVRRDLRLLHAVKLEELLSSCFQVIDGKKPKQKIHFLESLK 60

Query: 94  RRKCEVGKYNFMERLLGAARLLSQMVEPIFKAATEISILLARTFFTGFCFVILALLARIR 153
           RRKCEVGKYNFMERLLGA RLLSQMVEPIFKAATEISILLARTFF GFCFVILALLARIR
Sbjct: 61  RRKCEVGKYNFMERLLGAVRLLSQMVEPIFKAATEISILLARTFFPGFCFVILALLARIR 120

Query: 154 VLVQQILLDVVSVFNMVSSISKKKQVVTINQEGIQVFREFYPTNDEFVLLECVWEEDKFI 213
           VLVQQILLDVV VFN VSSISKKKQV  INQEGIQVFREFYPTNDEFVLLEC+WE DKFI
Sbjct: 121 VLVQQILLDVVLVFNTVSSISKKKQVAAINQEGIQVFREFYPTNDEFVLLECIWEVDKFI 180

Query: 214 LQEKKQEVATKNQEEHIGPNVSLAASAVRYQKLKSFLEGKVFMVFILSAPFTHSPFFIDI 273
           L+EK QEVATKNQ EHIGP+VSLA SA+ YQKL+SFL                       
Sbjct: 181 LKEKIQEVATKNQ-EHIGPDVSLATSAIWYQKLQSFL----------------------- 240

Query: 274 WVCSFSCAVGDDESELADANPSNEKGLELMKMSKNDLLASPSGRENDISIED--NTETKD 333
                    GDDESE  +A+ SN+KGL LMK +KN LLASPSGR NDIS+ED  NTETKD
Sbjct: 241 ---------GDDESEEGEAHQSNDKGLGLMKTTKNGLLASPSGRVNDISLEDNTNTETKD 300

Query: 334 GSISPAETSSKT-LPQEGSSLVNSSPSIVGAKKLNSKRPAFVSVKHPKPITSSAVGIQFN 393
           G ISP +TSSKT LPQEGSSLVNSS  +  AKK NSKR AFVSV+ PKPITS +VGIQFN
Sbjct: 301 GLISPVKTSSKTFLPQEGSSLVNSSLPMPVAKKSNSKRLAFVSVELPKPITSGSVGIQFN 354

Query: 394 ETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           E+KV++VEKE+PFF+LL GG AKSSLF
Sbjct: 361 ESKVNSVEKENPFFSLLNGGKAKSSLF 354

BLAST of Lsi05G006150 vs. NCBI nr
Match: XP_038905505.1 (uncharacterized protein LOC120091509 [Benincasa hispida] >XP_038905506.1 uncharacterized protein LOC120091509 [Benincasa hispida] >XP_038905507.1 uncharacterized protein LOC120091509 [Benincasa hispida] >XP_038905508.1 uncharacterized protein LOC120091509 [Benincasa hispida] >XP_038905509.1 uncharacterized protein LOC120091509 [Benincasa hispida] >XP_038905511.1 uncharacterized protein LOC120091509 [Benincasa hispida])

HSP 1 Score: 615.9 bits (1587), Expect = 2.5e-172
Identity = 345/414 (83.33%), Postives = 354/414 (85.51%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M STEA N+QEKL SMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA
Sbjct: 1   MDSTEAANVQEKLVSMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KLEELVSSCFQVIDGKKPKQKIHFLESLKRRK EVGKYNFMERLLGAARLLSQMVEPIFK
Sbjct: 61  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKSEVGKYNFMERLLGAARLLSQMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFN VSSISKKKQVVTINQ
Sbjct: 121 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNTVSSISKKKQVVTINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGIQVFREFYPTNDEFVLLECVWE+DKFILQEK+QEVATKNQEEH+GPNVSLAASAVRYQ
Sbjct: 181 EGIQVFREFYPTNDEFVLLECVWEKDKFILQEKEQEVATKNQEEHVGPNVSLAASAVRYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDESELADANPSNEKGLELMK 304
           KLKSFLE                                DDESE ADA+ SNEKGL+LMK
Sbjct: 241 KLKSFLE--------------------------------DDESEQADADQSNEKGLDLMK 300

Query: 305 MSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGAKK 364
           MSK+DLL SP GR NDI +E      DGSI P ETSSKT LPQE SSLVNSSPS+VG KK
Sbjct: 301 MSKHDLLGSPRGRVNDIPVE------DGSIGPVETSSKTFLPQEDSSLVNSSPSMVGTKK 360

Query: 365 LNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           LN KRPAFVSVKHPKPI SSAVG QFNETK D VEKEDPFFTLLTGG AKSSLF
Sbjct: 361 LNWKRPAFVSVKHPKPIISSAVGFQFNETKADIVEKEDPFFTLLTGGKAKSSLF 376

BLAST of Lsi05G006150 vs. NCBI nr
Match: XP_004140752.1 (uncharacterized protein LOC101210672 [Cucumis sativus] >XP_011651188.1 uncharacterized protein LOC101210672 [Cucumis sativus] >KAE8650470.1 hypothetical protein Csa_010674 [Cucumis sativus])

HSP 1 Score: 601.7 bits (1550), Expect = 4.9e-168
Identity = 335/418 (80.14%), Postives = 354/418 (84.69%), Query Frame = 0

Query: 1   MGDSMGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRL 60
           MGDSMGSTEA  LQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRL
Sbjct: 1   MGDSMGSTEAGKLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRL 60

Query: 61  LQAAKLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVE 120
           LQA KLEEL+SSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGA RLLSQMVE
Sbjct: 61  LQAVKLEELLSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAVRLLSQMVE 120

Query: 121 PIFKAATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVV 180
           PIFKAATEISILLARTFF GFCFVILALLARIRVLVQQILLDVVSVFN VSSISKKKQVV
Sbjct: 121 PIFKAATEISILLARTFFPGFCFVILALLARIRVLVQQILLDVVSVFNTVSSISKKKQVV 180

Query: 181 TINQEGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASA 240
            INQE IQVFREFYPTNDE+VLLEC+WE DKFIL+EKK E+ATKNQEEHI P+ SLA SA
Sbjct: 181 AINQERIQVFREFYPTNDEYVLLECIWEVDKFILKEKKNEIATKNQEEHIAPDFSLATSA 240

Query: 241 VRYQKLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDESELADANPSNEKGL 300
           +RYQKL+SFL                            S    D ESE ADAN SNEKGL
Sbjct: 241 MRYQKLRSFLGD------------------------DESEEAEDHESEEADANESNEKGL 300

Query: 301 ELMKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIV 360
           +LMK + N LLASPSG  NDIS+EDNTETKDG ISP +T+SKT LPQEG+SLVNSSP + 
Sbjct: 301 DLMKTTPNGLLASPSGSVNDISMEDNTETKDGLISPVKTTSKTFLPQEGNSLVNSSPPMP 360

Query: 361 GAKKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
            AKK NSKRPAFVSV+ PKPITS  VGIQFNE+KVD+VEKE+PFFTLLTGG AKSSLF
Sbjct: 361 SAKKPNSKRPAFVSVELPKPITSGGVGIQFNESKVDSVEKENPFFTLLTGGKAKSSLF 394

BLAST of Lsi05G006150 vs. NCBI nr
Match: XP_023520444.1 (uncharacterized protein LOC111783829 [Cucurbita pepo subsp. pepo] >XP_023520445.1 uncharacterized protein LOC111783829 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 598.6 bits (1542), Expect = 4.2e-167
Identity = 331/416 (79.57%), Postives = 356/416 (85.58%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M S+ A+N QEKLASMLDQLYLE GIL KMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA
Sbjct: 1   MASSVAENRQEKLASMLDQLYLECGILHKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KLEELV+SCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFME+LLGAARLLS+MVEPIFK
Sbjct: 61  KLEELVNSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMEQLLGAARLLSEMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLARTFFTGFCF+ILALLARIRVLVQQILLDVVS+FNMV+SISKKK VVTINQ
Sbjct: 121 AATEISILLARTFFTGFCFIILALLARIRVLVQQILLDVVSIFNMVASISKKKHVVTINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGIQVFREFYPTNDEFVLLECVW+EDKFILQE KQEVAT NQEEHIGPNVS AAS VRYQ
Sbjct: 181 EGIQVFREFYPTNDEFVLLECVWKEDKFILQENKQEVATNNQEEHIGPNVSSAASTVRYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDE--SELADANPSNEKGLEL 304
            LKSFL                                GDDE  ++ A+AN SN++ L+L
Sbjct: 241 SLKSFL--------------------------------GDDEPATKQAEANQSNDEALDL 300

Query: 305 MKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGA 364
           MKMSKNDLLASPS R NDIS++D TETKD SISPA TSS+T +P+EGSSLVNSSPS+VGA
Sbjct: 301 MKMSKNDLLASPSKRVNDISVKDITETKDSSISPAATSSQTFVPREGSSLVNSSPSLVGA 360

Query: 365 KKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           KKL+SKRPAFVS+K P PIT+SAVGIQFNETK D+VEKEDPFF LLTGG  KSSLF
Sbjct: 361 KKLHSKRPAFVSIKPPNPITTSAVGIQFNETKADSVEKEDPFFALLTGGKRKSSLF 384

BLAST of Lsi05G006150 vs. NCBI nr
Match: KAG7015502.1 (hypothetical protein SDJN02_23138 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 595.5 bits (1534), Expect = 3.5e-166
Identity = 329/416 (79.09%), Postives = 356/416 (85.58%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M S+ A+N QEKLASMLDQLYLE GIL KMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA
Sbjct: 1   MASSVAENRQEKLASMLDQLYLECGILHKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KLEELV+SCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFME+LLGAARLLS+MVEPIFK
Sbjct: 61  KLEELVNSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMEQLLGAARLLSEMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLARTFFTGFCF+ILALLARIRVLVQQILLDVVS+FNMV+SISKKK VVTINQ
Sbjct: 121 AATEISILLARTFFTGFCFIILALLARIRVLVQQILLDVVSIFNMVASISKKKHVVTINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGIQVFREFYPTNDEFVLLECVW+EDKFILQE KQEVAT NQEEHIGPNVS  ASAVRYQ
Sbjct: 181 EGIQVFREFYPTNDEFVLLECVWKEDKFILQENKQEVATNNQEEHIGPNVSSTASAVRYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDE--SELADANPSNEKGLEL 304
            L+SFL                                GDDE  ++ A+AN SN++ L+L
Sbjct: 241 SLESFL--------------------------------GDDELATKQAEANQSNDEALDL 300

Query: 305 MKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGA 364
           MKM+KNDLLASPS R NDIS++D TETKD SISPA TSS+T +P+EGSSLVNSSPS+VGA
Sbjct: 301 MKMNKNDLLASPSKRVNDISVKDITETKDSSISPAATSSQTFVPREGSSLVNSSPSLVGA 360

Query: 365 KKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           KKL+SKRPAFVS+K P PIT+SAVGIQFNETK D+VEKEDPFF LLTGG  KSSLF
Sbjct: 361 KKLHSKRPAFVSIKLPNPITTSAVGIQFNETKADSVEKEDPFFALLTGGKRKSSLF 384

BLAST of Lsi05G006150 vs. NCBI nr
Match: XP_022932178.1 (uncharacterized protein LOC111438493 [Cucurbita moschata] >XP_022932179.1 uncharacterized protein LOC111438493 [Cucurbita moschata])

HSP 1 Score: 590.1 bits (1520), Expect = 1.5e-164
Identity = 326/416 (78.37%), Postives = 355/416 (85.34%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M S+ A+N QEKLASMLDQLYLE GIL KMIYKNKNQHRRSSYF+YLLQVRRDLRLLQAA
Sbjct: 1   MASSVAENRQEKLASMLDQLYLECGILHKMIYKNKNQHRRSSYFQYLLQVRRDLRLLQAA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
           KLEELV+SCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFME+LLGAARLLS+MVEPIFK
Sbjct: 61  KLEELVNSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMEQLLGAARLLSEMVEPIFK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AATEISILLARTFFTGFCF+ILALLARIRVLVQQILLDVVS+FNMV+SISKKK VVTINQ
Sbjct: 121 AATEISILLARTFFTGFCFIILALLARIRVLVQQILLDVVSIFNMVASISKKKHVVTINQ 180

Query: 185 EGIQVFREFYPTNDEFVLLECVWEEDKFILQEKKQEVATKNQEEHIGPNVSLAASAVRYQ 244
           EGIQVFREFYPTNDEFVLLECVW++DKFILQE KQEVAT NQEEHIGPNVS AAS V YQ
Sbjct: 181 EGIQVFREFYPTNDEFVLLECVWKDDKFILQENKQEVATNNQEEHIGPNVSSAASTVLYQ 240

Query: 245 KLKSFLEGKVFMVFILSAPFTHSPFFIDIWVCSFSCAVGDDE--SELADANPSNEKGLEL 304
            L+SFL                                GD+E  ++ A+AN SN++ L+L
Sbjct: 241 SLESFL--------------------------------GDEEPATKQAEANQSNDEALDL 300

Query: 305 MKMSKNDLLASPSGRENDISIEDNTETKDGSISPAETSSKT-LPQEGSSLVNSSPSIVGA 364
           MKMSKNDLLASPS R NDIS++D TETKD SISPA TSS+T +P+EGSSLVNSSPS+VGA
Sbjct: 301 MKMSKNDLLASPSKRVNDISVKDITETKDSSISPAATSSQTFVPREGSSLVNSSPSLVGA 360

Query: 365 KKLNSKRPAFVSVKHPKPITSSAVGIQFNETKVDTVEKEDPFFTLLTGGNAKSSLF 418
           KKL+SKRPAFVS+K P PIT+SAVGIQFNETK D+VEKEDPFF LLTGG  KSSLF
Sbjct: 361 KKLHSKRPAFVSIKPPSPITTSAVGIQFNETKADSVEKEDPFFALLTGGKRKSSLF 384

BLAST of Lsi05G006150 vs. TAIR 10
Match: AT1G50910.1 (unknown protein; Has 1105 Blast hits to 802 proteins in 217 species: Archae - 2; Bacteria - 177; Metazoa - 445; Fungi - 210; Plants - 58; Viruses - 6; Other Eukaryotes - 207 (source: NCBI BLink). )

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-66
Identity = 143/248 (57.66%), Postives = 179/248 (72.18%), Query Frame = 0

Query: 5   MGSTEADNLQEKLASMLDQLYLESGILQKMIYKNKNQHRRSSYFRYLLQVRRDLRLLQAA 64
           M  T+   L+EKL S L QL LE  + ++M+YKNKNQHRR SYF+YLL+VRR+LRLL+ A
Sbjct: 1   MDDTQVKALEEKLKSQLSQLELEQAVFERMVYKNKNQHRRCSYFQYLLKVRRELRLLRTA 60

Query: 65  KLEELVSSCFQVIDGKKPKQKIHFLESLKRRKCEVGKYNFMERLLGAARLLSQMVEPIFK 124
            +E ++  CF VI G+  KQKIH LESLK +K + GK N +ERLLGA RLLSQM EPI K
Sbjct: 61  NMEGMLRPCFHVISGRISKQKIHVLESLKLKKSDTGKPNILERLLGALRLLSQMTEPILK 120

Query: 125 AATEISILLARTFFTGFCFVILALLARIRVLVQQILLDVVSVFNMVSSISKKKQVVTINQ 184
           AA+ IS LLAR+FF GF    LALLAR+RVLVQQILLD VSVFN V+S S KKQ V I Q
Sbjct: 121 AASGISTLLARSFFIGFSVTFLALLARLRVLVQQILLDAVSVFNSVTSTSLKKQSVKIAQ 180

Query: 185 EGIQVFREFYPTNDEFV-LLECVWEEDKFILQEKKQEVA-TKNQEEHIGPNVSLAASAVR 244
           +G++VFREFYP  +E V LL+CVW+ DK++L E  Q    +K  E+++  +V+   S V+
Sbjct: 181 DGVEVFREFYPKEEECVTLLDCVWKTDKYVLLETLQNSENSKPMEKNVSEDVTTRDSLVQ 240

Query: 245 YQKLKSFL 251
           YQ   S L
Sbjct: 241 YQTSVSSL 248