Lsi05G006070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G006070
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	fatty acid desaturase 4, chloroplastic
Location	chr05: 7522817 .. 7523773 (+)
RNA-Seq Expression	Lsi05G006070
Synteny	Lsi05G006070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTATCTTAGCTCAAAACAAGTTCCTTCCGAGCTCCCGAACCCATGTTAGCCCATGCAGCTCTCCGATTCCGCGCAGAGTTGTCTATTGCATAGCCAACACCACGGTTAACGTTGTTGCTGCTGGTGCTACCACCACCAATCCGAAGCATGCCAATGGCCAACTAGTCATTGAACGTGAAGTTATATCTCCTACTCCACTTACATTGGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCGACATGGAGTCATCGTGCGTGGGTGGCCAGTGGGTGCACCACGGTGCTCGTCTCTTTGGCAAAATCAATAGTAGGTGCAGCCAGTTCACATATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGATCTTGGATCTGGAGTTTATCATTGGGGTATCGATAACTATGGTGATGCGTCAACTCCAATCTTCGGCACCCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCATGGACAATCACGAAGCGTCAGTTCGCCAACAATCTGTACGCCTTGGCACGAGCTGTGACCTTCACCGTACTTCCTATAAGCCTGGCATTTGATGATCCGGTTCTACAGGGTTTTGTTGGTGTGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCATGGTAAAAAGAGTGAACTACCGCCACTCGTGGTGGCGATGCAAGATGCTGGCCTGCTTGTGTCACGAATGCAGCATTCTGCACATCATCGTCCACCCTATAACAACAACTACTGCATAGTAAGTGGAATTTGGAATAAGCTTTTGGATGAAACAAAGGTTTTTGAGGCATTCGAGATGATCTTGTTCTTCAAACTTGGGTTGAGACCAAGATCCTGGACAGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTAGAACAAACTACAGCCCATTAA

mRNA sequence

ATGTCTATCTTAGCTCAAAACAAGTTCCTTCCGAGCTCCCGAACCCATGTTAGCCCATGCAGCTCTCCGATTCCGCGCAGAGTTGTCTATTGCATAGCCAACACCACGGTTAACGTTGTTGCTGCTGGTGCTACCACCACCAATCCGAAGCATGCCAATGGCCAACTAGTCATTGAACGTGAAGTTATATCTCCTACTCCACTTACATTGGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCGACATGGAGTCATCGTGCGTGGGTGGCCAGTGGGTGCACCACGGTGCTCGTCTCTTTGGCAAAATCAATAGTAGGTGCAGCCAGTTCACATATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGATCTTGGATCTGGAGTTTATCATTGGGGTATCGATAACTATGGTGATGCGTCAACTCCAATCTTCGGCACCCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCATGGACAATCACGAAGCGTCAGTTCGCCAACAATCTGTACGCCTTGGCACGAGCTGTGACCTTCACCGTACTTCCTATAAGCCTGGCATTTGATGATCCGGTTCTACAGGGTTTTGTTGGTGTGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCATGGTAAAAAGAGTGAACTACCGCCACTCGTGGTGGCGATGCAAGATGCTGGCCTGCTTGTGTCACGAATGCAGCATTCTGCACATCATCGTCCACCCTATAACAACAACTACTGCATAGTAAGTGGAATTTGGAATAAGCTTTTGGATGAAACAAAGGTTTTTGAGGCATTCGAGATGATCTTGTTCTTCAAACTTGGGTTGAGACCAAGATCCTGGACAGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTAGAACAAACTACAGCCCATTAA

Coding sequence (CDS)

ATGTCTATCTTAGCTCAAAACAAGTTCCTTCCGAGCTCCCGAACCCATGTTAGCCCATGCAGCTCTCCGATTCCGCGCAGAGTTGTCTATTGCATAGCCAACACCACGGTTAACGTTGTTGCTGCTGGTGCTACCACCACCAATCCGAAGCATGCCAATGGCCAACTAGTCATTGAACGTGAAGTTATATCTCCTACTCCACTTACATTGGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCGACATGGAGTCATCGTGCGTGGGTGGCCAGTGGGTGCACCACGGTGCTCGTCTCTTTGGCAAAATCAATAGTAGGTGCAGCCAGTTCACATATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGATCTTGGATCTGGAGTTTATCATTGGGGTATCGATAACTATGGTGATGCGTCAACTCCAATCTTCGGCACCCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCATGGACAATCACGAAGCGTCAGTTCGCCAACAATCTGTACGCCTTGGCACGAGCTGTGACCTTCACCGTACTTCCTATAAGCCTGGCATTTGATGATCCGGTTCTACAGGGTTTTGTTGGTGTGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCATGGTAAAAAGAGTGAACTACCGCCACTCGTGGTGGCGATGCAAGATGCTGGCCTGCTTGTGTCACGAATGCAGCATTCTGCACATCATCGTCCACCCTATAACAACAACTACTGCATAGTAAGTGGAATTTGGAATAAGCTTTTGGATGAAACAAAGGTTTTTGAGGCATTCGAGATGATCTTGTTCTTCAAACTTGGGTTGAGACCAAGATCCTGGACAGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTAGAACAAACTACAGCCCATTAA

Protein sequence

MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIEREVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTEPNSEWTEETEILEQTTAH

Homology

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BLAST of Lsi05G006070 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 8.3e-110
Identity = 188/294 (63.95%), Postives = 227/294 (77.21%), Query Frame = 0


Query: 21  SSPIPRRVVYCIANTTVNVVAA---GATTTNPKHANGQLVIEREVISPTPLTLVNSSTYY 80
           + P P R    +   TVN+  +      +T P+    +LV+E+ + SP PL+        
Sbjct: 37  TKPQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS-------- 96

Query: 81  SVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLEPALAALVGYILADLGS 140
              NDP+L+STW+HR WVA+GCTT+ VSLAKS++G   SH+ LEPALA   GYILADLGS
Sbjct: 97  ---NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGS 156

Query: 141 GVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFTVLPISL 200
           GVYHW IDNYGD STP+ GTQIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TFTVLP+ L
Sbjct: 157 GVYHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDL 216

Query: 201 AFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRP 260
           AF+DPV  GFV   + CI+FSQQFHAWAHG KS+LPPLVVA+QD GLLVSR QH+ HHR 
Sbjct: 217 AFNDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRA 276

Query: 261 PYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTEPNSEWTEETEI 312
           PYNNNYCIVSG WN +LDE+KVFEA EM+ +F+LG+RPRSW+EPNS+W EETEI
Sbjct: 277 PYNNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of Lsi05G006070 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.0e-100
Identity = 167/271 (61.62%), Postives = 207/271 (76.38%), Query Frame = 0


Query: 40  VAAGATTTNPKHANGQLVIEREVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGC 99
           V    TTTNP+  +  LV+E+ +++P PL         S  NDP+L+STW+HR WVA+G 
Sbjct: 28  VTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQSTWTHRLWVAAGS 87

Query: 100 TTVLVSLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQI 159
           TT+  S AKSI+G   SH+WL+PALA   GY+ ADLGSGVYHW IDNYG ASTPI G Q+
Sbjct: 88  TTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDNYGGASTPIVGAQL 147

Query: 160 EAFQGHHKWPWTITKRQFANNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQ 219
           EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+   FV   + CI+ SQ
Sbjct: 148 EASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHSFVSTFAFCILLSQ 207

Query: 220 QFHAWAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKV 279
           QFHAWAHG KS+LPPLV+A+QD GLLVSR  H  HH+ PYN+NYC+VSG WNK+LDE+ +
Sbjct: 208 QFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVVSGAWNKVLDESNL 267

Query: 280 FEAFEMILFFKLGLRPRSWTEPNSEWTEETE 311
           F+A EM LFF+ G+RP SW EPNS+WTEETE
Sbjct: 268 FKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

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BLAST of Lsi05G006070 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.2e-95
Identity = 161/261 (61.69%), Postives = 200/261 (76.63%), Query Frame = 0


Query: 44  ATTTNPKHANGQLVIEREVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVL 103
           +TT +  +   +LV+E+ +++P PL+           NDP+L+STW+HR WVA+GCTTV 
Sbjct: 31  STTNSQPNHEMKLVVEQRLVNP-PLS-----------NDPTLQSTWTHRLWVAAGCTTVF 90

Query: 104 VSLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQ 163
           VS +KSI+GA  SH+WLEP+LA   GYILADLGSGVYHW  DNYGD STP+ G  IE  Q
Sbjct: 91  VSFSKSIIGAFGSHLWLEPSLAGFAGYILADLGSGVYHWATDNYGDESTPLVGIHIEDSQ 150

Query: 164 GHHKWPWTITKRQFANNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHA 223
            HHK PWTITKRQFANNL+ +AR  T  VLP+ LAFDD V+ GFV + + C++F Q FHA
Sbjct: 151 DHHKCPWTITKRQFANNLHFMARGTTLIVLPLDLAFDDHVVHGFVSMFAFCVLFCQLFHA 210

Query: 224 WAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAF 283
           WAHG KS+LPPLVV +QD GLLVSR+ H  HHR PYNNNYC+VSG+WNK+LDE+ VF+A 
Sbjct: 211 WAHGTKSKLPPLVVGLQDIGLLVSRIHHMNHHRAPYNNNYCVVSGVWNKVLDESNVFKAM 270

Query: 284 EMILFFKLGLRPRSWTEPNSE 305
           EM+L+ +LG+RPRSWTEPN E
Sbjct: 271 EMVLYIQLGVRPRSWTEPNYE 279

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BLAST of Lsi05G006070 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.4e-18
Identity = 65/199 (32.66%), Postives = 88/199 (44.22%), Query Frame = 0


Query: 104 VSLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAF 163
           V+L   + G   +  WL  + A L+GY+ AD  SG  HW  D +G    P+ G   I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 164 QGHHKWPWTITKRQFA-----NNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMF 223
           + HH     IT+  F      N L +L  A+    LP+S          F+G     +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 224 SQQFHAWAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDET 283
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  NK L   
Sbjct: 162 TNQFHKWSH--MDSPPALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 284 KVFEAFEMILFFKLGLRPR 297
             F   E ++ +  GL PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

[Close]

BLAST of Lsi05G006070 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-11
Identity = 46/179 (25.70%), Postives = 78/179 (43.58%), Query Frame = 0


Query: 120 LEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAFQGHHKWPWTITKRQFA 179
           ++  +  ++GY+L+D   G+YHW  D Y    TPI G T I   + HH  P  + +    
Sbjct: 6   IDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFTDK 65

Query: 180 NNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVA 239
           + +   A+     + P+   F    L  F+      I  +   H + H    E P     
Sbjct: 66  DLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWATI 125

Query: 240 MQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRS 298
           +Q  G   S  +H  HH  P+  NYC V+   N  L++  ++   E  + +  G++PR+
Sbjct: 126 LQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPRA 181

[Close]

BLAST of Lsi05G006070 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.4e-168
Identity = 290/318 (91.19%), Postives = 302/318 (94.97%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+PIPRRVVYCIANTTVN VA     T+PKHA+GQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVA-----TSPKHADGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISPTPLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASSHIWL
Sbjct: 61  EVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLP+SLA DDP+L GFVG+CSGCIMFSQQFHAWAH KKSELPPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           +AGLLVSR QHS HHRPPYNNNYCIVSGIWNKLLDETK+FEA EMIL+FKLGLRPRSWTE
Sbjct: 241 NAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNSEWTEETEILEQTTAH
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Lsi05G006070 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.2e-165
Identity = 286/318 (89.94%), Postives = 298/318 (93.71%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+P PRRVVYCIANTTVN VA     T+ KH +GQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVA-----TSSKHGDGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISP+PLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWL
Sbjct: 61  EVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLP+SLAFDDP L GFVGVCSGCIMFSQQFHAWAHGKKSE+PPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSR QH+ HHRPPYNNNYCIVSGIWN+LLDETKVFEA EMIL+FKLGLRPRSWTE
Sbjct: 241 DAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNSEWTEETEILEQTTAH
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Lsi05G006070 vs. ExPASy TrEMBL
Match: A0A1S3CJN9 (fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.2e-164
Identity = 284/318 (89.31%), Postives = 297/318 (93.40%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+P PRRVVYCIANTTVN V      T+ KH +GQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVG-----TSSKHGDGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISP+PLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWL
Sbjct: 61  EVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLP+SLAFDDP L GFVGVCSGCIMFSQQFHAWAHGKKSE+PPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSR QH+ HHRPPYNNNYCIVSGIWN+LLDETKVFEA EMIL+FKLGLRPRSWTE
Sbjct: 241 DAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNSEWTEETEILEQTTAH
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Lsi05G006070 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.8e-160
Identity = 278/317 (87.70%), Postives = 297/317 (93.69%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSRT V+P S PIPR++VYC+AN TV+V AAGATTT PKHA+GQLVI+R
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVAN-TVDVAAAGATTTKPKHADGQLVIKR 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           E +SP PLTL  +STY+ VLNDPSLRSTW HRAWVASGCTTVL+SLAKSIVGAASSHIWL
Sbjct: 61  EFVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EP LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLPI+LAF DP+L GFV VCSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWN+LLDETKVFEAFEMILFFKLG+RPRSW+E
Sbjct: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSE 300

Query: 301 PNSEWTEETEILEQTTA 318
           PN+EWTEETE +EQTTA
Sbjct: 301 PNTEWTEETETVEQTTA 315

[Close]

BLAST of Lsi05G006070 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.9e-155
Identity = 270/318 (84.91%), Postives = 286/318 (89.94%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSIL QNKFL SS+THVS  S  +PR+ V CIANTTVNV AA      PKH +GQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           E +SPT   L NSSTYYSVLNDPSL+STWSHR WV SGCT VL+SLAKSI GAASSH WL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTFTVLPI+LA +DP+L GFV VCSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSR+QHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEA EMILFFKLG+RPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Lsi05G006070 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 296/318 (93.08%), Postives = 309/318 (97.17%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+PIPRRV YCIAN+TVN VAAGATTTNPKHA+GQL+IER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVN-VAAGATTTNPKHADGQLLIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL
Sbjct: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYG+ STPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           +YALARAVTF VLP+SLAFDDP+L GFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ
Sbjct: 181 MYALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSR QHSAHHRPPYNNNYCIVSGIWNK+LDETKVFEA EMILFFKLG+RPRSWTE
Sbjct: 241 DAGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNS WTEETEI+EQTTAH
Sbjct: 301 PNSAWTEETEIIEQTTAH 317

[Close]

BLAST of Lsi05G006070 vs. NCBI nr
Match: XP_004152938.1 (fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetical protein Csa_011564 [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 4.9e-168
Identity = 290/318 (91.19%), Postives = 302/318 (94.97%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+PIPRRVVYCIANTTVN VA     T+PKHA+GQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVA-----TSPKHADGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISPTPLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASSHIWL
Sbjct: 61  EVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLP+SLA DDP+L GFVG+CSGCIMFSQQFHAWAH KKSELPPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           +AGLLVSR QHS HHRPPYNNNYCIVSGIWNKLLDETK+FEA EMIL+FKLGLRPRSWTE
Sbjct: 241 NAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNSEWTEETEILEQTTAH
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Lsi05G006070 vs. NCBI nr
Match: KAA0033959.1 (fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desaturase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 590.9 bits (1522), Expect = 6.6e-165
Identity = 286/318 (89.94%), Postives = 298/318 (93.71%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+P PRRVVYCIANTTVN VA     T+ KH +GQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVA-----TSSKHGDGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISP+PLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWL
Sbjct: 61  EVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLP+SLAFDDP L GFVGVCSGCIMFSQQFHAWAHGKKSE+PPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSR QH+ HHRPPYNNNYCIVSGIWN+LLDETKVFEA EMIL+FKLGLRPRSWTE
Sbjct: 241 DAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNSEWTEETEILEQTTAH
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Lsi05G006070 vs. NCBI nr
Match: XP_008463431.1 (PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 589.0 bits (1517), Expect = 2.5e-164
Identity = 284/318 (89.31%), Postives = 297/318 (93.40%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSR+HV+PCS+P PRRVVYCIANTTVN V      T+ KH +GQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVG-----TSSKHGDGQLVIER 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           EVISP+PLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWL
Sbjct: 61  EVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLP+SLAFDDP L GFVGVCSGCIMFSQQFHAWAHGKKSE+PPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSR QH+ HHRPPYNNNYCIVSGIWN+LLDETKVFEA EMIL+FKLGLRPRSWTE
Sbjct: 241 DAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 319
           PNSEWTEETEILEQTTAH
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Lsi05G006070 vs. NCBI nr
Match: XP_022150986.1 (fatty acid desaturase 4, chloroplastic [Momordica charantia])

HSP 1 Score: 575.1 bits (1481), Expect = 3.8e-160
Identity = 278/317 (87.70%), Postives = 297/317 (93.69%), Query Frame = 0


Query: 1   MSILAQNKFLPSSRTHVSPCSSPIPRRVVYCIANTTVNVVAAGATTTNPKHANGQLVIER 60
           MSILAQNKFL SSRT V+P S PIPR++VYC+AN TV+V AAGATTT PKHA+GQLVI+R
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVAN-TVDVAAAGATTTKPKHADGQLVIKR 60

Query: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120
           E +SP PLTL  +STY+ VLNDPSLRSTW HRAWVASGCTTVL+SLAKSIVGAASSHIWL
Sbjct: 61  EFVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EP LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN
Sbjct: 121 EPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240
           LYALARAVTF VLPI+LAF DP+L GFV VCSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTE 300
           DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWN+LLDETKVFEAFEMILFFKLG+RPRSW+E
Sbjct: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSE 300

Query: 301 PNSEWTEETEILEQTTA 318
           PN+EWTEETE +EQTTA
Sbjct: 301 PNTEWTEETETVEQTTA 315

[Close]

BLAST of Lsi05G006070 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 398.3 bits (1022), Expect = 5.9e-111
Identity = 188/294 (63.95%), Postives = 227/294 (77.21%), Query Frame = 0


Query: 21  SSPIPRRVVYCIANTTVNVVAA---GATTTNPKHANGQLVIEREVISPTPLTLVNSSTYY 80
           + P P R    +   TVN+  +      +T P+    +LV+E+ + SP PL+        
Sbjct: 37  TKPQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS-------- 96

Query: 81  SVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLEPALAALVGYILADLGS 140
              NDP+L+STW+HR WVA+GCTT+ VSLAKS++G   SH+ LEPALA   GYILADLGS
Sbjct: 97  ---NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGS 156

Query: 141 GVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFTVLPISL 200
           GVYHW IDNYGD STP+ GTQIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TFTVLP+ L
Sbjct: 157 GVYHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDL 216

Query: 201 AFDDPVLQGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRP 260
           AF+DPV  GFV   + CI+FSQQFHAWAHG KS+LPPLVVA+QD GLLVSR QH+ HHR 
Sbjct: 217 AFNDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRA 276

Query: 261 PYNNNYCIVSGIWNKLLDETKVFEAFEMILFFKLGLRPRSWTEPNSEWTEETEI 312
           PYNNNYCIVSG WN +LDE+KVFEA EM+ +F+LG+RPRSW+EPNS+W EETEI
Sbjct: 277 PYNNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of Lsi05G006070 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 367.1 bits (941), Expect = 1.4e-101
Identity = 167/271 (61.62%), Postives = 207/271 (76.38%), Query Frame = 0


Query: 40  VAAGATTTNPKHANGQLVIEREVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGC 99
           V    TTTNP+  +  LV+E+ +++P PL         S  NDP+L+STW+HR WVA+G 
Sbjct: 28  VTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQSTWTHRLWVAAGS 87

Query: 100 TTVLVSLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQI 159
           TT+  S AKSI+G   SH+WL+PALA   GY+ ADLGSGVYHW IDNYG ASTPI G Q+
Sbjct: 88  TTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDNYGGASTPIVGAQL 147

Query: 160 EAFQGHHKWPWTITKRQFANNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQ 219
           EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+   FV   + CI+ SQ
Sbjct: 148 EASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHSFVSTFAFCILLSQ 207

Query: 220 QFHAWAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKV 279
           QFHAWAHG KS+LPPLV+A+QD GLLVSR  H  HH+ PYN+NYC+VSG WNK+LDE+ +
Sbjct: 208 QFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVVSGAWNKVLDESNL 267

Query: 280 FEAFEMILFFKLGLRPRSWTEPNSEWTEETE 311
           F+A EM LFF+ G+RP SW EPNS+WTEETE
Sbjct: 268 FKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

[Close]

BLAST of Lsi05G006070 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 351.3 bits (900), Expect = 8.2e-97
Identity = 161/261 (61.69%), Postives = 200/261 (76.63%), Query Frame = 0


Query: 44  ATTTNPKHANGQLVIEREVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVL 103
           +TT +  +   +LV+E+ +++P PL+           NDP+L+STW+HR WVA+GCTTV 
Sbjct: 31  STTNSQPNHEMKLVVEQRLVNP-PLS-----------NDPTLQSTWTHRLWVAAGCTTVF 90

Query: 104 VSLAKSIVGAASSHIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQ 163
           VS +KSI+GA  SH+WLEP+LA   GYILADLGSGVYHW  DNYGD STP+ G  IE  Q
Sbjct: 91  VSFSKSIIGAFGSHLWLEPSLAGFAGYILADLGSGVYHWATDNYGDESTPLVGIHIEDSQ 150

Query: 164 GHHKWPWTITKRQFANNLYALARAVTFTVLPISLAFDDPVLQGFVGVCSGCIMFSQQFHA 223
            HHK PWTITKRQFANNL+ +AR  T  VLP+ LAFDD V+ GFV + + C++F Q FHA
Sbjct: 151 DHHKCPWTITKRQFANNLHFMARGTTLIVLPLDLAFDDHVVHGFVSMFAFCVLFCQLFHA 210

Query: 224 WAHGKKSELPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEAF 283
           WAHG KS+LPPLVV +QD GLLVSR+ H  HHR PYNNNYC+VSG+WNK+LDE+ VF+A 
Sbjct: 211 WAHGTKSKLPPLVVGLQDIGLLVSRIHHMNHHRAPYNNNYCVVSGVWNKVLDESNVFKAM 270

Query: 284 EMILFFKLGLRPRSWTEPNSE 305
           EM+L+ +LG+RPRSWTEPN E
Sbjct: 271 EMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:

BLAST of Lsi05G006070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ42	8.3e-110	63.95	Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P...	[more]
O04584	2.0e-100	61.62	Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O81006	1.2e-95	61.69	Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9AE87	4.4e-18	32.66	Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1	[more]
Q5UR78	3.9e-11	25.70	Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=...	[more]

BLAST of Lsi05G006070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6B9	2.4e-168	91.19	TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07...	[more]
A0A5A7SXQ7	3.2e-165	89.94	Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CJN9	1.2e-164	89.31	fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 P...	[more]
A0A6J1DD70	1.8e-160	87.70	fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IGF1	3.9e-155	84.91	fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767...	[more]

BLAST of Lsi05G006070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905900.1	1.5e-172	93.08	fatty acid desaturase 4, chloroplastic-like [Benincasa hispida]	[more]
XP_004152938.1	4.9e-168	91.19	fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetica...	[more]
KAA0033959.1	6.6e-165	89.94	fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desatu...	[more]
XP_008463431.1	2.5e-164	89.31	PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo]	[more]
XP_022150986.1	3.8e-160	87.70	fatty acid desaturase 4, chloroplastic [Momordica charantia]	[more]

BLAST of Lsi05G006070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27030.1	5.9e-111	63.95	fatty acid desaturase A	[more]
AT1G62190.1	1.4e-101	61.62	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]
AT2G22890.1	8.2e-97	61.69	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019547	B domain of TMEM189, localisation domain	PFAM	PF10520	TMEM189_B_dmain	coord: 130..298 e-value: 1.6E-63 score: 213.9
None	No IPR available	PANTHER	PTHR48140	FAMILY NOT NAMED	coord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G006070.1	Lsi05G006070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006631	fatty acid metabolic process
cellular_component	GO:0016021	integral component of membrane