Lsi05G005740 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G005740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionEndoglucanase
Locationchr05: 7137228 .. 7140909 (+)
RNA-Seq ExpressionLsi05G005740
SyntenyLsi05G005740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGCATCAAAAGCAATTGCCTTTCTCATCTTCTTTCTTCTCATCCTTCCTTTAACCCATTCCTTGTTCGACTATCACCAAGCTCTCTCCATTAGTCTCCTCTACTTTGAGTCTCAACGCTCCGGTCACCTCCCCTACAACCAACGACTCACTTGGCGCCACCATTCCGGCCTCTCTGATGGCCTCCGTCAAGGAGTACACTTCCTTCACTCATTTTTAAATATTTACTATATGCGCATTTACTTCTTGTACTTTTAATTATGATAAATATTTAAACATCATCTTGAACTGTATTTATCTTTGTACAATATAATATATCTTATAATAAATACTTTTGCTGTGTGATTGGAGTGGGTTGCATGAGATGGGTGAGATTAAGAGAATTATTTCAAACTAAGTTGATTTTTTCAGGTGGACTTAGTGGGAGGGTACTATGATGCCGGCGACAATGTGAAGTTCGGTTTACCAATGGCATTCACCATCACAATGTTGTCGTGGGGTGTCATTGAATATGGAGAACAAATTGCAGCCGCCGAAGAGTACACTCATGCGCTAGAGGCTATCAAGTGGGGTACTGATTACTTCATCAAAGCTCACCCGCACCCAAACGTCTTCTGGGCACAGGTAAGTAGTTGCATTATGAGCAATATTGGTGTAGCTCAGGCTACACCATCTCAATGCATCTCTTATCACAAATAAAAAATTAGAACTTGTAATTTAACATTTGAGTAGACTGCACAAAGATAAATACAACTCGGATGCACCCAAATTTTTTTCTTATAGTGTACAAATCTTAGGCAAAAGTGTGACTTCCACGCACTCCACATTGGTCATTATAACGATCAAGGTTTCAACTTTTGCAGGTGGGTGACGGGGCAACGGATCACTACTGCTGGCAGCGGCCGGAGGACATGACAACGTCTCGACGAGCCTACAAGATCGACGAAAACAACCCGGGTTCCGATGTTGCCGGAGAGACGGCGGCGGCAATGGCAGCTGCAGCAATTGTTTTCAGTAGAACAAACCCACATTACTCGCATCTACTACTCCACCATGCACAAGAGGCAAGTTGAACAACTAATTAATAAGTAAAATAAAATGGAAGTGTTAATTAAATGAAGGAAATTAGAGGGAGCTGTTTGAAAAGTTAGTTGAGGGAGTTAATAAAAAGCATAAAATAAGGTAGTTAATTAATTTATATGTGGTTTAATAAAATAATTTTTGTAATGCAGTTGTTTGAATTTGGTGATAAGTACAGAGGGAAATATGATGAGAGCATTGAAGTGGTAAAAGGGTATTATTCGTCTGTGAGTGGGTACATGGATGAGCTACTGTGGGGAGCAATGTGGCTATACAAAGCAAGTGGAAGGGAAGAATATTTAAAATATGCGTTGGAAAATGGTTTGAATTTTGGTGGGATTAGTTGGGCCATCAATGAGTTCAGTTGGGATGTTAAGTATGCTGGTCTCCAAATCCTTGCATCCATGGTATCTTCCTATCTATTTTAAATATAGCAATTAGATTTAAAATATTAGTATATATAACAATATTTTTAAAAAAATTATAAATATAGCAAAATCTATCAATGATAGACTTTTATAGTCTAAGTGATCTATCGCTTATAGACCATTAGAGTCAGCGATAGAAATCTATCACAGATAGACTTTGCTATATTTGCACTTTTTTAAAAATGTTGTTATGCATTTGGTTATTATCCCTAAAATTGCTACTCATTATAATTACCAATATTTATATAACATTAAGTAATTAATGTCTTTGGGCCCCTGATTTTCAACTTGTAATTGTTCTTTTTAAGTAGGTTTAAATACTGCTGTAGTTTCTATACTTTTGATTTTTGTTCATTTTGGTTCTTGTAATTTCAAAAGGTTTATTTTGGTCTTATACTTTGAATTTTGGTTCATTTTGGTTTCTATACTCTTAAAACATTCATTTTGATCCTTGGGCTTTCAACTTTGGTTCATTTGGTCTTTGAACTTTTAAAAAGTTACCATTTTGGTACCTTAAAAATGATATAAAATGAAAATGAAGAGACCAAAATGGTTATTTTTTAAAAGGTAGAAAGACCAAAATGAACCAAAGTTAAAAGTATAGAGACCAAACTGAACATATTGAAAGTATATAGACAAAAATGAACTAAAATCAAAAGTATCGGAACTAAAATAGTATTTAAACCTTTTAATTACTAAAATACAAACTAAGTTGTCAACCAATAATTAGTCTTTAACTACAATTAGTTTTATGATTTATAAATATAGTATCAAACGCATTTACGACAAGTGTTATGATTTTTGGATCTACTGCTAAACACATATAAGAAAACATGAAATACAAATTTTATTTTTATTTAATCTTTTGTTTTCAAAATTTTGTTATTAGATTTTCTATCAAACAGGTCGTATACACTACCTTTACAGATAAACTTCTTTGTTTTATTATTTACTTCTTAGAAATGTATTTAGTTTTTAAAAATATTTTTTTAAAATTTAACTAAAAGATTCAAATGTTTTAAGAAAGATTAAAAAGTAAAGAATTGGAATAAAACATAGTACATAAGTTTAAAAACAAAAAAACAATTTCTATGGTTAATATTGAGTTTGGAATAAATTACTAAAATTATAATTTTTTAAAATTTTCTATAAATACCTTTAAAAAATTTATAATTTAAATTGGGTTTTAGTAAAGTTTATTGAATGATTAAAATTCACAGAAGTGTTTTTTTTTAAATTAGAAAAACATAATTTATTACATTAAAAAAAATAAAAAAAAGCACTTTGGAGTTAAATTTAAAAGTCATATTTTTTTAATAATATATTTCGTAGTAGTTTCTTCAAAATTGACTTTTAAATTAATCCAAAATTTCTAAAAGTATTGTTTATCTATTAAGATTAATAAAAACCCTTTTGATAATCTAAAAAAATTAACAAAAAAACTACACTACTAAACAAACTTTCAATTATTAATTGTTTTTCAATTTTAGTTGCTAGTAGAAGAAAAGCACAAGGAACATAAGTTCATACTAGAAGCATATCGTTCAAAAGCAGAGCATTACCTTTGTAGTTGTCTTAACAAAAACAAGAACAACTCAACTAATCTGAAACGCACCCCAGGAGGTTTACTATACACCCGCAAATGGAACAATCTCCAATACGTCACCACCGCAGCTTTTCTTCTCACCCTTTACTCCGACCATCTCCAAGCCTCAAATCAACGGCTCAAATGCAATCAACTTGAAGTGGGACCTCAAGAGATGTTGACTTTGGCTAAATCTCAAGTTGACTACATCTTGGGTGCTAATCCAATGGCCATGAGTTACTTGGTCGGGTTTGGTCCACAGTACCCTCAAAGGGTACACCACAGAGGAGCGTCGATGGAATCAATCAAAGATAATGAGGGATTTATCGGGTGCGTGCAAGGGTATGATAATTGGTATGGTCGTGTTGATCGAAATCCTAATGTGTTGATGGGTGCTGTGGTGGGTGGGCTCAATGAGAAAGATGAATTTGTTGATGAACGATCTAATTATATGCAGACTGAAGCTTGCACTTACAATACAGCGCCGTTGGTTGGTGTATTTGCTAAATTGAATCAATTAGAATCTCAAAGCAACAAAATTAGACCTCCACTAAATGTTGATTCTCATCATAATAATCGTATGTAA

mRNA sequence

ATGAAGGCATCAAAAGCAATTGCCTTTCTCATCTTCTTTCTTCTCATCCTTCCTTTAACCCATTCCTTGTTCGACTATCACCAAGCTCTCTCCATTAGTCTCCTCTACTTTGAGTCTCAACGCTCCGGTCACCTCCCCTACAACCAACGACTCACTTGGCGCCACCATTCCGGCCTCTCTGATGGCCTCCGTCAAGGAGTGGACTTAGTGGGAGGGTACTATGATGCCGGCGACAATGTGAAGTTCGGTTTACCAATGGCATTCACCATCACAATGTTGTCGTGGGGTGTCATTGAATATGGAGAACAAATTGCAGCCGCCGAAGAGTACACTCATGCGCTAGAGGCTATCAAGTGGGGTACTGATTACTTCATCAAAGCTCACCCGCACCCAAACGTCTTCTGGGCACAGGTGGGTGACGGGGCAACGGATCACTACTGCTGGCAGCGGCCGGAGGACATGACAACGTCTCGACGAGCCTACAAGATCGACGAAAACAACCCGGGTTCCGATGTTGCCGGAGAGACGGCGGCGGCAATGGCAGCTGCAGCAATTGTTTTCAGTAGAACAAACCCACATTACTCGCATCTACTACTCCACCATGCACAAGAGGCAAAGCATTACCTTTGTAGTTGTCTTAACAAAAACAAGAACAACTCAACTAATCTGAAACGCACCCCAGGAGGTTTACTATACACCCGCAAATGGAACAATCTCCAATACGTCACCACCGCAGCTTTTCTTCTCACCCTTTACTCCGACCATCTCCAAGCCTCAAATCAACGGCTCAAATGCAATCAACTTGAAGTGGGACCTCAAGAGATGTTGACTTTGGCTAAATCTCAAGTTGACTACATCTTGGGTGCTAATCCAATGGCCATGAGTTACTTGGTCGGGTTTGGTCCACAGTACCCTCAAAGGGTACACCACAGAGGAGCGTCGATGGAATCAATCAAAGATAATGAGGGATTTATCGGGTGCGTGCAAGGGTATGATAATTGGTATGGTCGTGTTGATCGAAATCCTAATGTGTTGATGGGTGCTGTGGTGGGTGGGCTCAATGAGAAAGATGAATTTGTTGATGAACGATCTAATTATATGCAGACTGAAGCTTGCACTTACAATACAGCGCCGTTGGTTGGTGTATTTGCTAAATTGAATCAATTAGAATCTCAAAGCAACAAAATTAGACCTCCACTAAATGTTGATTCTCATCATAATAATCGTATGTAA

Coding sequence (CDS)

ATGAAGGCATCAAAAGCAATTGCCTTTCTCATCTTCTTTCTTCTCATCCTTCCTTTAACCCATTCCTTGTTCGACTATCACCAAGCTCTCTCCATTAGTCTCCTCTACTTTGAGTCTCAACGCTCCGGTCACCTCCCCTACAACCAACGACTCACTTGGCGCCACCATTCCGGCCTCTCTGATGGCCTCCGTCAAGGAGTGGACTTAGTGGGAGGGTACTATGATGCCGGCGACAATGTGAAGTTCGGTTTACCAATGGCATTCACCATCACAATGTTGTCGTGGGGTGTCATTGAATATGGAGAACAAATTGCAGCCGCCGAAGAGTACACTCATGCGCTAGAGGCTATCAAGTGGGGTACTGATTACTTCATCAAAGCTCACCCGCACCCAAACGTCTTCTGGGCACAGGTGGGTGACGGGGCAACGGATCACTACTGCTGGCAGCGGCCGGAGGACATGACAACGTCTCGACGAGCCTACAAGATCGACGAAAACAACCCGGGTTCCGATGTTGCCGGAGAGACGGCGGCGGCAATGGCAGCTGCAGCAATTGTTTTCAGTAGAACAAACCCACATTACTCGCATCTACTACTCCACCATGCACAAGAGGCAAAGCATTACCTTTGTAGTTGTCTTAACAAAAACAAGAACAACTCAACTAATCTGAAACGCACCCCAGGAGGTTTACTATACACCCGCAAATGGAACAATCTCCAATACGTCACCACCGCAGCTTTTCTTCTCACCCTTTACTCCGACCATCTCCAAGCCTCAAATCAACGGCTCAAATGCAATCAACTTGAAGTGGGACCTCAAGAGATGTTGACTTTGGCTAAATCTCAAGTTGACTACATCTTGGGTGCTAATCCAATGGCCATGAGTTACTTGGTCGGGTTTGGTCCACAGTACCCTCAAAGGGTACACCACAGAGGAGCGTCGATGGAATCAATCAAAGATAATGAGGGATTTATCGGGTGCGTGCAAGGGTATGATAATTGGTATGGTCGTGTTGATCGAAATCCTAATGTGTTGATGGGTGCTGTGGTGGGTGGGCTCAATGAGAAAGATGAATTTGTTGATGAACGATCTAATTATATGCAGACTGAAGCTTGCACTTACAATACAGCGCCGTTGGTTGGTGTATTTGCTAAATTGAATCAATTAGAATCTCAAAGCAACAAAATTAGACCTCCACTAAATGTTGATTCTCATCATAATAATCGTATGTAA

Protein sequence

MKASKAIAFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQEAKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLKCNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDNWYGRVDRNPNVLMGAVVGGLNEKDEFVDERSNYMQTEACTYNTAPLVGVFAKLNQLESQSNKIRPPLNVDSHHNNRM
Homology
BLAST of Lsi05G005740 vs. ExPASy Swiss-Prot
Match: O48766 (Endoglucanase 11 OS=Arabidopsis thaliana OX=3702 GN=At2g32990 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.3e-155
Identity = 277/474 (58.44%), Postives = 322/474 (67.93%), Query Frame = 0

Query: 24  FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFG 83
           FDY +ALS SLLYFE+QRSG LPYNQR+TWR HSGL+DGL QGVDLVGGY+DAGD+VKFG
Sbjct: 38  FDYGEALSKSLLYFEAQRSGRLPYNQRVTWRDHSGLTDGLEQGVDLVGGYHDAGDHVKFG 97

Query: 84  LPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGAT 143
           LPMAFT+TMLSW VIEYG+ +A+  E +HALEAIKWGTDYFIKAH  PNV WA+VGDG T
Sbjct: 98  LPMAFTVTMLSWSVIEYGDSLASTGELSHALEAIKWGTDYFIKAHTSPNVLWAEVGDGDT 157

Query: 144 DHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQ 203
           DHYCWQRPEDMTTSRRA+KIDENNPGSD+AGETAAAMAAA+IVF  TNPHYSHLLLHHAQ
Sbjct: 158 DHYCWQRPEDMTTSRRAFKIDENNPGSDIAGETAAAMAAASIVFRSTNPHYSHLLLHHAQ 217

Query: 204 E----------------------------------------------------------- 263
           +                                                           
Sbjct: 218 QLFEFGDKYRGKYDESLKVVKSYYASVSGYMDELLWGATWLYRATDNEHYMSYVVDMAHQ 277

Query: 264 ----------------------------------------------AKHYLCSCLNKNKN 323
                                                         A HYLCS LNKN  
Sbjct: 278 LGGLSWAMSEFSWDVKFAGVQLLASMLLKEEKHKQHSKVLQQYKSKADHYLCSILNKN-I 337

Query: 324 NSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLKCNQLEVGPQEMLTL 383
           N TN++RTP GLLY R+WNN+QYV+TA+FLLT+YSDHL+ SN  L+C++  V P EML  
Sbjct: 338 NGTNVQRTPAGLLYVRQWNNMQYVSTASFLLTVYSDHLRKSNTDLECHEGTVTPDEMLGF 397

Query: 384 AKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDNWYGRV 393
           AKSQ+DYILG+NPM  SYLVG+GP+YP RVHHRGAS+ S K+++GFIGC QGYDNWYGR 
Sbjct: 398 AKSQIDYILGSNPMETSYLVGYGPKYPIRVHHRGASIASFKEHKGFIGCTQGYDNWYGRS 457

BLAST of Lsi05G005740 vs. ExPASy Swiss-Prot
Match: Q6H3Z9 (Endoglucanase 22 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU11 PE=2 SV=2)

HSP 1 Score: 503.8 bits (1296), Expect = 1.8e-141
Identity = 251/476 (52.73%), Postives = 312/476 (65.55%), Query Frame = 0

Query: 25  DYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFGL 84
           DY +AL  SLLYFE+QRSG LPY+QR+TWR HSGL+DGL+QGVDLVGGYYDAGD+VKFGL
Sbjct: 55  DYREALEKSLLYFEAQRSGRLPYSQRVTWRGHSGLTDGLQQGVDLVGGYYDAGDHVKFGL 114

Query: 85  PMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATD 144
           PMAFT+TMLSWG I++   IAAA E+ HALEAIKWGTDYF+KAH HP V+WA+VGDG TD
Sbjct: 115 PMAFTVTMLSWGAIDFAADIAAAGEWRHALEAIKWGTDYFVKAHTHPFVYWAEVGDGDTD 174

Query: 145 HYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQE 204
           HYCWQRPEDMTTSR+AY++D +NPGSD+AGETAAA+AAA+IVF R++PHYSHLLLHHAQ+
Sbjct: 175 HYCWQRPEDMTTSRQAYRVDRDNPGSDLAGETAAALAAASIVFRRSDPHYSHLLLHHAQQ 234

Query: 205 ------------------------------------------------------------ 264
                                                                       
Sbjct: 235 LFEFGDTYRGSYDSSIEEVRSYYASVSGYHDELLWAALWLHRATGKEEYLRYAVDNADSF 294

Query: 265 -----------------------------------------------AKHYLCSCLNKNK 324
                                                          A+HYLC+CL +N 
Sbjct: 295 GGVGWAITEFSWDVKYAGLQVLAAKLLLDGDPQAAAHRGVLEKYREKAEHYLCACLGRNI 354

Query: 325 NNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQAS----NQRLKCNQLEVGPQ 384
           N + N+ R+PGG+LY R+WNNLQY ++AAFLLT YS +L +S    +  L+C        
Sbjct: 355 NGADNVDRSPGGMLYVRQWNNLQYASSAAFLLTAYSHYLSSSSASASAALRCPGGAAAAA 414

Query: 385 EMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDN 390
           EM++LA+SQ DYILG NP+ +SY+VG+G +YP RVHHRGAS+ S K++  FIGCVQG+D+
Sbjct: 415 EMVSLARSQADYILGRNPLRLSYMVGYGRRYPARVHHRGASIVSHKEDGRFIGCVQGFDD 474

BLAST of Lsi05G005740 vs. ExPASy Swiss-Prot
Match: Q6Z5P2 (Endoglucanase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU10 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 1.6e-134
Identity = 244/481 (50.73%), Postives = 299/481 (62.16%), Query Frame = 0

Query: 25  DYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFGL 84
           +Y  AL  SLLYFE+QRSG LP+NQR+ WR HSGL+DGL QGVDLVGGYYDAGD+VKFGL
Sbjct: 36  NYEDALRKSLLYFEAQRSGRLPHNQRVAWRDHSGLTDGLEQGVDLVGGYYDAGDHVKFGL 95

Query: 85  PMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATD 144
           PMAFT+TMLSW +IEYG+ + AA E  HALEAIKWGTDYFIKAH  PN  WA+VGDG TD
Sbjct: 96  PMAFTVTMLSWSMIEYGDDVEAAGELGHALEAIKWGTDYFIKAHTKPNELWAEVGDGDTD 155

Query: 145 HYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQE 204
           HYCWQRPEDMTTSR+AYK+D   PGSDVAGETAAAMAAA+IVF ++NPHY+ LLLHHAQ+
Sbjct: 156 HYCWQRPEDMTTSRQAYKVDRERPGSDVAGETAAAMAAASIVFRKSNPHYASLLLHHAQQ 215

Query: 205 ------------------------------------------------------------ 264
                                                                       
Sbjct: 216 LFEFADKYRGKYDSSIAEVKSYYASVSGYKDELLWAALWLHRATGKAHYLDYVVDNADCF 275

Query: 265 ----------------------------------------------AKHYLCSCLNKNKN 324
                                                         A+HY+C CL +N +
Sbjct: 276 GGTGWAITEFSWDVKYAGVQILAARLLLRGEHEERHRSTLEQYRAKAEHYVCGCLGRNAD 335

Query: 325 N--STNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHL-QASNQRLKCNQLE-VGPQE 384
                N++R+PGG+LY R+WNN+QYVT AAFLL  Y+D+L   ++  + C   E  G  E
Sbjct: 336 GGADANVERSPGGMLYVRQWNNMQYVTNAAFLLAAYADYLGDDADGAVSCAGGETAGAGE 395

Query: 385 MLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDNW 396
           +  LA++QVDY+LG NP  +SYLVG+G +YP RVHHR AS+   K ++ FIGC QG+D+W
Sbjct: 396 VAALARAQVDYVLGTNPRGISYLVGYGAKYPNRVHHRAASIVPYKHSKEFIGCTQGFDHW 455

BLAST of Lsi05G005740 vs. ExPASy Swiss-Prot
Match: Q654U4 (Endoglucanase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0247900 PE=2 SV=2)

HSP 1 Score: 472.6 bits (1215), Expect = 4.4e-132
Identity = 244/506 (48.22%), Postives = 303/506 (59.88%), Query Frame = 0

Query: 6   AIAFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQ 65
           A+A L+          +  DY +AL  SLLYFE+QRSG LP+ QR+ WR HSGL+DGL Q
Sbjct: 11  AVALLLGAAAAAAAAAARHDYEEALRKSLLYFEAQRSGRLPHGQRVAWRDHSGLTDGLEQ 70

Query: 66  GVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFI 125
           GVDLVGGYYDAGD+VKFGLPMAFT+TMLSW ++EYG  +AAA E  HAL+AIKWGTDYFI
Sbjct: 71  GVDLVGGYYDAGDHVKFGLPMAFTVTMLSWSLLEYGADVAAAGELAHALDAIKWGTDYFI 130

Query: 126 KAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAI 185
           KAH  P+  WA+VGDG TDHYCWQRPEDMTTSR+AYK+D   PGSDVAGETAAAMAAA+I
Sbjct: 131 KAHTKPHELWAEVGDGDTDHYCWQRPEDMTTSRQAYKVDRRRPGSDVAGETAAAMAAASI 190

Query: 186 VFSRTNPHYSHLLLHHAQE----------------------------------------- 245
           VF ++NPHYSHLLLHHAQ+                                         
Sbjct: 191 VFRQSNPHYSHLLLHHAQQLFEFADTYRGKYDSSIAEVKSYYASVSGYHDELLWAALWLH 250

Query: 246 ------------------------------------------------------------ 305
                                                                       
Sbjct: 251 RATGRAAYLDYAVDNADEFGGTGWAITEFSWDVKYAGVQILAARLLMRGEHEERHRGTLE 310

Query: 306 -----AKHYLCSCLNKNK--NNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQ 365
                A+HY+C+C+ +N       N++R+PGG+LY R+WNN+QYVT AAFLL+ YSD+L 
Sbjct: 311 RYREKAEHYVCACMGRNAAGGADANVERSPGGMLYVRQWNNMQYVTNAAFLLSAYSDYLA 370

Query: 366 ASNQ---------RLKCNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRV 395
            +                    G  E+   A+ QVDY+LG+NP  MSYLVG+G ++P RV
Sbjct: 371 GAGDGDGDGGGGVATCVGGGGAGAGEVFAAAREQVDYVLGSNPRGMSYLVGYGERFPARV 430

BLAST of Lsi05G005740 vs. ExPASy Swiss-Prot
Match: Q67UW5 (Endoglucanase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0151300 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 9.3e-130
Identity = 251/502 (50.00%), Postives = 295/502 (58.76%), Query Frame = 0

Query: 8   AFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGV 67
           A ++   L +    +  DY +ALS SLLYFE+QRSG LPYNQR+ WR HSGL+DGL QGV
Sbjct: 12  AAVVAVSLAMAAAAAAHDYGEALSKSLLYFEAQRSGRLPYNQRVRWRGHSGLTDGLEQGV 71

Query: 68  DLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKA 127
           DLVGGYYDAGD+VKFGLPMAFT+TMLSW V+EYGE+IAAA E  HAL AIKWGTDYFIKA
Sbjct: 72  DLVGGYYDAGDHVKFGLPMAFTVTMLSWSVLEYGEEIAAAGELGHALHAIKWGTDYFIKA 131

Query: 128 HPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVF 187
           H HPNV W QVGDG +DHYCWQRPEDMTTSR AYK+D  NPGS+VA ETAAAMAAA+IVF
Sbjct: 132 HTHPNVLWTQVGDGDSDHYCWQRPEDMTTSRHAYKVDAENPGSEVAAETAAAMAAASIVF 191

Query: 188 SRT-NPHYSHLLLHHAQE------------------------------------------ 247
            R  + HY+HLLLHHAQ+                                          
Sbjct: 192 RRAGDAHYAHLLLHHAQQLFEFGDKYRGRYDESVEVVKNYYPSSSGYKDELLWAALWLHR 251

Query: 248 ------------------------------------------------------------ 307
                                                                       
Sbjct: 252 ATGRREYLDYAVDNADDFGGTGWAVSEFSWDIKYAGLQVLASKLLVEEKHLSSQQREVLE 311

Query: 308 -----AKHYLCSCLNKNKNNST-NLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQA 367
                A++Y+CSC+ +N   +  N  RTP GLL+ R WNNLQYV+ AAFLLT+YSD L  
Sbjct: 312 KYRSKAEYYVCSCMGRNPGGAAHNAGRTPAGLLFIRPWNNLQYVSNAAFLLTVYSDVLSY 371

Query: 368 SNQRLKCNQLEVGPQ----------EMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRV 391
            +  L C   +              E+L  A+SQ DYILG NPMA SYLVG+G  YP+RV
Sbjct: 372 LSLPLLCPDPDAAADEAAPAAADAGEVLEFARSQADYILGTNPMATSYLVGYGEAYPRRV 431

BLAST of Lsi05G005740 vs. ExPASy TrEMBL
Match: A0A6J1F1P0 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111438604 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.3e-187
Identity = 339/484 (70.04%), Postives = 354/484 (73.14%), Query Frame = 0

Query: 11  IFFLLILPLTHSL-FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDL 70
           I FLLILP THSL  DYH ALSI+LLYFESQRSG LPYNQRL WRHHS LSDGL QGVDL
Sbjct: 6   ISFLLILPFTHSLSHDYHHALSITLLYFESQRSGRLPYNQRLPWRHHSALSDGLHQGVDL 65

Query: 71  VGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHP 130
           VGGYYDAGDN+KFGLPMAFT+TMLSWGVIEYGEQ+A A EYTHALEAIKWGTDYFIKAHP
Sbjct: 66  VGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLATAGEYTHALEAIKWGTDYFIKAHP 125

Query: 131 HPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSR 190
           HPNVFWAQVGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMAAAAIVFSR
Sbjct: 126 HPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSR 185

Query: 191 TNPHYSHLLLHHAQE--------------------------------------------- 250
           TNPHYSH+LLHHAQE                                             
Sbjct: 186 TNPHYSHVLLHHAQELFEFGDKNRGKYDESIKEAKGYYSSVSGYKDELLWAAMWLYKSSG 245

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 246 REEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAK 305

Query: 311 AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLK 370
           A+HYLC CLNKNKNN TNLKRTPGGLLYTRKWNNLQYV+TAAFLLT+YSDHLQASNQRL 
Sbjct: 306 AEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTVYSDHLQASNQRLT 365

Query: 371 CNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGF 389
           C+Q EVGPQEMLT AKSQVDYILG NPMAMSYLVGFGP+YPQRVHHRGAS+ESIK N GF
Sbjct: 366 CHQHEVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGF 425

BLAST of Lsi05G005740 vs. ExPASy TrEMBL
Match: A0A5A7UIW1 (Cellulase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold104G00290 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 2.1e-185
Identity = 341/498 (68.47%), Postives = 367/498 (73.69%), Query Frame = 0

Query: 3   ASKAIAFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDG 62
           ++KAIA +I FL ILPLTHS F+YHQALSISLLYFESQRSG LPYNQRLTWRHHSGL+DG
Sbjct: 4   SNKAIASIISFLSILPLTHS-FNYHQALSISLLYFESQRSGRLPYNQRLTWRHHSGLADG 63

Query: 63  LRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTD 122
           L QGVDLVGGYYDAGDNVKFGLPMAFT+TMLSWGVIEYGEQIAAA EYTHALEAIKWGTD
Sbjct: 64  LHQGVDLVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYTHALEAIKWGTD 123

Query: 123 YFIKAHPHPNVFWAQ-VGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMA 182
           YFIKAHPHPNV WAQ VGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMA
Sbjct: 124 YFIKAHPHPNVLWAQVVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMA 183

Query: 183 AAAIVFSRTNPHYSHLLLHHAQE------------------------------------- 242
           AAAIVFSRTNPHYS LLLHHA+E                                     
Sbjct: 184 AAAIVFSRTNPHYSQLLLHHAEELFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAA 243

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 244 MWLHKASGKEEYLKYVIENALDFGGISWAINEFSWDIKYAGLQILASMLVVEEKQKEEKL 303

Query: 303 --------AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHL 362
                   A+HYLCSCLNK+  N+ NLKRTPGGLLYTRKWNNLQYV TA FLLTLYSDHL
Sbjct: 304 IVEAYKSKAEHYLCSCLNKSSTNN-NLKRTPGGLLYTRKWNNLQYVATATFLLTLYSDHL 363

Query: 363 QASNQRLKC-NQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASM 394
           Q+SNQRL+C N  EVG +EML+LAKSQVDYI+G NPM MSYLVGFGP+YP+RVHHRGASM
Sbjct: 364 QSSNQRLRCSNDDEVGAEEMLSLAKSQVDYIVGDNPMGMSYLVGFGPRYPERVHHRGASM 423

BLAST of Lsi05G005740 vs. ExPASy TrEMBL
Match: A0A6J1L827 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111500164 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.2e-183
Identity = 336/484 (69.42%), Postives = 353/484 (72.93%), Query Frame = 0

Query: 11  IFFLLILPLTHSL-FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDL 70
           I FLLILP THSL  DYH ALSI+LLYFESQRSG LPYNQRL WRHHS LSDGL QGVDL
Sbjct: 6   ISFLLILPFTHSLSHDYHHALSITLLYFESQRSGRLPYNQRLPWRHHSALSDGLHQGVDL 65

Query: 71  VGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHP 130
           VGGYYDAGDN+KFGLPMAFT+TMLSWGVIEYGEQ+AAA EYTHALEAIKWGTDYFIKAHP
Sbjct: 66  VGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYTHALEAIKWGTDYFIKAHP 125

Query: 131 HPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSR 190
           HPNVFWAQVGDG TDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMAAAAIVFSR
Sbjct: 126 HPNVFWAQVGDGVTDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSR 185

Query: 191 TNPHYSHLLLHHAQE--------------------------------------------- 250
           TNPHYSH+LLHHAQE                                             
Sbjct: 186 TNPHYSHVLLHHAQELFEFGDKHRGKYDESIEEAKGYYPSVSGYKDELLWAAMWLYKSSG 245

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 246 REEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLSVEEQSKEHKSIMEAYRAK 305

Query: 311 AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLK 370
           A++YLC CL  NK+NSTNLKRTP GLLYTRKWNNLQ+V+TAAFLLT+YSDHLQASNQRL 
Sbjct: 306 AEYYLCGCL--NKSNSTNLKRTPEGLLYTRKWNNLQFVSTAAFLLTVYSDHLQASNQRLT 365

Query: 371 CNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGF 389
           C+Q EVGPQEMLT AKSQVDYILG NPMAMSYLVGFGP+YPQRVHHRGAS+ESIK N GF
Sbjct: 366 CHQHEVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGF 425

BLAST of Lsi05G005740 vs. ExPASy TrEMBL
Match: A0A5D3DKF6 (Cellulase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00470 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 5.7e-183
Identity = 328/445 (73.71%), Postives = 360/445 (80.90%), Query Frame = 0

Query: 3   ASKAIAFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDG 62
           ++KAIA +I FL ILPLTHS F+YHQALSISLLYFESQRSG LPYNQRLTWRHHSGL+DG
Sbjct: 4   SNKAIASIISFLSILPLTHS-FNYHQALSISLLYFESQRSGRLPYNQRLTWRHHSGLADG 63

Query: 63  LRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTD 122
           L QGVDLVGGYYDAGDNVKFGLPMAFT+TMLSWGVIEYGEQIAAA EYTHALEAIKWGTD
Sbjct: 64  LHQGVDLVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYTHALEAIKWGTD 123

Query: 123 YFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAA 182
           YFIKAHPHPNV WAQVGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMAA
Sbjct: 124 YFIKAHPHPNVLWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAA 183

Query: 183 AAIVFSRTNPH--------------------------YSHLLLHHA-------------- 242
           AAI+F   + +                          ++ + LH A              
Sbjct: 184 AAILFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAAMWLHKASGKEEYLKYLVVEE 243

Query: 243 -------------QEAKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLL 302
                         +A+HYLCSCLNK+  N+ NLKRTPGGLLYTRKWNNLQYV TA FLL
Sbjct: 244 KQKEEKLIVEAYKSKAEHYLCSCLNKSSTNN-NLKRTPGGLLYTRKWNNLQYVATATFLL 303

Query: 303 TLYSDHLQASNQRLKC-NQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRV 362
           TLYSDHLQ+SNQRL+C N  EVG +EML+LAKSQVDYI+G NPM MSYLVGFGP+YP+RV
Sbjct: 304 TLYSDHLQSSNQRLRCSNDDEVGAEEMLSLAKSQVDYIVGDNPMGMSYLVGFGPRYPERV 363

Query: 363 HHRGASMESIKDNEGFIGCVQGYDNWYGRVDRNPNVLMGAVVGGLNEKDEFVDERSNYMQ 394
           HHRGASMES+K+  GFIGCVQGYD+WYGRVDRNPNVLMGAVVGG NE+DEF DERSN+MQ
Sbjct: 364 HHRGASMESVKEKRGFIGCVQGYDDWYGRVDRNPNVLMGAVVGGPNERDEFNDERSNFMQ 423

BLAST of Lsi05G005740 vs. ExPASy TrEMBL
Match: A0A0A0L504 (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G073940 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.7e-177
Identity = 325/470 (69.15%), Postives = 357/470 (75.96%), Query Frame = 0

Query: 3   ASKAIA-FLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSD 62
           ++KA A  +IFFLLILPLTHS F+Y QALSISLLYFESQRSG LPYNQRLTWRHHSGL+D
Sbjct: 4   SNKAFASTMIFFLLILPLTHS-FNYQQALSISLLYFESQRSGRLPYNQRLTWRHHSGLTD 63

Query: 63  GLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGT 122
           GL QGVDLVGGYYDAGDNVKFGLPMAFT+TMLSWGVIEYGEQIAAA EY HALEAIKWGT
Sbjct: 64  GLHQGVDLVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYIHALEAIKWGT 123

Query: 123 DYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMA 182
           DYFIKAHPHPNV WAQVGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMA
Sbjct: 124 DYFIKAHPHPNVLWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMA 183

Query: 183 AAAIVF----------------------------------------SRTNPHYSHLLLHH 242
           AAAI+F                                        +     Y   ++ +
Sbjct: 184 AAAILFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAATWLYKASGKEEYLKYVIEN 243

Query: 243 A------------------------------------QEAKHYLCSCLNKNKNNSTNLKR 302
           A                                     +A+HYLCSCLNK+  N+ NLKR
Sbjct: 244 ALDFGGISWAINEFSWDIKYAVEEKQKEEKLIVEAYKSKAEHYLCSCLNKSSTNN-NLKR 303

Query: 303 TPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLKC-NQLEVGPQEMLTLAKSQVD 362
           TPGGLLYTRKWNNLQYVTTA FLLTLYSDHL++SNQRL+C N  EVGP+EML+LAKSQVD
Sbjct: 304 TPGGLLYTRKWNNLQYVTTATFLLTLYSDHLESSNQRLRCSNDDEVGPEEMLSLAKSQVD 363

Query: 363 YILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDNWYGRVDRNPNV 395
           YILG NPM MSYLVGFG +YP+RVHHRGASMES+K+  GFIGCVQGYD+WYGRVD NPNV
Sbjct: 364 YILGENPMGMSYLVGFGGRYPERVHHRGASMESVKEKRGFIGCVQGYDDWYGRVDGNPNV 423

BLAST of Lsi05G005740 vs. NCBI nr
Match: XP_038904226.1 (endoglucanase 11-like [Benincasa hispida])

HSP 1 Score: 706.4 bits (1822), Expect = 1.4e-199
Identity = 366/503 (72.76%), Postives = 377/503 (74.95%), Query Frame = 0

Query: 1   MKASKAIAFLIF-FLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGL 60
           MKASKA A LI  FLLILPL HS FDYH ALSISLLYFESQRSG LPYNQRLTWRHHSGL
Sbjct: 1   MKASKASASLIVSFLLILPLIHS-FDYHHALSISLLYFESQRSGRLPYNQRLTWRHHSGL 60

Query: 61  SDGLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKW 120
           +DGLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQ+AAA EYTHALEAIKW
Sbjct: 61  ADGLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQMAAAGEYTHALEAIKW 120

Query: 121 GTDYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAA 180
           GTDYFIKAH HPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGET AA
Sbjct: 121 GTDYFIKAHSHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETTAA 180

Query: 181 MAAAAIVFSRTNPHYSHLLLHHAQE----------------------------------- 240
           MAAAAIVFSRTNPHYSHLLLHHAQE                                   
Sbjct: 181 MAAAAIVFSRTNPHYSHLLLHHAQELFEFGDKYRGKYDESIEVVKGYYSSVSGYMDELLW 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AAMWLYKASGREEYLKYVLENGLNFGGISWAINEFSWDVKYAGLQILASMLLAEEKHKEH 300

Query: 301 ----------AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSD 360
                     A+HYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLT+YSD
Sbjct: 301 KFILEAYHSKAEHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTVYSD 360

Query: 361 HLQASNQRLKCNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGAS 398
           HL+ASNQRLKC+Q EVGPQEMLTLAKSQVDYILGANPMA SYLVGFGP+YPQRVHHRGAS
Sbjct: 361 HLRASNQRLKCDQHEVGPQEMLTLAKSQVDYILGANPMATSYLVGFGPRYPQRVHHRGAS 420

BLAST of Lsi05G005740 vs. NCBI nr
Match: XP_022932240.1 (endoglucanase 11-like [Cucurbita moschata])

HSP 1 Score: 664.8 bits (1714), Expect = 4.7e-187
Identity = 339/484 (70.04%), Postives = 354/484 (73.14%), Query Frame = 0

Query: 11  IFFLLILPLTHSL-FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDL 70
           I FLLILP THSL  DYH ALSI+LLYFESQRSG LPYNQRL WRHHS LSDGL QGVDL
Sbjct: 6   ISFLLILPFTHSLSHDYHHALSITLLYFESQRSGRLPYNQRLPWRHHSALSDGLHQGVDL 65

Query: 71  VGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHP 130
           VGGYYDAGDN+KFGLPMAFT+TMLSWGVIEYGEQ+A A EYTHALEAIKWGTDYFIKAHP
Sbjct: 66  VGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLATAGEYTHALEAIKWGTDYFIKAHP 125

Query: 131 HPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSR 190
           HPNVFWAQVGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMAAAAIVFSR
Sbjct: 126 HPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSR 185

Query: 191 TNPHYSHLLLHHAQE--------------------------------------------- 250
           TNPHYSH+LLHHAQE                                             
Sbjct: 186 TNPHYSHVLLHHAQELFEFGDKNRGKYDESIKEAKGYYSSVSGYKDELLWAAMWLYKSSG 245

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 246 REEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAK 305

Query: 311 AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLK 370
           A+HYLC CLNKNKNN TNLKRTPGGLLYTRKWNNLQYV+TAAFLLT+YSDHLQASNQRL 
Sbjct: 306 AEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTVYSDHLQASNQRLT 365

Query: 371 CNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGF 389
           C+Q EVGPQEMLT AKSQVDYILG NPMAMSYLVGFGP+YPQRVHHRGAS+ESIK N GF
Sbjct: 366 CHQHEVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGF 425

BLAST of Lsi05G005740 vs. NCBI nr
Match: KAG7015533.1 (Endoglucanase 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 663.7 bits (1711), Expect = 1.0e-186
Identity = 340/485 (70.10%), Postives = 354/485 (72.99%), Query Frame = 0

Query: 10  LIFFLLILPLTHSL-FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVD 69
           LI FLLILP THSL  DYH ALSI+LLYFESQ SG LPYNQRL WRHHS LSDGL QGVD
Sbjct: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64

Query: 70  LVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAH 129
           LVGGYYDAGDN+KFGLPMAFT+TMLSWGVIEYGEQ+AAA EY HALEAIKWGTDYFIKAH
Sbjct: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124

Query: 130 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFS 189
           PHPNVFWAQVGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMAAAAIVFS
Sbjct: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184

Query: 190 RTNPHYSHLLLHHAQE-------------------------------------------- 249
           RTNPHYSH+LLHHAQE                                            
Sbjct: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244

Query: 250 ------------------------------------------------------------ 309
                                                                       
Sbjct: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304

Query: 310 -AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRL 369
            A+HYLC CLNKNKNN TNLKRTPGGLLYTRKWNNLQYV+TAAFLLTLYSDHLQASNQRL
Sbjct: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364

Query: 370 KCNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEG 389
            C+Q +VGPQEMLT AKSQVDYILG NPMAMSYLVGFGP+YPQRVHHRGAS+ESIK N G
Sbjct: 365 TCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGG 424

BLAST of Lsi05G005740 vs. NCBI nr
Match: KAA0055853.1 (endoglucanase 11-like [Cucumis melo var. makuwa])

HSP 1 Score: 658.3 bits (1697), Expect = 4.4e-185
Identity = 341/498 (68.47%), Postives = 367/498 (73.69%), Query Frame = 0

Query: 3   ASKAIAFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDG 62
           ++KAIA +I FL ILPLTHS F+YHQALSISLLYFESQRSG LPYNQRLTWRHHSGL+DG
Sbjct: 4   SNKAIASIISFLSILPLTHS-FNYHQALSISLLYFESQRSGRLPYNQRLTWRHHSGLADG 63

Query: 63  LRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTD 122
           L QGVDLVGGYYDAGDNVKFGLPMAFT+TMLSWGVIEYGEQIAAA EYTHALEAIKWGTD
Sbjct: 64  LHQGVDLVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYTHALEAIKWGTD 123

Query: 123 YFIKAHPHPNVFWAQ-VGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMA 182
           YFIKAHPHPNV WAQ VGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMA
Sbjct: 124 YFIKAHPHPNVLWAQVVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMA 183

Query: 183 AAAIVFSRTNPHYSHLLLHHAQE------------------------------------- 242
           AAAIVFSRTNPHYS LLLHHA+E                                     
Sbjct: 184 AAAIVFSRTNPHYSQLLLHHAEELFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAA 243

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 244 MWLHKASGKEEYLKYVIENALDFGGISWAINEFSWDIKYAGLQILASMLVVEEKQKEEKL 303

Query: 303 --------AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHL 362
                   A+HYLCSCLNK+  N+ NLKRTPGGLLYTRKWNNLQYV TA FLLTLYSDHL
Sbjct: 304 IVEAYKSKAEHYLCSCLNKSSTNN-NLKRTPGGLLYTRKWNNLQYVATATFLLTLYSDHL 363

Query: 363 QASNQRLKC-NQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASM 394
           Q+SNQRL+C N  EVG +EML+LAKSQVDYI+G NPM MSYLVGFGP+YP+RVHHRGASM
Sbjct: 364 QSSNQRLRCSNDDEVGAEEMLSLAKSQVDYIVGDNPMGMSYLVGFGPRYPERVHHRGASM 423

BLAST of Lsi05G005740 vs. NCBI nr
Match: XP_004149358.3 (endoglucanase 11 [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 9.7e-185
Identity = 340/499 (68.14%), Postives = 367/499 (73.55%), Query Frame = 0

Query: 3   ASKAIA-FLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSD 62
           ++KA A  +IFFLLILPLTHS F+Y QALSISLLYFESQRSG LPYNQRLTWRHHSGL+D
Sbjct: 4   SNKAFASTMIFFLLILPLTHS-FNYQQALSISLLYFESQRSGRLPYNQRLTWRHHSGLTD 63

Query: 63  GLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGT 122
           GL QGVDLVGGYYDAGDNVKFGLPMAFT+TMLSWGVIEYGEQIAAA EY HALEAIKWGT
Sbjct: 64  GLHQGVDLVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYIHALEAIKWGT 123

Query: 123 DYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMA 182
           DYFIKAHPHPNV WAQVGDGATDHYCWQRPEDMTTSR+AYKIDENNPGSDVAGETAAAMA
Sbjct: 124 DYFIKAHPHPNVLWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMA 183

Query: 183 AAAIVFSRTNPHYSHLLLHHAQE------------------------------------- 242
           AAAIVFSRTNPHYSHLLLHHA+E                                     
Sbjct: 184 AAAIVFSRTNPHYSHLLLHHAEELFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAA 243

Query: 243 ------------------------------------------------------------ 302
                                                                       
Sbjct: 244 TWLYKASGKEEYLKYVIENALDFGGISWAINEFSWDIKYAGLQILASMLAVEEKQKEEKL 303

Query: 303 --------AKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHL 362
                   A+HYLCSCLNK+  N+ NLKRTPGGLLYTRKWNNLQYVTTA FLLTLYSDHL
Sbjct: 304 IVEAYKSKAEHYLCSCLNKSSTNN-NLKRTPGGLLYTRKWNNLQYVTTATFLLTLYSDHL 363

Query: 363 QASNQRLKC-NQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASM 395
           ++SNQRL+C N  EVGP+EML+LAKSQVDYILG NPM MSYLVGFG +YP+RVHHRGASM
Sbjct: 364 ESSNQRLRCSNDDEVGPEEMLSLAKSQVDYILGENPMGMSYLVGFGGRYPERVHHRGASM 423

BLAST of Lsi05G005740 vs. TAIR 10
Match: AT2G32990.1 (glycosyl hydrolase 9B8 )

HSP 1 Score: 550.8 bits (1418), Expect = 9.1e-157
Identity = 277/474 (58.44%), Postives = 322/474 (67.93%), Query Frame = 0

Query: 24  FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFG 83
           FDY +ALS SLLYFE+QRSG LPYNQR+TWR HSGL+DGL QGVDLVGGY+DAGD+VKFG
Sbjct: 38  FDYGEALSKSLLYFEAQRSGRLPYNQRVTWRDHSGLTDGLEQGVDLVGGYHDAGDHVKFG 97

Query: 84  LPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGAT 143
           LPMAFT+TMLSW VIEYG+ +A+  E +HALEAIKWGTDYFIKAH  PNV WA+VGDG T
Sbjct: 98  LPMAFTVTMLSWSVIEYGDSLASTGELSHALEAIKWGTDYFIKAHTSPNVLWAEVGDGDT 157

Query: 144 DHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQ 203
           DHYCWQRPEDMTTSRRA+KIDENNPGSD+AGETAAAMAAA+IVF  TNPHYSHLLLHHAQ
Sbjct: 158 DHYCWQRPEDMTTSRRAFKIDENNPGSDIAGETAAAMAAASIVFRSTNPHYSHLLLHHAQ 217

Query: 204 E----------------------------------------------------------- 263
           +                                                           
Sbjct: 218 QLFEFGDKYRGKYDESLKVVKSYYASVSGYMDELLWGATWLYRATDNEHYMSYVVDMAHQ 277

Query: 264 ----------------------------------------------AKHYLCSCLNKNKN 323
                                                         A HYLCS LNKN  
Sbjct: 278 LGGLSWAMSEFSWDVKFAGVQLLASMLLKEEKHKQHSKVLQQYKSKADHYLCSILNKN-I 337

Query: 324 NSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLKCNQLEVGPQEMLTL 383
           N TN++RTP GLLY R+WNN+QYV+TA+FLLT+YSDHL+ SN  L+C++  V P EML  
Sbjct: 338 NGTNVQRTPAGLLYVRQWNNMQYVSTASFLLTVYSDHLRKSNTDLECHEGTVTPDEMLGF 397

Query: 384 AKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDNWYGRV 393
           AKSQ+DYILG+NPM  SYLVG+GP+YP RVHHRGAS+ S K+++GFIGC QGYDNWYGR 
Sbjct: 398 AKSQIDYILGSNPMETSYLVGYGPKYPIRVHHRGASIASFKEHKGFIGCTQGYDNWYGRS 457

BLAST of Lsi05G005740 vs. TAIR 10
Match: AT1G64390.1 (glycosyl hydrolase 9C2 )

HSP 1 Score: 446.4 bits (1147), Expect = 2.4e-125
Identity = 234/503 (46.52%), Postives = 301/503 (59.84%), Query Frame = 0

Query: 1   MKASKAIAFLIFFLLILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLS 60
           M+    +A L+  LL  P+  S  DY QALS SLL+FE+QRSG LP NQR+TWR HSGL+
Sbjct: 1   MEKFAPVAALLLLLLCFPVAFSGHDYGQALSKSLLFFEAQRSGVLPRNQRVTWRSHSGLT 60

Query: 61  DGLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWG 120
           DG   GV+LVGGYYDAGDNVKFGLPMAFT+TM++W VIEYG Q+ A  E  ++++AIKWG
Sbjct: 61  DGKSSGVNLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGNQLQANGELGNSIDAIKWG 120

Query: 121 TDYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAM 180
           TDYFIKAHP PNV + +VGDG TDHYCWQRPE+MTT R+AY+ID +NPGSD+AGETAAAM
Sbjct: 121 TDYFIKAHPEPNVLYGEVGDGNTDHYCWQRPEEMTTDRKAYRIDPSNPGSDLAGETAAAM 180

Query: 181 AAAAIVFSRTNPHYSHLLLHHA-------------------------------------- 240
           AAA+IVF R+NP YS LLL HA                                      
Sbjct: 181 AAASIVFRRSNPVYSRLLLTHAYQLFDFADKYRGKYDSSITVAQKYYRSVSGYNDELLWA 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AAWLYQASNNQFYLDYLGRNGDAMGGTGWSMTEFGWDVKYAGVQTLVAKFLMQGKAGRHA 300

Query: 301 -------QEAKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDH 360
                  ++A  ++CS L K   +S N+++TPGGL++ ++WNN+Q+VT+A+FL T+YSD+
Sbjct: 301 PVFRKYQEKADSFMCSLLGK---SSRNIQKTPGGLIFRQRWNNMQFVTSASFLTTVYSDY 360

Query: 361 LQASNQRLKCNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASM 399
           L +S   L+C    V P ++L+ AKSQVDYILG NP A SY+VG+G  +PQRVHHRG+S+
Sbjct: 361 LTSSRSNLRCAAGNVAPSQLLSFAKSQVDYILGDNPRATSYMVGYGNNFPQRVHHRGSSI 420

BLAST of Lsi05G005740 vs. TAIR 10
Match: AT4G11050.1 (glycosyl hydrolase 9C3 )

HSP 1 Score: 438.3 bits (1126), Expect = 6.6e-123
Identity = 229/492 (46.54%), Postives = 294/492 (59.76%), Query Frame = 0

Query: 1   MKASKAIAFLIFFLL-ILPLTHSLFDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGL 60
           M +   I+ L+  LL ++ L  S  DY QALS S+L+FE+QRSGHLP NQR++WR HSGL
Sbjct: 1   MGSRTTISILVVLLLGLVQLAISGHDYKQALSKSILFFEAQRSGHLPPNQRVSWRSHSGL 60

Query: 61  SDGLRQGVDLVGGYYDAGDNVKFGLPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKW 120
            DG   GVDLVGGYYDAGDNVKFGLPMAFT+T + W +IEYG Q+ +  E  HA++A+KW
Sbjct: 61  YDGKSSGVDLVGGYYDAGDNVKFGLPMAFTVTTMCWSIIEYGGQLESNGELGHAIDAVKW 120

Query: 121 GTDYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAA 180
           GTDYFIKAHP PNV + +VGDG +DHYCWQRPE+MTT RRAYKID NNPGSD+AGETAAA
Sbjct: 121 GTDYFIKAHPEPNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNNPGSDLAGETAAA 180

Query: 181 MAAAAIVFSRTNPHYSHLLLHHA------------------------------------- 240
           MAAA+IVF R++P YS  LL HA                                     
Sbjct: 181 MAAASIVFRRSDPSYSAELLRHAHQLFEFADKYRGKYDSSITVAQKYYRSVSGYNDELLW 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AAAWLYQATNDKYYLDYLGKNGDSMGGTGWSMTEFGWDVKYAGVQTLVAKVLMQGKGGEH 300

Query: 301 --------QEAKHYLCSCLNKNKNNSTNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSD 360
                   Q+A+ ++CS L K   ++ N+K+TPGGL++ + WNN+Q+VT+A+FL T+YSD
Sbjct: 301 TAVFERYQQKAEQFMCSLLGK---STKNIKKTPGGLIFRQSWNNMQFVTSASFLATVYSD 360

Query: 361 HLQASNQRLKCNQLEVGPQEMLTLAKSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGAS 387
           +L  S + L C+Q  + P ++L  +KSQVDYILG NP A SY+VG+G  YP++VHHRG+S
Sbjct: 361 YLSYSKRDLLCSQGNISPSQLLEFSKSQVDYILGDNPRATSYMVGYGENYPRQVHHRGSS 420

BLAST of Lsi05G005740 vs. TAIR 10
Match: AT1G48930.1 (glycosyl hydrolase 9C1 )

HSP 1 Score: 406.0 bits (1042), Expect = 3.6e-113
Identity = 210/467 (44.97%), Postives = 269/467 (57.60%), Query Frame = 0

Query: 24  FDYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFG 83
           ++Y  AL  + L+FE+QRSG LP  QR+ WR  SGL DGL QGV L GGYYDAGD+VKFG
Sbjct: 29  YNYGSALDKTFLFFEAQRSGKLPAAQRVKWRGPSGLKDGLAQGVSLEGGYYDAGDHVKFG 88

Query: 84  LPMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGAT 143
           LPMAF +TMLSW  ++  ++++++ +    L +I+WGTDYFIKAHP PNV W QVGDG +
Sbjct: 89  LPMAFAVTMLSWAAVDNRKELSSSNQMQQTLWSIRWGTDYFIKAHPQPNVLWGQVGDGES 148

Query: 144 DHYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQ 203
           DHYCW+RPEDMTTSR AYK+D  +PGSD+AGETAAA+AAA++ F   N  YS LLL HA+
Sbjct: 149 DHYCWERPEDMTTSRTAYKLDPYHPGSDLAGETAAALAAASLAFKPFNSSYSALLLSHAK 208

Query: 204 E----------------------------------------------------------- 263
           E                                                           
Sbjct: 209 ELFSFADKYRGLYTNSIPNAKAFYMSSGYSDELLWAAAWLHRATGDQYYLKYAMDNSGYM 268

Query: 264 ---------------------------------------------AKHYLCSCLNKNKNN 323
                                                        A ++ C+CL   KN 
Sbjct: 269 GGTGWGVKEFSWDNKYAGVQILLSKILLEGKGGIYTSTLKQYQTKADYFACACL--KKNG 328

Query: 324 STNLKRTPGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLKCNQLEVGPQEMLTLA 383
             N++ TPGGL+Y R+WNNLQY + AA+LL +YSD+L A+N +L C    V PQ +L  A
Sbjct: 329 GYNIQTTPGGLMYVREWNNLQYASAAAYLLAVYSDYLSAANAKLNCPDGLVQPQGLLDFA 388

Query: 384 KSQVDYILGANPMAMSYLVGFGPQYPQRVHHRGASMESIKDNEGFIGCVQGYDNWYGRVD 387
           +SQ DYILG N   MSY+VG+GP+YP RVHHRG+S+ SI      + CVQG+D+WY R  
Sbjct: 389 RSQADYILGKNRQGMSYVVGYGPKYPIRVHHRGSSIPSIFAQRSSVSCVQGFDSWYRRSQ 448

BLAST of Lsi05G005740 vs. TAIR 10
Match: AT2G44550.1 (glycosyl hydrolase 9B10 )

HSP 1 Score: 375.2 bits (962), Expect = 6.9e-104
Identity = 198/458 (43.23%), Postives = 256/458 (55.90%), Query Frame = 0

Query: 25  DYHQALSISLLYFESQRSGHLPYNQRLTWRHHSGLSDGLRQGVDLVGGYYDAGDNVKFGL 84
           +Y +AL  SLLYFE+QRSG LP NQR+TWR  S L DG    +DL GGYYDAGDN+KFG 
Sbjct: 33  NYAEALKNSLLYFEAQRSGKLPPNQRVTWRGDSALRDGSDAHIDLTGGYYDAGDNMKFGF 92

Query: 85  PMAFTITMLSWGVIEYGEQIAAAEEYTHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATD 144
           P+AFT TML+W  IE   Q+ A  E  +AL A+KW TDY IKAHP PNV + QVG+G +D
Sbjct: 93  PLAFTTTMLAWSNIEMASQLRAHHEKGNALRALKWATDYLIKAHPQPNVLYGQVGEGNSD 152

Query: 145 HYCWQRPEDMTTSRRAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHLLLHHAQE 204
           H CW RPEDMTT R +Y+ID  +PGSD+AGETAAAMAAA+I F+ ++  Y+++L+ HA++
Sbjct: 153 HKCWMRPEDMTTPRTSYRIDAQHPGSDLAGETAAAMAAASIAFAPSDKAYANILIGHAKD 212

Query: 205 ------------------------------------------------------------ 264
                                                                       
Sbjct: 213 LFAFAKAHRGLYQNSIPNAGGFYASSGYEDELLWAAAWLHRATNDQIYLDYLTEAETGGP 272

Query: 265 --------------------------------------AKHYLCSCLNKNKNNSTNLKRT 324
                                                 A+ ++C+C  K  N   N+K+T
Sbjct: 273 RTVFAWDDKFVGAQVLVAKLALEGKVESSEQIVEYKSMAEQFICNCAQKGDN---NVKKT 332

Query: 325 PGGLLYTRKWNNLQYVTTAAFLLTLYSDHLQASNQRLKCNQLEVGPQEMLTLAKSQVDYI 384
           PGGLLY   WNNLQY T A F+L+ YS +L+A+   + C    +   ++L +A+SQVDYI
Sbjct: 333 PGGLLYFLPWNNLQYTTAATFVLSAYSKYLEAAKASIDCPDGALQASDLLQVARSQVDYI 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O487661.3e-15558.44Endoglucanase 11 OS=Arabidopsis thaliana OX=3702 GN=At2g32990 PE=2 SV=1[more]
Q6H3Z91.8e-14152.73Endoglucanase 22 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU11 PE=2 SV=2[more]
Q6Z5P21.6e-13450.73Endoglucanase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU10 PE=2 SV=1[more]
Q654U44.4e-13248.22Endoglucanase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0247900 PE=2 S... [more]
Q67UW59.3e-13050.00Endoglucanase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0151300 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1F1P02.3e-18770.04Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111438604 PE=3 SV=1[more]
A0A5A7UIW12.1e-18568.47Cellulase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold104G00290 PE=3... [more]
A0A6J1L8271.2e-18369.42Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111500164 PE=3 SV=1[more]
A0A5D3DKF65.7e-18373.71Cellulase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00470 PE=3... [more]
A0A0A0L5044.7e-17769.15Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G073940 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038904226.11.4e-19972.76endoglucanase 11-like [Benincasa hispida][more]
XP_022932240.14.7e-18770.04endoglucanase 11-like [Cucurbita moschata][more]
KAG7015533.11.0e-18670.10Endoglucanase 11, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAA0055853.14.4e-18568.47endoglucanase 11-like [Cucumis melo var. makuwa][more]
XP_004149358.39.7e-18568.14endoglucanase 11 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G32990.19.1e-15758.44glycosyl hydrolase 9B8 [more]
AT1G64390.12.4e-12546.52glycosyl hydrolase 9C2 [more]
AT4G11050.16.6e-12346.54glycosyl hydrolase 9C3 [more]
AT1G48930.13.6e-11344.97glycosyl hydrolase 9C1 [more]
AT2G44550.16.9e-10443.23glycosyl hydrolase 9B10 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 23..203
e-value: 1.7E-84
score: 286.4
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 204..399
e-value: 3.0E-55
score: 190.2
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 26..205
e-value: 1.6E-71
score: 242.0
NoneNo IPR availablePANTHERPTHR22298:SF137ENDOGLUCANASEcoord: 204..393
coord: 5..204
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 204..393
coord: 5..204
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GH9_2coord: 285..311
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 24..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G005740.1Lsi05G005740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030245 cellulose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008810 cellulase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds