Lsi05G005680 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G005680
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGrpE protein homolog
Locationchr05: 7036514 .. 7041950 (-)
RNA-Seq ExpressionLsi05G005680
SyntenyLsi05G005680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGACTTTAGTCGGTCATAAACATCTATCATTTCTCACTGTTTTCATTTTTAAGTTTTATTAGTCTTAAATTGTATCACTTTTACAGATACCTTTCAATTTGTGCTATATTTGATTTTACTATATTCAATCAACGACTTCTTCCTCAAGCAACTACAACGACGTCGTTTAATAAAACACAAGTGAAGGGGTAGTTTCGTCAATTTCACTTTAAGCCACCGCAAAACCCTTGCTCTTGTTTTCTCTCCTCTGTTCTTCTCCTCCTCAACCACATTCTTCTGTCTCCTCTTCATTTCTTTCCCTTTCTTTGCCTGAAAAAGCTTAATCCGATCTTCCTTTCCAGTTTTTGTTCACCTTCATTGTTAGCTATGTTCGTTACGAAGCTTTTCTCACGCGCCTCCCGCAGTATTTTCCGCAGCTCGCTGCTCTCGTCGGCCTCCCAATCCGATAAAACCATTCACTTGCCAATTCTCACAAATCGCTTACACTATCTTGCTAACGAATCAACCAACAAGGTGGGATTTTAATACCTGCTAGTTTCTCGTTTTTGTTTTCTGCTACGAGGGTTTAGCTAGGGTTTTGGGGGTTTCTCGAAATTTCCAACGTGACAGCAACGTAGTAATTCGAATTCCTTCCATTTTTATGAAGTTTATATGCTTTTAGTCGCATTTGTTACTATTTTCTGTTATGATATGCATTTTGGACCTCCCCTCCATCTTAGTTCTTTTGCTGGGTGTCTTGGATTTGTTATTATTTATGAACTTTGGTGTATTTTTAATATGTGTTCTTTAGTTGGAGAGCGTTTAAATGTCGTGCTGATTGGAACTAGTTTACCCTTGTTCTCATTTCCCCCCCTTTTATTATGAGGAGATCATTGGGATTTATTGCTTAGTTTTTTTGGTTACTTGTATATCTCAAATTATAGGATGTTAAATTATACAACTTTCATGATTCATTGGTGTAATGTGCACAGTTCTATTCCTTTTATGTTCAGGAATCAGCGTTACATCATTCATTTTTCCTTCAAAAATTTGGATTATCAACATCTGCATCCCCTGAGACTTCTAATAAGGAAAATGGCAATACAGTACCAAGTAATGGCGGCTCTACAACCGAGGCAGAGCCGACGGCTGAAACTAATGGTCAAACCCGAGAATTATGTTCCATATCCGGATGTTGTTTTTTCAATGCCTGCCTTTAATGGAGTAGTAACAACTCTGATTTCTGTTTCAGATTCCGAGAGCGATGACGATCTTTCCATGGATGATCTGGTGAAAGTTGTAGCTGAGAAGGAAGAGCTTTTGAAGTTAAAGCACAAAGAGATTGAACAAATGCAAGATAAAGTTGTTCGCACTTATGCAGAGATGGAGAATGTGATGGCCAGGACAAGGCGTGAAGCCGAGAATTCAAAGAAGTTTGCTATACAGGTTTTATTTTATATTTGGCCTATTTCTGGTCTTTGATATTTGTTATTGTGTTGCAATGACATGGGGGAATAAATTAATGTTCACTTGCAAAACGCTCTCCTGACATCAATCATTTATATGTATTTTAGAACTTTGCAAAGAGTTTACTGGACGTTGCAGACAATCTAGGACGAGCTTCATCTGTAGTCAAGGAAAGTTTTTCAAAGATTGATTCAACTAAGGACTCTGCGGGGGCAGTACCCTTATTGAAAACCCTTCTTGAAGGTGTTGAAATGACTGAGAAACAGCTGTCTGAGGTAAAGTATCTCATGTAAAAATGTTATTGAGGTTTGCAGTTAATTTGAATCTATTTTCATTATATTAATAATTGGGTGGTTTAAGTGGTCAAGTTTCTTGAATTTGACGAAAGAAGAACTCATGTCCACTTATTTAGGATGTTGGCATTCCATGTATTTTATAATTCAATTTCTAAGTTGTAGAGTCTGGTGATAGTGTAAAATGGTGAGAATTTTGATTATCAGTTAAACTTATAAGTTATACGAGTAGAATCAGCCAATTGAAACCATCTGCTGTAATACTATAAATTTAAAATCTTAAATGCAACTAAAATAACATGGTAAATAGGTCACTGGGTATCTCCATCTAATTTTGACTCTTCATGGCATTCGTGTGGAAATGAAGTTCAACTAAATTTTGTTGCTTGCAGGTGTTAAAGAAGTTTGGAGTGGAAATATTTGATCCTACGAATGAGCCATTTGATCCACATAGGCACAATGCAGTTTTTCAACTGCCAGATGGTTCTAAGCCTCCTGGTACCGTTGCAGCTGTATTGAAGGTATGTATCATGGTCATGATCATTGTTTTCTGTGTGGTTCCTACCTTTTACCATTATTATATTTTATATGCCTTTATGAAGTTTGTATGATGACGAGTTGTAGGTTAGACAAAGTTTACATTCACAACGATAATTAAAAATAATTAAACGGGATCTTTGTGAACCTTTTCTTTGACCAATTAATTTCTCTTGAGAGCCGTCAGCTTCCAGGTTTTGTTTGTAGCACTAGCACAAGAGAATTGGTTGTAACTTGTAAGTTGTCTAAAGATGTTCGCTTCCTATACATTTAGATGTCGATACAAAATCTTAGGAGATACTCATAGTAAATATGGATGTCTTTTTGGTTTCTACTTGAGAACCTGGTGTAAATTATAAATGTTGATTATTATTATTATTTTAATTTTTGGTGATCATTATGGGCTGATTTTGGGTTAGAAGAAAAAGCTATGAACTCAAATGTTTCAGGGGAAATTTAGCTCACAATTGTGTTAAGCATGGATTTGTATAGCATAAAATTGGGGATTATAAGCTTGTGTAGCCATTGTTTTGGTTGTGTAGGGAGTATAAAATTGGGGAGTACAAGCTTCTATTTGGTTGTATAGAAGAATGAATCAGCTAACCTCGAGTTCATGTAGCTTCTATTTGATTACGTAGCCTGAGAATAGTGTTTTAAAAGTTTAGGTTTAAGTAGCTTGTGGGTTGTCAAATATGTAGCCTTGTATTTTTTAAAGTGGTTAGTGCACTAAAGCACAAAGTGCAAAAATAGGTAAGGTTTCTTTGTTAAACAGCTGAAATGTATAAAGAATTTTTTTTATATAAGAGAAAATATATAGGATTATGAAACATCTATGATCTAAGAAATTAATGTTATAGAGGAGATTGTTCAGAGAAATTAGGGCGTCTGGAGCATTCTTAATTCTTTCATAAGAGGAGGATGGCCTTCATTGGATCATCTTGTTTCCAAGGGGTGAAATTAGATAAGGAAACAACATTGTCATTTAATCTATTGCTCTCAAATTTGTTCTTGAGCTTGTGGCCCCTACACTAGGAGTGCTGCTCAAGCGAACACGATTGGGTCTTTCATCAAATGAGAATAATTTCTTAAAGAAATAGGTTAAAAATTCTTAGTGATCCTCAGCTACGATGAACAAATCCCCCATTGTACCACATTTGTTTAAATCCCAAGCATTATAGCAAGAATAAATCGTGAATGATATTAAAATAGGAGATCAACATATGAACTACACATCCTTAATCTAATCAACATCACTAGCATTGGAAAAAAACGCTTCAAAACCCAAAAATGAATAACAATTAGAAAAGAGAATTAAAACCTTGATGTCTCATGAGATGCGTTGGAGTGGGGGGGGGGGGGGGGGTTGGGGGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGATTTGGGGGCCTGATACCATGGGGGCTATAAATGCTTGGGAAAAGTTTCTCGAGATACAAGGTTTGAGCCTCTAAATGGATTCCTCCTGGGCCCAAATAACTTAATTTTCTCCCATGAACAATTTGTGTCAATAAATGAAAAGCTTCTTCTAGAGTTGGCATTGAGGTGTGCGATGCTATCTATAAATTAAACCAGATGTAATGTAATCAATATTTTAAAATATCAAGTTAAAAGCAAAACTTCAATCGAATACATCTATAACTGGGTTGATTATATATACACATGTATGTGTATGTGTGTTCTGATATTACATTAGAGTTATCTAGTTGTCAAAGACTCTTTGGGTAACTGTATCTCCGAATTCCACAAGTTTTAACAGAATTGAAATAGGATATCCATTAGCCTTTCTGTCAGGTTTTTGGCTATGTTTCATATGCAAAGTTTGTTTTTCTAAACAAACGTAAATGCTAAGCCCGGTAGCATGGGCTTGTTTCCCTAGCGGGGGCAAATTCAAACACAATAACTAAACATGAAAACAGTATACTTGCATCTGTATCTATCTTGTTGAAGCAGAACTAATGTTACAACTTGCATCAAAGCTATGATAATTGGAAAAGCCTAACTGTTATTTTGTTGTTTATGTCAATAGAAAGGATACATGCTTTACGAGCGAGTACTTCGACCTGCCGAAGTTGGTGTTACGAAAGCTGTGGAGAATGAGGACGGTGCAACCGATGATGCAAATGAGAAGGGATCTCAAGGTTGAAGGGTTGGAAGGATGGATAGTTTCCTCTAAAAAAAGAAGTTTTGCTATACGGAATGTTCTTTGGTAGAGAAGATACCTAATCTAGACTACTTGTTAGATATGCACAGGGAAAGAAAAACAATTCATTGTTGGCATAAAATTTGATTTTGTTATCTATTTAGAGAGAGAGATGAGAGTATTTCCATGATTTCCTGTCAGCTCAAATTCAGAATGTGTATTTTGATACCATAAGCCTTCATGTGTAAACATTTTCGTTGAGGCATTTTTCTTGTTGAAAAGTTAACAAAGGGCTTTCTGTCAAGGATGAACAACTTGAAAAATGGTCTTGCTTATGTCAGCTGCGTGTGAAAGTACCATCTTGTCTTTAAATCACGTGT

mRNA sequence

ATAGACTTTAGTCGGTCATAAACATCTATCATTTCTCACTGTTTTCATTTTTAAGTTTTATTAGTCTTAAATTGTATCACTTTTACAGATACCTTTCAATTTGTGCTATATTTGATTTTACTATATTCAATCAACGACTTCTTCCTCAAGCAACTACAACGACGTCGTTTAATAAAACACAAGTGAAGGGGTAGTTTCGTCAATTTCACTTTAAGCCACCGCAAAACCCTTGCTCTTGTTTTCTCTCCTCTGTTCTTCTCCTCCTCAACCACATTCTTCTGTCTCCTCTTCATTTCTTTCCCTTTCTTTGCCTGAAAAAGCTTAATCCGATCTTCCTTTCCAGTTTTTGTTCACCTTCATTGTTAGCTATGTTCGTTACGAAGCTTTTCTCACGCGCCTCCCGCAGTATTTTCCGCAGCTCGCTGCTCTCGTCGGCCTCCCAATCCGATAAAACCATTCACTTGCCAATTCTCACAAATCGCTTACACTATCTTGCTAACGAATCAACCAACAAGGAATCAGCGTTACATCATTCATTTTTCCTTCAAAAATTTGGATTATCAACATCTGCATCCCCTGAGACTTCTAATAAGGAAAATGGCAATACAGTACCAAGTAATGGCGGCTCTACAACCGAGGCAGAGCCGACGGCTGAAACTAATGATTCCGAGAGCGATGACGATCTTTCCATGGATGATCTGGTGAAAGTTGTAGCTGAGAAGGAAGAGCTTTTGAAGTTAAAGCACAAAGAGATTGAACAAATGCAAGATAAAGTTGTTCGCACTTATGCAGAGATGGAGAATGTGATGGCCAGGACAAGGCGTGAAGCCGAGAATTCAAAGAAGTTTGCTATACAGAACTTTGCAAAGAGTTTACTGGACGTTGCAGACAATCTAGGACGAGCTTCATCTGTAGTCAAGGAAAGTTTTTCAAAGATTGATTCAACTAAGGACTCTGCGGGGGCAGTACCCTTATTGAAAACCCTTCTTGAAGGTGTTGAAATGACTGAGAAACAGCTGTCTGAGGTGTTAAAGAAGTTTGGAGTGGAAATATTTGATCCTACGAATGAGCCATTTGATCCACATAGGCACAATGCAGTTTTTCAACTGCCAGATGGTTCTAAGCCTCCTGGTACCGTTGCAGCTGTATTGAAGAAAGGATACATGCTTTACGAGCGAGTACTTCGACCTGCCGAAGTTGGTGTTACGAAAGCTGTGGAGAATGAGGACGGTGCAACCGATGATGCAAATGAGAAGGGATCTCAAGGTTGAAGGGTTGGAAGGATGGATAGTTTCCTCTAAAAAAAGAAGTTTTGCTATACGGAATGTTCTTTGGTAGAGAAGATACCTAATCTAGACTACTTGTTAGATATGCACAGGGAAAGAAAAACAATTCATTGTTGGCATAAAATTTGATTTTGTTATCTATTTAGAGAGAGAGATGAGAGTATTTCCATGATTTCCTGTCAGCTCAAATTCAGAATGTGTATTTTGATACCATAAGCCTTCATGTGTAAACATTTTCGTTGAGGCATTTTTCTTGTTGAAAAGTTAACAAAGGGCTTTCTGTCAAGGATGAACAACTTGAAAAATGGTCTTGCTTATGTCAGCTGCGTGTGAAAGTACCATCTTGTCTTTAAATCACGTGT

Coding sequence (CDS)

ATGTTCGTTACGAAGCTTTTCTCACGCGCCTCCCGCAGTATTTTCCGCAGCTCGCTGCTCTCGTCGGCCTCCCAATCCGATAAAACCATTCACTTGCCAATTCTCACAAATCGCTTACACTATCTTGCTAACGAATCAACCAACAAGGAATCAGCGTTACATCATTCATTTTTCCTTCAAAAATTTGGATTATCAACATCTGCATCCCCTGAGACTTCTAATAAGGAAAATGGCAATACAGTACCAAGTAATGGCGGCTCTACAACCGAGGCAGAGCCGACGGCTGAAACTAATGATTCCGAGAGCGATGACGATCTTTCCATGGATGATCTGGTGAAAGTTGTAGCTGAGAAGGAAGAGCTTTTGAAGTTAAAGCACAAAGAGATTGAACAAATGCAAGATAAAGTTGTTCGCACTTATGCAGAGATGGAGAATGTGATGGCCAGGACAAGGCGTGAAGCCGAGAATTCAAAGAAGTTTGCTATACAGAACTTTGCAAAGAGTTTACTGGACGTTGCAGACAATCTAGGACGAGCTTCATCTGTAGTCAAGGAAAGTTTTTCAAAGATTGATTCAACTAAGGACTCTGCGGGGGCAGTACCCTTATTGAAAACCCTTCTTGAAGGTGTTGAAATGACTGAGAAACAGCTGTCTGAGGTGTTAAAGAAGTTTGGAGTGGAAATATTTGATCCTACGAATGAGCCATTTGATCCACATAGGCACAATGCAGTTTTTCAACTGCCAGATGGTTCTAAGCCTCCTGGTACCGTTGCAGCTGTATTGAAGAAAGGATACATGCTTTACGAGCGAGTACTTCGACCTGCCGAAGTTGGTGTTACGAAAGCTGTGGAGAATGAGGACGGTGCAACCGATGATGCAAATGAGAAGGGATCTCAAGGTTGA

Protein sequence

MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQKFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETNDSESDDDLSMDDLVKVVAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDANEKGSQG
Homology
BLAST of Lsi05G005680 vs. ExPASy Swiss-Prot
Match: Q8LB47 (GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-81
Identity = 181/325 (55.69%), Postives = 229/325 (70.46%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNK----ESALHHS 60
           M V ++ SR +R+    S LS+ +   +    P+ ++R H LA++ ++K    + ++  S
Sbjct: 1   MLVLRILSRVTRNAGIRSSLSAVTLPARN-QTPVFSSRFHSLAHDFSHKLVPAQMSMMDS 60

Query: 61  FFLQKFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------------------ 120
           F LQ+F  S+S SPE+  K+      +    T+E +PTAE N                  
Sbjct: 61  FALQRFNFSSSTSPESDEKK----THTEASKTSEEKPTAEANQPGLDSESKDSVTDSAKR 120

Query: 121 ---------------DSESDDD-LSMDDLVKVVAEKEELLKLKHKEIEQMQDKVVRTYAE 180
                          DSESDDD LS DDLVK+VAEKEELL  K +EI+Q++DKV+RTYAE
Sbjct: 121 KRKGAKGAASSSSESDSESDDDELSADDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAE 180

Query: 181 MENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKESFSKIDSTKDSAGAVPL 240
           MENVM RTRR+AEN+KK+A+QNFAKSLLDVADNLGRASSVVKESFSK+D+++DSAGA PL
Sbjct: 181 MENVMDRTRRDAENTKKYAVQNFAKSLLDVADNLGRASSVVKESFSKLDTSEDSAGAAPL 240

Query: 241 LKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLK 288
           LKTLLEGVEMTEKQL+EV KKFG+E +DP NEPFDP+RHNAVFQ+PD SKP GTVA VLK
Sbjct: 241 LKTLLEGVEMTEKQLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLK 300

BLAST of Lsi05G005680 vs. ExPASy Swiss-Prot
Match: Q9FLP3 (GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE=2 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.1e-75
Identity = 170/303 (56.11%), Postives = 215/303 (70.96%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSF--- 60
           M V+++ SR SRS    S  SS   + K   +PI+ +R H L + + NK  A+  S    
Sbjct: 1   MLVSRVLSRVSRSAGLRSSFSSV-VTPKRNQIPIVASRFHSLVHGTPNKLVAVPVSLRNH 60

Query: 61  ------FLQKFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETNDSESD-DDLSMDD 120
                  LQ+FG  +S+S E    E+   VP   G T+E     +  D+E D DDLS DD
Sbjct: 61  GTLDLNVLQRFGFFSSSSAEPKGNESNTEVPKT-GETSENVEVGKATDAEIDFDDLSRDD 120

Query: 121 LVKVVAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLL 180
           LVK+V+EKE+LLK++ K+I +M+DK +RTYAE +N+M RT R AE++KKFA+QNFA SLL
Sbjct: 121 LVKLVSEKEDLLKVQQKDIMEMKDKFLRTYAEQQNLMDRTNRNAESAKKFAVQNFATSLL 180

Query: 181 DVADNLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFD 240
           DVADNL RASSVVKESFSKID++KD AGA PLLK LLEGVEMTEKQL+EV +K G+   D
Sbjct: 181 DVADNLERASSVVKESFSKIDTSKDLAGATPLLKNLLEGVEMTEKQLAEVFRKAGLVKED 240

Query: 241 PTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGAT 294
           P NEPF+P+RHNAVFQ+PD SKP GT+A VLK GY LY+RV+RPAEVGVT AVEN++G  
Sbjct: 241 PLNEPFNPNRHNAVFQVPDASKPKGTIAHVLKSGYSLYDRVIRPAEVGVTCAVENQEGGK 300

BLAST of Lsi05G005680 vs. ExPASy Swiss-Prot
Match: Q3SW78 (Protein GrpE OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=grpE PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.4e-29
Identity = 82/199 (41.21%), Postives = 117/199 (58.79%), Query Frame = 0

Query: 83  SNGGSTTEAEPTAETNDSESDDDLSMDDLVKVVAEKEELLKLKHKEIEQMQDKVVRTYAE 142
           S+G +    EP AE     S   +  DD       +++ L   +K++ + +D+ +RT AE
Sbjct: 4   SDGKTDKSGEPAAEVEPVVSKPYVMPDD------PEDDALDALNKQLAEAKDRTLRTLAE 63

Query: 143 MENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKESFSKIDSTKDSAGAVPL 202
           MEN+  RT RE  +++ + I  FA+ +L++ADNL RA   V             A   P 
Sbjct: 64  MENLRKRTAREVSDARTYGISGFARDVLEIADNLQRALDAV--------PADARAAPDPG 123

Query: 203 LKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLK 262
           LK L+EGVE+TE+ L   L+K GV+ FDP  E FDP+ H A++++PD S P GTVA V++
Sbjct: 124 LKALIEGVELTERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIPVGTVAQVIQ 183

Query: 263 KGYMLYERVLRPAEVGVTK 282
            GYM+ ERVLRPA VGV K
Sbjct: 184 AGYMIGERVLRPALVGVAK 188

BLAST of Lsi05G005680 vs. ExPASy Swiss-Prot
Match: A4YJR1 (Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.0e-29
Identity = 72/155 (46.45%), Postives = 102/155 (65.81%), Query Frame = 0

Query: 127 KEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKES 186
           KE  + +DK++RT AEMEN+  RT RE  +++ + I  FA+ +LD+ADNL RA   V   
Sbjct: 42  KEAAEARDKMLRTLAEMENLRKRTAREVADARMYGITGFARDVLDIADNLQRALDAV--- 101

Query: 187 FSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRHNAVFQ 246
                  +  A A   LK+L+EGVE+TE+ L   L+K GV+ FDPT + FDP+   A+++
Sbjct: 102 -----PAETRANADAGLKSLIEGVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYE 161

Query: 247 LPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTK 282
           +PD S P GTV  V++ G+M+ ERVLRPA VGV+K
Sbjct: 162 VPDPSVPSGTVVQVVQAGFMIGERVLRPALVGVSK 188

BLAST of Lsi05G005680 vs. ExPASy Swiss-Prot
Match: Q79V15 (Protein GrpE OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=grpE PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-28
Identity = 71/160 (44.38%), Postives = 102/160 (63.75%), Query Frame = 0

Query: 122 LKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASS 181
           ++L  KE  + +D+++RT AEMEN+  RT +E  +++ + I  FA+ +LD+ADNL RA  
Sbjct: 35  VELLQKEAAEARDRMLRTLAEMENLRKRTTKEVADARLYGITGFARDVLDIADNLQRALD 94

Query: 182 VVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRH 241
            V          +  A A P L +L+EGVE+TE+ L   L+K GV+ FDP  + FDP+  
Sbjct: 95  AV--------PAEARAAADPGLTSLIEGVELTERSLLNALEKHGVKKFDPQGQKFDPNFQ 154

Query: 242 NAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTK 282
            A+F++PD S P GTV  V++ GY + ERVLRPA VGV K
Sbjct: 155 QAMFEVPDASVPAGTVVQVMQAGYTIGERVLRPALVGVAK 186

BLAST of Lsi05G005680 vs. ExPASy TrEMBL
Match: A0A1S3BLD9 (GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103491087 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.3e-139
Identity = 268/300 (89.33%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA+QSDK IHLPIL NR H LAN+S +KES LH SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAAQSDKPIHLPILANRFHSLANQSNHKESTLHRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETNDSESDDDLSMDDLVKVVAEKEE 120
           KFGLSTSASPETSNKENGN+ PSNG S T+AEPTAETNDSESDD+LS+DDLVKVVAEKEE
Sbjct: 61  KFGLSTSASPETSNKENGNSAPSNGSSKTDAEPTAETNDSESDDNLSIDDLVKVVAEKEE 120

Query: 121 LLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRAS 180
           LLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFA+QNFAKSLLDVADNLGRAS
Sbjct: 121 LLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAVQNFAKSLLDVADNLGRAS 180

Query: 181 SVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHR 240
           SVVKESFSKIDST DSAGAVPLLKTLLEGVEMTEKQLSEVLKK+GVE+FDPTNEPFDPHR
Sbjct: 181 SVVKESFSKIDSTNDSAGAVPLLKTLLEGVEMTEKQLSEVLKKYGVEMFDPTNEPFDPHR 240

Query: 241 HNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDANEKGSQG 300
           HNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA EKGSQG
Sbjct: 241 HNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDATEKGSQG 300

BLAST of Lsi05G005680 vs. ExPASy TrEMBL
Match: A0A5A7UL93 (GrpE protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00420 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 7.8e-137
Identity = 268/306 (87.58%), Postives = 285/306 (93.14%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA+QSDK IHLPIL NR H LAN+S +KES LH SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAAQSDKPIHLPILANRFHSLANQSNHKESTLHRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------DSESDDDLSMDDLVKV 120
           KFGLSTSASPETSNKENGN+ PSNG S T+AEPTAETN      DSESDD+LS+DDLVKV
Sbjct: 61  KFGLSTSASPETSNKENGNSAPSNGSSKTDAEPTAETNGRTRESDSESDDNLSIDDLVKV 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           VAEKEELLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFA+QNFAKSLLDVAD
Sbjct: 121 VAEKEELLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAVQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVKESFSKIDST DSAGAVPLLKTLLEGVEMTEKQLSEVLKK+GVE+FDPTNE
Sbjct: 181 NLGRASSVVKESFSKIDSTNDSAGAVPLLKTLLEGVEMTEKQLSEVLKKYGVEMFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDAN 300
           PFDPHRHNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA 
Sbjct: 241 PFDPHRHNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDAT 300

BLAST of Lsi05G005680 vs. ExPASy TrEMBL
Match: A0A1S3BKR7 (GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103491087 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 7.8e-137
Identity = 268/306 (87.58%), Postives = 285/306 (93.14%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA+QSDK IHLPIL NR H LAN+S +KES LH SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAAQSDKPIHLPILANRFHSLANQSNHKESTLHRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------DSESDDDLSMDDLVKV 120
           KFGLSTSASPETSNKENGN+ PSNG S T+AEPTAETN      DSESDD+LS+DDLVKV
Sbjct: 61  KFGLSTSASPETSNKENGNSAPSNGSSKTDAEPTAETNGRTRESDSESDDNLSIDDLVKV 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           VAEKEELLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFA+QNFAKSLLDVAD
Sbjct: 121 VAEKEELLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAVQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVKESFSKIDST DSAGAVPLLKTLLEGVEMTEKQLSEVLKK+GVE+FDPTNE
Sbjct: 181 NLGRASSVVKESFSKIDSTNDSAGAVPLLKTLLEGVEMTEKQLSEVLKKYGVEMFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDAN 300
           PFDPHRHNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA 
Sbjct: 241 PFDPHRHNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDAT 300

BLAST of Lsi05G005680 vs. ExPASy TrEMBL
Match: A0A0A0L683 (GrpE protein homolog OS=Cucumis sativus OX=3659 GN=Csa_3G073880 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.3e-135
Identity = 266/306 (86.93%), Postives = 283/306 (92.48%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA QSDK IH PILTNRLH LAN+S +KES L+ SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAPQSDKPIHFPILTNRLHSLANQSKDKESTLYRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------DSESDDDLSMDDLVKV 120
           K GLSTSASPETSNKENGNTVP NGGS T AEPTAETN      DS+SDD+LSMDDLVKV
Sbjct: 61  KHGLSTSASPETSNKENGNTVPRNGGSKTNAEPTAETNGRTGESDSDSDDNLSMDDLVKV 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           VAEKEELLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFAIQNFAKSLLDVAD
Sbjct: 121 VAEKEELLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAIQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVK SFSKIDST DS+GAVPLLKTL++GVEMTEKQLSEVLKK+GVE+FDPTNE
Sbjct: 181 NLGRASSVVKGSFSKIDSTNDSSGAVPLLKTLIDGVEMTEKQLSEVLKKYGVEMFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDAN 300
           PFDPHRHNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA 
Sbjct: 241 PFDPHRHNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDAT 300

BLAST of Lsi05G005680 vs. ExPASy TrEMBL
Match: A0A6J1DCM0 (GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111019268 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.2e-129
Identity = 257/293 (87.71%), Postives = 270/293 (92.15%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTK+FS A R+I RSSLLSSASQSDKT HLPIL N LHY  N+  +KESALHHSFFLQ
Sbjct: 1   MFVTKIFSGACRNICRSSLLSSASQSDKTTHLPILANHLHYRVNQPIDKESALHHSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAET------NDSESDDDLSMDDLVKV 120
           KF LSTSASPETS+KENGNTVPSNGGST  +EPTAET      +DSESDD+LSMDDLVK+
Sbjct: 61  KFRLSTSASPETSDKENGNTVPSNGGSTPASEPTAETTGQPKESDSESDDNLSMDDLVKL 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           V EKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD
Sbjct: 121 VTEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVKESFSKIDST DS+GAVPLLKTLLEGVEMTEKQLSEVLKKFGVE FDPTNE
Sbjct: 181 NLGRASSVVKESFSKIDSTDDSSGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEKFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENED 288
           PFDPH HNAVFQ+PDGSKPPGTVAAVLKKGYMLY+RVLRPAEVGVT+AVENED
Sbjct: 241 PFDPHMHNAVFQVPDGSKPPGTVAAVLKKGYMLYDRVLRPAEVGVTRAVENED 293

BLAST of Lsi05G005680 vs. NCBI nr
Match: XP_038903423.1 (grpE protein homolog 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 524.2 bits (1349), Expect = 7.2e-145
Identity = 284/306 (92.81%), Postives = 293/306 (95.75%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNK+SALHHSFFLQ
Sbjct: 1   MFVTKLFSRSSRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKKSALHHSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------DSESDDDLSMDDLVKV 120
           KFGLSTS SPETSNKENGNTVPSNGGSTT+AEP AETN      D ESDD+L+MDDLVKV
Sbjct: 61  KFGLSTSPSPETSNKENGNTVPSNGGSTTDAEPKAETNGQTQESDIESDDNLTMDDLVKV 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           VAEKEELLKLKH+EIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD
Sbjct: 121 VAEKEELLKLKHREIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE
Sbjct: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDAN 300
           PFDPHRHNAVFQ+PDGSKPPGTVA VLKKGYMLYERVLRPAEVGVT A+ENED ATDDA 
Sbjct: 241 PFDPHRHNAVFQVPDGSKPPGTVAIVLKKGYMLYERVLRPAEVGVTTAMENEDDATDDAT 300

BLAST of Lsi05G005680 vs. NCBI nr
Match: XP_008449137.1 (PREDICTED: protein GrpE-like isoform X2 [Cucumis melo])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 268/300 (89.33%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA+QSDK IHLPIL NR H LAN+S +KES LH SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAAQSDKPIHLPILANRFHSLANQSNHKESTLHRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETNDSESDDDLSMDDLVKVVAEKEE 120
           KFGLSTSASPETSNKENGN+ PSNG S T+AEPTAETNDSESDD+LS+DDLVKVVAEKEE
Sbjct: 61  KFGLSTSASPETSNKENGNSAPSNGSSKTDAEPTAETNDSESDDNLSIDDLVKVVAEKEE 120

Query: 121 LLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRAS 180
           LLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFA+QNFAKSLLDVADNLGRAS
Sbjct: 121 LLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAVQNFAKSLLDVADNLGRAS 180

Query: 181 SVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHR 240
           SVVKESFSKIDST DSAGAVPLLKTLLEGVEMTEKQLSEVLKK+GVE+FDPTNEPFDPHR
Sbjct: 181 SVVKESFSKIDSTNDSAGAVPLLKTLLEGVEMTEKQLSEVLKKYGVEMFDPTNEPFDPHR 240

Query: 241 HNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDANEKGSQG 300
           HNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA EKGSQG
Sbjct: 241 HNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDATEKGSQG 300

BLAST of Lsi05G005680 vs. NCBI nr
Match: XP_008449136.1 (PREDICTED: protein GrpE-like isoform X1 [Cucumis melo] >KAA0055848.1 protein GrpE-like isoform X1 [Cucumis melo var. makuwa] >TYK24072.1 protein GrpE-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 496.5 bits (1277), Expect = 1.6e-136
Identity = 268/306 (87.58%), Postives = 285/306 (93.14%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA+QSDK IHLPIL NR H LAN+S +KES LH SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAAQSDKPIHLPILANRFHSLANQSNHKESTLHRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------DSESDDDLSMDDLVKV 120
           KFGLSTSASPETSNKENGN+ PSNG S T+AEPTAETN      DSESDD+LS+DDLVKV
Sbjct: 61  KFGLSTSASPETSNKENGNSAPSNGSSKTDAEPTAETNGRTRESDSESDDNLSIDDLVKV 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           VAEKEELLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFA+QNFAKSLLDVAD
Sbjct: 121 VAEKEELLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAVQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVKESFSKIDST DSAGAVPLLKTLLEGVEMTEKQLSEVLKK+GVE+FDPTNE
Sbjct: 181 NLGRASSVVKESFSKIDSTNDSAGAVPLLKTLLEGVEMTEKQLSEVLKKYGVEMFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDAN 300
           PFDPHRHNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA 
Sbjct: 241 PFDPHRHNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDAT 300

BLAST of Lsi05G005680 vs. NCBI nr
Match: XP_004149344.1 (grpE protein homolog 2, mitochondrial [Cucumis sativus] >KGN56097.1 hypothetical protein Csa_010236 [Cucumis sativus])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 266/306 (86.93%), Postives = 283/306 (92.48%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTKLFSR+SR+I RSSLLSSA QSDK IH PILTNRLH LAN+S +KES L+ SFFLQ
Sbjct: 1   MFVTKLFSRSSRTILRSSLLSSAPQSDKPIHFPILTNRLHSLANQSKDKESTLYRSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------DSESDDDLSMDDLVKV 120
           K GLSTSASPETSNKENGNTVP NGGS T AEPTAETN      DS+SDD+LSMDDLVKV
Sbjct: 61  KHGLSTSASPETSNKENGNTVPRNGGSKTNAEPTAETNGRTGESDSDSDDNLSMDDLVKV 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           VAEKEELLK+KHKEIEQMQDKV+RTYAEMENVMART+REAENSKKFAIQNFAKSLLDVAD
Sbjct: 121 VAEKEELLKVKHKEIEQMQDKVIRTYAEMENVMARTKREAENSKKFAIQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVK SFSKIDST DS+GAVPLLKTL++GVEMTEKQLSEVLKK+GVE+FDPTNE
Sbjct: 181 NLGRASSVVKGSFSKIDSTNDSSGAVPLLKTLIDGVEMTEKQLSEVLKKYGVEMFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGATDDAN 300
           PFDPHRHNAVFQ+PDGSK PGTVAAVLKKGYML+ERVLRPAEVGVTKAVENEDGAT DA 
Sbjct: 241 PFDPHRHNAVFQVPDGSKAPGTVAAVLKKGYMLHERVLRPAEVGVTKAVENEDGATGDAT 300

BLAST of Lsi05G005680 vs. NCBI nr
Match: XP_022151304.1 (uncharacterized protein LOC111019268 [Momordica charantia])

HSP 1 Score: 472.6 bits (1215), Expect = 2.5e-129
Identity = 257/293 (87.71%), Postives = 270/293 (92.15%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQ 60
           MFVTK+FS A R+I RSSLLSSASQSDKT HLPIL N LHY  N+  +KESALHHSFFLQ
Sbjct: 1   MFVTKIFSGACRNICRSSLLSSASQSDKTTHLPILANHLHYRVNQPIDKESALHHSFFLQ 60

Query: 61  KFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAET------NDSESDDDLSMDDLVKV 120
           KF LSTSASPETS+KENGNTVPSNGGST  +EPTAET      +DSESDD+LSMDDLVK+
Sbjct: 61  KFRLSTSASPETSDKENGNTVPSNGGSTPASEPTAETTGQPKESDSESDDNLSMDDLVKL 120

Query: 121 VAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180
           V EKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD
Sbjct: 121 VTEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVAD 180

Query: 181 NLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNE 240
           NLGRASSVVKESFSKIDST DS+GAVPLLKTLLEGVEMTEKQLSEVLKKFGVE FDPTNE
Sbjct: 181 NLGRASSVVKESFSKIDSTDDSSGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEKFDPTNE 240

Query: 241 PFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENED 288
           PFDPH HNAVFQ+PDGSKPPGTVAAVLKKGYMLY+RVLRPAEVGVT+AVENED
Sbjct: 241 PFDPHMHNAVFQVPDGSKPPGTVAAVLKKGYMLYDRVLRPAEVGVTRAVENED 293

BLAST of Lsi05G005680 vs. TAIR 10
Match: AT4G26780.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 304.7 bits (779), Expect = 8.3e-83
Identity = 181/325 (55.69%), Postives = 229/325 (70.46%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNK----ESALHHS 60
           M V ++ SR +R+    S LS+ +   +    P+ ++R H LA++ ++K    + ++  S
Sbjct: 1   MLVLRILSRVTRNAGIRSSLSAVTLPARN-QTPVFSSRFHSLAHDFSHKLVPAQMSMMDS 60

Query: 61  FFLQKFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETN------------------ 120
           F LQ+F  S+S SPE+  K+      +    T+E +PTAE N                  
Sbjct: 61  FALQRFNFSSSTSPESDEKK----THTEASKTSEEKPTAEANQPGLDSESKDSVTDSAKR 120

Query: 121 ---------------DSESDDD-LSMDDLVKVVAEKEELLKLKHKEIEQMQDKVVRTYAE 180
                          DSESDDD LS DDLVK+VAEKEELL  K +EI+Q++DKV+RTYAE
Sbjct: 121 KRKGAKGAASSSSESDSESDDDELSADDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAE 180

Query: 181 MENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKESFSKIDSTKDSAGAVPL 240
           MENVM RTRR+AEN+KK+A+QNFAKSLLDVADNLGRASSVVKESFSK+D+++DSAGA PL
Sbjct: 181 MENVMDRTRRDAENTKKYAVQNFAKSLLDVADNLGRASSVVKESFSKLDTSEDSAGAAPL 240

Query: 241 LKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLK 288
           LKTLLEGVEMTEKQL+EV KKFG+E +DP NEPFDP+RHNAVFQ+PD SKP GTVA VLK
Sbjct: 241 LKTLLEGVEMTEKQLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLK 300

BLAST of Lsi05G005680 vs. TAIR 10
Match: AT5G55200.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.5e-76
Identity = 170/303 (56.11%), Postives = 215/303 (70.96%), Query Frame = 0

Query: 1   MFVTKLFSRASRSIFRSSLLSSASQSDKTIHLPILTNRLHYLANESTNKESALHHSF--- 60
           M V+++ SR SRS    S  SS   + K   +PI+ +R H L + + NK  A+  S    
Sbjct: 1   MLVSRVLSRVSRSAGLRSSFSSV-VTPKRNQIPIVASRFHSLVHGTPNKLVAVPVSLRNH 60

Query: 61  ------FLQKFGLSTSASPETSNKENGNTVPSNGGSTTEAEPTAETNDSESD-DDLSMDD 120
                  LQ+FG  +S+S E    E+   VP   G T+E     +  D+E D DDLS DD
Sbjct: 61  GTLDLNVLQRFGFFSSSSAEPKGNESNTEVPKT-GETSENVEVGKATDAEIDFDDLSRDD 120

Query: 121 LVKVVAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFAKSLL 180
           LVK+V+EKE+LLK++ K+I +M+DK +RTYAE +N+M RT R AE++KKFA+QNFA SLL
Sbjct: 121 LVKLVSEKEDLLKVQQKDIMEMKDKFLRTYAEQQNLMDRTNRNAESAKKFAVQNFATSLL 180

Query: 181 DVADNLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFD 240
           DVADNL RASSVVKESFSKID++KD AGA PLLK LLEGVEMTEKQL+EV +K G+   D
Sbjct: 181 DVADNLERASSVVKESFSKIDTSKDLAGATPLLKNLLEGVEMTEKQLAEVFRKAGLVKED 240

Query: 241 PTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENEDGAT 294
           P NEPF+P+RHNAVFQ+PD SKP GT+A VLK GY LY+RV+RPAEVGVT AVEN++G  
Sbjct: 241 PLNEPFNPNRHNAVFQVPDASKPKGTIAHVLKSGYSLYDRVIRPAEVGVTCAVENQEGGK 300

BLAST of Lsi05G005680 vs. TAIR 10
Match: AT5G17710.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-08
Identity = 53/194 (27.32%), Postives = 85/194 (43.81%), Query Frame = 0

Query: 107 SMDDLVKVVAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFA 166
           S++D   ++A+K   L     E+   +D+++R  A+ +N   RT RE  N    A     
Sbjct: 138 SIEDEKFLLADKVASLS---NELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEVV 197

Query: 167 KSLLDVADNLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGV 226
           ++LL V DN  RA S +K      +   +S  ++              KQ  E+L   GV
Sbjct: 198 ENLLAVLDNFERAKSQIKVETEGEEKVTNSYQSI-------------YKQFVEILGSLGV 257

Query: 227 EIFDPTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENE 286
              +   + FDP  H A+ +        G V    +KG++L ER+LRP+ V V+     E
Sbjct: 258 IHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPE 315

Query: 287 DGATDDANEKGSQG 301
                +  E  +QG
Sbjct: 318 KPLEAEGEEATAQG 315

BLAST of Lsi05G005680 vs. TAIR 10
Match: AT5G17710.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-08
Identity = 53/194 (27.32%), Postives = 85/194 (43.81%), Query Frame = 0

Query: 107 SMDDLVKVVAEKEELLKLKHKEIEQMQDKVVRTYAEMENVMARTRREAENSKKFAIQNFA 166
           S++D   ++A+K   L     E+   +D+++R  A+ +N   RT RE  N    A     
Sbjct: 140 SIEDEKFLLADKVASLS---NELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEVV 199

Query: 167 KSLLDVADNLGRASSVVKESFSKIDSTKDSAGAVPLLKTLLEGVEMTEKQLSEVLKKFGV 226
           ++LL V DN  RA S +K      +   +S  ++              KQ  E+L   GV
Sbjct: 200 ENLLAVLDNFERAKSQIKVETEGEEKVTNSYQSI-------------YKQFVEILGSLGV 259

Query: 227 EIFDPTNEPFDPHRHNAVFQLPDGSKPPGTVAAVLKKGYMLYERVLRPAEVGVTKAVENE 286
              +   + FDP  H A+ +        G V    +KG++L ER+LRP+ V V+     E
Sbjct: 260 IHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPE 317

Query: 287 DGATDDANEKGSQG 301
                +  E  +QG
Sbjct: 320 KPLEAEGEEATAQG 317

BLAST of Lsi05G005680 vs. TAIR 10
Match: AT1G36390.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 45.4 bits (106), Expect = 9.1e-05
Identity = 71/271 (26.20%), Postives = 120/271 (44.28%), Query Frame = 0

Query: 21  SSASQSDKTIHLPILTNRLHYLANESTNKESALHHSFFLQKFGLSTSASPETSNKENGNT 80
           SS S S KT  LP L N LH+    S         S   +KF +   ++ +T+N E  N+
Sbjct: 18  SSPSSSSKT--LP-LRNNLHFHGRPSPISPLISKPS---RKFPI--FSAHQTNNSEEANS 77

Query: 81  VPSNGGST-TEAEPTAETNDSESDDDLSMDDLVKVVAEK----EELLKLKHKEIEQMQDK 140
                  T   +   A  N  E+          K+  EK    +++L L  K I   ++ 
Sbjct: 78  KQQADVKTLIRSYKQALLNGDETSVTEIETMFCKIEKEKNKMDQKVLSLSMK-IASEKEM 137

Query: 141 VVRTYAEMENVMARTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKESFSKIDSTKD 200
            +R  A+ +N   +  ++  +++  A     KSLL + D+  +A   V     ++D+ K+
Sbjct: 138 KIRLQADFDNTRKKLDKDRLSTESNAKVQILKSLLPIIDSFEKAKLQV-----RVDTDKE 197

Query: 201 SAGAVPLLKTLLEGVEMTEKQLSEVLKKFGVEIFDPTNEPFDPHRHNAVFQLPDGSKPPG 260
                  + T  +G+    +Q  EVL+   V +     +PFDP  H A+ +    +   G
Sbjct: 198 KK-----IDTSYQGI---YRQFVEVLRYLRVSVIATVGKPFDPLLHEAISREESEAVKAG 257

Query: 261 TVAAVLKKGYMLYERVLRPAEVGVTKAVENE 287
            +   L KG++L +RVLRPA+V V+    N+
Sbjct: 258 IITEELNKGFVLGDRVLRPAKVKVSLGPVNK 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LB471.2e-8155.69GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE... [more]
Q9FLP32.1e-7556.11GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE... [more]
Q3SW781.4e-2941.21Protein GrpE OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 10... [more]
A4YJR14.0e-2946.45Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1[more]
Q79V151.2e-2844.38Protein GrpE OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / I... [more]
Match NameE-valueIdentityDescription
A0A1S3BLD98.3e-13989.33GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103491087 PE=3 SV=1[more]
A0A5A7UL937.8e-13787.58GrpE protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943... [more]
A0A1S3BKR77.8e-13787.58GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103491087 PE=3 SV=1[more]
A0A0A0L6837.3e-13586.93GrpE protein homolog OS=Cucumis sativus OX=3659 GN=Csa_3G073880 PE=3 SV=1[more]
A0A6J1DCM01.2e-12987.71GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111019268 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038903423.17.2e-14592.81grpE protein homolog 2, mitochondrial-like [Benincasa hispida][more]
XP_008449137.11.7e-13889.33PREDICTED: protein GrpE-like isoform X2 [Cucumis melo][more]
XP_008449136.11.6e-13687.58PREDICTED: protein GrpE-like isoform X1 [Cucumis melo] >KAA0055848.1 protein Grp... [more]
XP_004149344.11.5e-13486.93grpE protein homolog 2, mitochondrial [Cucumis sativus] >KGN56097.1 hypothetical... [more]
XP_022151304.12.5e-12987.71uncharacterized protein LOC111019268 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G26780.18.3e-8355.69Co-chaperone GrpE family protein [more]
AT5G55200.11.5e-7656.11Co-chaperone GrpE family protein [more]
AT5G17710.11.8e-0827.32Co-chaperone GrpE family protein [more]
AT5G17710.21.8e-0827.32Co-chaperone GrpE family protein [more]
AT1G36390.19.1e-0526.20Co-chaperone GrpE family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 115..149
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..95
NoneNo IPR availablePANTHERPTHR21237:SF35GRPE PROTEIN HOMOLOGcoord: 3..283
IPR000740GrpE nucleotide exchange factorPRINTSPR00773GRPEPROTEINcoord: 137..153
score: 38.6
coord: 260..279
score: 52.66
coord: 233..248
score: 45.9
IPR000740GrpE nucleotide exchange factorPFAMPF01025GrpEcoord: 115..281
e-value: 3.7E-48
score: 163.3
IPR000740GrpE nucleotide exchange factorPANTHERPTHR21237GRPE PROTEINcoord: 3..283
IPR000740GrpE nucleotide exchange factorPROSITEPS01071GRPEcoord: 236..279
IPR000740GrpE nucleotide exchange factorHAMAPMF_01151GrpEcoord: 73..281
score: 18.797405
IPR000740GrpE nucleotide exchange factorCDDcd00446GrpEcoord: 130..279
e-value: 3.71823E-59
score: 183.861
IPR009012GrpE nucleotide exchange factor, headGENE3D2.30.22.10Head domain of nucleotide exchange factor GrpEcoord: 225..281
e-value: 9.8E-21
score: 75.5
IPR009012GrpE nucleotide exchange factor, headSUPERFAMILY51064Head domain of nucleotide exchange factor GrpEcoord: 226..281
IPR013805GrpE nucleotide exchange factor, coiled-coilGENE3D3.90.20.20coord: 109..224
e-value: 8.6E-29
score: 101.5
IPR013805GrpE nucleotide exchange factor, coiled-coilSUPERFAMILY58014Coiled-coil domain of nucleotide exchange factor GrpEcoord: 116..225

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G005680.1Lsi05G005680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0001405 PAM complex, Tim23 associated import motor
molecular_function GO:0000774 adenyl-nucleotide exchange factor activity
molecular_function GO:0051087 chaperone binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0051082 unfolded protein binding