Lsi05G005320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G005320
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectin lyase-like superfamily protein, putative
Location	chr05: 6651536 .. 6653980 (-)
RNA-Seq Expression	Lsi05G005320
Synteny	Lsi05G005320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAATTTGGTGCCCAACCTGACGGAATAACGGATAATAGTAAGGTAAAAATTTTTATTTTCAATATAAAATCACATATACTTTAAAATTCAACTCTAATTAATAACTTTTTTAATAGTTTTCAATGTAGTAATTTATAAAATTAGTGACAATAAAAATTGTTATTCATATATATGTTGATAATGTTTTTGTTTCTTGTTTTAATTTTTTTTTAAAAAAAAAAGAAACAAAAGCATTAGAAGCACTAGTATATGTATGTATGAAATTTTTTGCTACCTTTTTAAATTTTCAAAACTTGACTTATACTTCGAAATCTATCATACATAGTTGATTAAAAAAGACAAATAAATCTGAGGTAGATGTAATTATTATTATTATTATTATTATAAAAAGTACATTATCAAATCAGCTCTAATTTATTTCTATCATTTAAGAGACTAAGACATGTCAACATATACTAGACACTCATGGTACATAATGTACTTTTGTTTTATTTTTTTTTAGTTTGAAAATAAAATTTATATATATATATAAAAAAAAAAAAAGATAAATTTCAATGTCATTAAAAATGATTCTCAAAACTATGAAATTTGAAATTTTAAGCTATCATTTTGTTGAAAGATGATGATTATAAAAGGTTAATGAGTTTATTTGTATAATAGGCATTAAGTATGGCATGGAGCCAAGCTTGTGGAAACAATGGAGGTGGTGTAGTTTTAATACCAGCACAAGGGAAATTCTTAGTTTTGCCATTGCTGCTTCAAGGTCCATGCCATGGCTTCATTCGTATACAACTTGACGGCGAATTGTTGGCTCCTGCCGATAAGCATTTCGCCACCGGCAACTATTGGCTTAGTTTCAACCAAGTGAACAACTTGTTCATCGATGGCTTGGGTCGCTTGGACGATCGTGGCTCCACCTCTTGGCCTTACAACTCTGCTAACCGTCCCATCGTAAGTTTATCGATCACTTTTCCTTTCTTTCTCACTAACAATAAGTCTAACATTTGTTTGAAGTAAACAACTCATTTCCATGCATGTATCTGTTTCTATTACAATTAGTACTTTATTTATTTATCTTTTTCTGAATTTTGAAAAAAAAAAGTTATTTGTTATGTTGATAATGAAAATGTTTATATGATTGTTTCCTACTATTATTTTAAATACAACTCCAAAACACACTTGTTGCAAACTAACAATTTGATATTATTTTGTGTAGTCTATAAAACTCAATAATATATGTTGAGCATTAAACTCAAGATAATCAGTTCTTTGGGAGTTTTTCAGCAGGTATTTGAAAGGTCAAACAATATCCAAATTAAATGTGTACTTTCTAATGGTTCAAGTTTCAGGACGTTTGGAGTTTTCTAGAAAAAGTTATGGCAGTTTGAGTAATGAAAGGTCAAGAATTACTGCAACTTTCCAAATTTTATCTTAATTATTGTAATCTCTTTAATTTTGCTTTTATTAAAGGAATTTAGAGAGACACCTTGTGAGACTATATATAGTGATATTGTAACTCTTGCCAAAAGTAAGTTGTGAAAGAAATTGTAGAAGTAAAAGCAATTTACTTTTTACTTTGTTCTTTCTCTCTTCAAAGGTCTCCTCCTTTTGTCAATATCAACATTGTAACAATCAGAAACATAAAATCCTACGATAGCAAACTCTTCCATTTCGCTGTTCATGAATGTCATGACGTCACGTTTGATCGCGTTACCATAATAGCTCCACCAGATAGCCCCAACACAGATGGAATCCACATTTCATCATCGAATGGTATCAACATCATGCATTCCGTGATCGGCACAGGAGACGACTGCATCTCCTTGGGTCCTGGAAGCAAATTCATCAACATAAGCAATGTTCAATGTGGTCCTGGCCATGGAATTAGCATCGGTAGCCTTGGAAAGTACCCAAACAAAGAAGACGTCTTCGAAGTCACAGTAAGAGACTCCACATTCGTTGGCACGTCCAATGGTGTGAGGATCAAATCATGGTCGTCTTCATATTCTAGCATGGTTAGCAAAATCGTATATCTCAATCTCCAAATGAACAATGTCAAAAACCCTATCATCATCGATCAAGAGTATTGTCCTAGCTCTTGCACTCCAAATGAAATGGTATATGAAAGATAATTTGAGAGCCTTATGGTTTTATCAAATGGTATATGTACATATATGATTCTAAAGCAAAATGTGGTTTTATGGTTGGCAGATGAGTAAATCAAGAGTGGAAATTAAAGATGTGAGATATGAAGGCATAAGGGGGAGTTCAAATTCACAAGTGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGTCAAGGCATAGTGTTGCAAGACATTAACCTTCCTTTCAATGGTGGTGGCCAACCAACAACTTCCATTTGTCATAATGTTAGAGGTTCTACTTTTGGCCAACAATTACCTTCCTCTTGCCTTTAG

mRNA sequence

ATGCAATTTGGTGCCCAACCTGACGGAATAACGGATAATAGTAAGGCATTAAGTATGGCATGGAGCCAAGCTTGTGGAAACAATGGAGGTGGTGTAGTTTTAATACCAGCACAAGGGAAATTCTTAGTTTTGCCATTGCTGCTTCAAGGTCCATGCCATGGCTTCATTCGTATACAACTTGACGGCGAATTGTTGGCTCCTGCCGATAAGCATTTCGCCACCGGCAACTATTGGCTTAGTTTCAACCAAGTGAACAACTTGTTCATCGATGGCTTGGGTCGCTTGGACGATCGTGGCTCCACCTCTTGGCCTTACAACTCTGCTAACCGTCCCATCGTAACTCCACCAGATAGCCCCAACACAGATGGAATCCACATTTCATCATCGAATGGTATCAACATCATGCATTCCGTGATCGGCACAGGAGACGACTGCATCTCCTTGGGTCCTGGAAGCAAATTCATCAACATAAGCAATGTTCAATGTGGTCCTGGCCATGGAATTAGCATCGGTAGCCTTGGAAAGTACCCAAACAAAGAAGACGTCTTCGAAGTCACAGTAAGAGACTCCACATTCGTTGGCACGTCCAATGGTGTGAGGATCAAATCATGGTCGTCTTCATATTCTAGCATGGTTAGCAAAATCGTATATCTCAATCTCCAAATGAACAATGTCAAAAACCCTATCATCATCGATCAAGACAAAATGTGGTTTTATGGTTGGCAGATGAGTAAATCAAGAGTGGAAATTAAAGATGTGAGATATGAAGGCATAAGGGGGAGTTCAAATTCACAAGTGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGTCAAGGCATAGTGTTGCAAGACATTAACCTTCCTTTCAATGGTGGTGGCCAACCAACAACTTCCATTTGTCATAATGTTAGAGGTTCTACTTTTGGCCAACAATTACCTTCCTCTTGCCTTTAG

Coding sequence (CDS)

ATGCAATTTGGTGCCCAACCTGACGGAATAACGGATAATAGTAAGGCATTAAGTATGGCATGGAGCCAAGCTTGTGGAAACAATGGAGGTGGTGTAGTTTTAATACCAGCACAAGGGAAATTCTTAGTTTTGCCATTGCTGCTTCAAGGTCCATGCCATGGCTTCATTCGTATACAACTTGACGGCGAATTGTTGGCTCCTGCCGATAAGCATTTCGCCACCGGCAACTATTGGCTTAGTTTCAACCAAGTGAACAACTTGTTCATCGATGGCTTGGGTCGCTTGGACGATCGTGGCTCCACCTCTTGGCCTTACAACTCTGCTAACCGTCCCATCGTAACTCCACCAGATAGCCCCAACACAGATGGAATCCACATTTCATCATCGAATGGTATCAACATCATGCATTCCGTGATCGGCACAGGAGACGACTGCATCTCCTTGGGTCCTGGAAGCAAATTCATCAACATAAGCAATGTTCAATGTGGTCCTGGCCATGGAATTAGCATCGGTAGCCTTGGAAAGTACCCAAACAAAGAAGACGTCTTCGAAGTCACAGTAAGAGACTCCACATTCGTTGGCACGTCCAATGGTGTGAGGATCAAATCATGGTCGTCTTCATATTCTAGCATGGTTAGCAAAATCGTATATCTCAATCTCCAAATGAACAATGTCAAAAACCCTATCATCATCGATCAAGACAAAATGTGGTTTTATGGTTGGCAGATGAGTAAATCAAGAGTGGAAATTAAAGATGTGAGATATGAAGGCATAAGGGGGAGTTCAAATTCACAAGTGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGTCAAGGCATAGTGTTGCAAGACATTAACCTTCCTTTCAATGGTGGTGGCCAACCAACAACTTCCATTTGTCATAATGTTAGAGGTTCTACTTTTGGCCAACAATTACCTTCCTCTTGCCTTTAG

Protein sequence

MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLDGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPIVTPPDSPNTDGIHISSSNGINIMHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVRIKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGWQMSKSRVEIKDVRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL

Homology

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BLAST of Lsi05G005320 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.1e-65
Identity = 137/362 (37.85%), Postives = 199/362 (54.97%), Query Frame = 0


Query: 2   QFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLD 61
           + GA  +G TD++KA+  AW+ ACG  G   +LIP +G FLV PL   GPC G + IQ++
Sbjct: 45  KLGASGNGKTDSTKAVQEAWASACGGTGKQTILIP-KGDFLVGPLNFTGPCKGDVTIQVN 104

Query: 62  GELLAPAD-KHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRP--------- 121
           G LLA  D   +     W+   +V+NL I G G+LD +G   W  NS  +          
Sbjct: 105 GNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKILPNS 164

Query: 122 --------------------------------------IVTPPDSPNTDGIHISSSNGIN 181
                                                 +  P DSPNTDGIH+  S+G+ 
Sbjct: 165 LVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVT 224

Query: 182 IMHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFV 241
           I ++VIG GDDCIS+GPG+  +NI+ V CGPGHGISIGSLG+Y +++DV ++ V+D T  
Sbjct: 225 ITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLK 284

Query: 242 GTSNGVRIKSWSSSYSSM-VSKIVYLNLQMNNVKNPIIIDQ----DKMWFYGWQMSKSRV 301
            T+NGVRIK++  + S +  SKI Y N++M +   PIIID     +K+         S+V
Sbjct: 285 KTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTAN---GASKV 344

Query: 302 EIKDVRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGST 311
            +KDV ++ I G+S++  AVN  C+   PC G+ + D+N+ ++G    T ++C N +GS 
Sbjct: 345 TVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSA 402

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BLAST of Lsi05G005320 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 134/363 (36.91%), Postives = 202/363 (55.65%), Query Frame = 0


Query: 3   FGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGF-IRIQLD 62
           +GA+  G  D S+A+  AW  AC + G   VLIP +G + +  + +QGPC G  I  Q+D
Sbjct: 15  YGAK--GAGDISQAVMKAWKAACASQGPSTVLIP-KGNYNMGEVAMQGPCKGSKIGFQID 74

Query: 63  GELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANR----------- 122
           G + APAD      + W+SF +++ L + G G LD +G T+W  N+ ++           
Sbjct: 75  GVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNL 134

Query: 123 ------------------------------------PIVTPPDSPNTDGIHISSSNGINI 182
                                                +  P  S NTDGIH+  S G+ I
Sbjct: 135 RFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTI 194

Query: 183 MHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVG 242
            ++ I TGDDCIS+GPGS+ + I+ V CGPGHGISIGSLG+Y N+++V  +TV+  TF G
Sbjct: 195 TNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSG 254

Query: 243 TSNGVRIKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGW--QMSKSRVEIKD 302
           T NGVR+K+W +S     + + + +L MNNV+NP+I+DQ+    YG   + + SR+++ +
Sbjct: 255 TMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQE-YCPYGQCSRQAPSRIKLSN 314

Query: 303 VRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQ 316
           + +  IRG+S  +VAV   CS   PC  + + +INL + G G P TS C NV+ +  G+Q
Sbjct: 315 INFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQ 373

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BLAST of Lsi05G005320 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.1e-64
Identity = 139/362 (38.40%), Postives = 197/362 (54.42%), Query Frame = 0


Query: 2   QFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLD 61
           + GA  +G TD++KA+  AW+ ACG  G   +LIP +G FLV  L   GPC G + IQ+D
Sbjct: 45  KLGASGNGKTDSTKAVQEAWASACGGTGKQTILIP-KGDFLVGQLNFTGPCKGDVTIQVD 104

Query: 62  GELLAPAD-KHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANR---------- 121
           G LLA  D   +     W+   +V+NL I G G LD +G   W  NS  +          
Sbjct: 105 GNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNS 164

Query: 122 -------------------------------------PIVTPPDSPNTDGIHISSSNGIN 181
                                                 +  P DSPNTDGIH+  S+GI 
Sbjct: 165 LVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGIT 224

Query: 182 IMHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFV 241
           I ++VIG GDDCIS+GPG+  +NI+ V CGPGHGISIGSLG+Y +++DV ++ V+D T  
Sbjct: 225 ITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLK 284

Query: 242 GTSNGVRIKSWSSSYSSM-VSKIVYLNLQMNNVKNPIIIDQ----DKMWFYGWQMSKSRV 301
            T  GVRIK++  + S + VSKI Y N++M +  NPI ID     +K+         S+V
Sbjct: 285 KTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTAN---GASKV 344

Query: 302 EIKDVRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGST 311
            +KDV ++ I G+S++  AV+  C+   PC G+ + D+N+ ++G    T +IC N +GST
Sbjct: 345 TVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAKGST 402

[Close]

BLAST of Lsi05G005320 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 8.9e-64
Identity = 135/355 (38.03%), Postives = 199/355 (56.06%), Query Frame = 0


Query: 12  DNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLDGELLAPADKH 71
           D+++AL+ AW +AC +     +L+P +G F V  + L+GPC   I +QL G L APAD  
Sbjct: 1   DSTQALTTAWKEACASASPSTILVP-KGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPS 60

Query: 72  FATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSA--NRPI----------------- 131
              G  W++ N+++ L I G G  D +G ++W  N    N PI                 
Sbjct: 61  KIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSI 120

Query: 132 ---VTPPDSP--------------------------NTDGIHISSSNGINIMHSVIGTGD 191
              VT  DS                           NTDGIHI  S+G+NI+++ I TGD
Sbjct: 121 LRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGD 180

Query: 192 DCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVRIKS 251
           DCISLG GSK INI+N+ CGPGHGIS+GSLG+Y N+E V  + V++ T  G+ NGVRIK+
Sbjct: 181 DCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKT 240

Query: 252 WSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGWQMS-KSRVEIKDVRYEGIRGSS 311
           W  S     S++ + ++ MN+V  PI+IDQ    +        S V++  + ++ I+G+S
Sbjct: 241 WPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 312 NSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSC 318
            ++ AV   CS+ FPC G+ L DI+L ++G G P TS+C N++ +  G+Q+P+ C
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAIC 354

[Close]

BLAST of Lsi05G005320 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 138/362 (38.12%), Postives = 196/362 (54.14%), Query Frame = 0


Query: 2   QFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLD 61
           + GA  +G TD++KA+  AW+ ACG  G   +LIP +G FLV  L   GPC G + IQ+D
Sbjct: 45  KLGASGNGKTDSTKAVQEAWASACGGTGKQTILIP-KGDFLVGQLNFTGPCKGDVTIQVD 104

Query: 62  GELLAPAD-KHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANR---------- 121
           G LLA  D   +     W+   +V+NL I G G LD +G   W  NS  +          
Sbjct: 105 GNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNS 164

Query: 122 -------------------------------------PIVTPPDSPNTDGIHISSSNGIN 181
                                                 +  P DSPNTDGIH+  S+GI 
Sbjct: 165 LVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHMGDSSGIT 224

Query: 182 IMHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFV 241
           I ++VIG GDDCIS+GPG+  +NI+ V CGPGHGISIGSLG+Y +++DV ++ V+D T  
Sbjct: 225 ITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLK 284

Query: 242 GTSNGVRIKSWSSSYSSM-VSKIVYLNLQMNNVKNPIIIDQ----DKMWFYGWQMSKSRV 301
            T  GVRIK++  + S + VSKI Y N++M +  NPI ID     +K+         S+V
Sbjct: 285 KTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTAN---GASKV 344

Query: 302 EIKDVRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGST 311
            +KDV ++ I G+S++  A++  C+   PC G  + D+N+ ++G    T +IC N +GST
Sbjct: 345 TVKDVTFKNITGTSSTPEAISLLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNAKGST 402

[Close]

BLAST of Lsi05G005320 vs. ExPASy TrEMBL
Match: A0A0A0L7H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.3e-145
Identity = 264/361 (73.13%), Postives = 290/361 (80.33%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           M FGA+PD ITDNSKALS AWS+AC NNGGG+VLIPA+G+FLVLPLLLQGPCHGFIRIQL
Sbjct: 1   MHFGAKPDAITDNSKALSSAWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQL 60

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRP--------- 120
           DGELLAP D+HFATG+YWLS +QVNNLFIDGLG LD RGST+WP  S NRP         
Sbjct: 61  DGELLAPLDEHFATGDYWLSIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGIN 120

Query: 121 -------------------------------IVTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                          ++ P +SPNTDGIHISSS+GINIMHS IG
Sbjct: 121 NARITNIKSYNSKLFHFAMHGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIG 180

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+N+QCGPGHGISIGSLGKYPN+EDVFEVTVRDSTF+GTSNGVR
Sbjct: 181 TGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVR 240

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKM-WFYGWQ--MSKSRVEIKDVRYEG 300
           IK+WSSSYSSMVSK+ +LNLQMNNVKNPIIIDQ+      G Q  MSKS V+IKDVRYEG
Sbjct: 241 IKTWSSSYSSMVSKVTFLNLQMNNVKNPIIIDQNYCPGSCGSQKMMSKSMVQIKDVRYEG 300

Query: 301 IRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSC 319
           IRGSSN+QVAV+FECS+V PCQGIVLQDINLPFNGGG+ TTS CHNVRGS FG QLP SC
Sbjct: 301 IRGSSNTQVAVDFECSEVVPCQGIVLQDINLPFNGGGR-TTSNCHNVRGSAFGHQLPPSC 360

[Close]

BLAST of Lsi05G005320 vs. ExPASy TrEMBL
Match: A0A5D3B9J1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001860 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.8e-139
Identity = 256/360 (71.11%), Postives = 286/360 (79.44%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           MQFGA+PD ITDNSKALS AWS+AC NNGGGVVLIPA G+FLVLPLLLQGPCHGFI IQL
Sbjct: 1   MQFGAKPDAITDNSKALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQL 60

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPI-------- 120
           DGELLAP +++  TG+YWLS ++VNNLFIDGLGRL+ RGST+WP  S NRPI        
Sbjct: 61  DGELLAPLNEN--TGDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGIN 120

Query: 121 --------------------------------VTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                           + P +SPNTDGIH+S S+GINIMHS IG
Sbjct: 121 NARISNIKSYNSKLFHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIG 180

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+NVQCGPGHGISIGSLGKYPN+EDVFEVTVRDSTF+GT+NG R
Sbjct: 181 TGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGAR 240

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGW--QMSKSRVEIKDVRYEGI 300
           IK+WSSSYS+MVSKI +LNLQMNNVKNPIIIDQ          +MS+S+V+IKDVRYEGI
Sbjct: 241 IKTWSSSYSNMVSKITFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGI 300

Query: 301 RGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           RGSSN+QVAV+FECSQVFPCQGIVLQDINLPFNGGG+ TT+ CHNVRGS FG QLP SCL
Sbjct: 301 RGSSNTQVAVDFECSQVFPCQGIVLQDINLPFNGGGR-TTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of Lsi05G005320 vs. ExPASy TrEMBL
Match: A0A1S4DX91 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103491147 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 255/360 (70.83%), Postives = 286/360 (79.44%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           MQFGA+PD ITDNSKALS AWS+AC NNGGGVVLIPA G+FLVLPLLLQGPCHGFI IQL
Sbjct: 1   MQFGAKPDAITDNSKALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQL 60

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPI-------- 120
           DGELLAP +++  TG+YWLS ++VNNLFIDGLGRL+ RGST+WP  S NRPI        
Sbjct: 61  DGELLAPLNEN--TGDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGIN 120

Query: 121 --------------------------------VTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                           + P +SPNTDGIH+S S+GINIMHS IG
Sbjct: 121 NARISNIKSYNSKLFHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIG 180

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+NVQCGPGHGISIGSLGKYPN+EDVFEVTVRDSTF+GT+NG R
Sbjct: 181 TGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGAR 240

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGW--QMSKSRVEIKDVRYEGI 300
           IK+WSSSYS+MVSK+ +LNLQMNNVKNPIIIDQ          +MS+S+V+IKDVRYEGI
Sbjct: 241 IKTWSSSYSNMVSKVTFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGI 300

Query: 301 RGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           RGSSN+QVAV+FECSQVFPCQGIVLQDINLPFNGGG+ TT+ CHNVRGS FG QLP SCL
Sbjct: 301 RGSSNTQVAVDFECSQVFPCQGIVLQDINLPFNGGGR-TTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of Lsi05G005320 vs. ExPASy TrEMBL
Match: A0A5D3BB58 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001870 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.6e-132
Identity = 249/351 (70.94%), Postives = 271/351 (77.21%), Query Frame = 0


Query: 16  ALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLDGELLAPADKHFATG 75
           ALS AWS AC NNGGGVVLIPAQG+FLVLPLLLQGPCHG IRIQLDGELLAP+DK FATG
Sbjct: 2   ALSSAWSGACENNGGGVVLIPAQGRFLVLPLLLQGPCHGVIRIQLDGELLAPSDKQFATG 61

Query: 76  NYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPI----------------------- 135
           +YWLS   VNNLFIDGLGRL   GS++WP +SANRPI                       
Sbjct: 62  DYWLSIQNVNNLFIDGLGRLTGSGSSAWPSHSANRPIVRFHLSSMKLNNINTASIKNISS 121

Query: 136 -----------------------VTPPDSPNTDGIHISSSNGINIMHSVIGTGDDCISLG 195
                                  V P DSPNTDGIHISSS+GINIMHS IGTGDDCISLG
Sbjct: 122 YDSKLFHFAVHGSRDVTFDHVTVVAPGDSPNTDGIHISSSSGINIMHSTIGTGDDCISLG 181

Query: 196 PGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVRIKSWSSSYS 255
           PGSK INI+NV CGPGHGISIGSLGKYPN+E+VFEVTVRDSTFVGT+NG RIKSWSSSYS
Sbjct: 182 PGSKSINITNVYCGPGHGISIGSLGKYPNEENVFEVTVRDSTFVGTTNGARIKSWSSSYS 241

Query: 256 SMVSKIVYLNLQMNNVKNPIIIDQDKM--WFYGWQMSKSRVEIKDVRYEGIRGSSNSQVA 315
           SMVSK+ ++NLQMNNVKNPIIIDQ          +MSKS+V+I+DVRYEGIRGSSN+QVA
Sbjct: 242 SMVSKVTFVNLQMNNVKNPIIIDQSYCPDSCGPNEMSKSKVQIEDVRYEGIRGSSNTQVA 301

Query: 316 VNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           V+FECSQV PCQGIVLQDINL FNGGGQ +TSICHNV+GS FGQQLP SCL
Sbjct: 302 VDFECSQVLPCQGIVLQDINLTFNGGGQ-STSICHNVKGSAFGQQLPPSCL 351

[Close]

BLAST of Lsi05G005320 vs. ExPASy TrEMBL
Match: A0A6J1F153 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.3e-117
Identity = 221/366 (60.38%), Postives = 267/366 (72.95%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           +QFGA  DG T+++KALS AW +ACG+NG GVVL+PA G+FL+LPL+L+GPC   I + +
Sbjct: 26  IQFGATADGKTESTKALSEAWREACGHNGHGVVLVPA-GRFLILPLVLEGPCKVSIGLLI 85

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRP--------- 120
            GELLAP  K+FATG YWLS ++V+NLF+DG G L+  GS++WP +++NRP         
Sbjct: 86  HGELLAPVSKNFATGGYWLSISKVDNLFVDGSGSLNGGGSSAWPSDTSNRPVSMKLSSVN 145

Query: 121 -------------------------------IVTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                          ++ P DSPNTDGIH+S S+GINIMHSVI 
Sbjct: 146 TARISKITSSNSKFFHFGVHGCRDVTFDHVTVIAPADSPNTDGIHVSKSSGINIMHSVIR 205

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+++QCGPGHGISIGSLGKYPN+EDV EVT+RDSTFVGT NGVR
Sbjct: 206 TGDDCISLGPGSKFINITDIQCGPGHGISIGSLGKYPNEEDVSEVTIRDSTFVGTDNGVR 265

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQ--------DKMWFYGWQMSKSRVEIKD 300
           IK+WSSS+SS VSKI +LNL+M +VKNPIIIDQ        DK       MS S+V+I+D
Sbjct: 266 IKTWSSSFSSFVSKIAFLNLEMKDVKNPIIIDQKYCPDCKEDK-------MSNSKVQIRD 325

Query: 301 VRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQ 319
           VRYE IRGSS SQVAV+FECSQV  C+ IVLQDINL + G GQP TSICHNV+GS  G+Q
Sbjct: 326 VRYEDIRGSSTSQVAVSFECSQVCSCESIVLQDINLSYKGDGQP-TSICHNVQGSASGKQ 382

[Close]

BLAST of Lsi05G005320 vs. NCBI nr
Match: XP_038904214.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 530.4 bits (1365), Expect = 1.1e-146
Identity = 263/358 (73.46%), Postives = 290/358 (81.01%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           MQFGA+PD ITDNSKALS AWS+ACGN+GGGVVLIPAQG+FLVLPLLLQGPCHGFI IQL
Sbjct: 34  MQFGAKPDAITDNSKALSNAWSEACGNSGGGVVLIPAQGRFLVLPLLLQGPCHGFIHIQL 93

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRP--------- 120
           DGELLAP +K FATG+YWLS NQVNNLFIDGLGRL+ RGST+WPY+SA+RP         
Sbjct: 94  DGELLAPVNKQFATGDYWLSINQVNNLFIDGLGRLNGRGSTAWPYSSASRPVSMKLTNIN 153

Query: 121 -------------------------------IVTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                          ++ P DSPNTDGIHISSS+GINIMHSVI 
Sbjct: 154 TARIRNIKSYNSKLFHFAVHGCHNVMFDHVTVIAPTDSPNTDGIHISSSSGINIMHSVIS 213

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+N+QCGPGHGISIGSLGKYPN+EDVFEVT+RDS F+GTSNGVR
Sbjct: 214 TGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPNEEDVFEVTIRDSIFIGTSNGVR 273

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGWQMSKSRVEIKDVRYEGIRG 300
           IKSWSSSYSS+VSK+ Y NLQMNNVKNPI+IDQ            SRV+IKDVRYEGIRG
Sbjct: 274 IKSWSSSYSSLVSKVSYRNLQMNNVKNPILIDQMYCPDSCGPNEMSRVQIKDVRYEGIRG 333

Query: 301 SSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           +SN+QVAVNFECSQ+FPCQGIVLQDINLPFNGGG+ +TSICHNVRGS FGQQLP SCL
Sbjct: 334 TSNTQVAVNFECSQIFPCQGIVLQDINLPFNGGGK-STSICHNVRGSAFGQQLPPSCL 390

[Close]

BLAST of Lsi05G005320 vs. NCBI nr
Match: XP_031738767.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 264/361 (73.13%), Postives = 290/361 (80.33%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           M FGA+PD ITDNSKALS AWS+AC NNGGG+VLIPA+G+FLVLPLLLQGPCHGFIRIQL
Sbjct: 1   MHFGAKPDAITDNSKALSSAWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQL 60

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRP--------- 120
           DGELLAP D+HFATG+YWLS +QVNNLFIDGLG LD RGST+WP  S NRP         
Sbjct: 61  DGELLAPLDEHFATGDYWLSIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGIN 120

Query: 121 -------------------------------IVTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                          ++ P +SPNTDGIHISSS+GINIMHS IG
Sbjct: 121 NARITNIKSYNSKLFHFAMHGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIG 180

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+N+QCGPGHGISIGSLGKYPN+EDVFEVTVRDSTF+GTSNGVR
Sbjct: 181 TGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVR 240

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKM-WFYGWQ--MSKSRVEIKDVRYEG 300
           IK+WSSSYSSMVSK+ +LNLQMNNVKNPIIIDQ+      G Q  MSKS V+IKDVRYEG
Sbjct: 241 IKTWSSSYSSMVSKVTFLNLQMNNVKNPIIIDQNYCPGSCGSQKMMSKSMVQIKDVRYEG 300

Query: 301 IRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSC 319
           IRGSSN+QVAV+FECS+V PCQGIVLQDINLPFNGGG+ TTS CHNVRGS FG QLP SC
Sbjct: 301 IRGSSNTQVAVDFECSEVVPCQGIVLQDINLPFNGGGR-TTSNCHNVRGSAFGHQLPPSC 360

[Close]

BLAST of Lsi05G005320 vs. NCBI nr
Match: KAA0065793.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96532.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 503.1 bits (1294), Expect = 1.8e-138
Identity = 256/360 (71.11%), Postives = 286/360 (79.44%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           MQFGA+PD ITDNSKALS AWS+AC NNGGGVVLIPA G+FLVLPLLLQGPCHGFI IQL
Sbjct: 1   MQFGAKPDAITDNSKALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQL 60

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPI-------- 120
           DGELLAP +++  TG+YWLS ++VNNLFIDGLGRL+ RGST+WP  S NRPI        
Sbjct: 61  DGELLAPLNEN--TGDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGIN 120

Query: 121 --------------------------------VTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                           + P +SPNTDGIH+S S+GINIMHS IG
Sbjct: 121 NARISNIKSYNSKLFHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIG 180

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+NVQCGPGHGISIGSLGKYPN+EDVFEVTVRDSTF+GT+NG R
Sbjct: 181 TGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGAR 240

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGW--QMSKSRVEIKDVRYEGI 300
           IK+WSSSYS+MVSKI +LNLQMNNVKNPIIIDQ          +MS+S+V+IKDVRYEGI
Sbjct: 241 IKTWSSSYSNMVSKITFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGI 300

Query: 301 RGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           RGSSN+QVAV+FECSQVFPCQGIVLQDINLPFNGGG+ TT+ CHNVRGS FG QLP SCL
Sbjct: 301 RGSSNTQVAVDFECSQVFPCQGIVLQDINLPFNGGGR-TTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of Lsi05G005320 vs. NCBI nr
Match: XP_038904218.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 502.7 bits (1293), Expect = 2.4e-138
Identity = 255/347 (73.49%), Postives = 276/347 (79.54%), Query Frame = 0


Query: 16  ALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLDGELLAPADKHFATG 75
           ALS AWS+ACGNNGGGVVLIPA G+FLVLPLLLQGPCHGFIRIQ DGELLAP D HFATG
Sbjct: 61  ALSRAWSEACGNNGGGVVLIPALGRFLVLPLLLQGPCHGFIRIQFDGELLAPTDNHFATG 120

Query: 76  NYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPI----------------------- 135
           NYWLS NQVNNLFIDGLGRL+ RGST+W Y SANRPI                       
Sbjct: 121 NYWLSINQVNNLFIDGLGRLNGRGSTAWYYKSANRPISMKLNNINTARIRNIKSYNSKLF 180

Query: 136 -----------------VTPPDSPNTDGIHISSSNGINIMHSVIGTGDDCISLGPGSKFI 195
                            + P +SPNTDGIHISSS+GINIMHS+IGTGDDCISLGPGSKFI
Sbjct: 181 HFAMHGCHDVTFDHVTMIAPANSPNTDGIHISSSSGINIMHSMIGTGDDCISLGPGSKFI 240

Query: 196 NISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVRIKSWSSSYSSMVSKI 255
           NI+NV+CGPGHGISIGSLGKYPN+ED+FEVTVRDSTF+GTSNGVRIKSW SSYSSMVSK+
Sbjct: 241 NITNVRCGPGHGISIGSLGKYPNEEDIFEVTVRDSTFIGTSNGVRIKSWLSSYSSMVSKV 300

Query: 256 VYLNLQMNNVKNPIIIDQDKMWFYGW----QMSKSRVEIKDVRYEGIRGSSNSQVAVNFE 315
            Y+NLQMNNVKNP+ IDQ  M+         MSKSRV IKDVRYEGIRG+SN+QVAVNFE
Sbjct: 301 TYMNLQMNNVKNPVPIDQ--MYCPDSCGPNAMSKSRVHIKDVRYEGIRGTSNTQVAVNFE 360

Query: 316 CSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           CSQVFPC+GI LQDINLPFNGGG  +TS CHNVRGSTFGQQLP SCL
Sbjct: 361 CSQVFPCEGIGLQDINLPFNGGGN-STSRCHNVRGSTFGQQLPPSCL 404

[Close]

BLAST of Lsi05G005320 vs. NCBI nr
Match: XP_016900599.1 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 502.7 bits (1293), Expect = 2.4e-138
Identity = 255/360 (70.83%), Postives = 286/360 (79.44%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           MQFGA+PD ITDNSKALS AWS+AC NNGGGVVLIPA G+FLVLPLLLQGPCHGFI IQL
Sbjct: 1   MQFGAKPDAITDNSKALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQL 60

Query: 61  DGELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPI-------- 120
           DGELLAP +++  TG+YWLS ++VNNLFIDGLGRL+ RGST+WP  S NRPI        
Sbjct: 61  DGELLAPLNEN--TGDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGIN 120

Query: 121 --------------------------------VTPPDSPNTDGIHISSSNGINIMHSVIG 180
                                           + P +SPNTDGIH+S S+GINIMHS IG
Sbjct: 121 NARISNIKSYNSKLFHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIG 180

Query: 181 TGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTSNGVR 240
           TGDDCISLGPGSKFINI+NVQCGPGHGISIGSLGKYPN+EDVFEVTVRDSTF+GT+NG R
Sbjct: 181 TGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGAR 240

Query: 241 IKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKMWFYGW--QMSKSRVEIKDVRYEGI 300
           IK+WSSSYS+MVSK+ +LNLQMNNVKNPIIIDQ          +MS+S+V+IKDVRYEGI
Sbjct: 241 IKTWSSSYSNMVSKVTFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGI 300

Query: 301 RGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQLPSSCL 319
           RGSSN+QVAV+FECSQVFPCQGIVLQDINLPFNGGG+ TT+ CHNVRGS FG QLP SCL
Sbjct: 301 RGSSNTQVAVDFECSQVFPCQGIVLQDINLPFNGGGR-TTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of Lsi05G005320 vs. TAIR 10
Match: AT1G43080.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.9e-67
Identity = 141/369 (38.21%), Postives = 204/369 (55.28%), Query Frame = 0


Query: 2   QFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLD 61
           ++G++PDG TDN+ A +  W +AC    G   +   +G F +  +   GPC   I   +D
Sbjct: 35  RYGSRPDGKTDNANAFTSVWKRACTRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFVID 94

Query: 62  GELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANR----------- 121
           G LLAPA+      + W++F  +NNL I G G LD +G  SWP N  ++           
Sbjct: 95  GTLLAPANPRDIKQDTWINFRYINNLSISGSGTLDGQGKYSWPLNDCHKNTNCPKLAMTM 154

Query: 122 ------------------------------------PIVTPPDSPNTDGIHISSSNGINI 181
                                                I  P DSPNTDGI + S + I+I
Sbjct: 155 GFAFVNNSRIKDITSLNSKMGHFNFFSVHRFNITGVTITAPGDSPNTDGIKMGSCSNIHI 214

Query: 182 MHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVG 241
            ++ IGTGDDCI++  G+  ++ISN++CGPGHGIS+GSLGK  +++DV  +TVRD+ F G
Sbjct: 215 SNTNIGTGDDCIAILSGTTNLDISNIKCGPGHGISVGSLGKNKDEKDVKHLTVRDTVFNG 274

Query: 242 TSNGVRIKSWSSSYSS-MVSKIVYLNLQMNNVKNPIIIDQDKMWFYGWQMSK---SRVEI 301
           TS+G+RIK+W SS S  +VS  +Y N+QM +V  PI IDQ        +  K   S V+I
Sbjct: 275 TSDGIRIKTWESSASKIVVSNFIYENIQMIDVGKPINIDQKYCPHPPCEHEKKGESHVQI 334

Query: 302 KDVRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQ-PTTSICHNVRGSTF 319
           +D++ + I G+SN+ VAVN +CS+ FPC+ + L DINL   G  +  +T++C NV GS  
Sbjct: 335 QDIKLKNIYGTSNNIVAVNLQCSKSFPCKNVELIDINLKHTGLEKGHSTAMCENVDGSVR 394

[Close]

BLAST of Lsi05G005320 vs. TAIR 10
Match: AT2G15450.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.4e-66
Identity = 143/367 (38.96%), Postives = 199/367 (54.22%), Query Frame = 0


Query: 4   GAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLDGE 63
           GA+PDG TDN+ A +  WS+AC    G   +   +G F +  +   GPC   I   +DG 
Sbjct: 37  GAKPDGKTDNANAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFIIDGT 96

Query: 64  LLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANR------------- 123
           LLAPA+      + W++F  +NNL I G G LD +G  SWP+N  +              
Sbjct: 97  LLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCPKLAMTMGF 156

Query: 124 ----------------------------------PIVTPPDSPNTDGIHISSSNGINIMH 183
                                              I  P DSPNTDGI + S + I I  
Sbjct: 157 AFVNNSNIKDITSLNSKMGHFNFFSVHHFNITGVTITAPGDSPNTDGIKMGSCSNIQISD 216

Query: 184 SVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTS 243
           + IGTGDDCI++  G+  +NISNV CGPGHGIS+GSLGK  +++DV ++ VRD  F GTS
Sbjct: 217 TNIGTGDDCIAILSGTTNLNISNVNCGPGHGISVGSLGKNKDEKDVKDLIVRDVIFNGTS 276

Query: 244 NGVRIKSWSSSYSS-MVSKIVYLNLQMNNVKNPIIIDQDKMWFYGWQ---MSKSRVEIKD 303
           +G+RIK+W SS S  +VS  VY N+QM +V  PI IDQ        +     +S V+I++
Sbjct: 277 DGIRIKNWESSASKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERKGESHVQIQN 336

Query: 304 VRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNG-GGQPTTSICHNVRGSTFGQ 319
           ++ + I G+S ++VAVN +CS++FPC+ + L DIN+  NG     +TS+C NV G   G+
Sbjct: 337 LKLKNIYGTSKNKVAVNLQCSKIFPCKNVELIDINIKQNGVKDGSSTSVCENVDGFARGK 396

[Close]

BLAST of Lsi05G005320 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.7e-65
Identity = 140/365 (38.36%), Postives = 196/365 (53.70%), Query Frame = 0


Query: 1   MQFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQL 60
           + FGA+PDG TD++KA   AW  AC +     V +P +G FL+ P+  +GPC   I  Q+
Sbjct: 32  VSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVTVP-RGSFLLKPVEFRGPCRSRITFQI 91

Query: 61  DGELLAPAD-KHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWP--------------- 120
            G ++AP+D +      YW+ F +VN + I G G LD RG++ W                
Sbjct: 92  YGTIVAPSDYRGLGNSGYWILFVKVNRISIIG-GTLDARGASFWACRKSGKSCPVGARSM 151

Query: 121 ------------------------YNSANRPI------VTPPDSPNTDGIHISSSNGINI 180
                                    NS N  I      V P  SPNTDG+H+  S G+ +
Sbjct: 152 TFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRKVKLVAPDQSPNTDGLHVQGSAGVTV 211

Query: 181 MHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVG 240
                 TGDDCIS+GPG++ + +S + CGPGHGISIGSLG+  N+  V  +T+ +S F G
Sbjct: 212 TDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGISIGSLGRDANEAGVENITLINSVFSG 271

Query: 241 TSNGVRIKSWSSSYSSMVSKIVYLNLQMNNVKNPIIIDQDKM-WFYGWQMSKSRVEIKDV 300
           + NGVRIK+W+   +  V  +++ NL M NV+NPII+DQ+      G     S V+I  V
Sbjct: 272 SDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPIIVDQNYCPSNQGCPKQGSGVKISQV 331

Query: 301 RYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQPTTSICHNVRGSTFGQQL 319
            Y  I+G+S +Q A+ F+CS+  PCQ I L DI L FN  G+  TS C N++G   G  +
Sbjct: 332 VYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFN--GRSATSTCKNIKGVKAGVVM 391

[Close]

BLAST of Lsi05G005320 vs. TAIR 10
Match: AT1G43090.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.2e-65
Identity = 140/362 (38.67%), Postives = 200/362 (55.25%), Query Frame = 0


Query: 2   QFGAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLD 61
           ++G++PDG TDN+ A +  W  AC    G   +   +G F +  +   GPC   I   +D
Sbjct: 35  RYGSRPDGKTDNANAFTSVWKHACTRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFVID 94

Query: 62  GELLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANR----------- 121
           G LLAPA+      + W++F  +NNL I G G LD +G  SWP N  ++           
Sbjct: 95  GTLLAPANPRDIKQDTWINFRYINNLSISGSGTLDGQGKYSWPLNDCHKNTNCPKLAMTM 154

Query: 122 ------------------------------------PIVTPPDSPNTDGIHISSSNGINI 181
                                                I  P DSPNTDGI + S + I+I
Sbjct: 155 GFAFVNNSRIKDITSLNSKMGHFNFFSVHRFNITGVTITAPGDSPNTDGIKMGSCSNIHI 214

Query: 182 MHSVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVG 241
            ++ IGTGDDCI++  G+  ++ISNV+CGPGHGIS+GSLGK  +++DV  +TVRD+ F G
Sbjct: 215 SNTNIGTGDDCIAILSGTTNLDISNVKCGPGHGISVGSLGKNKDEKDVKHLTVRDTVFNG 274

Query: 242 TSNGVRIKSWSSSYSSM-VSKIVYLNLQMNNVKNPIIIDQDKMWFYGWQ---MSKSRVEI 301
           TS+G+RIK+W SS S + VS  +Y N+QM +V  PI IDQ        +     KS V+I
Sbjct: 275 TSDGIRIKTWESSASKLVVSNFIYENIQMIDVGKPINIDQKYCPHPPCEHEKKGKSHVQI 334

Query: 302 KDVRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNGGGQ-PTTSICHNVRGSTF 312
           +D++ + I G+SN+ VAVN +CS+ FPC+ + L DINL   G  +  +T++C NV GS  
Sbjct: 335 QDIKLKNIYGTSNNIVAVNLQCSKSFPCKNVELIDINLKHTGLEKGHSTAMCENVDGSVR 394

[Close]

BLAST of Lsi05G005320 vs. TAIR 10
Match: AT2G40310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.2e-65
Identity = 140/367 (38.15%), Postives = 201/367 (54.77%), Query Frame = 0


Query: 4   GAQPDGITDNSKALSMAWSQACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGFIRIQLDGE 63
           G++PDG TDN+   +  WS+AC    G   +   +G F +  +   GPC   I   +DG 
Sbjct: 37  GSKPDGKTDNANVFTSIWSRACKRKSGSSKIYVPKGIFYLGGVEFVGPCKNPIEFVIDGT 96

Query: 64  LLAPADKHFATGNYWLSFNQVNNLFIDGLGRLDDRGSTSWPYNSANRPIVTPP------- 123
           LLAPA+      + W++F  +NNL I G G LD +G  SWP N  ++ +  P        
Sbjct: 97  LLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPLNDCHKNLNCPKLAMTMGF 156

Query: 124 ----------------------------------------DSPNTDGIHISSSNGINIMH 183
                                                   DSPNTDGI + S + ++I +
Sbjct: 157 AFVNNSNIKDITSLNSKMGHFNFFSVHHFNITGVTITASGDSPNTDGIKMGSCSNMHISN 216

Query: 184 SVIGTGDDCISLGPGSKFINISNVQCGPGHGISIGSLGKYPNKEDVFEVTVRDSTFVGTS 243
           + IGTGDDCI++  G+  ++ISNV+CGPGHGIS+GSLGK  +++DV  +TVRD  F GTS
Sbjct: 217 TNIGTGDDCIAILSGTTNLDISNVKCGPGHGISVGSLGKNKDEKDVKNLTVRDVIFNGTS 276

Query: 244 NGVRIKSWSSSYSS-MVSKIVYLNLQMNNVKNPIIIDQDKMWF---YGWQMSKSRVEIKD 303
           +G+RIK+W SS S  +VS  VY N+QM +V  PI IDQ           Q  +S V+I+D
Sbjct: 277 DGIRIKTWESSASKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHEQKGESHVQIQD 336

Query: 304 VRYEGIRGSSNSQVAVNFECSQVFPCQGIVLQDINLPFNG-GGQPTTSICHNVRGSTFGQ 319
           ++ + I G+S ++VA+N +CS+ FPC+ I L DIN+  NG     + ++C NV GS  G+
Sbjct: 337 LKLKNIYGTSKNKVAMNLQCSKSFPCKNIELIDINIKSNGLENSSSIAVCENVDGSMSGK 396

The following BLAST results are available for this feature:

BLAST of Lsi05G005320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P35339	8.1e-65	37.85	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
Q6H9K0	1.1e-64	36.91	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P26216	3.1e-64	38.40	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]
P24548	8.9e-64	38.03	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
P35338	1.2e-63	38.12	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1	[more]

BLAST of Lsi05G005320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7H9	6.3e-145	73.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1	[more]
A0A5D3B9J1	8.8e-139	71.11	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S4DX91	1.1e-138	70.83	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103491147 PE=3 SV=1	[more]
A0A5D3BB58	1.6e-132	70.94	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1F153	3.3e-117	60.38	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=...	[more]

BLAST of Lsi05G005320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904214.1	1.1e-146	73.46	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_031738767.1	1.3e-144	73.13	exopolygalacturonase [Cucumis sativus]	[more]
KAA0065793.1	1.8e-138	71.11	exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96532.1 exopolygalactur...	[more]
XP_038904218.1	2.4e-138	73.49	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_016900599.1	2.4e-138	70.83	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]

BLAST of Lsi05G005320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G43080.1	8.9e-67	38.21	Pectin lyase-like superfamily protein	[more]
AT2G15450.1	3.4e-66	38.96	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	1.7e-65	38.36	Pectin lyase-like superfamily protein	[more]
AT1G43090.1	2.2e-65	38.67	Pectin lyase-like superfamily protein	[more]
AT2G40310.1	2.2e-65	38.15	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 112..316 e-value: 9.3E-60 score: 204.7 coord: 1..111 e-value: 3.9E-19 score: 70.9
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 112..305 e-value: 7.8E-60 score: 202.6 coord: 25..108 e-value: 8.0E-6 score: 25.1
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 159..172
None	No IPR available	PANTHER	PTHR31375:SF124	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 110..304
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 110..304
None	No IPR available	PANTHER	PTHR31375:SF124	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 2..108
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..108
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..318

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G005320.1	Lsi05G005320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity