Lsi05G005230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G005230
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	WRKY family transcription factor family protein
Location	chr05: 6549831 .. 6550794 (-)
RNA-Seq Expression	Lsi05G005230
Synteny	Lsi05G005230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTATTAGTACTAGCTCAATTCTTTTGCTTTTGTGTCTAAATTTTAGCCACAATCATACTGTAATTCAGTTTCCCTTTTTTTTTTCCGAGAAGGTGCATGTAATGTTGAATTTTTTTTTGATATATTAGTACTTAGCTCAATTCTTTTACTTTTGTATCTAAATTTTAGCTACAATCATACTGTACAAATCAGTTTCCCTTTTTTATTTTCTCAAGAAGGCACGCGATTGGGATTAGTAGCCATCCTTATCTGTTGTGCCCTTCCAACTTGAAGTTATGTGCCCTGAATCTCTGATATCAATATTTACCTATTTTAATGTTTTTTTTTTAATTAATTTATTTATTTATTTTTGAAAAAGGAGACGTTTTTTTCTCAAGAAAGCCTTTCATGTTTATGGGATGCTACCATTGTTATATTAATAGCACACTCATACTTGTATGCCTTATTCACTATTCCAATGATGCAGGAGTTATTACAAATGCACAAATGTTGGATGCCCCGTTAGAAAACATGTCGAAAGAGCATCCCATGACCCAAAGGCCGTTATAACTACGTATGAGGGGAAACATAATCACGATGTTCCCACTGCAAAAACTAGTAGCCATGATGTCGGAGGCCCGTCGACAATTGCACCATCGAGGTATAGACTCGAGGAGAGTGACACCATAAGCCTTGATCTTGGCGTTGGCATTGGCACGGGTGGTGAGAATAGATCAAATGAATACAGGCAGGCATTGCATTCACAACTTGTTGAAAACCAAACTCCAAATGGCAATTTCAACTTTGAAGTAGTTCAAGAGAACTCGGCTCCGACCTATTTTGGTGTGATCAATCGAGGTATAAATCAACATGGATCGAGAGAGAGTTTGAGCGAAAGCCGCAATAGGGAGATTGCTCCACTAAATCATTCATCTCATCCATATCCGACGAGCATTGGGAGAATACTAACAGGGCCTTGA

mRNA sequence

ATGATCCACAATCATACTGTAATTCAGTTTCCCTTTTTTTTTTCCGAGAAGGTGCATCTACAATCATACTGTACAAATCAGTTTCCCTTTTTTATTTTCTCAAGAAGGCACGCGATTGGGATTAGTAGCCATCCTTATCTGTTGTGCCCTTCCAACTTGAAGAGACGTTTTTTTCTCAAGAAAGCCTTTCATGTTTATGGGATGCTACCATTGAGTTATTACAAATGCACAAATGTTGGATGCCCCGTTAGAAAACATGTCGAAAGAGCATCCCATGACCCAAAGGCCGTTATAACTACGTATGAGGGGAAACATAATCACGATGTTCCCACTGCAAAAACTAGTAGCCATGATGTCGGAGGCCCGTCGACAATTGCACCATCGAGGTATAGACTCGAGGAGAGTGACACCATAAGCCTTGATCTTGGCGTTGGCATTGGCACGGGTGGTGAGAATAGATCAAATGAATACAGGCAGGCATTGCATTCACAACTTGTTGAAAACCAAACTCCAAATGGCAATTTCAACTTTGAAGTAGTTCAAGAGAACTCGGCTCCGACCTATTTTGGTGTGATCAATCGAGGTATAAATCAACATGGATCGAGAGAGAGTTTGAGCGAAAGCCGCAATAGGGAGATTGCTCCACTAAATCATTCATCTCATCCATATCCGACGAGCATTGGGAGAATACTAACAGGGCCTTGA

Coding sequence (CDS)

ATGATCCACAATCATACTGTAATTCAGTTTCCCTTTTTTTTTTCCGAGAAGGTGCATCTACAATCATACTGTACAAATCAGTTTCCCTTTTTTATTTTCTCAAGAAGGCACGCGATTGGGATTAGTAGCCATCCTTATCTGTTGTGCCCTTCCAACTTGAAGAGACGTTTTTTTCTCAAGAAAGCCTTTCATGTTTATGGGATGCTACCATTGAGTTATTACAAATGCACAAATGTTGGATGCCCCGTTAGAAAACATGTCGAAAGAGCATCCCATGACCCAAAGGCCGTTATAACTACGTATGAGGGGAAACATAATCACGATGTTCCCACTGCAAAAACTAGTAGCCATGATGTCGGAGGCCCGTCGACAATTGCACCATCGAGGTATAGACTCGAGGAGAGTGACACCATAAGCCTTGATCTTGGCGTTGGCATTGGCACGGGTGGTGAGAATAGATCAAATGAATACAGGCAGGCATTGCATTCACAACTTGTTGAAAACCAAACTCCAAATGGCAATTTCAACTTTGAAGTAGTTCAAGAGAACTCGGCTCCGACCTATTTTGGTGTGATCAATCGAGGTATAAATCAACATGGATCGAGAGAGAGTTTGAGCGAAAGCCGCAATAGGGAGATTGCTCCACTAAATCATTCATCTCATCCATATCCGACGAGCATTGGGAGAATACTAACAGGGCCTTGA

Protein sequence

MIHNHTVIQFPFFFSEKVHLQSYCTNQFPFFIFSRRHAIGISSHPYLLCPSNLKRRFFLKKAFHVYGMLPLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSRYRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYFGVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP

Homology

[Close]

BLAST of Lsi05G005230 vs. ExPASy Swiss-Prot
Match: Q93WV0 (Probable WRKY transcription factor 20 OS=Arabidopsis thaliana OX=3702 GN=WRKY20 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-43
Identity = 89/165 (53.94%), Postives = 119/165 (72.12%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT  GCPVRKHVERASHDPKAVITTYEGKH+HDVPT+K+SS+    P      R
Sbjct: 400 PRSYYKCTAHGCPVRKHVERASHDPKAVITTYEGKHDHDVPTSKSSSNHEIQP------R 459

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           +R +E+DTISL+LGVGI + G N ++   Q  + QLV    PNG  NF  V  +   +Y+
Sbjct: 460 FRPDETDTISLNLGVGISSDGPNHASNEHQHQNQQLVNQTHPNG-VNFRFVHASPMSSYY 519

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
             +N G+NQ+G RE+ +E++N +I+ LN+SS+PYP ++GR+ +GP
Sbjct: 520 ASLNSGMNQYGQRETKNETQNGDISSLNNSSYPYPPNMGRVQSGP 557

[Close]

BLAST of Lsi05G005230 vs. ExPASy Swiss-Prot
Match: Q6VWJ6 (WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 72/194 (37.11%), Postives = 102/194 (52.58%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGP------- 129
           P SYYKCT+ GCPVRKHVERASHDPK+VITTYEGKHNH+VP A+ ++H++  P       
Sbjct: 382 PRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHNHEVPAARNATHEMSAPPMKNVVH 441

Query: 130 --STIAPSRY-----------------RLEESDTISLDLGVGIGTGGENRSNEYRQALHS 189
             ++  PS                   +  E+DT+SLDLGVGI     + +N+ + +   
Sbjct: 442 QINSNMPSSIGGMMRACEARNYTNQYSQAAETDTVSLDLGVGISPNHSDATNQMQSSGPD 501

Query: 190 QLVENQTPNGNF--NFEVVQENSAPTYFGVINRGINQHGSRESL-SESRNREIAPLNHSS 235
           Q+       G+   N       +AP   G  N     +GSRE   +E       P++HS+
Sbjct: 502 QMQYQMQTMGSMYGNMRHPSSMAAPAVQG--NSAARMYGSREEKGNEGFTFRATPMDHSA 561

[Close]

BLAST of Lsi05G005230 vs. ExPASy Swiss-Prot
Match: Q9FG77 (Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.9e-19
Identity = 55/117 (47.01%), Postives = 62/117 (52.99%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT  GC VRKHVERASHD K+VITTYEGKHNHDVP A+ SSH  GG        
Sbjct: 506 PRSYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHNHDVPAARNSSHGGGG-------- 565

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAP 187
                      D G G   G    S+ Y    HS     + P G F+ +V   N +P
Sbjct: 566 -----------DSGNGNSGGSAAVSHHYHNGHHS-----EPPRGRFDRQVTTNNQSP 598

[Close]

BLAST of Lsi05G005230 vs. ExPASy Swiss-Prot
Match: Q8S8P5 (Probable WRKY transcription factor 33 OS=Arabidopsis thaliana OX=3702 GN=WRKY33 PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.3e-16
Identity = 51/115 (44.35%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT +GCPVRKHVERASHD +AVITTYEGKHNHDVP A+ S    G  +  AP  
Sbjct: 381 PRSYYKCTTIGCPVRKHVERASHDMRAVITTYEGKHNHDVPAARGS----GYATNRAP-- 440

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENS 185
              ++S ++ +      G      S++    L   L  N T  G F + +   N+
Sbjct: 441 ---QDSSSVPIRPAAIAGHSNYTTSSQAPYTL-QMLHNNNTNTGPFGYAMNNNNN 485

[Close]

BLAST of Lsi05G005230 vs. ExPASy Swiss-Prot
Match: Q9XI90 (Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE=1 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 3.0e-16
Identity = 38/48 (79.17%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH 118
           P SYYKCT  GC VRKHVERA+ DPKAV+TTYEGKHNHD+P AK+SSH
Sbjct: 428 PRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSH 475

[Close]

BLAST of Lsi05G005230 vs. ExPASy TrEMBL
Match: A0A6J1DL61 (probable WRKY transcription factor 20 OS=Momordica charantia OX=3673 GN=LOC111022132 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.3e-80
Identity = 149/167 (89.22%), Postives = 155/167 (92.81%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPT KTSSHD+ GP TIAPSR
Sbjct: 351 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTTKTSSHDIAGPPTIAPSR 410

Query: 130 YRLEESDTISLDLGVGIGT--GGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPT 189
           YRLEESDTISLDLGVG+GT  GGENR NEYRQALHSQLVENQTPNGNFNFE  QEN APT
Sbjct: 411 YRLEESDTISLDLGVGLGTGMGGENRPNEYRQALHSQLVENQTPNGNFNFEEAQENPAPT 470

Query: 190 YFGVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           YFGV+NRGINQHGSRESLSES + EIAPLNHSSHPYP++IGRILTGP
Sbjct: 471 YFGVLNRGINQHGSRESLSESHSIEIAPLNHSSHPYPSTIGRILTGP 517

[Close]

BLAST of Lsi05G005230 vs. ExPASy TrEMBL
Match: A0A6J1KYX1 (probable WRKY transcription factor 20 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498371 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.9e-75
Identity = 143/165 (86.67%), Postives = 148/165 (89.70%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH+V G  TIAPSR
Sbjct: 417 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHEVQGSLTIAPSR 476

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQA HSQL ENQ PNGNFNFE+VQENS PTYF
Sbjct: 477 YRLEESDTISLDLGVGIGAGGENRSNEYRQAFHSQLAENQNPNGNFNFEIVQENSPPTYF 536

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GV++RGI+QHGSRESLSESR      LNHSSHPYP SI RILTGP
Sbjct: 537 GVLDRGISQHGSRESLSESRTMS---LNHSSHPYPPSIERILTGP 578

[Close]

BLAST of Lsi05G005230 vs. ExPASy TrEMBL
Match: A0A6J1KX63 (probable WRKY transcription factor 20 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498371 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.9e-75
Identity = 143/165 (86.67%), Postives = 148/165 (89.70%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH+V G  TIAPSR
Sbjct: 333 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHEVQGSLTIAPSR 392

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQA HSQL ENQ PNGNFNFE+VQENS PTYF
Sbjct: 393 YRLEESDTISLDLGVGIGAGGENRSNEYRQAFHSQLAENQNPNGNFNFEIVQENSPPTYF 452

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GV++RGI+QHGSRESLSESR      LNHSSHPYP SI RILTGP
Sbjct: 453 GVLDRGISQHGSRESLSESRTMS---LNHSSHPYPPSIERILTGP 494

[Close]

BLAST of Lsi05G005230 vs. ExPASy TrEMBL
Match: A0A6J1EYS4 (probable WRKY transcription factor 20 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439705 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.9e-75
Identity = 143/165 (86.67%), Postives = 148/165 (89.70%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH+V G  TIAPSR
Sbjct: 333 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHEVQGSLTIAPSR 392

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQA HSQL ENQ PNGNFNFE+VQENS PTYF
Sbjct: 393 YRLEESDTISLDLGVGIGAGGENRSNEYRQAFHSQLAENQNPNGNFNFEIVQENSPPTYF 452

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GV++RGI+QHGSRESLSESR      LNHSSHPYP SI RILTGP
Sbjct: 453 GVLDRGISQHGSRESLSESRTMS---LNHSSHPYPPSIERILTGP 494

[Close]

BLAST of Lsi05G005230 vs. ExPASy TrEMBL
Match: A0A6J1EYM7 (probable WRKY transcription factor 20 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439705 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.9e-75
Identity = 143/165 (86.67%), Postives = 148/165 (89.70%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH+V G  TIAPSR
Sbjct: 417 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHEVQGSLTIAPSR 476

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQA HSQL ENQ PNGNFNFE+VQENS PTYF
Sbjct: 477 YRLEESDTISLDLGVGIGAGGENRSNEYRQAFHSQLAENQNPNGNFNFEIVQENSPPTYF 536

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GV++RGI+QHGSRESLSESR      LNHSSHPYP SI RILTGP
Sbjct: 537 GVLDRGISQHGSRESLSESRTMS---LNHSSHPYPPSIERILTGP 578

[Close]

BLAST of Lsi05G005230 vs. NCBI nr
Match: XP_038904071.1 (probable WRKY transcription factor 20 isoform X1 [Benincasa hispida])

HSP 1 Score: 327.8 bits (839), Expect = 7.7e-86
Identity = 159/165 (96.36%), Postives = 159/165 (96.36%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAP R
Sbjct: 415 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPLR 474

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENS PTYF
Sbjct: 475 YRLEESDTISLDLGVGIGMGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSGPTYF 534

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GVINRGINQHGSRESLSESRNREI PLNHSSHPY TSIGRILTGP
Sbjct: 535 GVINRGINQHGSRESLSESRNREITPLNHSSHPYSTSIGRILTGP 579

[Close]

BLAST of Lsi05G005230 vs. NCBI nr
Match: XP_038904072.1 (probable WRKY transcription factor 20 isoform X2 [Benincasa hispida] >XP_038904074.1 probable WRKY transcription factor 20 isoform X2 [Benincasa hispida])

HSP 1 Score: 327.8 bits (839), Expect = 7.7e-86
Identity = 159/165 (96.36%), Postives = 159/165 (96.36%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAP R
Sbjct: 335 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPLR 394

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENS PTYF
Sbjct: 395 YRLEESDTISLDLGVGIGMGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSGPTYF 454

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GVINRGINQHGSRESLSESRNREI PLNHSSHPY TSIGRILTGP
Sbjct: 455 GVINRGINQHGSRESLSESRNREITPLNHSSHPYSTSIGRILTGP 499

[Close]

BLAST of Lsi05G005230 vs. NCBI nr
Match: XP_022154985.1 (probable WRKY transcription factor 20 [Momordica charantia])

HSP 1 Score: 308.5 bits (789), Expect = 4.8e-80
Identity = 149/167 (89.22%), Postives = 155/167 (92.81%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPT KTSSHD+ GP TIAPSR
Sbjct: 351 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTTKTSSHDIAGPPTIAPSR 410

Query: 130 YRLEESDTISLDLGVGIGT--GGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPT 189
           YRLEESDTISLDLGVG+GT  GGENR NEYRQALHSQLVENQTPNGNFNFE  QEN APT
Sbjct: 411 YRLEESDTISLDLGVGLGTGMGGENRPNEYRQALHSQLVENQTPNGNFNFEEAQENPAPT 470

Query: 190 YFGVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           YFGV+NRGINQHGSRESLSES + EIAPLNHSSHPYP++IGRILTGP
Sbjct: 471 YFGVLNRGINQHGSRESLSESHSIEIAPLNHSSHPYPSTIGRILTGP 517

[Close]

BLAST of Lsi05G005230 vs. NCBI nr
Match: KAG6596743.1 (putative WRKY transcription factor 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.1 bits (749), Expect = 2.1e-75
Identity = 144/165 (87.27%), Postives = 149/165 (90.30%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH+V G  TIAPSR
Sbjct: 417 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHEVQGSLTIAPSR 476

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIGTGGENRSNEYRQA HSQL ENQ PNGNFNFE+VQENS PTYF
Sbjct: 477 YRLEESDTISLDLGVGIGTGGENRSNEYRQAFHSQLAENQNPNGNFNFEIVQENSPPTYF 536

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GV++RGI+QHGSRESLSESR      LNHSSHPYP SI RILTGP
Sbjct: 537 GVLDRGISQHGSRESLSESRTMS---LNHSSHPYPPSIERILTGP 578

[Close]

BLAST of Lsi05G005230 vs. NCBI nr
Match: XP_023005350.1 (probable WRKY transcription factor 20 isoform X2 [Cucurbita maxima])

HSP 1 Score: 291.2 bits (744), Expect = 8.0e-75
Identity = 143/165 (86.67%), Postives = 148/165 (89.70%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH+V G  TIAPSR
Sbjct: 333 PRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHEVQGSLTIAPSR 392

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           YRLEESDTISLDLGVGIG GGENRSNEYRQA HSQL ENQ PNGNFNFE+VQENS PTYF
Sbjct: 393 YRLEESDTISLDLGVGIGAGGENRSNEYRQAFHSQLAENQNPNGNFNFEIVQENSPPTYF 452

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
           GV++RGI+QHGSRESLSESR      LNHSSHPYP SI RILTGP
Sbjct: 453 GVLDRGISQHGSRESLSESRTMS---LNHSSHPYPPSIERILTGP 494

[Close]

BLAST of Lsi05G005230 vs. TAIR 10
Match: AT4G26640.1 (WRKY family transcription factor family protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-44
Identity = 89/165 (53.94%), Postives = 119/165 (72.12%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT  GCPVRKHVERASHDPKAVITTYEGKH+HDVPT+K+SS+    P      R
Sbjct: 328 PRSYYKCTAHGCPVRKHVERASHDPKAVITTYEGKHDHDVPTSKSSSNHEIQP------R 387

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           +R +E+DTISL+LGVGI + G N ++   Q  + QLV    PNG  NF  V  +   +Y+
Sbjct: 388 FRPDETDTISLNLGVGISSDGPNHASNEHQHQNQQLVNQTHPNG-VNFRFVHASPMSSYY 447

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
             +N G+NQ+G RE+ +E++N +I+ LN+SS+PYP ++GR+ +GP
Sbjct: 448 ASLNSGMNQYGQRETKNETQNGDISSLNNSSYPYPPNMGRVQSGP 485

[Close]

BLAST of Lsi05G005230 vs. TAIR 10
Match: AT4G26640.2 (WRKY family transcription factor family protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-44
Identity = 89/165 (53.94%), Postives = 119/165 (72.12%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT  GCPVRKHVERASHDPKAVITTYEGKH+HDVPT+K+SS+    P      R
Sbjct: 400 PRSYYKCTAHGCPVRKHVERASHDPKAVITTYEGKHDHDVPTSKSSSNHEIQP------R 459

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAPTYF 189
           +R +E+DTISL+LGVGI + G N ++   Q  + QLV    PNG  NF  V  +   +Y+
Sbjct: 460 FRPDETDTISLNLGVGISSDGPNHASNEHQHQNQQLVNQTHPNG-VNFRFVHASPMSSYY 519

Query: 190 GVINRGINQHGSRESLSESRNREIAPLNHSSHPYPTSIGRILTGP 235
             +N G+NQ+G RE+ +E++N +I+ LN+SS+PYP ++GR+ +GP
Sbjct: 520 ASLNSGMNQYGQRETKNETQNGDISSLNNSSYPYPPNMGRVQSGP 557

[Close]

BLAST of Lsi05G005230 vs. TAIR 10
Match: AT5G56270.1 (WRKY DNA-binding protein 2 )

HSP 1 Score: 96.3 bits (238), Expect = 3.5e-20
Identity = 55/117 (47.01%), Postives = 62/117 (52.99%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT  GC VRKHVERASHD K+VITTYEGKHNHDVP A+ SSH  GG        
Sbjct: 506 PRSYYKCTAPGCTVRKHVERASHDLKSVITTYEGKHNHDVPAARNSSHGGGG-------- 565

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENSAP 187
                      D G G   G    S+ Y    HS     + P G F+ +V   N +P
Sbjct: 566 -----------DSGNGNSGGSAAVSHHYHNGHHS-----EPPRGRFDRQVTTNNQSP 598

[Close]

BLAST of Lsi05G005230 vs. TAIR 10
Match: AT2G38470.1 (WRKY DNA-binding protein 33 )

HSP 1 Score: 88.2 bits (217), Expect = 9.6e-18
Identity = 51/115 (44.35%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSHDVGGPSTIAPSR 129
           P SYYKCT +GCPVRKHVERASHD +AVITTYEGKHNHDVP A+ S    G  +  AP  
Sbjct: 381 PRSYYKCTTIGCPVRKHVERASHDMRAVITTYEGKHNHDVPAARGS----GYATNRAP-- 440

Query: 130 YRLEESDTISLDLGVGIGTGGENRSNEYRQALHSQLVENQTPNGNFNFEVVQENS 185
              ++S ++ +      G      S++    L   L  N T  G F + +   N+
Sbjct: 441 ---QDSSSVPIRPAAIAGHSNYTTSSQAPYTL-QMLHNNNTNTGPFGYAMNNNNN 485

[Close]

BLAST of Lsi05G005230 vs. TAIR 10
Match: AT1G13960.1 (WRKY DNA-binding protein 4 )

HSP 1 Score: 87.0 bits (214), Expect = 2.1e-17
Identity = 38/48 (79.17%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 70  PLSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHDVPTAKTSSH 118
           P SYYKCT  GC VRKHVERA+ DPKAV+TTYEGKHNHD+P AK+SSH
Sbjct: 428 PRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSH 475

The following BLAST results are available for this feature:

BLAST of Lsi05G005230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WV0	1.7e-43	53.94	Probable WRKY transcription factor 20 OS=Arabidopsis thaliana OX=3702 GN=WRKY20 ...	[more]
Q6VWJ6	2.7e-25	37.11	WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1	[more]
Q9FG77	4.9e-19	47.01	Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE...	[more]
Q8S8P5	1.3e-16	44.35	Probable WRKY transcription factor 33 OS=Arabidopsis thaliana OX=3702 GN=WRKY33 ...	[more]
Q9XI90	3.0e-16	79.17	Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE...	[more]

BLAST of Lsi05G005230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DL61	2.3e-80	89.22	probable WRKY transcription factor 20 OS=Momordica charantia OX=3673 GN=LOC11102...	[more]
A0A6J1KYX1	3.9e-75	86.67	probable WRKY transcription factor 20 isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1KX63	3.9e-75	86.67	probable WRKY transcription factor 20 isoform X2 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1EYS4	3.9e-75	86.67	probable WRKY transcription factor 20 isoform X2 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1EYM7	3.9e-75	86.67	probable WRKY transcription factor 20 isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of Lsi05G005230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904071.1	7.7e-86	96.36	probable WRKY transcription factor 20 isoform X1 [Benincasa hispida]	[more]
XP_038904072.1	7.7e-86	96.36	probable WRKY transcription factor 20 isoform X2 [Benincasa hispida] >XP_0389040...	[more]
XP_022154985.1	4.8e-80	89.22	probable WRKY transcription factor 20 [Momordica charantia]	[more]
KAG6596743.1	2.1e-75	87.27	putative WRKY transcription factor 20, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023005350.1	8.0e-75	86.67	probable WRKY transcription factor 20 isoform X2 [Cucurbita maxima]	[more]

BLAST of Lsi05G005230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26640.1	1.2e-44	53.94	WRKY family transcription factor family protein	[more]
AT4G26640.2	1.2e-44	53.94	WRKY family transcription factor family protein	[more]
AT5G56270.1	3.5e-20	47.01	WRKY DNA-binding protein 2	[more]
AT2G38470.1	9.6e-18	44.35	WRKY DNA-binding protein 33	[more]
AT1G13960.1	2.1e-17	79.17	WRKY DNA-binding protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 66..109 e-value: 2.2E-11 score: 53.8
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 70..108 e-value: 1.2E-12 score: 47.8
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 70..110 score: 22.779257
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 59..111 e-value: 7.2E-17 score: 62.9
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 68..110
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 200..234
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 106..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 210..226
None	No IPR available	PANTHER	PTHR31221:SF175	WRKY TRANSCRIPTION FACTOR 20 ISOFORM X1-RELATED	coord: 70..209
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 70..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G005230.1	Lsi05G005230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding