Lsi05G004970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G004970
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein TIC 20
Locationchr05: 6269061 .. 6269693 (+)
RNA-Seq ExpressionLsi05G004970
SyntenyLsi05G004970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTTTCCTCGCCATTTCCCTCTCTCTCAAACCCTACCCAAATCAACTTCACCGCCACTCTGCGACGGCGCCGACAACGCATCCTCACCGTCCGATCCACCGGCGACAACAACAACAACTCCGCCGACGGCCCTGACCGCCTCATCTCCGCCATCTGCTACTTCTACCCCTTCTTCGATGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTATGATTCAACCCTTGGTTCCGGCCATTCGAGTTTTCAAGAGCTTTCCATTGAATAGCTTTCTGGTGTTTTTGACTCTTTACTTCGTCGTGGTTAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATAATGCAAGCCATTGTTCTTGACGTGCTTCTGATTTTTCCTGATCTTTTGGAAAGGAGCTTCAATCCTAGAGGGGGATTAGGGTTGGATTTGGTCATGAGTTTGGATAGTACTGTGTTCCTGTTTCTTTTGATTTGTTTGATTTATGGCTCTTCTTCTTGCTTGTTGGGTCAGATCCCTAGATTGCCCATTGTTGCTGATGCTGCTGATAGGCAAGTTATGTGA

mRNA sequence

ATGGCGATGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTTTCCTCGCCATTTCCCTCTCTCTCAAACCCTACCCAAATCAACTTCACCGCCACTCTGCGACGGCGCCGACAACGCATCCTCACCGTCCGATCCACCGGCGACAACAACAACAACTCCGCCGACGGCCCTGACCGCCTCATCTCCGCCATCTGCTACTTCTACCCCTTCTTCGATGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTATGATTCAACCCTTGGTTCCGGCCATTCGAGTTTTCAAGAGCTTTCCATTGAATAGCTTTCTGGTGTTTTTGACTCTTTACTTCGTCGTGGTTAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATAATGCAAGCCATTGTTCTTGACGTGCTTCTGATTTTTCCTGATCTTTTGGAAAGGAGCTTCAATCCTAGAGGGGGATTAGGGTTGGATTTGGTCATGAGTTTGGATAGTACTGTGTTCCTGTTTCTTTTGATTTGTTTGATTTATGGCTCTTCTTCTTGCTTGTTGGGTCAGATCCCTAGATTGCCCATTGTTGCTGATGCTGCTGATAGGCAAGTTATGTGA

Coding sequence (CDS)

ATGGCGATGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTTTCCTCGCCATTTCCCTCTCTCTCAAACCCTACCCAAATCAACTTCACCGCCACTCTGCGACGGCGCCGACAACGCATCCTCACCGTCCGATCCACCGGCGACAACAACAACAACTCCGCCGACGGCCCTGACCGCCTCATCTCCGCCATCTGCTACTTCTACCCCTTCTTCGATGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTATGATTCAACCCTTGGTTCCGGCCATTCGAGTTTTCAAGAGCTTTCCATTGAATAGCTTTCTGGTGTTTTTGACTCTTTACTTCGTCGTGGTTAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATAATGCAAGCCATTGTTCTTGACGTGCTTCTGATTTTTCCTGATCTTTTGGAAAGGAGCTTCAATCCTAGAGGGGGATTAGGGTTGGATTTGGTCATGAGTTTGGATAGTACTGTGTTCCTGTTTCTTTTGATTTGTTTGATTTATGGCTCTTCTTCTTGCTTGTTGGGTCAGATCCCTAGATTGCCCATTGTTGCTGATGCTGCTGATAGGCAAGTTATGTGA

Protein sequence

MAMLILNLSPKTLHSSFFSSPFPSLSNPTQINFTATLRRRRQRILTVRSTGDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM
Homology
[Close]
BLAST of Lsi05G004970 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 8.0e-69
Identity = 130/174 (74.71%), Postives = 148/174 (85.06%), Query Frame = 0 

Query: 37  LRRRRQRILTVRSTGDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQ 96
           L R R   L ++S GD   +S D  DR+ISA+CYFYPFFDG+QYGK++ITQY PFQ++IQ
Sbjct: 39  LSRDRAATLVLQSKGD---DSVDASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQ 98

Query: 97  PLVPAIRVFKSFPLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERS 156
           PL PAIR FKSFP N FL+F+TLYFVVVRNPNFSRYVRFN MQAIVLDVLLIFPDLLERS
Sbjct: 99  PLFPAIRAFKSFPFNGFLIFITLYFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERS 158

Query: 157 FNPRGGLGLDLVMSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           FNPR G GLD+VMSLDSTVFLFLL+ LIYG S+CL G  PRLP+VA+AADRQV+
Sbjct: 159 FNPRDGFGLDVVMSLDSTVFLFLLVSLIYGFSACLFGLTPRLPLVAEAADRQVL 209

[Close]
BLAST of Lsi05G004970 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.9e-31
Identity = 79/188 (42.02%), Postives = 110/188 (58.51%), Query Frame = 0 

Query: 24  SLSNPTQINFTATLRRRRQRILTVRSTGDNNNNSADGPDRLISAICYFYPFFDGVQYGKY 83
           SLS P    F+     RR   LT R+    +       +R+IS   Y  PFF+ +QYG++
Sbjct: 25  SLSFPGSSTFSIRPSSRRATALTTRA----SYTPTPATERVISIASYALPFFNSLQYGRF 84

Query: 84  VITQYAPFQVMIQPLVPAIRVFKSFPLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVL 143
           +  QY    ++ +P+ P + +++S P  SF+ F  LY  VVRN +FSRYVRFN MQA+ L
Sbjct: 85  LFAQYPRLGLLFEPIFPILNLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTL 144

Query: 144 DVLLIFPDLLERSFNP--RGGLGLDLVMSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIV 203
           DVLL  P LL R  +P   GG G+  +M   + VF+F  +C +YG  S LLG+ P +P V
Sbjct: 145 DVLLAVPVLLTRILDPGQGGGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFV 204

Query: 204 ADAADRQV 210
           ADAA RQ+
Sbjct: 205 ADAAGRQL 208

[Close]
BLAST of Lsi05G004970 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-21
Identity = 56/157 (35.67%), Postives = 93/157 (59.24%), Query Frame = 0 

Query: 54  NNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSF 113
           +N++ADG DR  SA+ Y  P  D   +G +++ Q+   Q++  P++P ++ +  FP  SF
Sbjct: 3   SNSTADGKDRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASF 62

Query: 114 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLL-IFPDLLERSFNPRGGLGLDLVMSLD 173
           ++F+ L+  VVRN N S ++RFN MQAI++ +LL +F  ++     P  G GL +  +L 
Sbjct: 63  IIFIVLFMAVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGL-VTETLY 122

Query: 174 STVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQV 210
           +  FL  L C  +G    +LG+   +P ++DAA  QV
Sbjct: 123 NFAFLGALACGFFGIVQSVLGRYAEIPTISDAAYSQV 158

[Close]
BLAST of Lsi05G004970 vs. ExPASy TrEMBL
Match: A0A5D3CPD4 (Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00160 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.3e-102
Identity = 199/215 (92.56%), Postives = 204/215 (94.88%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP--TQINFTATLRRR---RQRILTVRSTGDNNN 60
           MA LILNLSPKTLHSSFF SPFP+LSNP  T +NFT TLRRR   R+RI TVRSTGDNNN
Sbjct: 1   MAFLILNLSPKTLHSSFFPSPFPALSNPKTTTLNFTTTLRRRGRGRRRIFTVRSTGDNNN 60

Query: 61  NSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSFLV 120
           NSADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQV+IQPLVPAIRVFKSFPLNSFLV
Sbjct: 61  NSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSFLV 120

Query: 121 FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTV 180
           FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG+GLDLVMSLDSTV
Sbjct: 121 FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLVMSLDSTV 180

Query: 181 FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM
Sbjct: 181 FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 215

[Close]
BLAST of Lsi05G004970 vs. ExPASy TrEMBL
Match: A0A1S3BM16 (Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103491027 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.3e-102
Identity = 199/215 (92.56%), Postives = 204/215 (94.88%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP--TQINFTATLRRR---RQRILTVRSTGDNNN 60
           MA LILNLSPKTLHSSFF SPFP+LSNP  T +NFT TLRRR   R+RI TVRSTGDNNN
Sbjct: 1   MAFLILNLSPKTLHSSFFPSPFPALSNPKTTTLNFTTTLRRRGRGRRRIFTVRSTGDNNN 60

Query: 61  NSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSFLV 120
           NSADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQV+IQPLVPAIRVFKSFPLNSFLV
Sbjct: 61  NSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSFLV 120

Query: 121 FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTV 180
           FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG+GLDLVMSLDSTV
Sbjct: 121 FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLVMSLDSTV 180

Query: 181 FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM
Sbjct: 181 FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 215

[Close]
BLAST of Lsi05G004970 vs. ExPASy TrEMBL
Match: A0A6J1EW92 (Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111438760 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 4.1e-100
Identity = 195/220 (88.64%), Postives = 201/220 (91.36%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP----------TQINFTATLRRRRQRILTVRST 60
           MA LILNLSPKTLHSSFF SPFPSLSNP           QINFT   RRRR+RILTVRS+
Sbjct: 23  MATLILNLSPKTLHSSFFPSPFPSLSNPRTTAATTTLTNQINFTTLRRRRRRRILTVRSS 82

Query: 61  GDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPL 120
           GD +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQV+IQPLVPAIRVFKSFP 
Sbjct: 83  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 142

Query: 121 NSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180
           N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS
Sbjct: 143 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 202

Query: 181 LDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           LDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 203 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 242

[Close]
BLAST of Lsi05G004970 vs. ExPASy TrEMBL
Match: A0A6J1HN42 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111465112 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.6e-99
Identity = 194/219 (88.58%), Postives = 200/219 (91.32%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP---------TQINFTATLRRRRQRILTVRSTG 60
           MA LILNLSPKTL SSFF SPFPSLSNP          QINFT   RRRR+RILTVRS+G
Sbjct: 1   MATLILNLSPKTLRSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 60

Query: 61  DNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLN 120
           D +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQV+IQPLVPAIRVFKSFP N
Sbjct: 61  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 120

Query: 121 SFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180
            FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL
Sbjct: 121 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180

Query: 181 DSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           DSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 181 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 219

[Close]
BLAST of Lsi05G004970 vs. ExPASy TrEMBL
Match: A0A0A0L632 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_3G047830 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.0e-95
Identity = 195/216 (90.28%), Postives = 197/216 (91.20%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP--TQINFTATLRRR---RQRILTVRSTG-DNN 60
           MA LILNLSPKT HS F    FPSLSNP  T +NFT TL RR   R RILTVRSTG DNN
Sbjct: 1   MAFLILNLSPKTPHSPF----FPSLSNPKTTTLNFTTTLPRRWLARPRILTVRSTGDDNN 60

Query: 61  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSFL 120
           NNSAD PDRLISAICYFYPFFDGVQYGKYVITQY PFQV+IQPLVPAIRVFKSFPLNSFL
Sbjct: 61  NNSADAPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSFL 120

Query: 121 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDST 180
           VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDST
Sbjct: 121 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDST 180

Query: 181 VFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           VFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM
Sbjct: 181 VFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 212

[Close]
BLAST of Lsi05G004970 vs. NCBI nr
Match: XP_038905184.1 (protein TIC 20-v, chloroplastic [Benincasa hispida])

HSP 1 Score: 393.3 bits (1009), Expect = 1.3e-105
Identity = 205/211 (97.16%), Postives = 208/211 (98.58%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNPTQINFTATLRRRRQRILTVRSTGDNNN-NSAD 60
           MAMLILNLSPKTL+SSFF SPFPSLSNPTQINFTATL RRR+RILTVRSTGDNNN NSAD
Sbjct: 1   MAMLILNLSPKTLYSSFFPSPFPSLSNPTQINFTATLPRRRRRILTVRSTGDNNNSNSAD 60

Query: 61  GPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSFLVFLTL 120
           GPDRLISAICYFYPFFDGVQYGKYVITQYAPFQV+IQPLVPAIRVFKSFPLNSFLVFLTL
Sbjct: 61  GPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNSFLVFLTL 120

Query: 121 YFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFL 180
           YFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFL
Sbjct: 121 YFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFL 180

Query: 181 LICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           LICLIYGSSSCLLGQIPRLPIVADAADRQVM
Sbjct: 181 LICLIYGSSSCLLGQIPRLPIVADAADRQVM 211

[Close]
BLAST of Lsi05G004970 vs. NCBI nr
Match: XP_008449034.1 (PREDICTED: protein TIC 20-v, chloroplastic [Cucumis melo] >KAA0065731.1 protein TIC 20-v [Cucumis melo var. makuwa] >TYK13757.1 protein TIC 20-v [Cucumis melo var. makuwa])

HSP 1 Score: 380.9 bits (977), Expect = 6.9e-102
Identity = 199/215 (92.56%), Postives = 204/215 (94.88%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP--TQINFTATLRRR---RQRILTVRSTGDNNN 60
           MA LILNLSPKTLHSSFF SPFP+LSNP  T +NFT TLRRR   R+RI TVRSTGDNNN
Sbjct: 1   MAFLILNLSPKTLHSSFFPSPFPALSNPKTTTLNFTTTLRRRGRGRRRIFTVRSTGDNNN 60

Query: 61  NSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLNSFLV 120
           NSADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQV+IQPLVPAIRVFKSFPLNSFLV
Sbjct: 61  NSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSFLV 120

Query: 121 FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTV 180
           FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG+GLDLVMSLDSTV
Sbjct: 121 FLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLVMSLDSTV 180

Query: 181 FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM
Sbjct: 181 FLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 215

[Close]
BLAST of Lsi05G004970 vs. NCBI nr
Match: XP_023553383.1 (protein TIC 20-v, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.4 bits (960), Expect = 6.4e-100
Identity = 195/219 (89.04%), Postives = 201/219 (91.78%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP---------TQINFTATLRRRRQRILTVRSTG 60
           MA LILNLSPKTLHSSFF SPFPSLSNP          QINFT   RRRR+RILTVRS+G
Sbjct: 22  MATLILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSG 81

Query: 61  DNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPLN 120
           D +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQV+IQPLVPAIRVFKSFP N
Sbjct: 82  DRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFN 141

Query: 121 SFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 180
            FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL
Sbjct: 142 GFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSL 201

Query: 181 DSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           DSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 202 DSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 240

[Close]
BLAST of Lsi05G004970 vs. NCBI nr
Match: XP_022932396.1 (protein TIC 20-v, chloroplastic [Cucurbita moschata])

HSP 1 Score: 374.0 bits (959), Expect = 8.4e-100
Identity = 195/220 (88.64%), Postives = 201/220 (91.36%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP----------TQINFTATLRRRRQRILTVRST 60
           MA LILNLSPKTLHSSFF SPFPSLSNP           QINFT   RRRR+RILTVRS+
Sbjct: 23  MATLILNLSPKTLHSSFFPSPFPSLSNPRTTAATTTLTNQINFTTLRRRRRRRILTVRSS 82

Query: 61  GDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPL 120
           GD +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQV+IQPLVPAIRVFKSFP 
Sbjct: 83  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 142

Query: 121 NSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180
           N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS
Sbjct: 143 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 202

Query: 181 LDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           LDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 203 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 242

[Close]
BLAST of Lsi05G004970 vs. NCBI nr
Match: KAG6577521.1 (Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015580.1 Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.1 bits (954), Expect = 3.2e-99
Identity = 194/220 (88.18%), Postives = 200/220 (90.91%), Query Frame = 0 

Query: 1   MAMLILNLSPKTLHSSFFSSPFPSLSNP----------TQINFTATLRRRRQRILTVRST 60
           MA LILNLSPKTLHSSFF SPFPSL NP           QINFT   RRRR+RILTVRS+
Sbjct: 1   MAKLILNLSPKTLHSSFFPSPFPSLPNPRTTATTTTLTNQINFTTLRRRRRRRILTVRSS 60

Query: 61  GDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQPLVPAIRVFKSFPL 120
           GD +NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQV+IQPLVPAIRVFKSFP 
Sbjct: 61  GDRDNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPF 120

Query: 121 NSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180
           N FLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS
Sbjct: 121 NGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMS 180

Query: 181 LDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           LDSTVFLFLLICLIYGSSSCLLGQ+PRLPIVADAADRQVM
Sbjct: 181 LDSTVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQVM 220

[Close]
BLAST of Lsi05G004970 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 261.5 bits (667), Expect = 5.7e-70
Identity = 130/174 (74.71%), Postives = 148/174 (85.06%), Query Frame = 0 

Query: 37  LRRRRQRILTVRSTGDNNNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVMIQ 96
           L R R   L ++S GD   +S D  DR+ISA+CYFYPFFDG+QYGK++ITQY PFQ++IQ
Sbjct: 39  LSRDRAATLVLQSKGD---DSVDASDRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQ 98

Query: 97  PLVPAIRVFKSFPLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERS 156
           PL PAIR FKSFP N FL+F+TLYFVVVRNPNFSRYVRFN MQAIVLDVLLIFPDLLERS
Sbjct: 99  PLFPAIRAFKSFPFNGFLIFITLYFVVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERS 158

Query: 157 FNPRGGLGLDLVMSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQVM 211
           FNPR G GLD+VMSLDSTVFLFLL+ LIYG S+CL G  PRLP+VA+AADRQV+
Sbjct: 159 FNPRDGFGLDVVMSLDSTVFLFLLVSLIYGFSACLFGLTPRLPLVAEAADRQVL 209

[Close]
BLAST of Lsi05G004970 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 136.3 bits (342), Expect = 2.7e-32
Identity = 79/188 (42.02%), Postives = 110/188 (58.51%), Query Frame = 0 

Query: 24  SLSNPTQINFTATLRRRRQRILTVRSTGDNNNNSADGPDRLISAICYFYPFFDGVQYGKY 83
           SLS P    F+     RR   LT R+    +       +R+IS   Y  PFF+ +QYG++
Sbjct: 25  SLSFPGSSTFSIRPSSRRATALTTRA----SYTPTPATERVISIASYALPFFNSLQYGRF 84

Query: 84  VITQYAPFQVMIQPLVPAIRVFKSFPLNSFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVL 143
           +  QY    ++ +P+ P + +++S P  SF+ F  LY  VVRN +FSRYVRFN MQA+ L
Sbjct: 85  LFAQYPRLGLLFEPIFPILNLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAVTL 144

Query: 144 DVLLIFPDLLERSFNP--RGGLGLDLVMSLDSTVFLFLLICLIYGSSSCLLGQIPRLPIV 203
           DVLL  P LL R  +P   GG G+  +M   + VF+F  +C +YG  S LLG+ P +P V
Sbjct: 145 DVLLAVPVLLTRILDPGQGGGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIPFV 204

Query: 204 ADAADRQV 210
           ADAA RQ+
Sbjct: 205 ADAAGRQL 208

The following BLAST results are available for this feature:
BLAST of Lsi05G004970 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FM678.0e-6974.71Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 ... [more]
O822513.9e-3142.02Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=... [more]
P733872.1e-2135.67Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 ... [more]
BLAST of Lsi05G004970 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3CPD43.3e-10292.56Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00160... [more]
A0A1S3BM163.3e-10292.56Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103491027 PE=3 SV=1[more]
A0A6J1EW924.1e-10088.64Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111438760 PE=3 SV=1[more]
A0A6J1HN422.6e-9988.58Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111465112 PE=3 SV=1[more]
A0A0A0L6323.0e-9590.28Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_3G047830 PE=3 SV=1[more]
BLAST of Lsi05G004970 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038905184.11.3e-10597.16protein TIC 20-v, chloroplastic [Benincasa hispida][more]
XP_008449034.16.9e-10292.56PREDICTED: protein TIC 20-v, chloroplastic [Cucumis melo] >KAA0065731.1 protein ... [more]
XP_023553383.16.4e-10089.04protein TIC 20-v, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022932396.18.4e-10088.64protein TIC 20-v, chloroplastic [Cucurbita moschata][more]
KAG6577521.13.2e-9988.18Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]... [more]
BLAST of Lsi05G004970 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G55710.15.7e-7074.71FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT2G47840.12.7e-3242.02Uncharacterised conserved protein ycf60 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005691Chloroplast protein import component Tic20PFAMPF16166TIC20coord: 63..205
e-value: 3.6E-54
score: 183.3
IPR005691Chloroplast protein import component Tic20PANTHERPTHR33510PROTEIN TIC 20-II, CHLOROPLASTICcoord: 10..210
NoneNo IPR availablePANTHERPTHR33510:SF11PROTEIN TIC 20-V, CHLOROPLASTICcoord: 10..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G004970.1Lsi05G004970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009579 thylakoid