Lsi05G004610 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G004610
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionribonucleoside-diphosphate reductase small chain
Locationchr05: 5888414 .. 5889400 (-)
RNA-Seq ExpressionLsi05G004610
SyntenyLsi05G004610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGCAATTCCCGATGAGCCTCTCCTTGCCTCCAACCCCGATCGTTTCTGCATGTTCCCGATTCAGTATCCTCAAATCTGGGAGATGTACAAGAAAGCCGAAGCCTCCTTCTGGACTGCCGAGGAAGTCGATCTCTCACAGGACCTTCGCCATTGGGAATCCTTAACTTCCGACGAGAAACACTTCATCACTCACGTTCTTGCCTTCTTCGCTGCTTCTGATGGCATCGTTTTGGAGAATCTCGCCGGACGCTTCATGAAAGAAGTTCAAGTGGCTGAAGCCAGAGCCTTCTATGGATTCCAAATTGCGATCGAGAATATCCACTCTGAGATGTACAGTCTTCTCCTTGAAACCTATATCAAGGATTCGATTGAGAAGAATCGGTTATTCCGTGCTATTGAAACTGTCCCCTGCGTCAAGAAAAAGGCTGATTGGGCGTTGAAGTGGATTGACAGTACAGAATCGTTCGCTGAGCGGATTTTGGCGTTCGCTTGTGTTGAGGGAATTTTCTTCTCAGGTAGTTTCTGCTCTATTTTCTGGTTAAAAAAACGTGGGCTAATGCCTGGATTAACGTTCTCAAATGAGTTGATTTCAAGAGATGAAGGGCTTCATTGTGATTTTGCTTGTTTGTTGTATTCATTATTGAGGAAGAAACTGAGTGAGGATCGAGTGAAGGAGATTGTAACAGAGGCAGTAGATATAGAGAGGGAATTTGTGTGTGATGCGTTGCCAGTGGCTCTTGTTGGAATGAATGGGGATTTGATGAGCCAATACATAGAATTTGTGACAGATAGGTTGGTTGGAGCCTTAGGGTATGAGAAAATCTACAACGTTCAAAATCCATTTGATTGGATGGAGTTGATTTCATTGCAGGGGAAGACTAATTTCTTTGAGAAAAGAGTTGGTGAATATCAAAAGGCATCTGTGATGTCAAATTTAAATGGAAATGGGGGTCATGTGTTTAAGATGGATGAGGATTTCTGA

mRNA sequence

ATGCCTGCAATTCCCGATGAGCCTCTCCTTGCCTCCAACCCCGATCGTTTCTGCATGTTCCCGATTCAGTATCCTCAAATCTGGGAGATGTACAAGAAAGCCGAAGCCTCCTTCTGGACTGCCGAGGAAGTCGATCTCTCACAGGACCTTCGCCATTGGGAATCCTTAACTTCCGACGAGAAACACTTCATCACTCACGTTCTTGCCTTCTTCGCTGCTTCTGATGGCATCGTTTTGGAGAATCTCGCCGGACGCTTCATGAAAGAAGTTCAAGTGGCTGAAGCCAGAGCCTTCTATGGATTCCAAATTGCGATCGAGAATATCCACTCTGAGATGTACAGTCTTCTCCTTGAAACCTATATCAAGGATTCGATTGAGAAGAATCGGTTATTCCGTGCTATTGAAACTGTCCCCTGCGTCAAGAAAAAGGCTGATTGGGCGTTGAAGTGGATTGACAGTACAGAATCGTTCGCTGAGCGGATTTTGGCGTTCGCTTGTGTTGAGGGAATTTTCTTCTCAGGTAGTTTCTGCTCTATTTTCTGGTTAAAAAAACGTGGGCTAATGCCTGGATTAACGTTCTCAAATGAGTTGATTTCAAGAGATGAAGGGCTTCATTGTGATTTTGCTTGTTTGTTGTATTCATTATTGAGGAAGAAACTGAGTGAGGATCGAGTGAAGGAGATTGTAACAGAGGCAGTAGATATAGAGAGGGAATTTGTGTGTGATGCGTTGCCAGTGGCTCTTGTTGGAATGAATGGGGATTTGATGAGCCAATACATAGAATTTGTGACAGATAGGTTGGTTGGAGCCTTAGGGTATGAGAAAATCTACAACGTTCAAAATCCATTTGATTGGATGGAGTTGATTTCATTGCAGGGGAAGACTAATTTCTTTGAGAAAAGAGTTGGTGAATATCAAAAGGCATCTGTGATGTCAAATTTAAATGGAAATGGGGGTCATGTGTTTAAGATGGATGAGGATTTCTGA

Coding sequence (CDS)

ATGCCTGCAATTCCCGATGAGCCTCTCCTTGCCTCCAACCCCGATCGTTTCTGCATGTTCCCGATTCAGTATCCTCAAATCTGGGAGATGTACAAGAAAGCCGAAGCCTCCTTCTGGACTGCCGAGGAAGTCGATCTCTCACAGGACCTTCGCCATTGGGAATCCTTAACTTCCGACGAGAAACACTTCATCACTCACGTTCTTGCCTTCTTCGCTGCTTCTGATGGCATCGTTTTGGAGAATCTCGCCGGACGCTTCATGAAAGAAGTTCAAGTGGCTGAAGCCAGAGCCTTCTATGGATTCCAAATTGCGATCGAGAATATCCACTCTGAGATGTACAGTCTTCTCCTTGAAACCTATATCAAGGATTCGATTGAGAAGAATCGGTTATTCCGTGCTATTGAAACTGTCCCCTGCGTCAAGAAAAAGGCTGATTGGGCGTTGAAGTGGATTGACAGTACAGAATCGTTCGCTGAGCGGATTTTGGCGTTCGCTTGTGTTGAGGGAATTTTCTTCTCAGGTAGTTTCTGCTCTATTTTCTGGTTAAAAAAACGTGGGCTAATGCCTGGATTAACGTTCTCAAATGAGTTGATTTCAAGAGATGAAGGGCTTCATTGTGATTTTGCTTGTTTGTTGTATTCATTATTGAGGAAGAAACTGAGTGAGGATCGAGTGAAGGAGATTGTAACAGAGGCAGTAGATATAGAGAGGGAATTTGTGTGTGATGCGTTGCCAGTGGCTCTTGTTGGAATGAATGGGGATTTGATGAGCCAATACATAGAATTTGTGACAGATAGGTTGGTTGGAGCCTTAGGGTATGAGAAAATCTACAACGTTCAAAATCCATTTGATTGGATGGAGTTGATTTCATTGCAGGGGAAGACTAATTTCTTTGAGAAAAGAGTTGGTGAATATCAAAAGGCATCTGTGATGTCAAATTTAAATGGAAATGGGGGTCATGTGTTTAAGATGGATGAGGATTTCTGA

Protein sequence

MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDEKHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETYIKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFVCDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEKRVGEYQKASVMSNLNGNGGHVFKMDEDF
Homology
BLAST of Lsi05G004610 vs. ExPASy Swiss-Prot
Match: P49730 (Ribonucleoside-diphosphate reductase small chain OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.2e-167
Identity = 285/329 (86.63%), Postives = 309/329 (93.92%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MP IP+EPLLAS+PDRFCMFPIQYPQIWEMYKKA ASFWTAEEVDLS D RHWE+LT  E
Sbjct: 1   MPLIPEEPLLASSPDRFCMFPIQYPQIWEMYKKALASFWTAEEVDLSSDTRHWETLTPGE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           +HFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLE+Y
Sbjct: 61  RHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLESY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EK+RLFRA+ET PCV+KKA WAL+WID +E+FAER++AFACVEGIFFSGSFC+IF
Sbjct: 121 IKDSDEKSRLFRAVETNPCVEKKAKWALRWIDGSETFAERLVAFACVEGIFFSGSFCAIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLR KL+E+RVK IV +AV+IEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRTKLTEERVKGIVADAVEIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALP ALVGMNGDLMS+YIEFV DRL+ ALGY+K+YN QNPFDWMELISLQGKTNFFEK
Sbjct: 241 CDALPCALVGMNGDLMSKYIEFVADRLLDALGYDKLYNAQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVGEYQKASVMS+LNGNG  H FK+DEDF
Sbjct: 301 RVGEYQKASVMSSLNGNGATHEFKLDEDF 329

BLAST of Lsi05G004610 vs. ExPASy Swiss-Prot
Match: Q9LSD0 (Ribonucleoside-diphosphate reductase small chain C OS=Arabidopsis thaliana OX=3702 GN=TSO2 PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 6.9e-160
Identity = 273/332 (82.23%), Postives = 300/332 (90.36%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWE-SLTSD 60
           MP++P+EPLL   PDRFCMFPI YPQIWEMYKKAEASFWTAEEVDLSQD R WE SL   
Sbjct: 1   MPSMPEEPLLTPTPDRFCMFPIHYPQIWEMYKKAEASFWTAEEVDLSQDNRDWENSLNDG 60

Query: 61  EKHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLET 120
           E+HFI HVLAFFAASDGIVLENLA RFM +VQV+EARAFYGFQIAIENIHSEMYSLLL+T
Sbjct: 61  ERHFIKHVLAFFAASDGIVLENLASRFMSDVQVSEARAFYGFQIAIENIHSEMYSLLLDT 120

Query: 121 YIKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSI 180
           YIKD+ E++ LFRAIET+PCV KKA WA+KWID +++FAERI+AFACVEGIFFSGSFCSI
Sbjct: 121 YIKDNKERDHLFRAIETIPCVAKKAQWAMKWIDGSQTFAERIIAFACVEGIFFSGSFCSI 180

Query: 181 FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREF 240
           FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLY+LL+ KLSE+RVK IV +AV+IEREF
Sbjct: 181 FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYTLLKTKLSEERVKSIVCDAVEIEREF 240

Query: 241 VCDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFE 300
           VCDALP ALVGMN DLMSQYIEFV DRL+GALGY K+Y V NPFDWMELISLQGKTNFFE
Sbjct: 241 VCDALPCALVGMNRDLMSQYIEFVADRLLGALGYGKVYGVTNPFDWMELISLQGKTNFFE 300

Query: 301 KRVGEYQKASVMSNLNGNG---GHVFKMDEDF 329
           KRVG+YQKASVMS++NGNG    HVF +DEDF
Sbjct: 301 KRVGDYQKASVMSSVNGNGAFDNHVFSLDEDF 332

BLAST of Lsi05G004610 vs. ExPASy Swiss-Prot
Match: P0DKH3 (Ribonucleoside-diphosphate reductase small chain B OS=Arabidopsis thaliana OX=3702 GN=RNR2B PE=2 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 2.2e-158
Identity = 269/333 (80.78%), Postives = 302/333 (90.69%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWE-SLTSD 60
           MP++P+EP+L   PDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQD R WE SLT+D
Sbjct: 1   MPSMPEEPILTPTPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDNRDWENSLTND 60

Query: 61  EKHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLET 120
           E+HFI HVLAFFAASDGIVLENL+ RFM +VQ++EARAFYGFQIAIENIHSEMYSLLL+T
Sbjct: 61  ERHFIKHVLAFFAASDGIVLENLSTRFMSDVQISEARAFYGFQIAIENIHSEMYSLLLDT 120

Query: 121 YIKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSI 180
           YIKD+ E++ LFRAIET+PCV KKA+WA+KWI+ ++SFAERI+AFACVEGIFFSGSFCSI
Sbjct: 121 YIKDNKERDHLFRAIETIPCVTKKAEWAMKWINGSQSFAERIVAFACVEGIFFSGSFCSI 180

Query: 181 FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREF 240
           FWLKKRGLMPGLTFSNELISRDEGLHCDFACL+YSLLR KL EDR+K IV +AV+IEREF
Sbjct: 181 FWLKKRGLMPGLTFSNELISRDEGLHCDFACLIYSLLRTKLDEDRLKAIVCDAVEIEREF 240

Query: 241 VCDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFE 300
           VCDALP ALVGMN +LMSQYIEFV DRL+ ALG  K+Y V NPFDWMELISLQGKTNFFE
Sbjct: 241 VCDALPCALVGMNRELMSQYIEFVADRLLAALGCAKVYGVSNPFDWMELISLQGKTNFFE 300

Query: 301 KRVGEYQKASVMSNLNGNGG----HVFKMDEDF 329
           KRVGEYQKAS+MS+++GN      HVFK+DEDF
Sbjct: 301 KRVGEYQKASIMSSVHGNAAFNDDHVFKLDEDF 333

BLAST of Lsi05G004610 vs. ExPASy Swiss-Prot
Match: P50651 (Ribonucleoside-diphosphate reductase small chain A OS=Arabidopsis thaliana OX=3702 GN=RNR2A PE=1 SV=2)

HSP 1 Score: 505.4 bits (1300), Expect = 4.9e-142
Identity = 245/324 (75.62%), Postives = 277/324 (85.49%), Query Frame = 0

Query: 6   DEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDEKHFIT 65
           +EPLL +   RF MFPI+Y  IWEMYKKAEASFWTAEEVDLS D++ WE+LT  EKHFI+
Sbjct: 18  EEPLLMAQNQRFTMFPIRYKSIWEMYKKAEASFWTAEEVDLSTDVQQWEALTDSEKHFIS 77

Query: 66  HVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETYIKDSI 125
           H+LAFFAASDGIVLENLA RF+ +VQV EARAFYGFQIA+ENIHSEMYSLLLET+IKDS 
Sbjct: 78  HILAFFAASDGIVLENLAARFLNDVQVPEARAFYGFQIAMENIHSEMYSLLLETFIKDSK 137

Query: 126 EKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIFWLKKR 185
           EK+RLF AIET+PC+ KKA W L WI S  SFA R++AFACVEGIFFSGSFC+IFWLKKR
Sbjct: 138 EKDRLFNAIETIPCISKKAKWCLDWIQSPMSFAVRLVAFACVEGIFFSGSFCAIFWLKKR 197

Query: 186 GLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFVCDALP 245
           GLMPGLTFSNELISRDEGLHCDFACLLYSLL+K+L  ++V +IV EAV+IE EFVC ALP
Sbjct: 198 GLMPGLTFSNELISRDEGLHCDFACLLYSLLQKQLPLEKVYQIVHEAVEIETEFVCKALP 257

Query: 246 VALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEKRVGEY 305
             L+GMN +LMSQYI+FV DRL+  LG E+ Y  +NPFDWME ISLQGKTNFFEKRVGEY
Sbjct: 258 CDLIGMNSNLMSQYIQFVADRLLVTLGCERTYKAENPFDWMEFISLQGKTNFFEKRVGEY 317

Query: 306 QKASVMSNL-NGNGGHVFKMDEDF 329
           QKASVMSNL NGN  + F  +EDF
Sbjct: 318 QKASVMSNLQNGNQNYEFTTEEDF 341

BLAST of Lsi05G004610 vs. ExPASy Swiss-Prot
Match: Q4KLN6 (Ribonucleoside-diphosphate reductase subunit M2 OS=Rattus norvegicus OX=10116 GN=Rrm2 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.9e-133
Identity = 234/328 (71.34%), Postives = 272/328 (82.93%), Query Frame = 0

Query: 2   PAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDEK 61
           P+I +EPLL  NP RF +FPI+Y  IW+MYKKAEASFWTAEEVDLS+D++HWE+L  DE+
Sbjct: 66  PSIEEEPLLRENPRRFVVFPIEYHDIWQMYKKAEASFWTAEEVDLSKDIQHWEALKPDER 125

Query: 62  HFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETYI 121
           HFI+HVLAFFAASDGIV ENL  RF +EVQV EAR FYGFQIA+ENIHSEMYSLL++TYI
Sbjct: 126 HFISHVLAFFAASDGIVNENLVERFSQEVQVTEARCFYGFQIAMENIHSEMYSLLIDTYI 185

Query: 122 KDSIEKNRLFRAIETVPCVKKKADWALKWI-DSTESFAERILAFACVEGIFFSGSFCSIF 181
           KDS E+  LF AIET+PCVKKKADWAL+WI D   ++ ER++AFA VEGIFFSGSF SIF
Sbjct: 186 KDSKEREYLFNAIETMPCVKKKADWALRWIGDKEATYGERVVAFAAVEGIFFSGSFASIF 245

Query: 182 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 241
           WLKKRGLMPGLTFSNELISRDEGLHCDFACL++  L  K SE RVKEI+T +V IE+EF+
Sbjct: 246 WLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKHLVHKPSEQRVKEIITNSVRIEQEFL 305

Query: 242 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 301
            +ALPV L+GMN  LM QYIEFV DRL+  LG+ KI+ V+NPFD+ME ISL+GKTNFFEK
Sbjct: 306 TEALPVKLIGMNCTLMKQYIEFVADRLMLELGFNKIFKVENPFDFMENISLEGKTNFFEK 365

Query: 302 RVGEYQKASVMSNLNGNGGHVFKMDEDF 329
           RVGEYQ+  VMSN   N    F +D DF
Sbjct: 366 RVGEYQRMGVMSNSTENS---FTLDADF 390

BLAST of Lsi05G004610 vs. ExPASy TrEMBL
Match: A0A6J1F5K5 (ribonucleoside-diphosphate reductase small chain OS=Cucurbita moschata OX=3662 GN=LOC111442318 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.8e-180
Identity = 316/329 (96.05%), Postives = 321/329 (97.57%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPAIPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE
Sbjct: 1   MPAIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EK+RLFRAIETVPCVKKKADWALKWIDSTE+FAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNEKSRLFRAIETVPCVKKKADWALKWIDSTETFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           C+ALPVALVGMNGDLMSQYIEFV DRLVGALGY KIYN QNPFDWMELISLQGKTNFFEK
Sbjct: 241 CEALPVALVGMNGDLMSQYIEFVADRLVGALGYGKIYNAQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVG+YQKASVMSNLNG+G  H FKMDEDF
Sbjct: 301 RVGDYQKASVMSNLNGHGDTHEFKMDEDF 329

BLAST of Lsi05G004610 vs. ExPASy TrEMBL
Match: A0A0A0L8R8 (Ribonucleoside-diphosphate reductase small chain OS=Cucumis sativus OX=3659 GN=Csa_3G597320 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.1e-179
Identity = 313/329 (95.14%), Postives = 319/329 (96.96%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MP+IPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQD RHW+SLTSDE
Sbjct: 1   MPSIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDTRHWDSLTSDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAE RAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEVRAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EKNRLF AI+TVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNEKNRLFHAIDTVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKL+ DRVKEIV EAV+IEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLNVDRVKEIVMEAVNIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALPVALVGMNGDLMSQYIEFV DRLVGALGY+KIYNVQNPFDWMELISLQGKTNFFEK
Sbjct: 241 CDALPVALVGMNGDLMSQYIEFVADRLVGALGYDKIYNVQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVGEYQKASVMSNLNGNGG H FKMDEDF
Sbjct: 301 RVGEYQKASVMSNLNGNGGIHEFKMDEDF 329

BLAST of Lsi05G004610 vs. ExPASy TrEMBL
Match: A0A1S3BNB8 (ribonucleoside-diphosphate reductase small chain OS=Cucumis melo OX=3656 GN=LOC103491720 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.3e-179
Identity = 312/329 (94.83%), Postives = 320/329 (97.26%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MP+IPDEPLLA NPDRFCMFPIQYPQIWEMYKKA ASFWTAEEVDLSQDLRHW+SLTSDE
Sbjct: 1   MPSIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKALASFWTAEEVDLSQDLRHWDSLTSDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFIT+VLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQI IENIHSEMYSLLLETY
Sbjct: 61  KHFITYVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQICIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EKNRLF AI+TVPCVKKKADWALKWIDST+SFAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSSEKNRLFHAIDTVPCVKKKADWALKWIDSTDSFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRV++IVTEAVDIEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVRQIVTEAVDIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALPVALVGMNGDLMSQYIEFV DRLVG LGYEKIYNVQNPFDWMELISLQGKTNFFEK
Sbjct: 241 CDALPVALVGMNGDLMSQYIEFVADRLVGTLGYEKIYNVQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVGEYQKASVMS+LNGNGG H FKMDEDF
Sbjct: 301 RVGEYQKASVMSSLNGNGGIHEFKMDEDF 329

BLAST of Lsi05G004610 vs. ExPASy TrEMBL
Match: A0A6J1J9K8 (ribonucleoside-diphosphate reductase small chain OS=Cucurbita maxima OX=3661 GN=LOC111482520 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 6.9e-179
Identity = 315/329 (95.74%), Postives = 317/329 (96.35%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPAIPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLT DE
Sbjct: 1   MPAIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTFDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EKN LFRAIETVPCVKKKADWALKWIDS E+FAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNEKNLLFRAIETVPCVKKKADWALKWIDSPETFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALPVALVGMNGDLMSQYIEFV DRLVGALGY KIYN  NPFDWMELISLQGKTNFFEK
Sbjct: 241 CDALPVALVGMNGDLMSQYIEFVADRLVGALGYGKIYNALNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVG+YQKASVMSNLNGNG  H FKMDEDF
Sbjct: 301 RVGDYQKASVMSNLNGNGDTHEFKMDEDF 329

BLAST of Lsi05G004610 vs. ExPASy TrEMBL
Match: A0A6P4AGQ7 (ribonucleoside-diphosphate reductase small chain OS=Ziziphus jujuba OX=326968 GN=LOC107427918 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.3e-173
Identity = 298/329 (90.58%), Postives = 316/329 (96.05%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPA P+EPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQD+RHWE+LT DE
Sbjct: 75  MPAYPEEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDVRHWENLTPDE 134

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFI+HVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 135 KHFISHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 194

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKD +EKNRLF AIETVPCV KKA+WAL+WID TESFAER++AFACVEGIFFSGSFC+IF
Sbjct: 195 IKDPVEKNRLFHAIETVPCVAKKAEWALRWIDGTESFAERLVAFACVEGIFFSGSFCAIF 254

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLY+L+RKKLSE+RVK IV +AVDIEREFV
Sbjct: 255 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYTLMRKKLSEERVKGIVRDAVDIEREFV 314

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALP ALVGMNGDLMSQYIEFV DRL+GALGY KIYNVQNPFDWMELISLQGKTNFFEK
Sbjct: 315 CDALPCALVGMNGDLMSQYIEFVADRLLGALGYGKIYNVQNPFDWMELISLQGKTNFFEK 374

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVGEYQKASVMS++NG+GG HVFKMDEDF
Sbjct: 375 RVGEYQKASVMSSINGDGGTHVFKMDEDF 403

BLAST of Lsi05G004610 vs. NCBI nr
Match: KAG6581100.1 (hypothetical protein SDJN03_21102, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017830.1 hypothetical protein SDJN02_19696, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641.7 bits (1654), Expect = 3.4e-180
Identity = 316/329 (96.05%), Postives = 321/329 (97.57%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPAIPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE
Sbjct: 1   MPAIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS +K+RLFRAIETVPCVKKKADWALKWIDSTE+FAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNDKSRLFRAIETVPCVKKKADWALKWIDSTETFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           C+ALPVALVGMNGDLMSQYIEFV DRLVGALGY KIYN QNPFDWMELISLQGKTNFFEK
Sbjct: 241 CEALPVALVGMNGDLMSQYIEFVADRLVGALGYGKIYNAQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVG+YQKASVMSNLNGNG  H FKMDEDF
Sbjct: 301 RVGDYQKASVMSNLNGNGDTHEFKMDEDF 329

BLAST of Lsi05G004610 vs. NCBI nr
Match: XP_023527500.1 (ribonucleoside-diphosphate reductase small chain [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.3 bits (1653), Expect = 4.4e-180
Identity = 317/329 (96.35%), Postives = 320/329 (97.26%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPAIPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE
Sbjct: 76  MPAIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 135

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 136 KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 195

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EK+RLFRAIETVPCVKKKADWALKWIDSTE+FAERILAFACVEGIFFSGSFCSIF
Sbjct: 196 IKDSNEKSRLFRAIETVPCVKKKADWALKWIDSTETFAERILAFACVEGIFFSGSFCSIF 255

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLL KKLSEDRVKEIVTEAVDIEREFV
Sbjct: 256 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLWKKLSEDRVKEIVTEAVDIEREFV 315

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALPVALVGMNGDLMSQYIEFV DRLVGALGY KIYN QNPFDWMELISLQGKTNFFEK
Sbjct: 316 CDALPVALVGMNGDLMSQYIEFVADRLVGALGYGKIYNAQNPFDWMELISLQGKTNFFEK 375

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVG+YQKASVMSNLNGNG  H FKMDEDF
Sbjct: 376 RVGDYQKASVMSNLNGNGDTHEFKMDEDF 404

BLAST of Lsi05G004610 vs. NCBI nr
Match: XP_022935447.1 (ribonucleoside-diphosphate reductase small chain [Cucurbita moschata])

HSP 1 Score: 641.0 bits (1652), Expect = 5.8e-180
Identity = 316/329 (96.05%), Postives = 321/329 (97.57%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPAIPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE
Sbjct: 1   MPAIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EK+RLFRAIETVPCVKKKADWALKWIDSTE+FAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNEKSRLFRAIETVPCVKKKADWALKWIDSTETFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           C+ALPVALVGMNGDLMSQYIEFV DRLVGALGY KIYN QNPFDWMELISLQGKTNFFEK
Sbjct: 241 CEALPVALVGMNGDLMSQYIEFVADRLVGALGYGKIYNAQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVG+YQKASVMSNLNG+G  H FKMDEDF
Sbjct: 301 RVGDYQKASVMSNLNGHGDTHEFKMDEDF 329

BLAST of Lsi05G004610 vs. NCBI nr
Match: XP_038878212.1 (ribonucleoside-diphosphate reductase small chain [Benincasa hispida])

HSP 1 Score: 638.6 bits (1646), Expect = 2.9e-179
Identity = 313/329 (95.14%), Postives = 321/329 (97.57%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MPAIPDEPLLASNPDRFCMFPI YP+IW+MYKKAEASFWTAEEVDLSQDLRHWESLT+DE
Sbjct: 1   MPAIPDEPLLASNPDRFCMFPIHYPEIWKMYKKAEASFWTAEEVDLSQDLRHWESLTADE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EKNRLF AIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNEKNRLFHAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSE+R++EIV EAVDIEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEERLREIVMEAVDIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALPVAL+GMNGDLMSQYIEFV DRLVG LGYEKIYNVQNPFDWMELISLQGKTNFFEK
Sbjct: 241 CDALPVALIGMNGDLMSQYIEFVADRLVGELGYEKIYNVQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVGEYQKASVMS+LNGN G HVFKMDEDF
Sbjct: 301 RVGEYQKASVMSSLNGNEGIHVFKMDEDF 329

BLAST of Lsi05G004610 vs. NCBI nr
Match: XP_004145693.1 (ribonucleoside-diphosphate reductase small chain [Cucumis sativus] >KGN58233.1 hypothetical protein Csa_017522 [Cucumis sativus])

HSP 1 Score: 637.5 bits (1643), Expect = 6.4e-179
Identity = 313/329 (95.14%), Postives = 319/329 (96.96%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDE 60
           MP+IPDEPLLA NPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQD RHW+SLTSDE
Sbjct: 1   MPSIPDEPLLAPNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDTRHWDSLTSDE 60

Query: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETY 120
           KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAE RAFYGFQIAIENIHSEMYSLLLETY
Sbjct: 61  KHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEVRAFYGFQIAIENIHSEMYSLLLETY 120

Query: 121 IKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180
           IKDS EKNRLF AI+TVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF
Sbjct: 121 IKDSNEKNRLFHAIDTVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIF 180

Query: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFV 240
           WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKL+ DRVKEIV EAV+IEREFV
Sbjct: 181 WLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLNVDRVKEIVMEAVNIEREFV 240

Query: 241 CDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEK 300
           CDALPVALVGMNGDLMSQYIEFV DRLVGALGY+KIYNVQNPFDWMELISLQGKTNFFEK
Sbjct: 241 CDALPVALVGMNGDLMSQYIEFVADRLVGALGYDKIYNVQNPFDWMELISLQGKTNFFEK 300

Query: 301 RVGEYQKASVMSNLNGNGG-HVFKMDEDF 329
           RVGEYQKASVMSNLNGNGG H FKMDEDF
Sbjct: 301 RVGEYQKASVMSNLNGNGGIHEFKMDEDF 329

BLAST of Lsi05G004610 vs. TAIR 10
Match: AT3G27060.1 (Ferritin/ribonucleotide reductase-like family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 4.9e-161
Identity = 273/332 (82.23%), Postives = 300/332 (90.36%), Query Frame = 0

Query: 1   MPAIPDEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWE-SLTSD 60
           MP++P+EPLL   PDRFCMFPI YPQIWEMYKKAEASFWTAEEVDLSQD R WE SL   
Sbjct: 1   MPSMPEEPLLTPTPDRFCMFPIHYPQIWEMYKKAEASFWTAEEVDLSQDNRDWENSLNDG 60

Query: 61  EKHFITHVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLET 120
           E+HFI HVLAFFAASDGIVLENLA RFM +VQV+EARAFYGFQIAIENIHSEMYSLLL+T
Sbjct: 61  ERHFIKHVLAFFAASDGIVLENLASRFMSDVQVSEARAFYGFQIAIENIHSEMYSLLLDT 120

Query: 121 YIKDSIEKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSI 180
           YIKD+ E++ LFRAIET+PCV KKA WA+KWID +++FAERI+AFACVEGIFFSGSFCSI
Sbjct: 121 YIKDNKERDHLFRAIETIPCVAKKAQWAMKWIDGSQTFAERIIAFACVEGIFFSGSFCSI 180

Query: 181 FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREF 240
           FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLY+LL+ KLSE+RVK IV +AV+IEREF
Sbjct: 181 FWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYTLLKTKLSEERVKSIVCDAVEIEREF 240

Query: 241 VCDALPVALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFE 300
           VCDALP ALVGMN DLMSQYIEFV DRL+GALGY K+Y V NPFDWMELISLQGKTNFFE
Sbjct: 241 VCDALPCALVGMNRDLMSQYIEFVADRLLGALGYGKVYGVTNPFDWMELISLQGKTNFFE 300

Query: 301 KRVGEYQKASVMSNLNGNG---GHVFKMDEDF 329
           KRVG+YQKASVMS++NGNG    HVF +DEDF
Sbjct: 301 KRVGDYQKASVMSSVNGNGAFDNHVFSLDEDF 332

BLAST of Lsi05G004610 vs. TAIR 10
Match: AT3G23580.1 (ribonucleotide reductase 2A )

HSP 1 Score: 505.4 bits (1300), Expect = 3.5e-143
Identity = 245/324 (75.62%), Postives = 277/324 (85.49%), Query Frame = 0

Query: 6   DEPLLASNPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDLRHWESLTSDEKHFIT 65
           +EPLL +   RF MFPI+Y  IWEMYKKAEASFWTAEEVDLS D++ WE+LT  EKHFI+
Sbjct: 18  EEPLLMAQNQRFTMFPIRYKSIWEMYKKAEASFWTAEEVDLSTDVQQWEALTDSEKHFIS 77

Query: 66  HVLAFFAASDGIVLENLAGRFMKEVQVAEARAFYGFQIAIENIHSEMYSLLLETYIKDSI 125
           H+LAFFAASDGIVLENLA RF+ +VQV EARAFYGFQIA+ENIHSEMYSLLLET+IKDS 
Sbjct: 78  HILAFFAASDGIVLENLAARFLNDVQVPEARAFYGFQIAMENIHSEMYSLLLETFIKDSK 137

Query: 126 EKNRLFRAIETVPCVKKKADWALKWIDSTESFAERILAFACVEGIFFSGSFCSIFWLKKR 185
           EK+RLF AIET+PC+ KKA W L WI S  SFA R++AFACVEGIFFSGSFC+IFWLKKR
Sbjct: 138 EKDRLFNAIETIPCISKKAKWCLDWIQSPMSFAVRLVAFACVEGIFFSGSFCAIFWLKKR 197

Query: 186 GLMPGLTFSNELISRDEGLHCDFACLLYSLLRKKLSEDRVKEIVTEAVDIEREFVCDALP 245
           GLMPGLTFSNELISRDEGLHCDFACLLYSLL+K+L  ++V +IV EAV+IE EFVC ALP
Sbjct: 198 GLMPGLTFSNELISRDEGLHCDFACLLYSLLQKQLPLEKVYQIVHEAVEIETEFVCKALP 257

Query: 246 VALVGMNGDLMSQYIEFVTDRLVGALGYEKIYNVQNPFDWMELISLQGKTNFFEKRVGEY 305
             L+GMN +LMSQYI+FV DRL+  LG E+ Y  +NPFDWME ISLQGKTNFFEKRVGEY
Sbjct: 258 CDLIGMNSNLMSQYIQFVADRLLVTLGCERTYKAENPFDWMEFISLQGKTNFFEKRVGEY 317

Query: 306 QKASVMSNL-NGNGGHVFKMDEDF 329
           QKASVMSNL NGN  + F  +EDF
Sbjct: 318 QKASVMSNLQNGNQNYEFTTEEDF 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P497301.2e-16786.63Ribonucleoside-diphosphate reductase small chain OS=Nicotiana tabacum OX=4097 PE... [more]
Q9LSD06.9e-16082.23Ribonucleoside-diphosphate reductase small chain C OS=Arabidopsis thaliana OX=37... [more]
P0DKH32.2e-15880.78Ribonucleoside-diphosphate reductase small chain B OS=Arabidopsis thaliana OX=37... [more]
P506514.9e-14275.62Ribonucleoside-diphosphate reductase small chain A OS=Arabidopsis thaliana OX=37... [more]
Q4KLN61.9e-13371.34Ribonucleoside-diphosphate reductase subunit M2 OS=Rattus norvegicus OX=10116 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1F5K52.8e-18096.05ribonucleoside-diphosphate reductase small chain OS=Cucurbita moschata OX=3662 G... [more]
A0A0A0L8R83.1e-17995.14Ribonucleoside-diphosphate reductase small chain OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BNB85.3e-17994.83ribonucleoside-diphosphate reductase small chain OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1J9K86.9e-17995.74ribonucleoside-diphosphate reductase small chain OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6P4AGQ73.3e-17390.58ribonucleoside-diphosphate reductase small chain OS=Ziziphus jujuba OX=326968 GN... [more]
Match NameE-valueIdentityDescription
KAG6581100.13.4e-18096.05hypothetical protein SDJN03_21102, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023527500.14.4e-18096.35ribonucleoside-diphosphate reductase small chain [Cucurbita pepo subsp. pepo][more]
XP_022935447.15.8e-18096.05ribonucleoside-diphosphate reductase small chain [Cucurbita moschata][more]
XP_038878212.12.9e-17995.14ribonucleoside-diphosphate reductase small chain [Benincasa hispida][more]
XP_004145693.16.4e-17995.14ribonucleoside-diphosphate reductase small chain [Cucumis sativus] >KGN58233.1 h... [more]
Match NameE-valueIdentityDescription
AT3G27060.14.9e-16182.23Ferritin/ribonucleotide reductase-like family protein [more]
AT3G23580.13.5e-14375.62ribonucleotide reductase 2A [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000358Ribonucleotide reductase small subunit familyPFAMPF00268Ribonuc_red_smcoord: 16..282
e-value: 5.1E-117
score: 390.3
IPR000358Ribonucleotide reductase small subunit familyPANTHERPTHR23409RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAINcoord: 6..328
IPR012348Ribonucleotide reductase-likeGENE3D1.10.620.20Ribonucleotide Reductase, subunit Acoord: 3..307
e-value: 5.6E-132
score: 441.4
NoneNo IPR availablePIRSRPIRSR000355-2PIRSR000355-2coord: 20..312
e-value: 1.2E-65
score: 219.9
NoneNo IPR availablePANTHERPTHR23409:SF38RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAINcoord: 6..328
IPR030475Ribonucleotide reductase small subunit, acitve sitePROSITEPS00368RIBORED_SMALLcoord: 105..121
IPR033909Ribonucleotide reductase small subunitCDDcd01049RNRR2coord: 17..291
e-value: 2.68543E-138
score: 391.603
IPR009078Ferritin-like superfamilySUPERFAMILY47240Ferritin-likecoord: 5..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G004610.1Lsi05G004610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009263 deoxyribonucleotide biosynthetic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
molecular_function GO:0016491 oxidoreductase activity